#METABOLOMICS WORKBENCH hormel101_20170810_132003_mwtab.txt DATATRACK_ID:1205 STUDY_ID:ST000869 ANALYSIS_ID:AN001400 PROJECT_ID:PR000575
VERSION             	1
CREATED_ON             	August 28, 2017, 10:41 am
#PROJECT
PR:PROJECT_TITLE                 	Mayo Pilot and Feasiblity: Targeting Myelin Metabolism of Kallikrein 6 Signals
PR:PROJECT_TITLE                 	through PAR1 and PAR2 to Enchance Recovery of Function after SCI
PR:PROJECT_SUMMARY               	CNS trauma generates a proteolytic imbalance contributing to secondary injury,
PR:PROJECT_SUMMARY               	including axonopathy and neuron degeneration. Kallikrein 6 (Klk6) is a serine
PR:PROJECT_SUMMARY               	protease implicated in neurodegeneration and here we investigate the role of
PR:PROJECT_SUMMARY               	protease activated receptors 1 (PAR1) and PAR2 in mediating these effects in
PR:PROJECT_SUMMARY               	mice with spinal cord injury (SCI). This project conducts untargeted Profiling
PR:PROJECT_SUMMARY               	(unbiased metabolomics assay) and targeted lipid analysis. The lipid assays are
PR:PROJECT_SUMMARY               	1) free fatty acid composition of lipids; 2) free fatty acid panel; 3)
PR:PROJECT_SUMMARY               	cholesterol concentration (free and bound); 4) Ceramides, including galactosyl
PR:PROJECT_SUMMARY               	and glucosyl; 5) sphingomyelin.
PR:INSTITUTE                     	Mayo Clinic
PR:LAST_NAME                     	Scarisbrick
PR:FIRST_NAME                    	Isobel
PR:ADDRESS                       	200 First Street SW, Rochester, MN 55905
PR:EMAIL                         	scarisbrick.isobel@mayo.edu
PR:PHONE                         	507-284-0124
#STUDY
ST:STUDY_TITLE                   	Large Untargeted Profiling in Kallikrein 6 Mice after SCI
ST:STUDY_SUMMARY                 	Large untargeted profiling mouse myelin of Kallikrein 6 Signals through PAR1 and
ST:STUDY_SUMMARY                 	PAR2 to Enchance Recovery of Function after SCI. The samples submitted are
ST:STUDY_SUMMARY                 	purified myelin preparations from the postnatal day 21, 60, or 90 mouse spinal
ST:STUDY_SUMMARY                 	cord (SC). There are 12 samples total in Project 3, n=3 for K6+/+ or K6-/- at
ST:STUDY_SUMMARY                 	either P21 or P90.
ST:INSTITUTE                     	Mayo Clinic
ST:LAST_NAME                     	Scarisbrick
ST:FIRST_NAME                    	Isobel
ST:ADDRESS                       	200 First Street SW, Rochester, MN 55905
ST:EMAIL                         	scarisbrick.isobel@mayo.edu
ST:PHONE                         	507-284-0124
#SUBJECT
SU:SUBJECT_TYPE                  	Mouse
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	MS6020-1	nC18-8jun16-001-r001_1	Group:K6-/- | Time point:P21 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-1	nC18-8jun16-001-r002_1	Group:K6-/- | Time point:P21 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-2	nC18-8jun16-002-r001_1	Group:K6-/- | Time point:P90 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-2	nC18-8jun16-002-r002_1	Group:K6-/- | Time point:P90 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-3	nC18-8jun16-003-r001_1	Group:K6+/+ | Time point:P21 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-3	nC18-8jun16-003-r002_1	Group:K6+/+ | Time point:P21 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-4	nC18-8jun16-004-r001_1	Group:K6+/+ | Time point:P21 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-4	nC18-8jun16-004-r002_1	Group:K6+/+ | Time point:P21 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-5	nC18-8jun16-005-r001_1	Group:K6-/- | Time point:P21 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-5	nC18-8jun16-005-r002_1	Group:K6-/- | Time point:P21 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-6	nC18-8jun16-006-r001_1	Group:K6-/- | Time point:P90 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-6	nC18-8jun16-006-r002_1	Group:K6-/- | Time point:P90 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-7	nC18-8jun16-007-r001_1	Group:K6+/+ | Time point:P90 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-7	nC18-8jun16-007-r002_1	Group:K6+/+ | Time point:P90 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-8	nC18-8jun16-008-r001_1	Group:K6+/+ | Time point:P90 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-8	nC18-8jun16-008-r002_1	Group:K6+/+ | Time point:P90 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-9	nC18-8jun16-009-r001_1	Group:K6-/- | Time point:P21 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-9	nC18-8jun16-009-r002_1	Group:K6-/- | Time point:P21 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-10	nC18-8jun16-010-r001_1	Group:K6+/+ | Time point:P21 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-10	nC18-8jun16-010-r002_1	Group:K6+/+ | Time