#METABOLOMICS WORKBENCH tgallagher_20200423_115556 DATATRACK_ID:1991 STUDY_ID:ST001365 ANALYSIS_ID:AN002272 PROJECT_ID:PR000933 VERSION 1 CREATED_ON April 27, 2020, 9:52 am #PROJECT PR:PROJECT_TITLE Antibiotics in Cystic Fibrosis Sputum PR:PROJECT_SUMMARY Antibiotics have enormous impact on microbial communities, yet many studies PR:PROJECT_SUMMARY focused on microbiome and health are confounded by limited information about PR:PROJECT_SUMMARY antibiotic exposure. Given that antibiotics have diverse pharmacokinetic and PR:PROJECT_SUMMARY antibacterial properties, investigating the type, concentration and location PR:PROJECT_SUMMARY would help us understand what microbes encounter at an infection site. Here, we PR:PROJECT_SUMMARY developed Liquid Chromatography Mass Spectrometry (LC-MS) methods to detect 18 PR:PROJECT_SUMMARY antibiotics in clinical samples using cystic fibrosis sputum as an application. PR:PROJECT_SUMMARY Three liquid extraction methods were compared with spike-in controls of PR:PROJECT_SUMMARY antibiotics on the Waters Acquity Quattro Premier XE. Extraction with PR:PROJECT_SUMMARY dithiothreitol captured the most antibiotics and was then used to detect PR:PROJECT_SUMMARY antibiotics in sputum samples from 11 people with cystic fibrosis in comparison PR:PROJECT_SUMMARY to self-reported antibiotic use. For the sputum samples, the Quattro Premier XE PR:PROJECT_SUMMARY method detected nanomolar or micromolar concentrations of 16 antibiotics. In 71 PR:PROJECT_SUMMARY of the 158 sputum samples, at least one antibiotic that was not reported by the PR:PROJECT_SUMMARY subject was detected by the LC-MS method, a discordance largely explained by PR:PROJECT_SUMMARY alternating usage and the long half-life of azithromycin. For ~37% of samples, PR:PROJECT_SUMMARY antibiotics were marked by the subject but were not detected, which has PR:PROJECT_SUMMARY implications for in vivo antibiotic penetrance and efficacy. Our study takes the PR:PROJECT_SUMMARY first step in detecting antibiotics at the infection site, with future goals of PR:PROJECT_SUMMARY incorporating accurate antibiotic data into microbiome studies. PR:INSTITUTE University of California, Irvine PR:DEPARTMENT Molecular Biology & Biochemistry PR:LABORATORY Katrine Whiteson PR:LAST_NAME Gallagher PR:FIRST_NAME Tara PR:ADDRESS 3315 McGaugh Hall, UC IRVINE, Irvine, CA, 92697, USA PR:EMAIL tgallagh@uci.edu PR:PHONE 9498243509 #STUDY ST:STUDY_TITLE Antibiotics in Cystic Fibrosis Sputum ST:STUDY_TYPE Clinical ST:STUDY_SUMMARY Detection of 18 antibiotics in 171 cystic fibrosis sputum samples from the Phase ST:STUDY_SUMMARY 1 Clinical Study. ST:INSTITUTE University of California, Irvine ST:LAST_NAME Gallagher ST:FIRST_NAME Tara ST:ADDRESS 3315 McGaugh Hall, UC IRVINE, Irvine, CA, 92697, USA ST:EMAIL tgallagh@uci.edu ST:PHONE 9498243509 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:AGE_OR_AGE_RANGE 21-56 SU:GENDER Male and female SU:HUMAN_INCLUSION_CRITERIA Cystic Fibrosis, adults #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - WaterBlank1 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-1 SUBJECT_SAMPLE_FACTORS - WaterBlank2 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-2 SUBJECT_SAMPLE_FACTORS - Sample_12270 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-3 SUBJECT_SAMPLE_FACTORS - WaterBlank1a Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-4 SUBJECT_SAMPLE_FACTORS - WaterBlank2a Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-5 SUBJECT_SAMPLE_FACTORS - Water_ABS_0 Factor:Standard RAW_FILE_NAME=tgallagh_phase1_100918-6 SUBJECT_SAMPLE_FACTORS - Water_ABS_140 Factor:Standard RAW_FILE_NAME=tgallagh_phase1_100918-7 SUBJECT_SAMPLE_FACTORS - Water_ABS_410 Factor:Standard RAW_FILE_NAME=tgallagh_phase1_100918-8 SUBJECT_SAMPLE_FACTORS - Water_ABS_1230 Factor:Standard RAW_FILE_NAME=tgallagh_phase1_100918-9 SUBJECT_SAMPLE_FACTORS - Water_ABS_3700 Factor:Standard RAW_FILE_NAME=tgallagh_phase1_100918-10 SUBJECT_SAMPLE_FACTORS - Water_ABS_1000 Factor:Standard RAW_FILE_NAME=tgallagh_phase1_100918-11 SUBJECT_SAMPLE_FACTORS - Water_ABS_3000 Factor:Standard RAW_FILE_NAME=tgallagh_phase1_100918-12 SUBJECT_SAMPLE_FACTORS - Water_ABS_10000 Factor:Standard RAW_FILE_NAME=tgallagh_phase1_100918-13 SUBJECT_SAMPLE_FACTORS - WaterBlank1b Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-14 SUBJECT_SAMPLE_FACTORS - WaterBlank2b Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-15 SUBJECT_SAMPLE_FACTORS - WaterBlank3b Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-16 SUBJECT_SAMPLE_FACTORS - 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Sample_5548 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-28 SUBJECT_SAMPLE_FACTORS - Sample_5552 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-29 SUBJECT_SAMPLE_FACTORS - Sample_13359 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-30 SUBJECT_SAMPLE_FACTORS - WaterBlank4 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-31 SUBJECT_SAMPLE_FACTORS - WaterBlank5 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-32 SUBJECT_SAMPLE_FACTORS - Sample_13285 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-33 SUBJECT_SAMPLE_FACTORS - Sample_1033 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-34 SUBJECT_SAMPLE_FACTORS - Sample_1142 