#METABOLOMICS WORKBENCH michaelsa93_20170830_131510_mwtab.txt DATATRACK_ID:1230 STUDY_ID:ST000873 ANALYSIS_ID:AN001478 PROJECT_ID:PR000604
VERSION             	1
CREATED_ON             	January 3, 2018, 4:03 pm
#PROJECT
PR:PROJECT_TITLE                 	Maternal Hypoxemia and oxidative stress in the fetus, newborn, and adult.
PR:PROJECT_SUMMARY               	Gestational hypoxia presents a significant stress to an unborn fetus that can
PR:PROJECT_SUMMARY               	lead to significant complications related to fetal growth restriction and
PR:PROJECT_SUMMARY               	resulting in diseases in the newborn as well as those manifesting later in life.
PR:PROJECT_SUMMARY               	Recent evidence indicates that inflammation and oxidative stress are
PR:PROJECT_SUMMARY               	contributing factors to hypoxia-related diseases. The Center for Perinatal
PR:PROJECT_SUMMARY               	Biology at Loma Linda University has studied gestational chronic hypoxia in a
PR:PROJECT_SUMMARY               	sheep model for over 20 years to study dysfunction of vascular and nonvascular
PR:PROJECT_SUMMARY               	tissues derived from mothers, fetuses and offspring. In this project we are
PR:PROJECT_SUMMARY               	attempting to use metabolomics to assess metabolic dysregulation in vascular
PR:PROJECT_SUMMARY               	tissues along with markers of oxidative stress and inflammation in the mother
PR:PROJECT_SUMMARY               	and offspring to determine the extent of dysregulation due to chronic hypoxia.
PR:PROJECT_SUMMARY               	Untargeted metabolomics analysis focused on sheep plasma and arteries from the
PR:PROJECT_SUMMARY               	lung, resistance arteries in the brain, uterine arteries, and cultured human
PR:PROJECT_SUMMARY               	myocytes will be used to explore markers of glucose and lipid metabolism
PR:PROJECT_SUMMARY               	disruption. Targeted analyses of oxylipins and endocannabinoids will be used on
PR:PROJECT_SUMMARY               	the same samples to explore markers of oxidative stress and inflammation, which
PR:PROJECT_SUMMARY               	should be increased during hypoxia. This study should delineate pathways and
PR:PROJECT_SUMMARY               	biomarkers that help explain how hypoxia leads to the development of neonatal as
PR:PROJECT_SUMMARY               	well as adult-onset diseases associated with chronic hypoxia that are
PR:PROJECT_SUMMARY               	inter-related with fetal growth restriction.
PR:INSTITUTE                     	University of California, Davis
PR:DEPARTMENT                    	Genome and Biomedical Sciences Facility
PR:LABORATORY                    	WCMC Metabolomics Core
PR:LAST_NAME                     	Fiehn
PR:FIRST_NAME                    	Oliver
PR:ADDRESS                       	1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis,
PR:ADDRESS                       	CA 95616
PR:EMAIL                         	ofiehn@ucdavis.edu
PR:PHONE                         	(530) 754-8258
PR:FUNDING_SOURCE                	NIH U24DK097154
#STUDY
ST:STUDY_TITLE                   	Maternal Hypoxemia and oxidative stress in the fetus, newborn, and adult.
ST:STUDY_TITLE                   	exercise training for peripheral artery disease (part II)
ST:STUDY_TYPE                    	Disease model
ST:STUDY_SUMMARY                 	Gestational hypoxia presents a significant stress to an unborn fetus that can
ST:STUDY_SUMMARY                 	lead to significant complications related to fetal growth restriction and
ST:STUDY_SUMMARY                 	resulting in diseases in the newborn as well as those manifesting later in life.
ST:STUDY_SUMMARY                 	Recent evidence indicates that inflammation and oxidative stress are
ST:STUDY_SUMMARY                 	contributing factors to hypoxia-related diseases. The Center for Perinatal
ST:STUDY_SUMMARY                 	Biology at Loma Linda University has studied gestational chronic hypoxia in a
ST:STUDY_SUMMARY                 	sheep model for over 20 years to study dysfunction of vascular and nonvascular
ST:STUDY_SUMMARY                 	tissues derived from mothers, fetuses and offspring. In this project we are
ST:STUDY_SUMMARY                 	attempting to use metabolomics to assess metabolic dysregulation in vascular
ST:STUDY_SUMMARY                 	tissues along with markers of oxidative stress and inflammation in the mother
ST:STUDY_SUMMARY                 	and offspring to determine the extent of dysregulation due to chronic hypoxia.
ST:STUDY_SUMMARY                 	Untargeted metabolomics analysis focused on sheep plasma and arteries from the
ST:STUDY_SUMMARY                 	lung, resistance arteries in the brain, uterine arteries, and cultured human
ST:STUDY_SUMMARY                 	myocytes will be used to explore markers of glucose and lipid metabolism
ST:STUDY_SUMMARY                 	disruption. Targeted analyses of oxylipins and endocannabinoids will be used on
ST:STUDY_SUMMARY                 	the same samples to explore markers of oxidative stress and inflammation, which
ST:STUDY_SUMMARY                 	should be increased during hypoxia. This study should delineate pathways and
ST:STUDY_SUMMARY                 	biomarkers that help explain how hypoxia leads to the development of neonatal as
ST:STUDY_SUMMARY                 	well as adult-onset diseases associated with chronic hypoxia that are
ST:STUDY_SUMMARY                 	inter-related with fetal growth restriction.
ST:INSTITUTE                     	USDA
ST:DEPARTMENT                    	Obesity and metabolism research unit
ST:LABORATORY                    	Newman's Lab
ST:LAST_NAME                     	Newman
ST:FIRST_NAME                    	John
ST:ADDRESS                       	430 West Health Sciences Dr. Davis, Ca, 95616
ST:EMAIL                         	John.Newman@ars.usda.gov
ST:PHONE                         	(530) 752-1009
#SUBJECT
SU:SUBJECT_TYPE                  	Sheep
SU:SUBJECT_SPECIES               	Ovis aries
SU:TAXONOMY_ID                   	9940
SU:GENDER                        	Both
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	S41	Tissue type:Adipose | Treatment group:Normoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S42	Tissue type:Adipose | Treatment group:Normoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S43	Tissue type:Adipose | Treatment group:Normoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S44	Tissue type:Adipose | Treatment group:Normoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S45	Tissue type:Adipose | Treatment group:Normoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S46	Tissue type:Adipose | Treatment group:Normoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S54	Tissue type:Adipose | Treatment group:Normoxic | Life stage:New Born	
SUBJECT_SAMPLE_FACTORS           	-	S55	Tissue type:Adipose | Treatment group:Normoxic | Life stage:New Born	
SUBJECT_SAMPLE_FACTORS           	-	S56	Tissue type:Adipose | Treatment group:Normoxic | Life stage:New Born	
SUBJECT_SAMPLE_FACTORS           	-	S57	Tissue type:Adipose | Treatment group:Normoxic | Life stage:New Born	
SUBJECT_SAMPLE_FACTORS           	-	S58	Tissue type:Adipose | Treatment group:Normoxic | Life stage:New Born	
SUBJECT_SAMPLE_FACTORS           	-	S47	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S48	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S49	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S50	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S51	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S52	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S53	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S59	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:New Born	
SUBJECT_SAMPLE_FACTORS           	-	S60	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:New Born	
SUBJECT_SAMPLE_FACTORS           	-	S61	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:New Born	
SUBJECT_SAMPLE_FACTORS           	-	S62	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:New Born	
SUBJECT_SAMPLE_FACTORS           	-	S63	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:New Born	
SUBJECT_SAMPLE_FACTORS           	-	S64	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:New Born	
SUBJECT_SAMPLE_FACTORS           	-	S36	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S37	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S38	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S39	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S40	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S31	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S32	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S33	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S34	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S35	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S26	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S27	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S28	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S29	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S30	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S21	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S23	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S24	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S25	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S107	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S89	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S91	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S93	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S104	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S105	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S106	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S71	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:New Born	
SUBJECT_SAMPLE_FACTORS           	-	S72	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:New Born	
SUBJECT_SAMPLE_FACTORS           	-	S95	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:New Born	
SUBJECT_SAMPLE_FACTORS           	-	S79	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S80	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S81	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S82	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S101	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S102	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S83	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S84	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S85	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S87	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S88	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S103	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Fetal	
SUBJECT_SAMPLE_FACTORS           	-	S74	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:New Born	
SUBJECT_SAMPLE_FACTORS           	-	S96	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:New Born	
SUBJECT_SAMPLE_FACTORS           	-	S97	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:New Born	
SUBJECT_SAMPLE_FACTORS           	-	S65	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S66	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S67	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S68	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S98	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S99	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S100	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S1	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S2	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S3	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S4	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S5	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S6	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S7	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S8	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S9	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S10	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S11	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S12	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S13	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S14	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S15	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S16	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S17	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S18	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S19	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult pregnant	
SUBJECT_SAMPLE_FACTORS           	-	S20	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult pregnant	
#COLLECTION
CO:COLLECTION_SUMMARY            	Part of the routinely flash frozen samples from the Loma Linda University School
CO:COLLECTION_SUMMARY            	of Medicine, Center for Perinatal Biology.