point:P21 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-11	nC18-8jun16-011-r001_1	Group:K6+/+ | Time point:P90 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-11	nC18-8jun16-011-r002_1	Group:K6+/+ | Time point:P90 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-12	nC18-8jun16-012-r001_1	Group:K6-/- | Time point:P90 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-12	nC18-8jun16-012-r002_1	Group:K6-/- | Time point:P90 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-13	nC18-8jun16-013-r001_1	Group:K6-/- | Time point:P90 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-13	nC18-8jun16-013-r002_1	Group:K6-/- | Time point:P90 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-14	nC18-8jun16-014-r001_1	Group:K6+/+ | Time point:P90 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-14	nC18-8jun16-014-r002_1	Group:K6+/+ | Time point:P90 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-15	nC18-8jun16-015-r001_1	Group:K6+/+ | Time point:P21 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-15	nC18-8jun16-015-r002_1	Group:K6+/+ | Time point:P21 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-16	nC18-8jun16-016-r001_1	Group:K6-/- | Time point:P21 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-16	nC18-8jun16-016-r002_1	Group:K6-/- | Time point:P21 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-17	nC18-8jun16-017-r001_1	Group:K6-/- | Time point:P90 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-17	nC18-8jun16-017-r002_1	Group:K6-/- | Time point:P90 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-18	nC18-8jun16-018-r001_1	Group:K6+/+ | Time point:P21 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-18	nC18-8jun16-018-r002_1	Group:K6+/+ | Time point:P21 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-19	nC18-8jun16-019-r001_1	Group:K6+/+ | Time point:P90 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-19	nC18-8jun16-019-r002_1	Group:K6+/+ | Time point:P90 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-20	nC18-8jun16-020-r001_1	Group:K6-/- | Time point:P21 | rep:1	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-20	nC18-8jun16-020-r002_1	Group:K6-/- | Time point:P21 | rep:2	type=NC18
SUBJECT_SAMPLE_FACTORS           	MS6020-1	pC18-7jun16-001-r001	Group:K6-/- | Time point:P21 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-1	pC18-7jun16-001-r002	Group:K6-/- | Time point:P21 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-2	pC18-7jun16-002-r001	Group:K6-/- | Time point:P90 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-2	pC18-7jun16-002-r002	Group:K6-/- | Time point:P90 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-3	pC18-7jun16-003-r001	Group:K6+/+ | Time point:P21 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-3	pC18-7jun16-003-r002	Group:K6+/+ | Time point:P21 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-4	pC18-7jun16-004-r001	Group:K6+/+ | Time point:P21 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-4	pC18-7jun16-004-r002	Group:K6+/+ | Time point:P21 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-5	pC18-7jun16-005-r001	Group:K6-/- | Time point:P21 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-5	pC18-7jun16-005-r002	Group:K6-/- | Time point:P21 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-6	pC18-7jun16-006-r001	Group:K6-/- | Time point:P90 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-6	pC18-7jun16-006-r002	Group:K6-/- | Time point:P90 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-7	pC18-7jun16-007-r001	Group:K6+/+ | Time point:P90 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-7	pC18-7jun16-007-r002	Group:K6+/+ | Time point:P90 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-8	pC18-7jun16-008-r001	Group:K6+/+ | Time point:P90 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-8	pC18-7jun16-008-r002	Group:K6+/+ | Time point:P90 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-9	pC18-7jun16-009-r001	Group:K6-/- | Time point:P21 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-9	pC18-7jun16-009-r002	Group:K6-/- | Time point:P21 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-10	pC18-7jun16-010-r001	Group:K6+/+ | Time point:P21 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-10	pC18-7jun16-010-r002	Group:K6+/+ | Time point:P21 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-11	pC18-7jun16-011-r001	Group:K6+/+ | Time point:P90 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-11	pC18-7jun16-011-r002	Group:K6+/+ | Time point:P90 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-12	pC18-7jun16-012-r001	Group:K6-/- | Time point:P90 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-12	pC18-7jun16-012-r002	Group:K6-/- | Time point:P90 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-13	pC18-7jun16-013-r001	Group:K6-/- | Time