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-35 SUBJECT_SAMPLE_FACTORS - Sample_9080 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-36 SUBJECT_SAMPLE_FACTORS - Sample_1162 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-37 SUBJECT_SAMPLE_FACTORS - Sample_4157 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-38 SUBJECT_SAMPLE_FACTORS - Sample_1035 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-39 SUBJECT_SAMPLE_FACTORS - Sample_5306 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-40 SUBJECT_SAMPLE_FACTORS - Sample_8329 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-41 SUBJECT_SAMPLE_FACTORS - Sample_9283 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-42 SUBJECT_SAMPLE_FACTORS - WaterBlank6 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-43 SUBJECT_SAMPLE_FACTORS - WaterBlank7 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-44 SUBJECT_SAMPLE_FACTORS - Sample_6512 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-45 SUBJECT_SAMPLE_FACTORS - Sample_9248 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-46 SUBJECT_SAMPLE_FACTORS - Sample_6510 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-47 SUBJECT_SAMPLE_FACTORS - Sample_9082 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-48 SUBJECT_SAMPLE_FACTORS - Sample_1056 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-49 SUBJECT_SAMPLE_FACTORS - Sample_13358 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-50 SUBJECT_SAMPLE_FACTORS - Sample_13333 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-51 SUBJECT_SAMPLE_FACTORS - Sample_4137 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-52 SUBJECT_SAMPLE_FACTORS - Sample_5110 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-53 SUBJECT_SAMPLE_FACTORS - Sample_11159 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-54 SUBJECT_SAMPLE_FACTORS - WaterBlank8 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-55 SUBJECT_SAMPLE_FACTORS - WaterBlank9 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-56 SUBJECT_SAMPLE_FACTORS - Sample_8308 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-57 SUBJECT_SAMPLE_FACTORS - Sample_8306 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-58 SUBJECT_SAMPLE_FACTORS - Sample_10138 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-59 SUBJECT_SAMPLE_FACTORS - Sample_1058 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-60 SUBJECT_SAMPLE_FACTORS - Sample_5571 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-61 SUBJECT_SAMPLE_FACTORS - Sample_5112 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-62 SUBJECT_SAMPLE_FACTORS - WaterBlank10 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-63 SUBJECT_SAMPLE_FACTORS - QC1_10uMPOOL Factor:QC_pool RAW_FILE_NAME=tgallagh_phase1_100918-64 SUBJECT_SAMPLE_FACTORS - WaterBlank11 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-65 SUBJECT_SAMPLE_FACTORS - WaterBlank12 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-66 SUBJECT_SAMPLE_FACTORS - Sample_15155 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-67 SUBJECT_SAMPLE_FACTORS - Sample_5334 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-68 SUBJECT_SAMPLE_FACTORS - Sample_5135 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-69 SUBJECT_SAMPLE_FACTORS - Sample_13334 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-70 SUBJECT_SAMPLE_FACTORS - Sample_9246 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-71 SUBJECT_SAMPLE_FACTORS - Sample_4136 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-72 SUBJECT_SAMPLE_FACTORS - Sample_11157 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-73 SUBJECT_SAMPLE_FACTORS - Sample_15138 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-74 SUBJECT_SAMPLE_FACTORS - Sample_11160 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-75 SUBJECT_SAMPLE_FACTORS - Sample_5137 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-76 SUBJECT_SAMPLE_FACTORS - WaterBlank14 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-77 SUBJECT_SAMPLE_FACTORS - WaterBlank15 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-78 SUBJECT_SAMPLE_FACTORS - Sample_1363 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-79 SUBJECT_SAMPLE_FACTORS - Sample_6489 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-80 SUBJECT_SAMPLE_FACTORS - Sample_9251 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-81 SUBJECT_SAMPLE_FACTORS - Sample_9042 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-82 SUBJECT_SAMPLE_FACTORS - Sample_1055 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-83 SUBJECT_SAMPLE_FACTORS - Sample_1059 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-84 SUBJECT_SAMPLE_FACTORS - Sample_6495 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-85 SUBJECT_SAMPLE_FACTORS - Sample_12293 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-86 SUBJECT_SAMPLE_FACTORS - Sample_12296 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-87 SUBJECT_SAMPLE_FACTORS - Sample_13284 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-88 SUBJECT_SAMPLE_FACTORS - WaterBlank16 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-89 SUBJECT_SAMPLE_FACTORS - WaterBlank17 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-90 SUBJECT_SAMPLE_FACTORS - Sample_1360 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-91 SUBJECT_SAMPLE_FACTORS - Sample_9083 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-92 SUBJECT_SAMPLE_FACTORS - Sample_5305 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-93 SUBJECT_SAMPLE_FACTORS - Sample_13354 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-94 SUBJECT_SAMPLE_FACTORS - Sample_11181 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-95 SUBJECT_SAMPLE_FACTORS - Sample_8324 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-96 SUBJECT_SAMPLE_FACTORS - Sample_12269 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-97 SUBJECT_SAMPLE_FACTORS - Sample_9081 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-98 SUBJECT_SAMPLE_FACTORS - Sample_10155 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-99 SUBJECT_SAMPLE_FACTORS - Sample_5335 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-100 SUBJECT_SAMPLE_FACTORS - WaterBlank18 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-101 SUBJECT_SAMPLE_FACTORS - WaterBlank19 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-102 SUBJECT_SAMPLE_FACTORS - Sample_15141 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-103 SUBJECT_SAMPLE_FACTORS - Sample_12291 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-104 SUBJECT_SAMPLE_FACTORS - Sample_1359 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-105 SUBJECT_SAMPLE_FACTORS - Sample_10139 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-106 SUBJECT_SAMPLE_FACTORS - Sample_6516 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-107 SUBJECT_SAMPLE_FACTORS - Sample_9084 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-108 SUBJECT_SAMPLE_FACTORS - Sample_5550 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-109 SUBJECT_SAMPLE_FACTORS - Sample_6493 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-110 SUBJECT_SAMPLE_FACTORS - Sample_9041 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-111 SUBJECT_SAMPLE_FACTORS - Sample_15159 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-112 SUBJECT_SAMPLE_FACTORS - WaterBlank20 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-113 SUBJECT_SAMPLE_FACTORS - WaterBlank21 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-114 SUBJECT_SAMPLE_FACTORS - Sample_13332 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-115 SUBJECT_SAMPLE_FACTORS - Sample_15136 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-116 SUBJECT_SAMPLE_FACTORS - Sample_1361 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-117 SUBJECT_SAMPLE_FACTORS - Sample_4135 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-118 SUBJECT_SAMPLE_FACTORS - Sample_8305 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-119 SUBJECT_SAMPLE_FACTORS - WaterBlank22 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-120 SUBJECT_SAMPLE_FACTORS - QC2_10uMPOOL Factor:QC_pool RAW_FILE_NAME=tgallagh_phase1_100918-121 SUBJECT_SAMPLE_FACTORS - WaterBlank23 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-122 SUBJECT_SAMPLE_FACTORS - WaterBlank24 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-123 SUBJECT_SAMPLE_FACTORS - WaterBlank25 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-124 SUBJECT_SAMPLE_FACTORS - Sample_4159 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-125 SUBJECT_SAMPLE_FACTORS - Sample_6491 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-126 SUBJECT_SAMPLE_FACTORS - Sample_5574 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-127 SUBJECT_SAMPLE_FACTORS - Sample_1158 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-128 SUBJECT_SAMPLE_FACTORS - Sample_5333 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-129 SUBJECT_SAMPLE_FACTORS - Sample_11180 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-130 SUBJECT_SAMPLE_FACTORS - Sample_13357 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-131 SUBJECT_SAMPLE_FACTORS - Sample_15140 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-132 SUBJECT_SAMPLE_FACTORS - Sample_15134 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-133 SUBJECT_SAMPLE_FACTORS - Sample_8304 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-134 SUBJECT_SAMPLE_FACTORS - WaterBlank26 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-135 SUBJECT_SAMPLE_FACTORS - WaterBlank27 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-136 SUBJECT_SAMPLE_FACTORS - Sample_11185 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-137 SUBJECT_SAMPLE_FACTORS - Sample_15158 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-138 SUBJECT_SAMPLE_FACTORS - Sample_13288 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-139 SUBJECT_SAMPLE_FACTORS - Sample_1034 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-140 SUBJECT_SAMPLE_FACTORS - Sample_9284 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-141 SUBJECT_SAMPLE_FACTORS - Sample_4139 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-142 SUBJECT_SAMPLE_FACTORS - Sample_1159 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-143 SUBJECT_SAMPLE_FACTORS - Sample_5336 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-144 SUBJECT_SAMPLE_FACTORS - Sample_1160 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-145 SUBJECT_SAMPLE_FACTORS - Sample_1362 