CO:SAMPLE_TYPE                   	Tissue
CO:TISSUE_CELL_IDENTIFICATION    	Adipose tissue, Carotid, pulmonary arteries, uterine arteries
#TREATMENT
TR:TREATMENT_SUMMARY             	To induce chronic hypoxia, pregnant and non-pregnant ewes were transported to
TR:TREATMENT_SUMMARY             	the White Mountain Research Station that is owned and operated by the University
TR:TREATMENT_SUMMARY             	of California. Animals were housed at the Barcroft Research Station (3800 m) for
TR:TREATMENT_SUMMARY             	100+ days prior to having the pregnant or non-pregnant ewes or 2 week old
TR:TREATMENT_SUMMARY             	newborn lambs transported back to Loma Linda for study.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	After sample randomization, weighed tissue (around 10mg for adipose and 50mg for
SP:SAMPLEPREP_SUMMARY            	arteries) in 2 mL polypropylene Eppendorf tube was enriched with deuterated
SP:SAMPLEPREP_SUMMARY            	surrogates in 20µL methanol (Tables S1 and S2 from Agrawal, K., L.A. Hassoun,
SP:SAMPLEPREP_SUMMARY            	N. Foolad, T.L. Pedersen, R.K. Sivamani, J.W. Newman. 2017. Sweat lipid mediator
SP:SAMPLEPREP_SUMMARY            	profiling: a non-invasive approach for cutaneous research. J. Lipid Res.
SP:SAMPLEPREP_SUMMARY            	58:188–195 [EPub: Nov 7, 2016]. doi: 10.1194/jlr.M071738) and 5 μl of
SP:SAMPLEPREP_SUMMARY            	BHT/EDTA in 1:1 methanol/water (v/v). A total of 400 μl 1-cyclohexyl uredio,
SP:SAMPLEPREP_SUMMARY            	3-dodecanoic acid / 1-phenyl ureido, 3-hexanoic acid (CUDA / PUHA) in 1:1
SP:SAMPLEPREP_SUMMARY            	methanol/acetonitrile (v/v) was added, the sample ground with 3mm stainless
SP:SAMPLEPREP_SUMMARY            	steel beads (two for adipose tissue and 3 for blood vessel tissue) and agitation
SP:SAMPLEPREP_SUMMARY            	for 6 min at 1500RPM. Protein precipitate and debris were removed by
SP:SAMPLEPREP_SUMMARY            	centrifugation (10 min, 10,000 RCF, 6 °C). The supernatant was filtered by
SP:SAMPLEPREP_SUMMARY            	centrifugation through 1 µm PVDF membranes (Millipore, Billerica, MA) at 6 °C
SP:SAMPLEPREP_SUMMARY            	and 4,500 RCF for 3 min. The filtrate was stored in glass vials at -20 °C until
SP:SAMPLEPREP_SUMMARY            	UPLC-MS/MS analysis.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Waters Acquity
CH:COLUMN_NAME                   	Aquity C18 BEH 1.7µm 100mm x 2.1mm column
CH:FLOW_GRADIENT                 	See protocol/methods file
CH:FLOW_RATE                     	0.25 mL/min
CH:COLUMN_TEMPERATURE            	60 °C
CH:SOLVENT_A                     	0.1% acetic acid
CH:SOLVENT_B                     	90% ACN / 10% IPA
CH:INTERNAL_STANDARD             	See protocol/methods file
CH:RETENTION_TIME                	See protocol/methods file
CH:SAMPLE_INJECTION              	5 µL
CH:ANALYTICAL_TIME               	20 min
CH:WEAK_WASH_SOLVENT_NAME        	20% methanol, 10% isopropanol
CH:WEAK_WASH_VOLUME              	600 µL
CH:STRONG_WASH_SOLVENT_NAME      	50:50 Acetonitrile:Methanol
CH:STRONG_WASH_VOLUME            	600 µL
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:MS_COMMENTS                   	-
MS:INSTRUMENT_NAME               	ABI Sciex 6500 QTrap
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	Concentration (nM)
MS_METABOLITE_DATA_START
Samples	S41	S42	S43	S44	S45	S46	S54	S55	S56	S57	S58	S47	S48	S49	S50	S51	S52	S53	S59	S60	S61	S62	S63	S64	S36	S37	S38	S39	S40	S31	S32	S33	S34	S35	S26	S27	S28	S29	S30	S21	S23	S24	S25	S107	S89	S91	S93	S104	S105	S106	S71	S72	S95	S79	S80	S81	S82	S101	S102	S83	S84	S85	S87	S88	S103	S74	S96	S97	S65	S66	S67	S68	S98	S99	S100	S1	S2	S3	S4	S5	S6	S7	S8	S9	S10	S11	S12	S13	S14	S15	S16	S17	S18	S19	S20
Factors	Tissue type:Adipose | Treatment group:Normoxic | Life stage:Fetal	Tissue type:Adipose | Treatment group:Normoxic | Life stage:Fetal	Tissue type:Adipose | Treatment group:Normoxic | Life stage:Fetal	Tissue type:Adipose | Treatment group:Normoxic | Life stage:Fetal	Tissue type:Adipose | Treatment group:Normoxic | Life stage:Fetal	Tissue type:Adipose | Treatment group:Normoxic | Life stage:Fetal	Tissue type:Adipose | Treatment group:Normoxic | Life stage:New Born	Tissue type:Adipose | Treatment group:Normoxic | Life stage:New Born	Tissue type:Adipose | Treatment group:Normoxic | Life stage:New Born	Tissue type:Adipose | Treatment group:Normoxic | Life stage:New Born	Tissue type:Adipose | Treatment group:Normoxic | Life stage:New Born	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:New Born	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:New Born	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:New Born	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:New Born	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:New Born	Tissue type:Adipose | Treatment group:Hypoxic | Life stage:New Born	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Fetal	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Fetal	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Fetal	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Fetal	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Fetal	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Adult non-pregnant	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Adult non-pregnant	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Adult non-pregnant	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Adult non-pregnant	Tissue type:Carotid | Treatment group:Normoxic | Life stage:Adult non-pregnant	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Adult non-pregnant	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Adult non-pregnant	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Adult non-pregnant	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Adult non-pregnant	Tissue type:Carotid | Treatment group:Hypoxic | Life stage:Adult non-pregnant	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Fetal	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Fetal	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Fetal	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Fetal	