#METABOLOMICS WORKBENCH cclish_20201105_085425 DATATRACK_ID:2228 STUDY_ID:ST001520 ANALYSIS_ID:AN002531
VERSION                          	1
CREATED_ON                       	02-08-2024
#PROJECT
PR:PROJECT_TITLE                 	Role of Diet in the Reconstitution of the Human Gut Microbiome and its
PR:PROJECT_TITLE                 	Metabolome
PR:PROJECT_TYPE                  	Observational study
PR:PROJECT_SUMMARY               	We studied the impact of three divergent diets, vegan, omnivore, and a synthetic
PR:PROJECT_SUMMARY               	enteral nutrition (EEN) diet lacking fiber, on the human gut microbiome and its
PR:PROJECT_SUMMARY               	metabolome in a longitudinal analysis that included a microbiota depletion
PR:PROJECT_SUMMARY               	intervention. Hybrid nontargeted LC-MS methods were used to profile stool and
PR:PROJECT_SUMMARY               	plasma metabolites.
PR:INSTITUTE                     	Broad Institute of MIT and Harvard
PR:LAST_NAME                     	Clish
PR:FIRST_NAME                    	Clary
PR:ADDRESS                       	415 Main Street, Cambridge, MA, 02142, USA
PR:EMAIL                         	clary@broadinstitute.org
PR:PHONE                         	617-714-7654
PR:FUNDING_SOURCE                	Crohn’s & Colitis Foundation, P30 DK 050306, PennCHOP Microbiome Program, and
PR:FUNDING_SOURCE                	the Penn Center for Nutritional Science and Medicine
PR:DOI                           	http://dx.doi.org/10.21228/M8B984
PR:CONTRIBUTORS                  	Ceylan Tanes, Kyle Bittinger, Yuan Gao, Elliot S. Friedman, Lisa Nessel, Unmesha
PR:CONTRIBUTORS                  	Roy Paladhi, Lillian Chau, Erika Panfen, Michael A. Fischbach, Jonathan Braun,
PR:CONTRIBUTORS                  	Ramnik J. Xavier, Clary B. Clish, Hongzhe Li, Frederic D. Bushman, James D.
PR:CONTRIBUTORS                  	Lewis, Gary D. Wu
#STUDY
ST:STUDY_TITLE                   	Stool unknowns profiled using hybrid nontargeted methods (part-II)
ST:STUDY_SUMMARY                 	We determined the effect of diet on the composition and metabolic function of
ST:STUDY_SUMMARY                 	human gut microbiome using a controlled feeding experiment with three divergent
ST:STUDY_SUMMARY                 	diets (vegan, omnivore, and an exclusive enteral nutrition diet (EEN) devoid of
ST:STUDY_SUMMARY                 	dietary fiber) in the Food And Resulting Microbial Metabolites (FARMM) study.
ST:STUDY_SUMMARY                 	The study included an antibiotic and polyethylene glycol (Abx/PEG) intervention
ST:STUDY_SUMMARY                 	to dynamically assess the effect of diet on the reconstitution of the gut
ST:STUDY_SUMMARY                 	microbiota and its associated fecal and plasma metabolome. Samples from thirty
ST:STUDY_SUMMARY                 	healthy volunteers between the ages of 18 and 60, 10 mper diet group were
ST:STUDY_SUMMARY                 	analyzed. Self-reported vegans were required to have followed a vegan diet for a
ST:STUDY_SUMMARY                 	minimum of 6 months prior to enrollment. Key exclusion criteria included
ST:STUDY_SUMMARY                 	inflammatory bowel disease, celiac disease, or other chronic intestinal
ST:STUDY_SUMMARY                 	disorders; prior bowel resection surgery other than appendectomy; baseline bowel
ST:STUDY_SUMMARY                 	frequency less than every 2 days or greater than 3 times daily; creatinine
ST:STUDY_SUMMARY                 	concentration greater than the upper limit of normal; diabetes mellitus;
ST:STUDY_SUMMARY                 	currently smoking; body mass index (BMI) <18.5 or >35; and use of
ST:STUDY_SUMMARY                 	antibiotics and probiotics in the prior 6 months. The 10 vegans continued to
ST:STUDY_SUMMARY                 	follow their usual diet as outpatients. All participants completed the Diet
ST:STUDY_SUMMARY                 	History Questionnaire II (DHQ II), a food frequency questionnaire developed by
ST:STUDY_SUMMARY                 	the Risk Factor Monitoring and Methods Branch of the National Cancer Institute.
ST:STUDY_SUMMARY                 	The vegan participants also completed three 24 hour diet recalls with a
ST:STUDY_SUMMARY                 	dietitian in the week prior to starting antibiotics. We randomly assigned the 20
ST:STUDY_SUMMARY                 	omnivores to receive an omnivore diet or EEN (Modulen® IBD) while residing in
ST:STUDY_SUMMARY                 	an inpatient research unit. The macronutrient composition of Modulen® is
ST:STUDY_SUMMARY                 	protein 36g, fat 47g, and carbohydrate 110g per 1000 Kcal. The two omnivore
ST:STUDY_SUMMARY                 	diets were engineered to have a similar composition to EEN. All the subjects
ST:STUDY_SUMMARY                 	consumed the menu A on day 11 and menu B on days 4 and 14. Diets for omnivores
ST:STUDY_SUMMARY                 	were constructed to provide the expected total calories required per day for the
ST:STUDY_SUMMARY                 	participant to maintain their current weight and were adjusted if there was
ST:STUDY_SUMMARY                 	weight gain or loss of more than 2.5 pounds. On days 6, 7, and 8, inpatients
ST:STUDY_SUMMARY                 	participants received vancomycin 500mg orally every 6 hours and neomycin 1000mg
ST:STUDY_SUMMARY                 	orally every 6 hours. On day 7, participants consumed 4L of polyethylene glycol
ST:STUDY_SUMMARY                 	(PEG) based bowel purgative (GoLytely®). Participants in the omnivore and EEN
ST:STUDY_SUMMARY                 	arms left the inpatient research unit only under the direct supervision of a
ST:STUDY_SUMMARY                 	research staff member. The vegan outpatients reported to the hospital twice on
ST:STUDY_SUMMARY                 	days 6,7 and 8 to receive antibiotics and to consume PEG on day 7. The first
ST:STUDY_SUMMARY                 	stool sample of each day of the inpatient groups was collected, aliquoted and
ST:STUDY_SUMMARY                 	frozen immediately at -80oC. Blood was collected on days 1, 5, 9, 12 and 15 from
ST:STUDY_SUMMARY                 	which plasma aliquots were immediately isolated and frozen at -80oC. The
ST:STUDY_SUMMARY                 	outpatient participants following the vegan diet also had blood collected on
ST:STUDY_SUMMARY                 	days 1, 5, 9, 12, and 15 from which plasma aliquots were immediately isolated
ST:STUDY_SUMMARY                 	and frozen at -80oC. Among these participants, the first stool of the day was
ST:STUDY_SUMMARY                 	collected at home daily and kept on ice packs until it was brought to the
ST:STUDY_SUMMARY                 	research unit where it could be aliquoted and frozen. Samples from vegans were
ST:STUDY_SUMMARY                 	received for aliquoting within 24 hours and on average within 4 hours (Wu et
ST:STUDY_SUMMARY                 	al., 2010). Day 0 stool was not collected from vegans since their diet did not
ST:STUDY_SUMMARY                 	change. The aliquoted amounts for all samples ranged from 500 mg to 1 g. The
ST:STUDY_SUMMARY                 	aliquots were taken from different areas of the sample. Remaining sample was
ST:STUDY_SUMMARY                 	then collected into one residual 50 ml conical tube with a tongue depressor. Any
ST:STUDY_SUMMARY                 	remaining stool was discarded. The University of Pennsylvania Institutional
ST:STUDY_SUMMARY                 	Review Board (IRB) approved the research protocol.
ST:INSTITUTE                     	Broad Institute of MIT and Harvard
ST:LAST_NAME                     	Clish
ST:FIRST_NAME                    	Clary
ST:ADDRESS                       	415 Main Street, Cambridge, MA, 02142, USA
ST:EMAIL                         	clary@broadinstitute.org
ST:PHONE                         	617-714-7654
ST:SUBMIT_DATE                   	2020-11-05
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
SU:AGE_OR_AGE_RANGE              	20-60
SU:WEIGHT_OR_WEIGHT_RANGE        	BMI range: 20-35
SU:GENDER                        	Male and female
SU:HUMAN_RACE                    	White; American Indian/Alaskan Native; Black/African American
SU:HUMAN_ETHNICITY               	Hispanic or Latino; Non-Hispanic or Latino
SU:HUMAN_TRIAL_TYPE              	Intervention trial