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-146 SUBJECT_SAMPLE_FACTORS - WaterBlank28 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-147 SUBJECT_SAMPLE_FACTORS - WaterBlank29 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-148 SUBJECT_SAMPLE_FACTORS - Sample_8330 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-149 SUBJECT_SAMPLE_FACTORS - Sample_11186 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-150 SUBJECT_SAMPLE_FACTORS - Sample_5572 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-151 SUBJECT_SAMPLE_FACTORS - Sample_13283 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-152 SUBJECT_SAMPLE_FACTORS - Sample_8331 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-153 SUBJECT_SAMPLE_FACTORS - Sample_1038 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-154 SUBJECT_SAMPLE_FACTORS - Sample_6496 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-155 SUBJECT_SAMPLE_FACTORS - Sample_5551 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-156 SUBJECT_SAMPLE_FACTORS - Sample_13356 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-157 SUBJECT_SAMPLE_FACTORS - Sample_5549 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-158 SUBJECT_SAMPLE_FACTORS - WaterBlank30 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-159 SUBJECT_SAMPLE_FACTORS - WaterBlank31 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-160 SUBJECT_SAMPLE_FACTORS - Sample_12267 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-161 SUBJECT_SAMPLE_FACTORS - Sample_9282 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-162 SUBJECT_SAMPLE_FACTORS - Sample_1161 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-163 SUBJECT_SAMPLE_FACTORS - Sample_6490 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-164 SUBJECT_SAMPLE_FACTORS - Sample_1054 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-165 SUBJECT_SAMPLE_FACTORS - Sample_15162 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-166 SUBJECT_SAMPLE_FACTORS - Sample_5113 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-167 SUBJECT_SAMPLE_FACTORS - Sample_4155 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-168 SUBJECT_SAMPLE_FACTORS - Sample_9043 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-169 SUBJECT_SAMPLE_FACTORS - Sample_1140 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-170 SUBJECT_SAMPLE_FACTORS - WaterBlank32 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-171 SUBJECT_SAMPLE_FACTORS - WaterBlank33 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-172 SUBJECT_SAMPLE_FACTORS - Sample_5136 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-173 SUBJECT_SAMPLE_FACTORS - Sample_5570 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-174 SUBJECT_SAMPLE_FACTORS - Sample_8325 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-175 SUBJECT_SAMPLE_FACTORS - Sample_11162 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-176 SUBJECT_SAMPLE_FACTORS - WaterBlank34 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-177 SUBJECT_SAMPLE_FACTORS - QC3_10uMPOOL Factor:QC_pool RAW_FILE_NAME=tgallagh_phase1_100918-178 SUBJECT_SAMPLE_FACTORS - WaterBlank35 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-179 SUBJECT_SAMPLE_FACTORS - WaterBlank36 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-180 SUBJECT_SAMPLE_FACTORS - Sample_4140 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-181 SUBJECT_SAMPLE_FACTORS - Sample_11187 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-182 SUBJECT_SAMPLE_FACTORS - Sample_9250 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-183 SUBJECT_SAMPLE_FACTORS - Sample_12294 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-184 SUBJECT_SAMPLE_FACTORS - Sample_6515 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-185 SUBJECT_SAMPLE_FACTORS - Sample_4138 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-186 SUBJECT_SAMPLE_FACTORS - Sample_13355 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-187 SUBJECT_SAMPLE_FACTORS - Sample_9285 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-188 SUBJECT_SAMPLE_FACTORS - Sample_6494 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-189 SUBJECT_SAMPLE_FACTORS - Sample_5332 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-190 SUBJECT_SAMPLE_FACTORS - WaterBlank37 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-191 SUBJECT_SAMPLE_FACTORS - WaterBlank38 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-192 SUBJECT_SAMPLE_FACTORS - Sample_5138 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-193 SUBJECT_SAMPLE_FACTORS - Sample_9247 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-194 SUBJECT_SAMPLE_FACTORS - Sample_10159 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-195 SUBJECT_SAMPLE_FACTORS - Sample_13286 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-196 SUBJECT_SAMPLE_FACTORS - Sample_4156 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-197 SUBJECT_SAMPLE_FACTORS - Sample_6513 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-198 SUBJECT_SAMPLE_FACTORS - Sample_9040 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-199 SUBJECT_SAMPLE_FACTORS - Sample_11182 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-200 SUBJECT_SAMPLE_FACTORS - Sample_9038 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-201 