#METABOLOMICS WORKBENCH fernandezlab_20181019_132548 DATATRACK_ID:1553 STUDY_ID:ST001083 ANALYSIS_ID:AN001768 PROJECT_ID:PR000724 VERSION 1 CREATED_ON October 29, 2018, 7:38 pm #PROJECT PR:PROJECT_TITLE Preoperative Metabolic Signatures of Prostate Cancer Recurrence Following PR:PROJECT_TITLE Radical Prostatectomy PR:PROJECT_SUMMARY Serum of two groups of patients were analyzed using MS and NMR to find PR:PROJECT_SUMMARY predictive markers of recurrence PR:INSTITUTE Georgia Institute of Technology PR:LAST_NAME Fernandez PR:FIRST_NAME Facundo PR:ADDRESS 901 Atlantic Dr NE, Atlanta, GA, 30332, USA PR:EMAIL fernandez@gatech.edu PR:PHONE 404-385-4432 #STUDY ST:STUDY_TITLE Combined NMR and MS Analysis of PC patien serum (part-III) ST:STUDY_SUMMARY Serum of two groups of patients were analyzed using MS and NMR to find ST:STUDY_SUMMARY predictive markers of recurrence. Study Design factor Sample type represents ST:STUDY_SUMMARY samples with Recurrence (R) or No Recurrence (NR) of Cancer. ST:INSTITUTE Georgia Institute of Technology ST:LAST_NAME Fernandez ST:FIRST_NAME Facundo ST:ADDRESS 901 Atlantic Dr NE, Atlanta, GA, 30332, USA ST:EMAIL fernandez@gatech.edu ST:PHONE 404-385-4432 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:AGE_OR_AGE_RANGE 44-70 SU:GENDER Male #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - S1 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S2 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S3 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S4 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S5 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S9 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S10 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S11 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S12 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S15 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S16 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S17 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S18 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S19 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S20 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S21 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S22 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S24 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S25 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S26 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S27 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S28 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S29 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S30 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S31 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S32 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S33 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S35 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S36 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S37 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S38 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S39 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S41 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S42 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S43 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S44 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S45 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S46 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S47 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S48 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S49 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S50 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S51 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S52 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S54 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S57 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S58 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S59 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S60 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S61 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S62 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S63 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S64 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S65 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S66 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S67 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S68 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S69 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S71 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S72 Sample type:No Recurrence SUBJECT_SAMPLE_FACTORS - S73 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S74 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S75 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S76 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S78 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S79 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S80 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S82 Sample type:Recurrence SUBJECT_SAMPLE_FACTORS - S83 Sample type:Recurrence #COLLECTION CO:COLLECTION_SUMMARY After Emory Institutional Review Board (IRB) approval, patients were consented CO:COLLECTION_SUMMARY and blood collected in SST Serum Separation Tubes: Hemogard (Fisher Scientific). CO:COLLECTION_SUMMARY Serum was separated by centrifugation at 1300 rcf for 20 min at 4 °C. Serum CO:COLLECTION_SUMMARY aliquots were stored long term in liquid nitrogen until removed for analysis. CO:SAMPLE_TYPE Blood (serum) CO:STORAGE_CONDITIONS Described in summary #TREATMENT TR:TREATMENT_SUMMARY N/A #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Proteins were precipitated from serum samples using either MeOH (for HILIC LC/MS SP:SAMPLEPREP_SUMMARY and NMR) or Isopropanol (for RP LC/MS). #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE - CH:INSTRUMENT_NAME - CH:COLUMN_NAME - #ANALYSIS AN:ANALYSIS_TYPE NMR #NMR NM:INSTRUMENT_NAME Bruker Avance III HD 600 MHz NM:INSTRUMENT_TYPE FT-NMR NM:NMR_EXPERIMENT_TYPE 1D-1H NM:SPECTROMETER_FREQUENCY 600 MHz NM:NMR_SOLVENT D2O NM:NMR_TUBE_SIZE 5 mm NM:NMR_RESULTS_FILE ST001083_AN001768_Results.txt UNITS:ppm #END