#METABOLOMICS WORKBENCH dmw178_20180911_085456_mwtab.txt DATATRACK_ID:1500 STUDY_ID:ST001055 ANALYSIS_ID:AN001724 PROJECT_ID:PR000708
VERSION             	1
CREATED_ON             	September 18, 2018, 12:58 pm
#PROJECT
PR:PROJECT_TITLE                 	Metabolic approaches reveal the role of CAR in energy metabolism
PR:PROJECT_TYPE                  	Time Course
PR:PROJECT_SUMMARY               	The constitutive androstane receptor (CAR; NR1I3) contributes important
PR:PROJECT_SUMMARY               	regulatory roles in biotransformation, xenobiotic transport function, energy
PR:PROJECT_SUMMARY               	metabolism and lipid homeostasis. In this investigation, global serum and liver
PR:PROJECT_SUMMARY               	tissue metabolomes were assessed analytically in wild type and CAR-null
PR:PROJECT_SUMMARY               	transgenic mice using NMR, GC/MS and UPLC/MS-MS-based metabolomics.
PR:PROJECT_SUMMARY               	Significantly, CAR activation increased serum levels of fatty acids, lactate,
PR:PROJECT_SUMMARY               	ketone bodies and tricarboxylic acid cycle products, whereas levels of
PR:PROJECT_SUMMARY               	phosphatidylcholine, sphingomyelin, amino acids and liver glucose were decreased
PR:PROJECT_SUMMARY               	following short-term activation of CAR. Mechanistically, quantitative mRNA
PR:PROJECT_SUMMARY               	analysis demonstrated significantly decreased expression of key gluconeogenic
PR:PROJECT_SUMMARY               	pathways, and increased expression of glucose utilization pathways, changes
PR:PROJECT_SUMMARY               	likely resulting from down-regulation of the hepatic glucose sensor and
PR:PROJECT_SUMMARY               	bi-directional transporter, Glut2. Short-term CAR activation also resulted in
PR:PROJECT_SUMMARY               	enhanced fatty acid synthesis and impaired β-oxidation. In summary, CAR
PR:PROJECT_SUMMARY               	contributes an expansive role regulating energy metabolism, significantly
PR:PROJECT_SUMMARY               	impacting glucose, and monocarboxylic acid, as well as fatty acid metabolism and
PR:PROJECT_SUMMARY               	lipid homeostasis, through receptor-mediated regulation of several genes in
PR:PROJECT_SUMMARY               	multiple associated pathways.
PR:INSTITUTE                     	Pennsylvania State University
PR:LABORATORY                    	Omiecinski Lab
PR:LAST_NAME                     	Omiecinski
PR:FIRST_NAME                    	Curt
PR:ADDRESS                       	101 Life Sciences Building
PR:EMAIL                         	cjo10@psu.edu, dmw178@psu.edu
PR:PHONE                         	8148651572
#STUDY
ST:STUDY_TITLE                   	Fatty acid profiling of liver tissue using GC-MS.
ST:STUDY_TYPE                    	Time Course
ST:STUDY_SUMMARY                 	Liver tissue were harvested from wild type and CAR knockout mice treated for 48
ST:STUDY_SUMMARY                 	or 72h with or without Car agonist (TCPOBOP).
