#METABOLOMICS WORKBENCH kenanazam_20160913_121032 DATATRACK_ID:739 STUDY_ID:ST000471 ANALYSIS_ID:AN001822 VERSION 1 CREATED_ON 08-09-2023 #PROJECT PR:PROJECT_TITLE Degradation of Nucleotides under different chemical environments PR:PROJECT_SUMMARY The degradation kinetics of nucleotide triphosphates (ATP, GTP, UTP and CTP) PR:PROJECT_SUMMARY were evaluated under boiling ethanol extraction conditions (95°C) during 0 to PR:PROJECT_SUMMARY 300 minutes. PR:INSTITUTE University of Groningen PR:DEPARTMENT Analytical Biochemistry PR:LAST_NAME Bischoff PR:FIRST_NAME Rainer PR:ADDRESS Antonius Deusinglaan 1 (XB20), Building 3226, room 601, 9713 AV Groningen, The PR:ADDRESS Netherlands PR:EMAIL r.p.h.bischoff@rug.nl PR:PHONE NA PR:PUBLICATIONS Gil, A., Siegel, D., Bonsing-Vedelaar, S. et al. The degradation of nucleotide PR:PUBLICATIONS triphosphates extracted under boiling ethanol conditions is prevented by the PR:PUBLICATIONS yeast cellular matrix. Metabolomics (2017) 13:1. doi:10.1007/s11306-016-1140-4 PR:DOI http://dx.doi.org/10.21228/M8CW21 #STUDY ST:STUDY_TITLE Effect of a complex matrix on the degradation of nucleotide triphosphates ST:STUDY_TYPE Comparison of degradation kinetics ST:STUDY_SUMMARY The effect of a complex cellular matrix extracted from yeast (S. cerevisiae, ST:STUDY_SUMMARY strain YSBN6 (MATa; genotype: FY3 ho::HphMX4 derived from the S288C parental ST:STUDY_SUMMARY strain)) on the degradation profiles of nucleotide triphosphates extracted under ST:STUDY_SUMMARY typical boiling ethanol conditions was evaluated. ST:INSTITUTE University of Groningen ST:DEPARTMENT Analytical Biochemistry ST:LAST_NAME Bischoff ST:FIRST_NAME Rainer ST:ADDRESS Antonius Deusinglaan 1 (XB20), Building 3226, room 601, 9713 AV Groningen, The ST:ADDRESS Netherlands ST:EMAIL r.p.h.bischoff@rug.nl ST:PHONE NA ST:SUBMIT_DATE 2016-09-13 #SUBJECT SU:SUBJECT_TYPE Chemical SU:SUBJECT_SPECIES None SU:SUBJECT_COMMENTS Primary standards of nucleotide triphosphates (ATP, GTP, UTP and CTP). #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - AG140814_27 Time (mins):12 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_28 Time (mins):12 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_29 Time (mins):12 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_30 Time (mins):12 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG130814_62 Time (mins):12 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_63 Time (mins):12 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_64 Time (mins):12 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_65 Time (mins):12 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_27 Time (mins):12 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_28 Time (mins):12 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_29 Time (mins):12 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_30 Time (mins):12 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_62 Time (mins):12 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_63 Time (mins):12 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_64 Time (mins):12 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_65 Time (mins):12 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_27 Time (mins):12 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_28 Time (mins):12 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_29 Time (mins):12 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_30 Time (mins):12 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_62 Time (mins):12 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_63 Time (mins):12 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_64 Time (mins):12 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_65 Time (mins):12 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_27 Time (mins):12 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_28 Time (mins):12 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_29 Time (mins):12 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_30 Time (mins):12 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_62 Time (mins):12 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_63 Time (mins):12 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_64 Time (mins):12 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_65 Time (mins):12 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG140814_32 Time (mins):15 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_33 Time (mins):15 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_34 Time (mins):15 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_35 Time (mins):15 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG130814_67 Time (mins):15 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_68 Time (mins):15 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_69 Time (mins):15 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_70 Time (mins):15 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_32 Time (mins):15 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_33 Time (mins):15 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_34 Time (mins):15 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_35 Time (mins):15 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_67 Time (mins):15 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_68 Time (mins):15 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_69 Time (mins):15 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_70 Time (mins):15 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_32 Time (mins):15 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_33 