SU:HUMAN_EXCLUSION_CRITERIA      	Key exclusion criteria included inflammatory bowel disease, celiac disease, or
SU:HUMAN_EXCLUSION_CRITERIA      	other chronic intestinal disorders; prior bowel resection surgery other than
SU:HUMAN_EXCLUSION_CRITERIA      	appendectomy; baseline bowel frequency less than every 2 days or greater than 3
SU:HUMAN_EXCLUSION_CRITERIA      	times daily; creatinine concentration greater than the upper limit of normal;
SU:HUMAN_EXCLUSION_CRITERIA      	diabetes mellitus; currently smoking; body mass index (BMI) <18.5 or >35;
SU:HUMAN_EXCLUSION_CRITERIA      	and use of antibiotics and probiotics in the prior 6 months
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	9010	9010-1-SE	Study_Diet:Western | Sex:Female | Time:Baseline	Type=Stool; Age=24; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0302_WUG_FARMM_HIL-9010-1-SE.raw 0302_WUG_FARMM_LIP-9010-1-SE.raw 0302_WU_FARMM_CMH_9010-1-SE.raw 0302_WUG_FARMM_FFA-9010-1-SE.raw
SUBJECT_SAMPLE_FACTORS           	9015	9015-1-SE	Study_Diet:Western | Sex:Female | Time:Baseline	Type=Stool; Age=23; BMI=20.5; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0249_WUG_FARMM_HIL-9015-1-SE.raw 0249_WUG_FARMM_LIP-9015-1-SE.raw 0249_WU_FARMM_CMH_9015-1-SE.raw 0249_WUG_FARMM_FFA-9015-1-SE.raw
SUBJECT_SAMPLE_FACTORS           	9005	9005-1-SE	Study_Diet:Western | Sex:Male | Time:Baseline	Type=Stool; Age=23; BMI=21.6; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0261_WUG_FARMM_HIL-9005-1-SE.raw 0261_WUG_FARMM_LIP-9005-1-SE.raw 0261_WU_FARMM_CMH_9005-1-SE.raw 0261_WUG_FARMM_FFA-9005-1-SE.raw
SUBJECT_SAMPLE_FACTORS           	9006	9006-1-SE	Study_Diet:Western | Sex:Male | Time:Baseline	Type=Stool; Age=24; BMI=28.2; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0200_WUG_FARMM_HIL-9006-1-SE.raw 0200_WUG_FARMM_LIP-9006-1-SE.raw 0200_WU_FARMM_CMH_9006-1-SE.raw 0200_WUG_FARMM_FFA-9006-1-SE.raw
SUBJECT_SAMPLE_FACTORS           	9019	9019-1-SE	Study_Diet:Western | Sex:Male | Time:Baseline	Type=Stool; Age=36; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0216_WUG_FARMM_HIL-9019-1-SE.raw 0216_WUG_FARMM_LIP-9019-1-SE.raw 0216_WU_FARMM_CMH_9019-1-SE.raw 0216_WUG_FARMM_FFA-9019-1-SE.raw
SUBJECT_SAMPLE_FACTORS           	9030	9030-1-SE	Study_Diet:Western | Sex:Male | Time:Baseline	Type=Stool; Age=46; BMI=27.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0203_WUG_FARMM_HIL-9030-1-SE.raw 0203_WUG_FARMM_LIP-9030-1-SE.raw 0203_WU_FARMM_CMH_9030-1-SE.raw 0203_WUG_FARMM_FFA-9030-1-SE.raw
SUBJECT_SAMPLE_FACTORS           	9033	9033-1-SE	Study_Diet:Western | Sex:Male | Time:Baseline	Type=Stool; Age=57; BMI=27.1; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0183_WUG_FARMM_HIL-9033-1-SE.raw 0302c_0183re_WUG_FARMM_LIP-9033-1-SE.raw 0183_WU_FARMM_CMH_9033-1-SE.raw 0183_WUG_FARMM_FFA-9033-1-SE.raw
SUBJECT_SAMPLE_FACTORS           	9036	9036-1-SE	Study_Diet:Western | Sex:Male | Time:Baseline	Type=Stool; Age=60; BMI=23.9; Ethnicity=Not Hispanic or Latino; Race=American Indian/Alaskan Native White; RAW_FILE_NAME=0233_WUG_FARMM_HIL-9036-1-SE.raw 0233_WUG_FARMM_LIP-9036-1-SE.raw 0233_WU_FARMM_CMH_9036-1-SE.raw 0233_WUG_FARMM_FFA-9036-1-SE.raw
SUBJECT_SAMPLE_FACTORS           	9037	9037-1-SE	Study_Diet:Western | Sex:Male | Time:Baseline	Type=Stool; Age=29; BMI=23; Ethnicity=Hispanic or Latino; Race=Black/African American White; RAW_FILE_NAME=0275_WUG_FARMM_HIL-9037-1-SE.raw 0275_WUG_FARMM_LIP-9037-1-SE.raw 0275_WU_FARMM_CMH_9037-1-SE.raw 0275_WUG_FARMM_FFA-9037-1-SE.raw
SUBJECT_SAMPLE_FACTORS           	9040	9040-1-SE	Study_Diet:Western | Sex:Male | Time:Baseline	Type=Stool; Age=22; BMI=23.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0269_WUG_FARMM_HIL-9040-1-SE.raw 0302i_0269re_WUG_FARMM_LIP-9040-1-SE.raw 0269_WU_FARMM_CMH_9040-1-SE.raw 0269_WUG_FARMM_FFA-9040-1-SE.raw
SUBJECT_SAMPLE_FACTORS           	9010	9010-1-SD	Study_Diet:Western | Sex:Female | Time:Day 5	Type=Stool; Age=24; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0298_WUG_FARMM_HIL-9010-1-SD.raw 0298_WUG_FARMM_LIP-9010-1-SD.raw 0298_WU_FARMM_CMH_9010-1-SD.raw 0298_WUG_FARMM_FFA-9010-1-SD.raw
SUBJECT_SAMPLE_FACTORS           	9015	9015-1-SD	Study_Diet:Western | Sex:Female | Time:Day 5	Type=Stool; Age=23; BMI=20.5; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0250_WUG_FARMM_HIL-9015-1-SD.raw 0250_WUG_FARMM_LIP-9015-1-SD.raw 0250_WU_FARMM_CMH_9015-1-SD.raw 0250_WUG_FARMM_FFA-9015-1-SD.raw
SUBJECT_SAMPLE_FACTORS           	9005	9005-1-SD	Study_Diet:Western | Sex:Male | Time:Day 5	Type=Stool; Age=23; BMI=21.6; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0258_WUG_FARMM_HIL-9005-1-SD.raw 0258_WUG_FARMM_LIP-9005-1-SD.raw 0258_WU_FARMM_CMH_9005-1-SD.raw 0258_WUG_FARMM_FFA-9005-1-SD.raw
SUBJECT_SAMPLE_FACTORS           	9006	9006-1-SD	Study_Diet:Western | Sex:Male | Time:Day 5	Type=Stool; Age=24; BMI=28.2; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0197_WUG_FARMM_HIL-9006-1-SD.raw 0197_WUG_FARMM_LIP-9006-1-SD.raw 0197_WU_FARMM_CMH_9006-1-SD.raw 0197_WUG_FARMM_FFA-9006-1-SD.raw
SUBJECT_SAMPLE_FACTORS           	9019	9019-1-SD	Study_Diet:Western | Sex:Male | Time:Day 5	Type=Stool; Age=36; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0213_WUG_FARMM_HIL-9019-1-SD.raw 0213_WUG_FARMM_LIP-9019-1-SD.raw 0213_WU_FARMM_CMH_9019-1-SD.raw 0213_WUG_FARMM_FFA-9019-1-SD.raw
SUBJECT_SAMPLE_FACTORS           	9030	9030-1-SD	Study_Diet:Western | Sex:Male | Time:Day 5	Type=Stool; Age=46; BMI=27.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0202_WUG_FARMM_HIL-9030-1-SD.raw 0202_WUG_FARMM_LIP-9030-1-SD.raw 0202_WU_FARMM_CMH_9030-1-SD.raw 0202_WUG_FARMM_FFA-9030-1-SD.raw
SUBJECT_SAMPLE_FACTORS           	9033	9033-1-SD	Study_Diet:Western | Sex:Male | Time:Day 5	Type=Stool; Age=57; BMI=27.1; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0186_WUG_FARMM_HIL-9033-1-SD.raw 0186_WUG_FARMM_LIP-9033-1-SD.raw 0186_WU_FARMM_CMH_9033-1-SD.raw 0186_WUG_FARMM_FFA-9033-1-SD.raw
SUBJECT_SAMPLE_FACTORS           	9036	9036-1-SD	Study_Diet:Western | Sex:Male | Time:Day 5	Type=Stool; Age=60; BMI=23.9; Ethnicity=Not Hispanic or Latino; Race=American Indian/Alaskan Native White; RAW_FILE_NAME=0236_WUG_FARMM_HIL-9036-1-SD.raw 0236_WUG_FARMM_LIP-9036-1-SD.raw 0236_WU_FARMM_CMH_9036-1-SD.raw 0236_WUG_FARMM_FFA-9036-1-SD.raw
SUBJECT_SAMPLE_FACTORS           	9037	9037-1-SD	Study_Diet:Western | Sex:Male | Time:Day 5	Type=Stool; Age=29; BMI=23; Ethnicity=Hispanic or Latino; Race=Black/African American White; RAW_FILE_NAME=0273_WUG_FARMM_HIL-9037-1-SD.raw 0273_WUG_FARMM_LIP-9037-1-SD.raw 0273_WU_FARMM_CMH_9037-1-SD.raw 0273_WUG_FARMM_FFA-9037-1-SD.raw
SUBJECT_SAMPLE_FACTORS           	9040	9040-1-SD	Study_Diet:Western | Sex:Male | Time:Day 5	Type=Stool; Age=22; BMI=23.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0268_WUG_FARMM_HIL-9040-1-SD.raw 0268_WUG_FARMM_LIP-9040-1-SD.raw 0268_WU_FARMM_CMH_9040-1-SD.raw 0268_WUG_FARMM_FFA-9040-1-SD.raw
SUBJECT_SAMPLE_FACTORS           	9010	9010-1-SC	Study_Diet:Western | Sex:Female | Time:Day 9	Type=Stool; Age=24; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0299_WUG_FARMM_HIL-9010-1-SC.raw 0302h_0299ri_WUG_FARMM_LIP-9010-1-SC.raw 0299_WU_FARMM_CMH_9010-1-SC.raw 0299_WUG_FARMM_FFA-9010-1-SC.raw
SUBJECT_SAMPLE_FACTORS           	9015	9015-1-SC	Study_Diet:Western | Sex:Female | Time:Day 9	Type=Stool; Age=23; BMI=20.5; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0251_WUG_FARMM_HIL-9015-1-SC.raw 0251_WUG_FARMM_LIP-9015-1-SC.raw 0251_WU_FARMM_CMH_9015-1-SC.raw 0251_WUG_FARMM_FFA-9015-1-SC.raw
SUBJECT_SAMPLE_FACTORS           	9005	9005-1-SC	Study_Diet:Western | Sex:Male | Time:Day 9	Type=Stool; Age=23; BMI=21.6; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0259_WUG_FARMM_HIL-9005-1-SC.raw 0259_WUG_FARMM_LIP-9005-1-SC.raw 0259_WU_FARMM_CMH_9005-1-SC.raw 0259_WUG_FARMM_FFA-9005-1-SC.raw
SUBJECT_SAMPLE_FACTORS           	9006	9006-1-SC	Study_Diet:Western | Sex:Male | Time:Day 9	Type=Stool; Age=24; BMI=28.2; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0201_WUG_FARMM_HIL-9006-1-SC.raw 0201_WUG_FARMM_LIP-9006-1-SC.raw 0201_WU_FARMM_CMH_9006-1-SC.raw 0201_WUG_FARMM_FFA-9006-1-SC.raw
SUBJECT_SAMPLE_FACTORS           	9019	9019-1-SC	Study_Diet:Western | Sex:Male | Time:Day 9	Type=Stool; Age=36; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0215_WUG_FARMM_HIL-9019-1-SC.raw 0302g_0215re_WUG_FARMM_LIP-9019-1-SC.raw 0215_WU_FARMM_CMH_9019-1-SC.raw 0215_WUG_FARMM_FFA-9019-1-SC.raw
