#METABOLOMICS WORKBENCH ahmohamed_20200302_175121 DATATRACK_ID:1932 STUDY_ID:ST001323 ANALYSIS_ID:AN002201 PROJECT_ID:PR000900
VERSION             	1
CREATED_ON             	March 5, 2020, 9:58 pm
#PROJECT
PR:PROJECT_TITLE                 	Effect of high-fat diet on serum lipidome in mice
PR:PROJECT_SUMMARY               	We analyzed mouse serum samples from a mouse dietary intervention experiment.
PR:PROJECT_SUMMARY               	Briefly, C57BL/6 mice (n=44) were divided into 4 groups (n=11 per group) and fed
PR:PROJECT_SUMMARY               	High-fat diet (HFD), 1% deoxycholic acid (DCA) in drinking water, both, or left
PR:PROJECT_SUMMARY               	as control for 9 months. For quality control, 12 TQC samples and 2 blanks were
PR:PROJECT_SUMMARY               	also included in the analysis (total 58 samples and 6 groups). The two
PR:PROJECT_SUMMARY               	treatments were selected to demonstrate the ability of lipidomics to detect
PR:PROJECT_SUMMARY               	gross changes induced by HFD in the serum lipidome, as well as specific/minor
PR:PROJECT_SUMMARY               	changes induced by the secondary bile acid (DCA) through regulation of liver
PR:PROJECT_SUMMARY               	lipid metabolism.
PR:INSTITUTE                     	QIMR Berghofer Medical Research Institute
PR:DEPARTMENT                    	Precision & Systems Biomedicine
PR:LAST_NAME                     	Mohamed
PR:FIRST_NAME                    	Ahmed
PR:ADDRESS                       	300 Herston Road, Herston, QLD, 4006, Australia
PR:EMAIL                         	ahmed.mohamed@qimrberghofer.edu.au
PR:PHONE                         	+61738453992
#STUDY
ST:STUDY_TITLE                   	Effect of high-fat diet on serum lipidome in mice
ST:STUDY_SUMMARY                 	We analyzed mouse serum samples from a mouse dietary intervention experiment.
ST:STUDY_SUMMARY                 	Briefly, C57BL/6 mice (n=44) were divided into 4 groups (n=11 per group) and fed
ST:STUDY_SUMMARY                 	High-fat diet (HFD), 1% deoxycholic acid (DCA) in drinking water, both, or left
ST:STUDY_SUMMARY                 	as control for 9 months. For quality control, 12 TQC samples and 2 blanks were
ST:STUDY_SUMMARY                 	also included in the analysis (total 58 samples and 6 groups). The two
ST:STUDY_SUMMARY                 	treatments were selected to demonstrate the ability of lipidomics to detect
ST:STUDY_SUMMARY                 	gross changes induced by HFD in the serum lipidome, as well as specific/minor
ST:STUDY_SUMMARY                 	changes induced by the secondary bile acid (DCA) through regulation of liver
ST:STUDY_SUMMARY                 	lipid metabolism.
ST:INSTITUTE                     	QIMR Berghofer Medical Research Institute
ST:LAST_NAME                     	Mohamed
ST:FIRST_NAME                    	Ahmed
ST:ADDRESS                       	300 Herston Road
ST:EMAIL                         	ahmed.mohamed@qimrberghofer.edu.au
ST:PHONE                         	+61738453992
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENOTYPE_STRAIN               	C57BL/6
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	S1A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S1A.d
SUBJECT_SAMPLE_FACTORS           	-	S2A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S2A.d
SUBJECT_SAMPLE_FACTORS           	-	S3A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S3A.d
SUBJECT_SAMPLE_FACTORS           	-	S4A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S4A.d
SUBJECT_SAMPLE_FACTORS           	-	S5A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S5A.d
SUBJECT_SAMPLE_FACTORS           	-	S6A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S6A.d
SUBJECT_SAMPLE_FACTORS           	-	S7A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S7A.d
SUBJECT_SAMPLE_FACTORS           	-	S8A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S8A.d
SUBJECT_SAMPLE_FACTORS           	-	S9A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S9A.d
SUBJECT_SAMPLE_FACTORS           	-	S10A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S10A.d
SUBJECT_SAMPLE_FACTORS           	-	S11A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S11A.d
SUBJECT_SAMPLE_FACTORS           	-	S1B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S1B.d
SUBJECT_SAMPLE_FACTORS           	-	S2B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S2B.d
SUBJECT_SAMPLE_FACTORS           	-	S3B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S3B.d
SUBJECT_SAMPLE_FACTORS           	-	S4B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S4B.d
SUBJECT_SAMPLE_FACTORS           	-	S5B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S5B.d
SUBJECT_SAMPLE_FACTORS           	-	S6B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S6B.d
SUBJECT_SAMPLE_FACTORS           	-	S7B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S7B.d
SUBJECT_SAMPLE_FACTORS           	-	S8B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S8B.d
SUBJECT_SAMPLE_FACTORS           	-	S9B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S9B.d
SUBJECT_SAMPLE_FACTORS           	-	S10B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S10B.d
SUBJECT_SAMPLE_FACTORS           	-	S11B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S11B.d
SUBJECT_SAMPLE_FACTORS           	-	S1C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S1C.d
SUBJECT_SAMPLE_FACTORS           	-	S2C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S2C.d
SUBJECT_SAMPLE_FACTORS           	-	S3C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S3C.d
SUBJECT_SAMPLE_FACTORS           	-	S4C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S4C.d
SUBJECT_SAMPLE_FACTORS           	-	S5C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S5C.d
SUBJECT_SAMPLE_FACTORS           	-	S6C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S6C.d
SUBJECT_SAMPLE_FACTORS           	-	S7C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S7C.d
SUBJECT_SAMPLE_FACTORS           	-	S8C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S8C.d
SUBJECT_SAMPLE_FACTORS           	-	S9C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S9C.d
SUBJECT_SAMPLE_FACTORS           	-	S10C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S10C.d
SUBJECT_SAMPLE_FACTORS           	-	S11C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S11C.d
SUBJECT_SAMPLE_FACTORS           	-	S1D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S1D.d
SUBJECT_SAMPLE_FACTORS           	-	S2D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S2D.d
SUBJECT_SAMPLE_FACTORS           	-	S3D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S3D.d
SUBJECT_SAMPLE_FACTORS           	-	S4D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S4D.d
SUBJECT_SAMPLE_FACTORS           	-	S5D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S5D.d
SUBJECT_SAMPLE_FACTORS           	-	S6D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S6D.d
SUBJECT_SAMPLE_FACTORS           	-	S7D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S7D.d
SUBJECT_SAMPLE_FACTORS           	-	S8D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S8D.d
SUBJECT_SAMPLE_FACTORS           	-	S9D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S9D.d
SUBJECT_SAMPLE_FACTORS           	-	S10D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S10D.d
SUBJECT_SAMPLE_FACTORS           	-	S11D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S11D.d
SUBJECT_SAMPLE_FACTORS           	-	Blank_1	Diet:blank | BileAcid:blank	RAW_FILE_NAME=Blank_1.d
SUBJECT_SAMPLE_FACTORS           	-	Blank_2	Diet:blank | BileAcid:blank	RAW_FILE_NAME=Blank_2.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_1	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_1.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_2	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_2.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_3	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_3.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_4	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_4.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_5	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_5.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_6	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_6.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_7	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_7.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_8	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_8.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_9	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_9.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_10	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_10.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_11	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_11.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_12	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_12.d
#COLLECTION
CO:COLLECTION_SUMMARY            	Mouse serum lipids were extracted using the lipid extraction method by Matyash
CO:COLLECTION_SUMMARY            	et al(J Lipid Res 2008, 49, (5), 1137-46). Mouse serum (30 µL) was added to 215
CO:COLLECTION_SUMMARY            	µL of ice-cold methanol containing 50 µg/mL butylated hydroxytoluene (BHT).
