#METABOLOMICS WORKBENCH kameiyasutomi_20190422_012754 DATATRACK_ID:1708 STUDY_ID:ST001178 ANALYSIS_ID:AN001955 PROJECT_ID:PR000790
VERSION             	1
CREATED_ON             	May 1, 2019, 2:29 pm
#PROJECT
PR:PROJECT_TITLE                 	Metabolomic analysis of C2C12 myoblasts induced by the transcriptional factor
PR:PROJECT_TITLE                 	FOXO1
PR:PROJECT_SUMMARY               	To determine the metabolic changes occurring due to the activation of
PR:PROJECT_SUMMARY               	transcriptional factor FOXO1, we analyzed the metabolic profile of C2C12
PR:PROJECT_SUMMARY               	myoblasts expressing FOXO1-estrogen receptor fusion protein using CE-TOFMS.
PR:INSTITUTE                     	Kyoto Prefectural University
PR:LAST_NAME                     	Kamei
PR:FIRST_NAME                    	Yasutomi
PR:ADDRESS                       	1-5 Hangi-cho, Shimogamo, Sakyo-ku Kyoto, Kyoto, Kyoto, 606-8522, Japan
PR:EMAIL                         	cnqwb974@yahoo.co.jp
PR:PHONE                         	+81-75-703-5661
#STUDY
ST:STUDY_TITLE                   	Metabolomic analysis of C2C12 myoblasts induced by the transcriptional factor
ST:STUDY_TITLE                   	FOXO1
ST:STUDY_SUMMARY                 	The transcriptional factor FOXO1 is considered to play roles in the regulation
ST:STUDY_SUMMARY                 	of energy metabolism in various tissues. To determine the metabolic changes
ST:STUDY_SUMMARY                 	occurring due to the activation of FOXO1, we analyzed the metabolic profile of
ST:STUDY_SUMMARY                 	C2C12 myoblasts expressing FOXO1-estrogen receptor fusion protein using
ST:STUDY_SUMMARY                 	CE-TOFMS. In the FOXO1-activated cells, the metabolite levels during glycolysis
ST:STUDY_SUMMARY                 	were higher. In addition, the gene expression of pyruvate dehydrogenase kinase,
ST:STUDY_SUMMARY                 	an enzyme that inhibits glucose utilization, increased. In the FOXO1-activated
ST:STUDY_SUMMARY                 	cells, the metabolite levels of numerous amino acids decreased, with increased
ST:STUDY_SUMMARY                 	gene expression of branched chain amino acid metabolism enzymes. Our results
ST:STUDY_SUMMARY                 	suggest that FOXO1 suppresses glucose utilization and promotes the use of
ST:STUDY_SUMMARY                 	proteins/amino acids as energy sources in muscle cells, potentially during
ST:STUDY_SUMMARY                 	starvation.
ST:INSTITUTE                     	Kyoto Prefectural University
ST:LAST_NAME                     	Kamei
ST:FIRST_NAME                    	Yasutomi
ST:ADDRESS                       	1-5 Hangi-cho, Shimogamo, Sakyo-ku Kyoto, Kyoto, Kyoto, 606-8522, Japan
ST:EMAIL                         	cnqwb974@yahoo.co.jp
ST:PHONE                         	+81-75-703-5661
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	Control 1	treatment:control	Hours=24; tamoxifen(µM）=-; Cells(×106cells)=3.9
SUBJECT_SAMPLE_FACTORS           	-	Control 2	treatment:control	Hours=24; tamoxifen(µM）=-; Cells(×106cells)=3.9
SUBJECT_SAMPLE_FACTORS           	-	Control 3	treatment:control	Hours=24; tamoxifen(µM）=-; Cells(×106cells)=3.9
SUBJECT_SAMPLE_FACTORS           	-	FOXO1-ER1	treatment:tamoxifen	Hours=24; tamoxifen(µM）=2; Cells(×106cells)=3.7
SUBJECT_SAMPLE_FACTORS           	-	FOXO1-ER2	treatment:tamoxifen	Hours=24; tamoxifen(µM）=2; Cells(×106cells)=3.7
SUBJECT_SAMPLE_FACTORS           	-	FOXO1-ER3	treatment:tamoxifen	Hours=24; tamoxifen(µM）=2; Cells(×106cells)=3.7
#COLLECTION
CO:COLLECTION_SUMMARY            	Cells were washed with cold PBS and then flash-frozen in liquid N2.