SUBJECT_SAMPLE_FACTORS           	9030	9030-1-SC	Study_Diet:Western | Sex:Male | Time:Day 9	Type=Stool; Age=46; BMI=27.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0206_WUG_FARMM_HIL-9030-1-SC.raw 0206_WUG_FARMM_LIP-9030-1-SC.raw 0206_WU_FARMM_CMH_9030-1-SC.raw 0206_WUG_FARMM_FFA-9030-1-SC.raw
SUBJECT_SAMPLE_FACTORS           	9033	9033-1-SC	Study_Diet:Western | Sex:Male | Time:Day 9	Type=Stool; Age=57; BMI=27.1; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0185_WUG_FARMM_HIL-9033-1-SC.raw 0185_WUG_FARMM_LIP-9033-1-SC.raw 0185_WU_FARMM_CMH_9033-1-SC.raw 0185_WUG_FARMM_FFA-9033-1-SC.raw
SUBJECT_SAMPLE_FACTORS           	9036	9036-1-SC	Study_Diet:Western | Sex:Male | Time:Day 9	Type=Stool; Age=60; BMI=23.9; Ethnicity=Not Hispanic or Latino; Race=American Indian/Alaskan Native White; RAW_FILE_NAME=0235_WUG_FARMM_HIL-9036-1-SC.raw 0235_WUG_FARMM_LIP-9036-1-SC.raw 0235_WU_FARMM_CMH_9036-1-SC.raw 0235_WUG_FARMM_FFA-9036-1-SC.raw
SUBJECT_SAMPLE_FACTORS           	9037	9037-1-SC	Study_Diet:Western | Sex:Male | Time:Day 9	Type=Stool; Age=29; BMI=23; Ethnicity=Hispanic or Latino; Race=Black/African American White; RAW_FILE_NAME=0272_WUG_FARMM_HIL-9037-1-SC.raw 0272_WUG_FARMM_LIP-9037-1-SC.raw 0272_WU_FARMM_CMH_9037-1-SC.raw 0272_WUG_FARMM_FFA-9037-1-SC.raw
SUBJECT_SAMPLE_FACTORS           	9040	9040-1-SC	Study_Diet:Western | Sex:Male | Time:Day 9	Type=Stool; Age=22; BMI=23.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0270_WUG_FARMM_HIL-9040-1-SC.raw 0270_WUG_FARMM_LIP-9040-1-SC.raw 0270_WU_FARMM_CMH_9040-1-SC.raw 0270_WUG_FARMM_FFA-9040-1-SC.raw
SUBJECT_SAMPLE_FACTORS           	9010	9010-1-SB	Study_Diet:Western | Sex:Female | Time:Day 12	Type=Stool; Age=24; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0300_WUG_FARMM_HIL-9010-1-SB.raw 0300_WUG_FARMM_LIP-9010-1-SB.raw 0300_WU_FARMM_CMH_9010-1-SB.raw 0300_WUG_FARMM_FFA-9010-1-SB.raw
SUBJECT_SAMPLE_FACTORS           	9015	9015-1-SB	Study_Diet:Western | Sex:Female | Time:Day 12	Type=Stool; Age=23; BMI=20.5; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0247_WUG_FARMM_HIL-9015-1-SB.raw 0247_WUG_FARMM_LIP-9015-1-SB.raw 0247_WU_FARMM_CMH_9015-1-SB.raw 0247_WUG_FARMM_FFA-9015-1-SB.raw
SUBJECT_SAMPLE_FACTORS           	9005	9005-1-SB	Study_Diet:Western | Sex:Male | Time:Day 12	Type=Stool; Age=23; BMI=21.6; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0257_WUG_FARMM_HIL-9005-1-SB.raw 0257_WUG_FARMM_LIP-9005-1-SB.raw 0257_WU_FARMM_CMH_9005-1-SB.raw 0257_WUG_FARMM_FFA-9005-1-SB.raw
SUBJECT_SAMPLE_FACTORS           	9006	9006-1-SB	Study_Diet:Western | Sex:Male | Time:Day 12	Type=Stool; Age=24; BMI=28.2; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0199_WUG_FARMM_HIL-9006-1-SB.raw 0199_WUG_FARMM_LIP-9006-1-SB.raw 0199_WU_FARMM_CMH_9006-1-SB.raw 0199_WUG_FARMM_FFA-9006-1-SB.raw
SUBJECT_SAMPLE_FACTORS           	9019	9019-1-SB	Study_Diet:Western | Sex:Male | Time:Day 12	Type=Stool; Age=36; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0214_WUG_FARMM_HIL-9019-1-SB.raw 0214_WUG_FARMM_LIP-9019-1-SB.raw 0214_WU_FARMM_CMH_9019-1-SB.raw 0214_WUG_FARMM_FFA-9019-1-SB.raw
SUBJECT_SAMPLE_FACTORS           	9030	9030-1-SB	Study_Diet:Western | Sex:Male | Time:Day 12	Type=Stool; Age=46; BMI=27.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0205_WUG_FARMM_HIL-9030-1-SB.raw 0205_WUG_FARMM_LIP-9030-1-SB.raw 0205_WU_FARMM_CMH_9030-1-SB.raw 0205_WUG_FARMM_FFA-9030-1-SB.raw
SUBJECT_SAMPLE_FACTORS           	9033	9033-1-SB	Study_Diet:Western | Sex:Male | Time:Day 12	Type=Stool; Age=57; BMI=27.1; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0182_WUG_FARMM_HIL-9033-1-SB.raw 0182_WUG_FARMM_LIP-9033-1-SB.raw 0182_WU_FARMM_CMH_9033-1-SB.raw 0182_WUG_FARMM_FFA-9033-1-SB.raw
SUBJECT_SAMPLE_FACTORS           	9036	9036-1-SB	Study_Diet:Western | Sex:Male | Time:Day 12	Type=Stool; Age=60; BMI=23.9; Ethnicity=Not Hispanic or Latino; Race=American Indian/Alaskan Native White; RAW_FILE_NAME=0234_WUG_FARMM_HIL-9036-1-SB.raw 0234_WUG_FARMM_LIP-9036-1-SB.raw 0234_WU_FARMM_CMH_9036-1-SB.raw 0234_WUG_FARMM_FFA-9036-1-SB.raw
SUBJECT_SAMPLE_FACTORS           	9037	9037-1-SB	Study_Diet:Western | Sex:Male | Time:Day 12	Type=Stool; Age=29; BMI=23; Ethnicity=Hispanic or Latino; Race=Black/African American White; RAW_FILE_NAME=0276_WUG_FARMM_HIL-9037-1-SB.raw 0276_WUG_FARMM_LIP-9037-1-SB.raw 0276_WU_FARMM_CMH_9037-1-SB.raw 0276_WUG_FARMM_FFA-9037-1-SB.raw
SUBJECT_SAMPLE_FACTORS           	9040	9040-1-SB	Study_Diet:Western | Sex:Male | Time:Day 12	Type=Stool; Age=22; BMI=23.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0267_WUG_FARMM_HIL-9040-1-SB.raw 0267_WUG_FARMM_LIP-9040-1-SB.raw 0267_WU_FARMM_CMH_9040-1-SB.raw 0267_WUG_FARMM_FFA-9040-1-SB.raw
SUBJECT_SAMPLE_FACTORS           	9010	9010-1-SA	Study_Diet:Western | Sex:Female | Time:Day 15	Type=Stool; Age=24; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0301_WUG_FARMM_HIL-9010-1-SA.raw 0301_WUG_FARMM_LIP-9010-1-SA.raw 0301_WU_FARMM_CMH_9010-1-SA.raw 0301_WUG_FARMM_FFA-9010-1-SA.raw
SUBJECT_SAMPLE_FACTORS           	9015	9015-1-SA	Study_Diet:Western | Sex:Female | Time:Day 15	Type=Stool; Age=23; BMI=20.5; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0248_WUG_FARMM_HIL-9015-1-SA.raw 0248_WUG_FARMM_LIP-9015-1-SA.raw 0248_WU_FARMM_CMH_9015-1-SA.raw 0248_WUG_FARMM_FFA-9015-1-SA.raw
SUBJECT_SAMPLE_FACTORS           	9005	9005-1-SA	Study_Diet:Western | Sex:Male | Time:Day 15	Type=Stool; Age=23; BMI=21.6; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0260_WUG_FARMM_HIL-9005-1-SA.raw 0260_WUG_FARMM_LIP-9005-1-SA.raw 0260_WU_FARMM_CMH_9005-1-SA.raw 0260_WUG_FARMM_FFA-9005-1-SA.raw
SUBJECT_SAMPLE_FACTORS           	9006	9006-1-SA	Study_Diet:Western | Sex:Male | Time:Day 15	Type=Stool; Age=24; BMI=28.2; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0198_WUG_FARMM_HIL-9006-1-SA.raw 0198_WUG_FARMM_LIP-9006-1-SA.raw 0198_WU_FARMM_CMH_9006-1-SA.raw 0198_WUG_FARMM_FFA-9006-1-SA.raw
SUBJECT_SAMPLE_FACTORS           	9019	9019-1-SA	Study_Diet:Western | Sex:Male | Time:Day 15	Type=Stool; Age=36; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0212_WUG_FARMM_HIL-9019-1-SA.raw 0212_WUG_FARMM_LIP-9019-1-SA.raw 0212_WU_FARMM_CMH_9019-1-SA.raw 0212_WUG_FARMM_FFA-9019-1-SA.raw
SUBJECT_SAMPLE_FACTORS           	9030	9030-1-SA	Study_Diet:Western | Sex:Male | Time:Day 15	Type=Stool; Age=46; BMI=27.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0204_WUG_FARMM_HIL-9030-1-SA.raw 0302f_0204re_WUG_FARMM_LIP-9030-1-SA.raw 0204_WU_FARMM_CMH_9030-1-SA.raw 0204_WUG_FARMM_FFA-9030-1-SA.raw
SUBJECT_SAMPLE_FACTORS           	9033	9033-1-SA	Study_Diet:Western | Sex:Male | Time:Day 15	Type=Stool; Age=57; BMI=27.1; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0184_WUG_FARMM_HIL-9033-1-SA.raw 0184_WUG_FARMM_LIP-9033-1-SA.raw 0184_WU_FARMM_CMH_9033-1-SA.raw 0184_WUG_FARMM_FFA-9033-1-SA.raw
SUBJECT_SAMPLE_FACTORS           	9036	9036-1-SA	Study_Diet:Western | Sex:Male | Time:Day 15	Type=Stool; Age=60; BMI=23.9; Ethnicity=Not Hispanic or Latino; Race=American Indian/Alaskan Native White; RAW_FILE_NAME=0232_WUG_FARMM_HIL-9036-1-SA.raw 0232_WUG_FARMM_LIP-9036-1-SA.raw 0232_WU_FARMM_CMH_9036-1-SA.raw 0232_WUG_FARMM_FFA-9036-1-SA.raw
SUBJECT_SAMPLE_FACTORS           	9037	9037-1-SA	Study_Diet:Western | Sex:Male | Time:Day 15	Type=Stool; Age=29; BMI=23; Ethnicity=Hispanic or Latino; Race=Black/African American White; RAW_FILE_NAME=0274_WUG_FARMM_HIL-9037-1-SA.raw 0274_WUG_FARMM_LIP-9037-1-SA.raw 0274_WU_FARMM_CMH_9037-1-SA.raw 0274_WUG_FARMM_FFA-9037-1-SA.raw
SUBJECT_SAMPLE_FACTORS           	9040	9040-1-SA	Study_Diet:Western | Sex:Male | Time:Day 15	Type=Stool; Age=22; BMI=23.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0271_WUG_FARMM_HIL-9040-1-SA.raw 0271_WUG_FARMM_LIP-9040-1-SA.raw 0271_WU_FARMM_CMH_9040-1-SA.raw 0271_WUG_FARMM_FFA-9040-1-SA.raw
SUBJECT_SAMPLE_FACTORS           	9014	9014-3-SE	Study_Diet:Vegan | Sex:Female | Time:Baseline	Type=Stool; Age=23; BMI=20.9; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0158_WUG_FARMM_HIL-9014-3-SE.raw 0158_WUG_FARMM_LIP-9014-3-SE.raw 0158_WU_FARMM_CMH_9014-3-SE.raw 0158_WUG_FARMM_FFA-9014-3-SE.raw