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Fetal	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Fetal	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:New Born	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:New Born	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:New Born	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	Tissue type:Pulmonary Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Fetal	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:New Born	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:New Born	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:New Born	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	Tissue type:Pulmonary Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult non-pregnant	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult pregnant	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult pregnant	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult pregnant	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult pregnant	Tissue type:Uterine Arteries | Treatment group:Normoxic | Life stage:Adult pregnant	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult non-pregnant	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult pregnant	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult pregnant	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult pregnant	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult pregnant	Tissue type:Uterine Arteries | Treatment group:Hypoxic | Life stage:Adult pregnant
6-keto PGF1a	5.75	7.2	3.35	17.4	1.34	11.5	44.6	12.8	35.8	7.93	8.07	13.2	12.8	7.77	9.11	13.1	6.97	5.96	1.4	19.4	12.9	12.9	11.3	7.19	104	412	139	207	255	1460	600	297	137	38.1	392	369	371	282	444	72.5	66.7	66.2	587	490	431	83.4	269	510	1030	1110	2760	2330	9090	350	1360	840	300	1830	3170	80.4	238	137	287	273	2830	1180	7980	4200	102	272	35.2	18.3	3210	3090	2820	259	294	353	201	68.5	301	231	316	672	204	690	267	390	213	525	810	329	210	230	72
TXB2	4.4	0.862	2.57	8.87	1.52	1.97	3.04	1.73	6.6	4.37	2.21	5	3.9	3.08	3.49	2.31	2.1	5.81	1.66	3.36	3.57	1.91	1	1.36	7.56	3.97	5.27	1.31	7.39	30.6	13	5.81	2.9	1.29	1.61	16	1.44	4.12	1.37	1.92	1.8	2.09	10.8	19.5	5.91	0.933	12.1	3.04	3.6	4.1	263	327	714	11.4	51.1	30.7	29.9	63.1	255	11.6	6.12	2.08	4.61	6.45	15.7	69	215	160	45.2	76.8	57.9	39.3	268	707	68.9	10.6	11.7	8.74	2.96	2.13	3.33	3	2.52	6.03	2.19	16.2	18.6	14.7	5.43	23.2	16.2	4.93	2.83	4.21	1.59
11-HETE	30.6	5.62	20.5	27.2	9.02	24.1	5.87	7.32	6.67	3.42	2.67	23.6	11.9	13.9	29.3	9.84	28.8	22.4	4.05	9.87	18.1	4.79	0.499	0.961	26.6	31.4	47.1	17.2	20.2	478	78.1	8.8	6.09	3.24	36.8	45.8	47.2	50.4	47.7	8.7	5.9	4.56	32.4	73.3	55.1	10.6	40.1	106	166	235	715	573	2200	25.6	147	96.1	36.7	220	293	18.8	25.7	12.6	21.7	38	383	342	21400	12200	13.7	34.8	18.7	6.68	523	557	330	18.7	28.7	30.1	18.2	9.31	34.3	13.3	20.8	44.6	26.2	44.9	22.5	30.7	12.4	57.2	42.5	31	20.5	33.2	32.9
PGE1	0.641	0.912	0.605	1.06	0.61	2.85	1.57	1.28	4.61	1.18	0.655	1.47	0.634		0.644	0.785	0.847	1.01	0.274	2.73	1.72	1.04	1.01	1.31	0.55	1.23	0.121	0.19	0.242	1.77	2.41	0.272	0.232	0.0246		0.0545		0.0372	0.0716	0.225	0.0623		0.916	2.45	0.464	0.169	1.77	2.26	1.4	1.97	2.61	4.33	39.3	0.366	3.69	1.13	1.07	16.6	7.77	1.21	0.351	0.73	0.837	1.62	9.28	4.92	96.3	131	0.515	1.03	0.398		19.5	11.3	19.3	0.687	0.424	0.91	0.366	0.295	0.647	0.659	1.07	0.76	0.821	1.01	0.732	0.878	0.799	1.38	1.42	0.794	0.427	0.596	0.345
PGE2	20	4.89	14.1	16	6.33	23.7	29.8	23.4	59	8.73	9.98	43.3	4.19	5.38	5.35	3.66	15.2	21.8	7.88	38.1	45.1	7.61	4.91	4.73	40	142	14.2	19.2	16.5	69	85.3	12.4	7.76	2.65	12.9	6.93	19	35.3	9.14	4.13	3.54	2.49	42.3	63.8	48.6	32.8	174	98.3	106	151	133	181	706	14.5	104	38.6	27	282	152	61.6	62.5	32	71.6	164	536	151	3530	1590	19.6	51.7	27.8	4.88	534	356	360	20.2	22.1	24.4	7.97	8.14	7.4	11.5	7.71	12	7.58	16.8	11.7	22	11.3	35.7	19.5	13.7	8.5	12.8	5.4
15-Keto PGE2	6.66		0.192	0.124	0.202	0.39	1.18	0.923	1.47	1.32		1.13	0.938	0.868		0.0162	1.15		0.91	1.11	1.34		0.159		18.2	3.07		1.01	0.934		0.139	0.334		0.188	2.57	1.21	4.86	8.46	1.18	0.165	0.551	0.146			2.77	4.28	2.44	3.47	0.356	3.99	3.25	2.86	11.5	0.672	0.826	0.988	1.92	4.48	2.81	9.78	20.6	4.79	23	38.4	12.5	1.82	665	308	0.952	4.75	4.35	0.438	11.8	12.2	4.41	0.0306		0.208			0.287		0.21	0.107	0.674	0.087	0.0994	0.561			0.167	0.188	0.0962	0.178	
PGD2	18.8	1.55	3.06	10	1.45	9.14	4.38	15.6	15.8	6.03	0.459	18.4	2.35	4.07	0.823	0.684	6.19	14.5	3.08	11.9	14.2	4.76	0.232	2.3	76.8	13.9	55.7	27.7	48.1	6.82	31.8	16.1	3.62	2.73	34.7	30	50.9	115	25.1	6.99	8.68	5.45	54.2	57.3	197	15.2	30.1	11.6	19.1	24.8	98.8	120	235	15.2	105	29.2	38	48.8	70.1	15.9	50.3	8.2	29.2	67.2	85.8	65	6210	2850	12.7	45.5	15.9	14.2	226	144	54.9	17.9	18.1	21.1	5.24	6.57	14.1	7.79	8.41	8.33	8.66	20.7	16.3	21.1	12.8	25.7	18.6	8.22	3.41	7.28	3.17
PGF2a	16.1	2.84	9.8	9.38		5.12	3.72	15.2	15.7	15.8	1.9	14.7		0.561	4.47	2.01	14.6	4.41	6.77	27.5	18.7	3.83	6.08	6.35	5.9	4.95	2.46	5.08	2.67	30.7	10.8	4.37	2.74	0.529	5.02	3.81	4.1	7.29	4.75	0.376		3.38	6.54	7.35	5.96	1.71	8.02	4.26	10.2	11.1	44.4	38.5	170	12	15.9	7.58	5.04	28.8	68.8	14.4	5.18	4.48	10	7.33	52.8	25.2	2990	722	2.08	6.03	12.9	3.57	103	177	60.8	4.82	8.04	7.68	2.78	1.84	4.99	3.68	1.09	3.33	2.86	4.23	4.81	8.14	4.69	8.37	5.88	4.66	0.848	5.04	1.08
15-deoxy PGJ2	0.295	1.07	1.09	0.998	0.561	1.09	1.28	0.924	1.07		0.743	0.157	1.53	4.36	1.04	1.45	0.325	0.917	1.77		1.21	0.495	0.675	0.689	0.826	1.68	0.882	0.879	1.3	1.48	0.351	1.03	0.994	0.911	0.993	1.17	0.76	1.32	0.836	1.11	1.31	1.34	0.25	1.99	1.01	0.594	1.41	1.39	0.955	1.07	4.56	2.06	1.75	1.05	1.13	1.14	0.859	1.34	0.868	0.985	1.01	1.34	1.58	1.39	1.74	2.76	87.4	72	1.53	1.5	0.888	1.11	1.42	1.43	1.14	0.341	0.416	0.226	0.234	0.0599	0.0248	0.167	0.0849	0.203	0.252	0.07	0.0896	0.318		0.209	0.401	0.419	0.141	0.183	0.246
PGE3	1.75	0.367	1.52	3.9	1	1.99	6.5	5.07	5.19	1.51	1.59	1.08		0.144	0.00742	0.41	0.497	0.569		1.04	2.7	0.252		1.1	2.99	5.57	0.861	1.43	0.386	5.1	6	0.971	1.37	0.23	0.103	0.0988	0.265			0.151		0.452	1.08	1.86	2.35	1.84	10.7	4.19	6.19	7.44	8.21	16.8	42.2	0.186	7.73	3.07	1.83	24.1	11	0.645	0.541	0.421	0.535	0.822	2.57	24.8		51.2	0.881	1.88	0.606		15.7	5.39	11	1.28	1.37	2.77	1.33	0.966	1.05	0.789	0.838	1.28	1.56	0.65	0.477	1.02	0.443	0.842	0.504	0.833	0.618	0.399	0.195
