#METABOLOMICS WORKBENCH hormel101_20180416_091434_mwtab.txt DATATRACK_ID:1381 STUDY_ID:ST000971 ANALYSIS_ID:AN001591 PROJECT_ID:PR000659
VERSION             	1
CREATED_ON             	May 21, 2018, 3:01 pm
#PROJECT
PR:PROJECT_TITLE                 	Mayo Pilot and Feasibility: The Influence of Sugar, Artificial Sweeteners, and
PR:PROJECT_TITLE                 	the Microbiome on Metabolism
PR:PROJECT_SUMMARY               	As diabetes becomes a growing heath concern, afflicting nearly 25.8 million
PR:PROJECT_SUMMARY               	people in the United States and nearly 220 million people worldwide, there has
PR:PROJECT_SUMMARY               	been an increased awareness of environmental factors like diet that are
PR:PROJECT_SUMMARY               	contributing to the disease. In diabetic patients, a major causal factor
PR:PROJECT_SUMMARY               	contributing to progression of the disease is hyperglycemia, although the
PR:PROJECT_SUMMARY               	underlying mechanisms by which hyperglycemia impairs homeostatic processes are
PR:PROJECT_SUMMARY               	not well understood. While we know that early intensive glycemic control reduces
PR:PROJECT_SUMMARY               	the risk of cardiovascular complications in humans and rodent models, there is a
PR:PROJECT_SUMMARY               	large gap in studies of the etiology of hyperglycemia-induced alterations in the
PR:PROJECT_SUMMARY               	disease. To combat high sugar diets that could contribute to diabetes and
PR:PROJECT_SUMMARY               	subsequent hyperglycemia, non-caloric artificial sweeteners have become one of
PR:PROJECT_SUMMARY               	the most utilized food additives worldwide due to their consideration as a low
PR:PROJECT_SUMMARY               	caloric substitute. However, supporting scientific data as to the safety of
PR:PROJECT_SUMMARY               	these non-caloric artificial sweeteners is limited and controversial. The
PR:PROJECT_SUMMARY               	negative implications of consuming a high sugar diet on overall health have long
PR:PROJECT_SUMMARY               	been linked to diabetes, obesity, and resulting systemic health problems;
PR:PROJECT_SUMMARY               	however, it was not until recently that the negative impact of consuming
PR:PROJECT_SUMMARY               	artificial sweeteners in the place of sugar had been increasingly recognized.
PR:PROJECT_SUMMARY               	Recent evidence also suggests that a diet rich in artificial sweeteners can
PR:PROJECT_SUMMARY               	induce glucose intolerance through the alteration of the gut microbiome. We
PR:PROJECT_SUMMARY               	hypothesize that a specific subset of plasma metabolites are generated as a
PR:PROJECT_SUMMARY               	result from a diet rich in commonly used artificial sweeteners and their
PR:PROJECT_SUMMARY               	subsequent processing by the gut microbiome, which could ultimately lead to
PR:PROJECT_SUMMARY               	impaired glycemic control and negative physiological health outcomes. To test
PR:PROJECT_SUMMARY               	this hypothesis this study will 1) administer a diet high in glucose, fructose,
PR:PROJECT_SUMMARY               	and 4 common artificial sweeteners separately to rats followed by a plasma
PR:PROJECT_SUMMARY               	metabolic analysis (AIM 1) and 2) treat the gut microbiota with antibiotics in
PR:PROJECT_SUMMARY               	these animals to observe how alterations of the microbiome influence the plasma
PR:PROJECT_SUMMARY               	metabolic profile in animals receiving the altered diets (AIM 2). The resulting
PR:PROJECT_SUMMARY               	data will give us insights into the influence of high sugar and artificial
PR:PROJECT_SUMMARY               	sweetener diets on homeostatic metabolic processes and dive into the symbiotic
PR:PROJECT_SUMMARY               	relationship of the gut microbiome with this process. This data will provide
PR:PROJECT_SUMMARY               	crucial insights into the dietary use of artificial sweeteners in the
PR:PROJECT_SUMMARY               	replacement of sugars and how it alters metabolic pathways that could
PR:PROJECT_SUMMARY               	potentially lead to altered states of obesity, diabetes, and cardiovascular
PR:PROJECT_SUMMARY               	disease.