SUBJECT_SAMPLE_FACTORS           	9026	9026-3-SE	Study_Diet:Vegan | Sex:Female | Time:Baseline	Type=Stool; Age=24; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0188_WUG_FARMM_HIL-9026-3-SE.raw 0188_WUG_FARMM_LIP-9026-3-SE.raw 0188_WU_FARMM_CMH_9026-3-SE.raw 0188_WUG_FARMM_FFA-9026-3-SE.raw
SUBJECT_SAMPLE_FACTORS           	9027	9027-3-SE	Study_Diet:Vegan | Sex:Female | Time:Baseline	Type=Stool; Age=22; BMI=25.1; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0178_WUG_FARMM_HIL-9027-3-SE.raw 0178_WUG_FARMM_LIP-9027-3-SE.raw 0178_WU_FARMM_CMH_9027-3-SE.raw 0178_WUG_FARMM_FFA-9027-3-SE.raw
SUBJECT_SAMPLE_FACTORS           	9031	9031-3-SE	Study_Diet:Vegan | Sex:Female | Time:Baseline	Type=Stool; Age=32; BMI=22.8; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0168_WUG_FARMM_HIL-9031-3-SE.raw 0168_WUG_FARMM_LIP-9031-3-SE.raw 0168_WU_FARMM_CMH_9031-3-SE.raw 0168_WUG_FARMM_FFA-9031-3-SE.raw
SUBJECT_SAMPLE_FACTORS           	9002	9002-3-SE	Study_Diet:Vegan | Sex:Male | Time:Baseline	Type=Stool; Age=20; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0228_WUG_FARMM_HIL-9002-3-SE.raw 0228_WUG_FARMM_LIP-9002-3-SE.raw 0228_WU_FARMM_CMH_9002-3-SE.raw 0228_WUG_FARMM_FFA-9002-3-SE.raw
SUBJECT_SAMPLE_FACTORS           	9004	9004-3-SE	Study_Diet:Vegan | Sex:Male | Time:Baseline	Type=Stool; Age=24; BMI=21.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0245_WUG_FARMM_HIL-9004-3-SE.raw 0245_WUG_FARMM_LIP-9004-3-SE.raw 0245_WU_FARMM_CMH_9004-3-SE.raw 0245_WUG_FARMM_FFA-9004-3-SE.raw
SUBJECT_SAMPLE_FACTORS           	9008	9008-3-SE	Study_Diet:Vegan | Sex:Male | Time:Baseline	Type=Stool; Age=23; BMI=20; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0253_WUG_FARMM_HIL-9008-3-SE.raw 0253_WUG_FARMM_LIP-9008-3-SE.raw 0253_WU_FARMM_CMH_9008-3-SE.raw 0253_WUG_FARMM_FFA-9008-3-SE.raw
SUBJECT_SAMPLE_FACTORS           	9017	9017-3-SE	Study_Diet:Vegan | Sex:Male | Time:Baseline	Type=Stool; Age=25; BMI=20.6; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0210_WUG_FARMM_HIL-9017-3-SE.raw 0210_WUG_FARMM_LIP-9017-3-SE.raw 0210_WU_FARMM_CMH_9017-3-SE.raw 0210_WUG_FARMM_FFA-9017-3-SE.raw
SUBJECT_SAMPLE_FACTORS           	9022	9022-3-SE	Study_Diet:Vegan | Sex:Male | Time:Baseline	Type=Stool; Age=25; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0152_WUG_FARMM_HIL-9022-3-SE.raw 0152_WUG_FARMM_LIP-9022-3-SE.raw 0152_WU_FARMM_CMH_9022-3-SE.raw 0152_WUG_FARMM_FFA-9022-3-SE.raw
SUBJECT_SAMPLE_FACTORS           	9035	9035-3-SE	Study_Diet:Vegan | Sex:Male | Time:Baseline	Type=Stool; Age=25; BMI=19.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0291_WUG_FARMM_HIL-9035-3-SE.raw 0291_WUG_FARMM_LIP-9035-3-SE.raw 0291_WU_FARMM_CMH_9035-3-SE.raw 0291_WUG_FARMM_FFA-9035-3-SE.raw
SUBJECT_SAMPLE_FACTORS           	9014	9014-3-SD	Study_Diet:Vegan | Sex:Female | Time:Day 5	Type=Stool; Age=23; BMI=20.9; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0160_WUG_FARMM_HIL-9014-3-SD.raw 0160_WUG_FARMM_LIP-9014-3-SD.raw 0160_WU_FARMM_CMH_9014-3-SD.raw 0160_WUG_FARMM_FFA-9014-3-SD.raw
SUBJECT_SAMPLE_FACTORS           	9026	9026-3-SD	Study_Diet:Vegan | Sex:Female | Time:Day 5	Type=Stool; Age=24; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0187_WUG_FARMM_HIL-9026-3-SD.raw 0187_WUG_FARMM_LIP-9026-3-SD.raw 0187_WU_FARMM_CMH_9026-3-SD.raw 0187_WUG_FARMM_FFA-9026-3-SD.raw
SUBJECT_SAMPLE_FACTORS           	9027	9027-3-SD	Study_Diet:Vegan | Sex:Female | Time:Day 5	Type=Stool; Age=22; BMI=25.1; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0177_WUG_FARMM_HIL-9027-3-SD.raw 0177_WUG_FARMM_LIP-9027-3-SD.raw 0177_WU_FARMM_CMH_9027-3-SD.raw 0177_WUG_FARMM_FFA-9027-3-SD.raw
SUBJECT_SAMPLE_FACTORS           	9031	9031-3-SD	Study_Diet:Vegan | Sex:Female | Time:Day 5	Type=Stool; Age=32; BMI=22.8; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0167_WUG_FARMM_HIL-9031-3-SD.raw 0167_WUG_FARMM_LIP-9031-3-SD.raw 0167_WU_FARMM_CMH_9031-3-SD.raw 0167_WUG_FARMM_FFA-9031-3-SD.raw
SUBJECT_SAMPLE_FACTORS           	9002	9002-3-SD	Study_Diet:Vegan | Sex:Male | Time:Day 5	Type=Stool; Age=20; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0231_WUG_FARMM_HIL-9002-3-SD.raw 0231_WUG_FARMM_LIP-9002-3-SD.raw 0231_WU_FARMM_CMH_9002-3-SD.raw 0231_WUG_FARMM_FFA-9002-3-SD.raw
SUBJECT_SAMPLE_FACTORS           	9004	9004-3-SD	Study_Diet:Vegan | Sex:Male | Time:Day 5	Type=Stool; Age=24; BMI=21.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0244_WUG_FARMM_HIL-9004-3-SD.raw 0244_WUG_FARMM_LIP-9004-3-SD.raw 0244_WU_FARMM_CMH_9004-3-SD.raw 0244_WUG_FARMM_FFA-9004-3-SD.raw
SUBJECT_SAMPLE_FACTORS           	9008	9008-3-SD	Study_Diet:Vegan | Sex:Male | Time:Day 5	Type=Stool; Age=23; BMI=20; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0255_WUG_FARMM_HIL-9008-3-SD.raw 0255_WUG_FARMM_LIP-9008-3-SD.raw 0255_WU_FARMM_CMH_9008-3-SD.raw 0255_WUG_FARMM_FFA-9008-3-SD.raw
SUBJECT_SAMPLE_FACTORS           	9017	9017-3-SD	Study_Diet:Vegan | Sex:Male | Time:Day 5	Type=Stool; Age=25; BMI=20.6; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0211_WUG_FARMM_HIL-9017-3-SD.raw 0211_WUG_FARMM_LIP-9017-3-SD.raw 0211_WU_FARMM_CMH_9017-3-SD.raw 0211_WUG_FARMM_FFA-9017-3-SD.raw
SUBJECT_SAMPLE_FACTORS           	9022	9022-3-SD	Study_Diet:Vegan | Sex:Male | Time:Day 5	Type=Stool; Age=25; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0153_WUG_FARMM_HIL-9022-3-SD.raw 0153_WUG_FARMM_LIP-9022-3-SD.raw 0153_WU_FARMM_CMH_9022-3-SD.raw 0153_WUG_FARMM_FFA-9022-3-SD.raw
SUBJECT_SAMPLE_FACTORS           	9035	9035-3-SD	Study_Diet:Vegan | Sex:Male | Time:Day 5	Type=Stool; Age=25; BMI=19.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0287_WUG_FARMM_HIL-9035-3-SD.raw 0287_WUG_FARMM_LIP-9035-3-SD.raw 0287_WU_FARMM_CMH_9035-3-SD.raw 0287_WUG_FARMM_FFA-9035-3-SD.raw
SUBJECT_SAMPLE_FACTORS           	9014	9014-3-SC	Study_Diet:Vegan | Sex:Female | Time:Day 9	Type=Stool; Age=23; BMI=20.9; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0157_WUG_FARMM_HIL-9014-3-SC.raw 0157_WUG_FARMM_LIP-9014-3-SC.raw 0157_WU_FARMM_CMH_9014-3-SC.raw 0157_WUG_FARMM_FFA-9014-3-SC.raw
SUBJECT_SAMPLE_FACTORS           	9026	9026-3-SC	Study_Diet:Vegan | Sex:Female | Time:Day 9	Type=Stool; Age=24; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0189_WUG_FARMM_HIL-9026-3-SC.raw 0189_WUG_FARMM_LIP-9026-3-SC.raw 0189_WU_FARMM_CMH_9026-3-SC.raw 0189_WUG_FARMM_FFA-9026-3-SC.raw
SUBJECT_SAMPLE_FACTORS           	9027	9027-3-SC	Study_Diet:Vegan | Sex:Female | Time:Day 9	Type=Stool; Age=22; BMI=25.1; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0180_WUG_FARMM_HIL-9027-3-SC.raw 0180_WUG_FARMM_LIP-9027-3-SC.raw 0180_WU_FARMM_CMH_9027-3-SC.raw 0180_WUG_FARMM_FFA-9027-3-SC.raw
SUBJECT_SAMPLE_FACTORS           	9031	9031-3-SC	Study_Diet:Vegan | Sex:Female | Time:Day 9	Type=Stool; Age=32; BMI=22.8; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0171_WUG_FARMM_HIL-9031-3-SC.raw 0171_WUG_FARMM_LIP-9031-3-SC.raw 0171_WU_FARMM_CMH_9031-3-SC.raw 0171_WUG_FARMM_FFA-9031-3-SC.raw
SUBJECT_SAMPLE_FACTORS           	9002	9002-3-SC	Study_Diet:Vegan | Sex:Male | Time:Day 9	Type=Stool; Age=20; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0230_WUG_FARMM_HIL-9002-3-SC.raw 0230_WUG_FARMM_LIP-9002-3-SC.raw 0230_WU_FARMM_CMH_9002-3-SC.raw 0230_WUG_FARMM_FFA-9002-3-SC.raw
SUBJECT_SAMPLE_FACTORS           	9004	9004-3-SC	Study_Diet:Vegan | Sex:Male | Time:Day 9	Type=Stool; Age=24; BMI=21.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0246_WUG_FARMM_HIL-9004-3-SC.raw 0246_WUG_FARMM_LIP-9004-3-SC.raw 0246_WU_FARMM_CMH_9004-3-SC.raw 0246_WUG_FARMM_FFA-9004-3-SC.raw
SUBJECT_SAMPLE_FACTORS           	9008	9008-3-SC	Study_Diet:Vegan | Sex:Male | Time:Day 9	Type=Stool; Age=23; BMI=20; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0252_WUG_FARMM_HIL-9008-3-SC.raw 0252_WUG_FARMM_LIP-9008-3-SC.raw 0252_WU_FARMM_CMH_9008-3-SC.raw 0252_WUG_FARMM_FFA-9008-3-SC.raw
SUBJECT_SAMPLE_FACTORS           	9017	9017-3-SC	Study_Diet:Vegan | Sex:Male | Time:Day 9	Type=Stool; Age=25; BMI=20.6; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0209_WUG_FARMM_HIL-9017-3-SC.raw 0209_WUG_FARMM_LIP-9017-3-SC.raw 0209_WU_FARMM_CMH_9017-3-SC.raw 0209_WUG_FARMM_FFA-9017-3-SC.raw
SUBJECT_SAMPLE_FACTORS           	9022	9022-3-SC	Study_Diet:Vegan | Sex:Male | Time:Day 9	Type=Stool; Age=25; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0155_WUG_FARMM_HIL-9022-3-SC.raw 0155_WUG_FARMM_LIP-9022-3-SC.raw 0155_WU_FARMM_CMH_9022-3-SC.raw 0155_WUG_FARMM_FFA-9022-3-SC.raw
