#METABOLOMICS WORKBENCH hormel101_20180813_071242 DATATRACK_ID:1472 STUDY_ID:ST001035 ANALYSIS_ID:AN001696 PROJECT_ID:PR000693
VERSION             	1
CREATED_ON             	August 15, 2018, 12:35 pm
#PROJECT
PR:PROJECT_TITLE                 	Mayo Pilot and Feasibility: The pathogenic NMO IgG dysregulates the astrocytic
PR:PROJECT_TITLE                 	glutamine-glutamate cycle: a metabolic basis for depression in NMO patients
PR:PROJECT_SUMMARY               	Neuromyelitis optica (NMO) is a disabling central nervous system (CNS)
PR:PROJECT_SUMMARY               	inflammatory disorder that involves a pathogenic autoantibody (NMO IgG) directed
PR:PROJECT_SUMMARY               	against aquaporin-4, the major brain water channel, expressed on astrocytes.
PR:PROJECT_SUMMARY               	Astrocytes in brain tissue from patients with NMO exhibit a spectrum of
PR:PROJECT_SUMMARY               	abnormalities and pathologies ranging from sublytic gliosis and reactivity to
PR:PROJECT_SUMMARY               	outright destruction. Our current working model for NMO pathogenesis involves an
PR:PROJECT_SUMMARY               	early and robust NMO IgG-induced astrocytic stress response that drives
PR:PROJECT_SUMMARY               	metabolic dyshomeostasis and the production of pro-inflammatory cytokines and
PR:PROJECT_SUMMARY               	chemokines that amplify pathology by recruiting innate immune effector cells to
PR:PROJECT_SUMMARY               	the CNS. Notably, astrocytes are implicated in clinical depression and patients
PR:PROJECT_SUMMARY               	with NMO experience depression at levels that exceed the general population.
PR:PROJECT_SUMMARY               	Preliminary magnetic resonance spectroscopy data from our group indicates that
PR:PROJECT_SUMMARY               	glutamate levels are reduced in the prefrontal cortex of NMO patients,
PR:PROJECT_SUMMARY               	suggesting that unipolar depression in these individuals is a direct pathogenic
PR:PROJECT_SUMMARY               	effect of NMO IgG-induced astrocytic dysregulation. Because astrocytes are
PR:PROJECT_SUMMARY               	critical for glutamine-glutamate cycling in the CNS, we hypothesize that
PR:PROJECT_SUMMARY               	stimulation of primary astrocytes with patient-derived NMO IgG will drive a
PR:PROJECT_SUMMARY               	metabolic shift marked by alterations in cellular levels of glutamate and
PR:PROJECT_SUMMARY               	glutamine. In preliminary experiments using 1H-NMR to measure metabolic changes
PR:PROJECT_SUMMARY               	induced in astrocytes by stimulation with NMO IgG we observed variable glutamate
PR:PROJECT_SUMMARY               	and glutamine responses. To overcome issues of signal-to-noise and the high
PR:PROJECT_SUMMARY               	basal levels of glutamate and glutamine produced by astrocytes, we now propose
PR:PROJECT_SUMMARY               	to use isotopic tracing and 13C-NMR to quantify NMO IgG-induced metabolic
PR:PROJECT_SUMMARY               	dysregulation. Our ultimate goal is to use NMO IgG-induced dyshomeostasis as a
PR:PROJECT_SUMMARY               	microscope to reveal basic mechanisms of pathogenic glutamate-glutamine
PR:PROJECT_SUMMARY               	metabolism in astrocytes that may not only impact the health of patients with
PR:PROJECT_SUMMARY               	NMO but may also yield novel insights into the mechanisms of depression in
PR:PROJECT_SUMMARY               	general.
PR:INSTITUTE                     	Mayo Clinic
PR:LAST_NAME                     	Howe
PR:FIRST_NAME                    	Charles
PR:ADDRESS                       	200 First St. SW, Rochester, Minnesota, 55905, USA
PR:EMAIL                         	howe@mayo.edu
PR:PHONE                         	507-284-9288
#STUDY
ST:STUDY_TITLE                   	TCA Isotopomers in Neuromyelitis Optica Patients (part - I)
ST:STUDY_SUMMARY                 	Patients with an inflammatory disease of the central nervous system known as
ST:STUDY_SUMMARY                 	neuromyelitis optica (NMO) experience increased levels of depression. These
ST:STUDY_SUMMARY                 	patients have an antibody that recognizes a type of cell in the brain called
ST:STUDY_SUMMARY                 	astrocytes – binding of this antibody to astrocytes triggers a stress response
ST:STUDY_SUMMARY                 	in the cell that results in the development of brain lesions that cause
ST:STUDY_SUMMARY                 	disability and cognitive disturbances. We recently observed a change in the
ST:STUDY_SUMMARY                 	level of glutamate in a part of the brain involved in depression in patients
ST:STUDY_SUMMARY                 	with NMO. Glutamate is a chemical that is used in the brain for communication
ST:STUDY_SUMMARY                 	between neurons – reduced levels of glutamate are thought to trigger
ST:STUDY_SUMMARY                 	depression by reducing neuronal activity in specific circuits. Based on this
ST:STUDY_SUMMARY                 	observation and the known role of astrocytes in maintaining glutamate levels in
ST:STUDY_SUMMARY                 	the brain, we hypothesize that the NMO antibody disturbs metabolic activity in
ST:STUDY_SUMMARY                 	astrocytes and thereby reduces glutamate and triggers depression. We intend to
ST:STUDY_SUMMARY                 	trace the metabolic response induced in astrocytes by the NMO antibody using TCA
ST:STUDY_SUMMARY                 	isotopomers. It is our hope that we will not only learn something about the
ST:STUDY_SUMMARY                 	mechanisms of astrocyte dysregulation in neuromyelitis optica, but that we will
ST:STUDY_SUMMARY                 	learn something about the mechanisms of depression in general that may lead to
ST:STUDY_SUMMARY                 	new therapies for this disease.
ST:INSTITUTE                     	Mayo Clinic
ST:LAST_NAME                     	Howe
ST:FIRST_NAME                    	Charles
ST:ADDRESS                       	200 First St. SW, Rochester, Minnesota, 55905, USA
ST:EMAIL                         	howe@mayo.edu
ST:PHONE                         	507-284-9288
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	MS6735-1	Time:23h + 1h nonCO2 | condition:BGPGt | matrix:pellet | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-2	Time:23h + 1h nonCO2 | condition:BGPGt | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-3	Time:23h + 1h nonCO2 | condition:BGPGt | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-4	Time:23h + 1h nonCO2 | condition:BGPGt | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-5	Time:23h + 1h nonCO2 | condition:BGPGt - 0.5mM Gn | matrix:pellet | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-6	Time:23h + 1h nonCO2 | condition:BGPGt - 0.5mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-7	Time:23h + 1h nonCO2 | condition:BGPGt - 0.5mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-8	Time:23h + 1h nonCO2 | condition:BGPGt - 0.5mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-9	Time:23h + 1h nonCO2 | condition:BGPGt - 5mM Gn | matrix:pellet | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-10	Time:23h + 1h nonCO2 | condition:BGPGt - 5mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-11	Time:23h + 1h nonCO2 | condition:BGPGt - 5mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-12	Time:23h + 1h nonCO2 | condition:BGPGt - 5mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-13	Time:1h nonCO2 | condition:BGPGt | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-14	Time:1h nonCO2 | condition:BGPGt | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-15	Time:1h nonCO2 | condition:BGPGt | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-16	Time:1h nonCO2 | condition:BGPGt | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-17	Time:1h nonCO2 | condition:BGPGt - 0.5mM Gn | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-18	Time:1h nonCO2 | condition:BGPGt - 0.5mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-19	Time:1h nonCO2 | condition:BGPGt - 0.5mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-20	Time:1h nonCO2 | condition:BGPGt - 0.5mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-21	Time:1h nonCO2 | condition:BGPGt - 5mM Gn | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-22	Time:1h nonCO2 | condition:BGPGt - 5mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-23	Time:1h nonCO2 | condition:BGPGt - 5mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-24	Time:1h nonCO2 | condition:BGPGt - 5mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-25	Time:0 hr | condition:E Med | matrix:Fresh med | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-26	Time:0 hr | condition:E Med | matrix:Fresh med | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-27	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-28	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-29	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-30	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-31	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-32	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-33	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-34	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-35	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-36	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-37	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-38	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-39	Time:0 hr | condition:BGPGt | matrix:Fresh med | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-40	Time:0 hr | condition:BGPGt - 0.5mM Gn | matrix:Fresh med | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	MS6735-41	Time:0 hr | condition:BGPGt - 0.5mM Gn | matrix:Fresh med | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-01	Time:23h + 1h nonCO2 | condition:BGPGt 0.5 mM Gn | matrix:pellet | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-02	Time:23h + 1h nonCO2 | condition:BGPGt 0.5 mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-03	Time:23h + 1h nonCO2 | condition:BGPGt 0.5 mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-04	Time:23h + 1h nonCO2 | condition:BGPGt 0.5 mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-05	Time:23h + 1h nonCO2 | condition:BGPGt 5 mM Gn | matrix:pellet | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-06	Time:23h + 1h nonCO2 | condition:BGPGt 5 mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-07	Time:23h + 1h nonCO2 | condition:BGPGt 5 mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-08	Time:23h + 1h nonCO2 | condition:BGPGt 5 mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-09	Time:23h + 1h nonCO2 | condition:BGPGt 50 mM Gn | matrix:pellet | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-10	Time:23h + 1h nonCO2 | condition:BGPGt 50 mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-11	Time:23h + 1h nonCO2 | condition:BGPGt 50 mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-12	Time:23h + 1h nonCO2 | condition:BGPGt 50 mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-13	Time:1h nonCO2 | condition:BGPGt 0.5 mM Gn | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-14	Time:1h nonCO2 | condition:BGPGt 0.5 mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-15	Time:1h nonCO2 | condition:BGPGt 0.5 mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-16	Time:1h nonCO2 | condition:BGPGt 0.5 mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-17	Time:1h nonCO2 | condition:BGPGt 5 mM Gn | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-18	Time:1h nonCO2 | condition:BGPGt 5 mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-19	Time:1h nonCO2 | condition:BGPGt 5 mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-20	Time:1h nonCO2 | condition:BGPGt 5 mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-21	Time:1h nonCO2 | condition:BGPGt 50 mM Gn | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-22	Time:1h nonCO2 | condition:BGPGt 50 mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-23	Time:1h nonCO2 | condition:BGPGt 50 mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-24	Time:1h nonCO2 | condition:BGPGt 50 mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-25	Time:0h | condition:E Med | matrix:Fresh med | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-26	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-27	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-28	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-29	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-30	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-31	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-32	Time:0h | condition:BGPGt 0.5 mM Gn | matrix:Fresh med | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-33	Time:0h | condition:BGPGt 5 mM Gn | matrix:Fresh med | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-34	Time:0h | condition:BGPGt 50 mM Gn | matrix:Fresh med | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6983-35	Time:0h | condition:E Med | matrix:Fresh med | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-1	Time:23h +1h nonCO2 | condition:BGPGt 0.5mM Gn | matrix:pellet | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-2	Time:23h +1h nonCO2 | condition:BGPGt 0.5mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-3	Time:23h +1h nonCO2 | condition:BGPGt 0.5mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-4	Time:23h +1h nonCO2 | condition:BGPGt 0.5mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-5	Time:23h +1h nonCO2 | condition:BGPGt 5mM Gn | matrix:pellet | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-6	Time:23h +1h nonCO2 | condition:BGPGt 5mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-7	Time:23h +1h nonCO2 | condition:BGPGt 5mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-8	Time:23h +1h nonCO2 | condition:BGPGt 5mM Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-9	Time:23h +1h nonCO2 | condition:BGP 50uMGt Gn | matrix:pellet | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-10	Time:23h +1h nonCO2 | condition:BGP50uMGt Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-11	Time:23h +1h nonCO2 | condition:BGP50uMGt Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-12	Time:23h +1h nonCO2 | condition:BGP50uMGt Gn | matrix:pellet | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-13	Time:1h nonCO2 | condition:BGPGt 0.5mM Gn | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-14	Time:1h nonCO2 | condition:BGPGt 0.5mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-15	Time:1h nonCO2 | condition:BGPGt 0.5mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-16	Time:1h nonCO2 | condition:BGPGt 0.5mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-17	Time:1h nonCO2 | condition:BGPGt 5mM Gn | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-18	Time:1h nonCO2 | condition:BGPGt 5mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-19	Time:1h nonCO2 | condition:BGPGt 5mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-20	Time:1h nonCO2 | condition:BGPGt 5mM Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-21	Time:1h nonCO2 | condition:BGP 50uMGt Gn | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-22	Time:1h nonCO2 | condition:BGP50uMGt Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-23	Time:1h nonCO2 | condition:BGP50uMGt Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-24	Time:1h nonCO2 | condition:BGP50uMGt Gn | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-25	Time:0h | condition:E Med | matrix:Fresh Media | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-26	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-27	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-28	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-29	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-30	Time:0h | condition:BGPGt 0.5mM Gn | matrix:Fresh Media | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-31	Time:0h | condition:BGPGt 5mM Gn | matrix:Fresh Media | Tracer:Unlabelled	