PR:INSTITUTE                     	Mayo Clinic
PR:LAST_NAME                     	Hoffmann
PR:FIRST_NAME                    	Brian
PR:ADDRESS                       	8701 Watertown Plank Road Milwaukee, WI 53226
PR:EMAIL                         	bhoffmann@mcw.edu
PR:PHONE                         	414-955-8671
#STUDY
ST:STUDY_TITLE                   	The Influence of Sugar, Artificial Sweeteners, and the Microbiome on Rodent
ST:STUDY_TITLE                   	Large Scale Profiling (part V)
ST:STUDY_SUMMARY                 	Large scale profiling of rodents treated with diets rich in commonly used
ST:STUDY_SUMMARY                 	artifically sweeteners will be assessed in this study. We hypothesized that a
ST:STUDY_SUMMARY                 	specific subset of plasma metabolites are generated as a result from a diet rich
ST:STUDY_SUMMARY                 	in commonly used artificial sweeteners and their subsequent processing by the
ST:STUDY_SUMMARY                 	gut microbiome, which could ultimately lead to impaired glycemic control and
ST:STUDY_SUMMARY                 	negative physiological health outcomes. To test this hypothesis we administered
ST:STUDY_SUMMARY                 	normal, high glucose, fructose, aspartame, and acesulfame potassium diets to
ST:STUDY_SUMMARY                 	rats for 3 weeks, followed by a plasma collection through cardiac puncture and
ST:STUDY_SUMMARY                 	metabolic analysis. We also treated the gut microbiota with in rats with the
ST:STUDY_SUMMARY                 	same diets plus bacitracin/streptomycin to observe how alterations of the
ST:STUDY_SUMMARY                 	microbiome influence the plasma metabolic profile in these animals. The
ST:STUDY_SUMMARY                 	resulting data will give us insights into the influence of high sugar and
ST:STUDY_SUMMARY                 	artificial sweetener diets on homeostatic metabolic processes and dive into the
ST:STUDY_SUMMARY                 	symbiotic relationship of the gut microbiome with this process.
ST:INSTITUTE                     	Mayo Clinic
ST:LAST_NAME                     	Hoffmann
ST:FIRST_NAME                    	Brian
ST:ADDRESS                       	8701 Watertown Plank Road Milwaukee, WI 53226
ST:EMAIL                         	bhoffmann@mcw.edu
ST:PHONE                         	414-955-8671
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Rattus norvegicus
SU:TAXONOMY_ID                   	10116
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	ms6571-1	nC18-25mar17-001	Group:Group 7	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-2	nC18-25mar17-002	Group:Group 6	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-3	nC18-25mar17-003	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-4	nC18-25mar17-004	Group:Group 3	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-5	nC18-25mar17-005	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-6	nC18-25mar17-006	Group:Group 1	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-7	nC18-25mar17-007	Group:Group 1	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-8	nC18-25mar17-008	Group:Group 8	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-9	nC18-25mar17-009	Group:Group 4	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-10	nC18-25mar17-010	Group:Group 2	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-11	nC18-25mar17-011	Group:Group 7	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-12	nC18-25mar17-012	Group:Group 1	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-13	nC18-25mar17-013	Group:Group 6	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-14	nC18-25mar17-014	Group:Group 4	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-15	nC18-25mar17-015	Group:Group 5	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-16	nC18-25mar17-016	Group:Group 5	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-17	nC18-25mar17-017	Group:Group 5	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-18	nC18-25mar17-018	Group:Group 6	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-19	nC18-25mar17-019	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-20	nC18-25mar17-020	Group:Group 2	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-21	nC18-25mar17-021	Group:Group 2	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-22	nC18-25mar17-022	Group:Group 7	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-23	nC18-25mar17-023	Group:Group 5	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-24	nC18-25mar17-024	Group:Group 8	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-25	nC18-25mar17-025	Group:Group 6	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-26	nC18-25mar17-026	Group:Group 5	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-27	nC18-25mar17-027	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-28	nC18-25mar17-028	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-29	nC18-25mar17-029	Group:Group 4	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-30	nC18-25mar17-030	Group:Group 2	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-31	nC18-26mar17-031	Group:Group 1	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-32	nC18-26mar17-032	Group:Group 2	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-33	nC18-26mar17-033	Group:Group 4	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-34	nC18-26mar17-034	Group:Group 4	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-35	nC18-26mar17-035	Group:Group 7	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-36	nC18-26mar17-036	Group:Group 8	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-37	nC18-26mar17-037	Group:Group 8	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-38	nC18-26mar17-038	Group:Group 1	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-39	nC18-26mar17-039	Group:Group 1	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-40	nC18-26mar17-040	Group:Group 8	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-41	nC18-26mar17-041	Group:Group 6	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-42	