PGF3a	0.704	1.15		0.295	0.558			2.79	2.8					1.09				1.45	1.89		0.183			0.889	2.62	0.524			0.298	2.15	1.25			0.257	0.238		0.529	0.885		0.36	0.474				0.386	0.965	0.436	0.455	1.8	0.808	2.63	4.54	21	0.332	1.08	0.738		0.188	6.47	0.552	0.978				1.7		22.8	97.8			2.41	0.779	1.89	4.95	2.65		0.933	0.567		0.417	0.295		1.1								0.554		1.75	0.222	
PGE2 EA	0.154	1.86	0.302		0.293	0.541	0.316		0.832	0.57		0.0962		1.05	0.685	0.233		1.15		0.435		0.396	0.38	0.278	0.42	0.405	0.235	0.333	0.41	0.458	0.3	0.4	0.484	0.492	0.233	0.388	0.373	0.216	0.182	0.446	0.291	0.312	0.261	0.0957	0.151	0.239	0.461	0.243	0.332	0.158	0.749	0.636	1.68	0.36	0.144	0.0957	0.154	0.44	0.423	0.137	0.442	0.106	0.129	0.216	0.202	0.866	1.81	1.49	0.321	0.227	0.31	0.361	0.349	0.303	0.363	0.116		0.213				0.101	0.119	0.175	0.0833	0.0671	0.0134			0.094	0.0769		0.112	0.093	
PGF2a EA	1.01	1.41				0.462			1.39			1.18					0.208			0.431				0.442	0.224		0.0676	0.13			0.191	0.278	0.172		0.0709	0.0198	0.205	0.249	0.104			0.0416								0.0281		0.239			0.19	0.0925	0.0349			0.0419							0.00283						0.0347					0.0202		0.262		0.0792			0.267					0.0623	0.058				0.179
PGD2 EA	3.96		2.33	1.57	1.95	1.07		1.82	1.71	1.48	1.7	1.46	1.65		2.64	2.92	1.05		1.63	1.18	2.35	1.52	1.34	1.36		0.239							0.18							0.165	0.232									0.181		0.349													0.117		1.48	1.64		0.179		0.179					0.707		0.206	0.176		0.0871	0.795	0.246	0.426	0.554		0.61			0.15			0.344	0.451
PGE2 1G	17.7	8.2	8.38	11.4	16.3	8.07			8.78	9.68	12.7	3.17	19.1	2.99	10.3	13.3	8.54	4.26	8.23	3.38	7.07	10.3	5.46	7.39	0.525	0.93	1.49	1.26	1.15	0.94	1.07	0.857		0.695	0.698	1.45	1.37	0.416	1.13	0.356	0.694	1.08	0.154	2.02	1.15	0.602	0.826	0.433	1.02	1.38	0.66	2.28	3.65	1.15	1.26	1.7	1.61	0.573	1.47	0.964	0.88	0.196	1.21	1.12	1.26	5.31	11.8	18.7	0.617	0.652	1.32	0.726	1.78	1.04	2.26	1.41	5.17	3.05		2.22	1.59	2.45		1.55	2.99	2.55	3.57	2.59	1.6	1.79	2.35	3.62		1.75	3
PGF2a 1G				1.7	1.46	1.03		0.162	0.184		1.45	1.9	1.84			0.934	0.951	0.88				1.88		1.63			0.279		0.878	0.638		0.4			0.247	0.473	0.434	0.253	0.277	0.435			0.106	0.185	0.286				0.067	0.132			1.67	0.347	1.16	0.642	0.0245	0.455	0.804	0.18	0.297		0.176	0.322			3	19.7	0.0512	0.0614	0.344	0.298	1.55	1.48	0.762		1.34	0.808		0.214	0.733	0.521		0.999	0.612	0.903		0.369	0.445	2.09			0.472	0.848	
9_12_13-TriHOME	14.3	2.75	2.92	28.6	7.07	19.9	60.5	25.5	32.4	9.17	9.24	16.1	13.3	1.69		3.87	6.4	3.66	11	67	17.1	14.2	55.1	29.1	2.53	5.42	3.31	0.734	1.84	20.8	7.69	3.18	1.4	3.1		0.356		2.23	0.718	0.838	0.0922	1.26	0.579	4.1	0.973	0.561		7	6.91	8.85	138	55.1	270	5.8	6.81	3.4	1.49	9.91	12.5	0.208	3.21	1.93	3.6	0.878	18.6	37.2	12000	13100	3.62	4.29	1.38	1.63	14.3	9.93	16.9	1.62	1.42	0.897	8.84	1.74	1.63	1.7	1.28	1.13	0.9	0.553	1.12	0.647	0.38	1.62	1.92	2.91	1.11	2.25	2.53
Lipoxin A4				4.54			2.44	5.2	4.17			0.619	4.21	1.89	2.91	0.961		3.56	4.33	6.59	0.515	4.14	3.12	2.26	2.26		2.67		0.619	2.57	2.57	1.28		2.43	3.8	1.33		2.03	1.13	3.49	3.59			1.05		0.802		1.33	1.53	8.16	5.6	2.11	10.5		4.27	0.481	1.38	0.547		0.574	2.23	0.57	0.114		3.14	12.9	4090	3140			1.39	1.87	0.521	2.06	0.667	0.367	0.751		1.24		0.927	2.79		0.44		0.413	1.92	2.88	0.467	1.3		2.96	0.746		0.436
LTB4	1.56		0.21	1.51	0.428	0.305	1.16		0.163	0.148			0.703	4.11		1.43		0.957	0.972	1.33		0.634	2.32	0.91	13.1		8.39	0.908	7	2.33	2.85	0.116	1.06		1.06	6.07	1.49	4.37	3.68	0.5	3.01		2.84	0.598	3.43	0.478	0.128	0.833	0.948	0.95	3.62	9.96	8.84		5.1	5.97	1.33	0.522	1.52	0.612	0.613	2.9	2.33	1.53	8.74	4.47			0.162	0.526	1.51	1.07	0.526	3.79	4.46	3.56	1.21	0.349	0.352	0.14	0.0832	6.22	1.78	3.2		0.707	7.44	9.33	5	5.87	1.41	0.39		8.71	8.63
LTB5	0.0868	0.0338			0.295	0.112	0.134							0.289	0.158	0.189	0.137	0.216	0.0546			0.261			1.54	0.0899	0.984	0.477	0.8	0.132	0.254	0.0877	0.0609	0.124	0.171	0.196	0.112	0.103	0.123	0.0394	0.0287	0.0547	0.209	0.297	0.433		0.0878	0.275	0.155	0.282	1.75	1.51	1.06	0.0987	0.354	0.386	0.131	0.34	0.399	0.097	0.0343	0.092	0.118	0.115	0.173	1.53			0.00518	0.0575	0.0306	0.126	0.325	0.123	0.124	0.266	0.282	0.406	0.328	0.0264	0.0705	1.01	0.304	0.807	0.149	0.146	0.239	0.274	0.39	0.0687	0.168	0.0416	0.599	0.407	0.558
6-trans-LTB4	0.459	0.155					0.995		0.275							0.278		0.869		0.248	0.76	0.972		0.114	0.165	0.27	2.1	1.14		0.0344		1.09			0.741	1.15	0.912	1.7				1.39	0.162		1.4	0.247	0.298	1.48	5	11.1	4.89	7.96	14.3		1.84	0.593		0.257	0.637				1.4	0.303	5.04	0.82	1240	868	0.33			0.306	3.42	0.814	2.41	0.0675			0.214	0.0342		1.36	0.0721	0.167	0.385	0.46	0.454	0.262		0.344	0.994			0.29	0.401
8_15-DiHETE	1.54	0.199	5.48	5.05		7.75					0.576	5.17	5.93	1.36	9.53	3.24	7.38	6.11	2.34				2.75	5.1	0.00794	1.83	3.26	0.574		15.6	0.148		1.17	0.351	0.112	1.56	1.26	0.345		0.17	2.37	0.384	1.39	1.12	0.0894	0.693	2.71	3.35	11.6	10.3	89	29	130	0.26	1.53	3.43	0.604	4.24	8.24		0.45	3.33		1.55	2.91	49.6	7940	4730	0.171	2.21	0.0361	1.48	8.58	6.48		0.702			2.28		0.00457		1.31	1.48		1.27	2.15	0.243		0.259	1.27	2.38	1.32	1.23	
5_15-DiHETE	1.63		1.23	1.99		0.0509			1.23					0.139	1.3		0.885				1.22		0.414	0.0263		0.0616	0.279		0.0499	2.78	1.05			0.0596	0.0728		1.06		0.0623		0.419	0.243	0.454	1.52	3.34				2.55	2.79	37.1	20.4	54.5	0.0573	7.79	0.995	0.235	0.566	3.51	0.0649	1.84	0.0388	0.247		6.28	28.9	3510	2140		0.792			2.43	0.856	1.38	0.418	0.436			0.0103	0.203	0.334		0.746		0.125	0.267	0.548	0.553	0.327	0.118		0.463	1.68	0.684
9_10-e-DiHO	47.3	16	22.6	55.3	26.5	58.3	134	58.7	25.5	16.8	23.4	41.8	85.3	8.02	19	19.9	10.2	24.6	22.1	31.2	36.6	14.1	62.4	72	49.3	8.55	74.9	11	8.52	27.2	24.3	10.9	3.65	7.97	3.56	5.31	2.96	8.15	2.01	6.68	6.05	9.54	5.99	27.6	23.3	23.3	11.8	262	241	290	69.6	111	144	12.4	66.9	51.4	69.3	868	429	9.52	41.2	25.8	36.7	32.4	593	89.7	793	1220	34.1	29.3	9.43	14.6	736	553	610	7.63	4.58	2.88	8.84	4.52	7.26	4.15	3.81	4.63	6.49	1.75	1.86	2.15	2.73	5.2	2.33	5.28	3.7	4.49	5.69
12_13-DiHOME				0.407		0.443	10.5	8.83	2.15	1.19		1								4.26	3.53	4.81	3.58	2.37	0.111	0.0718	0.189	0.00816		3.87	1.59	0.439	0.496	0.31		0.0288	0.0561	0.234		1.51	0.179	0.25	1.11	3.77		0.054	0.00217	3.57	2.17	3.37	3.66	3.01	6.38	1.57	1.08	0.281	0.58	7.65	5.49	0.0294	0.463	0.279	1.27		7.54	2.29	1460	2070	0.771	1.67	0.89	1.57	11.2	8.08	11.1	0.163			0.388	0.117	0.271	0.262	0.238		0.0151				0.341	0.106	0.129	0.345	0.0294		
