#METABOLOMICS WORKBENCH wpathmasiri_20210302_074234 DATATRACK_ID:2515 STUDY_ID:ST001716 ANALYSIS_ID:AN002796 PROJECT_ID:PR001100 VERSION 1 CREATED_ON March 2, 2021, 9:49 am #PROJECT PR:PROJECT_TITLE Metabolomics in the New Hampshire Birth Cohort Study PR:PROJECT_TYPE Metabolomics Analysis PR:PROJECT_SUMMARY Data were obtained from participants in the New Hampshire Birth Cohort Study, a PR:PROJECT_SUMMARY prospective pregnancy cohort. Chemical co-exposures were assessed via silicone PR:PROJECT_SUMMARY wristbands worn for one week at approximately 13 gestational weeks. Metabolomic PR:PROJECT_SUMMARY features were assessed in maternal plasma samples obtained at ~24-28 gestational PR:PROJECT_SUMMARY weeks via the Biocrates AbsoluteIDQ® p180 kit and nuclear magnetic resonance PR:PROJECT_SUMMARY (NMR) spectroscopy. Associations between chemical co-exposures and plasma PR:PROJECT_SUMMARY metabolomics were investigated using multivariate modeling to examine the PR:PROJECT_SUMMARY association of chemical co-exposures on the maternal plasma metabolome during PR:PROJECT_SUMMARY pregnancy. PR:INSTITUTE Dartmouth College PR:DEPARTMENT Department of Epidemiology PR:LAST_NAME Karagas PR:FIRST_NAME Margaret PR:ADDRESS Geisel School of Medicine at Dartmouth, 1 Medical Center Drive, Williamson PR:ADDRESS Translational Research Building, 7th Floor, Lebanon, NH 03756 PR:EMAIL margaret.r.karagas@dartmouth.edu PR:PHONE 919-541-6861 PR:FUNDING_SOURCE NIH, Office of the Director, UH3 OD023275, NIH NIEHS P42ES007373, and NIH NIGMS PR:FUNDING_SOURCE P20 GM104416. This project was supported by the Opportunities and Infrastructure PR:FUNDING_SOURCE Fund of the ECHO Program (EC0156; OIF PI: Romano, Cohort PI: Karagas PR:PROJECT_COMMENTS For further details, contact Margaret Karagass for information on the New PR:PROJECT_COMMENTS Hampshire Birth Cohort Study #STUDY ST:STUDY_TITLE Chemical co-exposures assessed via silicone wristbands and endogenous plasma ST:STUDY_TITLE metabolomics during pregnancy ST:STUDY_TYPE Untargeted Metabolomics Analysis ST:STUDY_SUMMARY Metabolomics analysis was performed on maternal plasma samples obtained from the ST:STUDY_SUMMARY subjets in the New Hampshire Birth Cohort Study ST:INSTITUTE University of North Carolina at Chapel Hill ST:DEPARTMENT Nutrition ST:LABORATORY Metabolomics and Exposome Laboratory at UNC Nutrition Research Institute ST:LAST_NAME Sumner ST:FIRST_NAME Susan ST:ADDRESS 500 Laureate Way, Kannapolis, NC, 28081, USA ST:EMAIL Wimal_pathmasiri@unc.edu ST:PHONE 919-622-4456 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - CP_101 phenotype:QC | nmr_batch:Batch 10 RAW_FILE_NAME=CP_101 SUBJECT_SAMPLE_FACTORS - CP_102 phenotype:QC | nmr_batch:Batch 10 RAW_FILE_NAME=CP_102 SUBJECT_SAMPLE_FACTORS - CP_103 phenotype:QC | nmr_batch:Batch 10 RAW_FILE_NAME=CP_103 SUBJECT_SAMPLE_FACTORS - CP_104 phenotype:QC | nmr_batch:Batch 10 RAW_FILE_NAME=CP_104 SUBJECT_SAMPLE_FACTORS - CP_105 phenotype:QC | nmr_batch:Batch 10 RAW_FILE_NAME=CP_105 SUBJECT_SAMPLE_FACTORS - CP_106 phenotype:QC | nmr_batch:Batch 10 RAW_FILE_NAME=CP_106 SUBJECT_SAMPLE_FACTORS - CP_107 phenotype:QC | nmr_batch:Batch 10 RAW_FILE_NAME=CP_107 SUBJECT_SAMPLE_FACTORS - CP_108 phenotype:QC | nmr_batch:Batch 10 RAW_FILE_NAME=CP_108 SUBJECT_SAMPLE_FACTORS - CP_109 phenotype:QC | nmr_batch:Batch 10 RAW_FILE_NAME=CP_109 SUBJECT_SAMPLE_FACTORS - CP_111 phenotype:QC | nmr_batch:Batch 11 RAW_FILE_NAME=CP_111 SUBJECT_SAMPLE_FACTORS - CP_112 phenotype:QC | nmr_batch:Batch 11 RAW_FILE_NAME=CP_112 SUBJECT_SAMPLE_FACTORS - CP_114 phenotype:QC | nmr_batch:Batch 11 RAW_FILE_NAME=CP_114 SUBJECT_SAMPLE_FACTORS - CP_115 phenotype:QC | nmr_batch:Batch 11 RAW_FILE_NAME=CP_115 SUBJECT_SAMPLE_FACTORS - CP_116 phenotype:QC | nmr_batch:Batch 11 RAW_FILE_NAME=CP_116 SUBJECT_SAMPLE_FACTORS - CP_117 phenotype:QC | nmr_batch:Batch 11 RAW_FILE_NAME=CP_117 SUBJECT_SAMPLE_FACTORS - CP_118 phenotype:QC | nmr_batch:Batch 11 RAW_FILE_NAME=CP_118 SUBJECT_SAMPLE_FACTORS - CP_161 phenotype:QC | nmr_batch:Batch 16 RAW_FILE_NAME=CP_161 SUBJECT_SAMPLE_FACTORS - CP_162 