#METABOLOMICS WORKBENCH KunZuo_20190410_234734_mwtab.txt DATATRACK_ID:1700 STUDY_ID:ST001168 ANALYSIS_ID:AN001932 PROJECT_ID:PR000780
VERSION             	1
CREATED_ON             	April 10, 2019, 11:57 pm
#PROJECT
PR:PROJECT_TITLE                 	Alterations in fecal metabolic patterns are associated with atrial fibrillation
PR:PROJECT_SUMMARY               	Little evidence has been reported in characterizing the fecal alterations in
PR:PROJECT_SUMMARY               	metabolic patterns in atrial fibrillation (AF). We include the result of the
PR:PROJECT_SUMMARY               	global alterations that occur in the intestinal microbiota in a cohort of AF
PR:PROJECT_SUMMARY               	patients and matched controls based on a strategy of metabolomic analyses. Our
PR:PROJECT_SUMMARY               	findings characterize the disordered microbial metabolite profiles in AF.
PR:INSTITUTE                     	Beijing Chaoyang Hospital
PR:LAST_NAME                     	Zuo
PR:FIRST_NAME                    	Kun
PR:ADDRESS                       	8th Gongtinanlu Rd,, Chaoyang District, Beijing, Beijing, 10020, China
PR:EMAIL                         	zuokun699@163.com
PR:PHONE                         	10-86-15210511744
#STUDY
ST:STUDY_TITLE                   	Alterations in fecal metabolic patterns are associated with atrial fibrillation
ST:STUDY_SUMMARY                 	Little evidence has been reported in characterizing the fecal alterations in
ST:STUDY_SUMMARY                 	metabolic patterns in atrial fibrillation (AF). We include the result of the
ST:STUDY_SUMMARY                 	global alterations that occur in the intestinal microbiota in a cohort of AF
ST:STUDY_SUMMARY                 	patients and matched controls based on a strategy of metabolomic analyses. Our
ST:STUDY_SUMMARY                 	findings characterize the disordered microbial metabolite profiles in AF.
ST:INSTITUTE                     	Beijing Chaoyang Hospital
ST:LAST_NAME                     	Kun
ST:FIRST_NAME                    	Zuo
ST:ADDRESS                       	8th Gongtinanlu Rd, Chaoyang District, Beijing, China, 100020
ST:EMAIL                         	zuokun699@163.com
ST:PHONE                         	86-10-15210511744
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	af9	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af10	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af11	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af12	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af13	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af14	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af15	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af16	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af17	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af18	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af19	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af20	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af21	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af22	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af23	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af24	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af25	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af26	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af27	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af28	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af29	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af30	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af31	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af32	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af33	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af34	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af35	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af36	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af37	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af38	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af39	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af40	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af41	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af42	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af43	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af44	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af45	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af46	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af47	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af48	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af49	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	af50	Group:A	
SUBJECT_SAMPLE_FACTORS           	-	FA6	Group:C	
SUBJECT_SAMPLE_FACTORS           	-	FA7	Group:C	
SUBJECT_SAMPLE_FACTORS           	-	FA9	Group:C	
SUBJECT_SAMPLE_FACTORS           	-	FA13	Group:C	
SUBJECT_SAMPLE_FACTORS           	-	FA4	Group:C	
SUBJECT_SAMPLE_FACTORS           	-	FA2	Group:C	
SUBJECT_SAMPLE_FACTORS           	-	FA12	Group:C	
SUBJECT_SAMPLE_FACTORS           	-	FA3	Group:C	
SUBJECT_SAMPLE_FACTORS           	-	FC10	Group:C	
SUBJECT_SAMPLE_FACTORS           	-	FC12	Group:C	
SUBJECT_SAMPLE_FACTORS           	-	FC2	Group:C	
SUBJECT_SAMPLE_FACTORS           	-	FC17	Group:C	
SUBJECT_SAMPLE_FACTORS           	-	FC29	Group:C	
SUBJECT_SAMPLE_FACTORS           	-	FC14	Group:C	
SUBJECT_SAMPLE_FACTORS           	-	FC16	Group:C	
SUBJECT_SAMPLE_FACTORS           	-	FC13	Group:C	
SUBJECT_SAMPLE_FACTORS           	-	FC9	Group:C	
#COLLECTION
CO:COLLECTION_SUMMARY            	Fecal samples were collected from each participant, immediately frozen at −20
CO:COLLECTION_SUMMARY            	°C, transported on ice to the laboratory and then stored at −80 °C.
CO:SAMPLE_TYPE                   	Feces
#TREATMENT
TR:TREATMENT_SUMMARY             	To explore how the host metabolic pattern alterations were impacted by the gut
TR:TREATMENT_SUMMARY             	microbiota dysbiosis in AF patients, serum samples were collected and analyzed
TR:TREATMENT_SUMMARY             	by high-throughput liquid chromatography-mass spectrometry (LC/MS).
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	50 mg fecal samples were pipetted into centrifuge tubes (1.5 mL) in preparation
SP:SAMPLEPREP_SUMMARY            	for extraction. The protein was precipitated with 800 μL of methanol and 10 μL
SP:SAMPLEPREP_SUMMARY            	of internal standard (2.9 mg/mL, DL-o-Chlorophenylalanine) was added. The
SP:SAMPLEPREP_SUMMARY            	samples were ground at 65 KHz for 90 s and centrifuged at 12000 rpm for 15 min
SP:SAMPLEPREP_SUMMARY            	at 4 °C. 200 μL of the supernatant was transferred into a vial for further
SP:SAMPLEPREP_SUMMARY            	analysis.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
MS:INSTRUMENT_NAME               	Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap
CH:COLUMN_NAME                   	Thermo Hypergod C18 (100 × 4.6 mm 3 µm)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	All metabolomic data were prepared for feature extraction and preprocessed with
MS:MS_COMMENTS                   	Compound Discoverer 2.0 software (Thermo). Using SIMCA-P software (Umetrics AB,
MS:MS_COMMENTS                   	Umea, Sweden), a multivariate Analysis (MVA) was performed. The exact molecular
MS:MS_COMMENTS                   	mass, ppm and ms/ms value of these compounds was used to identify the
MS:MS_COMMENTS                   	metabolites related to the featured peak in the Metlin database
MS:MS_COMMENTS                   	(http://metlin.scripps.edu). The score value indicated the matching rate was
MS:MS_COMMENTS                   	calculated by Compound Discoverer 2.0 software (Thermo) with max of 100.
MS:MS_RESULTS_FILE               	ST001168_AN001932_Results.txt	UNITS:feature area	Has m/z:Neutral masses	Has RT:Yes	RT units:Minutes
#END