point:P90 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-13	pC18-7jun16-013-r002	Group:K6-/- | Time point:P90 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-14	pC18-7jun16-014-r001	Group:K6+/+ | Time point:P90 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-14	pC18-7jun16-014-r002	Group:K6+/+ | Time point:P90 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-15	pC18-7jun16-015-r001	Group:K6+/+ | Time point:P21 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-15	pC18-7jun16-015-r002	Group:K6+/+ | Time point:P21 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-16	pC18-7jun16-016-r001	Group:K6-/- | Time point:P21 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-16	pC18-7jun16-016-r002	Group:K6-/- | Time point:P21 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-17	pC18-7jun16-017-r001	Group:K6-/- | Time point:P90 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-17	pC18-7jun16-017-r002	Group:K6-/- | Time point:P90 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-18	pC18-7jun16-018-r001	Group:K6+/+ | Time point:P21 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-18	pC18-7jun16-018-r002	Group:K6+/+ | Time point:P21 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-19	pC18-7jun16-019-r001	Group:K6+/+ | Time point:P90 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-19	pC18-7jun16-019-r002	Group:K6+/+ | Time point:P90 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-20	pC18-7jun16-020-r001	Group:K6-/- | Time point:P21 | rep:1	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-20	pC18-7jun16-020-r002	Group:K6-/- | Time point:P21 | rep:2	type=PC18
SUBJECT_SAMPLE_FACTORS           	MS6020-1	nhilic-9jun16-001-r001	Group:K6-/- | Time point:P21 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-1	nhilic-9jun16-001-r002	Group:K6-/- | Time point:P21 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-2	nhilic-9jun16-002-r001	Group:K6-/- | Time point:P90 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-2	nhilic-9jun16-002-r002	Group:K6-/- | Time point:P90 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-3	nhilic-9jun16-003-r001	Group:K6+/+ | Time point:P21 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-3	nhilic-9jun16-003-r002	Group:K6+/+ | Time point:P21 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-4	nhilic-9jun16-004-r001	Group:K6+/+ | Time point:P21 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-4	nhilic-9jun16-004-r002	Group:K6+/+ | Time point:P21 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-5	nhilic-9jun16-005-r001	Group:K6-/- | Time point:P21 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-5	nhilic-9jun16-005-r002	Group:K6-/- | Time point:P21 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-6	nhilic-9jun16-006-r001	Group:K6-/- | Time point:P90 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-6	nhilic-9jun16-006-r002	Group:K6-/- | Time point:P90 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-7	nhilic-9jun16-007-r001	Group:K6+/+ | Time point:P90 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-7	nhilic-9jun16-007-r002	Group:K6+/+ | Time point:P90 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-8	nhilic-9jun16-008-r001	Group:K6+/+ | Time point:P90 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-8	nhilic-9jun16-008-r002	Group:K6+/+ | Time point:P90 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-9	nhilic-9jun16-009-r001	Group:K6-/- | Time point:P21 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-9	nhilic-9jun16-009-r002	Group:K6-/- | Time point:P21 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-10	nhilic-9jun16-010-r001	Group:K6+/+ | Time point:P21 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-10	nhilic-9jun16-010-r002	Group:K6+/+ | Time point:P21 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-11	nhilic-9jun16-011-r001	Group:K6+/+ | Time point:P90 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-11	nhilic-9jun16-011-r002	Group:K6+/+ | Time point:P90 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-12	nhilic-9jun16-012-r001	Group:K6-/- | Time point:P90 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-12	nhilic-9jun16-012-r002	Group:K6-/- | Time point:P90 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-13	nhilic-9jun16-013-r001	Group:K6-/- | Time point:P90 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-13	nhilic-9jun16-013-r002	Group:K6-/- | Time point:P90 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-14	nhilic-9jun16-014-r001	Group:K6+/+ | Time point:P90 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-14	nhilic-9jun16-014-r002	Group:K6+/+ | Time point:P90 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-15	nhilic-9jun16-015-r001	Group:K6+/+ | Time point:P21 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-15	