#METABOLOMICS WORKBENCH yli_20201218_082950 DATATRACK_ID:2365 STUDY_ID:ST001635 ANALYSIS_ID:AN002672 PROJECT_ID:000000
VERSION             	1
CREATED_ON             	December 22, 2020, 12:24 pm
#PROJECT
PR:PROJECT_TITLE                 	Higher Dietary Carbohydrates Detrimentally Impact Obesity-Associated Breast
PR:PROJECT_TITLE                 	Cancer Chemoresistance
PR:PROJECT_TYPE                  	C18 Reversed-Phase Broad Spectrum Metabolomics
PR:PROJECT_SUMMARY               	Epidemiologic and experimental studies have established that obesity is an
PR:PROJECT_SUMMARY               	important risk and/or prognostic factor for most cancer types, but the
PR:PROJECT_SUMMARY               	mechanisms underlying the obesity-cancer link have not been clearly elucidated.
PR:PROJECT_SUMMARY               	The goal of this project is to address questions on the mechanisms of (and
PR:PROJECT_SUMMARY               	potential solutions to) obesity that results directly from diet exposures and
PR:PROJECT_SUMMARY               	the associated chemotherapeutic resistance. In a mouse model of basal-like
PR:PROJECT_SUMMARY               	breast cancer (BLBC), we compared a high protein (HP) diet to a high
PR:PROJECT_SUMMARY               	carbohydrate plus high fat (HCHF) diet and determined their comparative impacts
PR:PROJECT_SUMMARY               	on body weight, fatty liver development, tumor growth acute chemotherapy
PR:PROJECT_SUMMARY               	response to doxorubicin, profiled 80 inflammatory markers and performed
PR:PROJECT_SUMMARY               	untargeted metabolomics. Briefly, female C57BL/6 mice were fed either the HP or
PR:PROJECT_SUMMARY               	HCHF diet for 15 weeks, orthotopically implanted with MMTV-Wnt-1 mammary cells
PR:PROJECT_SUMMARY               	for tumor formation and growth (up to six weeks), then treated with a single
PR:PROJECT_SUMMARY               	dose of doxorubicin (or saline vehicle control) for 24 hours prior to study
PR:PROJECT_SUMMARY               	completion. Mice were euthanized and biospecimens were collected at the
PR:PROJECT_SUMMARY               	following study endpoints: 1-baseline (following 1-week quarantine); 2-after the
PR:PROJECT_SUMMARY               	15 weeks of differential diet exposure; 3-after 5 weeks and 5.5 weeks of tumor
PR:PROJECT_SUMMARY               	growth; and 4-after 24 hours of treatment, corresponding to 6 weeks of tumor
PR:PROJECT_SUMMARY               	growth. Untargeted metabolomics analysis was performed by UPLC high resolution
PR:PROJECT_SUMMARY               	mass spectrometry (LCMS) on liver samples at the four study endpoints described
PR:PROJECT_SUMMARY               	above. Liver tissues were sectioned into three discrete lobes (right, left and
PR:PROJECT_SUMMARY               	median=caudate + quadrate) prior to analysis.
PR:INSTITUTE                     	University of North Carolina at Chapel Hill
PR:DEPARTMENT                    	Nutrition
PR:LABORATORY                    	Metabolomics and Exposome Laboratory, Nutrition Research Institute, UNC Chapel
PR:LABORATORY                    	Hill
PR:LAST_NAME                     	Stewart
PR:FIRST_NAME                    	Delisha
PR:ADDRESS                       	500 Laureate Way, Nutrition Research Institute, UNC Chapel Hill
PR:EMAIL                         	delisha_stewart@unc.edu
PR:PHONE                         	+1 (704) 250-5068
#STUDY
ST:STUDY_TITLE                   	Higher Dietary Carbohydrates Detrimentally Impact Obesity-Associated Breast
ST:STUDY_TITLE                   	Cancer Chemoresistance
ST:STUDY_TYPE                    	Untargeted UPLC-MS Metabolomics Analysis
ST:STUDY_SUMMARY                 	This study is a part of an ongoing project entitled, “Higher Dietary
ST:STUDY_SUMMARY                 	Carbohydrates Detrimentally Impact Obesity-Associated Breast Cancer
ST:STUDY_SUMMARY                 	Chemoresistance.” (See project). Untargeted LCMS metabolomics analysis was
ST:STUDY_SUMMARY                 	performed to identify metabolic markers of nutritionally dependent and
ST:STUDY_SUMMARY                 	obesity-associated chemoresistance in female C57LB/6 mice fed either a high
ST:STUDY_SUMMARY                 	carbohydrate plus high fat (HCHF) diet or a high protein diet (HP). Mice were
ST:STUDY_SUMMARY                 	fed 15 weeks on either diet, then injected with a MMTV-Wnt-1 mouse mammary
ST:STUDY_SUMMARY                 	basal-like breast cancer cell line, for tumor formation up to 6 weeks. Three
ST:STUDY_SUMMARY                 	mice on either diet were treated for 24 hr with Doxorubicin or Saline as a
ST:STUDY_SUMMARY                 	vehicle control. All samples were analyzed in the positive mode for untargeted
ST:STUDY_SUMMARY                 	metabolomics analysis. Liver samples were selected for analysis because of an
ST:STUDY_SUMMARY                 	observation of fatty liver development in mice only on the HCHF diet after 15
ST:STUDY_SUMMARY                 	weeks. Samples were extracted and analyzed by UPLS-MS analysis using Q-Exactive
ST:STUDY_SUMMARY                 	HFX mass spectrometer.