ST:INSTITUTE                     	Pennsylvania State University
ST:LABORATORY                    	Omiecinski Lab
ST:LAST_NAME                     	Omiecinski
ST:FIRST_NAME                    	Curt
ST:ADDRESS                       	101 Life Sciences Building
ST:EMAIL                         	cjo10@psu.edu, dmw178@psu.edu
ST:PHONE                         	8148651572
ST:NUM_GROUPS                    	8
ST:TOTAL_SUBJECTS                	48
ST:NUM_MALES                     	48
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENOTYPE_STRAIN               	Wild Type C57BL/6 and CAR Knockout
SU:AGE_OR_AGE_RANGE              	Approximately 8 weeks old
SU:GENDER                        	Male
SU:ANIMAL_LIGHT_CYCLE            	12 h
SU:ANIMAL_FEED                   	ad libitum
SU:ANIMAL_WATER                  	ad libitum
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	WT_DMSO_48H_101	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):-	File Name=CO140209_22
SUBJECT_SAMPLE_FACTORS           	-	WT_DMSO_48H_102	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):-	File Name=CO140209_32
SUBJECT_SAMPLE_FACTORS           	-	WT_DMSO_48H_103	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):-	File Name=CO141205_42
SUBJECT_SAMPLE_FACTORS           	-	WT_DMSO_48H_104	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):-	File Name=CO141205_47
SUBJECT_SAMPLE_FACTORS           	-	WT_DMSO_48H_105	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):-	File Name=CO141205_52
SUBJECT_SAMPLE_FACTORS           	-	WT_DMSO_48H_106	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):-	File Name=CO141205_67
SUBJECT_SAMPLE_FACTORS           	-	WT_DMSO_48H_107	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):-	File Name=CO141205_79
SUBJECT_SAMPLE_FACTORS           	-	WT_TCP_48H_201	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):2	File Name=CO140209_06
SUBJECT_SAMPLE_FACTORS           	-	WT_TCP_48H_202	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):2	File Name=CO140209_21
SUBJECT_SAMPLE_FACTORS           	-	WT_TCP_48H_203	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):2	File Name=CO140209_24
SUBJECT_SAMPLE_FACTORS           	-	WT_TCP_48H_204	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):2	File Name=CO141205_76
SUBJECT_SAMPLE_FACTORS           	-	WT_TCP_48H_205	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):2	File Name=CO141205_78
SUBJECT_SAMPLE_FACTORS           	-	WT_TCP_48H_206	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):2	File Name=CO141205_23
SUBJECT_SAMPLE_FACTORS           	-	WT_DMSO_72H_301	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):-	File Name=CO140209_26
SUBJECT_SAMPLE_FACTORS           	-	WT_DMSO_72H_302	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):-	File Name=CO140209_27
SUBJECT_SAMPLE_FACTORS           	-	WT_DMSO_72H_303	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):-	File Name=CO140209_29
SUBJECT_SAMPLE_FACTORS           	-	WT_DMSO_72H_304	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):-	File Name=CO140209_40
SUBJECT_SAMPLE_FACTORS           	-	WT_DMSO_72H_305	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):-	File Name=CO141205_53
SUBJECT_SAMPLE_FACTORS           	-	WT_DMSO_72H_306	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):-	File Name=CO141205_71
SUBJECT_SAMPLE_FACTORS           	-	WT_TCP_72H_401	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):2	File Name=CO140209_13
SUBJECT_SAMPLE_FACTORS           	-	WT_TCP_72H_402	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):2	File Name=CO140209_33
SUBJECT_SAMPLE_FACTORS           	-	WT_TCP_72H_403	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):2	File Name=CO141205_55
SUBJECT_SAMPLE_FACTORS           	-	WT_TCP_72H_404	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):2	File Name=CO141205_57
SUBJECT_SAMPLE_FACTORS           	-	WT_TCP_72H_405	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):2	File Name=CO141205_62
SUBJECT_SAMPLE_FACTORS           	-	WT_TCP_72H_406	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):2	File Name=CO141205_69
SUBJECT_SAMPLE_FACTORS           	-	CARKO_DMSO_48H_101	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):-	File Name=CO140209_05
SUBJECT_SAMPLE_FACTORS           	-	CARKO_DMSO_48H_102	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):-	File Name=CO140209_28