phenotype:QC | nmr_batch:Batch 16 RAW_FILE_NAME=CP_162 SUBJECT_SAMPLE_FACTORS - CP_163 phenotype:QC | nmr_batch:Batch 16 RAW_FILE_NAME=CP_163 SUBJECT_SAMPLE_FACTORS - CP_164 phenotype:QC | nmr_batch:Batch 16 RAW_FILE_NAME=CP_164 SUBJECT_SAMPLE_FACTORS - CP_165 phenotype:QC | nmr_batch:Batch 16 RAW_FILE_NAME=CP_165 SUBJECT_SAMPLE_FACTORS - CP_166 phenotype:QC | nmr_batch:Batch 16 RAW_FILE_NAME=CP_166 SUBJECT_SAMPLE_FACTORS - CP_171 phenotype:QC | nmr_batch:Batch 17 RAW_FILE_NAME=CP_171 SUBJECT_SAMPLE_FACTORS - CP_172 phenotype:QC | nmr_batch:Batch 17 RAW_FILE_NAME=CP_172 SUBJECT_SAMPLE_FACTORS - CP_173 phenotype:QC | nmr_batch:Batch 17 RAW_FILE_NAME=CP_173 SUBJECT_SAMPLE_FACTORS - CP_174 phenotype:QC | nmr_batch:Batch 17 RAW_FILE_NAME=CP_174 SUBJECT_SAMPLE_FACTORS - S_820 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_820 SUBJECT_SAMPLE_FACTORS - S_821 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_821 SUBJECT_SAMPLE_FACTORS - S_823 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_823 SUBJECT_SAMPLE_FACTORS - S_827 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_827 SUBJECT_SAMPLE_FACTORS - S_828 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_828 SUBJECT_SAMPLE_FACTORS - S_829 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_829 SUBJECT_SAMPLE_FACTORS - S_831 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_831 SUBJECT_SAMPLE_FACTORS - S_832 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_832 SUBJECT_SAMPLE_FACTORS - S_834 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_834 SUBJECT_SAMPLE_FACTORS - S_838 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_838 SUBJECT_SAMPLE_FACTORS - S_839 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_839 SUBJECT_SAMPLE_FACTORS - S_841 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_841 SUBJECT_SAMPLE_FACTORS - S_843 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_843 SUBJECT_SAMPLE_FACTORS - S_844 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_844 SUBJECT_SAMPLE_FACTORS - S_845 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_845 SUBJECT_SAMPLE_FACTORS - S_846 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_846 SUBJECT_SAMPLE_FACTORS - S_847 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_847 SUBJECT_SAMPLE_FACTORS - S_848 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_848 SUBJECT_SAMPLE_FACTORS - S_850 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_850 SUBJECT_SAMPLE_FACTORS - S_851 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_851 SUBJECT_SAMPLE_FACTORS - S_852 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_852 SUBJECT_SAMPLE_FACTORS - S_853 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_853 SUBJECT_SAMPLE_FACTORS - S_854 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_854 SUBJECT_SAMPLE_FACTORS - S_856 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_856 SUBJECT_SAMPLE_FACTORS - S_857 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_857 SUBJECT_SAMPLE_FACTORS - S_858 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_858 SUBJECT_SAMPLE_FACTORS - S_859 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_859 SUBJECT_SAMPLE_FACTORS - S_860 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_860 SUBJECT_SAMPLE_FACTORS - S_861 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_861 SUBJECT_SAMPLE_FACTORS - S_862 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_862 SUBJECT_SAMPLE_FACTORS - S_864 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_864 SUBJECT_SAMPLE_FACTORS - S_865 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_865 SUBJECT_SAMPLE_FACTORS - S_866 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_866 SUBJECT_SAMPLE_FACTORS - S_867 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_867 SUBJECT_SAMPLE_FACTORS - S_868 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_868 SUBJECT_SAMPLE_FACTORS - S_869 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_869 SUBJECT_SAMPLE_FACTORS - S_870 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_870 SUBJECT_SAMPLE_FACTORS - S_871 