SUBJECT_SAMPLE_FACTORS           	9035	9035-3-SC	Study_Diet:Vegan | Sex:Male | Time:Day 9	Type=Stool; Age=25; BMI=19.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0288_WUG_FARMM_HIL-9035-3-SC.raw 0288_WUG_FARMM_LIP-9035-3-SC.raw 0288_WU_FARMM_CMH_9035-3-SC.raw 0288_WUG_FARMM_FFA-9035-3-SC.raw
SUBJECT_SAMPLE_FACTORS           	9014	9014-3-SB	Study_Diet:Vegan | Sex:Female | Time:Day 12	Type=Stool; Age=23; BMI=20.9; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0159_WUG_FARMM_HIL-9014-3-SB.raw 0159_WUG_FARMM_LIP-9014-3-SB.raw 0159_WU_FARMM_CMH_9014-3-SB.raw 0159_WUG_FARMM_FFA-9014-3-SB.raw
SUBJECT_SAMPLE_FACTORS           	9026	9026-3-SB	Study_Diet:Vegan | Sex:Female | Time:Day 12	Type=Stool; Age=24; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0191_WUG_FARMM_HIL-9026-3-SB.raw 0191_WUG_FARMM_LIP-9026-3-SB.raw 0191_WU_FARMM_CMH_9026-3-SB.raw 0191_WUG_FARMM_FFA-9026-3-SB.raw
SUBJECT_SAMPLE_FACTORS           	9027	9027-3-SB	Study_Diet:Vegan | Sex:Female | Time:Day 12	Type=Stool; Age=22; BMI=25.1; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0179_WUG_FARMM_HIL-9027-3-SB.raw 0179_WUG_FARMM_LIP-9027-3-SB.raw 0179_WU_FARMM_CMH_9027-3-SB.raw 0179_WUG_FARMM_FFA-9027-3-SB.raw
SUBJECT_SAMPLE_FACTORS           	9031	9031-3-SB	Study_Diet:Vegan | Sex:Female | Time:Day 12	Type=Stool; Age=32; BMI=22.8; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0169_WUG_FARMM_HIL-9031-3-SB.raw 0169_WUG_FARMM_LIP-9031-3-SB.raw 0169_WU_FARMM_CMH_9031-3-SB.raw 0169_WUG_FARMM_FFA-9031-3-SB.raw
SUBJECT_SAMPLE_FACTORS           	9002	9002-3-SB	Study_Diet:Vegan | Sex:Male | Time:Day 12	Type=Stool; Age=20; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0227_WUG_FARMM_HIL-9002-3-SB.raw 0227_WUG_FARMM_LIP-9002-3-SB.raw 0227_WU_FARMM_CMH_9002-3-SB.raw 0227_WUG_FARMM_FFA-9002-3-SB.raw
SUBJECT_SAMPLE_FACTORS           	9004	9004-3-SB	Study_Diet:Vegan | Sex:Male | Time:Day 12	Type=Stool; Age=24; BMI=21.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0243_WUG_FARMM_HIL-9004-3-SB.raw 0243_WUG_FARMM_LIP-9004-3-SB.raw 0243_WU_FARMM_CMH_9004-3-SB.raw 0243_WUG_FARMM_FFA-9004-3-SB.raw
SUBJECT_SAMPLE_FACTORS           	9008	9008-3-SB	Study_Diet:Vegan | Sex:Male | Time:Day 12	Type=Stool; Age=23; BMI=20; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0256_WUG_FARMM_HIL-9008-3-SB.raw 0256_WUG_FARMM_LIP-9008-3-SB.raw 0256_WU_FARMM_CMH_9008-3-SB.raw 0256_WUG_FARMM_FFA-9008-3-SB.raw
SUBJECT_SAMPLE_FACTORS           	9017	9017-3-SB	Study_Diet:Vegan | Sex:Male | Time:Day 12	Type=Stool; Age=25; BMI=20.6; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0207_WUG_FARMM_HIL-9017-3-SB.raw 0207_WUG_FARMM_LIP-9017-3-SB.raw 0207_WU_FARMM_CMH_9017-3-SB.raw 0207_WUG_FARMM_FFA-9017-3-SB.raw
SUBJECT_SAMPLE_FACTORS           	9022	9022-3-SB	Study_Diet:Vegan | Sex:Male | Time:Day 12	Type=Stool; Age=25; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0154_WUG_FARMM_HIL-9022-3-SB.raw 0154_WUG_FARMM_LIP-9022-3-SB.raw 0154_WU_FARMM_CMH_9022-3-SB.raw 0154_WUG_FARMM_FFA-9022-3-SB.raw
SUBJECT_SAMPLE_FACTORS           	9035	9035-3-SB	Study_Diet:Vegan | Sex:Male | Time:Day 12	Type=Stool; Age=25; BMI=19.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0289_WUG_FARMM_HIL-9035-3-SB.raw 0289_WUG_FARMM_LIP-9035-3-SB.raw 0289_WU_FARMM_CMH_9035-3-SB.raw 0289_WUG_FARMM_FFA-9035-3-SB.raw
SUBJECT_SAMPLE_FACTORS           	9014	9014-3-SA	Study_Diet:Vegan | Sex:Female | Time:Day 15	Type=Stool; Age=23; BMI=20.9; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0161_WUG_FARMM_HIL-9014-3-SA.raw 0161_WUG_FARMM_LIP-9014-3-SA.raw 0161_WU_FARMM_CMH_9014-3-SA.raw 0161_WUG_FARMM_FFA-9014-3-SA.raw
SUBJECT_SAMPLE_FACTORS           	9026	9026-3-SA	Study_Diet:Vegan | Sex:Female | Time:Day 15	Type=Stool; Age=24; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0190_WUG_FARMM_HIL-9026-3-SA.raw 0190_WUG_FARMM_LIP-9026-3-SA.raw 0190_WU_FARMM_CMH_9026-3-SA.raw 0190_WUG_FARMM_FFA-9026-3-SA.raw
SUBJECT_SAMPLE_FACTORS           	9031	9031-3-SA	Study_Diet:Vegan | Sex:Female | Time:Day 15	Type=Stool; Age=32; BMI=22.8; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0170_WUG_FARMM_HIL-9031-3-SA.raw 0221b_0170ri_WUG_FARMM_LIP-9031-3-SA.raw 0170_WU_FARMM_CMH_9031-3-SA.raw 0170_WUG_FARMM_FFA-9031-3-SA.raw
SUBJECT_SAMPLE_FACTORS           	9002	9002-3-SA	Study_Diet:Vegan | Sex:Male | Time:Day 15	Type=Stool; Age=20; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0229_WUG_FARMM_HIL-9002-3-SA.raw 0229_WUG_FARMM_LIP-9002-3-SA.raw 0229_WU_FARMM_CMH_9002-3-SA.raw 0229_WUG_FARMM_FFA-9002-3-SA.raw
SUBJECT_SAMPLE_FACTORS           	9004	9004-3-SA	Study_Diet:Vegan | Sex:Male | Time:Day 15	Type=Stool; Age=24; BMI=21.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0242_WUG_FARMM_HIL-9004-3-SA.raw 0242_WUG_FARMM_LIP-9004-3-SA.raw 0242_WU_FARMM_CMH_9004-3-SA.raw 0242_WUG_FARMM_FFA-9004-3-SA.raw
SUBJECT_SAMPLE_FACTORS           	9008	9008-3-SA	Study_Diet:Vegan | Sex:Male | Time:Day 15	Type=Stool; Age=23; BMI=20; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0254_WUG_FARMM_HIL-9008-3-SA.raw 0254_WUG_FARMM_LIP-9008-3-SA.raw 0254_WU_FARMM_CMH_9008-3-SA.raw 0254_WUG_FARMM_FFA-9008-3-SA.raw
SUBJECT_SAMPLE_FACTORS           	9017	9017-3-SA	Study_Diet:Vegan | Sex:Male | Time:Day 15	Type=Stool; Age=25; BMI=20.6; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0208_WUG_FARMM_HIL-9017-3-SA.raw 0208_WUG_FARMM_LIP-9017-3-SA.raw 0208_WU_FARMM_CMH_9017-3-SA.raw 0208_WUG_FARMM_FFA-9017-3-SA.raw
SUBJECT_SAMPLE_FACTORS           	9022	9022-3-SA	Study_Diet:Vegan | Sex:Male | Time:Day 15	Type=Stool; Age=25; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0156_WUG_FARMM_HIL-9022-3-SA.raw 0156_WUG_FARMM_LIP-9022-3-SA.raw 0156_WU_FARMM_CMH_9022-3-SA.raw 0156_WUG_FARMM_FFA-9022-3-SA.raw
SUBJECT_SAMPLE_FACTORS           	9035	9035-3-SA	Study_Diet:Vegan | Sex:Male | Time:Day 15	Type=Stool; Age=25; BMI=19.7; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0290_WUG_FARMM_HIL-9035-3-SA.raw 0290_WUG_FARMM_LIP-9035-3-SA.raw 0290_WU_FARMM_CMH_9035-3-SA.raw 0290_WUG_FARMM_FFA-9035-3-SA.raw
SUBJECT_SAMPLE_FACTORS           	9016	9016-2-SE	Study_Diet:Modulen | Sex:Female | Time:Baseline	Type=Stool; Age=38; BMI=25.6; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0220_WUG_FARMM_HIL-9016-2-SE.raw 0220_WUG_FARMM_LIP-9016-2-SE.raw 0220_WU_FARMM_CMH_9016-2-SE.raw 0220_WUG_FARMM_FFA-9016-2-SE.raw
SUBJECT_SAMPLE_FACTORS           	9024	9024-2-SE	Study_Diet:Modulen | Sex:Female | Time:Baseline	Type=Stool; Age=27; BMI=34.6; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0162_WUG_FARMM_HIL-9024-2-SE.raw 0162_WUG_FARMM_LIP-9024-2-SE.raw 0162_WU_FARMM_CMH_9024-2-SE.raw 0162_WUG_FARMM_FFA-9024-2-SE.raw
SUBJECT_SAMPLE_FACTORS           	9034	9034-2-SE	Study_Diet:Modulen | Sex:Female | Time:Baseline	Type=Stool; Age=43; BMI=25.3; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0173_WUG_FARMM_HIL-9034-2-SE.raw 0173_WUG_FARMM_LIP-9034-2-SE.raw 0173_WU_FARMM_CMH_9034-2-SE.raw 0173_WUG_FARMM_FFA-9034-2-SE.raw
SUBJECT_SAMPLE_FACTORS           	9038	9038-2-SE	Study_Diet:Modulen | Sex:Female | Time:Baseline	Type=Stool; Age=59; BMI=34.3; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0238_WUG_FARMM_HIL-9038-2-SE.raw 0238_WUG_FARMM_LIP-9038-2-SE.raw 0238_WU_FARMM_CMH_9038-2-SE.raw 0238_WUG_FARMM_FFA-9038-2-SE.raw
SUBJECT_SAMPLE_FACTORS           	9003	9003-2-SE	Study_Diet:Modulen | Sex:Male | Time:Baseline	Type=Stool; Age=20; BMI=20.3; Ethnicity=NA; Race=White; RAW_FILE_NAME=0297_WUG_FARMM_HIL-9003-2-SE.raw 0297_WUG_FARMM_LIP-9003-2-SE.raw 0297_WU_FARMM_CMH_9003-2-SE.raw 0297_WUG_FARMM_FFA-9003-2-SE.raw
SUBJECT_SAMPLE_FACTORS           	9009	9009-2-SE	Study_Diet:Modulen | Sex:Male | Time:Baseline	Type=Stool; Age=23; BMI=22.4; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0286_WUG_FARMM_HIL-9009-2-SE.raw 0286_WUG_FARMM_LIP-9009-2-SE.raw 0286_WU_FARMM_CMH_9009-2-SE.raw 0286_WUG_FARMM_FFA-9009-2-SE.raw
SUBJECT_SAMPLE_FACTORS           	9013	9013-2-SE	Study_Diet:Modulen | Sex:Male | Time:Baseline	Type=Stool; Age=40; BMI=24.1; Ethnicity=Hispanic or Latino; Race=White; RAW_FILE_NAME=0224_WUG_FARMM_HIL-9013-2-SE.raw 0224_WUG_FARMM_LIP-9013-2-SE.raw 0224_WU_FARMM_CMH_9013-2-SE.raw 0224_WUG_FARMM_FFA-9013-2-SE.raw