nC18-26mar17-042	Group:Group 2	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-43	nC18-26mar17-043	Group:Group 5	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-44	nC18-26mar17-044	Group:Group 6	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-45	nC18-26mar17-045	Group:Group 8	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-46	nC18-26mar17-046	Group:Group 7	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-47	nC18-26mar17-047	Group:Group 4	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-48	nC18-26mar17-048	Group:Group 7	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-49	nC18-26mar17-049	Group:Group 9	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-50	nC18-26mar17-050	Group:Group 10	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-51	nC18-26mar17-051	Group:Group 10	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-52	nC18-26mar17-052	Group:Group 9	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-53	nC18-26mar17-053	Group:Group 10	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-54	nC18-26mar17-054	Group:Group 10	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-55	nC18-26mar17-055	Group:Group 9	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-56	nC18-26mar17-056	Group:Group 9	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-57	nC18-26mar17-057	Group:Group 10	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-58	nC18-26mar17-058	Group:Group 9	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-59	nC18-26mar17-059	Group:Group 10	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-60	nC18-26mar17-060	Group:Group 10	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NC18
SUBJECT_SAMPLE_FACTORS           	ms6571-1	nhilic-24mar17-001	Group:Group 7	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-2	nhilic-24mar17-002	Group:Group 6	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-3	nhilic-24mar17-003	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-4	nhilic-24mar17-004	Group:Group 3	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-5	nhilic-24mar17-005	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-6	nhilic-24mar17-006	Group:Group 1	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-7	nhilic-24mar17-007	Group:Group 1	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-8	nhilic-24mar17-008	Group:Group 8	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-9	nhilic-24mar17-009	Group:Group 4	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-10	nhilic-24mar17-010	Group:Group 2	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-11	nhilic-24mar17-011	Group:Group 7	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-12	nhilic-24mar17-012	Group:Group 1	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-13	nhilic-24mar17-013	Group:Group 6	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-14	nhilic-24mar17-014	Group:Group 4	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-15	nhilic-24mar17-015	Group:Group 5	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-16	nhilic-24mar17-016	Group:Group 5	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-17	nhilic-24mar17-017	Group:Group 5	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-18	nhilic-24mar17-018	Group:Group 6	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-19	nhilic-24mar17-019	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-20	nhilic-24mar17-020	Group:Group 2	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-21	nhilic-24mar17-021	Group:Group 2	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-22	nhilic-24mar17-022	Group:Group 7	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-23	nhilic-24mar17-023	Group:Group 5	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-24	nhilic-24mar17-024	Group:Group 8	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-25	nhilic-24mar17-025	Group:Group 6	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-26	nhilic-24mar17-026	Group:Group 5	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-27	nhilic-24mar17-027	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-28	nhilic-24mar17-028	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-29	nhilic-24mar17-029	Group:Group 4	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-30	nhilic-24mar17-030	Group:Group 2	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-31	nhilic-25mar17-031	Group:Group 1	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-32	nhilic-25mar17-032	Group:Group 2	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-33	nhilic-25mar17-033	Group:Group 4	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-34	nhilic-25mar17-034	Group:Group 4	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-35	nhilic-25mar17-035	Group:Group 7	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-36	nhilic-25mar17-036	Group:Group 8	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-37	nhilic-25mar17-037	Group:Group 8	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-38	nhilic-25mar17-038	Group:Group 1	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-39	nhilic-25mar17-039	Group:Group 1	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-40	nhilic-25mar17-040	Group:Group 8	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-41	nhilic-25mar17-041	Group:Group 6	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-42	nhilic-25mar17-042	Group:Group 2	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-43	nhilic-25mar17-043	Group:Group 5	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-44	nhilic-25mar17-044	Group:Group 6	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-45	nhilic-25mar17-045	Group:Group 8	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-46	nhilic-25mar17-046	Group:Group 7	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-47	nhilic-25mar17-047	Group:Group 4	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-48	nhilic-25mar17-048	Group:Group 7	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-49	nhilic-25mar17-049	Group:Group 9	