CO:SAMPLE_TYPE                   	Muscle
#TREATMENT
TR:TREATMENT_SUMMARY             	Two days after confluence, cells were treated with tamoxifen for 24 h and used
TR:TREATMENT_SUMMARY             	for the metabolomic analysis.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	The cells treated with vehicle (control, N = 3) or tamoxifen (FOXO1-ER, N = 3)
SP:SAMPLEPREP_SUMMARY            	were used for the metabolomic analysis (Human Metabolome Technologies Inc.,
SP:SAMPLEPREP_SUMMARY            	Tsuruoka, Japan). 3.9 x 106 cells were transferred into 500 μL of methanol
SP:SAMPLEPREP_SUMMARY            	containing 50 mM external standard. After homogenization by BMSM10N21 (BMS,
SP:SAMPLEPREP_SUMMARY            	Tokyo) at 1,500 rpm for 120 s performed 5 times, 500 μL of chloroform and 200
SP:SAMPLEPREP_SUMMARY            	μL of ultrapure water were added to the homogenate. The solution was mixed well
SP:SAMPLEPREP_SUMMARY            	and centrifuged at 2,300 × g for 5 min at 4 °C. The resultant water phase was
SP:SAMPLEPREP_SUMMARY            	ultrafiltered using a Millipore Ultrafree-MC PLHCC HMT Centrifugal Filter
SP:SAMPLEPREP_SUMMARY            	Device, 5 kDa (Millipore, Billerica, MA). The filtrate was dried and then
SP:SAMPLEPREP_SUMMARY            	dissolved in 50 μL of ultrapure water. The samples obtained were then subjected
SP:SAMPLEPREP_SUMMARY            	to capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS)
SP:SAMPLEPREP_SUMMARY            	analysis using the Agilent CE-TOFMS system (Agilent Technologies, Santa Clara,
SP:SAMPLEPREP_SUMMARY            	CA) at 4 °C. The detected peaks were aligned according to their m/z values and
SP:SAMPLEPREP_SUMMARY            	normalized migration times. The peaks were mean-centered and scaled using their
SP:SAMPLEPREP_SUMMARY            	standard deviations on a per peak basis as a pretreatment.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	None (Direct infusion)
CH:INSTRUMENT_NAME               	none
CH:COLUMN_NAME                   	none
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent CE-TOFMS
MS:INSTRUMENT_TYPE               	Other
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	-
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	fold
MS_METABOLITE_DATA_START
Samples	Control 1	Control 2	Control 3	FOXO1-ER1	FOXO1-ER2	FOXO1-ER3
Factors	treatment:control	treatment:control	treatment:control	treatment:tamoxifen	treatment:tamoxifen	treatment:tamoxifen
Pyruvic acid	0.02	0.01	0.01	0.01	0.01	0.01
Lactic acid	1.30	0.58	0.50	1.90	1.80	1.20
3-Hydroxybutyric acid	0.00	0.00		0.00	0.00	0.00