9_10-DiHOME	1.82	1.05	0.266	3.39	0.422	5.28	30.7	31.1	3.74	3.46	1.19	3.13	10.7	0.79	0.176		0.658		0.565	6.98	6.19	5.04	10.8	19.2	0.122	0.0141	0.381	0.0496	0.102	3.51	1.74	0.146	0.149	0.389	0.207		0.0601	0.561	0.176	1.08	0.487	1.04	0.324	1.52	0.0158		0.00965	3.3	1.95	5.11	8.9	4.13	13.6	0.812	1.42	0.44	0.958	6.76	6.4	0.0941	0.335	0.496	1.12	0.195	7.06	2.77	3920	6590	0.834	1.7	0.733	0.994	13.2	8.48	10.6	0.493	0.305	0.259	0.45	0.292	0.315	0.704	0.232	0.406	0.135	0.236	0.259	0.0626	0.573	0.696	0.356	1.02	0.28	0.322	0.43
15_16-DiHODE	2.62		1.41	0.454	2.05		86.5	55.7	6.93	5.55		0.406			4.09	0.233	0.827		2.87	2.07	11.8	3.18	11.3	11.9	0.143		0.583	0.0886	0.275	4.84	2.35	2.41	1.38		0.37			0.119	0.89	2.33		1.03	0.498	4.31	1.46			0.753	0.31	0.252	1.78	6.65	1.74	5.82	0.318	0.319	8.14	2.08	10.9		0.313	0.635	0.325		0.177	2.19	98.5	459	0.141	0.0241	0.34	3.27	6.92	4.59	1.76	0.402		0.368	1.1	1.09	1.12	1.27	0.574	0.626	0.643	1.07	0.486	0.177	2.18	0.278	1.02	0.55	0.255	0.915	0.656
12_13-DiHODE		0.889	0.639	2.31	1.76	3.06	17.3	7.77	1.43		3.85	1.13	8.59							0.525	2.89	2.07	2.99	3.6	0.41		0.12	0.781		0.938	0.573	0.268		0.345	0.0789	0.0947	0.524		0.694	0.647	0.231	0.689		0.897		0.214	0.349	0.158			0.0198	1.51	2.71	0.855	0.257	0.18		0.762	0.839	0.463		0.147	0.387		0.573	0.841	35.7	237	0.418	0.769	1	0.0132	0.365		0.398	0.582	0.241			0.228	0.4	0.338		0.341		0.686	0.00512	0.867	0.334	0.506			0.313	0.259	0.42
9_10-DiHODE	0.346		0.255	0.092		0.935	22.1	11	1.29	2.58	1.17				0.0233			0.274		0.0637	1.02	1.65	3.87	1.95		0.16	0.207	0.0672	0.0257	0.546	0.351	0.141	0.625	0.0452	0.0221				0.0391	0.276	0.187	0.104	0.0991	0.246				0.122	0.0571	0.286	0.549	0.228	3.56	0.622		0.146		1.11	1.22	0.105		0.00489	0.105		0.0244	1.21	114	511	0.069	0.15	0.0714	0.323	0.547	0.384	0.236	0.0581		0.0602	0.295	0.0211	0.346	0.185		0.146	0.0998	0.0161	0.0384	0.0631	0.199	0.206		0.00801	0.0218	0.0592	0.0903
14_15-DiHETrE	1.54	0.935	1.67	2.13	0.207	0.828	0.766	0.457	1.84	0.00511	0.647	0.035					1.06	1.58	0.00903	1.33	2.01	0.00142	0.129	0.865	0.693	0.329	0.562	0.507	0.623	6.37	1.7	0.1	0.65		0.668	0.542	0.111	0.148	0.129	0.169		0.32	0.502	1.88	0.992	0.161	0.508	0.533	1.1	2.14	6.18	7.18	11.3	6.23	1.07	0.845	1.32	4.07	3.73	0.337	0.583	0.475	0.0553	0.197	1.33	4.25	3320	3320	2.46	3.26	1.83	7.99	5.63	8.08	3.7	0.298	0.429	0.104	0.328	0.0934	0.196	0.276	0.272	0.139		0.856	0.297	0.276	0.484	0.454	0.415	0.4	0.659	0.569	0.634
11_12-DiHETrE		1.06	0.264	1.77			0.945			0.244	0.465	1.35		0.468		0.576		0.0985	0.156	0.606		0.0697			0.222	0.0676	0.0993	0.534	0.025	3.72	1.89	0.129	0.382	0.0685		0.0126	0.148	0.0883	0.179	0.083	0.05		0.048	0.54	0.565		0.0435	0.192	1.42	1.61	3.23	3.56	7.25	2.1	0.143	0.307	0.772	1.12	1.79	0.151	0.347	0.283	0.138	0.116	0.986	3.64	4730	4470	0.351	2.11	0.689	5	2.47	3.76	0.582	0.194	0.0904	0.0168	0.211	0.0465	0.00527	0.277	0.139	0.324	0.253	0.41	0.261	0.29	0.382	0.476	0.343	0.401	0.54	0.292	0.824
8_9-DiHETrE					1.32	1.93	1.59					0.379					1.77	0.549		0.612			0.224			0.493			0.226	5.23	0.676		0.514			0.448			0.8	0.559		0.39		1	1.72		0.519	1.15	1.63	3.77	13.9	4.3	10.4	0.0643	0.0541			1.58		0.137					0.27	4.7	10700	9100			0.285	0.0385	0.313	1.87	0.639	0.425	0.196	0.392					0.416	0.461	0.631	1.07	0.53	0.371		0.382	0.687	0.674	0.72	0.573	0.219
5_6-DiHETrE		0.548		0.406		0.271	0.448	1.44	1.03	1.35		0.808	0.397	0.0442			0.273				1.73	1.12		0.87	0.0714	0.00609	0.0321			5.58	0.714	0.0202			0.0198	0.034	0.0109	0.0308				0.00757	0.124	0.881	0.149	0.0225	0.022		0.944	0.436	6.48	3.59	12.2	0.0267	0.388	0.266	0.0276	0.224	0.205	0.0316		0.63		0.0293	0.0233	0.789	13400	10300	0.151	0.00834	0.269		0.132	0.272	0.278	0.0394			0.27	0.11	0.247		0.0405	0.224	0.211	0.0647	0.00217		0.146			1.19	0.184	0.408	
17_18-DiHETE	29.1	5.52	29.9	54.3	26.2	37.6	42	16	18	11	17.4	11.1	16.7	15.4	19.7	14.9	4.31	28.8	3.23		11.7	8.25	2.09	2.75	0.343	2.18		0.674	5.17	16	10.3	5.48	6.6	3.62	0.153	4.22		0.00682	0.915	3.34	1.5	5.91	0.814	7.05	3.24		2.44	5.85	2.98	6.84	38.4	29.2	53.7	64.6	4.52	1.93	81.1	47	73.9	3.98	2.48	0.789	5.45			24.8	1940	9210	12.8	25.8	11.4	48.9	58	17.9	5.13	7.22		0.666	2.87	1.89	8.65	2.03	1.41	2.81	4.72	1.4	2.34	0.294	2.76	1.46	0.952	1.86	2.97	1.63	0.65
14_15-DiHETE	12.4	5.4	1.13	1.39	13.9	8.93	3.23	0.627	13.9	2.24		21	0.00859	0.983		8.02	4.83	7.69	8.73	8.43	3.98		9.36	12.4	3.12	0.269		0.046	1.12	8.49	4.04		1.59	2.88		0.566			3.13	4.44	7.87		2.34	0.125	7.66		0.549	2.68	8.86	1.38	3.92	15.7	25.3	12.8		5.56	4.88	11.1	17.1	4.95						13.6	1810	7720	7	4.19	1.53	6.59	0.249	6.43	0.205	3.95		3.39	1.94		3.06	0.479	2.19	1.3			5.94		4.54	0.279			0.288	0.225	
19_20-DiHDoPE	2.76		4.06	3.74	2.11	3.87	2.3	1.79	5.4	2.57	2.66	0.532	0.986	1.7	0.616	3.9	0.989	1.2	1.53	1.52	1.56	0.583	3.12	2.05			0.706	1.16	0.918	1.53	0.862	0.54	0.228			1.3	0.239			0.385	0.0973	0.493	0.513	0.78	1.5			1.11	0.965	0.299	5.9	2.57	8.95	3.23	0.193		2.2	2.94	2.2	0.436	0.13	0.195	0.193		0.481	1.41	367	728	1.21	1.12	0.466	2.47	2.28	1.68	1.42	0.545	0.126	0.371	0.923	0.555	0.256	0.771	0.421	0.844	0.456	0.387	0.382		0.342	0.365	0.387	0.333	0.159	0.755	0.359
13-HODE	28.9	10.4	25.4	53.4	12	131	223	158	392	39	39.6	31.2	19.5	7.41	10.6	6.59	95.7	30.3	39.5	151	89.8	167	233	174	9.1	4.25	4.81	19.2	9.75	149	35.8	4.22	7.59	1.58	1.19	1.97	4.1	4.46	2.7	4.35	1.02	4.53	17.5	45.6	3.21	2.43	3.44	27.6	25.8	42.7	1100	561	2540	11	104	55.4	14.8	106	204	2.79	6.64	4.04	12	6.59	155	428	41400	41000	17.2	17.8	11.6	4.93	380	250	328	21.3	12.9	14.2	9.28	3.57	9.69	26	22.1	32.9	15.8	31.3	14.1	16.5	12.1	36.2	64	76.8	25	38	24.8
9-HODE	23.1	4.4	11.4	27.6	6.57	77.2	115	150	110	31.9	22.5	47.4	11.9	11	9.28	4.1	64.5	10.8	20.9	107	40.3	53.3	82.3	61.8	52.2	16.6	25.1	44.7	34.6	287	105	24.8	9.68	5.28	7.77	5.16	17.8	19.8	9.52	10.2	6.52	4.51	56.4	156	7	5.49	10.5	39.9	38.3	52.7	1260	763	3100	13.2	208	154	17.8	268	366	6.16	16.6	5.55	18.1	15	212	499	31300	30000	40.2	36.7	20.4	10.1	640	535	581	32.7	43.2	50.9	25.7	9.77	33.2	51.2	68.5	92.3	60.3	114	43.9	59.6	34.4	121	234	152	52	92.7	45.8