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-50	nhilic-25mar17-050	Group:Group 10	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-51	nhilic-25mar17-051	Group:Group 10	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-52	nhilic-25mar17-052	Group:Group 9	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-53	nhilic-25mar17-053	Group:Group 10	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-54	nhilic-25mar17-054	Group:Group 10	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-55	nhilic-25mar17-055	Group:Group 9	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-56	nhilic-25mar17-056	Group:Group 9	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-57	nhilic-25mar17-057	Group:Group 10	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-58	nhilic-25mar17-058	Group:Group 9	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-59	nhilic-25mar17-059	Group:Group 10	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-60	nhilic-25mar17-060	Group:Group 10	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-1	pC18-22mar17-001	Group:Group 7	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-2	pC18-22mar17-002	Group:Group 6	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-3	pC18-22mar17-003	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-4	pC18-22mar17-004	Group:Group 3	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-5	pC18-22mar17-005	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-6	pC18-22mar17-006	Group:Group 1	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-7	pC18-22mar17-007	Group:Group 1	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-8	pC18-22mar17-008	Group:Group 8	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-9	pC18-22mar17-009	Group:Group 4	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-10	pC18-22mar17-010	Group:Group 2	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-11	pC18-22mar17-011	Group:Group 7	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-12	pC18-22mar17-012	Group:Group 1	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-13	pC18-22mar17-013	Group:Group 6	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-14	pC18-22mar17-014	Group:Group 4	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-15	pC18-22mar17-015	Group:Group 5	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-16	pC18-22mar17-016	Group:Group 5	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-17	pC18-22mar17-017	Group:Group 5	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-18	pC18-22mar17-018	Group:Group 6	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-19	pC18-22mar17-019	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-20	pC18-22mar17-020	Group:Group 2	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-21	pC18-22mar17-021	Group:Group 2	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-22	pC18-22mar17-022	Group:Group 7	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-23	pC18-22mar17-023	Group:Group 5	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-24	pC18-22mar17-024	Group:Group 8	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-25	pC18-22mar17-025	Group:Group 6	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-26	pC18-22mar17-026	Group:Group 5	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-27	pC18-22mar17-027	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-28	pC18-22mar17-028	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-29	pC18-22mar17-029	Group:Group 4	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-30	pC18-22mar17-030	Group:Group 2	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-31	pC18-27mar17-031	Group:Group 1	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-32	pC18-27mar17-032	Group:Group 2	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-33	pC18-27mar17-033	Group:Group 4	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-34	pC18-27mar17-034	Group:Group 4	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-35	pC18-27mar17-035	Group:Group 7	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-36	pC18-27mar17-036	Group:Group 8	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-37	pC18-27mar17-037	Group:Group 8	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-38	pC18-27mar17-038	Group:Group 1	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-39	pC18-27mar17-039	Group:Group 1	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-40	pC18-27mar17-040	Group:Group 8	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-41	pC18-27mar17-041	Group:Group 6	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-42	pC18-27mar17-042	Group:Group 2	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-43	pC18-27mar17-043	Group:Group 5	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-44	pC18-27mar17-044	Group:Group 6	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-45	pC18-27mar17-045	Group:Group 8	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-46	pC18-27mar17-046	Group:Group 7	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-47	pC18-27mar17-047	Group:Group 4	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-48	pC18-27mar17-048	Group:Group 7	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-49	pC18-27mar17-049	Group:Group 9	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-50	pC18-27mar17-050	Group:Group 10	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-51	pC18-27mar17-051	Group:Group 10	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-52	pC18-27mar17-052	Group:Group 9	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-53	pC18-27mar17-053	Group:Group 10	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-54	pC18-27mar17-054	Group:Group 10	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-55	pC18-27mar17-055	Group:Group 9	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-56	pC18-27mar17-056	Group:Group 9	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-57	pC18-27mar17-057	Group:Group 10	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-58	pC18-27mar17-058	Group:Group 9	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-59	pC18-27mar17-059	Group:Group 10	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-60	pC18-27mar17-060	Group:Group 10	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PC18