SUBJECT_SAMPLE_FACTORS           	-	ms6905-32	Time:0h | condition:BGP 50uMGt Gn | matrix:Fresh Media | Tracer:Unlabelled	
#COLLECTION
CO:COLLECTION_SUMMARY            	4,000,000 CLOD cells collected by methanol scraping. 1ml sup also collected.
CO:COLLECTION_SUMMARY            	There was no tracer element added to the sample. Also methanol extarction was
CO:COLLECTION_SUMMARY            	done in ice. After the extraction samples were kept in dry ice and delivered to
CO:COLLECTION_SUMMARY            	core.
CO:SAMPLE_TYPE                   	astrocytes
#TREATMENT
TR:TREATMENT_SUMMARY             	Murine derived pure cortical astrocytes were plated at 4x10^6 density in 10cm
TR:TREATMENT_SUMMARY             	petri dish and grown in astro growth medium (AGM) for 2-3 days until it reaches
TR:TREATMENT_SUMMARY             	the 90% confluency. Day before the treatment with glutamine or glutamate AGM was
TR:TREATMENT_SUMMARY             	changed to serum free “E” media. In case of isotomer analysis 40% of glucose
TR:TREATMENT_SUMMARY             	was switched with U13C6 (glucose) and added to serum free “E” media. On the
TR:TREATMENT_SUMMARY             	day of treatment first the spent media was collected and then washed with base
TR:TREATMENT_SUMMARY             	media. After washing, astrocytes were exposed to different ratio of glutamate:
TR:TREATMENT_SUMMARY             	glutamine for 1h under non-CO2 condition. 1h spent media was collected. After
TR:TREATMENT_SUMMARY             	washing three times with phosphate buffer saline cell pellet was collected in
TR:TREATMENT_SUMMARY             	ice cold 100% methanol by scrapping. All the samples were immediately
TR:TREATMENT_SUMMARY             	transferred to -70C for future analysis. E media composition: DMEM (w/o phenol
TR:TREATMENT_SUMMARY             	red)+ 1% PenStrep+ 10% FBS+ Glutamate+ Glucose Abbreviation: Gn-Glutamine
TR:TREATMENT_SUMMARY             	Gt-Glutamate B- Base media G- Glucose P- Sodium pyruvate
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	13C6 isotopomer analysis of Astrocyte We intend to test the experimental
SP:SAMPLEPREP_SUMMARY            	hypothesis that stimulation of primary murine astrocytes with patient-derived
SP:SAMPLEPREP_SUMMARY            	NMO IgG will drive a metabolic shift marked by alterations in cellular levels of
SP:SAMPLEPREP_SUMMARY            	glutamate and glutamine. To do so we will use isotopic tracing to measure
SP:SAMPLEPREP_SUMMARY            	glutamate and glutamine levels in cell extracts following stimulation with NMO
SP:SAMPLEPREP_SUMMARY            	IgG. The cell preparations, patient-derived antibody preps, isotopic tracer
SP:SAMPLEPREP_SUMMARY            	incubations, and cell stimulations will be performed in the PI’s lab.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	GC
CH:INSTRUMENT_NAME               	Agilent 7890B
CH:COLUMN_NAME                   	Agilent HP5-MS (30m × 0.25mm, 0.25 um)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:MS_COMMENTS                   	-
MS:INSTRUMENT_NAME               	Agilent 5977A
MS:INSTRUMENT_TYPE               	Single quadrupole
MS:MS_TYPE                       	EI
MS:ION_MODE                      	POSITIVE
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	Enrichment
MS_METABOLITE_DATA_START
Samples	MS6735-1	MS6735-2	MS6735-3	MS6735-4	MS6735-5	MS6735-6	MS6735-7	MS6735-8	MS6735-9	MS6735-10	MS6735-11	MS6735-12	MS6735-13	MS6735-14	MS6735-15	MS6735-16	MS6735-17	MS6735-18	MS6735-19	MS6735-20	MS6735-21	MS6735-22	MS6735-23	MS6735-24	MS6735-25	MS6735-26	MS6735-27	MS6735-28	MS6735-29	MS6735-30	MS6735-31	MS6735-32	MS6735-33	MS6735-34	MS6735-35	MS6735-36	MS6735-37	MS6735-38	MS6735-39	MS6735-40	MS6735-41	ms6983-01	ms6983-02	ms6983-03	ms6983-04	ms6983-05	ms6983-06	ms6983-07	ms6983-08	ms6983-09	ms6983-10	ms6983-11	ms6983-12	ms6983-14	ms6983-15	ms6983-17	ms6983-18	ms6983-20	ms6983-21	ms6983-22	ms6983-24	ms6983-26	ms6983-28	ms6983-29	ms6983-30	ms6983-31	ms6905-1	ms6905-2	ms6905-3	ms6905-4	ms6905-5	ms6905-6	ms6905-7	ms6905-8	ms6905-9	ms6905-10	ms6905-11	ms6905-12	ms6905-13	ms6905-14	ms6905-15	ms6905-16	ms6905-17	ms6905-18	ms6905-19	ms6905-20	ms6905-21	ms6905-22	ms6905-23	ms6905-24	ms6905-25	ms6905-26	ms6905-27	ms6905-28	ms6905-29	ms6905-30	ms6905-31	ms6905-32
Factors	Time:23h + 1h nonCO2 | condition:BGPGt | matrix:pellet | Tracer:Unlabelled	Time:23h + 1h nonCO2 | condition:BGPGt | matrix:pellet | Tracer:13C6	Time:23h + 1h nonCO2 | condition:BGPGt | matrix:pellet | Tracer:13C6	Time:23h + 1h nonCO2 | condition:BGPGt | matrix:pellet | Tracer:13C6	Time:23h + 1h nonCO2 | condition:BGPGt - 0.5mM Gn | matrix:pellet | Tracer:Unlabelled	Time:23h + 1h nonCO2 | condition:BGPGt - 0.5mM Gn | matrix:pellet | Tracer:13C6	Time:23h + 1h nonCO2 | condition:BGPGt - 0.5mM Gn | matrix:pellet | Tracer:13C6	Time:23h + 1h nonCO2 | condition:BGPGt - 0.5mM Gn | matrix:pellet | Tracer:13C6	Time:23h + 1h nonCO2 | condition:BGPGt - 5mM Gn | matrix:pellet | Tracer:Unlabelled	Time:23h + 1h nonCO2 | condition:BGPGt - 5mM Gn | matrix:pellet | Tracer:13C6	Time:23h + 1h nonCO2 | condition:BGPGt - 5mM Gn | matrix:pellet | Tracer:13C6	Time:23h + 1h nonCO2 | condition:BGPGt - 5mM Gn | matrix:pellet | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt | matrix:Spent Sup | Tracer:Unlabelled	Time:1h nonCO2 | condition:BGPGt | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt - 0.5mM Gn | matrix:Spent Sup | Tracer:Unlabelled	Time:1h nonCO2 | condition:BGPGt - 0.5mM Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt - 0.5mM Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt - 0.5mM Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt - 5mM Gn | matrix:Spent Sup | Tracer:Unlabelled	Time:1h nonCO2 | condition:BGPGt - 5mM Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt - 5mM Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt - 5mM Gn | matrix:Spent Sup | Tracer:13C6	Time:0 hr | condition:E Med | matrix:Fresh med | Tracer:Unlabelled	Time:0 hr | condition:E Med | matrix:Fresh med | Tracer:13C6	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	Time:0 hr | condition:BGPGt | matrix:Fresh med | Tracer:Unlabelled	Time:0 hr | condition:BGPGt - 0.5mM Gn | matrix:Fresh med | Tracer:Unlabelled	Time:0 hr | condition:BGPGt - 0.5mM Gn | matrix:Fresh med | Tracer:Unlabelled	Time:23h + 1h nonCO2 | condition:BGPGt 0.5 mM Gn | matrix:pellet | Tracer:Unlabelled	Time:23h + 1h nonCO2 | condition:BGPGt 0.5 mM Gn | matrix:pellet | Tracer:13C6	Time:23h + 1h nonCO2 | condition:BGPGt 0.5 mM Gn | matrix:pellet | Tracer:13C6	Time:23h + 1h nonCO2 | condition:BGPGt 0.5 mM Gn | matrix:pellet | Tracer:13C6	Time:23h + 1h nonCO2 | condition:BGPGt 5 mM Gn | matrix:pellet | Tracer:Unlabelled	Time:23h + 1h nonCO2 | condition:BGPGt 5 mM Gn | matrix:pellet | Tracer:13C6	Time:23h + 1h nonCO2 | condition:BGPGt 5 mM Gn | matrix:pellet | Tracer:13C6	Time:23h + 1h nonCO2 | condition:BGPGt 5 mM Gn | matrix:pellet | Tracer:13C6	