SUBJECT_SAMPLE_FACTORS           	9025	9025-2-SE	Study_Diet:Modulen | Sex:Male | Time:Baseline	Type=Stool; Age=28; BMI=30.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0193_WUG_FARMM_HIL-9025-2-SE.raw 0193_WUG_FARMM_LIP-9025-2-SE.raw 0193_WU_FARMM_CMH_9025-2-SE.raw 0193_WUG_FARMM_FFA-9025-2-SE.raw
SUBJECT_SAMPLE_FACTORS           	9029	9029-2-SE	Study_Diet:Modulen | Sex:Male | Time:Baseline	Type=Stool; Age=26; BMI=35.4; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0280_WUG_FARMM_HIL-9029-2-SE.raw 0280_WUG_FARMM_LIP-9029-2-SE.raw 0280_WU_FARMM_CMH_9029-2-SE.raw 0280_WUG_FARMM_FFA-9029-2-SE.raw
SUBJECT_SAMPLE_FACTORS           	9032	9032-2-SE	Study_Diet:Modulen | Sex:Male | Time:Baseline	Type=Stool; Age=53; BMI=31; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0266_WUG_FARMM_HIL-9032-2-SE.raw 0266_WUG_FARMM_LIP-9032-2-SE.raw 0266_WU_FARMM_CMH_9032-2-SE.raw 0266_WUG_FARMM_FFA-9032-2-SE.raw
SUBJECT_SAMPLE_FACTORS           	9016	9016-2-SD	Study_Diet:Modulen | Sex:Female | Time:Day 5	Type=Stool; Age=38; BMI=25.6; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0219_WUG_FARMM_HIL-9016-2-SD.raw 0219_WUG_FARMM_LIP-9016-2-SD.raw 0219_WU_FARMM_CMH_9016-2-SD.raw 0219_WUG_FARMM_FFA-9016-2-SD.raw
SUBJECT_SAMPLE_FACTORS           	9024	9024-2-SD	Study_Diet:Modulen | Sex:Female | Time:Day 5	Type=Stool; Age=27; BMI=34.6; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0165_WUG_FARMM_HIL-9024-2-SD.raw 0165_WUG_FARMM_LIP-9024-2-SD.raw 0165_WU_FARMM_CMH_9024-2-SD.raw 0165_WUG_FARMM_FFA-9024-2-SD.raw
SUBJECT_SAMPLE_FACTORS           	9034	9034-2-SD	Study_Diet:Modulen | Sex:Female | Time:Day 5	Type=Stool; Age=43; BMI=25.3; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0172_WUG_FARMM_HIL-9034-2-SD.raw 0172_WUG_FARMM_LIP-9034-2-SD.raw 0172_WU_FARMM_CMH_9034-2-SD.raw 0172_WUG_FARMM_FFA-9034-2-SD.raw
SUBJECT_SAMPLE_FACTORS           	9038	9038-2-SD	Study_Diet:Modulen | Sex:Female | Time:Day 5	Type=Stool; Age=59; BMI=34.3; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0240_WUG_FARMM_HIL-9038-2-SD.raw 0240_WUG_FARMM_LIP-9038-2-SD.raw 0240_WU_FARMM_CMH_9038-2-SD.raw 0240_WUG_FARMM_FFA-9038-2-SD.raw
SUBJECT_SAMPLE_FACTORS           	9009	9009-2-SD	Study_Diet:Modulen | Sex:Male | Time:Day 5	Type=Stool; Age=23; BMI=22.4; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0285_WUG_FARMM_HIL-9009-2-SD.raw 0302f_0285ri_WUG_FARMM_LIP-9009-2-SD.raw 0285_WU_FARMM_CMH_9009-2-SD.raw 0285_WUG_FARMM_FFA-9009-2-SD.raw
SUBJECT_SAMPLE_FACTORS           	9013	9013-2-SD	Study_Diet:Modulen | Sex:Male | Time:Day 5	Type=Stool; Age=40; BMI=24.1; Ethnicity=Hispanic or Latino; Race=White; RAW_FILE_NAME=0222_WUG_FARMM_HIL-9013-2-SD.raw 0222_WUG_FARMM_LIP-9013-2-SD.raw 0222_WU_FARMM_CMH_9013-2-SD.raw 0222_WUG_FARMM_FFA-9013-2-SD.raw
SUBJECT_SAMPLE_FACTORS           	9025	9025-2-SD	Study_Diet:Modulen | Sex:Male | Time:Day 5	Type=Stool; Age=28; BMI=30.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0195_WUG_FARMM_HIL-9025-2-SD.raw 0195_WUG_FARMM_LIP-9025-2-SD.raw 0195_WU_FARMM_CMH_9025-2-SD.raw 0195_WUG_FARMM_FFA-9025-2-SD.raw
SUBJECT_SAMPLE_FACTORS           	9029	9029-2-SD	Study_Diet:Modulen | Sex:Male | Time:Day 5	Type=Stool; Age=26; BMI=35.4; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0278_WUG_FARMM_HIL-9029-2-SD.raw 0278_WUG_FARMM_LIP-9029-2-SD.raw 0278_WU_FARMM_CMH_9029-2-SD.raw 0278_WUG_FARMM_FFA-9029-2-SD.raw
SUBJECT_SAMPLE_FACTORS           	9032	9032-2-SD	Study_Diet:Modulen | Sex:Male | Time:Day 5	Type=Stool; Age=53; BMI=31; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0264_WUG_FARMM_HIL-9032-2-SD.raw 0264_WUG_FARMM_LIP-9032-2-SD.raw 0264_WU_FARMM_CMH_9032-2-SD.raw 0264_WUG_FARMM_FFA-9032-2-SD.raw
SUBJECT_SAMPLE_FACTORS           	9016	9016-2-SC	Study_Diet:Modulen | Sex:Female | Time:Day 9	Type=Stool; Age=38; BMI=25.6; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0221_WUG_FARMM_HIL-9016-2-SC.raw 0221_WUG_FARMM_LIP-9016-2-SC.raw 0221_WU_FARMM_CMH_9016-2-SC.raw 0221_WUG_FARMM_FFA-9016-2-SC.raw
SUBJECT_SAMPLE_FACTORS           	9034	9034-2-SC	Study_Diet:Modulen | Sex:Female | Time:Day 9	Type=Stool; Age=43; BMI=25.3; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0176_WUG_FARMM_HIL-9034-2-SC.raw 0176_WUG_FARMM_LIP-9034-2-SC.raw 0176_WU_FARMM_CMH_9034-2-SC.raw 0176_WUG_FARMM_FFA-9034-2-SC.raw
SUBJECT_SAMPLE_FACTORS           	9038	9038-2-SC	Study_Diet:Modulen | Sex:Female | Time:Day 9	Type=Stool; Age=59; BMI=34.3; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0239_WUG_FARMM_HIL-9038-2-SC.raw 0239_WUG_FARMM_LIP-9038-2-SC.raw 0239_WU_FARMM_CMH_9038-2-SC.raw 0239_WUG_FARMM_FFA-9038-2-SC.raw
SUBJECT_SAMPLE_FACTORS           	9003	9003-2-SC	Study_Diet:Modulen | Sex:Male | Time:Day 9	Type=Stool; Age=20; BMI=20.3; Ethnicity=NA; Race=White; RAW_FILE_NAME=0295_WUG_FARMM_HIL-9003-2-SC.raw 0295_WUG_FARMM_LIP-9003-2-SC.raw 0295_WU_FARMM_CMH_9003-2-SC.raw 0295_WUG_FARMM_FFA-9003-2-SC.raw
SUBJECT_SAMPLE_FACTORS           	9009	9009-2-SC	Study_Diet:Modulen | Sex:Male | Time:Day 9	Type=Stool; Age=23; BMI=22.4; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0284_WUG_FARMM_HIL-9009-2-SC.raw 0284_WUG_FARMM_LIP-9009-2-SC.raw 0284_WU_FARMM_CMH_9009-2-SC.raw 0284_WUG_FARMM_FFA-9009-2-SC.raw
SUBJECT_SAMPLE_FACTORS           	9013	9013-2-SC	Study_Diet:Modulen | Sex:Male | Time:Day 9	Type=Stool; Age=40; BMI=24.1; Ethnicity=Hispanic or Latino; Race=White; RAW_FILE_NAME=0223_WUG_FARMM_HIL-9013-2-SC.raw 0223_WUG_FARMM_LIP-9013-2-SC.raw 0223_WU_FARMM_CMH_9013-2-SC.raw 0223_WUG_FARMM_FFA-9013-2-SC.raw
SUBJECT_SAMPLE_FACTORS           	9025	9025-2-SC	Study_Diet:Modulen | Sex:Male | Time:Day 9	Type=Stool; Age=28; BMI=30.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0194_WUG_FARMM_HIL-9025-2-SC.raw 0194_WUG_FARMM_LIP-9025-2-SC.raw 0194_WU_FARMM_CMH_9025-2-SC.raw 0194_WUG_FARMM_FFA-9025-2-SC.raw
SUBJECT_SAMPLE_FACTORS           	9029	9029-2-SC	Study_Diet:Modulen | Sex:Male | Time:Day 9	Type=Stool; Age=26; BMI=35.4; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0281_WUG_FARMM_HIL-9029-2-SC.raw 0281_WUG_FARMM_LIP-9029-2-SC.raw 0281_WU_FARMM_CMH_9029-2-SC.raw 0281_WUG_FARMM_FFA-9029-2-SC.raw
SUBJECT_SAMPLE_FACTORS           	9032	9032-2-SC	Study_Diet:Modulen | Sex:Male | Time:Day 9	Type=Stool; Age=53; BMI=31; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0263_WUG_FARMM_HIL-9032-2-SC_160922155707.raw 0302c_0263ri_WUG_FARMM_LIP-9032-2-SC.raw 0263_WU_FARMM_CMH_9032-2-SC.raw 0263_WUG_FARMM_FFA-9032-2-SC.raw
SUBJECT_SAMPLE_FACTORS           	9016	9016-2-SB	Study_Diet:Modulen | Sex:Female | Time:Day 12	Type=Stool; Age=38; BMI=25.6; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0217_WUG_FARMM_HIL-9016-2-SB.raw 0217_WUG_FARMM_LIP-9016-2-SB.raw 0217_WU_FARMM_CMH_9016-2-SB.raw 0217_WUG_FARMM_FFA-9016-2-SB.raw
SUBJECT_SAMPLE_FACTORS           	9024	9024-2-SB	Study_Diet:Modulen | Sex:Female | Time:Day 12	Type=Stool; Age=27; BMI=34.6; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0164_WUG_FARMM_HIL-9024-2-SB.raw 0164_WUG_FARMM_LIP-9024-2-SB.raw 0164_WU_FARMM_CMH_9024-2-SB.raw 0164_WUG_FARMM_FFA-9024-2-SB.raw
SUBJECT_SAMPLE_FACTORS           	9034	9034-2-SB	Study_Diet:Modulen | Sex:Female | Time:Day 12	Type=Stool; Age=43; BMI=25.3; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0174_WUG_FARMM_HIL-9034-2-SB.raw 0174_WUG_FARMM_LIP-9034-2-SB.raw 0174_WU_FARMM_CMH_9034-2-SB.raw 0174_WUG_FARMM_FFA-9034-2-SB.raw
SUBJECT_SAMPLE_FACTORS           	9038	9038-2-SB	Study_Diet:Modulen | Sex:Female | Time:Day 12	Type=Stool; Age=59; BMI=34.3; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0241_WUG_FARMM_HIL-9038-2-SB.raw 0241_WUG_FARMM_LIP-9038-2-SB.raw 0241_WU_FARMM_CMH_9038-2-SB.raw 0241_WUG_FARMM_FFA-9038-2-SB.raw
SUBJECT_SAMPLE_FACTORS           	9003	9003-2-SB	Study_Diet:Modulen | Sex:Male | Time:Day 12	Type=Stool; Age=20; BMI=20.3; Ethnicity=NA; Race=White; RAW_FILE_NAME=0293_WUG_FARMM_HIL-9003-2-SB.raw 0293_WUG_FARMM_LIP-9003-2-SB.raw 0293_WU_FARMM_CMH_9003-2-SB.raw 0293_WUG_FARMM_FFA-9003-2-SB.raw
SUBJECT_SAMPLE_FACTORS           	9009	9009-2-SB	Study_Diet:Modulen | Sex:Male | Time:Day 12	Type=Stool; Age=23; BMI=22.4; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0283_WUG_FARMM_HIL-9009-2-SB.raw 0283_WUG_FARMM_LIP-9009-2-SB.raw 0283_WU_FARMM_CMH_9009-2-SB.raw 0283_WUG_FARMM_FFA-9009-2-SB.raw