ST:INSTITUTE                     	University of North Carolina at Chapel Hill
ST:DEPARTMENT                    	Nutrition
ST:LABORATORY                    	Metabolomics and Exposome Laboratory, Nutrition Research Institute, UNC Chapel
ST:LABORATORY                    	Hill
ST:LAST_NAME                     	Sumner
ST:FIRST_NAME                    	Susan
ST:ADDRESS                       	500 Laureate Way, Nutrition Research Institute, UNC Chapel Hill
ST:EMAIL                         	susan_sumner@unc.edu
ST:PHONE                         	(919) 622-4456
ST:NUM_GROUPS                    	6
ST:TOTAL_SUBJECTS                	193
ST:NUM_FEMALES                   	175
ST:STUDY_COMMENTS                	The number of groups includes the QC samples
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENOTYPE_STRAIN               	C57BL/6
SU:GENDER                        	Female
SU:ANIMAL_ANIMAL_SUPPLIER        	Charles River Labs
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	L_1	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_1.raw
SUBJECT_SAMPLE_FACTORS           	-	L_2	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_2.raw
SUBJECT_SAMPLE_FACTORS           	-	L_3	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_3.raw
SUBJECT_SAMPLE_FACTORS           	-	L_4	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_4.raw
SUBJECT_SAMPLE_FACTORS           	-	L_5	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_5.raw
SUBJECT_SAMPLE_FACTORS           	-	L_6	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_6.raw
SUBJECT_SAMPLE_FACTORS           	-	L_7	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_7.raw
SUBJECT_SAMPLE_FACTORS           	-	L_8	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_8.raw
SUBJECT_SAMPLE_FACTORS           	-	L_9	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_9.raw
SUBJECT_SAMPLE_FACTORS           	-	L_10	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_10.raw
SUBJECT_SAMPLE_FACTORS           	-	L_11	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_11.raw
SUBJECT_SAMPLE_FACTORS           	-	L_12	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_12.raw
SUBJECT_SAMPLE_FACTORS           	-	L_13	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_13.raw
SUBJECT_SAMPLE_FACTORS           	-	L_14	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_14.raw
SUBJECT_SAMPLE_FACTORS           	-	L_15	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_15.raw
SUBJECT_SAMPLE_FACTORS           	-	L_16	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_16.raw
SUBJECT_SAMPLE_FACTORS           	-	L_17	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_17.raw
SUBJECT_SAMPLE_FACTORS           	-	L_18	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_18.raw
SUBJECT_SAMPLE_FACTORS           	-	L_19	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_19.raw
SUBJECT_SAMPLE_FACTORS           	-	L_20	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_20.raw
SUBJECT_SAMPLE_FACTORS           	-	L_21	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_21.raw
SUBJECT_SAMPLE_FACTORS           	-	L_22	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_22.raw
SUBJECT_SAMPLE_FACTORS           	-	L_23	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_23.raw
SUBJECT_SAMPLE_FACTORS           	-	L_24	Diet:HP | Study Phenotype:Baseline | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_24.raw
SUBJECT_SAMPLE_FACTORS           	-	L_25	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_25.raw
SUBJECT_SAMPLE_FACTORS           	-	L_26	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_26.raw
SUBJECT_SAMPLE_FACTORS           	-	L_27	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_27.raw
SUBJECT_SAMPLE_FACTORS           	-	L_28	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_28.raw
SUBJECT_SAMPLE_FACTORS           	-	L_29	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_29.raw
SUBJECT_SAMPLE_FACTORS           	-	L_30	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_30.raw
SUBJECT_SAMPLE_FACTORS           	-	L_31	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_31.raw
SUBJECT_SAMPLE_FACTORS           	-	L_32	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_32.raw
SUBJECT_SAMPLE_FACTORS           	-	L_33	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_33.raw
SUBJECT_SAMPLE_FACTORS           	-	L_34	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_34.raw
SUBJECT_SAMPLE_FACTORS           	-	L_35	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_35.raw
SUBJECT_SAMPLE_FACTORS           	-	L_36	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_36.raw
SUBJECT_SAMPLE_FACTORS           	-	L_37	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_37.raw
SUBJECT_SAMPLE_FACTORS           	-	L_38	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_38.raw