Time:23h + 1h nonCO2 | condition:BGPGt 50 mM Gn | matrix:pellet | Tracer:Unlabelled	Time:23h + 1h nonCO2 | condition:BGPGt 50 mM Gn | matrix:pellet | Tracer:13C6	Time:23h + 1h nonCO2 | condition:BGPGt 50 mM Gn | matrix:pellet | Tracer:13C6	Time:23h + 1h nonCO2 | condition:BGPGt 50 mM Gn | matrix:pellet | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt 0.5 mM Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt 0.5 mM Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt 5 mM Gn | matrix:Spent Sup | Tracer:Unlabelled	Time:1h nonCO2 | condition:BGPGt 5 mM Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt 5 mM Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt 50 mM Gn | matrix:Spent Sup | Tracer:Unlabelled	Time:1h nonCO2 | condition:BGPGt 50 mM Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt 50 mM Gn | matrix:Spent Sup | Tracer:13C6	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	Time:23h +1h nonCO2 | condition:BGPGt 0.5mM Gn | matrix:pellet | Tracer:Unlabelled	Time:23h +1h nonCO2 | condition:BGPGt 0.5mM Gn | matrix:pellet | Tracer:13C6	Time:23h +1h nonCO2 | condition:BGPGt 0.5mM Gn | matrix:pellet | Tracer:13C6	Time:23h +1h nonCO2 | condition:BGPGt 0.5mM Gn | matrix:pellet | Tracer:13C6	Time:23h +1h nonCO2 | condition:BGPGt 5mM Gn | matrix:pellet | Tracer:Unlabelled	Time:23h +1h nonCO2 | condition:BGPGt 5mM Gn | matrix:pellet | Tracer:13C6	Time:23h +1h nonCO2 | condition:BGPGt 5mM Gn | matrix:pellet | Tracer:13C6	Time:23h +1h nonCO2 | condition:BGPGt 5mM Gn | matrix:pellet | Tracer:13C6	Time:23h +1h nonCO2 | condition:BGP 50uMGt Gn | matrix:pellet | Tracer:Unlabelled	Time:23h +1h nonCO2 | condition:BGP50uMGt Gn | matrix:pellet | Tracer:13C6	Time:23h +1h nonCO2 | condition:BGP50uMGt Gn | matrix:pellet | Tracer:13C6	Time:23h +1h nonCO2 | condition:BGP50uMGt Gn | matrix:pellet | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt 0.5mM Gn | matrix:Spent Sup | Tracer:Unlabelled	Time:1h nonCO2 | condition:BGPGt 0.5mM Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt 0.5mM Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt 0.5mM Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt 5mM Gn | matrix:Spent Sup | Tracer:Unlabelled	Time:1h nonCO2 | condition:BGPGt 5mM Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt 5mM Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGPGt 5mM Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGP 50uMGt Gn | matrix:Spent Sup | Tracer:Unlabelled	Time:1h nonCO2 | condition:BGP50uMGt Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGP50uMGt Gn | matrix:Spent Sup | Tracer:13C6	Time:1h nonCO2 | condition:BGP50uMGt Gn | matrix:Spent Sup | Tracer:13C6	Time:0h | condition:E Med | matrix:Fresh Media | Tracer:13C6	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:Unlabelled	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	Time:23h | condition:E Med | matrix:Spent Sup | Tracer:13C6	Time:0h | condition:BGPGt 0.5mM Gn | matrix:Fresh Media | Tracer:Unlabelled	Time:0h | condition:BGPGt 5mM Gn | matrix:Fresh Media | Tracer:Unlabelled	Time:0h | condition:BGP 50uMGt Gn | matrix:Fresh Media | Tracer:Unlabelled
CITRATE M0	99.91400428	84.97439018	85.63733836	86.19181978	99.84776373	89.60667712	89.5051327	90.4811567	99.79709077	91.78609541	91.39821321	91.0587441															98.99571783	98.56886833	99.02832756	99.11625542	98.87056113	98.75133581	67.22421684	67.09640651	64.85244764	67.66371415	67.20454394	65.85297687				100	89.7676625	89.38478631	89.00791122	100	92.6979456	91.5755554	92.61102422	100	90.10163363	90.79068583	90.40967747	80.21605992	81.0340729	100	84.97043432	84.12127856	100	84.15327308	85.78607821	98.14314952	98.01216855	67.37484775	67.51458616	68.33150653	98.44754159	86.70660186	87.22516458	87.31459877	98.0602919	87.69550684	86.25675913	85.93783696	98.16921969	88.02921206	86.96822115	88.38132439	100	79.50965758	91.06349612	82.44817569	99.78435366	67.27231511	66.44868662	67.58571351	100	85.54568385	82.49254354	90.21136981	100	97.39553598	63.33778127	63.26902722	63.72173482	100	100	100
CITRATE M+1		5.471047963	5.505446194	5.472924889		2.848775093	3.01603694	2.702418177		1.910055317	2.326768067	2.263570402															0.776658253	1.05575538	0.84950603	0.636138245	0.890641663	0.933767989	5.52111536	5.617335595	5.852714129	5.472409777	5.320141895	5.782062201					2.423579082	2.125497084	2.168151164		1.087055136	1.391055904	1.020499485		1.945868182	2.031581396	1.880765811	3.378012984	3.32186032		3.407524903	2.745197015		2.151154422	2.370086563	1.380154025	1.496278025	5.002385295	5.008918954	4.831890349	0.327442943	3.63718155	3.99957626	3.654113038	1.122836162	1.194438085	1.105511417	2.299138601	1.053017853	4.295715443	4.13169857	3.744202119			0.358907543	1.786153412	0.082428383	6.588154405	6.606122691	6.61060185		3.757214957	3.355214979	1.438927724		1.794616497	6.780120338	6.801112647	6.618848164			
CITRATE M+2	0.07871907	7.597122695	7.100143143	6.610323544	0.091167115	5.939142042	5.998658047	5.627554089	0.060389545	5.033173405	4.867400574	5.333923374															0.217077755	0.348989332	0.119797901	0.239332786	0.226413639	0.304148866	18.10436266	18.15528907	19.12454764	17.96277328	18.24535252	18.66694459					5.863242325	6.252432435	6.493066718		4.582599795	5.307780394	4.719280873		5.778927989	5.382943517	5.678118524	10.77995683	10.41468782		8.154567829	8.965470257		8.861922038	7.996010059	0.476696452	0.491553429	16.83680588	16.76320047	16.41869112	0.887073758	7.245754047	6.95785514	6.895772653	0.816871934	6.684315286	7.285444376	7.36629976	0.777762457	6.101003393	7.039891228	6.265994157		0.355977558	5.033995908	10.54312277	0.13321796	16.78195973	17.17829425	16.07147121		7.652819774	9.71698252	8.349702471		0.809847519	18.48679633	18.54750041	18.41668528			
CITRATE M+3		1.453244329	1.301034233	1.300802188	0.037212184	1.258636128	1.122755565	0.878306917	0.011833578	0.901901569	0.932831097	0.816270728																					6.26447405	6.217857752	6.865463368	6.112342861	6.37682277	6.656617669					1.945516092	2.237284171	2.3308709		1.632399469	1.725608306	1.64919542		2.173570201	1.794789257	2.0314382	4.998776349	4.702025878		3.439824589	3.862402529		4.15916634	3.49741363			8.859912933	8.797836617	8.604839046	0.337941705	2.410462544	1.817404016	2.13551554		3.860980134	4.48785993	4.017014842		1.574069105	1.860189057	1.608479332		3.408179101	3.543600425	5.222548127		8.163106194	8.394551213	8.329028638		3.044281419	4.435258965				9.553020148	9.550742253	9.450617955			
CITRATE M+4	0.001581187	0.388752111	0.325537118	0.317355063		0.225478543	0.247691816	0.207323916	0.095598429	0.308730287	0.337772395	0.370332209																					1.787225269	1.806749499	2.064965267	1.73661863	1.753578804	1.902639877																0.627193921	0.52735309		0.027648361	0.305651642		0.674484119	0.350411541			1.926048141	1.915457795	1.813072959						0.56475966	0.864425146	0.379709836						6.606733394				1.194464558	1.37234522	1.403184792							1.842281911	1.831617469	1.792113782			
CITRATE M+5	0.005174236	0.111756499	0.126922194	0.102030924	0.023856968	0.121291077	0.109358995	0.103240199		0.043625223	0.079688273	0.08062517															0.010546161	0.026386959	0.001271546	0.007528443	0.010708069	0.010747336	1.082813223	1.095253424	1.230240554	1.035434025	1.092370367	1.12374334																																										10.11945237																		
CITRATE M+6		0.001295471		0.004743608					0.035087681	0.005464879	0.050707934	0.066238488																	0.000835938				0.015792607	0.011108146	0.009621405	0.016707278	0.007189704	0.015015459																																																												