13-HOTE	1.74	5	3.72	5.19	0.61	11.5	161	43.1	205	5.58	6.23	4.91	1.1	2.54	2.19	1.77	6.68	1.76	5.64	8.05	20.4	38.1	56.8	22.4	1.34	1.42	0.525	0.836	1.02	12.6	1.97	0.497	3.09	1.01	0.516	0.782	0.619	0.474	0.699	1.31	0.695	0.746	1.02	2.35	0.571	0.399	1.11	1.11	0.678	1.03	78.1	27.7	110	6.79	11.5	2.99	1.74	9.92	19.8		1.5	0.206	0.959	0.755	1.16	21.4	777	2440	0.505	2.82	0.397	1.92	10.6	5.22	5.54	2.37	0.741	0.397	1.02	0.371	1.31	1.07	1.16	0.811	0.61	0.435	1.12	0.305	1.01	0.407	0.586	1.33	0.578	0.617	1.16
9-HOTE	0.59	0.662	0.78	1.17		2.32	12.5	14	7.44	2.33		1.53	0.925		1.33	0.212	1.79	0.462	0.924	2.98	0.923	3.12	15.3	3.57	0.936	0.51	0.216	0.547	0.359	6.88	1.06	0.485	0.288			0.273	0.0445		0.00206	0.619		0.326	0.103	0.947	0.24		0.122	0.182	0.356	0.223	30.6	17.9	58.9	1.19	1.51	1.47	0.19	3.07	2.84	0.0963	0.328	0.0325		0.204	0.239	11.3	622	2170	0.0477	0.166	0.238	0.0534	1.83	0.57	0.677	0.966	0.126	0.122	0.263	0.0509	0.172	0.897	0.255	0.91	0.395	0.308		0.0428	0.0452	0.514	0.573	1.81	0.157	0.788	0.37
15-HETE	70.2	19.6	45.9	81.8	36.3	70.5	23.4	16.8	54.3	0.842	4.41	43.1	43.2	50.5	34.9	38.6	73.4	83.2	3.6	18.2	44.3	23.4	16.2	19.1	9.43	19.4	14.8	8.74	8.06	401	53.7	9.97	4.67	2.31	15.6	13.9	16	19.9	11.4	1.42	5.83	3.24	21.3	40.2	72.2	9.62	33.2	97.2	197	281	667	513	1680	18.5	127	63.2	22.9	126	238	12.7	19.6	6.57	18.2	14.4	302	380	40100	26700	9.64	15.4	7.36	6.38	297	358	214	9.66	19.5	17.9	10.6	5.76	13.3	10.6	13	26.4	14	27.8	15	23.4	10	37	31.4	55.1	28.3	48.1	40.9
12-HETE	411	355	284	457	67.5	1080	652	134	1510	70.5	104	517	481	136	230	210	324	969	123	440	459	337	566	334	6.85	103	32.2	32.6	19.2	400	163	11.5	25.6	12	36.9	159	34.5	97.3	4.7	22.4	80.5	22.2	18.5	273	215	73.5	103	448	1220	996	1210	612	1160	101	1100	304	300	433	1770	40.8	279	35.9	129	28.6	1420	325	21500	14600	28	84.4	60.1	52.5	1410	2150	1460	24.8	174	11.5	4.1	15.3	27.6	60.5	29.6	23.8	3.83	28.3	54.1	21	33.7	49.1	26.3	24	35.3	19.2	27
9-HETE		5.65	3.74	5.66		6.83	1.41	6		2.29	2.29	2.12	4.36	3.83	1.54	4.09	6.38	9.41	6.83	8.22	1.68		3.51		0.571	0.548	2.29	0.409	1.31	55	11.1	0.833	0.848	1.19	2.19	3.32	1.04	2.29	1.87	1.07	1.16	0.368	0.913	3.15	5.77	0.463	3.42	6.2	16	20.2	190	108	242	3.51	3.2	2.19		12.8	4	3.97	1.67	0.288	2.42		11.3	108	17600	9690	0.888	2.3	0.323	0.447	8.47	8.89	2.92	1.7		0.902	0.934		0.833	0.764	1.19	2.02	2.17	3.6	0.434		0.863	3.16	1.02	5.98	4.12	4.39	4.03
8-HETE	11.6	10.7	3.93	2.75	1.69	5.49		2.7	3.29	5.1	2.7	5.89	0.299	4.08	0.203	2.82	0.584	0.146	1.59	5.06	8.57	4.09	2.17	0.418	0.412	1.82	1.11	0.365	0.000717	29.1	5.25	0.701	0.164		0.502	1.4	0.702	0.519			0.57	0.268	1.21	1.78	3.06	0.436	0.914	3.54	8.69	14.5	109	52.3	111	1.02	2.85	1.08	1.87	1.3	6.85	5.37	1.88	0.246	1.45	2.43	8.34	51.3	10500	7060	0.107	0.27	1.19		4.98	6.69	6.93	0.204	0.421		0.18	0.294	0.0814	0.127	1.17	0.388	0.162	1.14	0.865	0.702	0.896	1.37	0.284	2.24	2.96	1.91	2.72
5-HETE	111	7.25	15.3	58.4	2.51	41.2	5.86	21.7	61.8	11.7	0.767	165	5.82	0.786	12	11.7	60	29	6.56	20	46.3	4.86	3.46	7.41	59.1	35	106	57.4	33.7	246	84.1	6.58	6.3	3.03	134	142	200	131	73.8	14.1	31	15.2	114	97.3	333	37.9	19.5	34.8	115	210	288	242	386	6.7	97.9	48.9	42.1	25.4	17.7	26.7	65	18.9	51	48	139	273	37900	28100	16.9	44.1	24.6	7.67	58.5	43.2	48.3	42.6	64.8	74.3	11.1	10.9	14.1	65.8	38.9	57.1	10.6	78.9	145	156	74.3	58	38.6	62.1	84.2	209	263
15-HETE EA	0.283	0.126	0.119	0.382	0.23	0.283		0.119	0.0486	0.338		0.424	0.151			0.135		0.0832			0.0918	0.212	0.206	0.157		0.0094	0.0172		0.0028	0.0277						0.00472	0.0116	0.0366	0.022				0.00285			0.0247	0.0153			0.0413	0.115	0.0709	0.343	0.00173		0.0057				0.0105	0.0268				0.0193		37.4	42.6				0.00583		0.0278	0.0215		0.0402	0.0446			0.0112			0.0538	0.0632		0.031	0.0614				0.0823	0.0288		0.0751
15-HEPE	29.2	11.8	36.2	60.2	12.4	58.4	82.7	24.6	162	11.9	8.47	25	5.55	8.14	7.85	2.99	6.25	19.4	4.08	9.47	12.6	11.9	6.03	5.5	1.93	4.23	3.35	3.42	3.69	59.6	12	0.503	3.48	1.47	0.841	0.723	0.448	0.882	0.294	0.123	1.83	1.05	0.745	5.79	5.04	1.35	3.01	14.6	26.3	34.8	136	88.8	213	6.81	54.7	19.8	13.7	31.3	70.8	0.742	0.849	0.209	0.656	1.04	2.27	87.5	1750	3990	0.405	2.89	2.82	2.15	25.3	26.3	15.3	2.41	2.12	4.55	2.62	1.35	6.17	4.86	4.8	10.4	2.57	1.36	1.85	1.25	0.922	1.58	1.36	2.74	2.4	4.11	2.74
12-HEPE	56.4	50.4	46.6	121	12.1	205	310	49.9	394	25.1	30.7	43.4	18.5	18.1	12.3	5.63	19.3	36.9	6.51	19.8	32.5	25.9	40.1	30.3	1.29	10.2	3.81	1.26	2.54	100	26	2.18	4.93	1.36	0.764	11.2	0.395	0.893	0.00758	1.79	2.41	1.7	1.28	13.1	18.4	3.58	9.01	82.5	212	193	675	217	451	17.7	140	47.9	40.4	96	295	0.815	2.54	0.848	2.46	0.202	19.2	154	1370	3650	2.99	5.6	3.71	6.05	90	98.5	83.1	7.4	32.6	2.62	1.7	2.86	6.36	23.1	7.69	4.51	1.35	1.94	4.19	1.32	3.28	1.45	0.924	1.75	4.74	2.85	2.34
9-HEPE	5.42	0.557	1.01	2.61		0.556	2.08		0.318					13.8	1.24		0.14		0.695		3.61		0.0359			0.958		0.5	0.415	14.1	1.61	0.194	0.0874	0.233			0.218	0.506	0.416	0.498		0.106		1.02	0.502	0.819		0.452	4.34	2.36	60.5	25.8	60.6		0.49		0.714					0.827				44.1	1120	2880	0.577	0.328					0.444	0.554	0.41				0.357	0.394		0.709	0.111	0.00722			0.0392	0.118		0.381		0.538	0.927
5-HEPE	56.6	1.33	11.2	29.3	3.07	14.7	3.74	17.6	30	13	4.37	46	1.99	7.44	1.32	0.474	6.39	2.29	1.97	3.07	12.9	0.357	1.89	2.94	23.1	11	35.2	22.3	11.1	90.8	22.7	3.63	3.76	1.54	6.25	6.58	5.19	2.92	1.84	3.57	5.52	1.68	13.7	25	70.4	11.5	7.21	6.91	20.2	47.8	121	83	115	1.41	27.4	14.4	16.8	10.8	3.89	2.24	1.54	0.522	1.76	2.32	1.46	147	2210	6190	1.97	7.57	3.22	3.16	6.7	4.74	5.75	8.6	18.3	30.9	1.94	3.01	7.8	37.3	23.2	26.7	5.24	4.57	13.6	15.5	7.74	3.73	5.72	7.97	22.6	49.7	50.8
17-HDoHE	111	90.4	75.1	112	41.3	131	227	40.8	351	8.75	27.9	51	65.5	26.1	43.5	39.9	22.6	78.8	11.4	12.4	104	7.58	61	37.9			5.48	2.31	2.11	61.3	4.87	0.522	5.29		5.22	0.863			4.05			3.5		2.99	28.9	4.15	26.6	62.6	107	45.1	222	63.1	263	4.54	28.9	23.1	14.1	31.1	84.5		5.4	2.67	3.77		62	73	5930	7170		4.45		2.29	68.3	111	31.3	2.82		1.11	1.65			1.84		6.2	1.77		2.05			1.86	3.18	1.49	4.32	5.64	
14-HDoHE	241	232	201	273	89.3	475	530	106	1050	72.4	67.5	257	292	76.9	94.1	91.6	199	446	72.5	123	219	158	248	134	9.26	40	17.5	13.9	8.25	132	15.2	1.9	15	1.68	3.59	13.5	4.03	10.8		3.65	8.19	3.87	2.1	8.11	47.6	27.5	11.7	189	309	297	317	110	246	20.7	170	62.2	47.7	139	263	2.72	30.2	6.4	17.3	5.38	196	74.4	2110	2350	4.4	15.9	13.9	14.4	197	252	126	9.72	2.63	4.55		0.76	4.87	5.19	4.96		2.67		3.42	2.66	3.8			2.21	5.54		3.55