SUBJECT_SAMPLE_FACTORS           	-	L_39	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_39.raw
SUBJECT_SAMPLE_FACTORS           	-	L_40	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_40.raw
SUBJECT_SAMPLE_FACTORS           	-	L_41	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_41.raw
SUBJECT_SAMPLE_FACTORS           	-	L_42	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_42.raw
SUBJECT_SAMPLE_FACTORS           	-	L_43	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_43.raw
SUBJECT_SAMPLE_FACTORS           	-	L_44	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_44.raw
SUBJECT_SAMPLE_FACTORS           	-	L_45	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_45.raw
SUBJECT_SAMPLE_FACTORS           	-	L_46	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_46.raw
SUBJECT_SAMPLE_FACTORS           	-	L_47	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_47.raw
SUBJECT_SAMPLE_FACTORS           	-	L_48	Diet:HCHF | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_48.raw
SUBJECT_SAMPLE_FACTORS           	-	L_49	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_49.raw
SUBJECT_SAMPLE_FACTORS           	-	L_50	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_50.raw
SUBJECT_SAMPLE_FACTORS           	-	L_51	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_51.raw
SUBJECT_SAMPLE_FACTORS           	-	L_52	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_52.raw
SUBJECT_SAMPLE_FACTORS           	-	L_53	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_53.raw
SUBJECT_SAMPLE_FACTORS           	-	L_54	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_54.raw
SUBJECT_SAMPLE_FACTORS           	-	L_55	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_55.raw
SUBJECT_SAMPLE_FACTORS           	-	L_56	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_56.raw
SUBJECT_SAMPLE_FACTORS           	-	L_57	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_57.raw
SUBJECT_SAMPLE_FACTORS           	-	L_58	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_58.raw
SUBJECT_SAMPLE_FACTORS           	-	L_59	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_59.raw
SUBJECT_SAMPLE_FACTORS           	-	L_60	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_60.raw
SUBJECT_SAMPLE_FACTORS           	-	L_61	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_61.raw
SUBJECT_SAMPLE_FACTORS           	-	L_62	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_62.raw
SUBJECT_SAMPLE_FACTORS           	-	L_63	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_63.raw
SUBJECT_SAMPLE_FACTORS           	-	L_64	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_64.raw
SUBJECT_SAMPLE_FACTORS           	-	L_65	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_65.raw
SUBJECT_SAMPLE_FACTORS           	-	L_66	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_66.raw
SUBJECT_SAMPLE_FACTORS           	-	L_67	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_67.raw
SUBJECT_SAMPLE_FACTORS           	-	L_68	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_68.raw
SUBJECT_SAMPLE_FACTORS           	-	L_69	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_69.raw
SUBJECT_SAMPLE_FACTORS           	-	L_70	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Right	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_70.raw
SUBJECT_SAMPLE_FACTORS           	-	L_71	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Left	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_71.raw
SUBJECT_SAMPLE_FACTORS           	-	L_72	Diet:HP | Study Phenotype:15 wk Differential Diet | Treatment:None | Liver Lobe:Median	Cell Line Injected=None; Sample Type=Sample; RAW_FILE_NAME=L_72.raw
SUBJECT_SAMPLE_FACTORS           	-	L_73	Diet:HCHF | Study Phenotype:Tumor Growth-5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_73.raw
SUBJECT_SAMPLE_FACTORS           	-	L_74	Diet:HCHF | Study Phenotype:Tumor Growth-5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_74.raw
SUBJECT_SAMPLE_FACTORS           	-	L_75	Diet:HCHF | Study Phenotype:Tumor Growth-5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_75.raw
SUBJECT_SAMPLE_FACTORS           	-	L_76	Diet:HCHF | Study Phenotype:Tumor Growth-5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_76.raw
SUBJECT_SAMPLE_FACTORS           	-	L_77	Diet:HCHF | Study Phenotype:Tumor Growth-5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_77.raw
SUBJECT_SAMPLE_FACTORS           	-	L_78	Diet:HCHF | Study Phenotype:Tumor Growth-5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_78.raw
SUBJECT_SAMPLE_FACTORS           	-	L_79	Diet:HP | Study Phenotype:Tumor Growth-5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_79.raw
SUBJECT_SAMPLE_FACTORS           	-	L_80	Diet:HP | Study Phenotype:Tumor Growth-5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_80.raw