α-KETOGLUTARATE M0	99.9050714	85.93261657	87.03761372	87.13331473	99.75257437	92.73219091	92.58031428	93.2229047	99.79788578	94.95270175	94.90219575	94.8116527	94.63899098	82.058732	80.93362138	82.17334727	96.31171087	88.34951198	86.84285168	88.78210868	97.49734021	90.94622934	89.58511701	92.31659541			99.84116631	99.88115171	99.86493568	99.77320925	99.72347581	99.84537536	64.87672066	65.12672844	62.68311991	65.28223122	64.66582811	64.45479271				99.52231925	93.92151965	93.99179118	94.00230084	98.56539417	97.01969405	96.61321896	97.00004205	98.60824932	94.29714044	94.0467426	94.36514038	97.71357427	100	100	100	100	100	100	99.91346711	98.52498606	98.93741867	87.73611687	87.13779793	87.53347224	100	89.17130575	90.2949232	88.74353659	99.17444008	93.33296295	92.92362024	93.64156189	98.6937861	91.12882912	91.17559938	91.68449178	100	87.80282653	88.85932269	95.01853658	100	88.95045174	86.8845126	84.96773156	99.90764733	94.2361265	88.92728751	89.46275957	85.30935313	99.57457091	60.08580253	60.91400582	60.83450469			
α-KETOGLUTARATE M+1		5.742423568	5.353681739	5.332992262		2.231075718	2.325789221	2.042370816		1.206901823	1.45174848	1.420607801		5.467993775	5.990017822	4.830438436		2.278407878	3.09633267	2.131568672		1.319666527	2.227564527	0.969800588									7.523094009	7.542197149	8.090261563	7.568044698	7.686103508	7.826807225				0.477680751	3.038956108	2.646486577	2.853346259	1.43460583	2.360254096	2.143606043	2.623752486	1.391750683	3.594429849	4.1615884	4.283536238	2.286425726							0.086532891	1.475013937	1.062581328	12.26388313	12.86220207	12.46652776		4.337154015	3.552878371	7.839339102	0.570768814	2.203613072	2.15876647	1.666377581	0.942684627	3.481660667	3.105342833	3.31996016		7.036361104	3.984015457	2.496946423		1.962509754	4.909410007	4.344258484	0.092352675	1.952244053	5.738611491	3.694324774	14.69064687		8.521196522	9.009698001	8.373945473			
α-KETOGLUTARATE M+2	0.028202173	6.521477793	5.973909142	5.802473875		3.891709377	4.054851261	3.630031609		2.910964169	2.846922354	2.935342479	5.320539313	9.884950609	11.10440436	10.84817822	3.670204741	8.229703008	8.408142532	7.623015268	2.490017009	6.955517879	7.189094862	5.797150221			0.063413735		0.050381512	0.120964866	0.172603563	0.051177215	20.25601352	20.08747146	21.14841131	20.02696617	20.27019211	20.35599839					2.121286078	2.371863381	2.122442915		0.214809051	0.692337308	0.078607639		1.258234373	1.104400474	0.664653745															5.625562391	5.391268117	3.417124308	0.254791107	3.784936857	4.012520806	3.804825796	0.363529275	4.641362745	4.700004989	4.433044233		5.160812369	7.156661853			6.7487451	6.628436723	7.949745301		2.276844119	2.144969242	6.720338525		0.008360708	22.19238589	20.57088315	21.58307812			
α-KETOGLUTARATE M+3	0.015838471	0.983561288	1.004572093	1.012775968	0.020090519	0.570599796	0.534820396	0.524976248	0.039245944	0.450383709	0.449408676	0.422107251	0.012171434	2.007747319	1.010800887	1.578526465		0.860881281	0.991289642	0.770723879		0.24673894	0.559101613	0.726682806			0.062804121	0.086743045	0.071594008	0.092486514	0.073649847	0.081520876	4.784084339	4.700843579	5.164641945	4.637786667	4.808559205	4.779676824					0.918238164	0.989858858	1.021909987		0.405242808	0.550837691	0.297597821		0.850195339	0.687268523	0.686669638															0.865977843	0.760930316			0.678487122	0.905092483	0.887234734		0.748147468	1.0190528	0.562503825				2.484516999		1.806730086	1.577640675	2.738264656		1.534785325	3.189131752	0.122577135		0.129726748	7.52305018	7.768598257	7.518760434			
α-KETOGLUTARATE M+4	0.00464934	0.285588539	0.267920071	0.254886364	0.022823572	0.173116449	0.172206782	0.167462349	0.01652339	0.139157417	0.13716049	0.109698211		0.244312295	0.492516922	0.224077549		0.267700307	0.36017201	0.457716775		0.423887295	0.140636704	0.180924674			0.021386301	0.024490855	0.001073417			0.019431996	1.875086795	1.860677446	2.150976909	1.815933342	1.864032454	1.871283298																																														0.531563318								0.089359938	1.073918132	1.251618208	1.248589344			
α-KETOGLUTARATE M+5	0.00689289	0.104877506	0.101296218	0.150876385	0.002830095	0.101584548	0.062333003	0.056091296		0.053649442	0.046751543	0.097731629		0.114896346	0.191600361	0.090469734			0.029489218	0.068041682		0.100561389	0.112619811				0.00235125				0.00960378		0.669221059	0.672024644	0.759229615	0.659607391	0.691449658	0.706305154																																																						0.197981701						
GLUTAMATE M0	96.26538095	84.78093826	84.93427958	85.84672868	95.40490809	90.20024577	89.79807295	90.06224704	99.65819308	95.96769752	96.08438067	96.5380654									99.73470128	97.4910744	98.38275421	98.47167003	95.1361384	98.42558108													97.52972169	98.4566134	99.56852837	98.48226184	90.55216236	89.91898323	89.72723234	95.22242796	93.0083773	93.70972648	93.53612348	97.94994557	90.56362735	92.15995988	90.36004587	100	100	100	100	99.56052872	100	100	98.63908471	100	100	100	100	100	99.24481125	92.3791629	93.1037212	92.79371425	99.91749956	96.94679664	96.54534634	96.77584408	99.74313406	94.78964779	94.16684157	96.56696853	100	100	100	96.98647557	100	100	100	100	100	100	100	100	100	100	100	100	100	100	99.75148893	98.86098569
GLUTAMATE M+1	2.835153991	7.121648276	7.390477601	7.070071802	3.49833825	4.999508419	5.171440935	5.120965454	0.28433738	1.606081682	1.619084603	1.040645406										0.754056692	0.816749288	0.230599381																		1.114148465	4.406310476	4.588387234	4.360511246	3.497269574	3.873944524	3.111271489	3.473302648	1.568062983	4.253366355	3.502316578	5.098196698					0.439471283			1.360915293							3.161556071	2.995784687	2.669103847		1.1191482	1.143649101	0.854433955		2.462457058	2.355475238	1.798734351				3.013524432																1.054271158
GLUTAMATE M+2	0.883257112	6.868361693	6.544747532	5.995057537	1.082792069	4.409230527	4.64504208	4.522066009		1.964766225	1.899078784	2.070220281										1.103677951		1.208104191	4.657855682	0.842948038													2.425578591	1.421374257	0.368186885	0.403589696	4.644435329	5.053024671	5.256286864	1.280302467	3.117678172	3.134161373	2.99057387	0.481991445	4.756434009	3.975312388	4.541757434															4.111912111	3.160641369	4.189359446		1.218456202	1.400546232	1.437542963		2.42882036	2.782675388	1.226555804																				0.084743152
GLUTAMATE M+3		0.95158293	0.884680537	0.84824174		0.288924941	0.283873302	0.208412211		0.394996526	0.2908049	0.302518092									0.265298718	0.550815022	0.800496503		0.156592273	0.727327287														0.122012345	0.053711032		0.397091838	0.439604864	0.655969547			0.044840661			0.426572289	0.362411153															0.424861482	0.347368916	0.739852746	0.347822461		0.68627705	0.832815689	0.789772866	0.128621224	0.319074791	0.695007805	0.407741314																			0.24851107	
GLUTAMATE M+4		0.204281304	0.186035417	0.168234384		0.062262738	0.057346657	0.050617426	0.05417154	0.052767463	0.071311835	0.037437377												0.062289523																																											0.330327271				0.082500436	0.029321908	0.077642635	0.142406137	0.095832591																							
GLUTAMATE M+5	0.016207942	0.073187537	0.059779334	0.071665855	0.013961595	0.039827607	0.044224075	0.035691864	0.003298003	0.013690587	0.035339204	0.011113441										0.100375939		0.027336871	0.049413643	0.004143597													0.044699715		0.00957371																																		0.032412125																							