Glyceric acid	0.00	0.00		0.00	0.00	0.00
XA0002	0.02	0.02	0.02	0.03	0.03	0.03
Fumaric acid	0.01	0.01	0.01	0.01	0.01	0.01
2-Oxoisovaleric acid	0.01	0.00	0.00	0.00	0.00	0.00
Hexanoic acid			0.00	0.00		0.00
Succinic acid	0.01	0.01	0.01	0.01	0.01	0.01
Isethionic acid	0.00	0.00	0.00	0.00	0.00	0.00
5-Oxoproline	0.34	0.14	0.13	0.30	0.30	0.23
4-Methyl-2-oxovaleric acid	0.05	0.02	0.02	0.03	0.02	0.01
3-Methyl-2-oxovaleric acid	0.05	0.02	0.02	0.03	0.02	0.01
N-Acetyl-β-alanine	0.00	0.00	0.00	0.00	0.00	0.00
Malic acid	0.08	0.06	0.07	0.11	0.13	0.11
Threonic acid	0.03	0.02	0.03	0.03	0.02	0.03
Ethanolamine phosphate	0.01	0.01	0.01	0.01	0.01	0.01
Octanoic acid			0.00			0.00
XA0004	0.00	0.00	0.00	0.00		0.00
2-Oxoglutaric acid	0.03	0.02	0.03	0.03	0.03	0.04
2-Hydroxyglutaric acid	0.00	0.00	0.00	0.00	0.00	0.00
Pelargonic acid	0.00	0.00	0.00	0.00	0.00	0.00
3-Hydroxy-3-methylglutaric acid	0.00	0.00	0.00	0.00	0.00	0.00
N-Acetylcysteine	0.00			0.00		0.00
Terephthalic acid	0.00	0.00	0.00	0.00	0.00	0.00
XA0012	0.00	0.00	0.00	0.01	0.00	0.00
Phosphoenolpyruvic acid	0.00	0.00	0.00	0.00	0.01	0.01
Uric acid	0.00	0.00	0.00	0.00	0.00	0.00
Glyceraldehyde 3-phosphate	0.00	0.00	0.00	0.01	0.00	0.01
Dihydroxyacetone phosphate	0.01	0.01	0.00	0.06	0.04	0.04
Glycerol 3-phosphate	0.01	0.01	0.01	0.02	0.02	0.02
Decanoic acid	0.00	0.00	0.00	0.00		0.00
cis-Aconitic acid	0.02	0.02	0.02	0.02	0.01	0.01
N-Carbamoylaspartic acid	0.00	0.00	0.00			
O-Phosphoserine	0.00	0.00	0.00	0.00	0.00	0.00
3-Phosphoglyceric acid	0.00	0.00	0.00	0.01	0.01	0.01
2-Phosphoglyceric acid		0.00		0.00	0.00	0.00
N-Acetylglutamine	0.00	0.00	0.00			
N-Acetylglutamic acid	0.00	0.00	0.00	0.00	0.00	0.00
Isocitric acid	0.01	0.01	0.01	0.02	0.01	0.01
Citric acid	0.27	0.23	0.23	0.33	0.24	0.21
Quinic acid			0.00			
Glucuronic acid-1	0.00	0.00	0.00	0.00	0.00	0.00
Galacturonic acid-1	0.00	0.00	0.00	0.00	0.00	0.00
Glucuronic acid-2	0.00	0.00	0.00	0.00	0.00	0.00
Galacturonic acid-2	0.00	0.00	0.00	0.00	0.00	0.00
Gluconic acid	0.04	0.03	0.04	0.09	0.06	0.07
Lauric acid	0.02	0.01	0.02	0.01	0.01	0.01
Phosphocreatine	0.70	0.66	0.72	0.55	0.59	0.56
O-Succinylhomoserine	0.00	0.00	0.00	0.00	0.00	0.00
Pantothenic acid	0.04	0.03	0.03	0.01	0.01	0.01
XA0027	0.00	0.00	0.00	0.00	0.00	0.00
Ribose 5-phosphate	0.00	0.00	0.00	0.01	0.01	0.01
Ribulose 5-phosphate	0.01	0.01	0.01	0.03	0.02	0.02
XA0033	0.17	0.15	0.15	0.13	0.12	0.12
Glucose 1-phosphate	0.03	0.03	0.03	0.06	0.05	0.05
Glucose 6-phosphate	0.01	0.01	0.01	0.03	0.03	0.04
Fructose 6-phosphate	0.00	0.00	0.00	0.01	0.01	0.01
myo-Inositol 1-phosphate	0.01	0.02	0.02	0.02	0.03	0.02
myo-Inositol 3-phosphate	0.01	0.02	0.02	0.02	0.03	0.02
