#METABOLOMICS WORKBENCH araskind_20170802_185416 DATATRACK_ID:1190 STUDY_ID:ST000839 ANALYSIS_ID:AN001345 PROJECT_ID:PR000542 VERSION 1 CREATED_ON August 9, 2017, 10:55 am #PROJECT PR:PROJECT_TITLE Thermal Modalities Study PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY This study is looking at the effect of cooling on muscle tissue metabolism PR:INSTITUTE University of Michigan PR:DEPARTMENT Orthopaedic Surgery PR:LABORATORY Mendias Lab PR:LAST_NAME Mendias PR:FIRST_NAME Christopher PR:ADDRESS Ann Arbor, MI PR:EMAIL cmendias@umich.edu PR:PHONE 734-764-3250 #STUDY ST:STUDY_TITLE Effect of cooling on muscle tissue metabolism (part II) ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY Thermal modalities are commonly used in sports medicine to affect tissue ST:STUDY_SUMMARY healing. Cold therapy is commonly used modalities, but the metabolic changes to ST:STUDY_SUMMARY muscle after cooling are not known. The objective of this study is to look at ST:STUDY_SUMMARY the effect of cooling on muscle metabolites and gene expression. There are a ST:STUDY_SUMMARY total of 8 subjects in the study. Each subject had an ice cup cryotherapy ST:STUDY_SUMMARY treatment ("cool" samples) to one leg for 15 minutes, and the other leg served ST:STUDY_SUMMARY as the control ("cntrl" samples). Two hours after the application of ST:STUDY_SUMMARY cryotherapy, a biopsy was taken from each thigh muscle. Muscle was minced with ST:STUDY_SUMMARY scissors and quickly snap frozen in liquid nitrogen. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 10 ST:TOTAL_SUBJECTS 8 ST:STUDY_COMMENTS We would like to run the TCA Plus Analysis on each of the samples. For the ST:STUDY_COMMENTS statistical analysis, we would normalize the cool side to the ctrl side, and ST:STUDY_COMMENTS look at the relative change in metabolite abundance as a result of the cooling ST:STUDY_COMMENTS procedure. only used in sports medicine to affect tissue healing. #SUBJECT SU:SUBJECT_TYPE HUMAN SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0014908 S00023215 Temperature:Control Subject=1 SUBJECT_SAMPLE_FACTORS SU0014908 S00023216 Temperature:Cooled Subject=1 SUBJECT_SAMPLE_FACTORS SU0014909 S00023217 Temperature:Control Subject=2 SUBJECT_SAMPLE_FACTORS SU0014909 S00023218 Temperature:Cooled Subject=2 SUBJECT_SAMPLE_FACTORS SU0014910 S00023219 Temperature:Control Subject=3 SUBJECT_SAMPLE_FACTORS SU0014910 S00023220 Temperature:Cooled Subject=3 SUBJECT_SAMPLE_FACTORS SU0014911 S00023221 Temperature:Control Subject=4 SUBJECT_SAMPLE_FACTORS SU0014911 S00023222 Temperature:Cooled Subject=4 SUBJECT_SAMPLE_FACTORS SU0014912 S00023223 Temperature:Control Subject=5 SUBJECT_SAMPLE_FACTORS SU0014912 S00023224 Temperature:Cooled Subject=5 SUBJECT_SAMPLE_FACTORS SU0014913 S00023225 Temperature:Control Subject=6 SUBJECT_SAMPLE_FACTORS SU0014913 S00023226 Temperature:Cooled Subject=6 SUBJECT_SAMPLE_FACTORS SU0014914 S00023227 Temperature:Control Subject=7 SUBJECT_SAMPLE_FACTORS SU0014914 S00023228 Temperature:Cooled Subject=7 SUBJECT_SAMPLE_FACTORS SU0014915 S00023229 Temperature:Control Subject=8 SUBJECT_SAMPLE_FACTORS SU0014915 S00023230 Temperature:Cooled Subject=8 #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A011_Glycolysis-TCA-nucleotides.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Agilent CH:COLUMN_NAME Phenomenex Luna NH2 (150 x 1mm, 3um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A011 - Glycolysis-TCA-nucleotides.