SUBJECT_SAMPLE_FACTORS - Sample_1310 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-202 SUBJECT_SAMPLE_FACTORS - WaterBlank39 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-203 SUBJECT_SAMPLE_FACTORS - WaterBlank40 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-204 SUBJECT_SAMPLE_FACTORS - Sample_4158 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-205 SUBJECT_SAMPLE_FACTORS - Sample_10141 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-206 SUBJECT_SAMPLE_FACTORS - Sample_8303 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-207 SUBJECT_SAMPLE_FACTORS - Sample_5575 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-208 SUBJECT_SAMPLE_FACTORS - Sample_5111 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-209 SUBJECT_SAMPLE_FACTORS - Sample_12292 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-210 SUBJECT_SAMPLE_FACTORS - Sample_9286 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-211 SUBJECT_SAMPLE_FACTORS - Sample_11158 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-212 SUBJECT_SAMPLE_FACTORS - Sample_1137 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-213 SUBJECT_SAMPLE_FACTORS - Sample_8327 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-214 SUBJECT_SAMPLE_FACTORS - WaterBlank41 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-215 SUBJECT_SAMPLE_FACTORS - WaterBlank42 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-216 SUBJECT_SAMPLE_FACTORS - Sample_9287 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-217 SUBJECT_SAMPLE_FACTORS - Sample_13287 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-218 SUBJECT_SAMPLE_FACTORS - Sample_1138 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-219 SUBJECT_SAMPLE_FACTORS - Sample_15137 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-220 SUBJECT_SAMPLE_FACTORS - Sample_1141 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-221 SUBJECT_SAMPLE_FACTORS - Sample_8307 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-222 SUBJECT_SAMPLE_FACTORS - Sample_11161 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-223 SUBJECT_SAMPLE_FACTORS - Sample_9249 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-224 SUBJECT_SAMPLE_FACTORS - Sample_12295 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-225 SUBJECT_SAMPLE_FACTORS - Sample_5547 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-226 SUBJECT_SAMPLE_FACTORS - WaterBlank43 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-227 SUBJECT_SAMPLE_FACTORS - WaterBlank44 Factor:Blank RAW_FILE_NAME=tgallagh_phase1_100918-228 SUBJECT_SAMPLE_FACTORS - Sample_9085 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-229 SUBJECT_SAMPLE_FACTORS - Sample_13309 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-230 SUBJECT_SAMPLE_FACTORS - Sample_4154 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-231 SUBJECT_SAMPLE_FACTORS - Sample_6492 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-232 SUBJECT_SAMPLE_FACTORS - Sample_1036 Factor:CF_sample RAW_FILE_NAME=tgallagh_phase1_100918-233 #COLLECTION CO:COLLECTION_SUMMARY Sputum samples from 11 subjects with cystic fibrosis were selected from a larger CO:COLLECTION_SUMMARY airway microbiome study that was approved by the University of Michigan Medical CO:COLLECTION_SUMMARY School Institutional Review Board. Subjects were 6 males and 5 females, age 21 CO:COLLECTION_SUMMARY to 56 years (median 37). Sputum samples were collected by subjects at home and CO:COLLECTION_SUMMARY stored at 4˚C for up to 23 days and then transferred to -80˚C. CO:SAMPLE_TYPE Sputum CO:STORAGE_CONDITIONS Described in summary #TREATMENT TR:TREATMENT_SUMMARY The goal of this study was to determine which antibiotics are present in cystic TR:TREATMENT_SUMMARY fibrosis sputum using a LC-MS method. There were no experimental conditions. The TR:TREATMENT_SUMMARY types of samples included in the LC-MS run were: cystic fibrosis sputum, TR:TREATMENT_SUMMARY external standard curve, quality control pools of external standards run every TR:TREATMENT_SUMMARY ~50 samples, and washes (blanks). #SAMPLEPREP SP:SAMPLEPREP_SUMMARY The extraction solvent was chilled 1% DTT and spiked with 1.33 µM of both SP:SAMPLEPREP_SUMMARY internal standards, linezolid-d3 and levofloxacin-d8. Solvent (150 µL) was SP:SAMPLEPREP_SUMMARY added to 50 µL of sputum. The samples were vortexed for 30 seconds, shaken at SP:SAMPLEPREP_SUMMARY 4˚C on a shaking platform with moderate agitation for 15 minutes, and SP:SAMPLEPREP_SUMMARY centrifuged at 13,200 RCF for 10 minutes at 4˚C. The supernatant was pipetted SP:SAMPLEPREP_SUMMARY into amber glass vials and injected directly into the LC-MS. SP:PROCESSING_STORAGE_CONDITIONS On ice SP:EXTRACT_STORAGE On ice #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Waters Acquity I-Class CH:COLUMN_NAME Waters Acquity BEH C18 (50 x 2.1mm, 1.7 um) CH:FLOW_RATE 0.3 ml/min CH:COLUMN_TEMPERATURE 50 CH:METHODS_FILENAME qopt_A2B2_3g_c9_5min.wvhp CH:SOLVENT_A 0.1% v/v formic acid and 2 mM ammonium acetate in water CH:SOLVENT_B d 0.1% v/v formic acid and 2 mM ammonium acetate in methanol CH:INJECTION_TEMPERATURE 10 #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE CF_antibiotics_quant.mdb AN:ACQUISITION_PARAMETERS_FILE CF_antibiotics.exp #MS MS:INSTRUMENT_NAME