nhilic-9jun16-015-r002	Group:K6+/+ | Time point:P21 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-16	nhilic-9jun16-016-r001	Group:K6-/- | Time point:P21 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-16	nhilic-9jun16-016-r002	Group:K6-/- | Time point:P21 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-17	nhilic-9jun16-017-r001	Group:K6-/- | Time point:P90 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-17	nhilic-9jun16-017-r002	Group:K6-/- | Time point:P90 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-18	nhilic-9jun16-018-r001	Group:K6+/+ | Time point:P21 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-18	nhilic-9jun16-018-r002	Group:K6+/+ | Time point:P21 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-19	nhilic-9jun16-019-r001	Group:K6+/+ | Time point:P90 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-19	nhilic-9jun16-019-r002	Group:K6+/+ | Time point:P90 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-20	nhilic-9jun16-020-r001	Group:K6-/- | Time point:P21 | rep:1	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-20	nhilic-9jun16-020-r002	Group:K6-/- | Time point:P21 | rep:2	type=NHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-1	philic-7jun16-001-r001	Group:K6-/- | Time point:P21 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-1	philic-7jun16-001-r002	Group:K6-/- | Time point:P21 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-2	philic-7jun16-002-r001	Group:K6-/- | Time point:P90 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-2	philic-7jun16-002-r002	Group:K6-/- | Time point:P90 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-3	philic-7jun16-003-r001	Group:K6+/+ | Time point:P21 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-3	philic-7jun16-003-r002	Group:K6+/+ | Time point:P21 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-4	philic-7jun16-004-r001	Group:K6+/+ | Time point:P21 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-4	philic-7jun16-004-r002	Group:K6+/+ | Time point:P21 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-5	philic-7jun16-005-r001	Group:K6-/- | Time point:P21 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-5	philic-7jun16-005-r002	Group:K6-/- | Time point:P21 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-6	philic-7jun16-006-r001	Group:K6-/- | Time point:P90 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-6	philic-7jun16-006-r002	Group:K6-/- | Time point:P90 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-7	philic-7jun16-007-r001	Group:K6+/+ | Time point:P90 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-7	philic-7jun16-007-r002	Group:K6+/+ | Time point:P90 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-8	philic-7jun16-008-r001	Group:K6+/+ | Time point:P90 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-8	philic-7jun16-008-r002	Group:K6+/+ | Time point:P90 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-9	philic-7jun16-009-r001	Group:K6-/- | Time point:P21 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-9	philic-7jun16-009-r002	Group:K6-/- | Time point:P21 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-10	philic-7jun16-010-r001	Group:K6+/+ | Time point:P21 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-10	philic-7jun16-010-r002	Group:K6+/+ | Time point:P21 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-11	philic-7jun16-011-r001	Group:K6+/+ | Time point:P90 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-11	philic-7jun16-011-r002	Group:K6+/+ | Time point:P90 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-12	philic-7jun16-012-r001	Group:K6-/- | Time point:P90 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-12	philic-7jun16-012-r002	Group:K6-/- | Time point:P90 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-13	philic-7jun16-013-r001	Group:K6-/- | Time point:P90 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-13	philic-7jun16-013-r002	Group:K6-/- | Time point:P90 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-14	philic-7jun16-014-r001	Group:K6+/+ | Time point:P90 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-14	philic-7jun16-014-r002	Group:K6+/+ | Time point:P90 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-15	philic-7jun16-015-r001	Group:K6+/+ | Time point:P21 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-15	philic-7jun16-015-r002	Group:K6+/+ | Time point:P21 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-16	philic-7jun16-016-r001	Group:K6-/- | Time point:P21 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-16	philic-7jun16-016-r002	Group:K6-/- | Time point:P21 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-17	philic-7jun16-017-r001	Group:K6-/- | Time point:P90 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-17	philic-7jun16-017-r002	Group:K6-/- | Time point:P90 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-18	philic-7jun16-018-r001	Group:K6+/+ | Time point:P21 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-18	philic-7jun16-018-r002	Group:K6+/+ | Time point:P21 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-19	philic-7jun16-019-r001	Group:K6+/+ | Time point:P90 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-19	philic-7jun16-019-r002	Group:K6+/+ | Time point:P90 | rep:2	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-20	philic-7jun16-020-r001	Group:K6-/- | Time point:P21 | rep:1	type=PHILIC