Time (mins):15 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_34 Time (mins):15 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_35 Time (mins):15 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_67 Time (mins):15 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_68 Time (mins):15 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_69 Time (mins):15 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_70 Time (mins):15 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_32 Time (mins):15 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_33 Time (mins):15 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_34 Time (mins):15 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_35 Time (mins):15 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_67 Time (mins):15 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_68 Time (mins):15 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_69 Time (mins):15 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_70 Time (mins):15 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG140814_12 Time (mins):3 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_13 Time (mins):3 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_14 Time (mins):3 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_15 Time (mins):3 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG130814_47 Time (mins):3 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_48 Time (mins):3 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_49 Time (mins):3 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_50 Time (mins):3 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_12 Time (mins):3 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_13 Time (mins):3 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_14 Time (mins):3 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_15 Time (mins):3 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_47 Time (mins):3 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_48 Time (mins):3 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_49 Time (mins):3 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_50 Time (mins):3 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_12 Time (mins):3 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_13 Time (mins):3 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_14 Time (mins):3 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_15 Time (mins):3 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_47 Time (mins):3 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_48 Time (mins):3 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_49 Time (mins):3 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_50 Time (mins):3 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_12 Time (mins):3 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_13 Time (mins):3 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_14 Time (mins):3 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_15 Time (mins):3 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_47 Time (mins):3 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_48 Time (mins):3 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_49 Time (mins):3 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_50 Time (mins):3 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG140814_17 Time (mins):6 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_18 Time (mins):6 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_19 Time (mins):6 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_20 Time (mins):6 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG130814_52 Time (mins):6 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_53 Time (mins):6 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_54 Time (mins):6 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_55 Time (mins):6 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_17 Time (mins):6 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_18 Time (mins):6 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_19 Time (mins):6 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_20 Time (mins):6 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_52 Time (mins):6 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_53 Time (mins):6 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_54 Time (mins):6 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_55 Time (mins):6 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_17 Time (mins):6 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_18 Time (mins):6 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_19 Time (mins):6 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_20 Time (mins):6 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_52 Time (mins):6 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_53 Time (mins):6 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_54 Time (mins):6 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_55 Time (mins):6 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_17 Time (mins):6 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_18 Time (mins):6 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_19 Time (mins):6 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_20 Time (mins):6 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_52 Time (mins):6 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_53 Time (mins):6 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_54 Time (mins):6 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_55 Time (mins):6 