CO:COLLECTION_SUMMARY            	Samples were homogenized by three rounds of vortex mixing for 30 seconds,
CO:COLLECTION_SUMMARY            	freezing in liquid nitrogen for one minute, thawing for two minutes and
CO:COLLECTION_SUMMARY            	sonicating for 10 minutes at 15°C, power 100% in a Grant XUB18 bath sonicator.
CO:SAMPLE_TYPE                   	Blood (serum)
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	C57BL/6 mice (n=44) were divided into 4 groups (n=11 per group) and fed High-fat
TR:TREATMENT_SUMMARY             	diet (HFD), 1% deoxycholic acid (DCA) in drinking water, both, or left as
TR:TREATMENT_SUMMARY             	control for 9 months. Serum samples were collected and analyzed by targeted
TR:TREATMENT_SUMMARY             	lipidomics as described in Supplemental Information. The two treatments were
TR:TREATMENT_SUMMARY             	selected to demonstrate the ability of lipidomics to detect gross changes
TR:TREATMENT_SUMMARY             	induced by HFD in the serum lipidome, as well as specific/minor changes induced
TR:TREATMENT_SUMMARY             	by the secondary bile acid (DCA) through regulation of liver lipid metabolism.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Mouse serum lipids were extracted using the lipid extraction method by Matyash
SP:SAMPLEPREP_SUMMARY            	et al. Mouse serum (30 µL) was added to 215 µL of ice-cold methanol containing
SP:SAMPLEPREP_SUMMARY            	50 µg/mL butylated hydroxytoluene (BHT). Samples were homogenized by three
SP:SAMPLEPREP_SUMMARY            	rounds of vortex mixing for 30 seconds, freezing in liquid nitrogen for one
SP:SAMPLEPREP_SUMMARY            	minute, thawing for two minutes and sonicating for 10 minutes at 15°C, power
SP:SAMPLEPREP_SUMMARY            	100% in a Grant XUB18 bath sonicator. SPLASH LipidoMix Mass Spec Standard (10
SP:SAMPLEPREP_SUMMARY            	µL, undiluted) and Cer/Sph mixture II (10 µL, undiluted) internal standards
SP:SAMPLEPREP_SUMMARY            	mixes from Avanti Polar Lipids were then added to each sample. After overnight
SP:SAMPLEPREP_SUMMARY            	incubation at -30°C, 750 µL MTBE was added and each tube was vortex mixed for
SP:SAMPLEPREP_SUMMARY            	10 seconds and shaken for 10 minutes on a tube rotator (4°C). MilliQ water (188
SP:SAMPLEPREP_SUMMARY            	µL) was then added, and the tube was vortex mixed for 30 seconds to form a
SP:SAMPLEPREP_SUMMARY            	biphasic separation. After centrifuging for 15 minutes at 15,000 ×g, the clear
SP:SAMPLEPREP_SUMMARY            	upper phase containing lipids in MTBE was transferred to another tube and dried
SP:SAMPLEPREP_SUMMARY            	down using a gentle stream of nitrogen. Prior to LC-MS/MS analysis, the samples
SP:SAMPLEPREP_SUMMARY            	were resuspended in a mixture of 50 µL methanol (containing 50 µg/mL
SP:SAMPLEPREP_SUMMARY            	BHT)/toluene (90/10%, v/v) and 4µL was used as the injection volume.
SP:PROCESSING_STORAGE_CONDITIONS 	On ice
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	buffer A contained 50% acetonitrile and buffer B contained 95% acetonitrile in
CH:CHROMATOGRAPHY_SUMMARY        	water. Buffers were supplemented with 10 mM ammonium acetate (pH 7.6).
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity
CH:COLUMN_NAME                   	Agilent HILIC Plus RRHD (100 x 2.1mm, 1.8um)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent 6490 QQQ
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	UNSPECIFIED
MS:MS_COMMENTS                   	Targeted lipidomics were performed on an Agilent Technologies 1290 Infinity
MS:MS_COMMENTS                   	UHPLC system with an Agilent HILIC Plus RRHD 2.1×100 mm 1.8-micron column,
MS:MS_COMMENTS                   	coupled online to an Agilent 6490A Triple Quadrupole mass spectrometer with
MS:MS_COMMENTS                   	iFunnel and Agilent Jet Stream (AJS) electrospray ionization (ESI) source,
MS:MS_COMMENTS                   	operated in dynamic MRM mode. The source nitrogen gas temperature was set to
MS:MS_COMMENTS                   	250°C at a flow of 15 L/min and a sheath gas temperature of 400°C at a flow of
MS:MS_COMMENTS                   	12 L/min. The capillary voltage was set to 4000 V for positive mode and 5000 V
MS:MS_COMMENTS                   	for negative mode and the nebulizer operated at 30 psi. Ion funnel high and low
MS:MS_COMMENTS                   	pressure in positive and negative mode were 150 and 120. Check tunes were
MS:MS_COMMENTS                   	performed in wide, unit and enhanced modes prior to each experiment to confirm
MS:MS_COMMENTS                   	the performance of the mass spectrometer. The quadrupole was tuned to reference
MS:MS_COMMENTS                   	masses 118.09, 322.05, 622.03, 922.01 and 1221.99 in positive ionization mode;
MS:MS_COMMENTS                   	112.99, 302.00, 601.98, 1033.99 and 1333.97 in negative ionization mode.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	intensity
MS_METABOLITE_DATA_START
Samples	S1A	S2A	S3A	S4A	S5A	S6A	S7A	S8A	S9A	S10A	S11A	S1B	S2B	S3B	S4B	S5B	S6B	S7B	S8B	S9B	S10B	S11B	S1C	S2C	S3C	S4C	S5C	S6C	S7C	S8C	S9C	S10C	S11C	S1D	S2D	S3D	S4D	S5D	S6D	S7D	S8D	S9D	S10D	S11D	Blank_1	Blank_2	TQC_1	TQC_2	TQC_3	TQC_4	TQC_5	TQC_6	TQC_7	TQC_8	TQC_9	TQC_10	TQC_11	TQC_12
Factors	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:blank | BileAcid:blank	Diet:blank | BileAcid:blank	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC
PE 32:0	13540	15873	16004	21718	18040	20555	19578	22969	25406	26887	38785	25639	14862	15274	11281	15713	13261	16947	24734	32983	22646	20656	14708	17053	12702	16282	9460	15623	12730	15403	16621	16456	25000	8064	10538	22046	15093	14742	14740	24697	7399	9501	12555	14109	1	2	16412	14236	15305	16218	16464	13521	13576	14299	14016	14736	18405	18760
PE 32:1	11814	12774	11583	21800	14787	21027	17008	11133	25511	43005	36986	9297	13729	14479	25038	22518	10902	21644	45249	44150	56266	40978	4115	4981	6429	7314	4961	6328	8544	5683	5559	9006	11117	5397	16741	10123	8234	8967	9751	13725	9373	10828	10649	10788	0	1	13770	12132	13432	13272	12630	12651	14115	14711	11811	12284	14258	15010