Waters Quattro Premier XE MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS The retention times (RT) and MS/MS parameters for reach antibiotic were MS:MS_COMMENTS determined using the QuanOpt function in the Waters Masslynx software. Peaks MS:MS_COMMENTS were automatically picked and filtered in QuanLynx. Downstream filtering was MS:MS_COMMENTS conducted in R. Data were imported into R and the following criteria for MS:MS_COMMENTS filtering were applied: minimum peak area under the curve of 20 and signal to MS:MS_COMMENTS noise ratio of 10. Because several of the antibiotics had high carry-over rates MS:MS_COMMENTS (including ciprofloxacin, levofloxacin, and trimethoprim), sample peaks were MS:MS_COMMENTS also filtered out when the AUC was lower than the wash ran directly before the MS:MS_COMMENTS sample. Scripts can be found at the following github link: MS:MS_COMMENTS https://github.com/tgallagh/LCMS_Antibiotics. MS:CAPILLARY_VOLTAGE 3.3 kV MS:COLLISION_GAS Nitrogen MS:SOURCE_TEMPERATURE 125 MS:DESOLVATION_GAS_FLOW 800 L/h MS:DESOLVATION_TEMPERATURE 400 #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS micromolar MS_METABOLITE_DATA_START Samples Sample_10138 Sample_10139 Sample_10141 Sample_10155 Sample_10159 Sample_1033 Sample_1034 Sample_1035 Sample_1036 Sample_1038 Sample_1054 Sample_1055 Sample_1056 Sample_1058 Sample_1059 Sample_11157 Sample_11158 Sample_11159 Sample_11160 Sample_11161 Sample_11162 Sample_11180 Sample_11181 Sample_11182 Sample_11185 Sample_11186 Sample_11187 Sample_1137 Sample_1138 Sample_1140 Sample_1141 Sample_1142 Sample_1158 Sample_1159 Sample_1160 Sample_1161 Sample_1162 Sample_1163 Sample_12267 Sample_12269 Sample_12270 Sample_12291 Sample_12292 Sample_12293 Sample_12294 Sample_12295 Sample_12296 Sample_1310 Sample_13283 Sample_13284 Sample_13285 Sample_13286 Sample_13287 Sample_13288 Sample_13309 Sample_13332 Sample_13333 Sample_13334 Sample_13354 Sample_13355 Sample_13356 Sample_13357 Sample_13358 Sample_13359 Sample_1359 Sample_1360 Sample_1361 Sample_1362 Sample_1363 Sample_15134 Sample_15136 Sample_15137 Sample_15138 Sample_15139 Sample_15140 Sample_15141 Sample_15155 Sample_15158 Sample_15159 Sample_15162 Sample_4135 Sample_4136 Sample_4137 Sample_4138 Sample_4139 Sample_4140 Sample_4154 Sample_4155 Sample_4156 Sample_4157 Sample_4158 Sample_4159 Sample_5110 Sample_5111 Sample_5112 Sample_5113 Sample_5135 Sample_5136 Sample_5137 Sample_5138 Sample_5305 Sample_5306 Sample_5307 Sample_5331 Sample_5332 Sample_5333 Sample_5334 Sample_5335 Sample_5336 Sample_5547 Sample_5548 Sample_5549 Sample_5550 Sample_5551 Sample_5552 Sample_5570 Sample_5571 Sample_5572 Sample_5573 Sample_5574 Sample_5575 Sample_6489 Sample_6490 Sample_6491 Sample_6492 Sample_6493 Sample_6494 Sample_6495 Sample_6496 Sample_6510 Sample_6512 Sample_6513 Sample_6515 Sample_6516 Sample_6517 Sample_8303 Sample_8304 Sample_8305 Sample_8306 Sample_8307 Sample_8308 Sample_8324 Sample_8325 Sample_8327 Sample_8329 Sample_8330 Sample_8331 Sample_9038 Sample_9039 Sample_9040 Sample_9041 Sample_9042 Sample_9043 Sample_9080 Sample_9081 Sample_9082 Sample_9083 Sample_9084 Sample_9085 Sample_9246 Sample_9247 Sample_9248 Sample_9249 Sample_9250 Sample_9251 Sample_9282 Sample_9283 Sample_9284 Sample_9285 Sample_9286 Sample_9287 Water_ABS_0 Water_ABS_1000 Water_ABS_10000 Water_ABS_1230 Water_ABS_140 Water_ABS_3000 Water_ABS_3700 Water_ABS_410 WaterBlank1 WaterBlank10 WaterBlank11 WaterBlank12 WaterBlank14 WaterBlank15 WaterBlank16 WaterBlank17 WaterBlank18 WaterBlank19 WaterBlank1a WaterBlank1A WaterBlank1b WaterBlank2 WaterBlank20 WaterBlank21 WaterBlank22 WaterBlank23 WaterBlank24 WaterBlank25 WaterBlank26 WaterBlank27 WaterBlank28 WaterBlank29 WaterBlank2a WaterBlank2A WaterBlank2b WaterBlank30 WaterBlank31 WaterBlank32 WaterBlank33 WaterBlank34 WaterBlank35 WaterBlank36 WaterBlank37 WaterBlank38 WaterBlank39 WaterBlank3A WaterBlank3b WaterBlank4 WaterBlank40 WaterBlank41 WaterBlank42 WaterBlank43 WaterBlank44 WaterBlank5 WaterBlank6 WaterBlank7 WaterBlank8 WaterBlank9 Minus80aliquot QC1_10uMPOOL QC2_10uMPOOL QC3_10uMPOOL Factors Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:CF_sample Factor:Standard Factor:Standard Factor:Standard Factor:Standard Factor:Standard Factor:Standard Factor:Standard Factor:Standard Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Blank Factor:Storage_QC Factor:QC_pool Factor:QC_pool Factor:QC_pool Levofloxacind 0.39 0.20 0.04 0.19 0.08 0.00 0.15 0.21 0.07 0.23 0.10 0.12 0.32 0.17 0.13 0.33 0.09 0.29 0.26 0.14 0.18 0.14 0.25 0.06 0.16 0.13 0.09 0.05 0.05 0.13 0.07 0.09 0.18 0.12 0.00 0.11 0.10 0.04 0.26 0.43 0.18 0.47 0.07 0.60 0.19 0.14 0.28 0.06 0.14 0.23 0.12 0.09 0.11 0.11 0.10 0.16 0.23 0.23 0.27 0.12 0.11 0.26 0.14 0.41 0.13 0.21 0.09 0.00 0.13 0.17 0.14 0.07 0.29 0.07 0.29 0.12 0.24 0.16 0.15 0.13 0.60 0.31 0.43 0.18 0.22 0.16 0.08 0.14 0.08 0.20 0.09 0.24 0.64 0.09 0.79 0.18 0.84 0.20 0.38 0.28 0.36 0.25 0.16 0.29 0.23 0.35 0.34 0.59 0.52 0.14 0.44 0.44 0.52 0.52 0.48 0.22 0.67 0.28 0.15 0.37 0.09 0.24 0.19 0.12 0.00 0.15 0.09 0.23 0.14 0.45 0.32 0.12 0.11 0.16 0.09 0.07 0.46 0.18 0.27 0.13 0.31 0.23 0.10 0.07 0.14 0.24 0.33 0.08 0.14 0.12 0.27 0.38 0.36 0.16 0.69 0.51 0.27 0.54 0.12 0.38 0.12 0.35 0.13 0.15 0.32 0.39 0.33 0.51 0.14 