#METABOLOMICS WORKBENCH epannkuk_20200601_070355_mwtab.txt DATATRACK_ID:2033 STUDY_ID:ST001387 ANALYSIS_ID:AN002315 PROJECT_ID:PR000951
VERSION             	1
CREATED_ON             	June 2, 2020, 10:47 am
#PROJECT
PR:PROJECT_TITLE                 	Bat liver lipid profiles
PR:PROJECT_SUMMARY               	In this study, we investigated changes in hepatic lipid profiles of little brown
PR:PROJECT_SUMMARY               	bats (Myotis lucifugus) and big brown bats (Eptesicus fuscus) at an early stage
PR:PROJECT_SUMMARY               	(70 d) of infection with the etiological agent, Pseudogymnoascus destructans
PR:PROJECT_SUMMARY               	(Pd).
PR:INSTITUTE                     	Georgetown University
PR:LAST_NAME                     	Pannkuk
PR:FIRST_NAME                    	Evan
PR:ADDRESS                       	3970 Reservoir Rd, NW New Research Build, washington dc, District of Columbia,
PR:ADDRESS                       	20057, USA
PR:EMAIL                         	elp44@georgetown.edu
PR:PHONE                         	2026875650
#STUDY
ST:STUDY_TITLE                   	Bat liver lipid profiles
ST:STUDY_SUMMARY                 	In this study, we investigated changes in hepatic lipid profiles of little brown
ST:STUDY_SUMMARY                 	bats (Myotis lucifugus) and big brown bats (Eptesicus fuscus) at an early stage
ST:STUDY_SUMMARY                 	(70 d) of infection with the etiological agent, Pseudogymnoascus destructans
ST:STUDY_SUMMARY                 	(Pd).
ST:INSTITUTE                     	Georgetown University
ST:LAST_NAME                     	Pannkuk
ST:FIRST_NAME                    	Evan
ST:ADDRESS                       	3970 Reservoir Rd, NW New Research Build, washington dc, District of Columbia,
ST:ADDRESS                       	20057, USA
ST:EMAIL                         	elp44@georgetown.edu
ST:PHONE                         	2026875650
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Myotis lucifugus
SU:TAXONOMY_ID                   	59463
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_10	Treatment:Pd	Sex=f; RAW_FILE_NAME=POS_10.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_11	Treatment:Pd	Sex=f; RAW_FILE_NAME=POS_11.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_12	Treatment:Pd	Sex=f; RAW_FILE_NAME=POS_12.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_13	Treatment:Pd	Sex=f; RAW_FILE_NAME=POS_13.raw
SUBJECT_SAMPLE_FACTORS           	EPFU	POS_14	Treatment:sham	Sex=f; RAW_FILE_NAME=POS_14.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_15	Treatment:sham	Sex=f; RAW_FILE_NAME=POS_15.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_16	Treatment:sham	Sex=m; RAW_FILE_NAME=POS_16.raw
SUBJECT_SAMPLE_FACTORS           	EPFU	POS_17	Treatment:sham	Sex=m; RAW_FILE_NAME=POS_17.raw
SUBJECT_SAMPLE_FACTORS           	EPFU	POS_18	Treatment:Pd	Sex=m; RAW_FILE_NAME=POS_18.raw
SUBJECT_SAMPLE_FACTORS           	EPFU	POS_19	Treatment:sham	Sex=f; RAW_FILE_NAME=POS_19.raw
SUBJECT_SAMPLE_FACTORS           	EPFU	POS_20	Treatment:sham	Sex=f; RAW_FILE_NAME=POS_20.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_21	Treatment:sham	Sex=f; RAW_FILE_NAME=POS_21.raw
SUBJECT_SAMPLE_FACTORS           	EPFU	POS_22	Treatment:sham	Sex=m; RAW_FILE_NAME=POS_22.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_23	Treatment:Pd	Sex=m; RAW_FILE_NAME=POS_23.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_27	Treatment:sham	Sex=f; RAW_FILE_NAME=POS_27.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_28	Treatment:sham	Sex=m; RAW_FILE_NAME=POS_28.raw
SUBJECT_SAMPLE_FACTORS           	EPFU	POS_29	Treatment:sham	Sex=m; RAW_FILE_NAME=POS_29.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_30	Treatment:Pd	Sex=m; RAW_FILE_NAME=POS_30.raw
SUBJECT_SAMPLE_FACTORS           	EPFU	POS_31	Treatment:sham	Sex=f; RAW_FILE_NAME=POS_31.raw
SUBJECT_SAMPLE_FACTORS           	EPFU	POS_32	Treatment:Pd	Sex=m; RAW_FILE_NAME=POS_32.raw
SUBJECT_SAMPLE_FACTORS           	EPFU	POS_33	Treatment:Pd	Sex=f; RAW_FILE_NAME=POS_33.raw
SUBJECT_SAMPLE_FACTORS           	EPFU	POS_34	Treatment:sham	Sex=m; RAW_FILE_NAME=POS_34.raw