PE 32:2	384	1276	2228	2403	2998	4537	906	3095	4864	4491	4605	1049	2239	1973	3996	2413	2381	3003	5178	4624	5594	4428	496	848	361	771	227	1664	334	840	1550	1430	1316	1524	468	1782	1185	1366	1921	1287	627	983	1130	2081	0	1	755	2206	2436	983	1845	2878	2494	1465	2389	1802	1434	2103
PE 34:1	119262	160307	151440	237403	170406	250896	187078	132692	299280	371385	299284	171017	179147	252282	260609	222094	179798	183581	409211	350526	497671	336603	82823	111378	89992	101969	81575	102342	101934	100218	115325	129287	149276	95914	163749	159312	123326	125497	119581	143748	80484	128073	132290	155749	0	1	175336	160041	145836	149014	143375	153687	181767	171089	169558	160829	159982	163079
PE 34:1 NEG	2002	2881	1167	4272	2606	5649	1850	3108	3709	4286	5225	2580	2240	5614	3488	2592	4021	2601	6779	7092	8308	6623	1224	1022	2668	1270	1945	1664	1916	1183	1552	3142	3260	758	2412	3153	1568	1854	1878	2283	790	1774	2490	1506	65	148	1989	2145	1657	3082	3045	2157	2627	2403	2535	2706	3148	2516
PE 34:2	240388	350214	308289	521102	404785	523376	315632	270907	493603	823324	631246	279354	352711	340947	414837	336137	358000	290892	456830	471028	588884	382259	196801	178714	197464	187675	106691	189546	167323	244628	198300	281246	248277	217767	321033	374286	333978	239258	308104	309377	160530	243672	289275	322936	221	0	317750	298507	286596	339583	303071	310214	261767	323342	323657	312723	313084	310028
PE 34:3	14765	23099	22590	37375	30811	34758	25377	18986	40050	55014	42647	19496	23765	28731	31097	28296	28186	23074	38954	41343	53928	44019	10801	10214	9268	11464	5383	9471	10907	10067	11542	15819	14057	11743	22414	18827	13208	11329	13801	15574	15876	17789	17778	15282	1	NA	22834	19971	20526	20561	17262	18968	16119	20805	21484	22730	21434	19730
PE 36:0	67525	53432	45490	77827	53165	59218	52115	49198	45556	63866	70769	39461	42021	34765	34402	37252	26350	38767	35331	40173	45394	47012	57991	54344	47473	68062	46795	56022	39558	57357	44750	49945	55919	31311	37544	45458	44291	36790	39861	38732	54195	33254	30992	39504	2	NA	50181	48249	49730	47947	43935	46658	50813	50797	39863	50157	51478	55748
PE 36:1	133630	141073	230844	212196	182220	256625	163004	111343	258536	237118	280372	145084	143696	166141	129637	160024	175383	123371	179639	203973	190788	173819	156200	193809	137859	142820	92348	151283	105910	218578	158874	222167	173669	123808	162750	260507	247055	128681	151609	148023	63252	129071	153945	159813	38	59	171541	169569	124168	135683	149408	146966	144603	143645	149538	160119	148131	131450
PE 36:1 NEG	10054	12451	12952	15699	13222	19186	7197	5778	18051	13495	18078	9949	9772	8913	6829	9191	10874	8117	11158	13562	12129	10821	11516	7681	9760	10068	5175	8224	6531	9751	10994	15212	10501	11570	10282	15324	9494	6956	12185	9712	6126	4774	9070	7897	160	282	7380	12049	8333	8093	7954	8566	6917	13501	8868	7740	11323	9319
PE 36:2	502985	468182	902580	819814	576836	967774	530903	414950	878485	957387	1021789	564868	487302	627579	664313	641900	835233	555877	708465	697181	799169	644876	579609	604450	766737	549770	426350	657323	629127	761173	568104	910979	730510	629304	836101	1344113	1001966	752002	813138	902252	401811	690990	772465	780713	711	342	782135	642117	677912	627678	709303	757627	627800	685177	668908	632874	689787	707441
PE 36:3	171602	223064	284784	270575	240791	335802	230144	200775	390848	445971	449702	247884	212695	229508	214865	217465	286433	208581	256739	285510	295668	258012	128253	168971	193579	148730	120087	143883	138428	167291	162395	246806	171285	193742	262146	296316	223767	172564	227029	259955	179374	200419	211976	255524	70	175	221094	238140	229663	239150	209617	248202	199438	219171	208953	221824	232419	228292
PE 36:4	264299	262717	322651	378113	313693	395507	292631	290927	472109	606227	543490	144803	165597	181755	228909	144295	195704	165931	267987	248563	308412	248874	190481	203736	288039	291832	195719	246966	206278	238899	254078	293709	306516	172600	261392	321743	310508	355595	237356	278059	337862	314922	325904	318987	144	64	291361	299290	274938	258103	333581	259751	245273	303055	275875	324358	284539	300778
PE 36:5	32029	46245	27330	60119	56316	47370	47427	46440	68303	72387	83148	20615	32798	21495	50377	27889	22530	24756	47029	50572	53825	46996	15009	14480	17066	19980	14320	18739	11693	17997	15386	18979	25126	13876	25415	24197	18547	24670	21070	22301	26266	32412	24380	28475	2	0	28971	34354	29166	35154	33510	36564	29262	30765	32802	26966	32641	30795
PE 38:0	47173	44276	44440	58159	52893	54295	43387	43468	39110	62829	52774	37885	40439	33552	35739	29294	22827	34868	31257	32689	30743	35684	71296	53985	59512	74194	52696	69641	49479	66162	54924	59688	60174	37724	42086	55596	46930	46501	36752	37005	36593	33193	41144	39926	NA	NA	52979	50846	46941	57853	45177	56485	57381	54220	44696	46081	46231	40817
PE 38:1	22898	15576	18787	16963	20353	15539	19964	16654	17016	25127	24570	15374	15397	12628	11744	12019	11820	14643	10675	19889	15169	13947	16237	15282	13534	18287	12260	12877	9579	15170	11968	12401	14960	12688	9601	15570	11375	17004	12557	14408	13671	14313	12321	11309	73	48	13974	16803	16531	16842	15826	17270	16292	17192	12157	14592	16577	17933
PE 38:2	18560	19676	21204	29317	21483	20102	19354	21279	31221	31643	32517	15165	15903	18313	18768	15446	21968	15468	26490	26003	25381	22061	12715	12964	12948	12985	7192	11399	10279	9127	11951	12742	12475	10614	10962	14311	12993	11534	13702	14437	12227	11498	9895	13902	60	NA	15206	13821	15650	17735	14758	19673	8392	14352	15695	15104	15213	14882
PE 38:3	74782	79298	84454	101039	86084	79188	83713	68075	115887	131873	115868	52295	57701	57959	60901	52356	84131	59074	74834	78770	86252	74335	80521	89011	98387	101383	60307	93525	81041	105722	93113	102821	112116	71420	97539	121437	96808	90413	83967	101515	82176	95423	107275	98491	NA	65	100736	109902	98904	84887	96433	100982	89332	96503	82760	88635	92804	92206
PE 38:4	341871	331984	388131	403325	331863	323046	345183	322718	434010	595650	539793	199444	235319	196716	252171	152759	261572	215659	241115	253251	259722	246476	428426	507461	570135	571085	408535	653307	457943	712783	578469	700454	658511	404857	541735	790473	600282	642127	529735	544438	491978	556448	653373	559688	147	300	587005	539441	477530	502059	503043	536402	543241	598987	469173	502419	519601	479076
