#METABOLOMICS WORKBENCH hormel101_20180710_080600_mwtab.txt DATATRACK_ID:1438 STUDY_ID:ST001003 ANALYSIS_ID:AN001642 PROJECT_ID:PR000678
VERSION             	1
CREATED_ON             	July 11, 2018, 4:16 pm
#PROJECT
PR:PROJECT_TITLE                 	Mayo Pilot and Feasibility: A pilot study of urine metabolomics towards an
PR:PROJECT_TITLE                 	outpatient estimate of circadian phase
PR:PROJECT_SUMMARY               	"Although the effectiveness of several therapeutic interventions depend
PR:PROJECT_SUMMARY               	critically on their timing with respect to circadian phase, including the timing
PR:PROJECT_SUMMARY               	of light therapy for circadian rhythm sleep disorders, medications for high
PR:PROJECT_SUMMARY               	blood pressure, and chemotherapy treatments for cancer, no clinical test is
PR:PROJECT_SUMMARY               	available to reliably measure circadian phase rapidly, inexpensively, and
PR:PROJECT_SUMMARY               	non-invasively. This project will therefore provide the essential first steps
PR:PROJECT_SUMMARY               	toward the development and validation of a clinical test to estimate circadian
PR:PROJECT_SUMMARY               	phase from a single urine void via the identification of multiple rhythmic
PR:PROJECT_SUMMARY               	metabolites in urine using untargeted metabolomic profiling methods. Current
PR:PROJECT_SUMMARY               	methods to assess circadian phase in urine require serial measurement of a
PR:PROJECT_SUMMARY               	single compound (e.g., 6-sulphatoxymelatonin, the urinary metabolite of
PR:PROJECT_SUMMARY               	melatonin) over a 24- to 48-hour sampling window. Our approach proposes to
PR:PROJECT_SUMMARY               	assess many compounds in one sample to estimate circadian phase, based on the
PR:PROJECT_SUMMARY               	phase relationships across multiple parameters, an approach that is supported by
PR:PROJECT_SUMMARY               	our theoretical modeling framework. Using the untargeted metabolomics profiling
PR:PROJECT_SUMMARY               	services offered by the Mayo Clinic Metabolomics Resource Core, we will examine
PR:PROJECT_SUMMARY               	the 48-hour profiles of ~300 metabolites identified from urine samples collected
PR:PROJECT_SUMMARY               	in a randomly selected pilot sample of 12 healthy young volunteers (from >200
PR:PROJECT_SUMMARY               	subjects) studied on an inpatient laboratory protocol that included both an
PR:PROJECT_SUMMARY               	ambulatory condition (i.e., habitual sleep-wake times under ordinary room light)
PR:PROJECT_SUMMARY               	and a constant routine procedure, the gold standard method for assessing
PR:PROJECT_SUMMARY               	circadian rhythms (i.e., 50-hour period during which subjects remain awake in a
PR:PROJECT_SUMMARY               	semi-recumbent posture in bed under dim light with equicaloric snacks served
PR:PROJECT_SUMMARY               	hourly). Cosinor analysis will be employed to determine which identified
PR:PROJECT_SUMMARY               	metabolites exhibit circadian rhythmicity, and comparisons between ambulatory
PR:PROJECT_SUMMARY               	and constantroutine conditions will further identify which metabolites are
PR:PROJECT_SUMMARY               	influenced by external factors such as sleep, meal timing, light, and posture.