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_871 SUBJECT_SAMPLE_FACTORS - S_872 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_872 SUBJECT_SAMPLE_FACTORS - S_873 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_873 SUBJECT_SAMPLE_FACTORS - S_874 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_874 SUBJECT_SAMPLE_FACTORS - S_875 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_875 SUBJECT_SAMPLE_FACTORS - S_880 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_880 SUBJECT_SAMPLE_FACTORS - S_883 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_883 SUBJECT_SAMPLE_FACTORS - S_885 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_885 SUBJECT_SAMPLE_FACTORS - S_886 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_886 SUBJECT_SAMPLE_FACTORS - S_887 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_887 SUBJECT_SAMPLE_FACTORS - S_888 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_888 SUBJECT_SAMPLE_FACTORS - S_889 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_889 SUBJECT_SAMPLE_FACTORS - S_891 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_891 SUBJECT_SAMPLE_FACTORS - S_892 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_892 SUBJECT_SAMPLE_FACTORS - S_894 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_894 SUBJECT_SAMPLE_FACTORS - S_895 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_895 SUBJECT_SAMPLE_FACTORS - S_897 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_897 SUBJECT_SAMPLE_FACTORS - S_898 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_898 SUBJECT_SAMPLE_FACTORS - S_901 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_901 SUBJECT_SAMPLE_FACTORS - S_902 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_902 SUBJECT_SAMPLE_FACTORS - S_906 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_906 SUBJECT_SAMPLE_FACTORS - S_907 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_907 SUBJECT_SAMPLE_FACTORS - S_909 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_909 SUBJECT_SAMPLE_FACTORS - S_910 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_910 SUBJECT_SAMPLE_FACTORS - S_912 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_912 SUBJECT_SAMPLE_FACTORS - S_913 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_913 SUBJECT_SAMPLE_FACTORS - S_915 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_915 SUBJECT_SAMPLE_FACTORS - S_916 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_916 SUBJECT_SAMPLE_FACTORS - S_917 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_917 SUBJECT_SAMPLE_FACTORS - S_918 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_918 SUBJECT_SAMPLE_FACTORS - S_919 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_919 SUBJECT_SAMPLE_FACTORS - S_920 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_920 SUBJECT_SAMPLE_FACTORS - S_921 phenotype:Maternal | nmr_batch:Batch 10 RAW_FILE_NAME=S_921 SUBJECT_SAMPLE_FACTORS - S_819 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_819 SUBJECT_SAMPLE_FACTORS - S_825 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_825 SUBJECT_SAMPLE_FACTORS - S_826 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_826 SUBJECT_SAMPLE_FACTORS - S_830 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_830 SUBJECT_SAMPLE_FACTORS - S_833 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_833 SUBJECT_SAMPLE_FACTORS - S_835 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_835 SUBJECT_SAMPLE_FACTORS - S_836 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_836 SUBJECT_SAMPLE_FACTORS - S_840 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_840 SUBJECT_SAMPLE_FACTORS - S_849 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_849 SUBJECT_SAMPLE_FACTORS - S_855 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_855 SUBJECT_SAMPLE_FACTORS - S_863 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_863 SUBJECT_SAMPLE_FACTORS - S_876 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_876 SUBJECT_SAMPLE_FACTORS - S_877 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_877 SUBJECT_SAMPLE_FACTORS - S_879 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_879 SUBJECT_SAMPLE_FACTORS - S_881 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_881 SUBJECT_SAMPLE_FACTORS - S_893 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_893 SUBJECT_SAMPLE_FACTORS - S_899 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_899 SUBJECT_SAMPLE_FACTORS - S_900 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_900 SUBJECT_SAMPLE_FACTORS - S_903 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_903 SUBJECT_SAMPLE_FACTORS - S_904 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_904 SUBJECT_SAMPLE_FACTORS - S_922 phenotype:Maternal | nmr_batch:Batch 11 RAW_FILE_NAME=S_922 SUBJECT_SAMPLE_FACTORS - S_1322 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1322 SUBJECT_SAMPLE_FACTORS - S_1323 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1323 SUBJECT_SAMPLE_FACTORS - S_1324 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1324 SUBJECT_SAMPLE_FACTORS - S_1325 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1325 SUBJECT_SAMPLE_FACTORS - S_1326 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1326 SUBJECT_SAMPLE_FACTORS - S_1328 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1328 SUBJECT_SAMPLE_FACTORS - S_1330 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1330 SUBJECT_SAMPLE_FACTORS - S_1331 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1331 SUBJECT_SAMPLE_FACTORS - S_1332 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1332 SUBJECT_SAMPLE_FACTORS - S_1333 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1333 SUBJECT_SAMPLE_FACTORS - S_1334 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1334 SUBJECT_SAMPLE_FACTORS - S_1335 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1335 SUBJECT_SAMPLE_FACTORS - S_1336 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1336 SUBJECT_SAMPLE_FACTORS - S_1338 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1338 SUBJECT_SAMPLE_FACTORS - S_1339 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1339 SUBJECT_SAMPLE_FACTORS - S_1340 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1340 SUBJECT_SAMPLE_FACTORS - S_1341 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1341 SUBJECT_SAMPLE_FACTORS - S_1342 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1342 SUBJECT_SAMPLE_FACTORS - S_1343 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1343 SUBJECT_SAMPLE_FACTORS - S_1345 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1345 SUBJECT_SAMPLE_FACTORS - S_1348 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1348 SUBJECT_SAMPLE_FACTORS - S_1349 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1349 SUBJECT_SAMPLE_FACTORS - S_1350 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1350 SUBJECT_SAMPLE_FACTORS - S_1351 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1351 SUBJECT_SAMPLE_FACTORS - S_1353 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1353 SUBJECT_SAMPLE_FACTORS - S_1354 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1354 SUBJECT_SAMPLE_FACTORS - S_1356 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1356 SUBJECT_SAMPLE_FACTORS - S_1357 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1357 SUBJECT_SAMPLE_FACTORS - S_1358 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1358 SUBJECT_SAMPLE_FACTORS - S_1359 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1359 SUBJECT_SAMPLE_FACTORS - S_1360 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1360 SUBJECT_SAMPLE_FACTORS - S_1361 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1361 SUBJECT_SAMPLE_FACTORS - S_1363 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1363 SUBJECT_SAMPLE_FACTORS - S_1366 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1366 SUBJECT_SAMPLE_FACTORS - S_1367 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1367 SUBJECT_SAMPLE_FACTORS - S_1368 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1368 SUBJECT_SAMPLE_FACTORS - S_1369 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1369 SUBJECT_SAMPLE_FACTORS - S_1371 