PE 38:5	186705	197419	234863	235619	227670	209039	240009	229697	307191	388435	471674	120009	117363	143491	164420	92689	126974	168314	193972	215035	203324	189861	168094	166213	164366	223132	139405	178471	149296	158613	188591	213803	214076	122581	206816	205137	187702	212409	159169	236453	271963	245433	218204	237993	239	241	215097	221921	240381	211894	218032	205684	227332	243370	183452	215550	201802	223201
PE 38:6	397695	474634	414818	487774	428746	395342	431129	392985	640187	618485	826667	173832	280960	316022	379533	226367	273421	340607	422562	427678	404784	388926	217379	306433	255609	405985	228506	259547	170734	299078	275138	342387	278169	216822	413517	403419	373816	412630	261549	289126	568146	474181	427813	476146	280	20	412669	370810	399081	411689	332112	395032	393185	392574	418698	339303	352602	329041
PE 40:0	11628	9622	8724	11803	11196	12549	11839	10688	10220	13069	10880	7487	8131	7340	6470	6107	5318	6846	6536	9332	6895	7638	13174	11659	10744	14870	7004	7884	7327	10799	10631	9438	10099	7770	9839	9043	9794	7444	5889	8433	9731	6695	9053	9589	NA	5	9497	11145	10286	9776	10781	11295	8252	12298	10131	8730	7626	7234
PE 40:5	37596	40860	53781	71328	52179	50782	59076	47537	69735	71762	113957	29228	29898	35147	34844	19503	36944	36795	39803	53438	50289	42362	39683	37480	41914	52840	28391	32906	33359	41963	37173	34440	43534	33624	47593	54700	55829	44161	33397	53130	46674	45963	54608	64015	264	NA	59237	51231	42299	48863	50902	56321	49288	41652	34873	41918	41676	33021
PE 40:6	112574	129035	118277	138463	122526	98995	137517	130848	168181	203897	201328	65726	90483	89105	97003	61841	104160	81444	101477	121778	126283	117822	80210	124244	96520	127523	73477	99811	69303	98279	129691	135713	126882	119534	131525	157575	134257	158338	114815	137263	148037	156178	144118	159355	115	10	138027	141436	140069	138577	173757	142981	129305	138604	144534	132996	103160	105435
PE 40:7	64496	92211	83220	77429	85386	53705	84776	94178	120238	134880	186728	64589	51624	83292	89160	44617	79101	122376	82805	104606	86378	89681	38870	41811	48459	69111	48372	44508	35287	45955	53615	66163	53377	47564	62668	72894	62443	71318	62473	89435	115327	92231	80275	109011	31	44	87142	72669	92619	76133	73820	71594	76788	76470	83713	81666	83900	64169
PE(O-34:0)	18938	22723	19038	28056	25923	26690	18274	19452	21982	25686	25791	20733	17404	13864	16548	14079	12526	13096	12928	18525	14550	16617	24376	27266	16391	29954	18457	16908	15645	22397	18279	27414	14188	11433	12357	17449	14950	13177	12364	12503	11437	11414	15255	15712	37	151	19015	15619	15421	14404	15192	17177	19590	17413	13093	17935	15318	16811
PE(O-34:1)	70579	64335	62585	93817	76584	82041	60305	54718	60555	93614	78938	79708	82931	62760	60392	65264	53168	68321	78901	84283	67653	89621	62325	56962	46514	55162	38374	45323	37618	47437	42405	50271	44988	29389	47232	42038	38075	25911	37102	37571	40423	24746	29131	33528	59	69	66085	54304	47645	52990	62633	57368	58724	60573	60790	50071	58151	54142
PE(O-34:2)	53344	52505	43847	75809	49469	47018	41557	42683	42228	71884	57004	61304	44372	37352	40987	43772	40555	45978	46280	38441	38923	59156	29835	23415	22503	26535	17749	21851	26343	26370	19493	22128	20422	15730	24301	18250	21691	18605	19130	13603	21235	14689	15162	18847	56	NA	35165	33375	40980	35498	35963	34134	37696	44269	39166	35710	38098	45003
PE(O-36:0)	5246	8293	6203	11001	7861	11037	9244	8616	8191	9736	10415	7335	3929	5920	3957	5160	6746	7260	7626	8176	7582	7661	10935	12664	7073	11161	7824	7197	6491	9163	7406	9208	5837	7430	6954	7555	6149	5491	4253	5079	8085	4946	6286	6602	38	33	7878	6893	5260	6655	9013	8351	6404	8433	10468	5046	7568	6623
PE(O-36:1)	75436	83693	68062	87977	84918	81609	80411	67749	61065	81088	65562	79788	75383	53505	49110	58184	54075	56932	83174	61824	63248	57896	98296	92515	73572	106783	65186	71729	56088	89141	80364	88233	52318	55553	60800	58898	48945	50249	45163	42348	55238	48677	49025	58138	62	NA	67271	60213	61110	57600	54255	65454	71723	60698	65611	71647	62774	64728
PE(O-36:2)	146030	137015	114313	151756	130483	136663	122965	99214	107140	143674	116907	133814	122086	103814	98565	106061	105048	105184	103668	112965	94456	128821	120100	130834	119712	158304	92277	110150	106900	141312	105071	121108	88680	74798	102758	112118	95988	74181	74386	78875	83695	71181	89453	114417	308	174	113299	128539	127877	108368	120036	110079	113135	112957	103344	104396	108663	106753
PE(O-36:3)	50905	54727	40136	67769	57549	55539	49501	45905	41550	60142	50489	52408	53160	34043	39830	39191	30855	41130	40176	38481	37205	56740	48400	43020	41093	47498	31465	35346	26666	41046	32264	44598	26504	25580	30784	27519	25568	26647	28312	30451	27736	24210	25615	26516	NA	NA	51280	43111	42578	42937	38785	41213	41953	43419	38380	34774	39862	42533
PE(O-36:4)	54920	42469	34662	36844	27665	32711	30974	32393	28181	37528	45647	35493	36993	35833	30049	26639	26138	33661	32570	36265	36434	38154	68709	46400	56669	52185	44704	59606	58278	45865	50985	49262	53109	29755	34776	42072	36168	33943	38245	36378	31901	33382	37803	27196	129	71	39509	38219	48651	40640	44954	42510	50122	41125	52158	51248	39111	43102
PE(O-36:5)	26512	27246	18922	23727	21688	20132	15075	18941	16148	25569	21534	14762	18206	16089	20082	15110	12045	13648	17029	16024	20315	16240	26326	22509	19965	22692	21321	26365	26184	25648	19129	22551	21987	15190	12932	16944	19671	22095	19358	12303	18778	17834	14205	15308	1	64	22951	21089	24536	22453	23983	20751	23094	22854	22673	20579	19492	22134
PE(O-38:0)	23797	30206	25359	32099	33938	27639	32455	27901	38594	38945	38156	18672	22584	23192	25185	18050	20624	24804	29546	33539	28558	29716	19249	22071	17507	27901	17089	14822	13957	18502	18987	24834	16930	14705	22628	20018	21345	20238	20295	20228	27010	21868	18757	20523	NA	77	24081	29050	22518	25699	20664	25205	21276	22427	23661	23380	23215	26087
PE(O-38:1)	47959	64238	61727	66883	78856	70033	76940	52431	55312	66179	49398	50962	61199	51927	39831	47593	41757	51450	43145	50826	47613	43671	57724	74667	47474	67989	42877	46842	31736	43815	41578	59201	42660	37687	43709	51270	43717	41390	32707	32655	38319	38227	40857	46423	9	NA	49728	47238	46387	47236	55097	41661	47942	41866	48372	47901	47022	43863