SUBJECT_SAMPLE_FACTORS           	-	L_81	Diet:HP | Study Phenotype:Tumor Growth-5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_81.raw
SUBJECT_SAMPLE_FACTORS           	-	L_82	Diet:HP | Study Phenotype:Tumor Growth-5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_82.raw
SUBJECT_SAMPLE_FACTORS           	-	L_83	Diet:HP | Study Phenotype:Tumor Growth-5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_83.raw
SUBJECT_SAMPLE_FACTORS           	-	L_84	Diet:HP | Study Phenotype:Tumor Growth-5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_84.raw
SUBJECT_SAMPLE_FACTORS           	-	L_85	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_85.raw
SUBJECT_SAMPLE_FACTORS           	-	L_86	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_86.raw
SUBJECT_SAMPLE_FACTORS           	-	L_87	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_87.raw
SUBJECT_SAMPLE_FACTORS           	-	L_88	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_88.raw
SUBJECT_SAMPLE_FACTORS           	-	L_89	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_89.raw
SUBJECT_SAMPLE_FACTORS           	-	L_90	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_90.raw
SUBJECT_SAMPLE_FACTORS           	-	L_91	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_91.raw
SUBJECT_SAMPLE_FACTORS           	-	L_92	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_92.raw
SUBJECT_SAMPLE_FACTORS           	-	L_93	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_93.raw
SUBJECT_SAMPLE_FACTORS           	-	L_94	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_94.raw
SUBJECT_SAMPLE_FACTORS           	-	L_95	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_95.raw
SUBJECT_SAMPLE_FACTORS           	-	L_96	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_96.raw
SUBJECT_SAMPLE_FACTORS           	-	L_97	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_97.raw
SUBJECT_SAMPLE_FACTORS           	-	L_98	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_98.raw
SUBJECT_SAMPLE_FACTORS           	-	L_99	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_99.raw
SUBJECT_SAMPLE_FACTORS           	-	L_100	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_100.raw
SUBJECT_SAMPLE_FACTORS           	-	L_101	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_101.raw
SUBJECT_SAMPLE_FACTORS           	-	L_103	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_103.raw
SUBJECT_SAMPLE_FACTORS           	-	L_104	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_104.raw
SUBJECT_SAMPLE_FACTORS           	-	L_105	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_105.raw
SUBJECT_SAMPLE_FACTORS           	-	L_106	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_106.raw
SUBJECT_SAMPLE_FACTORS           	-	L_107	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_107.raw
SUBJECT_SAMPLE_FACTORS           	-	L_108	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_108.raw
SUBJECT_SAMPLE_FACTORS           	-	L_109	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_109.raw
SUBJECT_SAMPLE_FACTORS           	-	L_110	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_110.raw
SUBJECT_SAMPLE_FACTORS           	-	L_111	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_111.raw
SUBJECT_SAMPLE_FACTORS           	-	L_112	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_112.raw
SUBJECT_SAMPLE_FACTORS           	-	L_113	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_113.raw
SUBJECT_SAMPLE_FACTORS           	-	L_114	Diet:HCHF | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_114.raw
SUBJECT_SAMPLE_FACTORS           	-	L_115	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_115.raw
SUBJECT_SAMPLE_FACTORS           	-	L_116	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_116.raw
SUBJECT_SAMPLE_FACTORS           	-	L_117	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_117.raw
SUBJECT_SAMPLE_FACTORS           	-	L_118	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_118.raw
SUBJECT_SAMPLE_FACTORS           	-	L_119	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_119.raw
SUBJECT_SAMPLE_FACTORS           	-	L_120	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_120.raw
SUBJECT_SAMPLE_FACTORS           	-	L_121	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_121.raw
SUBJECT_SAMPLE_FACTORS           	-	L_122	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_122.raw
SUBJECT_SAMPLE_FACTORS           	-	L_123	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_123.raw
SUBJECT_SAMPLE_FACTORS           	-	L_124	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_124.raw
SUBJECT_SAMPLE_FACTORS           	-	L_127	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_127.raw
SUBJECT_SAMPLE_FACTORS           	-	L_130	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_130.raw
SUBJECT_SAMPLE_FACTORS           	-	L_131	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_131.raw