SUBJECT_SAMPLE_FACTORS           	-	CARKO_DMSO_48H_103	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):-	File Name=CO140209_30
SUBJECT_SAMPLE_FACTORS           	-	CARKO_DMSO_48H_104	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):-	File Name=CO141205_41
SUBJECT_SAMPLE_FACTORS           	-	CARKO_DMSO_48H_105	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):-	File Name=CO141205_46
SUBJECT_SAMPLE_FACTORS           	-	CARKO_DMSO_48H_106	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):-	File Name=CO141205_58
SUBJECT_SAMPLE_FACTORS           	-	CARKO_TCP_48H_201	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):2	File Name=CO140209_09
SUBJECT_SAMPLE_FACTORS           	-	CARKO_TCP_48H_202	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):2	File Name=CO140209_14
SUBJECT_SAMPLE_FACTORS           	-	CARKO_TCP_48H_203	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):2	File Name=CO140209_35
SUBJECT_SAMPLE_FACTORS           	-	CARKO_TCP_48H_204	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):2	File Name=CO140209_36
SUBJECT_SAMPLE_FACTORS           	-	CARKO_TCP_48H_205	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):2	File Name=CO141205_50
SUBJECT_SAMPLE_FACTORS           	-	CARKO_TCP_48H_206	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):2	File Name=CO141205_56
SUBJECT_SAMPLE_FACTORS           	-	CARKO_TCP_48H_207	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):2	File Name=CO141205_59
SUBJECT_SAMPLE_FACTORS           	-	CARKO_DMSO_72H_301	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):-	File Name=CO140209_07
SUBJECT_SAMPLE_FACTORS           	-	CARKO_DMSO_72H_302	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):-	File Name=CO140209_38
SUBJECT_SAMPLE_FACTORS           	-	CARKO_DMSO_72H_303	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):-	File Name=CO141205_64
SUBJECT_SAMPLE_FACTORS           	-	CARKO_DMSO_72H_304	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):-	File Name=CO141205_66
SUBJECT_SAMPLE_FACTORS           	-	CARKO_DMSO_72H_305	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):-	File Name=CO141205_75
SUBJECT_SAMPLE_FACTORS           	-	CARKO_DMSO_72H_306	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):-	File Name=CO141205_81
SUBJECT_SAMPLE_FACTORS           	-	CARKO_TCP_72H_401	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):2	File Name=CO140209_08
SUBJECT_SAMPLE_FACTORS           	-	CARKO_TCP_72H_402	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):2	File Name=CO140209_12
SUBJECT_SAMPLE_FACTORS           	-	CARKO_TCP_72H_403	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):2	File Name=CO140209_31
SUBJECT_SAMPLE_FACTORS           	-	CARKO_TCP_72H_404	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):2	File Name=CO140209_37
SUBJECT_SAMPLE_FACTORS           	-	CARKO_TCP_72H_405	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):2	File Name=CO141205_51
SUBJECT_SAMPLE_FACTORS           	-	CARKO_TCP_72H_406	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):2	File Name=CO141205_80
#COLLECTION
CO:COLLECTION_SUMMARY            	Liver tissue was snap-frozen in liquid nitrogen and stored at -80C.
CO:SAMPLE_TYPE                   	Liver
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	Each mouse was treated with either a single does of 2 mg/kg of TCPOBOP or the
TR:TREATMENT_SUMMARY             	vehicle control via intraperitoneal injection for either 48 or 72h.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Liver tissue was homogenized in a methanol-water mixture, dried and resuspended
SP:SAMPLEPREP_SUMMARY            	in phosphate buffer containing TSP-d4.
SP:SAMPLEPREP_PROTOCOL_FILENAME  	GCMS_FattyAcid_protocol.pdf
SP:PROCESSING_METHOD             	Homogenization
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	GC
MS:INSTRUMENT_NAME               	Agilent 5975C
CH:COLUMN_NAME                   	Agilent HP-5MS
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:MS_COMMENTS                   	-
MS:INSTRUMENT_NAME               	Agilent 5975C
MS:INSTRUMENT_TYPE               	Single quadrupole
MS:MS_TYPE                       	EI
MS:ION_MODE                      	POSITIVE
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	Peak Area
MS_METABOLITE_DATA_START