PE(O-38:2)	79605	108040	126415	111549	97834	102987	101496	77448	82664	97384	81904	91124	90235	75639	55071	67021	66168	68307	76353	67648	65871	70855	63880	97429	57665	86892	57457	54919	49724	66341	55048	71897	47450	50518	66634	69297	53887	49787	57720	58786	59136	49885	49720	62099	3	NA	72558	69817	75587	74141	75616	70354	73398	63824	71319	68088	73794	67358
PE(O-38:3)	41387	46414	45149	50358	53941	51078	52779	37433	41119	46729	39925	38122	40462	33637	25227	37686	29307	33355	31022	37355	27451	33274	71541	82351	60085	81863	52566	59956	44784	65155	58405	61258	54423	37772	45481	47936	51300	43003	35953	48171	34698	36040	38931	40432	200	1	45778	46741	53405	45508	52563	47084	51389	50426	48497	47456	46613	45408
PE(O-38:4)	218099	147098	151419	156423	142078	141331	136995	132293	112123	126821	154779	130865	133210	107881	79033	105657	84424	101439	96157	92475	99970	88017	474434	571384	378959	524025	395457	416454	375816	511305	413803	503763	347654	234950	229661	301966	292563	288823	248199	255298	218000	192492	207580	233929	63	96	295368	278346	286704	269514	300486	277315	262201	254823	246084	278330	246626	251181
PE(O-38:5)	96460	83192	56914	108034	64228	101518	71959	69900	73684	91463	95273	64914	75861	66861	64406	76763	50195	65808	69856	69833	56637	78381	162364	78705	122916	115439	74543	91339	92876	103865	86448	71479	89115	65142	75343	107133	104310	80240	74718	73242	53711	57133	69927	77345	53	68	106203	96187	95965	100188	67182	87377	104942	81769	89113	87186	86194	77859
PE(O-40:5)	106541	143352	101258	137997	159670	114362	135010	102259	115569	120258	126654	96536	111757	96135	82152	77236	64935	78086	79035	100644	77181	75677	273059	263208	222696	282406	169680	175720	159052	239907	150530	229096	145584	144498	151010	171246	140190	137395	132889	152639	127144	106247	124971	124877	113	71	147262	130245	150652	151941	145028	137415	158497	163091	149141	135346	135564	131640
PE(O-40:6)	145127	151034	134277	149442	113699	141230	102354	106336	100761	149198	155156	125060	128386	115793	83968	109731	83544	102389	105264	84869	97109	108346	187961	203828	167446	247064	177472	214408	162799	257114	203038	219451	184410	135063	161910	193720	153757	155023	174450	161415	133184	115661	127401	135486	58	24	153284	135903	158311	152868	160353	137561	161195	135193	163366	143275	133502	148003
PE(O-40:7)	44610	40631	42687	58955	46171	45188	39405	45110	47476	67670	51006	27238	37660	30382	31998	31516	23772	31012	30722	31670	26920	31164	76795	61642	46991	72956	47306	50624	45272	62262	47655	60129	56481	33219	44774	49414	41749	39438	35428	44011	43427	34711	42566	33169	NA	33	49117	43008	43070	47598	44731	49984	44327	51662	43455	44755	41251	46650
PE(P-32:0)	4132	4100	2987	6396	5171	3414	3977	3965	6347	5301	5732	5844	4502	5838	3754	6092	4505	5659	7302	4167	5489	5464	2652	3393	1552	3092	1387	2168	2606	1561	1760	2783	1381	1118	2981	2348	2348	1907	2738	1961	1372	2324	1299	2247	113	51	3891	2953	4012	3752	3603	3013	3302	2746	4981	4369	4049	4037
PE(P-34:0)	49378	41078	41002	57494	49671	58604	41518	39776	42706	68904	56228	49261	44609	39450	39786	41166	40793	49711	58259	44832	52350	57812	42370	33703	28421	31663	26167	35890	27599	31927	23699	36888	23827	20198	29170	24289	22650	19650	20295	19365	21798	22479	22080	28960	44	48	39085	37907	30783	35059	38320	33711	44093	38812	37646	39175	37228	38174
PE(P-34:1)	53344	52505	43847	75809	49469	47018	41557	42683	42228	71884	57004	61304	44372	37352	40987	43772	40555	45978	46280	38441	38923	59156	29835	23415	22503	26535	17749	21851	26343	26370	19493	22128	20422	15730	24301	18250	21691	18605	19130	13603	21235	14689	15162	18847	56	NA	35165	33375	40980	35498	35963	34134	37696	44269	39166	35710	38098	45003
PE(P-34:2)	18747	17510	11722	24926	15995	16679	13348	16023	14983	22129	18548	14264	11904	6171	12663	12177	8033	11534	10144	11489	14302	16170	10392	8092	8021	7864	6659	6875	7806	7701	6932	7063	8417	6337	6484	6658	7078	3362	7013	4345	4276	3819	5265	5799	3	0	7571	10435	10787	10426	9717	9687	11402	11391	11652	12011	8663	12348
PE(P-36:0)	75436	83693	68062	87977	84918	81609	80411	67749	61065	81088	65562	79788	75383	53505	49110	58184	54075	56932	83174	61824	63248	57896	98296	92515	73572	106783	65186	71729	56088	89141	80364	88233	52318	55553	60800	58898	48945	50249	45163	42348	55238	48677	49025	58138	62	NA	67271	60213	61110	57600	54255	65454	71723	60698	65611	71647	62774	64728
PE(P-36:1)	146030	137015	114313	151756	130483	136663	122965	99214	107140	143674	116907	133814	122086	103814	98565	106061	105048	105184	103668	112965	94456	128821	120100	130834	119712	158304	92277	110150	106900	141312	105071	121108	88680	74798	102758	112118	95988	74181	74386	78875	83695	71181	89453	114417	308	174	113299	128539	127877	108368	120036	110079	113135	112957	103344	104396	108663	106753
PE(P-36:2)	50905	54727	40136	67769	57549	55539	49501	45905	41550	60142	50489	52408	53160	34043	39830	39191	30855	41130	40176	38481	37205	56740	48400	43020	41093	47498	31465	35346	26666	41046	32264	44598	26504	25580	30784	27519	25568	26647	28312	30451	27736	24210	25615	26516	NA	NA	51280	43111	42578	42937	38785	41213	41953	43419	38380	34774	39862	42533
PE(P-36:3)	53579	42027	34503	36392	27115	32434	30803	32091	28005	37462	45727	35128	36777	35701	29983	26307	25398	33570	32232	35921	35679	38072	68340	46310	56363	51982	44096	59200	56551	45558	50327	47539	51300	29459	34537	41988	36022	33543	37807	33912	31798	32881	36630	27133	73	67	38829	37681	47771	40467	44940	42297	49704	41002	51956	50160	39103	41823
PE(P-36:4)	26223	27205	19027	23729	21607	20142	15024	18913	16099	25053	21420	14726	18459	16063	19999	15114	11864	13639	17029	15904	20298	16220	26257	22401	19500	22372	20922	25859	25987	25625	18993	22507	21933	15021	12788	16725	19729	21854	19495	12256	18720	17794	14165	15279	NA	64	22947	21089	24534	22450	23984	20707	22820	22171	22577	20583	19430	22332
PE(P-38:0)	34033	49634	41799	42162	54432	46105	48727	36750	40883	48425	43565	40497	41605	39163	25787	24858	25136	35858	33878	32814	33924	31719	41154	44917	29220	53682	26496	31620	22072	34846	28217	36076	27409	22545	31458	34666	27926	24450	28197	20139	25756	25894	34531	34122	45	57	35046	34976	29014	26688	38076	34047	35177	36107	36097	32612	38042	33355