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG140814_22 Time (mins):9 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_23 Time (mins):9 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_24 Time (mins):9 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_25 Time (mins):9 | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG130814_57 Time (mins):9 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_58 Time (mins):9 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_59 Time (mins):9 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_60 Time (mins):9 | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_22 Time (mins):9 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_23 Time (mins):9 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_24 Time (mins):9 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_25 Time (mins):9 | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_57 Time (mins):9 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_58 Time (mins):9 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_59 Time (mins):9 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_60 Time (mins):9 | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_22 Time (mins):9 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_23 Time (mins):9 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_24 Time (mins):9 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_25 Time (mins):9 | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_57 Time (mins):9 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_58 Time (mins):9 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_59 Time (mins):9 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_60 Time (mins):9 | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_22 Time (mins):9 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_23 Time (mins):9 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_24 Time (mins):9 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_25 Time (mins):9 | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_57 Time (mins):9 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_58 Time (mins):9 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_59 Time (mins):9 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_60 Time (mins):9 | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG140814_10 Time (mins):- | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_7 Time (mins):- | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_8 Time (mins):- | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG140814_9 Time (mins):- | Nucleotide:ATP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG130814_42 Time (mins):- | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_43 Time (mins):- | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_44 Time (mins):- | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG130814_45 Time (mins):- | Nucleotide:ATP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_10 Time (mins):- | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_7 Time (mins):- | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_8 Time (mins):- | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_9 Time (mins):- | Nucleotide:CTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG251114_42 Time (mins):- | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_43 Time (mins):- | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_44 Time (mins):- | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG251114_45 Time (mins):- | Nucleotide:CTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_10 Time (mins):- | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_7 Time (mins):- | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_8 Time (mins):- | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_9 Time (mins):- | Nucleotide:GTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG271014_42 Time (mins):- | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_43 Time (mins):- | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_44 Time (mins):- | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG271014_45 Time (mins):- | Nucleotide:GTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_10 Time (mins):- | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_7 Time (mins):- | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_8 Time (mins):- | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_9 Time (mins):- | Nucleotide:UTP | Yeast Matrix:No SUBJECT_SAMPLE_FACTORS - AG261114_42 Time (mins):- | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_43 Time (mins):- | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_44 Time (mins):- | Nucleotide:UTP | Yeast Matrix:Yes SUBJECT_SAMPLE_FACTORS - AG261114_45 Time (mins):- | Nucleotide:UTP | Yeast Matrix:Yes #COLLECTION CO:COLLECTION_SUMMARY None #TREATMENT TR:TREATMENT_SUMMARY Pure solutions of nucleotide triphosphates (ATP, GTP, CTP and UTP) were TR:TREATMENT_SUMMARY incubated under boiling ethanol conditions (95°C) during 0, 5, 10, 20, 30, 60, TR:TREATMENT_SUMMARY 120, 180, 240 and 300 minutes. TR:TREATMENT Abiotic #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Tubes containing 496 µL of 75% (aq) ethanol were preheated at 95°C for 5 min, SP:SAMPLEPREP_SUMMARY followed by the addition of 4 µL of each nucleotide standard solution (500 µM) SP:SAMPLEPREP_SUMMARY and vigorous mixing. 