GLUTAMINE M0	99.50807562	82.10215109	83.11559607	82.58853865	95.82093371	93.4580178	86.79849569	93.08269326	99.97477757	99.78602391	99.53776594	99.6334748	99.74798008	73.52400517	74.07070986	72.11892121	94.52078382	94.25147563	94.25410792	92.30926139	99.73612136	99.76981877	99.70135039	99.8217259			99.91141922	99.94463982	99.8811023	99.93183823	99.830187	99.69074701	70.19352186	70.30070597	67.56581155	70.45646841	69.89057496	69.03294		99.59754157	99.99227323	99.7675164	92.56826228	93.945162	93.58826579	95.5618639	94.4630164	94.10722911	95.18332095	98.51640021	94.72720154	94.24896978	93.28931702	95.38474107	95.70368336	100	98.47601325	93.77670483	97.05395563	96.65569966	95.61075232	99.4907282	96.22713403	68.8035361	68.80760482	69.60613431	99.98607156	100	96.4860614	96.01792216	97.49615092	95.16866281	94.54121535	95.89465542	100	95.81539434	95.74788228	96.21526955	99.26192722	100	97.691788	97.57282484		95.71871397	95.62538195	94.40926165	98.04309494	98.72895295	100	100	100	99.80715545	63.96326048	62.93472425	63.27323662	99.98640977		99.80839773
GLUTAMINE M+1	0.334949488	5.307490611	5.875794688	5.702331817	3.305198464	4.224477717	9.604451481	4.497466697		0.032011159	0.212422798	0.113710067		7.101448801	6.636321829	6.747247144	4.304632546	4.10991505	3.960482328	5.449286791	0.214444483	0.146079782	0.08824959	0.053869782					0.104265284		0.14977108	0.231598669	6.611175176	6.439542545	7.083531856	6.485356896	6.624125993	6.920382819		0.390868813			2.378823422	1.715926044	1.860919166	3.27225157	3.711242302	4.022681688	3.054381058	1.183137536	1.236581628	1.829973889	2.528608299	2.640050676	2.610028315			4.351879514	2.193831243	2.077159266	2.72140179		2.612893045	6.968954256	7.328203505	6.707821326			0.891124676	1.49659478	2.018877579	3.335820579	3.373991697	2.819956879		1.788157839	1.773052221	2.086139672	0.738072782		1.014229462	1.476939165		3.130906123	3.165088028	4.382016	1.95690506					0.192844552	9.179284211	9.156167102	7.926427761			0.191602266
GLUTAMINE M+2	0.067471488	8.841710117	8.476034849	8.298380573	0.837839384	2.220805397	3.471250414	2.339535688		0.063192836	0.154445029	0.122000118		14.29824546	13.98066634	15.19737485	1.143112981	1.580525228	1.717632305	2.185794452													17.13956834	17.21144233	18.39821133	17.06058782	17.34006958	17.69116355					4.007036529	3.390605429	3.556437405	1.165884531	1.825741297	1.870089199	1.762297993	0.300462255	3.067965295	3.12223026	3.480595838	1.975208256	1.686288322	3.474282606	1.220100039	1.871415651	0.752213124	1.267141072	1.667845892		1.159972928	17.04987314	16.94935846	16.72080403			2.035808376	2.148659275	0.484971501	1.495516612	2.084792952	1.285387705		2.300092084	2.241001729	1.517939439			1.214239161	0.950235998		1.150379909	1.209530025	1.208722352		0.400076409					20.36733778	21.32733604	21.34790219			
GLUTAMINE M+3	0.033311514	2.218649855	1.710324116	1.805354059					0.002113944	0.073925969	0.069926222	0.11798683	0.128529736	3.279799225	3.418440944	3.749458404					0.029658686	0.074382523	0.178569229	0.103000964			0.050200598	0.031384267	0.007175201	0.026907217	0.01145637	0.065750615	3.907637991	3.907162988	4.511664906	3.937309473	4.009973872	4.107686192			0.000137186		1.045877767	0.948306523	0.994377641						0.968251533	0.798826076	0.701478845				0.303886708					0.453374213		6.502862703	6.409773357	6.236168265			0.587005545	0.336823783						0.096355742	0.238063769	0.180651341			0.079743375							0.515979234					6.490117529	6.581772609	7.280975494			
GLUTAMINE M+4		0.647237074	0.570075408	0.74894642		0.04409317	0.039630616	0.030462842	0.020743551	0.016675512	0.020694036	0.012606025	0.007991575	1.218642653	1.296120628	1.457201244		0.017603215	0.024519581		0.017887445	0.008540156	0.030063832	0.021403349			0.029840703	0.01639567			0.002827		1.588284545	1.570833319	1.787132833	1.492656147	1.561024101	1.665631584		0.007114335																								0.674773802	0.505059859	0.729072069	0.013928436																					0.285262108							0.171457934	0.013590233		
GLUTAMINE M+5		0.25249334	0.187494074	0.293688756	0.020886109	0.04475502	0.082666498	0.045286663		0.024175308	0.004239517			0.447641052	0.450652678	0.510677867	0.02687985	0.039988088	0.042420242	0.053413917							0.008539484	0.007580247	0.00623917	0.041254552	0.005758548	0.009514474	0.559678427	0.561873177	0.65364753	0.563169887	0.564797249	0.577561497		0.002529705	0.006083326																					0.055897583																										0.069729303										
SUCCINATE M0	95.70947208	86.80295241	88.85634436	85.59995691	87.30053578	80.6784103	84.71839512	84.85041265	73.35848448	51.71827594	48.12505025	64.73161708																															99.96535165	99.65925544	99.53552479	100	94.84073683				100												99.90416073	86.61034193	81.82163467		99.96231052	94.43939447	96.28067773	94.58181962	100	97.94377051	98.84362235	96.96547822	100	94.68780943	96.5637411	96.92650721	100	100	100	100	100	100	100	100	100	100	100	100	100	100	97.08792939	94.72111821	95.04958728	100	100	100
SUCCINATE M+1	3.953785414	9.35918951	8.41568319	11.00050092	12.37682085	17.89136088	13.85358473	13.69643903	26.38244854	47.51262457	51.11602081	34.09419632																																	0.223715693		5.159263172																					1.447888911	0.462640682	1.777824861						2.16400292	0.20409773	0.90367356																				
SUCCINATE M+2		3.015666804	1.827480134	2.50390056		0.809607904	0.696127916	0.755780598		0.388915582	0.200783286	0.599314418																																																				8.87256299	13.1031051			3.526201605	2.464324532	3.36736296		1.580201976	0.788444912	2.43670492		2.77234711	2.466669149	1.790948569																				
SUCCINATE M+3	0.01927562	0.149951706	0.236440098	0.033670498		0.088779518				0.072956516																																		0.322128559	0.240759518																			2.789390687	3.536982681			0.586515011	0.792357055	0.272992556		0.43607671	0.191561588	0.556102991		0.375840535	0.687908664	0.378870661																				
SUCCINATE M+4	0.317466883	0.672239573	0.664052215	0.861971109	0.322643362	0.531841399	0.731892233	0.69736772	0.259066986	0.307227397	0.558145657	0.574872179																															0.03464835	0.018616																			0.095839274	1.727704394	1.538277549		0.037689477					0.039950804	0.176371153	0.041713867			0.077583357																2.912070614	4.272890459	4.051342838			
FUMARATE M0	99.2033576	89.97978608	89.46275472	89.72447876	99.42919794	94.08990217	92.7916597	93.58169934	99.65168138	94.95283229	94.26099419	94.09450029	98.11967842	90.24566545	85.6149735	86.03725416	86.47008329	84.31130061	83.05172761	78.29650136	82.53006914	79.6987784	71.74174966	79.14079498			98.00367986	99.37683086	98.68264278	97.54974927	99.22687516	99.62311657	72.78374838	72.6517195	70.90127576	69.58686509	71.45577185	71.31886752				99.30852346	86.60302332	86.17349702	89.20930429	97.96297126	89.87660569	92.36022611	89.49428047	100	89.18963962	90.51240944	93.23020022	100	100	100	100	100	100	100	100	99.37111263	100	100	100	100	99.8786833	96.59251451	97.21877579	94.68148984	100	96.434438	96.1556978	96.0458124	100	96.34566837	97.62080347	96.94145401	100	100	100	100	100	100	100	100	100	100	100	100	90.56168567	100	100	95.32588246	100	100	100	100
FUMARATE M+1		3.921424154	3.67070127	3.347369201		1.507155745	1.606182689	1.299082616		0.687083376	0.773787778	0.78199019	1.606062702	6.483836068	11.80001312	10.93598891	13.10241677	13.00143239	14.30413751	17.23127015	16.03307739	19.64088983	26.48655727	18.40297202			1.720517599	0.353691883	1.00010898	1.982228743	0.434206383	0.124725473	5.983675479	6.133725399	6.313800304	5.602339108	5.667816296	5.34537221					5.396184934	1.314078996	1.055249405		1.563998057	0.759930904	7.712109523		0.72018498	5.866083175	1.674273618									0.628887373						2.22883491	0.380729078	2.443976085		0.93084644	0.834886092	1.609374509		1.679661446	0.612829097	1.460021621													9.438314331			4.674117545				