PE(P-38:1)	65991	66576	64651	82796	79329	82584	73096	59979	62876	80083	68519	67447	58086	51860	46147	58209	48011	52012	51120	63809	51954	50729	45698	58911	40875	63006	34493	39657	31386	43465	37637	64055	37797	34644	42933	47973	47084	38284	36363	38240	38566	31369	40013	50088	291	70	51909	48870	46305	56213	56899	44625	56555	54639	50013	52704	57013	58672
PE(P-38:2)	35680	39079	32352	36086	41457	40316	39255	30803	35114	41849	33778	31498	31034	31254	19124	27799	21788	25654	29647	24678	25582	24589	57588	62560	50715	68714	40996	52855	40187	50316	50919	54832	44577	31744	32656	45702	37196	31853	34223	36273	25853	25152	35095	33387	68	328	48722	40519	34692	41079	43195	38585	38227	43774	33378	34756	39556	34249
PE(P-38:3)	166590	126134	135752	145557	149214	117484	114405	120850	103281	132365	111385	115508	103338	112277	77205	81235	67612	90369	109494	73904	79237	78471	426235	467954	335514	465988	349181	388682	284845	387216	332204	404216	298249	194749	194015	256537	282732	258675	210281	228257	182527	177935	185066	181479	50	96	245130	219082	235288	211678	225197	221754	227810	237811	209942	200333	236697	188242
PE(P-38:4)	96460	83192	56914	108034	64228	101518	71959	69900	73684	91463	95273	64914	75861	66861	64406	76763	50195	65808	69856	69833	56637	78381	162364	78705	122916	115439	74543	91339	92876	103865	86448	71479	89115	65142	75343	107133	104310	80240	74718	73242	53711	57133	69927	77345	7	57	106203	96187	95965	100188	67182	87377	104942	81769	89113	87186	86194	77859
PE(P-38:5)	185832	153316	160454	232303	108179	167752	112054	106178	108939	141991	180500	159701	204702	143295	126788	148712	132988	142870	139268	153486	137910	183138	221604	146180	151785	149824	122785	162189	139941	210707	142807	163541	187915	117287	131297	131838	129750	114518	126946	117102	112324	85902	110930	112081	40	64	173799	148927	163710	160054	174149	169913	191253	177171	149509	138853	176968	160363
PE(P-38:6)	44946	47236	38464	70619	38965	49500	33570	39285	34023	55065	47264	31364	39127	27255	26512	30109	19524	27092	27692	33349	39811	33907	59493	39513	41433	40566	28619	47083	30063	57482	33425	38293	49313	17392	36547	40249	33384	31549	23497	34009	31061	25501	25465	29382	57	38	38389	44714	43753	38418	35102	43066	47366	44424	35141	40292	40236	36784
PE(P-40:5)	145127	151034	134277	149442	113699	141230	102354	106336	100761	149198	155156	125060	128386	115793	83968	109731	83544	102389	105264	84869	97109	108346	187961	203828	167446	247064	177472	214408	162799	257114	203038	219451	184410	135063	161910	193720	153757	155023	174450	161415	133184	115661	127401	135486	58	24	153284	135903	158311	152868	160353	137561	161195	135193	163366	143275	133502	148003
PE(P-40:6)	44610	40631	42687	58955	46171	45188	39405	45110	47476	67670	51006	27238	37660	30382	31998	31516	23772	31012	30722	31670	26920	31164	76795	61642	46991	72956	47306	50624	45272	62262	47655	60129	56481	33219	44774	49414	41749	39438	35428	44011	43427	34711	42566	33169	NA	33	49117	43008	43070	47598	44731	49984	44327	51662	43455	44755	41251	46650
PE 15:0/18:1(d7)	150403	132031	136162	147958	115590	110000	132882	155683	86824	130874	122428	124734	155020	165930	133624	125309	120953	160805	123437	147405	123433	118739	145169	139661	144049	148098	117581	102882	144315	138086	111058	89493	131760	111277	118099	139508	130981	140714	208043	107748	157361	128130	128847	150196	29	57	356	318	656	258	69	117	642	664	825	338	763	599
PG 15:0/18:1(d7)	1269565	1220721	1048806	1279516	1084486	1050232	993670	1304901	921522	826598	1203417	998331	1207251	1164390	1184360	976610	1011425	1259821	1066212	1180622	1033899	1015110	1111643	923879	1091746	1147286	899668	927884	993197	1070849	1033934	989561	969102	933262	1024609	1053891	1066645	1083070	1921629	1095983	1220762	1073698	1128952	1038484	79	30	865	872	851	878	937	1204	1311	1209	1755	1899	1877	2462
PG 16:0/18:1	29626	39110	41743	49683	47557	58968	37523	31072	57901	52737	63744	39995	33807	64365	44333	39388	43890	34507	72679	58811	94167	65773	60729	79295	98001	60135	60762	107761	73981	80581	93197	124940	116898	62222	74073	129663	104493	81786	91441	74106	31668	66882	69915	65485	22	38	57601	61738	60268	63408	60709	67404	64255	69735	67974	70067	74013	73205
PG 16:1/16:0	1611	2239	3194	2856	2754	3673	2163	1809	3777	2071	3884	2311	2633	7469	5436	2769	2503	2591	10872	6575	14448	9164	4400	5734	10052	3638	7056	12252	6866	5020	10501	8501	14296	5997	8153	9193	7514	7798	8558	6121	3497	7452	5128	4799	17	16	6002	5675	5785	5790	5572	6017	6289	6446	6124	7661	6964	6666
PG 18:0/16:0	1974	2575	2059	1551	2889	3423	2567	2021	4789	2522	4394	1887	2246	2365	1790	1649	1495	1219	2797	2762	3292	2850	6417	8082	6888	6215	4989	9266	5900	7162	6226	11437	8225	4338	4779	9456	8576	4807	5488	6488	1512	2652	5240	4186	58	19	4068	3965	4131	4346	3866	4146	4478	4544	4503	4982	4093	3859
PG 18:1/16:1	2362	2358	2561	2666	2543	2964	2320	1840	2414	2352	2137	2708	2182	3495	2770	2195	2242	1645	3844	3680	5794	3632	3581	3760	5327	2307	4454	4545	3602	3732	4965	3879	5235	2437	2861	3933	4343	4299	3566	2325	2670	2884	2876	3176	45	65	3139	3026	3514	3065	3723	3824	3542	3259	3044	3831	3101	3122
PG 18:1/18:0	10769	12846	12005	16100	13924	19042	12025	9261	20307	12091	19876	15024	11934	10370	9271	8562	11807	8357	14975	12747	17134	13953	34314	41567	39600	39142	27134	44926	25764	36897	37663	62001	44318	25787	30690	66098	49835	29919	35601	31369	12918	25023	31061	29104	26	40	25465	23869	23581	25728	23883	24542	25553	27976	25723	24855	26207	26721
PG 18:1/18:1	3576	4846	6899	4467	4919	5655	4001	4147	7794	4787	6285	16733	5854	17343	7568	6656	15196	11956	12169	11120	15220	10938	14450	21850	28803	13394	21946	39918	23022	19232	28371	28078	29609	16242	17001	33687	28274	23389	24510	18444	7480	17245	18368	15494	13	17	15608	15474	15367	16671	16238	15569	16672	17757	17074	16479	18226	18310
PG 18:2/16:0	19135	25822	24518	40825	28808	39388	22387	20419	39112	28301	41341	19895	14465	24428	19465	14431	23466	13075	25901	21477	32854	25259	66699	97085	112767	75024	70325	124156	65876	92505	102392	118896	106869	71555	97454	131579	109676	88419	93213	81431	45555	99522	78023	70338	30	53	58985	59646	60360	60378	63595	61428	61810	65477	63487	69526	71726	73168
PG 18:2/18:0	25490	24963	23473	36465	31219	43474	23296	20502	39105	23481	36783	15173	15978	12214	14057	12893	19366	11165	21293	17635	21771	19380	74890	103855	113539	87031	67422	118094	62757	98963	94317	143419	93359	63293	91599	166645	128055	88040	103429	86903	48671	94772	80910	79146	35	64	63204	60135	63642	61596	62516	66118	67364	67581	67866	64697	68032	71452