Samples	WT_DMSO_48H_101	WT_DMSO_48H_102	WT_DMSO_48H_103	WT_DMSO_48H_104	WT_DMSO_48H_105	WT_DMSO_48H_106	WT_DMSO_48H_107	WT_TCP_48H_201	WT_TCP_48H_202	WT_TCP_48H_203	WT_TCP_48H_204	WT_TCP_48H_205	WT_TCP_48H_206	WT_DMSO_72H_301	WT_DMSO_72H_302	WT_DMSO_72H_303	WT_DMSO_72H_304	WT_DMSO_72H_305	WT_DMSO_72H_306	WT_TCP_72H_401	WT_TCP_72H_402	WT_TCP_72H_403	WT_TCP_72H_404	WT_TCP_72H_405	WT_TCP_72H_406	CARKO_DMSO_48H_101	CARKO_DMSO_48H_102	CARKO_DMSO_48H_103	CARKO_DMSO_48H_104	CARKO_DMSO_48H_105	CARKO_DMSO_48H_106	CARKO_TCP_48H_201	CARKO_TCP_48H_202	CARKO_TCP_48H_203	CARKO_TCP_48H_204	CARKO_TCP_48H_205	CARKO_TCP_48H_206	CARKO_TCP_48H_207	CARKO_DMSO_72H_301	CARKO_DMSO_72H_302	CARKO_DMSO_72H_303	CARKO_DMSO_72H_304	CARKO_DMSO_72H_305	CARKO_DMSO_72H_306	CARKO_TCP_72H_401	CARKO_TCP_72H_402	CARKO_TCP_72H_403	CARKO_TCP_72H_404	CARKO_TCP_72H_405	CARKO_TCP_72H_406
Factors	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):-	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):-	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):-	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):-	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):-	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):-	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):-	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):2	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):2	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):2	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):2	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):2	Genotype:WT | Time Point (h):48 | TCPOBOP (mg/kg):2	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):-	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):-	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):-	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):-	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):-	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):-	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):2	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):2	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):2	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):2	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):2	Genotype:WT | Time Point (h):72 | TCPOBOP (mg/kg):2	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):-	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):-	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):-	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):-	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):-	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):-	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):2	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):2	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):2	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):2	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):2	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):2	Genotype:CARKO | Time Point (h):48 | TCPOBOP (mg/kg):2	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):-	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):-	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):-	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):-	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):-	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):-	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):2	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):2	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):2	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):2	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):2	Genotype:CARKO | Time Point (h):72 | TCPOBOP (mg/kg):2
C15:0	298002	2169285	401558	2846173	2363225	2370960	2315368	1420726	1571101	2605398	572968	1567058	326303	3262419	4563808	293912	1772131	2144537	2876619	600250	2652921	3020841	1850781	2290440	2249687	1065243	3212182	429300	2293573	2349115	2469412	1660931	1515248	1779360	2577935	2388719	1496157	2046757	1895248	4101207	2701342	963071	1328576	1681367	1688309	2637566	2143972	959360	152847	3348280
C16:1n9		218425	62070	523693	318475	298855	626314	595936	393556	821749	158192	620204	53694	624529	1029721	38270	268850	406902	603224	300657	1686269	1352630	661923	1535825	815525	140132	539123	66178	533107	418762	379818	473421	207427	346735	650573	605135	247102	312251	648727	916660	1076357	265267	284561	285776	432572	392121	575295	246638		826129