SUBJECT_SAMPLE_FACTORS           	-	L_132	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_132.raw
SUBJECT_SAMPLE_FACTORS           	-	L_133	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_133.raw
SUBJECT_SAMPLE_FACTORS           	-	L_134	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_134.raw
SUBJECT_SAMPLE_FACTORS           	-	L_135	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_135.raw
SUBJECT_SAMPLE_FACTORS           	-	L_136	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_136.raw
SUBJECT_SAMPLE_FACTORS           	-	L_137	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_137.raw
SUBJECT_SAMPLE_FACTORS           	-	L_138	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_138.raw
SUBJECT_SAMPLE_FACTORS           	-	L_139	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_139.raw
SUBJECT_SAMPLE_FACTORS           	-	L_140	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_140.raw
SUBJECT_SAMPLE_FACTORS           	-	L_141	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_141.raw
SUBJECT_SAMPLE_FACTORS           	-	L_142	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_142.raw
SUBJECT_SAMPLE_FACTORS           	-	L_143	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_143.raw
SUBJECT_SAMPLE_FACTORS           	-	L_144	Diet:HP | Study Phenotype:Tumor Growth-5.5 wk | Treatment:None | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_144.raw
SUBJECT_SAMPLE_FACTORS           	-	L_145	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_145.raw
SUBJECT_SAMPLE_FACTORS           	-	L_146	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_146.raw
SUBJECT_SAMPLE_FACTORS           	-	L_147	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_147.raw
SUBJECT_SAMPLE_FACTORS           	-	L_148	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_148.raw
SUBJECT_SAMPLE_FACTORS           	-	L_149	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_149.raw
SUBJECT_SAMPLE_FACTORS           	-	L_150	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_150.raw
SUBJECT_SAMPLE_FACTORS           	-	L_151	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_151.raw
SUBJECT_SAMPLE_FACTORS           	-	L_152	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_152.raw
SUBJECT_SAMPLE_FACTORS           	-	L_153	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_153.raw
SUBJECT_SAMPLE_FACTORS           	-	L_154	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_154.raw
SUBJECT_SAMPLE_FACTORS           	-	L_155	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_155.raw
SUBJECT_SAMPLE_FACTORS           	-	L_156	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_156.raw
SUBJECT_SAMPLE_FACTORS           	-	L_157	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_157.raw
SUBJECT_SAMPLE_FACTORS           	-	L_158	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_158.raw
SUBJECT_SAMPLE_FACTORS           	-	L_159	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_159.raw
SUBJECT_SAMPLE_FACTORS           	-	L_160	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_160.raw
SUBJECT_SAMPLE_FACTORS           	-	L_161	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_161.raw
SUBJECT_SAMPLE_FACTORS           	-	L_162	Diet:HCHF | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_162.raw
SUBJECT_SAMPLE_FACTORS           	-	L_163	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_163.raw
SUBJECT_SAMPLE_FACTORS           	-	L_164	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_164.raw
SUBJECT_SAMPLE_FACTORS           	-	L_165	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_165.raw
SUBJECT_SAMPLE_FACTORS           	-	L_166	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_166.raw
SUBJECT_SAMPLE_FACTORS           	-	L_167	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_167.raw
SUBJECT_SAMPLE_FACTORS           	-	L_168	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_168.raw
SUBJECT_SAMPLE_FACTORS           	-	L_169	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_169.raw
SUBJECT_SAMPLE_FACTORS           	-	L_170	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_170.raw
SUBJECT_SAMPLE_FACTORS           	-	L_171	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:DOX-treated | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_171.raw
SUBJECT_SAMPLE_FACTORS           	-	L_172	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_172.raw
SUBJECT_SAMPLE_FACTORS           	-	L_173	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_173.raw
SUBJECT_SAMPLE_FACTORS           	-	L_174	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_174.raw
SUBJECT_SAMPLE_FACTORS           	-	L_175	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_175.raw
SUBJECT_SAMPLE_FACTORS           	-	L_176	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_176.raw
SUBJECT_SAMPLE_FACTORS           	-	L_177	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_177.raw
