#METABOLOMICS WORKBENCH araskind_20170802_185111 DATATRACK_ID:1189 STUDY_ID:ST000838 ANALYSIS_ID:AN001343 PROJECT_ID:PR000598 VERSION 1 CREATED_ON August 9, 2017, 10:02 am #PROJECT PR:PROJECT_TITLE Obesity and marrow and MAT metabolomics Studies PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY MAT and bone marrow collected to evaluate lipid content/lipid type. In addition, PR:PROJECT_SUMMARY marrow evaluations for glycolysis/TCA cycle and acylcarnitines will be PR:PROJECT_SUMMARY performed. PR:INSTITUTE University of Michigan PR:DEPARTMENT Pedatric Endocrinology PR:LABORATORY Singer Lab PR:LAST_NAME Singer PR:FIRST_NAME Kanakadurga PR:ADDRESS Ann Arbor, MI PR:EMAIL ksinger@umich.edu PR:PHONE 734-764-5175 #STUDY ST:STUDY_TITLE Obesity and marrow and marrow adipose tissue (MAT) metabolomics ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY Male C57Bl6j mice were fed HFD for 16-20 weeks and marrow adipose tissue (MAT) ST:STUDY_SUMMARY and bone marrow collected to evaluate lipid content/lipid type. In addition, ST:STUDY_SUMMARY marrow evaluations for glycolysis/TCA cycle and acylcarnitines will be ST:STUDY_SUMMARY performed. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 6 #SUBJECT SU:SUBJECT_TYPE MOUSE SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0018085 S00028167 Diet:ND | Sample type:Adipose Tissue SUBJECT_SAMPLE_FACTORS SU0018086 S00028168 Diet:ND | Sample type:Adipose Tissue SUBJECT_SAMPLE_FACTORS SU0018087 S00028169 Diet:ND | Sample type:Adipose Tissue SUBJECT_SAMPLE_FACTORS SU0018088 S00028170 Diet:HFD | Sample type:Adipose Tissue SUBJECT_SAMPLE_FACTORS SU0018089 S00028171 Diet:HFD | Sample type:Adipose Tissue SUBJECT_SAMPLE_FACTORS SU0018090 S00028172 Diet:HFD | Sample type:Adipose Tissue SUBJECT_SAMPLE_FACTORS SU0018085 S00028173 Diet:ND | Sample type:Bone Marrow SUBJECT_SAMPLE_FACTORS SU0018086 S00028174 Diet:ND | Sample type:Bone Marrow SUBJECT_SAMPLE_FACTORS SU0018087 S00028175 Diet:ND | Sample type:Bone Marrow SUBJECT_SAMPLE_FACTORS SU0018088 S00028176 Diet:HFD | Sample type:Bone Marrow SUBJECT_SAMPLE_FACTORS SU0018089 S00028177 Diet:HFD | Sample type:Bone Marrow SUBJECT_SAMPLE_FACTORS SU0018090 S00028178 Diet:HFD | Sample type:Bone Marrow #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A008_Acyl_Carnitines.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Agilent CH:COLUMN_NAME Phenomenex Luna NH2 (150 x 1mm, 3um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A011 - Glycolysis-TCA-nucleotides.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6520B QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pmol/mg MS_METABOLITE_DATA_START Samples S00028173 S00028174 S00028175 S00028176 S00028177 S00028178 Factors Diet:ND | Sample type:Bone Marrow Diet:ND | Sample type:Bone Marrow Diet:ND | Sample type:Bone Marrow Diet:HFD | Sample type:Bone Marrow Diet:HFD | Sample type:Bone Marrow Diet:HFD | Sample type:Bone Marrow D-ERYTHROSE 4-PHOSPHATE 11.21723691 9.525160339 10.81684136 8.269531081 8.151496331 10.22268419 BETA-NAD+ .788106171 1.207061422 1.468657745 1.076459844 1.419506204 1.052288221 COENZYME II .017117051 .11518378 .041070733 .094748594 .035607993 SEDOHEPTULOSE 7-PHOSPHATE .971134884 .840626295 1.05661219 1.009608077 1.137475325 1.282093561 D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE) .630753035 .596264445 .703219495 .641354059 1.00400696 1.00731512 CITRIC ACID 5.651720426 6.966896298 1.124487197 7.918843951 15.89988046 12.73387098 NADH 1.504960037 1.498680917 2.991418756 3.022317975 2.951316153 2.606690303 FAD .621885165 .621454168 .739493275 .698121132 .562010923 .591030929 MALIC ACID 37.45169615 40.77990219 49.12109287 46.45408942 43.99050055 45.82820688 ADENOSINE TRIPHOSPHATE .289752498 .247268357 .233750701 .241138066 .65733375 .746327059 ROBISON ESTER 11.18826166 8.340274486 10.36384947 7.181603963 7.25749618 8.655829603 SUCROSE 4.483223877 7.537540425 12.23803578 7.935353572 13.41960018 9.935247418 ADENOSINE 5'-DIPHOSPHATE 1.758209907 .955810075 1.981040265 3.185868953 4.764500714 4.284073747 ADENOSINE 5'-MONOPHOSPHATE 35.31938586 42.85794676 52.68821956 51.49928332 52.35454546 55.88417157 GLYCERATE 3-PHOSPHATE 3.083440903 3.131922324 1.420333692 3.458399698 3.790107816 3.186606599 RIBOSE PHOSPHATE 5.616352061 5.112114499 7.189463303 7.980978684 7.455770943 8.366128738 6-PHOSPHOGLUCONIC ACID .744591739 .73717619 .766298984 .549127899 .557499085 .654940072 ACETOACETYL-COA .0733146 .088486978 .09308221 .151107766 .142492041 .18864003 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type D-ERYTHROSE 4-PHOSPHATE 122357 BETA-NAD+ 15938971 COENZYME II 15938972 SEDOHEPTULOSE 7-PHOSPHATE 165007 D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE) 21125049 CITRIC ACID 311 NADH 439153 FAD 46906035 MALIC ACID 525 ADENOSINE TRIPHOSPHATE 5957 ROBISON ESTER 5958 SUCROSE 5988 ADENOSINE 5'-DIPHOSPHATE 6022 ADENOSINE 5'-MONOPHOSPHATE 6083 GLYCERATE 3-PHOSPHATE 724 RIBOSE PHOSPHATE 77982 6-PHOSPHOGLUCONIC ACID 91493 ACETOACETYL-COA 92153 METABOLITES_END #END