C16:1n7	199150	1161178	361633	2441495	1801189	1481993	1106490	2057892	888409	2131134	332647	1267171	210007	3262432	6160393	350381	1261614	2106577	3250746	416115	2205284	2025978	1069965	1920896	1204251	1207686	3694671	484918	3080376	2877767	2799916	1951164	1092252	1566716	3298255	2030492	1344648	2219368	2490695	3972804	5378924	1288661	1515575	1304542	2122134	1730241	2817795	1407270	144751	3229097
C16:0	10934804	68517769	16965308	85122118	79849231	70324671	76645817	56170673	57664205	101892987	27062933	49181230	16925350	95090082	147570293	14792758	58498328	65261417	84427169	29473449	118289653	112055594	68676005	72363510	70062045	33577858	103786177	20307720	73804703	67483178	82645081	61693721	57643774	53663390	99338175	73466240	56660703	67278606	76823756	115921741	101766522	31448086	43873358	55271111	57028074	81802508	75327754	40899452	8891075	116244868
C17:0	252418	1582572	338999	1809755	1530621	1677053	1587562	1064030	1220778	1967959	525208	1041692	314445	2149351	2664788	259939	1347079	1372742	1707597	600484	2180302	2251334	1518141	1511578	1542303	669018	1962391	322118	1519737	1238538	1552579	1131384	1064642	1067558	1737459	1488739	1045812	1293560	1228659	2473537	1727847	629185	855583	1161812	1084295	1783599	1339859	678555	142809	2500424
C18:3		248406		312351	221586	247020	107672	356408	439117	626499	121175	347247	57251	384035	498144		310706	308395	421266	161040	1027855	1022665	493634	449694	552794	62507	255638		208861	258448	292983	153590	107904	121766	262326	213444	152050	144604	178795	393386	254563	62885	151526	134461	105247	199191	240099	65472		389809
C18:2	6693336	43609512	9582493	53641217	48644621	46002138	50113062	37398611	44866310	79685588	18790607	36176438	11686641	53980391	83845807	7605718	33668387	40215556	46352117	21976265	98827328	87190283	52778156	65673619	53347270	20490015	55890396	11009139	44519780	40927017	48803203	37864410	33876951	32592825	59733135	50630392	34223530	41659869	43990493	71985595	59817161	18457274	27965267	33475704	33010179	53117805	43834994	21479250	5487639	78362098
C18:1n9	4316737	20476819	6636232	35995890	27170768	25653849	29838451	28650829	27631849	50455284	11777369	27709630	7263844	33970716	59524827	5949133	18412242	25955354	32282586	15717403	59264130	57723646	35313492	58919680	34658902	13538504	40542428	8009083	35291368	26036224	32781722	26094640	21141209	22032434	40846905	30311301	21356492	29294348	33553573	49519290	51021602	15400967	18531046	20131985	22973951	27079893	30248947	17474454	3668325	47065699
C18:1n11	450373	2728763	764162	4102353	3655194	3254862	3489691	2434990	2318503	3831148	780543	2049941	607829	4476961	8517957	704430	2081876	2914977	3863647	1086556	3859072	3805977	2732144	4984008	2336959	1968952	6284073	1090639	5210486	3135227	4492813	3468746	2399336	2815227	6056859	4017640	2599471	4632826	4404182	6837640	8636382	2349476	2248863	3038453	3580349	4489063	4221811	2512164	500339	7398113
C18:0	12501434	59111961	17275384	67212112	61573133	53699792	54677220	40342264	60998907	75032976	28294860	40995526	20526030	65481095	97704816	14411752	45620570	48433811	52884201	36235580	110051049	103859351	68181977	85669059	63627854	22183893	62876416	19897803	52864929	43847294	53880539	40938635	37444639	34722916	66053615	52110715	40147668	50594564	48722212	74180170	54835511	24040010	30597601	47358188	35038760	61800561	48257599	27950314	9259102	85944143
C20:4	5185384	37052663	8079371	50327048	45932879	40234001	40083418	22292279	28470589	38229086	9923819	22333907	6175210	49437847	75761876	6900772	31061689	35869558	40033163	13535313	57666750	53089759	32197774	42316803	33448885	16810765	47043280	9454989	34474077	30708185	37352191	30784068	26402714	26778587	48350507	40016981	27232554	34745459	40147912	61249558	46959630	16629878	23734465	33097139	27915386	388784	36887820	17294645	3914529	69724638
C20:5	243436	2528796	477344	3947395	3290081	2989331	2944527	2043779	2850483	3568893	597207	1707388	413676	3856061	6830254	314847	2034543	2458713	3427293	648371	3670339	3706792	2434748	3100929	1968476	1618679	6101053	756352	3646355	3047450	4181665	1829748	1594082	1675350	4060521	2599762	2112630	3349632	2028529	4336700	4000233	1027021	1372625	2421645	1821628	46463658	3998272	912782	257281	3840091