SUBJECT_SAMPLE_FACTORS           	-	L_178	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Right	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_178.raw
SUBJECT_SAMPLE_FACTORS           	-	L_179	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Left	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_179.raw
SUBJECT_SAMPLE_FACTORS           	-	L_180	Diet:HP | Study Phenotype:24 hr Treatment | Treatment:Saline-control | Liver Lobe:Median	Cell Line Injected=BLBC; Sample Type=Sample; RAW_FILE_NAME=L_180.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_1-4	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_1-4.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_2-1	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_2-1.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_2-2	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_2-2.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_3-1	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_3-1.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_3-2	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_3-2.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_4-1	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_4-1.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_4-2	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_4-2.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_5-1	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_5-1.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_5-2	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_5-2.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_6-4	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_6-4.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_7-1	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_7-1.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_7-2	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_7-2.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_8-1	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_8-1.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_8-2	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_8-2.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_9-1	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_9-1.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_9-2	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_9-2.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_10-1	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_10-1.raw
SUBJECT_SAMPLE_FACTORS           	-	SP_10-2	Diet:- | Study Phenotype:Pooled | Treatment:- | Liver Lobe:-	Cell Line Injected=-; Sample Type=QC; RAW_FILE_NAME=SP_10-2.raw
#COLLECTION
CO:COLLECTION_SUMMARY            	See the project foe more details. Briefly, mice were euthanized and biospecimens
CO:COLLECTION_SUMMARY            	were collected at the following study endpoints: 1-baseline (following 1-week
CO:COLLECTION_SUMMARY            	quarantine); 2-after the 15 weeks of differential diet exposure; 3-after 5 weeks
CO:COLLECTION_SUMMARY            	and 5.5 weeks of tumor growth; and 4-after 24 hours of treatment, corresponding
CO:COLLECTION_SUMMARY            	to 6 weeks of tumor growth.
CO:SAMPLE_TYPE                   	Liver
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	24 hr single dose treatment with Doxorubicin or saline (vehicle control). See
TR:TREATMENT_SUMMARY             	project for more details.
TR:TREATMENT_DOSE                	10 mg/kg
TR:TREATMENT_DOSEVOLUME          	100 uL
TR:TREATMENT_DOSEDURATION        	24 hr
TR:TREATMENT_VEHICLE             	saline
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Liver tissue samples were put into randomized order (n=60), then were weighed
SP:SAMPLEPREP_SUMMARY            	then sectioned into three lobes (on dry ice) prior to analyses. Tissues were
SP:SAMPLEPREP_SUMMARY            	sectioned into the Right lobe, Left lobe and Median lobe (comprised of the
SP:SAMPLEPREP_SUMMARY            	caudate+quadrate lobes). Individual lobe weights were measured, then each lobe
SP:SAMPLEPREP_SUMMARY            	was further sectioned into equal numbers of cubes (4-6 cubes/lobe), depending on
SP:SAMPLEPREP_SUMMARY            	the lobe size. Two-three smaller cubes, were recombined into a new tube, weighed
SP:SAMPLEPREP_SUMMARY            	and used for metabolomics analysis. Two mice (HP diet, 5.5 weeks of tumor
SP:SAMPLEPREP_SUMMARY            	growth) were missing both left and median lobes from their gross liver tissues,
SP:SAMPLEPREP_SUMMARY            	and one mouse (HCHF diet, 5.5 weeks of tumor growth) was missing the median
SP:SAMPLEPREP_SUMMARY            	lobe; thus these samples were not included for metabolomics analysis. Thus, the
SP:SAMPLEPREP_SUMMARY            	total number of experimental samples was 175 rather than 180.