myo-Inositol 2-phosphate	0.00	0.00	0.00	0.00	0.00	0.00
Sulfotyrosine	0.00	0.00	0.00	0.00	0.00	0.00
Sorbitol 6-phosphate	0.00	0.00	0.00	0.00	0.00	0.00
2,3-Diphosphoglyceric acid	0.00	0.00	0.00	0.00	0.00	0.00
6-Phosphogluconic acid	0.00	0.00	0.00	0.00	0.00	0.00
Rhein	0.00	0.00	0.00	0.00	0.00	0.00
Sedoheptulose 7-phosphate	0.01	0.00	0.01	0.02	0.01	0.02
N-Acetylglucosamine 1-phosphate	0.00	0.00	0.00	0.00	0.00	0.00
N-Acetylglucosamine 6-phosphate	0.00	0.00	0.00	0.00	0.00	0.00
N-Acetylneuraminic acid	0.00	0.00	0.00	0.00	0.00	0.00
Ribulose 1,5-diphosphate	0.00	0.00	0.00	0.00	0.00	0.00
3'-CMP	0.00	0.00		0.00	0.00	0.00
2'-CMP	0.00	0.00		0.00	0.00	0.00
CMP	0.00	0.00	0.00	0.00	0.00	0.00
UMP	0.00	0.00	0.00	0.00	0.00	0.00
N-Glycolylneuraminic acid	0.00	0.00	0.00	0.00	0.00	0.00
cAMP	0.00	0.00	0.00			0.00
Phytic acid_divalent	0.01	0.01	0.01	0.01	0.01	0.00
5-Aminoimidazole-4-carboxamide ribotide				0.00		0.00
Fructose 1,6-diphosphate	0.03	0.03	0.01	0.37	0.19	0.21
AMP	0.01	0.01	0.01	0.01	0.01	0.01
IMP	0.00	0.00	0.00	0.00	0.00	0.00
GMP	0.00	0.00	0.00	0.00	0.00	0.00
XA0055	0.00	0.00	0.00	0.02	0.01	0.01
NADPH_divalent	0.01	0.01	0.01	0.01	0.01	0.01
CoA_divalent	0.01	0.01	0.01	0.01	0.01	0.01
PRPP	0.00	0.00	0.00	0.00	0.00	0.00
FAD_divalent	0.00	0.00	0.00	0.00	0.00	0.00
CDP	0.00	0.00	0.00	0.00	0.00	0.00
UDP	0.01	0.01	0.01	0.01	0.01	0.01
Acetyl CoA_divalent	0.00	0.00	0.00	0.00	0.00	0.00
Thiamine diphosphate	0.00	0.00	0.00	0.00	0.00	0.00
Adenosine 5'-phosphosulfate	0.00	0.00		0.00	0.00	0.00
ADP	0.07	0.07	0.07	0.06	0.05	0.06
GDP	0.01	0.01	0.01	0.01	0.01	0.01
XA0065	0.00	0.00	0.00	0.00	0.00	0.00
HMG CoA_divalent	0.00	0.00	0.00	0.00	0.00	0.00
dCTP	0.00	0.00	0.00			
dTTP	0.00	0.00	0.00			
CTP	0.10	0.09	0.09	0.08	0.08	0.08
UTP	0.42	0.40	0.40	0.42	0.38	0.39
ATP	1.50	1.40	1.40	1.40	1.30	1.30
GTP	0.22	0.21	0.21	0.24	0.21	0.22
dTDP-glucose	0.00	0.00	0.00	0.00	0.00	0.00
UDP-glucose	0.21	0.20	0.20	0.28	0.22	0.24
UDP-galactose	0.21	0.20	0.20	0.28	0.22	0.24
UDP-glucuronic acid	0.06	0.06	0.05	0.06	0.06	0.06
ADP-glucose	0.00	0.00	0.00	0.00	0.00	0.00
GDP-fucose	0.00	0.00	0.00	0.00	0.00	0.00
GDP-glucose	0.00	0.00	0.00	0.00	0.00	0.00
GDP-mannose	0.00	0.00	0.00	0.00	0.00	0.00
GDP-galactose	0.00	0.00	0.00	0.00	0.00	0.00
UDP-N-acetylgalactosamine	0.15	0.13	0.14	0.09	0.09	0.09
UDP-N-acetylglucosamine	0.15	0.13	0.14	0.09	0.09	0.09
CMP-N-acetylneuraminate	0.01	0.01	0.01	0.01	0.01	0.01
NAD+	0.07	0.06	0.07	0.05	0.05	0.05
NADH	0.01	0.00	0.00	0.01	0.01	0.01
NADP+	0.01	0.00	0.00	0.01	0.00	0.00
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	KEGG ID	HMDB ID	quantified m/z	MT/RT
Pyruvic acid	C00022	HMDB00243	87.009	12.42