SUBJECT_SAMPLE_FACTORS           	EPFU	POS_35	Treatment:Pd	Sex=f; RAW_FILE_NAME=POS_35.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_36	Treatment:sham	Sex=f; RAW_FILE_NAME=POS_36.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_37	Treatment:Pd	Sex=m; RAW_FILE_NAME=POS_37.raw
SUBJECT_SAMPLE_FACTORS           	EPFU	POS_38	Treatment:Pd	Sex=m; RAW_FILE_NAME=POS_38.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_42	Treatment:Pd	Sex=m; RAW_FILE_NAME=POS_42.raw
SUBJECT_SAMPLE_FACTORS           	EPFU	POS_43	Treatment:Pd	Sex=f; RAW_FILE_NAME=POS_43.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_44	Treatment:sham	Sex=m; RAW_FILE_NAME=POS_44.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_45	Treatment:sham	Sex=f; RAW_FILE_NAME=POS_45.raw
SUBJECT_SAMPLE_FACTORS           	EPFU	POS_46	Treatment:Pd	Sex=m; RAW_FILE_NAME=POS_46.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_47	Treatment:sham	Sex=m; RAW_FILE_NAME=POS_47.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_48	Treatment:Pd	Sex=m; RAW_FILE_NAME=POS_48.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_49	Treatment:sham	Sex=m; RAW_FILE_NAME=POS_49.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_50	Treatment:sham	Sex=f; RAW_FILE_NAME=POS_50.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_51	Treatment:sham	Sex=m; RAW_FILE_NAME=POS_51.raw
SUBJECT_SAMPLE_FACTORS           	MYLU	POS_52	Treatment:Pd	Sex=m; RAW_FILE_NAME=POS_52.raw
SUBJECT_SAMPLE_FACTORS           	EPFU	POS_53	Treatment:Pd	Sex=f; RAW_FILE_NAME=POS_53.raw
#COLLECTION
CO:COLLECTION_SUMMARY            	Laboratory experiments were conducted at the University of Winnipeg (Manitoba
CO:COLLECTION_SUMMARY            	Sustainable Development Species at Risk/Wildlife Scientific Permit # SAR16009).
CO:COLLECTION_SUMMARY            	M. lucifugus were collected from a WNS-negative hibernaculum in Central Manitoba
CO:COLLECTION_SUMMARY            	in January (mid-winter) 2017. E. fuscus were collected from a WNS-negative
CO:COLLECTION_SUMMARY            	hibernaculum in northwestern Ontario near Kenora in the same month. Hibernating
CO:COLLECTION_SUMMARY            	bats were removed from the cave wall by hand and transferred to a bio-secure bat
CO:COLLECTION_SUMMARY            	facility as previously described (Warnecke et al., 2012; McGuire et al., 2016).
CO:COLLECTION_SUMMARY            	Bats were tested to be negative for Pd upon arrival using standard qPCR
CO:COLLECTION_SUMMARY            	techniques and inoculated using previously established protocols (Warnecke et
CO:COLLECTION_SUMMARY            	al., 2012; McGuire et al., 2016).
CO:SAMPLE_TYPE                   	Liver
#TREATMENT
TR:TREATMENT_SUMMARY             	Bats given sham (PBST) or Pd inoculation
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Liver samples (~10 mg) were homogenized with cold methanol (300 μl) containing
SP:SAMPLEPREP_SUMMARY            	internal standards (10 μl SPLASH® Lipidomix®), 10 μl was removed for protein
SP:SAMPLEPREP_SUMMARY            	quantification, and the tissue homogenate was incubated on ice for 5 min.
SP:SAMPLEPREP_SUMMARY            	Chilled chloroform (600 μl) was added to samples and followed by shaking
SP:SAMPLEPREP_SUMMARY            	(vortex 30 sec) and further incubation on ice (10 min). Chilled water (300 μl)
SP:SAMPLEPREP_SUMMARY            	was added to samples followed by shaking (vortex 30 sec) and incubation on ice
SP:SAMPLEPREP_SUMMARY            	(10 min), then centrifuged for 10 min (10,000 x g, 4°C) to separate layers. The
SP:SAMPLEPREP_SUMMARY            	lower organic phase was separated, evaporated under vacuum, and reconstituted in
SP:SAMPLEPREP_SUMMARY            	200 μl isopropanol (IPA):acetonitrile (ACN):H2O (2:1:1) for analysis.
SP:PROCESSING_STORAGE_CONDITIONS 	On ice
SP:EXTRACT_STORAGE               	On ice
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Waters Acquity
CH:COLUMN_NAME                   	Waters Acquity CSH C18 (100 x 2.1mm, 1.7um)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Waters Synapt G2 QTOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	None
MS:MS_RESULTS_FILE               	ST001387_AN002315_Results.txt	UNITS:m/z values	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END