FUMARATE M+2		4.089583057	4.228106637	4.243487629		2.730614885	3.064875044	3.17692847		2.746258567	2.747268478	2.856072663		2.999824361	2.225560584	2.689138691		2.313516185	2.251428043	4.08008403	0.993206223	0.149455765	1.191200303	2.002712858					0.056091411	0.196683186	0.08606199		8.05396411	8.178877366	8.830497822	8.194945781	8.464418877	8.687433046				0.691476542	5.725313516	8.059676749	8.730331491	2.037028741	7.646607899	3.904560508	1.189578816		8.742051466	3.621507387	4.274426086															0.170402868	0.298491592	1.034677346		0.87967704	0.918816679	0.477460911		1.07040116	0.601308553	0.947088434																				
FUMARATE M+3	0.796642401	1.966045827	2.638437375	2.62653536	0.570802062	1.624786398	2.537282564	1.895482291	0.348318617	1.589801989	2.217949558	2.26743686																					11.16209878	11.93035978	12.48708423	16.37323972	13.96577182	14.64832723					2.275478226	4.452747234	1.005114809		0.912788357	2.975282482	1.604031195		1.348123932		0.821100073															1.00824771	2.093564886	1.839856726		1.755038524	2.090599427	1.867352181		0.904269023	1.165058878	0.651435932																				
FUMARATE M+4		0.04316088		0.058129055		0.047540802		0.046807287		0.024023781			0.15547274	0.067032038	0.147186716	0.120109468	0.254948098	0.154363089	0.168694745	0.119021917	0.236629586	0.304267563	0.322670544	0.216007711			0.15650044	0.169311927	0.140034658	0.143043559	0.13368414	0.137775843	2.016513253	1.105317953	1.129010691		0.446221163																																																													
MALATE M0	99.89064626	90.13423149	91.43395771	92.58427515	99.93241839	95.45873178	95.09649903	96.54689833	99.29435746	98.26573565	97.65862185	97.64880585	92.31447223	90.8797213	89.82868358	89.72338298	97.14015249	93.58926882	92.00058154	94.62585985	99.5394736	96.76549657	97.3363267	98.27593196	99.0196008	98.80868547	99.11828529	99.20546458	99.35107279	99.21010086	99.1343093	99.12150286	76.82860738	74.52531765	73.6414718	75.05766179	74.38238587	74.32095933				99.99897863	94.63798688	94.76970896	94.21905594	100	97.26065134	96.76379892	96.87127659	100	94.42618577	94.57479072	94.32021227	98.29162124	100	100	92.39817079	94.10740667	97.11697046	100	90.18346904	98.34822903	100	82.02079326	80.17878948	82.28763524	99.31751274	92.34607624	93.85711594	93.49979523	99.35257317	92.16740634	91.69006387	91.79978508	100	93.42566162	94.92412328	93.83945739	99.40244825	84.53025047	81.61345825	81.67044437	100	85.46508125	83.42547914	88.18534858	98.34770124	77.44387976	93.95160264	98.6032476	100	99.64178841	69.66399577	70.01568099	69.50365313		100	
MALATE M+1		4.892058359	4.454478496	3.711332055		1.655093218	1.860107711	0.916525296		0.009225608	0.05476582			0.710539122																			4.799299807	5.654993135	5.726746127	5.435370648	5.668437756	5.85456788					2.095528733	1.525243647	1.899764503		0.902523404	0.743050821	0.946886037		2.007357955	2.225892932	2.288101264	1.708378759			3.6541446	1.260286059	2.883029543		5.129769227			0.14263455			0.635766071	3.82196452	3.1931747	3.26260571	0.647426826	2.977659226	2.829858616	3.181497526		3.493101178	2.325127584	3.401385652		11.15415273	13.52910668	11.56375699			1.440520958		1.652298764	17.15565552					8.936888592	6.473140163	8.173824488			
MALATE M+2		4.034214891	3.194036125	2.7479588		2.195859467	2.320728489	1.848522321		0.988867505	0.962695377	1.061079571	7.269871051	8.409739573	10.17131642	10.27661702	2.859847513	6.015195616	6.825357812	4.415378963		1.497490064	0.508805849	0.287513799									9.416200951	10.54559954	10.68515127	10.27754276	10.58880527	10.49749302					2.548597204	2.631964187	2.819713338		1.402328712	1.683177051	1.587157308		2.607241881	2.492896347	2.643110217				3.947684614	4.632307273			4.686761728	0.853109311		7.459249851	8.854633457	6.57470143	0.046721184	3.25868035	2.575787547	2.682689735		3.046649353	3.198922502	3.159449668		2.720548809	2.049605077	2.536726603		3.199994231	1.519457164	1.780583539		3.711974588	4.324695623	2.25576611		3.689139799				0.358211586	11.72120797	13.14989395	12.51333876			
MALATE M+3	0.075004087	0.785765774	0.736827648	0.764545068	0.033291913	0.575444185	0.604204736	0.584094236	0.051749479	0.477789901	0.472620565	0.447759067									0.164264403	1.087155719	1.139620943	0.848044318	0.420669328	0.233778197	0.031636135	0.170858066	0.096299211	0.255654721	0.187668135	0.143324862	7.964873817	8.276888689	8.886267732	8.199339646	8.430238958	8.372355892					0.717887186	1.073083206	1.061466218		0.434496539	0.795555807	0.594680062		0.959214395	0.706419996	0.748576248									0.79866166		7.626056028	8.097478808	8.17255801		0.57327889	0.373921809	0.55490933		1.808285084	2.281155013	1.85926773		0.360688389	0.701144057	0.222430352		1.115602567	3.337977909	4.985215102		10.82294416	10.80930427	9.558885306		1.711324929	6.04839736	1.396752401			9.113728948	9.199619408	9.315601441			
MALATE M+4	0.026611048	0.150877084	0.180700016	0.190886336	0.003882332	0.077073247	0.100066532	0.061779269	0.025223825	0.073727472	0.061700635	0.045407683							0.164754918	0.008311736					0.559729876	0.957536331	0.558802343	0.311445488	0.280229354	0.368733809	0.379980333	0.430484905	0.852026432	0.837925687	0.996942764	0.909838852	0.723443932	0.780110286				0.001021371						0.014417399																2.453177988	2.869098258	2.965105322																											0.564178729	1.161665489	0.493582181			
ASPARTATE M0	99.97640454	89.05281963	89.08511641	89.73418714	99.62874756	93.56627945	93.06617397	93.10878242	96.72057323	93.74550607	93.75881834	93.28347028																														98.71453274	92.74579306	93.78755414	92.91181704	96.46803902	94.21022553	94.02704257	93.87153301	98.18054939	92.85693092	92.43881542	92.6955277	100	100	100	100	92.83208304	99.60155721	91.98782654	90.64678416	100	100	100	100	100	100	92.7203331	93.5203542	93.67195418	99.98789756	94.40383754	93.13520346	94.16477653	100	94.42690956	93.9466221	95.13991297	100	92.90900278	91.68407032	98.56239329	91.26527614	84.28061634	79.39363224	85.54609123	100	89.46805593	96.55834293	80.57699097	92.66681048	99.55586732	79.61007139	93.72628479	90.44475511			99.8382187
ASPARTATE M+1		5.631536228	5.884861832	5.605493838	0.209079152	3.023192258	3.341897835	3.494406377	2.480252217	3.641569131	3.716973462	3.867392083																														0.982402848	3.487247383	2.309538428	2.941425579	2.712393097	3.254259661	3.046215916	3.389866435	1.403284161	3.394949839	4.191284794	3.90022763					7.167916962	0.398442791	8.012173459	9.353215835							2.983274851	2.673471392	2.568507703		0.649784721	0.81807559	0.78188903		2.261640623	2.437423689	2.001647843		7.090997216	8.315929676	1.437606709	8.73472386	9.045289833	10.12480886	13.76562711		9.670530846		19.42300903	3.672586432	0.444132681	20.38992861	6.273715214	7.605975867			0.161781297
ASPARTATE M+2		4.42527576	4.237835939	3.900548988	0.157807505	2.957823459	3.111756816	3.036596783	0.788433126	2.468080144	2.405219204	2.746749147																														0.303064408	3.117522313	2.79646226	3.088619561	0.819567888	2.331097466	2.500593173	2.507214074	0.416166453	2.991164634	3.00563767	2.931450873															3.180206377	2.951368263	2.874079151		2.401049338	2.617074911	2.46355271		2.591825868	2.804268012	2.30112267																	1.949269023			
ASPARTATE M+3		0.754039961	0.683437099	0.656795348		0.418280443	0.438795055	0.330228427		0.124586018	0.105043404	0.090907025																															0.649437248	1.106445177	1.058137817		0.20441734	0.426148336	0.231386479		0.756954604	0.364262113	0.472793791															1.116185673	0.854806146	0.885458968		2.169547067	2.888527072	2.220255671		0.719623951	0.811686202	0.557316514						6.674093829	8.839817368			0.861413224	3.44165707		3.660603085							
ASPARTATE M+4	0.013208924	0.136328418	0.108748715	0.102974683	0.004365783	0.034424386	0.041376323	0.029339527		0.010315885	0.008977463																																																												0.01210244	0.375781333	0.541118971	0.369526061											1.641741539	0.688281665												