C20:3	436410	3269352	660069	4653770	3494181	4071014	1962198	2017724	2370225	3372648	742363	1858816	412449	4042274	6432520	597777	2573942	3231807	4012832	1107147	4636555	4280941	2518093	3858367	2535743	1628931	5333750	829683	3829945	3149110	4349014	2783965	2293785	2374389	4641808	3383513	2266601	3474923	3583946	6393043	5014854	1459219	2034249	3096830	3125459	3883836	3505120	1603230	217272	5818341
C20:2	56001	466747	65549	602644	494610	628332	649594	348238	400076	843012	160030	305747	80072	612790	1036417	81961	353254	380846	537907	194710	916082	800059	480724	557631	450617	215422	765631	115778	552550	360690	584724	439079	438268	350630	726640	564581	391069	535253	529049	881976	857619	188892	292052	405738	398130	3668233	509883	249621	38151	1038479
C20:1	78022	475094	116452	758311	529912	775149	825977	601954	671145	1340968	360951	674367	129538	834141	1183784	125133	461679	543859	777809	482799	1613988	1359094	799246	1250267	927938	352504	993036	153205	896506	674218	804794	549778	466743	495327	972947	771154	534130	601704	805637	1432802	1241768	353328	506348	574320	574860	754319	793372	441351	60867	1333001
C20:0	222870	883620	316118	972292	1007891	1303142	820076	786458	835585	1529636	611813	627402	313807	1250620	1679530	323401	1127728	960626	1170095	751393	1658304	1484306	913913	819900	855848	413061	1240014	447530	1040989	991413	1147921	710119	658776	520950	1053938	799476	790389	504975	922231	1699623	839614	455742	668485	831784	762435	776752	944410	739855	130772	1592605
C22:6	3509154	30988212	6218392	42447737	41007682	35239745	48661186	19324676	26349018	44043931	8773357	18556447	4454200	42207772	68510032	4900403	30134274	33370962	41494499	10165628	47993576	49864124	32490109	29205883	30224744	13340393	51510715	9083170	32474379	30694421	40548782	30294794	29618717	24015472	43953767	37231295	28056157	33906114	39241231	44953246	46745110	12361036	19062117	28684894	25043256	964115	37972948	14217904	2370470	60178444
C22:4	123256	1138944	259145	2054395	2005571	1482659	2215506	987421	1211398	2620363	409508	858415	144937	2045854	4356321	236697	1426890	1542033	2148298	415723	2392714	2445040	1922672	1320292	1738949	515454	2835197	382536	1686797	1451586	1911571	1035451	1260455	1020342	2184560	1586009	1273095	1387307	1627371	1981390	2489906	535423	748190	1191268		43142342	1831775	666715	96948	2082034
C22:0	900838	2899205	1273303	2953614	3135826	2747176	2903642	1364046	1735454	2562460	1518777	1156407	935793	2941654	4225992	1204068	2780376	2348768	2578452	1440560	2874616	2802058	1973642	1710911	1614738	1172901	3528731	1787539	2700795	2852669	3250292	1972963	2483511	1837777	3342018	2736005	2887957	1876204	2501617	3946679	2791318	1512399	1871648	2225045	2189172	1628506	2478691	2272177	628340	4265943
C23:0	154217	496707	203330	447906	399633	435585	379161	157839	215920	274964	149724	120222	136914	457165	593800	153512	332561	262003	330782	139943	263301	286405	218002	202816	131332	173019	544814	198639	518182	279142	466565	277147	364543	221641	460647	393489	389863	558861	295764	447574	532713	265264	241727	392320	249207	3138179	263521	308073	169474	778200
C24:1	429158	1418518	565657	1728710	1816361	1539669	2087082	690713	743878	1044350	581667	614324	434010	1566188	2410993	560824	883442	1065197	1321508	527731	1495502	1278156	942740	1454577	836674	744630	2170905	934769	1895752	1076699	1625326	1518897	1130023	1074764	1963545	1801864	1162163	2131432	1675821	2299447	2698192	1107172	1050724	1772662	1153049	2235027	1385579	1266637	441426	3431145
C24:0	615794	1672615	985233	1650622	1956131	1674217	1655062	1001507	1212870	1827979	1172215	710354	900731	1867789	2678545	852675	1809922	1305438	1818247	1375893	2045346	2025973	1524988	1453924	1231295	561029	1846470	1088856	1723629	975507	1706956	930931	1247033	768768	1590797	1406330	1271672	1480121	1137330	1531668	1730435	898972	837976	1227216	909762	1517374	990270	1212875	522774	2092779
cholesterol	1533921	3512838	2334891	3702663	3788611	2918460	3150091	1881442	1980578	3196430	1840125	1706329	1557625	3651906	4865077	2161938	2881988	2457493	3188773	2185744	4198271	3529977	2574851	3460931	2281863	2288120	4118936	3015179	3889620	2943588	3058714	2117573	3116050	2338892	3979685	3278741	3139334	3734966	3413188	4357331	4415115	2451736	2848361	3794968	2752084	4648151	2807626	3092067	1980385	5909826
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
C15:0
C16:1n9
C16:1n7
C16:0
C17:0
C18:3
C18:2
C18:1n9
C18:1n11
C18:0
C20:4
C20:5
C20:3
C20:2
C20:1
C20:0
C22:6
C22:4
C22:0
C23:0
C24:1
C24:0
cholesterol
METABOLITES_END
#END