4-HDoHE	11.6	3.1		2.85	1.97	2.77	6.62	4.28				4.06	5.97	1.79	7.47	7.71	5.87	8.3		2.51	6.32	2.26		2.36	0.406	9.06	1.38	2.19		17.7	2.11	1.35	0.49		0.774			1.58	0.819	0.692			0.376		25.3			6.78	21	53.3	49.1	6.35	56.3		1.48	0.932				0.392	1.29		0.362	0.839	8.53	10.3	3630	4220	1.63	0.97				0.868	0.898	0.513		0.901	0.945	0.615	0.224	1.18	0.269	0.64	0.84	0.677	0.324		0.567		1.03	1.68	2.73	3.2	3.69
9_10-EpO	244	68.4	259	244	136	248	269	103	266	226	99.2	136	122	19.2	140	227	174	222	188	198	90.6	155	143	181	21.9	18.7	28.7	10.3	4.2	18.9	15	13.6	2.55	19	7.21	17.4	5.85	26.3	7.65	11.6	3.25	10.8	11.1	5.35	12.1	13.5	2.22	116	15.5	137	29.1	49.5	33.6	17.3	99.5	19.8	34.3	60.6	35.8	10	37.9	28.9	18.9	8.62	219	61.1	1720	3420	18	13.6	10.7	16.4	176	54.8	176	49.9	33.3	37.7	56.2	20.5	36.5	64.9	36.2	45.9	26.5	32.2	26.8	22.8	25.4	43.6	56.2	36.2	29	24.3	41
12(13)-EpOME	4.41	1.02		0.771		8.82	3.14	10.2	4.1	3.11	0.251				1.33		12.2			17.5	2.99	2.47	9.77	10.8				0.541		1.3	2.51	1.31	1.79	2.03	0.0649	0.309	0.118		0.413	0.0395	0.153	0.791	0.572	0.777				0.85		0.94	2.4	0.431	1.48	0.619	0.389	0.638	0.356	0.6	1.95		0.118	2.22			1.54		511	1570	0.307	0.695	0.359	0.00046	2.44	1.25	3.66	2.97	0.167		1.33		0.107	4.96	0.487	2.88	2.17	0.75	0.189	0.796	0.158	0.377	0.553	2.18	1.41	0.691	1.24
9(10)-EpOME	0.35	0.499		1.47	0.646	7.64	5.21	10.7	5.93	0.959	1.03	3.32	4.3	0.0774	0.092		6.49		1.76	7.72	3.74	1.11	7.29	2.78	0.162	0.02	0.275	1.23	0.451	2.33	0.671		0.267	1.08	0.525		0.0666		0.064				0.934	0.599	0.0418	0.198		0.853	0.0816	0.54	0.913	2.39	3.75	1.11	1.24		0.502	0.512	1.37	0.196	0.262	0.991	0.57	0.115	1.63	1.13	1490	3000	1.22	0.416	1.11	0.539	2.78	2.03	3.42	2.52	0.301	0.183	0.834	0.0619		3.49	1.12	1.41	0.54	0.207	0.189	0.488	0.123	0.839	1.26	1.18	0.822	0.712	1.23
15(16)-EpODE	3.16	1.77	4.12	3.27	0.87	5.87	6.46		4.74		2.83		2.75	1.54			1.17	3.51	0.53	6.8	3.45	3.66	4.3	7.57	0.526		1.55		0.235	0.739	1.06		0.725				0.524					0.622		0.151		0.777			0.114	0.531	3.22	0.546	4.41	1.11			0.956	0.992	3.67	0.837	0.0933			0.288	0.546	2.1		144		0.778		1.78	1.85	1.87	1.25	0.916	0.0588	0.649	0.392		0.761	1.34	0.433					0.394	0.501	0.362		1.75	0.791	0.796	
12(13)-EpODE	0.269	1.28		0.374					0.489	4.01		0.192	0.0304		1.1		1.08	0.0674					1.66									0.0242	0.0354	0.534	0.0572				0.194	0.0345	0.0383	0.0486	0.401	0.31		0.159			0.0318	0.0499		0.248				0.145	0.207	0.366	0.0636					0.349			3.23	33.9		0.477		0.182		0.0422		0.216		0.682					0.101	0.368		0.0253		0.3	0.281	0.00419	0.00768				
9(10)-EpODE	0.979		2.19			3.86	7.34	5.83	7.38	4.91	3.48		0.989	0.74	0.122		2.84		1.2	2.61	1.91	2.13	3.69	2.81	0.673		0.804	0.317		1.19		0.784	2.11	0.342	0.345	0.463	0.374	0.162	0.097	0.284	0.0588	0.607	0.667	0.646	0.366	0.348	0.0164	0.556	0.19	0.47	3.14	1.46	4.34	0.648		0.705	0.162	0.71	1.4			0.729	0.391	0.678			182	291	0.156	0.0773	0.221		0.923		1.41	0.201		0.232	0.404	0.225		1.7	0.337	0.925	0.44	0.0689	0.43	0.222	0.16	0.182	0.57	0.901			
14(15)-EpETrE		1.55	0.36	1.15	0.412	1.16	0.374		1.52	1.46	0.173	2.06	2.04			1.65	0.608	0.847		0.294	0.307		1.89	0.647	0.23	0.22	0.947	0.655	0.486	1.4	1.16		0.586		0.203	0.669	0.324	0.196				0.913	0.143	0.382	0.0136	0.535	0.784	0.108	0.977	1.11	0.153	0.105		0.432	0.722	0.213	0.00364		0.917	0.158	0.297	0.5	0.488		0.931	2.37	482	406	0.357	0.172	0.0121	0.578	1.32	0.776	1.72	0.144	0.38	0.195	0.208	0.179	0.18	0.733	0.79	0.937	0.308	1.16	0.326	0.222		0.444	0.778	1.85	0.0929	0.58	1.19
11(12)-EpETrE		0.608					0.322	0.71				0.572	0.0544			0.469	1.2	0.763		0.243	0.23	0.832	0.153	0.553	0.858	0.412	0.21	0.0444	0.155	0.446		0.167	0.134	0.0222		0.388				0.199			0.163		0.255	0.115		0.474	0.64	0.434	1.37	0.7	2.07	0.518			0.185	0.0603	0.497	0.19	0.111	1.15					235	328	0.193	0.23			0.812	0.677		0.138			0.00654	0.0541	0.198	0.239	0.0425	0.422	0.156	0.122	0.131	0.0854	0.0586	0.616	0.243	0.494	0.24	0.147	0.268
8(9)-EpETrE			1.75						5.04					0.59		3.28	0.339									2.26	0.599	0.649		5.1	2.06	1.37	1.92	0.471	0.442	1.13		1.13						2.23	0.376	0.101		1.57	0.861	1.66	3.56	0.199	95.2		1.23			0.3	0.544		0.796	1.96	0.0969		0.729		423	444	0.884	0.825	0.443	0.827		0.382	1.24	1.3	0.508			0.155	0.908	2.1			1.87		0.762	0.141	0.0512	1.16	0.792	3.84			1.69
20-HETE				52.5	78.5									75.7			50.8						37		10.8					16.1					12.4	12.6						16		14.2		10.2	11.3			9.33		26.5		14.4	9.88				11.3		9.14			17.4	14.2	28.9			10.8				15.7								21			10.7		15.3		10.7							9.49
11(12)-EpETE			0.0757			1.13			0.905	0.779	0.755	1.82			0.379						0.369			0.938	0.981	0.904	0.726	0.174			0.86	0.517	2.21	0.652				0.256		0.512	0.416	0.673	1.21		1.2		0.335	0.624		0.52	1.5	1.92			0.613	0.26		0.411			0.174	0.166	0.499					29.2			0.223	0.166	0.231	0.386		0.0759	0.00343		0.415			1.69	0.726			0.131	0.035		0.322		0.0678	0.189		0.524	1.41
19(20)-EpDPE	3.44	1.03	2.16	1.73	3.98	3.01	6.82	3.78		1.24	2.95	1.66	3.98	0.665	2.68		5.31	0.714	1.3	0.675	1.05		0.543	0.409	2.14	3.67	5.17	0.395	1.18	28.3	3.01	1.42	2.27	0.283	0.671	0.884	1.25	1.18	0.408			0.226	0.453	0.918	13.4	0.693	1.16	6.94	12.8	22.1	18.1	10.7	22.4	0.499	0.686	0.76	0.85	1.36	0.753	0.398	0.202		0.58	0.991	7.94	8.81	1830	1840	0.535				0.42	0.385	0.303	0.399	0.718	0.731	0.33	0.353	0.735	0.582	0.68	1.6		0.647	0.385	0.579	0.0922	0.591	0.608	4.01	2.38	4.1	4
12(13)-Ep-9-KODE	10	1.04	7.46	25.7	21.9	43.5	10.4	15.3	15.3	13.4	13.2	12.7	5.36		8.32	8.43	37.7	5.94	14.3	7.71	16.3	11	11.6	13.8						0.0944	2.62	0.556														0.823		0.0951	0.287	0.777	7.56	9.96	16.1	0.778	0.422		0.698	1.07	4.29		0.248			0.00935	1.8	4.16	238	639		0.596	0.456		2.55	2.82	3.8	8.06	2.03	1.22	13.6	0.592	0.871	6.24		4.54	2.88	0.804	0.771	1.88	0.716	0.325	0.968	2.79	1.8	1.2	4.52
13-KODE	0.707				2.36	2.19	261	102	165	31.3						0.645	8.08		3.51	125	51.2	12.1		12.8	2.66		1.43	2.44	0.979	5.65	1.46			3.92	2.13			2.48		0.814	0.669	0.113	4.84			0.487	3.06	23.4	3.27	8.97	122	31.6	290		7.6	4.09	4.94	14.3	19.1	2.42	4.03	5.98	10.1	5	28.4	2.69	10100	12100	0.377	6.92	3.17		25.9	11.9	22.5	3.54	0.591	0.423	0.63	1.28		0.62		0.62		0.666							2.42		2.67
9-KODE	3.7	5.22		17	2.48	67.2	244	99.7	94.6	33.8		29.1			9.15		68.7	3.19	11.1	198	38.4	11.3	40.2	21.8	0.0458	1.31		1.46		18.5	0.845	2.56		2.12										0.293		7.91	0.47	25.8	9.18	19.6	210	74.7	659	0.435	18.4	9.9	5.29	17.7	44.7		8.04	3	8.78	1.83	44.6	11	23100	26400	5.42	3.1	6.93	1.69	48.7	49.8	72.3	11.2	1.33	1.67	5.77	0.368	1.05	10.5		3.89	2.19	0.965		0.382	1.01	2.76	0.88	2.19	1.34	2.35	8.21