Lactic acid	C00186,C00256,C01432	HMDB00190,HMDB01311	89.025	10.41
3-Hydroxybutyric acid	C01089,C03197	HMDB00011,HMDB00357,HMDB00442	103.04	9.2
Glyceric acid	C00258	HMDB00139,HMDB06372	105.019	9.99
XA0002			110.985	15.13
Fumaric acid	C00122	HMDB00134	115.004	24.64
2-Oxoisovaleric acid	C00141	HMDB00019	115.041	9.81
Hexanoic acid	C01585	HMDB00535	115.077	8.62
Succinic acid	C00042	HMDB00254	117.02	20.3
Isethionic acid	C05123	HMDB03903	124.992	11.18
5-Oxoproline	C01879	HMDB00267	128.036	9.15
4-Methyl-2-oxovaleric acid	C00233	HMDB00695	129.057	9.23
3-Methyl-2-oxovaleric acid	C00671,C03465	HMDB00491	129.057	9.23
N-Acetyl-β-alanine	C01073		130.052	8.58
Malic acid	C00149,C00497,C00711	HMDB00156,HMDB00744	133.015	20.68
Threonic acid	C01620	HMDB00943	135.031	8.92
Ethanolamine phosphate	C00346	HMDB00224	140.013	7.72
Octanoic acid	C06423	HMDB00482	143.107	8.04
XA0004			144.031	8.98
2-Oxoglutaric acid	C00026	HMDB00208	145.015	20.72
2-Hydroxyglutaric acid	C02630,C01087,C03196	HMDB00606,HMDB00694	147.03	16.43
Pelargonic acid	C01601	HMDB00847	157.123	7.84
3-Hydroxy-3-methylglutaric acid	C03761		161.046	14.94
N-Acetylcysteine	C06809	HMDB01890	162.023	8.58
Terephthalic acid	C06337	HMDB02428	165.02	16.08
XA0012			166.019	9.06
Phosphoenolpyruvic acid	C00074	HMDB00263	166.975	20.44
Uric acid	C00366	HMDB00289	167.022	8.6
Glyceraldehyde 3-phosphate	C00118,C00661	HMDB01112	168.991	11.23
Dihydroxyacetone phosphate	C00111	HMDB01473	168.992	12.21
Glycerol 3-phosphate	C00093	HMDB00126	171.007	11.69
Decanoic acid	C01571	HMDB00511	171.14	7.67
cis-Aconitic acid	C00417	HMDB00072	173.01	27.21
N-Carbamoylaspartic acid	C00438	HMDB00828	175.036	14.94
O-Phosphoserine	C01005	HMDB00272	184.003	11.88
3-Phosphoglyceric acid	C00197	HMDB00807	184.986	19.01
2-Phosphoglyceric acid	C00631	HMDB03391	184.986	18.51
N-Acetylglutamine		HMDB06029	187.073	7.8
N-Acetylglutamic acid	C00624	HMDB01138	188.057	12.74
Isocitric acid	C00311	HMDB00193	191.019	27.9
Citric acid	C00158	HMDB00094	191.02	25.46
Quinic acid	C00296	HMDB03072	191.058	7.81
Glucuronic acid-1			193.036	7.94
Galacturonic acid-1			193.036	7.94
Glucuronic acid-2			193.036	7.8
Galacturonic acid-2			193.036	7.8
Gluconic acid	C00257	HMDB00625	195.052	7.9
Lauric acid	C02679	HMDB00638	199.171	7.4
Phosphocreatine	C02305	HMDB01511	210.029	11.86
O-Succinylhomoserine	C01118		218.068	11.77
Pantothenic acid	C00864	HMDB00210	218.105	7.4
XA0027			227.202	7.18
Ribose 5-phosphate	C00117	HMDB01548	229.012	10.16
Ribulose 5-phosphate	C00199,C01101	HMDB00618	229.012	10.53
XA0033			242.081	7.37
Glucose 1-phosphate	C00103	HMDB01586	259.024	9.75
Glucose 6-phosphate	C00668,C01172,C00092	HMDB01401	259.024	9.47