SUBJECT_SAMPLE_FACTORS           	ms6571-1	philic-21mar17-001-r001	Group:Group 7	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-1	philic-21mar17-001-r002	Group:Group 7	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-2	philic-21mar17-002-r001	Group:Group 6	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-2	philic-21mar17-002-r002	Group:Group 6	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-3	philic-21mar17-003-r001	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-3	philic-21mar17-003-r002	Group:Group 3	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-4	philic-21mar17-004-r001	Group:Group 3	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-4	philic-21mar17-004-r002	Group:Group 3	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-5	philic-21mar17-005-r001	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-5	philic-21mar17-005-r002	Group:Group 3	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-6	philic-21mar17-006-r001	Group:Group 1	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-6	philic-21mar17-006-r002	Group:Group 1	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-7	philic-21mar17-007-r001	Group:Group 1	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-7	philic-21mar17-007-r002	Group:Group 1	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-8	philic-21mar17-008-r001	Group:Group 8	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-8	philic-21mar17-008-r002	Group:Group 8	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-9	philic-21mar17-009-r001	Group:Group 4	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-9	philic-21mar17-009-r002	Group:Group 4	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-10	philic-21mar17-010-r001	Group:Group 2	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-10	philic-21mar17-010-r002	Group:Group 2	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-11	philic-21mar17-011-r001	Group:Group 7	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-11	philic-21mar17-011-r002	Group:Group 7	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-12	philic-21mar17-012-r001	Group:Group 1	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-12	philic-21mar17-012-r002	Group:Group 1	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-13	philic-21mar17-013-r001	Group:Group 6	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-13	philic-21mar17-013-r002	Group:Group 6	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-14	philic-21mar17-014-r001	Group:Group 4	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-14	philic-21mar17-014-r002	Group:Group 4	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-15	philic-21mar17-015-r001	Group:Group 5	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-15	philic-21mar17-015-r002	Group:Group 5	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-16	philic-21mar17-016-r001	Group:Group 5	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-16	philic-21mar17-016-r002	Group:Group 5	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-17	philic-21mar17-017-r001	Group:Group 5	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-17	philic-21mar17-017-r002	Group:Group 5	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-18	philic-21mar17-018-r001	Group:Group 6	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-18	philic-21mar17-018-r002	Group:Group 6	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-19	philic-21mar17-019-r001	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-19	philic-21mar17-019-r002	Group:Group 3	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-20	philic-21mar17-020-r001	Group:Group 2	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-20	philic-21mar17-020-r002	Group:Group 2	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-21	philic-21mar17-021-r001	Group:Group 2	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-21	philic-21mar17-021-r002	Group:Group 2	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-22	philic-21mar17-022-r001	Group:Group 7	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-22	philic-21mar17-022-r002	Group:Group 7	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-23	philic-21mar17-023-r001	Group:Group 5	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-23	philic-21mar17-023-r002	Group:Group 5	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-24	philic-21mar17-024-r001	Group:Group 8	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-24	philic-21mar17-024-r002	Group:Group 8	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-25	philic-21mar17-025-r001	Group:Group 6	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-25	philic-21mar17-025-r002	Group:Group 6	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-26	philic-21mar17-026-r001	Group:Group 5	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-26	philic-21mar17-026-r002	Group:Group 5	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-27	philic-21mar17-027-r001	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-27	philic-21mar17-027-r002	Group:Group 3	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-28	philic-21mar17-028-r001	Group:Group 3	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-28	philic-21mar17-028-r002	Group:Group 3	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-29	philic-21mar17-029-r001	Group:Group 4	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-29	philic-21mar17-029-r002	Group:Group 4	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-30	philic-21mar17-030-r001	Group:Group 2	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-30	philic-21mar17-030-r002	Group:Group 2	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-31	philic-22mar17-031-r001	Group:Group 1	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-31	philic-22mar17-031-r002	