4 µL of solutions “A” to “H” were added to the SP:SAMPLEPREP_SUMMARY reaction mixture and the samples were incubated at 95°C under shaking for 0 and SP:SAMPLEPREP_SUMMARY 15 min. Reactions were stopped by snap-freezing in liquid nitrogen and samples SP:SAMPLEPREP_SUMMARY were stored on dry ice. Subsequently, samples were thawed and 20 µL of the SP:SAMPLEPREP_SUMMARY 13C15N-labeled internal standard solution were added for quantitative analysis. SP:SAMPLEPREP_SUMMARY Excess solvent was evaporated under a stream of nitrogen without heating. SP:SAMPLEPREP_SUMMARY Finally, samples were reconstituted in 200 µL of acetonitrile-water 70:30 and SP:SAMPLEPREP_SUMMARY stored at –40°C until analysis by LC-MS. After the experimental procedure the SP:SAMPLEPREP_SUMMARY final concentration of all components in solutions “A” to “H” was 10 SP:SAMPLEPREP_SUMMARY µM. Compounds to be tested were divided into eight groups containing SP:SAMPLEPREP_SUMMARY approximately ten compounds each, mainly comprising major central carbon SP:SAMPLEPREP_SUMMARY metabolites including amino acids, organic acids, sugar phosphates, coenzymes, SP:SAMPLEPREP_SUMMARY etc (Table 1). SP:PROCESSING_METHOD Preparation of reagents, dilution, solvent removal under a stream of nitrogen SP:PROCESSING_METHOD without heating and reconstitution SP:EXTRACTION_METHOD Boiling ethanol (95°C) SP:EXTRACT_ENRICHMENT Evaporation of excess of solvent under a stream of nitrogen without heating. SP:EXTRACT_STORAGE -40C SP:SAMPLE_RESUSPENSION 200 µL of acetonitrile-water 70:30 #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Untargeted HILIC Method CH:INSTRUMENT_NAME Waters Acquity CH:COLUMN_NAME Phenomenex Luna NH2 (100 x 2.0mm,3um) CH:COLUMN_TEMPERATURE 20C CH:FLOW_GRADIENT 30% eluent A to 99% eluent A in 8 min, followed by isocratic elution at 99% CH:FLOW_GRADIENT eluent A until 14 min. A conditioning cycle of 6 min with the initial CH:FLOW_GRADIENT proportions of eluents A and B was performed prior to the next analysis. CH:FLOW_RATE 0.25 mL/min CH:SAMPLE_INJECTION 10 µL CH:SOLVENT_A 100% water; 5 mM ammonium acetate, pH 9.9 CH:SOLVENT_B 100% acetonitrile CH:ANALYTICAL_TIME 20 min CH:CHROMATOGRAPHY_TYPE HILIC #ANALYSIS AN:ANALYSIS_TYPE MS AN:SOFTWARE_VERSION MassLynx 4.1 #MS MS:INSTRUMENT_NAME Waters Synapt G2 Si QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:CAPILLARY_VOLTAGE 2KV MS:COLLISION_ENERGY 2 V for the low-collision energy scan, and 10–30 V for the high-collision MS:COLLISION_ENERGY energy scan MS:DRY_GAS_FLOW Argon MS:SOURCE_TEMPERATURE 150C MS:DESOLVATION_TEMPERATURE 400C MS:SCAN_RANGE_MOVERZ 50 to 1200 Da #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Peak area MS_METABOLITE_DATA_START Samples AG140814_27 AG140814_28 AG140814_29 AG140814_30 AG130814_62 AG130814_63 AG130814_64 AG130814_65 AG251114_27 AG251114_28 AG251114_29 AG251114_30 AG251114_62 AG251114_63 AG251114_64 AG251114_65 AG271014_27 AG271014_28 AG271014_29 AG271014_30 AG271014_62 AG271014_63 AG271014_64 AG271014_65 AG261114_27 AG261114_28 AG261114_29 AG261114_30 AG261114_62 AG261114_63 AG261114_64 AG261114_65 AG140814_32 AG140814_33 AG140814_34 AG140814_35 AG130814_67 AG130814_68 AG130814_69 AG130814_70 AG251114_32 AG251114_33 AG251114_34 AG251114_35 AG251114_67 AG251114_68 AG251114_69 AG251114_70 AG271014_32 AG271014_33 AG271014_34 AG271014_35 AG271014_67 AG271014_68 AG271014_69 AG271014_70 AG261114_32 AG261114_33 AG261114_34 AG261114_35 AG261114_67 AG261114_68 AG261114_69 AG261114_70 AG140814_12 AG140814_13 AG140814_14 AG140814_15 AG130814_47 AG130814_48 AG130814_49 AG130814_50 AG251114_12 AG251114_13 AG251114_14 AG251114_15 AG251114_47 AG251114_48 AG251114_49 AG251114_50 AG271014_12 AG271014_13 AG271014_14 AG271014_15 AG271014_47 AG271014_48 AG271014_49 AG271014_50 AG261114_12 AG261114_13 AG261114_14 AG261114_15 AG261114_47 AG261114_48 AG261114_49 AG261114_50 AG140814_17 AG140814_18 AG140814_19 AG140814_20 AG130814_52 AG130814_53 AG130814_54 AG130814_55 AG251114_17 AG251114_18 AG251114_19 AG251114_20 AG251114_52 AG251114_53 AG251114_54 AG251114_55 AG271014_17 AG271014_18 AG271014_19 AG271014_20 AG271014_52 AG271014_53 AG271014_54 AG271014_55 AG261114_17 AG261114_18 AG261114_19 AG261114_20 AG261114_52 AG261114_53 AG261114_54 AG261114_55 AG140814_22 AG140814_23 AG140814_24 AG140814_25 AG130814_57 AG130814_58 AG130814_59 AG130814_60 AG251114_22 AG251114_23 AG251114_24 AG251114_25 AG251114_57 AG251114_58 AG251114_59 AG251114_60 AG271014_22 AG271014_23 AG271014_24 AG271014_25 AG271014_57 AG271014_58 AG271014_59 AG271014_60 AG261114_22 AG261114_23 AG261114_24 AG261114_25 AG261114_57 AG261114_58 AG261114_59 AG261114_60 AG140814_10 AG140814_7 AG140814_8 AG140814_9 AG130814_42 AG130814_43 AG130814_44 AG130814_45 AG251114_10 AG251114_7 AG251114_8 AG251114_9 AG251114_42 AG251114_43 AG251114_44 AG251114_45 AG271014_10 AG271014_7 AG271014_8 AG271014_9 AG271014_42 AG271014_43 AG271014_44 AG271014_45 AG261114_10 AG261114_7 AG261114_8 AG261114_9 AG261114_42 AG261114_43 AG261114_44 AG261114_45 Factors Time (mins):12 | Nucleotide:ATP | Yeast Matrix:No Time (mins):12 | Nucleotide:ATP | Yeast Matrix:No Time (mins):12 | Nucleotide:ATP | Yeast Matrix:No Time (mins):12 | Nucleotide:ATP | Yeast Matrix:No Time (mins):12 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):12 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):12 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):12 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):12 | Nucleotide:CTP | Yeast Matrix:No Time (mins):12 | Nucleotide:CTP | Yeast Matrix:No Time (mins):12 | Nucleotide:CTP | Yeast Matrix:No Time (mins):12 | Nucleotide:CTP | Yeast Matrix:No Time (mins):12 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):12 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):12 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):12 