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1371 SUBJECT_SAMPLE_FACTORS - S_1373 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1373 SUBJECT_SAMPLE_FACTORS - S_1375 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1375 SUBJECT_SAMPLE_FACTORS - S_1376 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1376 SUBJECT_SAMPLE_FACTORS - S_1377 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1377 SUBJECT_SAMPLE_FACTORS - S_1378 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1378 SUBJECT_SAMPLE_FACTORS - S_1379 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1379 SUBJECT_SAMPLE_FACTORS - S_1381 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1381 SUBJECT_SAMPLE_FACTORS - S_1383 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1383 SUBJECT_SAMPLE_FACTORS - S_1384 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1384 SUBJECT_SAMPLE_FACTORS - S_1385 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1385 SUBJECT_SAMPLE_FACTORS - S_1387 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1387 SUBJECT_SAMPLE_FACTORS - S_1388 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1388 SUBJECT_SAMPLE_FACTORS - S_1389 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1389 SUBJECT_SAMPLE_FACTORS - S_1390 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1390 SUBJECT_SAMPLE_FACTORS - S_1391 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1391 SUBJECT_SAMPLE_FACTORS - S_1393 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1393 SUBJECT_SAMPLE_FACTORS - S_1394 phenotype:Maternal | nmr_batch:Batch 16 RAW_FILE_NAME=S_1394 SUBJECT_SAMPLE_FACTORS - S_1396 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1396 SUBJECT_SAMPLE_FACTORS - S_1397 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1397 SUBJECT_SAMPLE_FACTORS - S_1398 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1398 SUBJECT_SAMPLE_FACTORS - S_1400 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1400 SUBJECT_SAMPLE_FACTORS - S_1401 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1401 SUBJECT_SAMPLE_FACTORS - S_1403 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1403 SUBJECT_SAMPLE_FACTORS - S_1405 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1405 SUBJECT_SAMPLE_FACTORS - S_1406 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1406 SUBJECT_SAMPLE_FACTORS - S_1407 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1407 SUBJECT_SAMPLE_FACTORS - S_1408 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1408 SUBJECT_SAMPLE_FACTORS - S_1409 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1409 SUBJECT_SAMPLE_FACTORS - S_1410 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1410 SUBJECT_SAMPLE_FACTORS - S_1411 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1411 SUBJECT_SAMPLE_FACTORS - S_1414 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1414 SUBJECT_SAMPLE_FACTORS - S_1416 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1416 SUBJECT_SAMPLE_FACTORS - S_1417 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1417 SUBJECT_SAMPLE_FACTORS - S_1419 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1419 SUBJECT_SAMPLE_FACTORS - S_1420 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1420 SUBJECT_SAMPLE_FACTORS - S_1422 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1422 SUBJECT_SAMPLE_FACTORS - S_1423 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1423 SUBJECT_SAMPLE_FACTORS - S_1424 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1424 SUBJECT_SAMPLE_FACTORS - S_1425 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1425 SUBJECT_SAMPLE_FACTORS - S_1426 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1426 SUBJECT_SAMPLE_FACTORS - S_1427 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1427 SUBJECT_SAMPLE_FACTORS - S_1428 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1428 SUBJECT_SAMPLE_FACTORS - S_1429 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1429 SUBJECT_SAMPLE_FACTORS - S_1431 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1431 SUBJECT_SAMPLE_FACTORS - S_1432 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1432 