PR:PROJECT_SUMMARY               	Finally, we will employ our theoretical modeling framework to estimate circadian
PR:PROJECT_SUMMARY               	phase from a single urine void using the concentration ratios of multiple
PR:PROJECT_SUMMARY               	metabolites that exhibit reliable and robust circadian rhythmicity. The accuracy
PR:PROJECT_SUMMARY               	of estimated circadian phase will be determined by comparison to actual
PR:PROJECT_SUMMARY               	circadian phase as defined by the peak of the 6-sulphatoxymelatonin rhythm. Once
PR:PROJECT_SUMMARY               	this approach has been established in a pilot set of subjects, future studies
PR:PROJECT_SUMMARY               	will focus on validation and testing of this approach in other data from our
PR:PROJECT_SUMMARY               	repository, including healthy young volunteers who have undergone rapid phase
PR:PROJECT_SUMMARY               	shift due to changes in sleep-wake schedule (i.e., simulated shift work) or in
PR:PROJECT_SUMMARY               	response to bright light exposure; patients with insomnia, who exhibit an 8-hour
PR:PROJECT_SUMMARY               	range in circadian phase; and blind participants without light perception, who
PR:PROJECT_SUMMARY               	exhibit non-entrained rhythms. Future studies will also test the efficacy of
PR:PROJECT_SUMMARY               	this method to a priori estimate circadian phase in patient populations that may
PR:PROJECT_SUMMARY               	benefit from improvements in circadian timing of treatment. The current proposal
PR:PROJECT_SUMMARY               	therefore represents the first essential step in developing a tool that can
PR:PROJECT_SUMMARY               	revolutionize medicine by adding an accurate measure of internal time –
PR:PROJECT_SUMMARY               	circadian medicine – into standard clinical practice.
PR:INSTITUTE                     	Mayo Clinic
PR:LAST_NAME                     	Hilaire
PR:FIRST_NAME                    	Melissa
PR:ADDRESS                       	221 Longwood Avenue, Suite BL438 Boston, Massachusetts 02115
PR:EMAIL                         	msthilaire@rics.bwh.harvard.edu
PR:PHONE                         	617-732-4013
#STUDY
ST:STUDY_TITLE                   	A pilot study of urine metabolomics in a female subject towards an outpatient
ST:STUDY_TITLE                   	estimate of circadian phase
ST:STUDY_SUMMARY                 	Serial urine samples were collected at each void (approximately every 3 hours)
ST:STUDY_SUMMARY                 	from subjects during a 6-day inpatient protocol. The total volume of each sample
ST:STUDY_SUMMARY                 	was measured, and then 5 mL was aliquoted into a 7 mL tube and delivered on ice
ST:STUDY_SUMMARY                 	to the processing lab, where the samples were then stored at -80 degrees. At the
ST:STUDY_SUMMARY                 	end of the study, samples were transported (~2 blocks) from the processing lab
ST:STUDY_SUMMARY                 	to our -80 freezer for storage. The samples being sent represent samples from
ST:STUDY_SUMMARY                 	one female subject. This subject spent 6 days in the lab: 3 baseline days where
ST:STUDY_SUMMARY                 	the subject slept for 8 hours at night (at habitual times as determined during
ST:STUDY_SUMMARY                 	the screening period) and 16 hours of ambulatory wake in ambient light, followed
ST:STUDY_SUMMARY                 	by 50 hours of continuous wakefulness in which the subject was kept in a
ST:STUDY_SUMMARY                 	semi-recumbent position in bed under dim light and fed hourly isocaloric snacks
ST:STUDY_SUMMARY                 	(called a "constant routine"). We are requesting untargeted profiling of 25
ST:STUDY_SUMMARY                 	samples (sample #: 52919-52943) to determine how the concentrations of different
ST:STUDY_SUMMARY                 	metabolites vary across the 24-hour period, and specifically to compare this
ST:STUDY_SUMMARY                 	circadian variation in each metabolite during a 48-hour ambulatory period versus
ST:STUDY_SUMMARY                 	a 48-hour constant routine period.