PG 18:2/18:1	3794	4948	7191	4870	4788	5564	4311	5974	7834	6340	7137	8430	3971	7049	5470	3039	7634	5231	5844	5518	6563	5707	16117	23388	32620	14711	21782	36426	19721	22368	32127	29877	25261	18517	23671	37493	31666	25873	26536	21293	15214	26918	19737	17193	38	34	16608	16096	16259	16184	16818	17745	17306	18616	17491	19287	18911	21548
PG 20:4/16:0	3775	4330	5409	5038	3925	7694	2833	2841	5824	3559	5698	2603	2825	1764	2668	2729	4289	1339	3864	2009	3422	2401	9639	11273	18650	10000	13860	24450	10522	9329	15255	15121	15660	5604	5812	11207	8780	14260	9035	7460	5154	10321	7722	6008	31	28	8316	8153	7808	8214	9054	8536	8369	8528	8390	10330	9404	10388
PG 20:4/16:1	2491	2695	1236	2425	2027	1236	2223	2879	1762	2060	4478	1011	536	750	934	529	579	501	663	1370	793	1280	8580	6801	6915	6526	6667	8174	5834	5101	5505	9228	8194	4299	3376	5930	4936	3795	4398	3299	3361	3179	4298	1501	4	55	3677	3455	3008	4238	4244	3940	3915	4408	3810	3657	4250	3678
PI 15:0/18:1(d7)	11368	10765	13245	14076	9859	8221	10737	12458	12100	13305	12304	9686	12980	12431	10351	9283	11438	12200	7939	12781	8202	9782	9752	10480	12471	12693	8258	9001	10239	9110	11149	9471	8585	13093	9041	6965	10842	10204	16614	11816	11719	7999	11753	11515	47	52	68	110	91	113	56	50	213	156	60	120	75	88
PI 32:1	11098	14489	13814	16113	19619	16711	15687	16620	14250	16521	18292	23945	21176	18903	14942	21331	17858	13838	24892	15962	26339	22907	11347	6193	8586	8051	4934	9705	8727	11143	6430	8545	13463	6818	7626	6800	9498	5790	7648	9023	5635	5250	5875	6622	51	525	11556	8052	7948	7423	13811	10319	9563	12860	11045	11829	9665	14122
PI 32:2	34170	43135	34329	43109	55773	34933	39448	28814	36372	35859	38716	65767	56660	82604	73812	139060	54263	67858	120018	91876	164782	113577	15780	13896	18416	15100	13898	14511	16492	21419	18084	23464	26646	26480	36253	22933	26185	11905	26007	24953	15278	16577	14967	17488	126	734	39356	36704	36299	35841	37888	41223	39314	43755	47712	50559	49127	49362
PI 32:3	4629	3648	4992	5319	5499	6732	6070	4966	5989	6729	3161	12482	8644	13247	11676	17384	7607	9924	16486	14788	20333	19995	2278	2552	2556	2384	3027	1681	1661	3813	1902	2551	3071	4389	6443	3907	2776	2524	3141	3267	4190	4181	1515	2516	288	516	4021	5055	4532	5798	3645	7185	5593	5879	6711	7063	8473	6157
PI 34:0	34949	36818	27710	40675	42668	42000	32706	35888	36157	41368	38680	58438	45839	60082	39811	74185	36349	37075	60806	48819	75147	59212	25727	15699	20687	16093	17956	18589	16783	26245	17134	21122	24944	23969	28103	21997	23378	14047	18593	26980	14774	13929	13885	17495	266	482	24785	29849	25363	30434	23428	26130	25276	30553	31895	32479	31798	39885
PI 34:1	318009	350901	347799	464128	498877	471183	359326	294583	353565	396208	369958	639928	506773	687630	476527	842407	482491	437818	740571	584166	878734	646584	239010	180983	196361	222137	136712	179782	157827	254229	175562	209552	229739	204070	274069	216077	231971	120331	253619	193778	141257	175068	151742	228608	417	365	265985	249950	257738	308787	293635	300018	289068	297529	378903	384908	326247	365132
PI 34:1p	7990	11612	10328	11371	11632	15012	10976	10755	12416	12603	11685	37580	17308	22274	16613	24928	24343	22977	17628	15883	18712	19998	6592	5082	4727	5205	6035	5550	4174	7696	4268	4692	5123	6929	9470	4626	5039	3905	6757	5393	5006	5184	3307	6628	388	819	9719	7637	8984	7562	8940	8506	8821	9218	10827	12879	11718	10771
PI 34:2	740841	832437	597806	995483	949307	914811	725239	675099	726602	871784	831520	1032377	841849	1003754	832127	1067195	759509	764556	1033479	831978	1080602	1020019	289753	258849	292161	332971	205139	271288	258585	426863	290504	363804	355888	411330	613123	388403	418869	262790	460942	340643	313500	320197	289861	427063	1681	656	444038	449404	499205	473695	515136	475918	544230	636780	625260	604752	716612	669651
PI 34:3	22076	32316	21751	40416	41490	41681	31461	29809	37389	38250	32464	52444	35802	49010	43875	52874	33294	41482	55501	47545	59054	55812	15705	11674	16468	14483	9824	9383	11144	12562	10613	11176	15225	18875	29870	20017	15650	13910	16318	19261	16401	14693	11783	14740	536	439	19882	18097	18548	22068	17884	21698	20216	25811	25248	24888	24085	24582
PI 36:0	23658	27456	31639	39418	38407	41803	30704	19440	30056	27940	25439	35864	28708	25527	20395	27431	27521	21603	29923	27389	37605	33518	22073	16786	16633	15452	13172	16732	17322	19251	14264	19075	22848	15257	21506	16143	18058	9460	17365	15070	9464	10247	10480	14170	642	1416	16378	19473	14798	18026	17163	17432	18171	21276	20660	20252	20393	21752
PI 36:1	348461	380663	388197	506096	538379	522305	386750	320399	445014	441860	336103	591647	440760	418639	311332	419824	346347	304411	442237	418066	455105	449591	268216	190248	220890	197142	161190	206721	175571	269451	181630	228768	257454	221268	270977	230870	236455	108892	225306	180283	132743	136830	135380	159224	148	725	214762	249815	231814	239286	266791	231314	267581	267932	271645	279765	298528	289105
PI 36:2	1204212	1249124	1245630	1453051	1366182	1629426	1280771	1019408	1192712	1415023	1218047	2003361	1300624	1563775	1088275	1510739	1352341	1225063	1364151	1418085	1717416	1529934	872999	628380	793031	707227	535057	642025	701324	1010326	658179	792441	770566	911258	1072160	865651	943461	486682	886158	756884	594459	614509	630711	836613	816	706	774323	752784	846925	842486	873868	826638	880092	956470	972096	1070660	1019009	974354
PI 36:3	744842	829233	674900	822039	824381	857818	832407	750668	878016	904617	1001993	1320954	893821	1074780	883563	1179651	1013367	1081411	1002121	1016958	915922	1000625	408202	332610	400679	429603	379707	328063	358222	447466	390000	427009	550664	599461	712674	545086	585065	427496	618158	581010	514111	489781	469975	636869	1244	781	621133	581828	588794	608435	573248	577208	658046	695902	636554	657042	729749	717689
PI 36:4	1378138	1430862	1193932	1830815	1745639	1517274	1685150	1373467	1652027	1465152	1816659	981993	1083892	1170044	1071246	986739	784713	988369	1159656	1154514	1245130	1368057	1011029	865728	965017	986141	1069905	892547	815154	928476	826282	891062	1237254	849051	986978	1301181	1238146	1139287	1119432	1179243	1077971	970237	971276	1005668	1186	493	1021860	1169098	1093622	1059435	1146053	1168435	1078375	1239515	1310508	1210907	1183028	1242505