0.07 0.14 1.29 0.57 0.17 1.43 1.30 0.39 1.51 1.33 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Linezolidd 0.22 0.00 0.01 0.17 0.06 0.00 0.11 0.04 0.10 0.11 0.05 0.06 0.21 0.27 0.11 0.15 0.04 0.23 0.13 0.07 0.03 0.07 0.21 0.00 0.06 0.16 0.03 0.01 0.04 0.04 0.03 0.05 0.06 0.13 0.00 0.05 0.08 0.06 0.09 0.31 0.35 0.44 0.03 0.16 0.05 0.12 0.18 0.03 0.05 0.13 0.07 0.05 0.05 0.07 0.09 0.08 0.32 0.28 0.18 0.03 0.09 0.08 0.16 0.09 0.28 0.09 0.09 0.00 0.15 0.09 0.06 0.03 0.10 0.10 0.10 0.17 0.30 0.11 0.07 0.05 0.19 0.15 0.31 0.02 0.13 0.02 0.06 0.03 0.02 0.06 0.03 0.10 0.38 0.02 0.53 0.06 0.54 0.05 0.47 0.05 0.16 0.07 0.14 0.15 0.04 0.09 0.32 0.36 0.26 0.07 0.10 0.10 0.00 0.13 0.10 0.04 0.45 0.15 0.14 0.15 0.03 0.09 0.04 0.03 0.05 0.08 0.01 0.11 0.03 0.11 0.04 0.07 0.02 0.29 0.11 0.03 0.15 0.11 0.30 0.05 0.24 0.15 0.02 0.02 0.03 0.16 0.12 0.02 0.09 0.12 0.21 0.23 0.14 0.07 0.38 0.13 0.15 0.00 0.10 0.17 0.05 0.12 0.11 0.03 0.17 0.12 0.09 0.22 0.02 0.03 0.09 1.03 1.08 0.64 1.10 1.10 0.92 1.06 1.07 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Amikacin 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.99 10.07 0.32 0.00 2.28 1.46 0.00 2.00 0.00 13.08 4.15 0.00 5.53 2.64 0.85 0.00 0.00 4.69 0.00 0.00 0.00 0.00 0.00 1.68 4.45 0.00 0.00 1.68 3.74 9.67 8.20 5.56 0.00 0.00 0.00 0.00 0.00 0.00 1.68 17.67 0.00 1.62 14.78 4.11 18.88 6.09 0.00 15.07 0.00 0.00 0.00 6.04 0.00 4.75 2.88 0.00 0.00 154.23 136.81 119.09 118.52 Amoxicillin 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.40 10.01 0.13 0.02 3.17 0.18 0.02 0.00 0.06 0.00 0.56 0.00 0.48 0.00 0.00 0.00 0.38 0.00 4.69 0.00 7.13 1.76 0.00 0.00 0.00 0.00 2.09 0.44 0.00 0.00 0.00 0.44 8.41 0.00 0.00 0.00 0.00 0.00 0.00 0.37 3.87 0.00 0.00 0.00 4.67 0.37 0.35 0.00 0.73 0.00 0.00 0.00 0.53 0.00 0.12 0.00 0.41 355.82 257.94 260.17 0.00 Ampicillin 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.35 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.26 10.00 0.09 0.02 3.27 0.23 0.04 0.00 0.00 0.00 1.61 0.00 0.00 0.00 0.00 0.10 0.82 0.17 8.73 0.00 8.62 1.10 0.09 0.13 0.00 2.95 5.41 0.00 0.00 3.45 0.00 0.46 6.74 3.18 0.00 0.00 0.00 0.00 0.86 0.00 4.69 0.00 0.00 3.33 5.89 0.00 0.21 2.53 0.45 0.00 0.00 0.00 0.05 0.00 0.00 0.49 0.00 331.74 935.97 619.89 0.00 Azithromycin 158.68 0.00 492.64 113.35 109.29 0.00 290.84 1876.61 196.90 211.91 522.11 546.35 81.47 202.56 235.30 86.16 35.98 116.92 96.34 109.42 293.98 679.96 272.33 0.00 533.71 246.31 313.99 964.97 767.42 560.40 2367.47 442.17 1009.00 483.16 0.00 595.49 271.40 232.85 38.39 29.26 11.98 16.85 41.54 50.32 50.53 18.68 64.55 185.85 262.49 148.51 68.04 76.46 164.93 231.99 267.68 134.36 120.16 188.03 357.76 341.20 255.28 414.06 0.00 81.42 156.64 840.48 756.54 0.00 234.49 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 22.57 76.94 96.61 221.39 121.29 164.39 82.24 193.69 77.69 80.19 27.50 93.57 27.15 17.69 9.07 30.95 6.82 43.31 28.49 55.07 10.31 13.71 6.71 3.53 16.25 92.17 12.55 5.03 4.86 37.08 16.42 24.01 0.00 9.32 9.17 26.74 8.91 11.23 4.93 17.29 12.72 376.99 392.69 1921.64 270.51 337.10 444.55 311.24 909.19 603.39 2096.00 60.66 731.82 259.25 113.74 72.47 205.71 776.63 258.05 1015.80 353.02 571.11 515.37 282.92 3386.77 683.47 420.96 242.87 35.15 23.40 25.70 40.16 15.48 41.44 21.40 228.60 129.95 0.00 67.11 145.85 45.23 89.54 42.86 235.00 57.70 29.41 128.44 22.97 130.84 51.81 36.16 0.00 1.04 9.73 0.29 0.01 3.54 0.32 0.01 17.66 3.32 292.84 1.77 0.00 11.49 46.29 4.70 21.53 14.01 0.00 0.00 0.55 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2.15 45.82 523.93 148.47 6.03 0.00 0.00 7.30 0.00 7.00 0.00 0.00 159.01 235.73 0.00 86.09 14.12 177.46 10.46 0.00 0.00 5.20 0.00 6.18 0.00 0.00 28.70 8.89 6.20 27.00 2417.31 10702.12 11331.24 14909.98 Aztreonam 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 4.97 15.13 9.86 0.00 16.86 5.99 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.46 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.89 1.66 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 82.95 0.00 13.41 19.58 34.59 0.00 18.20 17.19 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.28 29.21 9.34 81.67 8.16 25.50 0.00 0.00 0.00 0.00 0.00 0.00 27.22 3.33 26.35 0.00 0.00 30.53 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.21 10.00 0.08 0.03 3.30 0.22 0.04 0.00 0.00 2.64 3.12 0.00 0.29 0.41 0.54 0.00 0.00 0.15 5.91 0.00 0.00 11.41 0.83 0.00 0.00 0.00 1.03 0.00 0.00 0.13 0.06 0.15 6.29 0.00 0.00 0.00 0.00 0.00 0.88 13.01 2.85 4.60 0.00 1.79 0.82 0.52 0.00 0.00 0.00 0.00 0.16 0.37 0.37 0.00 0.00 0.00 0.00 325.06 275.13 288.22 0.00 Cefepime 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.44 9.99 0.00 0.00 3.47 0.00 0.25 0.00 0.00 0.00 0.00 0.00 0.00 3.63 9.72 0.00 9.85 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 38.18 0.00 0.00 0.00 0.00 0.00 0.00 0.00 14.84 0.00 19.00 0.00 0.00 0.00 40.77 0.00 0.00 6.68 0.00 0.00 10.14 7.23 70.85 36.12 0.00 13.10 0.00 2.16 0.00 2.68 0.00 0.00 277.63 279.60 327.33 0.00 Ceftazidime 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 20.84 15.28 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 9.31 30.