ST:INSTITUTE                     	Mayo Clinic
ST:LAST_NAME                     	Hilaire
ST:FIRST_NAME                    	Melissa
ST:ADDRESS                       	221 Longwood Avenue, Suite BL438 Boston, Massachusetts 02115
ST:EMAIL                         	msthilaire@rics.bwh.harvard.edu
ST:PHONE                         	617-732-4013
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-001	Hour:11 | day:day1 | Group:Baseline	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-001	Hour:11 | day:day1 | Group:Baseline	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-001	Hour:11 | day:day1 | Group:Baseline	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-001	Hour:11 | day:day1 | Group:Baseline	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-003	Hour:13 | day:day1 | Group:Baseline	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-003	Hour:13 | day:day1 | Group:Baseline	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-003	Hour:13 | day:day1 | Group:Baseline	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-003	Hour:13 | day:day1 | Group:Baseline	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-005	Hour:16 | day:day1 | Group:Baseline	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-005	Hour:16 | day:day1 | Group:Baseline	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-005	Hour:16 | day:day1 | Group:Baseline	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-005	Hour:16 | day:day1 | Group:Baseline	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-007	Hour:19 | day:day1 | Group:Baseline	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-007	Hour:19 | day:day1 | Group:Baseline	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-007	Hour:19 | day:day1 | Group:Baseline	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-007	Hour:19 | day:day1 | Group:Baseline	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-009	Hour:22 | day:day1 | Group:Baseline	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-009	Hour:22 | day:day1 | Group:Baseline	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-009	Hour:22 | day:day1 | Group:Baseline	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-009	Hour:22 | day:day1 | Group:Baseline	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-011	Hour:8 | day:day2 | Group:Baseline	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-011	Hour:8 | day:day2 | Group:Baseline	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-011	Hour:8 | day:day2 | Group:Baseline	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-011	Hour:8 | day:day2 | Group:Baseline	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-013	Hour:11 | day:day2 | Group:Baseline	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-013	Hour:11 | day:day2 | Group:Baseline	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-013	Hour:11 | day:day2 | Group:Baseline	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-013	Hour:11 | day:day2 | Group:Baseline	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-015	Hour:13 | day:day2 | Group:Baseline	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-015	Hour:13 | day:day2 | Group:Baseline	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-015	Hour:13 | day:day2 | Group:Baseline	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-015	Hour:13 | day:day2 | Group:Baseline	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-017	Hour:16 | day:day2 | Group:Baseline	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-017	Hour:16 | day:day2 | Group:Baseline	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-017	Hour:16 | day:day2 | Group:Baseline	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-017	Hour:16 | day:day2 | Group:Baseline	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-019	Hour:18 | day:day2 | Group:Baseline	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-019	Hour:18 | day:day2 | Group:Baseline	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-019	Hour:18 | day:day2 | Group:Baseline	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-019	Hour:18 | day:day2 | Group:Baseline	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-021	Hour:20 | day:day2 | Group:Baseline	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-021	Hour:20 | day:day2 | Group:Baseline	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-021	Hour:20 | day:day2 | Group:Baseline	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-021	Hour:20 | day:day2 | Group:Baseline	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-023	Hour:22 | day:day2 | Group:Baseline	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-023	Hour:22 | day:day2 | Group:Baseline	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-023	Hour:22 | day:day2 | Group:Baseline	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-023	Hour:22 | day:day2 | Group:Baseline	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-002	Hour:7 | day:day2 | Group:CR	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-002	Hour:7 | day:day2 | Group:CR	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-002	Hour:7 | day:day2 | Group:CR	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-002	Hour:7 | day:day2 | Group:CR	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-004	Hour:10 | day:day2 | Group:CR	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-004	Hour:10 | day:day2 | Group:CR	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-004	Hour:10 | day:day2 | Group:CR	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-004	Hour:10 | day:day2 | Group:CR	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-006	Hour:13 | day:day2 | Group:CR	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-006	Hour:13 | day:day2 | Group:CR	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-006	Hour:13 | day:day2 | Group:CR	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-006	Hour:13 | day:day2 | Group:CR	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-008	Hour:16 | day:day2 | Group:CR	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-008	Hour:16 | day:day2 | Group:CR	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-008	Hour:16 | day:day2 | Group:CR	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-008	Hour:16 | day:day2 | Group:CR	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-010	Hour:19 | day:day2 | Group:CR	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-010	Hour:19 | day:day2 | Group:CR	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-010	Hour:19 | day:day2 | Group:CR	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-010	Hour:19 | day:day2 | Group:CR	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-012	Hour:22 | day:day2 | Group:CR	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-012	Hour:22 | day:day2 | Group:CR	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-012	Hour:22 | day:day2 | Group:CR	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-012	Hour:22 | day:day2 | Group:CR	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-014	Hour:6 | day:day3 | Group:CR	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-014	Hour:6 | day:day3 | Group:CR	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-014	Hour:6 | day:day3 | Group:CR	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-014	Hour:6 | day:day3 | Group:CR	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-016	Hour:10 | day:day3 | Group:CR	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-016	Hour:10 | day:day3 | Group:CR	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-016	Hour:10 | day:day3 | Group:CR	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-016	Hour:10 | day:day3 | Group:CR	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-018	Hour:13 | day:day3 | Group:CR	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-018	Hour:13 | day:day3 | Group:CR	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-018	Hour:13 | day:day3 | Group:CR	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-018	Hour:13 | day:day3 | Group:CR	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-020	Hour:16 | day:day3 | Group:CR	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-020	Hour:16 | day:day3 | Group:CR	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-020	Hour:16 | day:day3 | Group:CR	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-020	Hour:16 | day:day3 | Group:CR	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-022	Hour:19 | day:day3 | Group:CR	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-022	Hour:19 | day:day3 | Group:CR	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-022	Hour:19 | day:day3 | Group:CR	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-022	Hour:19 | day:day3 | Group:CR	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-024	Hour:- | day:day4 | Group:CR	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-024	Hour:- | day:day4 | Group:CR	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-024	Hour:- | day:day4 | Group:CR	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-024	Hour:- | day:day4 | Group:CR	Type=Urine; chromatography=nC18
SUBJECT_SAMPLE_FACTORS           	-	philic-13oct16-025	Hour:7 | day:day4 | Group:CR	Type=Urine; chromatography=philic
SUBJECT_SAMPLE_FACTORS           	-	nhilic-12oct16-025	Hour:7 | day:day4 | Group:CR	Type=Urine; chromatography=nhilic
SUBJECT_SAMPLE_FACTORS           	-	pC18-14oct16-025	Hour:7 | day:day4 | Group:CR	Type=Urine; chromatography=pC18
SUBJECT_SAMPLE_FACTORS           	-	nC18-18oct16-025	Hour:7 | day:day4 | Group:CR	Type=Urine; chromatography=nC18
#COLLECTION
CO:COLLECTION_SUMMARY            	Serial urine samples were collected at each void (approximately every 3 hours)
CO:COLLECTION_SUMMARY            	from subjects during a 6-day inpatient protocol. The total volume of each sample
CO:COLLECTION_SUMMARY            	was measured, and then 5 mL was aliquoted into a 7 mL tube and delivered on ice
CO:COLLECTION_SUMMARY            	to the processing lab, where the samples were then stored at -80 degrees. At the
CO:COLLECTION_SUMMARY            	end of the study, samples were transported (~2 blocks) from the processing lab
CO:COLLECTION_SUMMARY            	to our -80 freezer for storage.
CO:SAMPLE_TYPE                   	Urine
#TREATMENT
TR:TREATMENT_SUMMARY             	This subject (subject code: 3635A) spent 6 days in the lab: 3 baseline days
TR:TREATMENT_SUMMARY             	where the subject slept for 8 hours at night (at habitual times as determined
TR:TREATMENT_SUMMARY             	during the screening period) and 16 hours of ambulatory wake in ambient light,
TR:TREATMENT_SUMMARY             	followed by 50 hours of continuous wakefulness in which the subject was kept in
TR:TREATMENT_SUMMARY             	a semi-recumbent position in bed under dim light and fed hourly isocaloric
TR:TREATMENT_SUMMARY             	snacks (called a "constant routine"). In the study design, baseline and CR are
TR:TREATMENT_SUMMARY             	used to group the 3 day baseline days and constant routine days.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	large scale profiling
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity
CH:COLUMN_NAME                   	Waters Acquity BEH Amide (150 x 2.1mm, 1.7um)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent 6550 QTOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_RESULTS_FILE               	ST001003_AN001642_Results.txt	UNITS:intensity
#END