SUBJECT_SAMPLE_FACTORS - S_1433 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1433 SUBJECT_SAMPLE_FACTORS - S_1434 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1434 SUBJECT_SAMPLE_FACTORS - S_1435 phenotype:Maternal | nmr_batch:Batch 17 RAW_FILE_NAME=S_1435 #COLLECTION CO:COLLECTION_SUMMARY At the mother’s clinical prenatal blood draw at 24-28 gestational weeks CO:COLLECTION_SUMMARY (mean=28 week; range 20-38 weeks), the New Hampshire Birth Cohort Study requests CO:COLLECTION_SUMMARY and processes a small sample of blood, using a 6 ml potassium K2 EDTA 10.8 mg CO:COLLECTION_SUMMARY royal blue top tube. Plasma samples are stored at -80C. CO:SAMPLE_TYPE Blood (plasma) CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY N/A #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Aliquots of each de-identified sample were received by the NIH ERCMRC on dry ice SP:SAMPLEPREP_SUMMARY and immediately stored at -80 °C after being logged in for metabolomics SP:SAMPLEPREP_SUMMARY analysis. The samples were randomized into NMR batches for processing and data SP:SAMPLEPREP_SUMMARY acquisition. Samples of each NMR batch was prepared as follows. The study plasma SP:SAMPLEPREP_SUMMARY samples were thawed at 4°C overnight. An aliquot (350 µL) of thawed plasma was SP:SAMPLEPREP_SUMMARY transferred into labeled 2.0 mL LoBind Eppendorf tubes. Low volume samples that SP:SAMPLEPREP_SUMMARY were below 350 µL were made up to 350 µL with water. A volume of 1050 µL of SP:SAMPLEPREP_SUMMARY Methanol was added to each sample, vortexed for 2 min on multi-tube vortexer at SP:SAMPLEPREP_SUMMARY speed 5, centrifuged at 16,000 rcf for 20 min. A 1000 µL aliquot of clean SP:SAMPLEPREP_SUMMARY supernatant was transferred into new pre-labeled LoBind Eppendorf tubes, dried SP:SAMPLEPREP_SUMMARY on the SpeedVac. The dried extracts of samples were reconstituted in 700 µL of SP:SAMPLEPREP_SUMMARY D2O Phosphate buffer NMR Master Mix (containing 0.5 mM DSS-d6 and 0.2% NaN3 SP:SAMPLEPREP_SUMMARY (w/v) in D2O). The sample was vortexed for 2 min on multi-tube vortexer at speed SP:SAMPLEPREP_SUMMARY 5, centrifuged at 12,000 rcf for 4 min., and a 600 µL aliquot of the SP:SAMPLEPREP_SUMMARY supernatant was transferred into numbered 5 mm NMR tubes. Aliquots (350 of µL) SP:SAMPLEPREP_SUMMARY CHEAR Plasma Reference (QC) samples were included in each NMR batch, processed SP:SAMPLEPREP_SUMMARY exactly similar to the study samples described above, and used for QC purposes. SP:SAMPLEPREP_PROTOCOL_FILENAME Dartmouth-ECHO Plasma Metabolomics Procedures #ANALYSIS AN:ANALYSIS_TYPE NMR AN:LABORATORY_NAME Metabolomics and Exposome Lab at UNC Nutrition Research Institute AN:OPERATOR_NAME Wimal Pathmasiri, Kevin Knagge AN:DETECTOR_TYPE NMR AN:SOFTWARE_VERSION TopSpin 3.5 AN:DATA_FORMAT fid, 1r #NMR NM:INSTRUMENT_NAME Avance III 700 MHz NMR Spectrometer NM:INSTRUMENT_TYPE FT-NMR NM:NMR_EXPERIMENT_TYPE 1D-1H NM:FIELD_FREQUENCY_LOCK 3015 Hz, D2O NM:STANDARD_CONCENTRATION 0.5 mM NM:SPECTROMETER_FREQUENCY 700 MHz NM:NMR_PROBE CP QCI/HPCN NM:NMR_SOLVENT 90% H2O, 10% D2O NM:NMR_TUBE_SIZE 5 mm NM:SHIMMING_METHOD topshim NM:PULSE_SEQUENCE noesygppr1d NM:WATER_SUPPRESSION pre-saturation NM:PULSE_WIDTH 15.57 us NM:POWER_LEVEL 12.3 Watts NM:RECEIVER_GAIN 32 NM:OFFSET_FREQUENCY 3289.21 NM:PRESATURATION_POWER_LEVEL 0.0000964 Watts NM:CHEMICAL_SHIFT_REF_CPD DSS-d6 NM:TEMPERATURE 25 C NM:NUMBER_OF_SCANS 128 NM:DUMMY_SCANS 4 NM:ACQUISITION_TIME 3.9 seconds NM:RELAXATION_DELAY 2 seconds NM:SPECTRAL_WIDTH 12 ppm NM:NUM_DATA_POINTS_ACQUIRED 65536 NM:REAL_DATA_POINTS 32768 NM:LINE_BROADENING 0.5 Hz NM:ZERO_FILLING Yes NM:APODIZATION Lorentzian NM:BASELINE_CORRECTION_METHOD Polynomial NM:CHEMICAL_SHIFT_REF_STD DSS-d6 NM:BINNED_INCREMENT 0.04ppm NM:BINNED_DATA_EXCLUDED_RANGE 2.53 - 2.62 ppm (EDTA 01), 2.68 - 2.72 ppm (EDTA 02 ), 3.03 - 3.37 ppm NM:BINNED_DATA_EXCLUDED_RANGE (Methanol), 3.57 - 3.66 ppm (EDTA 03 ), 4.79-4.90 ppm (Water) NM:NMR_RESULTS_FILE wpathmasiri_20210302_074234_NM_Dartmouth_ECHO_OIF_Metabolomics_Normalized_Binned_Data.txt UNITS:ppm #END