SUBJECT_SAMPLE_FACTORS           	9013	9013-2-SB	Study_Diet:Modulen | Sex:Male | Time:Day 12	Type=Stool; Age=40; BMI=24.1; Ethnicity=Hispanic or Latino; Race=White; RAW_FILE_NAME=0225_WUG_FARMM_HIL-9013-2-SB.raw 0225_WUG_FARMM_LIP-9013-2-SB.raw 0225_WU_FARMM_CMH_9013-2-SB.raw 0225_WUG_FARMM_FFA-9013-2-SB.raw
SUBJECT_SAMPLE_FACTORS           	9025	9025-2-SB	Study_Diet:Modulen | Sex:Male | Time:Day 12	Type=Stool; Age=28; BMI=30.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0196_WUG_FARMM_HIL-9025-2-SB.raw 0302e_0196re_WUG_FARMM_LIP-9025-2-SB.raw 0196_WU_FARMM_CMH_9025-2-SB.raw 0196_WUG_FARMM_FFA-9025-2-SB.raw
SUBJECT_SAMPLE_FACTORS           	9029	9029-2-SB	Study_Diet:Modulen | Sex:Male | Time:Day 12	Type=Stool; Age=26; BMI=35.4; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0277_WUG_FARMM_HIL-9029-2-SB.raw 0277_WUG_FARMM_LIP-9029-2-SB.raw 0277_WU_FARMM_CMH_9029-2-SB.raw 0277_WUG_FARMM_FFA-9029-2-SB.raw
SUBJECT_SAMPLE_FACTORS           	9032	9032-2-SB	Study_Diet:Modulen | Sex:Male | Time:Day 12	Type=Stool; Age=53; BMI=31; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0265_WUG_FARMM_HIL-9032-2-SB.raw 0302d_0265ri_WUG_FARMM_LIP-9032-2-SB.raw 0265_WU_FARMM_CMH_9032-2-SB.raw 0265_WUG_FARMM_FFA-9032-2-SB.raw
SUBJECT_SAMPLE_FACTORS           	9016	9016-2-SA	Study_Diet:Modulen | Sex:Female | Time:Day 15	Type=Stool; Age=38; BMI=25.6; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0218_WUG_FARMM_HIL-9016-2-SA.raw 0218_WUG_FARMM_LIP-9016-2-SA.raw 0218_WU_FARMM_CMH_9016-2-SA.raw 0218_WUG_FARMM_FFA-9016-2-SA.raw
SUBJECT_SAMPLE_FACTORS           	9024	9024-2-SA	Study_Diet:Modulen | Sex:Female | Time:Day 15	Type=Stool; Age=27; BMI=34.6; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0163_WUG_FARMM_HIL-9024-2-SA.raw 0163_WUG_FARMM_LIP-9024-2-SA.raw 0163_WU_FARMM_CMH_9024-2-SA.raw 0163_WUG_FARMM_FFA-9024-2-SA.raw
SUBJECT_SAMPLE_FACTORS           	9034	9034-2-SA	Study_Diet:Modulen | Sex:Female | Time:Day 15	Type=Stool; Age=43; BMI=25.3; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0175_WUG_FARMM_HIL-9034-2-SA.raw 0175_WUG_FARMM_LIP-9034-2-SA.raw 0175_WU_FARMM_CMH_9034-2-SA.raw 0175_WUG_FARMM_FFA-9034-2-SA.raw
SUBJECT_SAMPLE_FACTORS           	9038	9038-2-SA	Study_Diet:Modulen | Sex:Female | Time:Day 15	Type=Stool; Age=59; BMI=34.3; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0237_WUG_FARMM_HIL-9038-2-SA.raw 0237_WUG_FARMM_LIP-9038-2-SA.raw 0237_WU_FARMM_CMH_9038-2-SA.raw 0237_WUG_FARMM_FFA-9038-2-SA.raw
SUBJECT_SAMPLE_FACTORS           	9003	9003-2-SA	Study_Diet:Modulen | Sex:Male | Time:Day 15	Type=Stool; Age=20; BMI=20.3; Ethnicity=NA; Race=White; RAW_FILE_NAME=0296_WUG_FARMM_HIL-9003-2-SA.raw 0296_WUG_FARMM_LIP-9003-2-SA.raw 0296_WU_FARMM_CMH_9003-2-SA.raw 0296_WUG_FARMM_FFA-9003-2-SA.raw
SUBJECT_SAMPLE_FACTORS           	9009	9009-2-SA	Study_Diet:Modulen | Sex:Male | Time:Day 15	Type=Stool; Age=23; BMI=22.4; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0282_WUG_FARMM_HIL-9009-2-SA.raw 0282_WUG_FARMM_LIP-9009-2-SA.raw 0282_WU_FARMM_CMH_9009-2-SA.raw 0282_WUG_FARMM_FFA-9009-2-SA.raw
SUBJECT_SAMPLE_FACTORS           	9013	9013-2-SA	Study_Diet:Modulen | Sex:Male | Time:Day 15	Type=Stool; Age=40; BMI=24.1; Ethnicity=Hispanic or Latino; Race=White; RAW_FILE_NAME=0226_WUG_FARMM_HIL-9013-2-SA.raw 0226_WUG_FARMM_LIP-9013-2-SA.raw 0226_WU_FARMM_CMH_9013-2-SA.raw 0226_WUG_FARMM_FFA-9013-2-SA.raw
SUBJECT_SAMPLE_FACTORS           	9025	9025-2-SA	Study_Diet:Modulen | Sex:Male | Time:Day 15	Type=Stool; Age=28; BMI=30.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; RAW_FILE_NAME=0192_WUG_FARMM_HIL-9025-2-SA.raw 0192_WUG_FARMM_LIP-9025-2-SA.raw 0192_WU_FARMM_CMH_9025-2-SA.raw 0192_WUG_FARMM_FFA-9025-2-SA.raw
SUBJECT_SAMPLE_FACTORS           	9029	9029-2-SA	Study_Diet:Modulen | Sex:Male | Time:Day 15	Type=Stool; Age=26; BMI=35.4; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0279_WUG_FARMM_HIL-9029-2-SA.raw 0279_WUG_FARMM_LIP-9029-2-SA.raw 0279_WU_FARMM_CMH_9029-2-SA.raw 0279_WUG_FARMM_FFA-9029-2-SA.raw
SUBJECT_SAMPLE_FACTORS           	9032	9032-2-SA	Study_Diet:Modulen | Sex:Male | Time:Day 15	Type=Stool; Age=53; BMI=31; Ethnicity=Not Hispanic or Latino; Race=White; RAW_FILE_NAME=0262_WUG_FARMM_HIL-9032-2-SA.raw 0262_WUG_FARMM_LIP-9032-2-SA.raw 0262_WU_FARMM_CMH_9032-2-SA.raw 0262_WUG_FARMM_FFA-9032-2-SA.raw
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS02	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=NA 0171a_WUG_FARMM_LIP-QCPS02.raw NA 0171a_WUG_FARMM_FFA-QCPS02.raw
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS03	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=NA 0191a_WUG_FARMM_LIP-QCPS03.raw NA 0191a_WUG_FARMM_FFA-QCPS03.raw
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS04	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=NA 0211a_WUG_FARMM_LIP-QCPS04.raw NA 0211a_WUG_FARMM_FFA-QCPS04.raw
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS05	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=NA 0231a_WUG_FARMM_LIP-QCPS05.raw NA 0231a_WUG_FARMM_FFA-QCPS05.raw
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS06	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=NA 0251a_WUG_FARMM_LIP-QCPS06.raw NA 0251a_WUG_FARMM_FFA-QCPS06.raw
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS07	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=NA 0271a_WUG_FARMM_LIP-QCPS07.raw NA 0271a_WUG_FARMM_FFA-QCPS07.raw
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS08	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=NA 0302a_WUG_FARMM_LIP-QCPS08.raw NA 0302a_WUG_FARMM_FFA-QCPS08.raw
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS09	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=NA 0302k_0302are_WUG_FARMM_LIP-QCPS09.raw NA NA
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS10	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=NA NA NA NA
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS11	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=0160a_WUG_FARMM_HIL-QPS11.raw NA 0150g_WU_FARMM_CMH_QPP-11.raw NA
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS12	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=0180a_WUG_FARMM_HIL-QPS12.raw NA 0171a_WU_FARMM_CMH_QPP-12.raw NA
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS13	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=0200a_WUG_FARMM_HIL-QPS13.raw NA 0191a_WU_FARMM_CMH_QPP-13.raw NA
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS14	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=0220a_WUG_FARMM_HIL-QPS14.raw NA 0211a_WU_FARMM_CMH_QPP-14.raw NA
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS15	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=0240a_WUG_FARMM_HIL-QPS15.raw NA 0231a_WU_FARMM_CMH_QPP-15.raw NA
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS16	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=0260a_WUG_FARMM_HIL-QPS16.raw NA 0231d_WU_FARMM_CMH_QPP-16.raw NA
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS17	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=0280a_WUG_FARMM_HIL-QPS17.raw NA 0251a_WU_FARMM_CMH_QPP-17.raw NA
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS18	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=0302a_WUG_FARMM_HIL-QPS18.raw NA 0271a_WU_FARMM_CMH_QPP-18.raw NA
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS19	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=NA NA 0291a_WU_FARMM_CMH_QPP-19.raw NA
SUBJECT_SAMPLE_FACTORS           	QC-pooled_stool	QCPS20	Study_Diet:NA | Sex:NA | Time:NA	Age=NA; Type=NA; BMI=NA; Ethnicity=NA; Race=NA; RAW_FILE_NAME=NA NA 0302a_WU_FARMM_CMH_QPP-20.raw NA
#COLLECTION
CO:COLLECTION_SUMMARY            	The first stool sample of each day of the inpatient groups was collected,
CO:COLLECTION_SUMMARY            	aliquoted and frozen immediately at -80oC. Blood was collected on days 1, 5, 9,
CO:COLLECTION_SUMMARY            	12 and 15 from which plasma aliquots were immediately isolated and frozen at
CO:COLLECTION_SUMMARY            	-80oC. The outpatient participants following the vegan diet also had blood
CO:COLLECTION_SUMMARY            	collected on days 1, 5, 9, 12, and 15 from which plasma aliquots were
CO:COLLECTION_SUMMARY            	immediately isolated and frozen at -80oC. Among these participants, the first
CO:COLLECTION_SUMMARY            	stool of the day was collected at home daily and kept on ice packs until it was