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6520B QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nmol/mg MS_METABOLITE_DATA_START Samples S00023215 S00023216 S00023217 S00023218 S00023219 S00023220 S00023221 S00023222 S00023223 S00023224 S00023225 S00023226 S00023227 S00023228 S00023229 S00023230 Factors Temperature:Control Temperature:Cooled Temperature:Control Temperature:Cooled Temperature:Control Temperature:Cooled Temperature:Control Temperature:Cooled Temperature:Control Temperature:Cooled Temperature:Control Temperature:Cooled Temperature:Control Temperature:Cooled Temperature:Control Temperature:Cooled D-ERYTHROSE 4-PHOSPHATE 3.94646415 5.227754209 3.417357853 1.933654392 3.312279724 4.009106573 3.87202955 5.005469238 3.25329155 2.858650942 3.227766298 2.545112891 4.389657105 2.300372952 3.694400552 3.074067477 BETA-NAD+ .061383361 .094627856 .027148456 .016684607 .045028167 .011073473 .036870057 .027876861 .063795149 .035547528 .046566157 .108090497 .014606847 .077303078 .095719458 .108653926 COENZYME II .008207605 .006789212 .003144356 .002838797 .004040534 .002803397 .004234083 .00529144 .004647351 .003184894 .006466174 .005539331 .002119936 .004692058 .009093466 .010877024 SEDOHEPTULOSE 7-PHOSPHATE .00386658 .011162527 .002623441 .006653065 .002408949 .005335913 .003042897 .005404464 .003185302 .002275792 .007054431 .005453507 .008057386 .0035053 .003647809 .003262038 D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE) 1.269638109 .947416422 3.337867273 2.581308631 2.3273682 5.461056018 2.510364683 3.340046292 1.443928121 1.403804465 2.041791959 .781649437 1.843231206 .798620372 1.499817772 2.122147139 CITRIC ACID .081620299 .11481453 .066870211 .101163017 .152750345 .069848955 .08596618 .078378802 .162370123 .104068247 .182294429 .212417167 .063425171 .090186501 .260857311 .265800033 NADH .034282857 .045283412 .008360933 .030189146 .004401122 .07944624 .143262691 .078200061 .039511913 .176692706 .037573807 .07442283 FAD .010692553 .009597777 .007229298 .006092064 .00745574 .008345892 .007812437 .008195953 .007575984 .005975604 .008802507 .007117713 .006868486 .005984764 .013238667 .012357041 MALIC ACID 2.263102004 2.352403016 .894772328 .684167038 .761412689 .583195476 .914080484 .912978189 1.509675156 .758849478 2.525787507 3.076414962 1.037029319 .928601608 3.834833377 2.94478518 ADENOSINE TRIPHOSPHATE 2.519619294 2.556889038 .577001218 1.089223982 1.759796408 .392326337 2.026415602 1.411050083 2.316626289 1.5867462 2.261485404 2.264618889 .069146763 1.926661601 3.712126911 4.320597983 ROBISON ESTER 5.179479675 7.504835951 5.912119801 2.895636804 4.554954998 6.146420964 5.195052378 7.166376134 4.079391887 4.057755034 3.862134473 2.506050855 6.889751917 3.182842958 5.05705298 3.855391538 SUCROSE .169419199 .216134305 .295964022 .064927787 .047655018 .095066608 .086963055 .148015296 .080620477 .055596389 .105486143 .11323052 .389051843 .075397519 .176821841 .172603545 ADENOSINE 5'-DIPHOSPHATE .233946275 .239159323 .235614242 .210016908 .166813823 .325091614 .240027999 .227609424 .097709442 .095601901 .159951895 .109053017 .247691503 .100201306 .157065873 .178207686 ADENOSINE 5'-MONOPHOSPHATE .006770042 .009717114 .022453876 .010257355 .007842336 .015638742 .012829213 .015617282 .00833924 .007443757 .00612996 .005996644 .040161748 .008225998 .011075174 .013638223 GLYCERATE 3-PHOSPHATE 1.849566003 1.424811546 .512046675 .606554663 .724307902 .341281053 .525315952 .866275454 .58941218 .731903207 1.592912412 1.290033333 .249406339 .749362426 1.874208785 2.175277715 RIBOSE PHOSPHATE .172536194 .152353992 .200054901 .150771251 .097546661 .200870874 .116652625 .203337117 .073111602 .095920726 .162116805 .100856198 .406365077 .068877287 .131898038 .107317074 6-PHOSPHOGLUCONIC ACID .019584558 .016002341 .008921812 .010236046 .010326799 .009985939 .010706581 .01540841 .011336271 .01285533 .021928284 .015842749 .00735747 .010816324 .016563475 .015062229 ACETOACETYL-COA .008904697 .008970614 .009052976 .00901687 .00903461 .009480267 .009234711 .00938678 .009104327 .00909944 .009018288 .008885126 .009439027 .00907401 .008924986 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type D-ERYTHROSE 4-PHOSPHATE 122357 BETA-NAD+ 15938971 COENZYME II 15938972 SEDOHEPTULOSE 7-PHOSPHATE 165007 D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE) 21125049 CITRIC ACID 311 NADH 439153 FAD 46906035 MALIC ACID 525 ADENOSINE TRIPHOSPHATE 5957 ROBISON ESTER 5958 SUCROSE 5988 ADENOSINE 5'-DIPHOSPHATE 6022 ADENOSINE 5'-MONOPHOSPHATE 6083 GLYCERATE 3-PHOSPHATE 724 RIBOSE PHOSPHATE 77982 6-PHOSPHOGLUCONIC ACID 91493 ACETOACETYL-COA 92153 METABOLITES_END #END