#METABOLOMICS WORKBENCH hjyoo2_20191230_224628_mwtab.txt DATATRACK_ID:1884 STUDY_ID:ST001297 ANALYSIS_ID:AN002161 PROJECT_ID:PR000878
VERSION             	1
CREATED_ON             	January 5, 2020, 6:08 pm
#PROJECT
PR:PROJECT_TITLE                 	High Cellulose diet impact on gut inflammation
PR:PROJECT_SUMMARY               	Dietary cellulose prevents gut inflammation by modulating lipid metabolism and
PR:PROJECT_SUMMARY               	gut microbiota
PR:INSTITUTE                     	Asan Medical Center
PR:LAST_NAME                     	Yoo
PR:FIRST_NAME                    	Hyun Ju
PR:ADDRESS                       	88, Olympic-ro, 43-gil, Songpa-gu, Seoul 05505, South Korea
PR:EMAIL                         	yoohyunju@amc.seoul.kr
PR:PHONE                         	82-02-3010-4029
#STUDY
ST:STUDY_TITLE                   	High Cellulose diet impact on gut inflammation
ST:STUDY_SUMMARY                 	Dietary cellulose prevents gut inflammation by modulating lipid metabolism and
ST:STUDY_SUMMARY                 	gut microbiota
ST:INSTITUTE                     	Asan Medical Center
ST:LAST_NAME                     	Yoo
ST:FIRST_NAME                    	Hyun Ju
ST:ADDRESS                       	88, Olympic-ro, 43-gil, Songpa-gu, Seoul 05505, South Korea
ST:EMAIL                         	yoohyunju@amc.seoul.kr
ST:PHONE                         	82-02-3010-4029
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	High_1	Cellulose(%):30	RAW_FILE_NAME=High_1.zip
SUBJECT_SAMPLE_FACTORS           	-	High_2	Cellulose(%):30	RAW_FILE_NAME=High_2.zip
SUBJECT_SAMPLE_FACTORS           	-	High_3	Cellulose(%):30	RAW_FILE_NAME=High_3.zip
SUBJECT_SAMPLE_FACTORS           	-	High_4	Cellulose(%):30	RAW_FILE_NAME=High_4.zip
SUBJECT_SAMPLE_FACTORS           	-	High_5	Cellulose(%):30	RAW_FILE_NAME=High_5.zip
SUBJECT_SAMPLE_FACTORS           	-	Low_1	Cellulose(%):0.3	RAW_FILE_NAME=Low_1.zip
SUBJECT_SAMPLE_FACTORS           	-	Low_2	Cellulose(%):0.3	RAW_FILE_NAME=Low_2.zip
SUBJECT_SAMPLE_FACTORS           	-	Low_3	Cellulose(%):0.3	RAW_FILE_NAME=Low_3.zip
SUBJECT_SAMPLE_FACTORS           	-	Low_4	Cellulose(%):0.3	RAW_FILE_NAME=Low_4.zip
SUBJECT_SAMPLE_FACTORS           	-	Low_5	Cellulose(%):0.3	RAW_FILE_NAME=Low_5.zip
#COLLECTION
CO:COLLECTION_SUMMARY            	Cecum contents were collected and freeze-dried for further analysis.
CO:SAMPLE_TYPE                   	Cecum
#TREATMENT
TR:TREATMENT_SUMMARY             	Freeze-dried cecum contents were stored at liquid N2 until metabolomics study.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Metabolites were extracted from cecum contents using conventional liquid-liquid
SP:SAMPLEPREP_SUMMARY            	extraction method. (Folch method) Internal standard were added to the samples
SP:SAMPLEPREP_SUMMARY            	before extraction. Nonpolar and polar metabolites were obtained from organic and
SP:SAMPLEPREP_SUMMARY            	aqueous phases, respectively.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	HILIC_neg
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Thermo Dionex Ultimate 3000
CH:COLUMN_NAME                   	Zorbax HILIC plus 3.5um, 2.1 x 100 mm
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	Xcaliber
MS:MS_RESULTS_FILE               	ST001297_AN002161_Results.txt	UNITS:cps	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END