CO:COLLECTION_SUMMARY            	brought to the research unit where it could be aliquoted and frozen. Samples
CO:COLLECTION_SUMMARY            	from vegans were received for aliquoting within 24 hours and on average within 4
CO:COLLECTION_SUMMARY            	hours (Wu et al., 2010). Day 0 stool was not collected from vegans since their
CO:COLLECTION_SUMMARY            	diet did not change. The aliquoted amounts for all samples ranged from 500 mg to
CO:COLLECTION_SUMMARY            	1 g. The aliquots were taken from different areas of the sample. Remaining
CO:COLLECTION_SUMMARY            	sample was then collected into one residual 50 ml conical tube with a tongue
CO:COLLECTION_SUMMARY            	depressor. Any remaining stool was discarded. The University of Pennsylvania
CO:COLLECTION_SUMMARY            	Institutional Review Board (IRB) approved the research protocol.
CO:SAMPLE_TYPE                   	Feces
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	The 10 vegan participants continued to follow their usual diet as outpatients.
TR:TREATMENT_SUMMARY             	All participants completed the Diet History Questionnaire II (DHQ II), a food
TR:TREATMENT_SUMMARY             	frequency questionnaire developed by the Risk Factor Monitoring and Methods
TR:TREATMENT_SUMMARY             	Branch of the National Cancer Institute. The vegan participants also completed
TR:TREATMENT_SUMMARY             	three 24 hour diet recalls with a dietitian in the week prior to starting
TR:TREATMENT_SUMMARY             	antibiotics. 20 omnivores were randomly assigned to receive an omnivore diet or
TR:TREATMENT_SUMMARY             	EEN (Modulen® IBD) while residing in an inpatient research unit. The
TR:TREATMENT_SUMMARY             	macronutrient composition of Modulen® is protein 36g, fat 47g, and carbohydrate
TR:TREATMENT_SUMMARY             	110g per 1000 Kcal. The two omnivore diets were engineered to have a similar
TR:TREATMENT_SUMMARY             	composition to EEN. All the subjects consumed the menu A on day 11 and menu B on
TR:TREATMENT_SUMMARY             	days 4 and 14. Diets for omnivores were constructed to provide the expected
TR:TREATMENT_SUMMARY             	total calories required per day for the participant to maintain their current
TR:TREATMENT_SUMMARY             	weight and were adjusted if there was weight gain or loss of more than 2.5
TR:TREATMENT_SUMMARY             	pounds. On days 6, 7, and 8, inpatients participants received vancomycin 500mg
TR:TREATMENT_SUMMARY             	orally every 6 hours and neomycin 1000mg orally every 6 hours. On day 7,
TR:TREATMENT_SUMMARY             	participants consumed 4L of polyethylene glycol (PEG) based bowel purgative
TR:TREATMENT_SUMMARY             	(GoLytely®). Participants in the omnivore and EEN arms left the inpatient
TR:TREATMENT_SUMMARY             	research unit only under the direct supervision of a research staff member. The
TR:TREATMENT_SUMMARY             	vegan outpatients reported to the hospital twice on days 6,7 and 8 to receive
TR:TREATMENT_SUMMARY             	antibiotics and to consume PEG on day 7.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Stool samples (weight range 42.99-90.35 mg) were homogenized in 4 μL of water
SP:SAMPLEPREP_SUMMARY            	per milligram stool sample weight using a bead mill (TissueLyser II; Qiagen) and
SP:SAMPLEPREP_SUMMARY            	the aqueous homogenates were aliquoted for metabolite profiling analyses. Plasma
SP:SAMPLEPREP_SUMMARY            	samples were thawed and aliquoted for each LC-MS method. LC-MS samples were
SP:SAMPLEPREP_SUMMARY            	prepared for four profiling methods as follows: HILIC-pos: Metabolites were
SP:SAMPLEPREP_SUMMARY            	extracted by adding 90 μL of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic
SP:SAMPLEPREP_SUMMARY            	acid containing stable isotope-labeled internal standards (valine-d8, Isotec;
SP:SAMPLEPREP_SUMMARY            	and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA) to a 10 μL
SP:SAMPLEPREP_SUMMARY            	aliquot of stool homogenate or plasma. Samples were vortexed and then
SP:SAMPLEPREP_SUMMARY            	centrifuged (10 min, 9,000 x g, 4°C) to pellet protein precipitates.
SP:SAMPLEPREP_SUMMARY            	Supernatants were transferred to glass autosampler vials containing inserts for
SP:SAMPLEPREP_SUMMARY            	LC-MS analysis. C8-pos: Lipids were extracted by adding 190 μL of isopropanol
SP:SAMPLEPREP_SUMMARY            	containing 1-dodecanoyl-2-tridecanoyl-sn-glycero-3-phosphocholine as an internal
SP:SAMPLEPREP_SUMMARY            	standard (Avanti Polar Lipids; Alabaster, AL) to a 10 μL aliquot of stool
SP:SAMPLEPREP_SUMMARY            	homogenate or plasma. Samples were vortexed and then centrifuged (10 min, 9,000
SP:SAMPLEPREP_SUMMARY            	x g, ambient temperature) to pellet protein precipitates. Supernatants were
SP:SAMPLEPREP_SUMMARY            	transferred to glass autosampler vials containing inserts for LC-MS analysis.
SP:SAMPLEPREP_SUMMARY            	HILIC-neg: Metabolites were extracted by adding 120 μL of 80% methanol
SP:SAMPLEPREP_SUMMARY            	containing inosine-15N4, thymine-d4 and glycocholate-d4 internal standards
SP:SAMPLEPREP_SUMMARY            	(Cambridge Isotope Laboratories; Andover, MA) to a 30 μL aliquot of stool
SP:SAMPLEPREP_SUMMARY            	homogenate or plasma. Samples were vortexed and then centrifuged (10 min, 9,000
SP:SAMPLEPREP_SUMMARY            	x g, 4°C) to pellet protein precipitates. Supernatants were transferred to
SP:SAMPLEPREP_SUMMARY            	glass autosampler vials containing inserts for LC-MS analysis. C18-neg:
SP:SAMPLEPREP_SUMMARY            	Metabolites were extracted by adding 90 μL of methanol containing PGE2-d4 as an
SP:SAMPLEPREP_SUMMARY            	internal standard (Cayman Chemical Co.; Ann Arbor, MI) to a 30 μL aliquot of
SP:SAMPLEPREP_SUMMARY            	stool homogenate or plasma. Samples were vortexed and then centrifuged (10 min,
SP:SAMPLEPREP_SUMMARY            	9,000 x g, 4°C) to pellet protein precipitates. Supernatants were transferred
SP:SAMPLEPREP_SUMMARY            	to glass autosampler vials containing inserts for LC-MS analysis.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	HILIC-neg: high resolution and accurate mass profiling of polar metabolites
CH:CHROMATOGRAPHY_SUMMARY        	using HILIC and negative ion mode full scan MS
CH:INSTRUMENT_NAME               	Shimadzu Nexera X2
CH:COLUMN_NAME                   	Phenomenex Luna NH2 (150 x 2.1mm,3um)
CH:COLUMN_TEMPERATURE            	30 ℃
CH:FLOW_GRADIENT                 	linear
CH:FLOW_RATE                     	400 uL/min
CH:INJECTION_TEMPERATURE         	4
CH:SOLVENT_A                     	100% water; 20 mM ammonium acetate, 20 mM ammonium hydroxide
CH:SOLVENT_B                     	25% methanol/75% acetonitrile; 10 mM ammonium hydroxide
CH:CHROMATOGRAPHY_TYPE           	HILIC
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Plus Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:MS_COMMENTS                   	MS analyses were carried out using electrospray ionization in the negative ion
MS:MS_COMMENTS                   	mode using full scan analysis over m/z 60-750 at 70,000 resolution and 3 Hz data
MS:MS_COMMENTS                   	acquisition rate. Additional MS settings were: ion spray voltage, -3.0 kV;
MS:MS_COMMENTS                   	capillary temperature, 350°C; probe heater temperature, 325°C; sheath gas, 55;
MS:MS_COMMENTS                   	auxiliary gas, 10; and S-lens RF level 40. All raw data were processed using
MS:MS_COMMENTS                   	Progenesis QI software (NonLinear Dynamics) for feature alignment, nontargeted
MS:MS_COMMENTS                   	signal detection, and signal integration. Targeted processing of a subset of
MS:MS_COMMENTS                   	known metabolites was conducted using TraceFinder software (Thermo Fisher
MS:MS_COMMENTS                   	Scientific; Waltham, MA). Compound identities were confirmed using reference
MS:MS_COMMENTS                   	standards.
MS:ION_MODE                      	NEGATIVE
MS:MS_RESULTS_FILE               	ST001520_AN002531_Results.txt	UNITS:peak area	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END