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.90 9.99 0.24 0.00 3.38 0.00 0.00 0.00 2.40 0.00 2.00 0.43 0.00 2.07 1.14 1.05 0.00 0.00 2.39 0.00 16.36 0.00 2.46 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2.74 0.00 0.00 0.00 0.00 0.00 0.00 0.00 8.14 0.00 2.65 2.04 0.00 0.00 0.00 4.70 0.00 4.76 0.00 0.00 8.80 0.77 0.00 4.03 2.69 1.51 361.18 217.57 218.94 0.00 Ceftriaxone 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 3.90 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 3.46 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.02 1.19 10.00 0.09 0.02 3.32 0.09 0.02 0.00 0.00 37.88 14.97 0.00 0.00 0.00 0.00 3.64 0.00 0.00 34.53 0.00 20.96 5.41 0.00 0.00 0.00 30.24 0.00 0.00 0.00 2.77 0.00 0.00 17.31 0.78 0.00 0.59 2.75 0.00 0.00 59.29 28.76 2.95 0.00 11.76 0.00 7.83 0.00 0.00 0.00 0.00 3.18 0.00 1.39 0.54 1.55 1.54 1.03 326.50 288.48 288.60 0.00 Ciprofloxacin 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 6.22 6.35 3.08 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 6.78 7.12 5.62 5.77 8.71 4.96 0.00 0.00 0.00 8.27 6.03 13.35 0.00 0.00 0.00 0.00 0.00 0.00 0.00 3.32 0.00 0.00 0.00 0.00 0.00 0.00 6.13 4.95 6.57 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 6.57 5.62 5.75 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 5.93 2.79 5.11 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.39 1.67 10.03 0.57 0.41 2.91 0.73 0.46 0.00 0.00 9.43 8.50 1.10 1.65 0.49 1.17 3.53 1.50 1.04 11.69 0.00 28.84 0.92 1.10 2.02 0.00 6.34 8.39 1.59 0.00 4.44 2.48 2.05 11.95 5.41 0.00 0.00 0.69 0.00 0.80 9.42 0.00 0.00 0.00 0.75 8.71 4.79 1.99 1.79 0.00 0.00 0.50 1.76 2.31 1.12 2.19 0.00 0.00 375.23 408.15 450.16 0.00 Levofloxacin 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 6.41 8.90 12.84 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.46 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.51 10.02 0.14 0.00 3.04 0.34 0.04 0.00 0.00 6.25 2.87 0.00 0.00 0.05 0.21 0.35 0.00 0.00 9.38 0.00 7.71 0.36 0.09 0.15 0.00 0.00 0.00 0.19 0.00 0.48 0.89 0.06 10.21 3.55 0.00 0.21 0.10 0.00 0.00 9.98 6.61 0.01 0.00 0.25 8.23 2.69 0.00 0.00 0.00 0.00 0.03 0.62 0.00 0.00 1.14 0.28 1.20 324.96 450.55 364.59 0.00 Linezolid 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 126.30 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.80 10.71 0.11 0.02 2.97 0.30 0.04 11.04 1.00 0.00 11.65 0.00 1.49 0.12 0.02 0.00 0.00 0.00 468.68 4.41 0.00 0.00 1.78 0.14 56.39 4.20 0.00 0.24 0.00 1.32 0.00 0.54 0.00 0.00 0.04 0.00 0.04 0.07 0.03 183.33 40.05 0.00 0.00 0.05 475.96 1.84 0.00 1.70 0.00 0.00 0.00 0.14 19.58 2.02 7.64 0.00 12.77 4928.66 4667.76 2137.16 2190.31 Meropenem 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.48 10.08 0.09 0.02 2.90 0.36 0.03 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Piperacillin 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 3.53 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.74 10.03 1.20 1.18 2.90 1.25 1.19 0.00 1.17 6.67 1.88 1.19 1.20 0.00 1.28 0.00 0.00 0.00 8.00 0.00 0.00 1.42 0.00 1.21 0.00 1.69 0.00 0.00 0.00 1.26 1.42 0.00 7.69 3.77 0.00 1.18 1.29 0.00 1.25 28.35 0.00 1.21 1.61 1.51 1.72 2.11 1.44 2.22 0.00 0.00 1.25 0.00 1.26 0.00 1.30 0.00 0.00 225.20 151.16 138.08 0.00 Sulfamethoxazole 0.00 0.00 0.00 0.00 4.16 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 20.95 23.53 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 11.75 12.89 4.99 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 1.19 9.99 0.08 0.02 3.35 0.22 0.03 0.00 0.44 0.00 2.93 0.00 0.08 0.00 1.13 0.10 0.00 0.00 7.59 0.00 2.30 0.53 0.00 0.19 0.00 0.00 0.00 0.08 0.00 0.08 0.14 0.00 10.02 0.49 0.00 0.00 0.06 0.00 0.01 5.21 0.00 0.00 0.00 1.67 4.32 0.43 0.27 0.00 0.00 0.00 0.08 0.48 0.74 0.20 0.11 0.00 0.40 340.45 398.16 337.58 0.00 Tobramycin 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 19.88 14.10 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 9.18 30.13 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.93 9.99 0.08 0.02 3.39 0.33 0.06 0.00 0.00 0.00 0.00 0.33 0.00 0.00 0.99 1.41 0.00 0.00 0.16 0.00 0.00 0.00 1.28 0.35 0.00 0.00 1.79 0.96 0.00 0.56 1.17 0.00 2.14 0.13 0.00 1.68 0.00 0.00 0.55 0.00 4.26 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.28 0.00 0.00 0.00 0.98 0.00 0.00 0.14 0.00 316.32 218.77 216.82 0.00 Trimethoprim 0.00 0.00 0.00 0.00 13.50 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 12.86 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 29.93 25.05 11.49 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.67 10.04 0.36 0.00 2.86 0.60 0.15 0.00 0.01 2.26 1.78 0.09 0.00 0.67 0.44 0.00 0.00 0.00 6.56 0.00 3.89 0.00 0.00 0.00 0.00 0.00 0.00 0.22 0.00 0.59 0.00 0.05 7.63 0.00 0.00 0.00 0.00 0.00 0.37 6.20 0.00 0.00 0.00 0.00 0.00 3.00 0.00 1.54 0.00 0.00 0.00 2.24 0.00 1.41 2.83 0.00 0.38 273.69 358.39 369.91 0.00 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name m/z Retention time (min) PubChem Levofloxacind 371.1 2.93 46782054 Linezolidd 341.1 3.34 25217322 Amikacin 585.98 0.39 37768 Amoxicillin 365.98 1.08 33613 Ampicillin 350 3.14 6249 Azithromycin 749.25 3.44 447043 Aztreonam 435.94 2.61 5742832 Cefepime 480.99 0.81 5479537 Ceftazidime 546.91 2.08 5481173 Ceftriaxone 554.85 2.95 5479530 Ciprofloxacin 332.08 3.04 2764 Levofloxacin 362.07 2.93 149096 Linezolid 338.1 3.34 441401 Meropenem 384.07 2.34 441130 Piperacillin 518.04 3.46 43672 Sulfamethoxazole 254.03 3.04 5329 Tobramycin 467.96 2.08 36294 Trimethoprim 291.11 2.89 5578 METABOLITES_END #END