Group:Group 1	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-32	philic-22mar17-032-r001	Group:Group 2	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-32	philic-22mar17-032-r002	Group:Group 2	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-33	philic-22mar17-033-r001	Group:Group 4	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-33	philic-22mar17-033-r002	Group:Group 4	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-34	philic-22mar17-034-r001	Group:Group 4	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-34	philic-22mar17-034-r002	Group:Group 4	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-35	philic-22mar17-035-r001	Group:Group 7	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-35	philic-22mar17-035-r002	Group:Group 7	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-36	philic-22mar17-036-r001	Group:Group 8	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-36	philic-22mar17-036-r002	Group:Group 8	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-37	philic-22mar17-037-r001	Group:Group 8	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-37	philic-22mar17-037-r002	Group:Group 8	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-38	philic-22mar17-038-r001	Group:Group 1	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-38	philic-22mar17-038-r002	Group:Group 1	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-39	philic-22mar17-039-r001	Group:Group 1	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-39	philic-22mar17-039-r002	Group:Group 1	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-40	philic-22mar17-040-r001	Group:Group 8	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-40	philic-22mar17-040-r002	Group:Group 8	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-41	philic-22mar17-041-r001	Group:Group 6	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-41	philic-22mar17-041-r002	Group:Group 6	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-42	philic-22mar17-042-r001	Group:Group 2	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-42	philic-22mar17-042-r002	Group:Group 2	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-43	philic-22mar17-043-r001	Group:Group 5	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-43	philic-22mar17-043-r002	Group:Group 5	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-44	philic-22mar17-044-r001	Group:Group 6	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-44	philic-22mar17-044-r002	Group:Group 6	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-45	philic-22mar17-045-r001	Group:Group 8	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-45	philic-22mar17-045-r002	Group:Group 8	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-46	philic-22mar17-046-r001	Group:Group 7	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-46	philic-22mar17-046-r002	Group:Group 7	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-47	philic-22mar17-047-r001	Group:Group 4	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-47	philic-22mar17-047-r002	Group:Group 4	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-48	philic-22mar17-048-r001	Group:Group 7	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-48	philic-22mar17-048-r002	Group:Group 7	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-49	philic-22mar17-049-r001	Group:Group 9	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-49	philic-22mar17-049-r002	Group:Group 9	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-50	philic-22mar17-050-r001	Group:Group 10	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-50	philic-22mar17-050-r002	Group:Group 10	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-51	philic-22mar17-051-r001	Group:Group 10	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-51	philic-22mar17-051-r002	Group:Group 10	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-52	philic-22mar17-052-r001	Group:Group 9	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-52	philic-22mar17-052-r002	Group:Group 9	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-53	philic-22mar17-053-r001	Group:Group 10	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-53	philic-22mar17-053-r002	Group:Group 10	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-54	philic-22mar17-054-r001	Group:Group 10	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-54	philic-22mar17-054-r002	Group:Group 10	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-55	philic-22mar17-055-r001	Group:Group 9	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-55	philic-22mar17-055-r002	Group:Group 9	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-56	philic-22mar17-056-r001	Group:Group 9	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-56	philic-22mar17-056-r002	Group:Group 9	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-57	philic-22mar17-057-r001	Group:Group 10	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-57	philic-22mar17-057-r002	Group:Group 10	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-58	philic-22mar17-058-r001	Group:Group 9	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-58	philic-22mar17-058-r002	Group:Group 9	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-59	philic-22mar17-059-r001	Group:Group 10	reps=1; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-59	philic-22mar17-059-r002	Group:Group 10	reps=2; Gender=M; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-60	philic-22mar17-060-r001	Group:Group 10	reps=1; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
SUBJECT_SAMPLE_FACTORS           	ms6571-60	philic-22mar17-060-r002	Group:Group 10	reps=2; Gender=F; Sample=Plasma; Specied=Rat; chromatography=PCHILIC
#COLLECTION
CO:COLLECTION_SUMMARY            	"Samples were collected by cardiac puncture and plasma was collected following
CO:COLLECTION_SUMMARY            	standard centrifugation steps. Immediately following the samples were separated
CO:COLLECTION_SUMMARY            	into 500 uL aliquots in 600 uL tubes and frozen in liquid nitrogen.