PI 38:1	13789	16245	11743	15785	16612	14786	15832	14798	9659	15339	12923	10858	10800	7905	12812	11914	11837	8013	14400	17566	18990	15318	9277	6311	7115	4655	7381	7024	10075	7705	9319	9041	10593	10490	8938	10233	10299	7793	9819	8106	5308	7620	5862	7282	516	1362	7953	11094	8154	7356	6728	9495	10493	10749	11324	15645	11848	13242
PI 38:2	164261	158502	151168	123813	152669	116484	170121	157493	139401	142194	160281	90096	154278	122039	135786	135593	126557	140570	165715	167116	141654	177655	173418	135317	155389	157276	140267	152100	128638	186511	151669	159760	214670	142320	156450	171267	183342	156666	154736	151526	141569	127788	142725	151695	71	1066	145866	151384	144014	146912	148207	147871	157855	164494	165875	166744	156254	169246
PI 38:3	1971189	1763218	1703571	1498955	1706230	1246594	1925931	1833070	1625050	1686430	1713910	853274	1630290	1211140	1364490	1331372	1173660	1395069	1576007	1510146	1558424	1608759	2437322	1966140	2143832	2170754	2101339	2338670	1910860	2494066	2147156	2150398	2715092	1804269	1961352	2264040	2330184	2225390	2064504	1984085	2093403	1759379	1929020	1944728	3046	507	1771997	1822292	1833924	1839151	1845716	1973358	1944615	2178933	1975688	2111237	2060180	2115085
PI 38:4	8359844	7582938	6400874	6765825	7014447	6305082	7208509	7239029	6784932	7380279	7740930	3180037	4932396	3855384	4181789	3583727	3380714	3990414	4653298	4456999	4829603	4832531	12754918	10704637	10800209	11647050	10474900	11369404	8639953	11690402	9787111	10914467	11821503	7281086	7176301	11050453	10007042	10775169	8401200	9129312	10553099	7489722	8295526	8701627	9257	6090	8097484	8034827	8179110	9271183	8525768	8471731	8889909	9598366	8952606	9075286	8760822	9328839
PI 38:5	838106	859641	961110	864108	939512	814778	935360	945288	845393	984236	964694	1486017	1074585	1193914	1176936	1063758	996605	1397347	1060872	1179868	1028287	1317252	811650	659198	703233	755939	890143	753352	803940	828142	757529	744394	1059781	969656	1158148	1277143	1180058	1156563	992233	1097321	1269898	1025509	1108953	1116673	125	485	947898	932809	971331	1073847	1029036	1021453	1052433	1149095	1058778	1104725	1118504	1132137
PI 38:6	114598	148722	118318	169935	172345	135793	147845	128563	151097	162969	202012	278243	220659	253181	240826	225682	206256	226713	227863	215972	227516	250404	108486	100881	103376	104355	108397	118381	96965	111829	113683	100351	141460	116394	149253	142522	155517	124212	130903	103098	108660	113849	132727	131643	393	518	124533	134770	134449	141783	142294	140498	152182	152144	146163	147561	151237	162161
PI 40:3	20069	20017	18093	16235	18719	15079	21409	19012	20181	18188	21219	11210	15938	14279	17290	14553	15528	18415	16995	17899	15775	19233	13690	15310	17984	16044	16472	12554	13031	16462	16672	16413	18627	14697	14160	14983	17303	14961	15711	17905	13238	12201	16001	14801	315	298	15625	15623	14759	15397	18411	18610	16519	17565	8468	19483	18007	18329
PI 40:4	80890	82717	77074	77427	88723	60723	91664	79148	88319	92224	85021	42220	54265	47492	60984	38973	42716	51459	58102	54823	56834	65029	92652	77071	92851	89551	91417	86174	70104	89666	88079	88444	103634	69698	67782	78643	94719	74823	75335	75465	70910	68239	77135	76654	400	692	65533	74345	69004	72288	70042	75200	79936	77861	74119	78811	77045	83402
PI 40:5	125665	143884	129495	98378	121642	79201	132211	137102	123747	119270	130135	102130	122364	118430	123449	105928	106533	125451	118037	114549	125515	118842	112407	106579	121785	125287	129171	128921	106826	128755	129561	120326	136341	102012	128305	125832	140400	113739	126874	105165	115888	104889	116421	121745	309	542	106432	112701	110636	116612	121438	122075	120528	134938	120145	126741	126944	123758
PI 40:6	247015	252201	231693	217833	259297	183481	248437	301597	205512	273129	287108	266891	328791	308267	311406	247328	271182	286532	288852	278020	331897	282781	212640	198321	216329	222428	205230	245645	198730	249547	235602	237888	252913	233942	273396	254020	275104	234866	238355	181805	205924	199982	227742	240428	108	617	222394	228904	232155	226626	229709	249217	254360	252461	246948	252304	260026	272936
Sa1P d 18:0	2457	1694	1632	1700	1856	1885	2131	1670	1914	1426	1395	3138	4199	2312	3199	2314	3102	4034	2718	2958	2787	3410	7328	4542	6422	4159	7247	6117	4439	4432	6144	2863	6309	3759	2694	6311	7368	9180	4330	3627	3218	4167	4246	3038	46	51	2825	2876	2819	2675	2486	2685	2498	3380	2886	2826	3015	3424
So1P 17:1	28447	28068	17969	30510	27391	35045	21940	27763	20378	25596	23833	33313	35674	27005	29591	21542	37403	32449	22109	24760	23255	27246	25899	18722	23779	21425	28523	17843	24604	23547	30667	15832	17012	23236	16475	20344	20135	28407	30353	17052	18521	28809	19150	17648	1515	670	1096	634	667	552	1384	1837	3329	3446	3305	3936	3325	4144
So1P d 18:1	17869	16523	12539	15903	16953	21686	14746	14425	14401	12291	14462	22367	23475	12406	21846	14487	19266	21570	18895	16967	20818	19776	34279	20856	28521	22042	37397	25352	25414	22277	29187	17264	27584	18526	13715	27406	28808	38131	17679	17690	16829	23494	18607	15697	649	246	19676	19108	17122	15340	14930	14145	16866	17779	18717	18333	18595	18487
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
PE 32:0
PE 32:1
PE 32:2
PE 34:1
PE 34:1 NEG
PE 34:2
PE 34:3
PE 36:0
PE 36:1
PE 36:1 NEG
PE 36:2
PE 36:3
PE 36:4
PE 36:5
PE 38:0
PE 38:1
PE 38:2
PE 38:3
PE 38:4
PE 38:5
PE 38:6
PE 40:0
PE 40:5
PE 40:6
PE 40:7
PE(O-34:0)
PE(O-34:1)
PE(O-34:2)
PE(O-36:0)
PE(O-36:1)
PE(O-36:2)
PE(O-36:3)
PE(O-36:4)
PE(O-36:5)
PE(O-38:0)
PE(O-38:1)
PE(O-38:2)
PE(O-38:3)
PE(O-38:4)
PE(O-38:5)
PE(O-40:5)
PE(O-40:6)
PE(O-40:7)
PE(P-32:0)
PE(P-34:0)
PE(P-34:1)
PE(P-34:2)
PE(P-36:0)
PE(P-36:1)
PE(P-36:2)
PE(P-36:3)
PE(P-36:4)
PE(P-38:0)
PE(P-38:1)
PE(P-38:2)
PE(P-38:3)
PE(P-38:4)
PE(P-38:5)
PE(P-38:6)
PE(P-40:5)
PE(P-40:6)
PE 15:0/18:1(d7)
PG 15:0/18:1(d7)
PG 16:0/18:1
PG 16:1/16:0
PG 18:0/16:0
PG 18:1/16:1
PG 18:1/18:0
PG 18:1/18:1
PG 18:2/16:0
PG 18:2/18:0
PG 18:2/18:1
PG 20:4/16:0
PG 20:4/16:1
PI 15:0/18:1(d7)
PI 32:1
PI 32:2
PI 32:3
PI 34:0
PI 34:1
PI 34:1p
PI 34:2
PI 34:3
PI 36:0
PI 36:1
PI 36:2
PI 36:3
PI 36:4
PI 38:1
PI 38:2
PI 38:3
PI 38:4
PI 38:5
PI 38:6
PI 40:3
PI 40:4
PI 40:5
PI 40:6
Sa1P d 18:0
So1P 17:1
So1P d 18:1
METABOLITES_END
#END