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):12 | Nucleotide:GTP | Yeast Matrix:No Time (mins):12 | Nucleotide:GTP | Yeast Matrix:No Time (mins):12 | Nucleotide:GTP | Yeast Matrix:No Time (mins):12 | Nucleotide:GTP | Yeast Matrix:No Time (mins):12 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):12 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):12 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):12 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):12 | Nucleotide:UTP | Yeast Matrix:No Time (mins):12 | Nucleotide:UTP | Yeast Matrix:No Time (mins):12 | Nucleotide:UTP | Yeast Matrix:No Time (mins):12 | Nucleotide:UTP | Yeast Matrix:No Time (mins):12 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):12 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):12 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):12 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):15 | Nucleotide:ATP | Yeast Matrix:No Time (mins):15 | Nucleotide:ATP | Yeast Matrix:No Time (mins):15 | Nucleotide:ATP | Yeast Matrix:No Time (mins):15 | Nucleotide:ATP | Yeast Matrix:No Time (mins):15 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):15 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):15 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):15 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):15 | Nucleotide:CTP | Yeast Matrix:No Time (mins):15 | Nucleotide:CTP | Yeast Matrix:No Time (mins):15 | Nucleotide:CTP | Yeast Matrix:No Time (mins):15 | Nucleotide:CTP | Yeast Matrix:No Time (mins):15 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):15 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):15 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):15 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):15 | Nucleotide:GTP | Yeast Matrix:No Time (mins):15 | Nucleotide:GTP | Yeast Matrix:No Time (mins):15 | Nucleotide:GTP | Yeast Matrix:No Time (mins):15 | Nucleotide:GTP | Yeast Matrix:No Time (mins):15 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):15 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):15 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):15 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):15 | Nucleotide:UTP | Yeast Matrix:No Time (mins):15 | Nucleotide:UTP | Yeast Matrix:No Time (mins):15 | Nucleotide:UTP | Yeast Matrix:No Time (mins):15 | Nucleotide:UTP | Yeast Matrix:No Time (mins):15 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):15 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):15 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):15 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):3 | Nucleotide:ATP | Yeast Matrix:No Time (mins):3 | Nucleotide:ATP | Yeast Matrix:No Time (mins):3 | Nucleotide:ATP | Yeast Matrix:No Time (mins):3 | Nucleotide:ATP | Yeast Matrix:No Time (mins):3 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):3 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):3 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):3 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):3 | Nucleotide:CTP | Yeast Matrix:No Time (mins):3 | Nucleotide:CTP | Yeast Matrix:No Time (mins):3 | Nucleotide:CTP | Yeast Matrix:No Time (mins):3 | Nucleotide:CTP | Yeast Matrix:No Time (mins):3 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):3 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):3 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):3 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):3 | Nucleotide:GTP | Yeast Matrix:No Time (mins):3 | Nucleotide:GTP | Yeast Matrix:No Time (mins):3 | Nucleotide:GTP | Yeast Matrix:No Time (mins):3 | Nucleotide:GTP | Yeast Matrix:No Time (mins):3 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):3 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):3 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):3 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):3 | Nucleotide:UTP | Yeast Matrix:No Time (mins):3 | Nucleotide:UTP | Yeast Matrix:No Time (mins):3 | Nucleotide:UTP | Yeast Matrix:No Time (mins):3 | Nucleotide:UTP | Yeast Matrix:No Time (mins):3 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):3 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):3 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):3 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):6 | Nucleotide:ATP | Yeast Matrix:No Time (mins):6 | Nucleotide:ATP | Yeast Matrix:No Time (mins):6 | Nucleotide:ATP | Yeast Matrix:No Time (mins):6 | Nucleotide:ATP | Yeast Matrix:No Time (mins):6 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):6 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):6 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):6 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):6 | Nucleotide:CTP | Yeast Matrix:No Time (mins):6 | Nucleotide:CTP | Yeast Matrix:No Time (mins):6 | Nucleotide:CTP | Yeast Matrix:No Time (mins):6 | Nucleotide:CTP | Yeast Matrix:No Time (mins):6 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):6 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):6 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):6 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):6 | Nucleotide:GTP | Yeast Matrix:No Time (mins):6 | Nucleotide:GTP | Yeast Matrix:No Time (mins):6 | Nucleotide:GTP | Yeast Matrix:No Time (mins):6 | Nucleotide:GTP | Yeast Matrix:No Time (mins):6 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):6 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):6 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):6 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):6 | Nucleotide:UTP | Yeast Matrix:No Time (mins):6 | Nucleotide:UTP | Yeast Matrix:No Time (mins):6 | Nucleotide:UTP | Yeast Matrix:No Time (mins):6 | Nucleotide:UTP | Yeast Matrix:No Time (mins):6 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):6 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):6 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):6 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):9 | Nucleotide:ATP | Yeast Matrix:No Time (mins):9 | Nucleotide:ATP | Yeast Matrix:No Time (mins):9 | Nucleotide:ATP | Yeast Matrix:No Time (mins):9 | Nucleotide:ATP | Yeast Matrix:No Time (mins):9 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):9 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):9 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):9 | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):9 | Nucleotide:CTP | Yeast Matrix:No Time (mins):9 | Nucleotide:CTP | Yeast Matrix:No Time (mins):9 | Nucleotide:CTP | Yeast Matrix:No Time (mins):9 | Nucleotide:CTP | Yeast Matrix:No Time (mins):9 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):9 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):9 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):9 | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):9 | Nucleotide:GTP | Yeast Matrix:No Time (mins):9 | Nucleotide:GTP | Yeast Matrix:No Time (mins):9 | Nucleotide:GTP | Yeast Matrix:No Time (mins):9 | Nucleotide:GTP | Yeast Matrix:No Time (mins):9 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):9 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):9 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):9 | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):9 | Nucleotide:UTP | Yeast Matrix:No Time (mins):9 | Nucleotide:UTP | Yeast Matrix:No Time (mins):9 | Nucleotide:UTP | Yeast Matrix:No Time (mins):9 | Nucleotide:UTP | Yeast Matrix:No Time (mins):9 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):9 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):9 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):9 | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):- | Nucleotide:ATP | Yeast Matrix:No Time (mins):- | Nucleotide:ATP | Yeast Matrix:No Time (mins):- | Nucleotide:ATP | Yeast Matrix:No Time (mins):- | Nucleotide:ATP | Yeast Matrix:No Time (mins):- | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):- | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):- | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):- | Nucleotide:ATP | Yeast Matrix:Yes Time (mins):- | Nucleotide:CTP | Yeast Matrix:No Time (mins):- | Nucleotide:CTP | Yeast Matrix:No Time (mins):- | Nucleotide:CTP | Yeast Matrix:No Time (mins):- | Nucleotide:CTP | Yeast Matrix:No Time (mins):- | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):- | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):- | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):- | Nucleotide:CTP | Yeast Matrix:Yes Time (mins):- | Nucleotide:GTP | Yeast Matrix:No Time (mins):- | Nucleotide:GTP | Yeast Matrix:No Time (mins):- | Nucleotide:GTP | Yeast Matrix:No Time (mins):- | Nucleotide:GTP | Yeast Matrix:No Time (mins):- | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):- | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):- | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):- | Nucleotide:GTP | Yeast Matrix:Yes Time (mins):- | Nucleotide:UTP | Yeast Matrix:No Time (mins):- | Nucleotide:UTP | Yeast Matrix:No Time (mins):- | Nucleotide:UTP | Yeast Matrix:No Time (mins):- | Nucleotide:UTP | Yeast Matrix:No Time (mins):- | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):- | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):- | Nucleotide:UTP | Yeast Matrix:Yes Time (mins):- | Nucleotide:UTP | Yeast Matrix:Yes ADP 16976.6500 17265.0100 7687.4870 13986.2800 3767.6820 4770.5500 4979.7920 4622.5410 9938.9050 9784.5120 9563.7830 11305.9000 3704.3530 5038.3460 4475.0530 4439.2010 11133.8500 14452.5300 12607.2800 11989.3800 6516.8590 6133.9360 4061.3900 4499.0640 7049.8020 11624.3500 13127.9000 12813.1300 4102.4770 4933.5300 6390.1790 6139.7180 13575.0400 15260.1900 14568.6200 16549.6300 5311.5970 8767.1540 5664.9510 4059.0720 11319.7800 10167.1000 8707.2230 8703.4430 8369.3090 6066.7400 6724.8810 8609.6190 ADP 13C15N IS 228729.7000 202852.9000 99332.8800 157685.5000 102515.7000 155134.9000 131404.8000 136357.7000 117484.2000 108214.4000 110179.5000 156197.0000 111267.7000 146312.0000 160420.6000 138744.8000 192503.8000 220692.4000 193293.4000 196820.2000 234272.8000 218850.6000 118626.6000 140568.2000 117801.4000 160558.6000 203796.1000 189474.8000 134628.3000 153210.9000 219057.5000 220041.0000 198768.1000 211252.2000 194845.4000 205154.6000 170243.8000 246811.8000 174291.1000 131180.4000 220025.8000 225353.1000 203372.4000 176498.3000 241158.2000 174833.3000 208635.6000 271247.2000 AMP 883.6780 896.2950 521.3880 644.5860 2090.5150 3085.7950 2689.2980 2792.8860 603.4400 578.3190 526.3050 696.4530 2132.7730 2966.7180 3243.1120 1066.0160 800.5180 857.2370 906.1110 796.4490 3504.3640 3602.2000 2107.1880 2410.0870 493.9490 792.1030 788.8640 791.5440 2267.8580 2649.6940 3576.0400 3604.1490 773.7490 947.1220 840.7870 935.6590 2749.4180 3985.2730 3239.5360 2635.1060 961.1030 721.6770 753.6500 719.3570 3825.4590 3007.9430 3512.8320 4183.8540 AMP 13C15N IS 25720.8400 23110.7100 13999.5000 17728.0900 14980.1100 20981.9400 19263.4500 19580.5700 16209.3000 14205.0000 13639.9700 19031.9700 15220.6000 19682.6600 20218.0200 20014.7200 25514.4000 26879.2000 27042.6300 25332.6700 26459.1400 26876.7800 14933.8000 18173.5500 