Fructose 6-phosphate	C05345,C00085	HMDB00124	259.024	9.58
myo-Inositol 1-phosphate	C01177	HMDB00213	259.024	9.89
myo-Inositol 3-phosphate	C04006	HMDB06814	259.024	9.89
myo-Inositol 2-phosphate			259.024	10.08
Sulfotyrosine			260.024	8.04
Sorbitol 6-phosphate	C01096	HMDB05831	261.038	9.58
2,3-Diphosphoglyceric acid	C01159	HMDB01294	264.953	17.91
6-Phosphogluconic acid	C00345	HMDB01316	275.018	14.14
Rhein	C10401		283.024	8.32
Sedoheptulose 7-phosphate	C05382	HMDB01068	289.035	9.33
N-Acetylglucosamine 1-phosphate	C04256	HMDB01367	300.05	9.2
N-Acetylglucosamine 6-phosphate	C00357	HMDB01062	300.05	8.83
N-Acetylneuraminic acid	C00270	HMDB00230	308.101	6.94
Ribulose 1,5-diphosphate	C01182		308.979	15.12
3'-CMP	C05822		322.045	9.42
2'-CMP	C03104	HMDB11692	322.045	9.42
CMP	C00055	HMDB00095	322.046	9.22
UMP	C00105	HMDB00288	323.03	9.41
N-Glycolylneuraminic acid	C03410	HMDB00833	324.095	6.92
cAMP	C00575	HMDB00058	328.045	7.24
Phytic acid_divalent	C01204	HMDB03502	328.924	15.91
5-Aminoimidazole-4-carboxamide ribotide	C04677	HMDB01517	337.054	9.1
Fructose 1,6-diphosphate	C00354	HMDB01058	338.99	14.06
AMP	C00020	HMDB00045	346.058	8.93
IMP	C00130	HMDB00175	347.04	9.18
GMP	C00144	HMDB01397	362.052	8.8
XA0055			369	13.79
NADPH_divalent	C00005	HMDB00221	371.54	10.87
CoA_divalent	C00010	HMDB01423	382.551	10.22
PRPP	C00119	HMDB00280	388.945	16.29
FAD_divalent	C00016	HMDB01248	391.572	7.6
CDP	C00112	HMDB01546	402.012	11.04
UDP	C00015	HMDB00295	402.997	11.22
Acetyl CoA_divalent	C00024	HMDB01206	403.558	9.87
Thiamine diphosphate	C00068	HMDB01372	423.031	7.66
Adenosine 5'-phosphosulfate	C00224	HMDB01003	426.015	9.77
ADP	C00008	HMDB01341	426.023	10.45
GDP	C00035	HMDB01201	442.018	10.25
XA0065			445.055	6.7
HMG CoA_divalent	C00356	HMDB01375	454.572	10.97
dCTP	C00458	HMDB00998	465.985	11.98
dTTP	C00459	HMDB01342	480.983	11.67
CTP	C00063	HMDB00082	481.978	11.91
UTP	C00075	HMDB00285	482.963	12.11
ATP	C00002	HMDB00538	505.989	11.38
GTP	C00044	HMDB01273	521.986	11.03
dTDP-glucose	C00842	HMDB01328	563.07	8.13
UDP-glucose	C00029	HMDB00286	565.05	8.32
UDP-galactose	C00052	HMDB00302	565.05	8.32
UDP-glucuronic acid	C00167	HMDB00935	579.029	10.61
ADP-glucose	C00498	HMDB06557	588.078	8.01
GDP-fucose	C00325	HMDB01095	588.078	8.01
GDP-glucose	C00394	HMDB03351	604.07	7.98
GDP-mannose	C00096	HMDB01163	604.07	7.98
GDP-galactose	C02280		604.07	7.98
UDP-N-acetylgalactosamine	C00203	HMDB00304	606.076	8.13
UDP-N-acetylglucosamine	C00043	HMDB00290	606.076	8.13
CMP-N-acetylneuraminate	C00128	HMDB01176	613.141	7.87
NAD+	C00003	HMDB00902	662.104	6.33
NADH	C00004	HMDB01487	664.12	7.88
NADP+	C00006	HMDB00217	742.07	9.02
METABOLITES_END
#END