#METABOLOMICS WORKBENCH KunZuo_20181227_232259 DATATRACK_ID:1593 STUDY_ID:ST001208 ANALYSIS_ID:AN002010
VERSION                          	1
CREATED_ON                       	03-03-2022
#PROJECT
PR:PROJECT_TITLE                 	Characterization of feces in Atrial Fibrillation (AF) patients
PR:PROJECT_SUMMARY               	Atrial Fibrillation (AF), an abnormal heart rhythm characterized by the rapid
PR:PROJECT_SUMMARY               	and irregular beating of the atria, is the most common arrhythmia with heavy
PR:PROJECT_SUMMARY               	global burdens. The present project aimed to characterized the feature of
PR:PROJECT_SUMMARY               	metabolites in feces of AF patients.
PR:INSTITUTE                     	Capital Medical University
PR:LAST_NAME                     	Zuo
PR:FIRST_NAME                    	Kun
PR:ADDRESS                       	beijing chaoyang hospital
PR:EMAIL                         	zuokun699@163.com
PR:PHONE                         	+861085231937
PR:DOI                           	http://dx.doi.org/10.21228/M8QM44
#STUDY
ST:STUDY_TITLE                   	Characterization of feces in Atrial Fibrillation (AF) patients
ST:STUDY_SUMMARY                 	Atrial Fibrillation (AF), an abnormal heart rhythm characterized by the rapid
ST:STUDY_SUMMARY                 	and irregular beating of the atria, is the most common arrhythmia with heavy
ST:STUDY_SUMMARY                 	global burdens. The present project aimed to characterized the feature of
ST:STUDY_SUMMARY                 	metabolites in feces of AF patients by using LC-MS.
ST:INSTITUTE                     	Capital Medical University
ST:LAST_NAME                     	Zuo
ST:FIRST_NAME                    	Kun
ST:ADDRESS                       	beijing chaoyang hospital
ST:EMAIL                         	zuokun699@163.com
ST:PHONE                         	010-85231937
ST:SUBMIT_DATE                   	2018-12-27
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	AF10	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF11	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF12	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF13	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF14	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF15	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF16	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF17	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF18	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF19	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF20	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF21	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF22	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF23	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF24	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF25	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF26	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF27	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF28	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF29	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF30	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF31	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF32	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF33	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF34	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF35	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF36	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF37	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF38	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF39	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF40	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF41	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF42	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF43	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF44	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF45	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF46	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF47	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF48	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF49	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF50	Factors:AF	
SUBJECT_SAMPLE_FACTORS           	-	AF9	Factors:AF	
#COLLECTION
CO:COLLECTION_SUMMARY            	Fresh stool samples were collected from each participant, immediately frozen at
CO:COLLECTION_SUMMARY            	−20 °C, transported on ice to the laboratory and then stored at −80 °C.
CO:SAMPLE_TYPE                   	Feces
#TREATMENT
TR:TREATMENT_SUMMARY             	We enrolled participants consisting of patients with AF.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	20 mg fecal samples were pipetted into centrifuge tubes (1.5 mL) in preparation
SP:SAMPLEPREP_SUMMARY            	for extraction. The protein was precipitated with 800 μL of methanol and 10 μL
SP:SAMPLEPREP_SUMMARY            	of internal standard (2.9 mg/mL, DL-o-Chlorophenylalanine) was added. The
SP:SAMPLEPREP_SUMMARY            	samples were ground at 65 KHz for 90 s and centrifuged at 12000 rpm for 15 min
SP:SAMPLEPREP_SUMMARY            	at 4 °C. 200 μL of the supernatant was transferred into a vial for further
SP:SAMPLEPREP_SUMMARY            	analysis.
#CHROMATOGRAPHY
CH:INSTRUMENT_NAME               	Thermo Dionex Ultimate 3000
CH:COLUMN_NAME                   	Hypersil Gold C18
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Deca LCQ
MS:INSTRUMENT_TYPE               	Ion trap
MS:MS_TYPE                       	ESI
MS:MS_COMMENTS                   	Compound Discoverer 2.0 software (Thermo)
MS:ION_MODE                      	POSITIVE
MS:MS_RESULTS_FILE               	ST001208_AN002010_Results.txt	UNITS:pg	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END