14942.4200 21398.5700 26750.7100 22950.6200 16281.8500 18204.3400 26008.0600 27335.6300 23079.4400 24603.9600 23174.6800 25900.2100 20747.5400 26481.5800 23144.9400 18918.8000 31880.3400 30642.8800 26912.9000 24416.1800 26755.9800 22075.4900 25496.7300 30475.9600 ATP 140608.3000 125829.7000 42552.6500 98430.4100 59679.7900 78497.9400 91811.5800 79958.4600 53558.7000 51296.5400 57999.2700 90438.8500 62342.7900 80740.1600 84746.2200 73698.5900 115457.5000 143688.8000 134232.0000 129174.2000 82860.6500 76605.3600 53246.1100 58407.4100 52267.8800 90263.0500 141499.8000 107219.3000 61688.0700 88128.6900 98305.0300 97412.5300 110672.5000 130745.5000 136330.3000 129845.9000 81830.2200 135865.4000 90543.1300 80942.3800 143016.0000 126541.7000 150012.1000 95540.9300 99386.7800 75237.7300 100970.0000 111492.8000 ATP 13C15N IS 92158.4500 71731.2300 26842.6400 54255.0700 41971.7100 56721.7800 54805.2600 54600.7700 32979.7900 31629.4400 34572.3600 52401.5200 44307.8000 57530.2300 60470.9800 52099.6900 70847.9600 81973.6000 73933.4100 75721.8400 59938.8700 53253.0700 34355.4800 39085.6400 34892.0800 53903.2700 85833.2100 66493.5800 41361.0500 53721.0500 66025.3900 67107.7600 70423.0700 75816.1500 82263.2700 71729.8200 56670.9300 86583.4900 64516.4500 55769.2100 86022.8700 79486.1900 83842.9500 59482.2000 62762.4300 47328.2100 63904.7500 70540.7400 CDP 6791.1850 5291.4160 4879.0780 6006.3490 1939.8020 1928.7070 3767.0590 2216.9860 7321.9480 5732.3170 7308.5990 4778.1540 2754.2740 2464.2830 2381.6040 2731.4600 5430.0310 5197.2700 6387.3430 4964.3730 2127.7890 2418.4680 2640.4400 2711.7300 7060.8980 4797.0570 8224.5890 4023.1860 2251.3200 2467.0590 2902.5260 2610.2160 3446.0920 4284.7810 5309.9730 4868.9590 2339.5980 3918.0050 5193.7760 2771.1810 7906.5690 7534.8810 5701.4510 6706.1600 6096.4380 3928.5090 2080.6620 1831.3790 CDP 13C15N IS 73542.2900 62014.9500 58328.9200 72615.0900 29262.6600 31145.6500 64360.9300 35549.7400 70719.2100 58256.2100 84347.3500 56968.3400 37812.1400 32532.6500 33391.2500 38013.3800 80267.4100 68372.2900 84041.3800 67761.3100 36967.8700 40231.5900 41281.4300 42921.0500 81265.6100 61852.7100 108079.6000 55567.5100 40336.2400 38530.1400 41554.3100 35582.8100 45595.5700 56477.8900 66771.7300 63307.9000 34671.9600 61820.4000 189827.0000 41001.7100 135782.7000 105587.0000 85551.8400 107061.7000 264991.6000 71101.7600 33231.4000 30743.8200 CMP 253.5520 228.0160 173.7310 218.1870 58.1770 77.3710 110.6280 72.8060 291.3530 221.1280 264.8430 147.9200 83.6500 45.0270 77.9700 43.3970 184.7650 169.7210 201.4470 178.5400 59.7910 28.0820 69.4870 68.8610 270.8550 186.5730 263.8790 165.0000 62.4210 55.1530 48.0630 67.2670 151.5840 113.1260 169.7170 217.5170 70.1240 80.9260 61.5860 49.6810 215.6030 304.3680 216.7580 247.9230 47.2740 55.8960 63.2430 44.8620 CMP 13C15N IS 12897.2200 10425.2600 9617.2700 11705.6500 25779.6900 23259.1600 38783.4800 26652.7100 11498.9800 9554.4510 12766.6900 8491.8170 28748.1300 25394.6600 25442.5900 26951.4300 16485.1100 14582.2900 16801.4600 13833.1400 29530.2400 28628.2900 30062.4400 30773.4700 14909.3300 10646.8500 20166.7200 10345.1700 28304.8800 25753.4600 29978.5900 25477.3600 8378.2980 10281.2100 11684.7800 11491.6500 25538.8700 32785.1600 41951.3500 26546.9400 22025.7000 21200.8400 16977.9800 21207.7300 63360.1400 42907.6100 30120.1000 26216.9600 CTP 51240.1300 40506.6600 35989.3900 43246.3600 11055.4800 14994.9500 28533.0600 13975.3100 51410.9000 36249.8300 51064.1600 31931.7400 18233.3800 16980.0200 16095.9000 18562.0300 49889.9500 42403.2600 53519.4900 38555.6300 12953.2400 14444.2900 16400.6700 17140.3500 58041.4300 39968.9200 71896.1600 35244.7000 15694.2000 16353.7400 20785.3300 17444.9200 32079.7600 37481.4000 43253.6000 37957.5800 14270.6700 37733.4300 42119.8400 20185.7300 88931.6800 62796.4100 44331.5600 55641.0300 56459.1300 29198.0200 10398.1100 9685.4170 CTP 13C15N IS 55286.5400 47086.1600 44673.5900 54294.8800 11715.2800 16496.5700 30715.6000 14678.3600 53692.7000 41502.2100 63021.5900 40203.3900 18760.0600 16412.2700 15809.3300 18420.5300 56192.4000 45115.1400 61597.9400 46144.5500 14647.9200 15658.1400 15886.4600 17263.4300 61044.9900 43820.1400 82626.3000 41263.3000 18956.6300 16632.1500 19066.3800 15739.0600 35349.8800 42238.0800 52646.6300 47090.7200 14898.1100 39893.5900 42432.9000 20148.0900 100034.9000 68042.7800 53428.8600 69968.7200 65336.2400 28719.4300 10953.0700 10172.1100 GDP 28801.0300 28838.0200 29485.9700 30398.1900 26354.4000 26169.5600 29035.0300 30110.7000 24488.9200 32031.8000 28296.3100 30012.6100 28671.2200 28636.8500 27214.5800 27746.4600 25502.5800 29874.1000 26159.3600 25039.8000 24060.8400 28265.2600 27842.1300 23888.7800 25681.9700 28636.7000 27888.2300 30778.5000 25236.7400 24571.3300 26351.4400 25272.7600 27817.3600 28916.8600 32012.3100 29055.7800 23296.8100 25869.6800 24823.7000 23208.8700 25994.2900 28354.6900 30065.0900 33617.6300 22704.6600 24283.4400 25028.8700 26487.9900 GDP 13C15N IS 216511.3000 198825.8000 188281.7000 180678.6000 230302.5000 228593.4000 222967.3000 232446.6000 165056.8000 190999.8000 180560.1000 170389.5000 228039.8000 229791.3000 237654.4000 245753.8000 218471.0000 250443.0000 251150.1000 240051.8000 232720.0000 249707.8000 254726.6000 208953.5000 235788.9000 234861.5000 241728.9000 267119.4000 221421.9000 235404.0000 223870.3000 228721.6000 237784.6000 217217.9000 236921.7000 252499.4000 225116.7000 229324.5000 208402.5000 216527.1000 248465.2000 286690.9000 296284.5000 275769.1000 234207.6000 206901.1000 229783.8000 248337.6000 GMP 1056.6380 992.3500 957.0760 1079.4080 922.9830 863.9940 1022.8580 1011.0600 