CO:SAMPLE_TYPE                   	Blood (plasma)
#TREATMENT
TR:TREATMENT_SUMMARY             	"We administered normal, high glucose, aspartame, and acesulfame potassium diets
TR:TREATMENT_SUMMARY             	to rats for 3 weeks, followed by a plasma collection through cardiac puncture
TR:TREATMENT_SUMMARY             	and metabolic analysis (Group 1-4 samples). We also treated the gut microbiota
TR:TREATMENT_SUMMARY             	with in rats with the same diets plus bacitracin/streptomycin to observe how
TR:TREATMENT_SUMMARY             	alterations of the microbiome influence the plasma metabolic profile in these
TR:TREATMENT_SUMMARY             	animals (Groups 5-8). The samples here contain the fructose diet group (Group
TR:TREATMENT_SUMMARY             	9). We also treated the gut microbiota with in rats with the same diets plus
TR:TREATMENT_SUMMARY             	bacitracin/streptomycin to observe how alterations of the microbiome influence
TR:TREATMENT_SUMMARY             	the plasma metabolic profile in these animals (Groups 5-8). The samples here
TR:TREATMENT_SUMMARY             	contain the fructose diet group with antibiotic (Group 10). For the gut
TR:TREATMENT_SUMMARY             	microbiota experiment, during the last 10 days of the diet subsets of all groups
TR:TREATMENT_SUMMARY             	will have bacitracin and streptomycin (B/S) provided in their drinking water
TR:TREATMENT_SUMMARY             	(0.5g/250 mL). The resulting data will give us insights into the influence of
TR:TREATMENT_SUMMARY             	high sugar and artificial sweetener diets on homeostatic metabolic processes and
TR:TREATMENT_SUMMARY             	dive into the symbiotic relationship of the gut microbiome with this process.
TR:TREATMENT_SUMMARY             	Group1 = normal diet Group2 = high glucose diet Group3 = aspartame diet Group4 =
TR:TREATMENT_SUMMARY             	acesulfame potassium diet Group5 = rat gut microbiota normal diet + antibotics
TR:TREATMENT_SUMMARY             	Group6 = rat gut microbiota high glucose diet + antibotics Group7 = rat gut
TR:TREATMENT_SUMMARY             	microbiota aspartame diet + antibotics Group8 = rat gut acesulfame potassium
TR:TREATMENT_SUMMARY             	diet + antibotics Group9 = fructose diet Group10 = rat gut fructose diet +
TR:TREATMENT_SUMMARY             	antibotics"
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	large scale profiling
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity
CH:COLUMN_NAME                   	Waters Acquity HSS C18 (150 x 2.1mm, 1.8um)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:MS_COMMENTS                   	-
MS:INSTRUMENT_NAME               	Agilent 6550 QTOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_RESULTS_FILE               	ST000971_AN001591_Results.txt	UNITS:intensity
#END