SP:PROCESSING_METHOD             	Sectioned and weighed frozen liver tissue samples (n=175, 60-450 mg) were
SP:PROCESSING_METHOD             	homogenized with cold methanol (10 µl for every mg of tissue) in a Bead Ruptor
SP:PROCESSING_METHOD             	Elite Bead Mill Homogenizer (OMNI International) at 5.0 m/s for 30 s in two
SP:PROCESSING_METHOD             	cycles. The supernatants were collected after centrifugation at 16,000 rcf for
SP:PROCESSING_METHOD             	20 min at 4 °C. A quality control pool (QC pool) sample was prepared by pooling
SP:PROCESSING_METHOD             	30 µL supernatant aliquots from selected individual samples (n=78), with a
SP:PROCESSING_METHOD             	total pre-processing weight ≥ 60 mg. The supernatants (200 µL) of each study
SP:PROCESSING_METHOD             	sample and QC pools were transferred to new tubes and dried under Speed-vac.
SP:PROCESSING_METHOD             	Each dried sample was reconstituted in a 200 µL water-methanol (95:5) solution
SP:PROCESSING_METHOD             	containing 500 ng/ml L-tryphotophan-d5. The samples were thoroughly mixed on
SP:PROCESSING_METHOD             	multiple tube vortexer for 10 mins at 5000 rpm. Samples were centrifuged at room
SP:PROCESSING_METHOD             	temperature and at 16,000 rcf for 4 min. The supernatant was transferred to
SP:PROCESSING_METHOD             	pre-labeled auto-sampler vials for data acquisition. Untargeted metabolomics was
SP:PROCESSING_METHOD             	conducted using a Thermo Scientific™ Vanquish™ UPHPLC - Q Exactive™ HF-X
SP:PROCESSING_METHOD             	Orbitrap System. Metabolites were separated on an Acquity UPLC HSS T3 C18 (2.1 X
SP:PROCESSING_METHOD             	100 mm, 1.8 µm) operating at 50 °C using a reversed phase gradient separation
SP:PROCESSING_METHOD             	with Water with 0.1% Formic Acid (v/v) as mobile phase A and Methanol with 0.1%
SP:PROCESSING_METHOD             	Formic Acid (v/v) as mobile phase B. A 5 µL amount was injected into the
SP:PROCESSING_METHOD             	instrument, and MS data was collected between 50-750 m/z in the positive mode
SP:PROCESSING_METHOD             	with the MS/MS data triggered by the Data-Dependent Acquisition.
SP:PROCESSING_STORAGE_CONDITIONS 	4℃
SP:EXTRACT_STORAGE               	-80℃
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	None
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	Waters Acquity BEH HSS T3 (100 x 2.1mm, 1.8um)
CH:FLOW_RATE                     	0.4 mL/min
CH:COLUMN_TEMPERATURE            	8
CH:SOLVENT_A                     	Water with 0.1% Formic Acid
CH:SOLVENT_B                     	Methanol with 0.1% Formic Acid
CH:COLUMN_PRESSURE               	6000-10000
CH:INJECTION_TEMPERATURE         	8
CH:WEAK_WASH_SOLVENT_NAME        	10:90 Methanol:Water with 0.1% FA solution
CH:STRONG_WASH_SOLVENT_NAME      	75:25 2-Propanol: Water with 0.1% FA solution
CH:RANDOMIZATION_ORDER           	Yes
CH:CHROMATOGRAPHY_COMMENTS       	Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3.
CH:CHROMATOGRAPHY_COMMENTS       	16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:LABORATORY_NAME               	UNC-NRI Sumner lab
AN:OPERATOR_NAME                 	Yuanyuan Li
AN:DETECTOR_TYPE                 	Orbitrap
AN:SOFTWARE_VERSION              	Xcalibur 4.1
AN:DATA_FORMAT                   	profile
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive HF hybrid Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	NA
MS:CAPILLARY_TEMPERATURE         	275 °C
MS:CAPILLARY_VOLTAGE             	3.5 KV
MS:COLLISION_ENERGY              	10-35, ramp
MS:COLLISION_GAS                 	N2
MS:DRY_GAS_FLOW                  	45
MS:DRY_GAS_TEMP                  	325°C
MS:FRAGMENTATION_METHOD          	CID
MS:DESOLVATION_GAS_FLOW          	45
MS:MS_RESULTS_FILE               	ST001635_AN002672_Results.txt	UNITS:peak area	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END