792.3310 1097.1340 1045.5940 937.8700 1150.0280 921.8310 922.3590 903.0540 757.8150 856.5980 817.5940 909.8900 589.3750 810.6910 722.8720 642.8570 874.6400 834.5660 900.0010 1008.5550 759.2740 944.2110 824.1920 797.3860 760.4170 826.8100 992.1250 890.0990 802.4380 875.7010 850.1680 830.8330 906.8540 1078.2540 1018.1140 919.3900 657.6340 772.5020 752.8460 780.6490 GMP 13C15N IS 41674.1000 37176.8400 35376.4400 35049.9300 39524.2500 36402.3000 36693.7300 38932.5100 30834.2100 35809.9800 33060.8900 34112.1700 40200.1700 38262.6300 34517.0500 40489.0500 43296.6300 46389.3900 43361.0400 53758.2600 42317.1300 42489.6300 43198.7700 35454.9800 46281.6600 44484.8400 44931.3900 53321.8900 39221.9600 42590.3900 42701.1600 42023.2600 44189.7400 42681.7700 43901.2500 44544.9100 39397.9500 43600.6100 40070.9400 39663.6100 52254.5300 60535.4500 53129.4800 52438.3100 43785.9700 40240.5900 44217.2800 44106.7600 GTP 226839.9000 235907.3000 235234.5000 253528.3000 250256.4000 257756.3000 306978.6000 330783.2000 208934.3000 229520.7000 215358.1000 227180.7000 304706.4000 279382.6000 263534.6000 277780.2000 206707.5000 225123.2000 257675.3000 228033.1000 211187.8000 266483.6000 274313.4000 241531.7000 225531.7000 275893.6000 276787.0000 287636.0000 256541.8000 292264.5000 276875.1000 285347.4000 210586.2000 227869.5000 262957.7000 255696.1000 218527.1000 264885.2000 244611.7000 234376.2000 283576.6000 235510.8000 289132.9000 292767.2000 194223.8000 212679.6000 235469.6000 240888.6000 GTP 13C15N IS 191200.8000 185808.1000 174760.3000 173213.1000 189076.3000 191448.8000 202936.1000 219201.3000 174548.5000 169780.8000 157137.5000 165098.0000 215819.2000 199430.8000 196794.2000 206231.4000 155170.2000 174167.3000 194611.1000 190993.5000 167646.7000 184178.2000 202769.7000 169720.6000 193961.4000 206469.1000 205974.3000 228016.8000 183127.7000 223804.0000 198610.7000 204926.8000 182182.9000 185788.4000 210906.4000 220681.9000 183881.3000 198976.9000 178439.6000 183553.9000 195497.5000 195925.7000 214942.0000 200148.3000 155788.0000 139343.0000 165254.0000 175658.7000 UDP 1646.4880 1801.2510 2105.5720 2036.6810 2338.3190 1280.1600 1306.7290 1058.7940 2095.5260 2187.1730 2191.1990 2134.5840 1716.0070 1078.8050 2149.3860 1029.1760 3164.1410 3253.0400 3579.3290 2651.1630 1802.6160 1075.6580 2199.0460 1167.5430 3628.2880 2938.9390 2893.6220 3050.1770 1624.0810 1036.6380 2023.8060 1337.5630 2133.2450 2142.7820 2193.1830 1982.6380 1567.5500 1081.7900 1009.1680 870.7120 1477.2940 1863.1070 2364.3260 1816.1440 2278.7540 1535.7080 2193.8850 1050.7890 UDP 13C15N IS 25684.1000 32527.1400 40417.1200 35766.4300 48652.7900 30814.4500 30721.3900 23810.9000 32607.0700 37343.2700 37402.1100 34847.4800 42714.8600 25398.4600 48400.7500 21229.8100 71200.9800 65598.9000 71937.4700 54296.1200 47332.2800 28762.0200 49266.0000 27216.3900 53741.5500 49793.9600 46256.1000 56754.7300 40474.5500 29101.6400 43232.4000 27936.0700 39045.6600 42225.5400 42546.4700 38711.6800 36523.5500 25208.7300 20924.9200 20264.3800 27787.3000 39233.7700 59860.9600 45590.1400 49993.0000 34852.0000 57473.3800 24878.5500 UMP 31.6220 24.0010 27.2270 42.1810 88.0480 40.5520 30.6700 44.2070 50.6730 35.4010 29.1010 51.7790 56.3260 32.5980 68.3970 51.2640 28.4840 47.6290 39.6770 46.0490 47.6810 34.8800 53.3130 29.4330 41.7110 55.3290 21.2730 39.6000 46.9630 18.9260 60.0800 38.1100 31.9650 41.9090 28.5980 43.2720 45.7600 46.4380 35.2070 55.2310 36.3780 39.8820 35.8390 114.4790 68.6160 42.3040 66.6640 34.6860 UMP 13C15N IS 9586.2970 10042.0000 13101.2500 10438.3000 22507.6600 14303.7500 12982.3800 11603.6500 10496.6800 11562.8800 10407.9400 11941.5800 20633.9500 12509.0700 19227.4200 19533.3100 22241.5400 19269.0300 22047.8200 16835.4600 20476.3800 13099.3700 22099.5100 13659.3000 16598.0500 16570.3500 13905.3400 12262.1700 18727.3300 13748.4300 19358.2900 13107.5900 13120.2700 13710.8100 14520.6000 13217.3700 16539.6300 14577.6600 12085.3500 11371.2500 9035.2550 13339.9400 19621.1900 13433.9100 20692.4500 14587.6700 24235.2700 12761.9900 UTP 8409.0270 10414.5000 12443.7000 11515.0000 14833.6100 7226.1920 7174.3350 5527.8610 10753.5800 12604.4300 10923.1200 11131.1200 9812.8030 6664.8170 11296.1300 5111.2680 32706.7800 29909.4600 30885.9100 22280.0700 12620.2500 6498.0050 13078.8300 7018.6620 27329.4700 24723.0400 22308.1200 17668.2300 9810.0930 6629.2850 11782.6600 7931.4350 14939.8400 13016.1700 13045.9700 12344.8600 7666.3660 5630.1150 5330.8240 4502.6020 13566.6200 16971.4700 21426.9700 19952.2200 13657.1100 8786.2330 14084.2400 5680.7570 UTP 13C15N IS 8058.3700 10852.3100 13974.0400 12423.6600 16203.0900 8227.3590 8119.8740 6142.6660 11234.7100 13617.6700 11457.9200 12218.0100 11705.3800 8052.6330 13255.2400 5717.0350 35617.2900 30604.4800 32302.9500 25723.9300 14514.4400 7254.3360 13152.7500 6944.4200 21885.8300 22524.6600 20089.5800 16047.8000 11580.4800 7681.2840 11182.3600 7497.8000 13647.0200 13743.8700 14278.4400 12971.8300 8188.7500 5995.2070 5853.5390 4768.8700 13487.0800 17330.4200 25202.4300 21055.2300 13273.9600 8514.0420 15436.8100 6124.0860 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant ADP 6022 C00008 426.022 ADP 13C15N IS AMP 6083 C00020 346.055 AMP 13C15N IS ATP 5957 C00002 505.988 ATP 13C15N IS CDP 6132 C00112 402.011 CDP 13C15N IS CMP 6131 C00055 322.045 CMP 13C15N IS CTP 6176 C00063 481.977 CTP 13C15N IS GDP 8977 C00035 442.017 GDP 13C15N IS GMP 6804 C00144 362.051 GMP 13C15N IS GTP 6830 C00044 521.983 GTP 13C15N IS UDP 6031 C00015 402.995 UDP 13C15N IS UMP 6030 C00105 323.029 UMP 13C15N IS UTP 6133 C00075 482.961 UTP 13C15N IS METABOLITES_END #END