15-KETE	9.41		0.95	4.16			21.8	11.2	31		1.1	2.52	2.58	4.43	2.47		4.44	3.41	2.82	13.1	16.4		1.46	1.45	1.02	0.374	0.159	0.361	0.89	8.66		0.465	0.683			0.712	0.791		0.364	1.6	0.723	0.75	1.13	0.732	2.09		0.817	4.11	2.78	10.2	32.6	22.6	63.7		1.27		0.612	2.99	3.1	2.38	2.38	1.76	0.562	2.96	9.86	15	7550	7840	1.1	0.631		0.556	7.61	3.52	4.55	0.388	0.414	0.531	0.453		0.102	0.716	1.63			0.289			0.0892	0.873	0.555		0.856		0.968
5-KETE	1.95	1.19	0.872		2.15	0.5	0.446		1.7		1.11	3.7	0.892	1.19		1.7	0.166	1.21	0.591	1.2	1.58	2.05	0.816		0.561	2.28	0.428	1.51	0.755	15.9	2.73	0.427	1.4		1.74	1.01	1.24	0.66	0.517	0.553	0.717	0.65	2.43	1.42	6.18	1.09	0.208	8.12	6.94	15.9	33.2	21.5	53.2	0.255	2.63	0.115		0.147	0.962	0.226	1.51	1.51	0.145	0.564	10.5	5.28	8030	6040		1.22	0.691		1.32	2.93	1.49	0.031	0.791	0.952	0.0364	0.389	0.273	0.223	0.0638	0.978	1.38	1.87	1.72	1.03	0.236	0.794	0.9	2.02	2.43	3.48	7.62
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	PubChem CID	KEGG	Parent Lipid	Class	HMDB ID	InChIKey
6-keto PGF1a	5280888	C05961	C20:4n6	PG	HMDB02886	KFGOFTHODYBSGM-ZUNNJUQCSA-N
TXB2	5283137		C20:4n6	TX	HMDB03252	XNRNNGPBEPRNAR-JQBLCGNGSA-N
11-HETE	5312981		C20:4n6	R-OH	HMDB04682	GCZRCCHPLVMMJE-RSPKXIRXSA-N
PGE1	5280723	C04741	C20:3n6	PG	HMDB01442	GMVPRGQOIOIIMI-DWKJAMRDSA-N
PGE2	5280360	C00584	C20:4n6	PG	HMDB01220	XEYBRNLFEZDVAW-ARSRFYASSA-N
15-Keto PGE2	5280719		C20:4n6	PG	HMDB03175	YRTJDWROBKPZNV-KMXMBPPJSA-N
PGD2	448457	C00696	C20:4n6	PG	HMDB01403	BHMBVRSPMRCCGG-OUTUXVNYSA-N
PGF2a	5283078		C20:4n6	PG	HMDB01139	PXGPLTODNUVGFL-UAAPODJFSA-N
15-deoxy PGJ2	5311211	C14717	C20:4n6	PG	HMDB05079	VHRUMKCAEVRUBK-GODQJPCRSA-N
PGE3	5280937	C06439	C20:5n3	PG	HMDB02664	CBOMORHDRONZRN-QLOYDKTKSA-N
PGF3a	5280940		C20:5n3	PG		SAKGBZWJAIABSY-SAMSIYEGSA-N
PGE2 EA	5283119		C20:4n6	Ethanolamide		GKKWUSPPIQURFM-IGDGGSTLSA-N
PGF2a EA	53481911		PGF2a	Ethanolamide	HMDB13628	XCVCLIRZZCGEMU-FPLRWIMGSA-N
PGD2 EA	5283120		PGD2	Ethanolamide	HMDB13629	KEYDJKSQFDUAGF-YIRKRNQHSA-N
PGE2 1G	52193688		C20:4n6	Glyceryl ester	HMDB13043	RJXVYMMSQBYEHN-SDTVLRMPSA-N
PGF2a 1G	24778485		C20:4n6	Glyceryl ester		NWKPOVHSHWJQNI-OMVDPNNKSA-N
9_12_13-TriHOME	9858729	C14833	C18:2n6	Triol	HMDB04708	MDIUMSLCYIJBQC-MVFSOIOZSA-N
Lipoxin A4	5280914	C06314	C20:4n6	Diol	HMDB04385	IXAQOQZEOGMIQS-SSQFXEBMSA-N
LTB4	5280888	C05961	C20:4n6	Diol	HMDB02886	KFGOFTHODYBSGM-ZUNNJUQCSA-N
LTB5	5283125		C20:5n3	LT	HMDB05073	BISQPGCQOHLHQK-HDNPQISLSA-N
6-trans-LTB4	5283128		C20:4n6	Diol	HMDB05087	VNYSSYRCGWBHLG-UKNWISKWSA-N
8_15-DiHETE	53480358		C20:4n6	Diol	HMDB10219	NNPWRKSGORGTIM-RCDCWWQHSA-N
5_15-DiHETE	5283158		C20:4n6	Diol	HMDB10216	UXGXCGPWGSUMNI-BVHTXILBSA-N
9_10-e-DiHO	441460		C18:1n9	Diol		VACHUYIREGFMSP-SJORKVTESA-N
12_13-DiHOME	10236635	C14829	C18:2n6	Diol	HMDB04705	CQSLTKIXAJTQGA-FLIBITNWSA-N
9_10-DiHOME	9966640	C14828	C18:2n6	Diol	HMDB04704	XEBKSQSGNGRGDW-YFHOEESVSA-N
15_16-DiHODE	16061068		C18:3n3	Diol	HMDB10208	LKLLJYJTYPVCID-OHPMOLHNSA-N
12_13-DiHODE	16061067		C18:3n3	Diol	HMDB10201	RGRKFKRAFZJQMS-OOHFSOINSA-N
9_10-DiHODE	16061066		C18:3n3	Diol	HMDB10221	QRHSEDZBZMZPOA-ZJSQCTGTSA-N
14_15-DiHETrE	5283147	C14775	C20:4n6	Diol	HMDB02265	SYAWGTIVOGUZMM-ILYOTBPNSA-N
11_12-DiHETrE	5283146	C14774	C20:4n6	Diol	HMDB02314	LRPPQRCHCPFBPE-KROJNAHFSA-N
8_9-DiHETrE	5283144	C14773	C20:4n6	Diol	HMDB02311	DCJBINATHQHPKO-TYAUOURKSA-N
5_6-DiHETrE	5283142	C14772	C20:4n6	Diol	HMDB02343	GFNYAPAJUNPMGH-QNEBEIHSSA-N
17_18-DiHETE	16061120		C20:5n3	Diol	HMDB10211	XYDVGNAQQFWZEF-JPURVOHMSA-N
14_15-DiHETE	16061119		C20:5n3	Diol	HMDB10204	BLWCDFIELVFRJY-QXBXTPPVSA-N
19_20-DiHDoPE	16061148		C22:6n3	Diol	HMDB10214	FFXKPSNQCPNORO-MBYQGORISA-N
13-HODE	6443013	C14762	C18:2n6	R-OH	HMDB04667	HNICUWMFWZBIFP-IRQZEAMPSA-N
9-HODE	5282945		C18:2n6	R-OH	HMDB10223	NPDSHTNEKLQQIJ-SIGMCMEVSA-N
13-HOTE	10469728		C18:3n3	R-OH	HMDB10203	KLLGGGQNRTVBSU-JDTPQGGVSA-N
9-HOTE	53480359		C18:3n3	R-OH	HMDB10224	YUPHIKSLGBATJK-OBKPXJAFSA-N
15-HETE	5280724	C04742	C20:4n6	R-OH	HMDB03876	JSFATNQSLKRBCI-VAEKSGALSA-N
12-HETE	5312983		C20:4n6	R-OH	HMDB06111	ZNHVWPKMFKADKW-FYMOKONMSA-N
9-HETE	5312978		C20:4n6	R-OH	HMDB10222	KATOYYZUTNAWSA-DLJQHUEDSA-N
8-HETE	5283154	C14776	C20:4n6	R-OH	HMDB04679	NLUNAYAEIJYXRB-VYOQERLCSA-N
5-HETE	5280733	C04805	C20:4n6	R-OH	HMDB11134	KGIJOOYOSFUGPC-JGKLHWIESA-N
15-HETE EA	91886095		C20:4n6	Ethanolamide		XZQKRCUYLKDPEK-BPVVGZHASA-N
15-HEPE	53480357		C20:5n3	R-OH	HMDB10209	UDXLGBLAJBYLSZ-XBCQTNLFSA-N
12-HEPE	10041593		C20:5n3	R-OH	HMDB10202	MCRJLMXYVFDXLS-QGQBRVLBSA-N
9-HEPE	5283187		C20:5n3	R-OH	HMDB60053	OXOPDAZWPWFJEW-FPRWAWDYSA-N
5-HEPE	6439678		C20:5n3	R-OH	HMDB05081	FTAGQROYQYQRHF-FCWZHQICSA-N
17-HDoHE	6439179		C22:6n3	R-OH	HMDB10213	SWTYBBUBEPPYCX-VIIQGJSXSA-N
14-HDoHE	11566378		C22:6n3	R-OH	HMDB60044	ZNEBXONKCYFJAF-BGKMTWLOSA-N
4-HDoHE	53394255		C22:6n3	R-OH	HMDB60049	IFRKCNPQVIJFAQ-JGDWKEERSA-N
9_10-EpO	15868		C18:1n9	Epox		IMYZYCNQZDBZBQ-UHFFFAOYSA-N
12(13)-EpOME	5356421		C18:2n6	Epox	HMDB04702	CCPPLLJZDQAOHD-FLIBITNWSA-N
9(10)-EpOME	5283018		C18:2n6	Epox		FBUKMFOXMZRGRB-JXMROGBWSA-N
15(16)-EpODE	16061062		C18:3n3	Epox	HMDB10206	HKSDVVJONLXYKL-OHPMOLHNSA-N
12(13)-EpODE	16061061		C18:3n3	Epox	HMDB10200	BKKGUKSHPCTUGE-OOHFSOINSA-N
9(10)-EpODE	16061060		C18:3n3	Epox	HMDB10220	JTEGNNHWOIJBJZ-ZJSQCTGTSA-N
14(15)-EpETrE	11954058	C14813	C20:4n6	Epox	HMDB04693	WLMZMBKVRPUYIG-LTCHCNGXSA-N
11(12)-EpETrE	53480479		C20:4n6	Epox	HMDB10409	DXOYQVHGIODESM-IQCOFVSKSA-N
8(9)-EpETrE	5283203	C14769	C20:4n6	Epox	HMDB02232	DBWQSCSXHFNTMO-TYAUOURKSA-N
20-HETE	5283157	C14748	C20:4n6	R-OH	HMDB05998	NNDIXBJHNLFJJP-DTLRTWKJSA-N
11(12)-EpETE	16061087		C20:5n3	Epox		QHOKDYBJJBDJGY-BVILWSOJSA-N
19(20)-EpDPE	11631565		C22:6n3	Epox	HMDB13620	OSXOPUBJJDUAOJ-MBYQGORISA-N
12(13)-Ep-9-KODE	5283007		C18:2n6	Epox,R=O	HMDB13623	RCMABBHQYMBYKV-BUHFOSPRSA-N
13-KODE	6446027	C14765	C18:2n6	R=O	HMDB04668	JHXAZBBVQSRKJR-BSZOFBHHSA-N
9-KODE	9839084	C14766	C18:2n6	R=O	HMDB04669	LUZSWWYKKLTDHU-ZJHFMPGASA-N
15-KETE	5280701	C04577	C20:4n6	R=O	HMDB10210	YGJTUEISKATQSM-USWFWKISSA-N
5-KETE	5283159	C14732	C20:4n6	R=O	HMDB10217	MEASLHGILYBXFO-XTDASVJISA-N
METABOLITES_END
#END