LACTATE M0	99.90585807	90.17850801	91.16196462	92.95423267	98.82581814	89.9984414	91.44089074	92.03163241	99.79393524	94.23770592	94.23591146	93.33433492	99.45516446	88.63227624	89.97887697	91.06747133	98.91747288	88.61786257	89.27718602	90.38735169	98.90726752	91.91102527	91.02863837	90.70379419	99.78563399	99.87827881	98.72379995	99.59169202	99.98444121	99.81654435	99.98895896	99.53539168	68.12683148	67.35846575	66.57620217	67.71772997	68.14967712	67.24601247	99.61271866	99.72312746	99.71940014	96.82177319	96.27349774	96.34686411	95.39088693	98.82915742	96.82983504	97.9344659	97.52325191	98.824604	95.04047115	96.91261116	94.96399007	87.13370012	88.44596067	99.94027661	90.06297344	91.25280497	99.77130511	89.69885108	91.11084764	97.48626868	97.35042082	63.90365537	64.08573008	65.0586596	99.24069406	94.00738661	96.95019315	95.38722228	99.37515004	82.89168335	81.55223463	83.92166917	99.1751552	96.34188735	97.30221748	94.98014195	99.93225434	88.36783843	91.32775535	89.9987047	99.95955114	62.55353418	62.88512938	64.22377697	99.92505191	89.90042781	88.05340847	90.42384443	98.38054729	99.96572521	63.05797738	63.01795979	63.50426919	96.0086575	93.24611633	97.52220953
LACTATE M+1	0.028951121	1.433282735	1.179113506	0.711631445	0.895952333	1.508321051	1.081458439	1.016783884	0.107056096	0.530180264	0.661491537	0.830174135	0.425182186	1.750240324	1.508088094	1.482751187	0.848619503	1.539388399	1.413574694	1.721703289	0.845041098	1.552501373	1.270730242	1.3886936			1.005329527	0.349782578		0.146507266		0.36761943	2.118846757	2.471742863	2.278688614	2.47507646	2.15909859	2.455504791																								2.006668934	2.103553119	2.9804431	2.890942769	2.494453578						1.181678222	0.46041322	0.905275222						0.237370553				10.13437483	9.23800886	8.428931562							0.841186174	0.94227528	0.727271689			
LACTATE M+2	0.061032162	1.175303265	1.026107567	0.823782136	0.278229528	1.16275453	0.992613019	0.921250529	0.099008661	0.708611181	0.735472327	0.775466232	0.119653356	1.297734704	1.157436257	1.052677946	0.232683576	1.259280884	1.196036176	1.168399363	0.245211733	1.043516406	1.02948605	1.055591796			0.267514141	0.058081214		0.036948385		0.096988895	2.947227196	3.019890653	3.056389365	3.009179201	2.899319622	3.0532308																0.774275719	0.683644345		0.56613534			0.241064027	0.262706281	0.507062384	0.546026062	3.223835026	3.180431346	2.960516716		0.233899497		0.092264552		1.469219415	1.598909181	1.485991968			0.304638978			1.116686381	0.783130547	0.980916451		4.449335075	4.307022469	4.036100533		0.859450542	1.070116541	0.751725636			3.005225661	3.038960871	2.984108652			
LACTATE M+3	0.004158652	7.212905989	6.632814304	5.510353753		7.330483015	6.485037805	6.030333173		4.523502633	4.367124672	5.060024711		8.319748736	7.355598676	6.397099538		8.583468152	8.113203114	6.722545656		5.49295695	6.671145339	6.851920417	0.199899707	0.119375733			0.011575806		0.011041035		26.80709456	27.14990074	28.08871985	26.79801437	26.79190467	27.24525194	0.383123775	0.276872537	0.280599858	2.582592137	2.981401263	2.811139456	3.70604861	0.677271315	2.460516024	1.491578863	2.476748092	0.460768303	3.862880667	2.315870748	3.844471077	10.32368625	9.262651505	0.059723389	8.161057164	7.432093284	0.198713131	8.566591237	7.334984514			25.83226577	25.81168513	25.47839377		3.81939176	1.816970546	3.095397954		12.31889084	13.76509988	11.59076698		2.319561294	1.723257848	2.734789735		8.773246431	6.684441088	7.669272936		21.29903607	21.72154697	21.33176445		7.810734694	9.266248562	7.453184635	0.516798369		28.33046342	28.26201805	28.04563618	2.596646273	3.482975873	0.977137534
ALANINE M0	99.76797781	94.57893329	94.64477088	95.60551124	99.58931761	94.51120323	94.53137888	94.96079988	98.85995788	94.98278494	95.01965091	94.21891574	99.92369073	94.24657292	94.76683541	95.31214455	99.87569901	94.87086305	94.80493707	95.26300858	99.94914466	96.12719983	95.83079471	95.75545081			99.26305053	99.76874465	99.95166063	99.93241028	99.89375692	99.55216511	75.32155245	74.83980927	74.27260317	75.07679047	75.56922369	74.545826				99.53433134	99.86394289	99.83006885	99.6557165	99.79775639	99.68462477	99.62804726	99.69545941	99.74755773	99.69145124	99.78329625	99.65435663	98.72393243	98.63698507		98.44845351	98.61662155	97.89939993	98.14514825	97.59560883		99.33482324	99.34525669	99.22147188	98.98966303	99.86510382	93.67942522	94.99267059	94.55253019	99.87226928	85.57444703	83.66991033	83.67592215	99.85947947	94.38071931	92.92470559	95.01215461	100	93.96184111	95.69655517	92.42966086	100	83.77534406	81.81309561	86.26278858	100	95.02795707	94.88980375	96.07057799	100	99.97925829	67.99334482	68.00516616	68.10933991	100	97.07626456	100
ALANINE M+1	0.164564809	0.801969478	1.088916153	0.805261459	0.299155022	1.205156508	1.164117265	1.162062594	0.90613493	1.425687907	1.647116525	1.904332241		0.486071653	0.514831604	0.569377262		0.439364958	0.469937295	0.456614973		0.461881192	0.323370588	0.350414991			0.593094559	0.210705182			0.092381902	0.385395626	1.1310542	1.403506708	1.248023419	1.379494423	1.131544178	1.404795286				0.162827445		0.034687413	0.173573248		0.199519926	0.151479462	0.086301334	0.00461826	0.0474136	0.06963813	0.171941404	1.175015457	1.260608247		1.455997887	1.229548111	1.846291758	1.718073801	2.135445635		0.538553899	0.592952861	0.625487496	0.832845793							0.033621353	0.124569673																			0.937575451	1.125628611	1.733299546			
ALANINE M+2	0.045298923	4.607587065	4.255896067	3.569195537	0.103380301	4.275918867	4.304157982	3.874553099	0.233404675	3.583142215	3.328457324	3.871069234		5.238150129	4.671022696	4.063312057		4.624893418	4.694216717	4.243780979		3.389588782	3.81840817	3.866180909			0.13746732	0.017066138				0.05768856	23.52381189	23.74780015	24.46949618	23.53232435	23.28602393	24.04109157																0.072722447	0.083746665		0.095548604	0.123278381	0.254308314	0.136777952	0.26894553					0.089287863		0.785501862	0.56004787	0.655171924		1.33178492	1.534188712	1.552065468		0.697456783	0.97898624	0.662610695						0.367668672	1.214131772	0.801613524		0.47061081	0.162206228				3.054048211	3.019225224	2.963523668			
ALANINE M+3													0.071116234				0.059814091				0.030628767								0.041188838	0.054709836	0.007890373											0.302841212	0.115831164	0.11297062	0.163737862	0.157727177	0.115855299	0.202783059	0.188212463	0.19084854	0.232814242	0.136142591	0.173701967	0.028329663	0.018660015			0.030551963					0.126622862	0.061790448	0.153040627	0.088203315	0.134896175	4.706892077	3.749221574	4.037561807	0.127730723	10.5172902	11.89538218	11.84509716	0.140520526	3.953767746	4.91830912	3.456211573		4.982152281	3.560802949	6.689131682		14.86321557	15.39658347	11.31926924		3.710415178	4.257512587	3.158551978			23.21205784	23.10622647	22.80113546			
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
CITRATE M0
CITRATE M+1
CITRATE M+2
CITRATE M+3
CITRATE M+4
CITRATE M+5
CITRATE M+6
α-KETOGLUTARATE M0
α-KETOGLUTARATE M+1
α-KETOGLUTARATE M+2
α-KETOGLUTARATE M+3
α-KETOGLUTARATE M+4
α-KETOGLUTARATE M+5
GLUTAMATE M0
GLUTAMATE M+1
GLUTAMATE M+2
GLUTAMATE M+3
GLUTAMATE M+4
GLUTAMATE M+5
GLUTAMINE M0
GLUTAMINE M+1
GLUTAMINE M+2
GLUTAMINE M+3
GLUTAMINE M+4
GLUTAMINE M+5
SUCCINATE M0
SUCCINATE M+1
SUCCINATE M+2
SUCCINATE M+3
SUCCINATE M+4
FUMARATE M0
FUMARATE M+1
FUMARATE M+2
FUMARATE M+3
FUMARATE M+4
MALATE M0
MALATE M+1
MALATE M+2
MALATE M+3
MALATE M+4
ASPARTATE M0
ASPARTATE M+1
ASPARTATE M+2
ASPARTATE M+3
ASPARTATE M+4
LACTATE M0
LACTATE M+1
LACTATE M+2
LACTATE M+3
ALANINE M0
ALANINE M+1
ALANINE M+2
ALANINE M+3
METABOLITES_END
#END