SUBJECT_SAMPLE_FACTORS           	MS6020-20	philic-7jun16-020-r002	Group:K6-/- | Time point:P21 | rep:2	type=PHILIC
#COLLECTION
CO:COLLECTION_SUMMARY            	Tissue is from adult mouse spinal cord (SC). We are submitting these samples for
CO:COLLECTION_SUMMARY            	Untargeted Profiling (unbiased metabolomics assay) and for lipid analysis. The
CO:COLLECTION_SUMMARY            	lipid assays we request are 1) free fatty acid composition of lipids; 2) free
CO:COLLECTION_SUMMARY            	fatty acid panel; 3) cholesterol concentration (free and bound); 4) Ceramides,
CO:COLLECTION_SUMMARY            	including galactosyl and glucosyl; 5) sphingomyelin. The Untargeted profiling is
CO:COLLECTION_SUMMARY            	our top priority, followed by the lipid assays as listed. All samples were snap
CO:COLLECTION_SUMMARY            	frozen at the point of harvest and approximate weights are provided. The samples
CO:COLLECTION_SUMMARY            	are submitted as intact pieces of tissue. There are two different genotypes
CO:COLLECTION_SUMMARY            	(K6+/+ and K6-/-) and 20 samples total, n=5 for each group that includes
CO:COLLECTION_SUMMARY            	P21(K6+/+); P90 (K6+/+); P21 (K6-/-); and P90 (K6-/-). We would like to make
CO:COLLECTION_SUMMARY            	comparisons across the 4 groups.
#TREATMENT
TR:TREATMENT_SUMMARY             	A 3g Clip produces moderate SCI including demyelination and clinical impairment
TR:TREATMENT_SUMMARY             	and we recently published a detailed methodology. At 1 week after injury, the 3g
TR:TREATMENT_SUMMARY             	injured mice are expected to have an average Basso Mouse Scale score (BMS)=5 on
TR:TREATMENT_SUMMARY             	a 9 point scale such that they have frequent plantar stepping with no or some
TR:TREATMENT_SUMMARY             	coordination. This level of impairment was chosen to provide a sufficient window
TR:TREATMENT_SUMMARY             	to observe recovery.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	"large scale profiling of mouse spinal cord Lipids will be quantified in myelin
SP:SAMPLEPREP_SUMMARY            	isolated in high yield and purity by subcellular fractionation from the
SP:SAMPLEPREP_SUMMARY            	lumbosacral spinal cord. While there are no absolutely ‘myelin-specific’
SP:SAMPLEPREP_SUMMARY            	lipids, galactocerebroside is the most typical of myelin in the adult nervous
SP:SAMPLEPREP_SUMMARY            	system being directly proportional to the amount of myelin. Sulfatide is another
SP:SAMPLEPREP_SUMMARY            	galactolipid enriched in myelin. Together with cholesterol, these form 78% of
SP:SAMPLEPREP_SUMMARY            	the total amount of lipid in the myelin membrane and each will be quantified
SP:SAMPLEPREP_SUMMARY            	using LC/MS/MS. A highly sensitive assay for galactocerebroside was recently
SP:SAMPLEPREP_SUMMARY            	established by the Mayo Metabolomics Core and can be implemented immediately.
SP:SAMPLEPREP_SUMMARY            	The LC/MS/MS panel for free fatty acids, including the very long chain fatty
SP:SAMPLEPREP_SUMMARY            	acids found in myelin is also routinely performed by the Core. Cholesterol will
SP:SAMPLEPREP_SUMMARY            	be quantified using an NMR-based approach by the Mayo Dept. of Laboratory
SP:SAMPLEPREP_SUMMARY            	Medicine Clinical Core. Additionally, we have a plan in place with the
SP:SAMPLEPREP_SUMMARY            	Metabolomics Core to develop LC/MS/MS assays for sulfatide and sphingomyelin
SP:SAMPLEPREP_SUMMARY            	during the Pilot proposal. Having quantitative assays for each of these key
SP:SAMPLEPREP_SUMMARY            	myelin lipids will facilitate our goal to comprehensively profile myelin lipid
SP:SAMPLEPREP_SUMMARY            	metabolism and will form foundational assays for a future NIH grant focused on
SP:SAMPLEPREP_SUMMARY            	myelin metabolism."
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity
CH:COLUMN_NAME                   	Waters Acquity BEH Amide (150 x 2.1mm, 1.7um)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent 6550 QTOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_RESULTS_FILE               	ST000869_AN001400_Results.txt	UNITS:intensity
#END