#METABOLOMICS WORKBENCH Mette_Hedemann_20210315_030620 DATATRACK_ID:2527 STUDY_ID:ST001724 ANALYSIS_ID:AN002808 PROJECT_ID:000000
VERSION             	1
CREATED_ON             	March 17, 2021, 9:48 am
#PROJECT
PR:PROJECT_TITLE                 	The effects of birth weight and breeding value for protein deposition on the
PR:PROJECT_TITLE                 	urine metabolome in growing pigs
PR:PROJECT_SUMMARY               	An experiment was conducted with growing pigs, to determine the effects of birth
PR:PROJECT_SUMMARY               	weight (BiW) and estimated breeding value for protein deposition (EBV) on the
PR:PROJECT_SUMMARY               	metabolomic profile in urine samples collected under different dietary regimens:
PR:PROJECT_SUMMARY               	protein adequate (A) or protein restricted (R, 70% of A).
PR:INSTITUTE                     	Aarhus University
PR:DEPARTMENT                    	Animal Science
PR:LABORATORY                    	Metabolomics LC-MS platform Aarhus University Foulum
PR:LAST_NAME                     	Hedemann
PR:FIRST_NAME                    	Mette
PR:ADDRESS                       	Blichers Alle 20, Tjele, -, 8830, Denmark
PR:EMAIL                         	Mette.Hedemann@anis.au.dk
PR:PHONE                         	51448783
PR:FUNDING_SOURCE                	European Union's H2020 Program
PR:PROJECT_COMMENTS              	Feed-a-Gene, Grant agreement no 633531
PR:PUBLICATIONS                  	The effects of birth weight and breeding value for protein deposition on
PR:PUBLICATIONS                  	nitrogen efficiency in growing pigs
PR:CONTRIBUTORS                  	C.M.C. van der Peet-Schwering, L.M.G. Verschuren, R. Bergsma, M.S. Hedemann,
PR:CONTRIBUTORS                  	G.P. Binnendijk, A.J.M. Jansman
#STUDY
ST:STUDY_TITLE                   	The effects of birth weight and breeding value for protein deposition on the
ST:STUDY_TITLE                   	urine metabolome in growing pigs (part-III)
ST:STUDY_SUMMARY                 	An experiment was conducted with growing pigs, to determine the effects of birth
ST:STUDY_SUMMARY                 	weight (BiW) and estimated breeding value for protein deposition (EBV) on the
ST:STUDY_SUMMARY                 	metabolomic profile in urine samples collected under different dietary regimens:
ST:STUDY_SUMMARY                 	protein adequate (A) or protein restricted (R, 70% of A).
ST:INSTITUTE                     	Aarhus University
ST:DEPARTMENT                    	Animal Science
ST:LABORATORY                    	Metabolomics LC-MS platform Aarhus University Foulum
ST:LAST_NAME                     	Hedemann
ST:FIRST_NAME                    	Mette
ST:ADDRESS                       	Blichers Alle 20, Tjele, -, 8830, Denmark
ST:EMAIL                         	Mette.Hedemann@anis.au.dk
ST:PHONE                         	51448783
ST:TOTAL_SUBJECTS                	40
ST:NUM_MALES                     	40
ST:PUBLICATIONS                  	The effects of birth weight and breeding value for protein deposition on
ST:PUBLICATIONS                  	nitrogen efficiency in growing pigs
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Sus scrofa
SU:TAXONOMY_ID                   	9823
SU:AGE_OR_AGE_RANGE              	0-125 days
SU:WEIGHT_OR_WEIGHT_RANGE        	1-80 kg
SU:GENDER                        	Male
SU:ANIMAL_ANIMAL_SUPPLIER        	Swine Innovation Centre Sterksel, the Netherlands
SU:ANIMAL_HOUSING                	Metabolism cages
SU:ANIMAL_LIGHT_CYCLE            	12h light / 12h darknes
SU:ANIMAL_FEED                   	Experimental diets (see publication)
SU:ANIMAL_WATER                  	Ad libitum
SU:ANIMAL_INCLUSION_CRITERIA     	Birth weight
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	QC	QC.1_1	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-1_neg_1.mzXML QC-1_pos_1.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC.2_1	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-1_neg_2.mzXML QC-1_pos_2.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC.3_1	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-1_neg_3.mzXML QC-1_pos_3.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC.4_1	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-1_neg_4.mzXML QC-1_pos_4.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9001	1	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=10.325; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_1.mzXML Urin_pos_1.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9189	2	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=1.003; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_2.mzXML Urin_pos_2.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9003	3	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=9.259; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_3.mzXML Urin_pos_3.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9294	4	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=-4.591; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_4.mzXML Urin_pos_4.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9215	5	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=-4.771; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_5.mzXML Urin_pos_5.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9096	6	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=5.035; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_6.mzXML Urin_pos_6.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9104	7	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=7.067; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_7.mzXML Urin_pos_7.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9300	8	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=0.142; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_8.mzXML Urin_pos_8.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9150	9	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=-4.872; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_9.mzXML Urin_pos_9.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9062	10	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=13.209; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_10.mzXML Urin_pos_10.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9219	11	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=0.031; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_11.mzXML Urin_pos_11.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9221	12	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=-3.47; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_12.mzXML Urin_pos_12.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9303	13	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=9.738; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_13.mzXML Urin_pos_13.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9154	14	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=-0.558; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_14.mzXML Urin_pos_14.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9325	15	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=0.882; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_15.mzXML Urin_pos_15.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9188	16	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=8.689; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_16.mzXML Urin_pos_16.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9310	17	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=16.944; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_17.mzXML Urin_pos_17.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9006	18	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=13.239; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_18.mzXML Urin_pos_18.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9149	19	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=-3.794; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_19.mzXML Urin_pos_19.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9004	20	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=18.784; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_20.mzXML Urin_pos_20.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9309	21	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=14.658; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_21.mzXML Urin_pos_21.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9034	22	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=0.057; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_22.mzXML Urin_pos_22.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9171	23	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=-2.49; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_23.mzXML Urin_pos_23.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9125	24	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=10.481; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_24.mzXML Urin_pos_24.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC.1_2	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-2_neg_1.mzXML QC-2_pos_1.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC.2_2	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-2_neg_2.mzXML QC-2_pos_2.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC.3_2	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-2_neg_3.mzXML QC-2_pos_3.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC.4_2	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-2_neg_4.mzXML QC-2_pos_4.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9304	25	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=14.534; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_25.mzXML Urin_pos_25.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9407	26	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=8.842; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_26.mzXML Urin_pos_26.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9315	27	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=0.82; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_27.mzXML Urin_pos_27.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9410	28	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=14.929; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_28.mzXML Urin_pos_28.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9305	29	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=12.996; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_29.mzXML Urin_pos_29.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9247	30	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=-12.409; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_30.mzXML Urin_pos_30.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9152	31	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=-2.114; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_31.mzXML Urin_pos_31.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9296	32	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=0.786; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_32.mzXML Urin_pos_32.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9295	33	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=-3.789; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_33.mzXML Urin_pos_33.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9266	34	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=7.079; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_34.mzXML Urin_pos_34.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9187	35	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=7.204; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_35.mzXML Urin_pos_35.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9031	36	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=8.422; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_36.mzXML Urin_pos_36.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9117	37	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=-2.702; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_37.mzXML Urin_pos_37.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9121	38	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=-1.224; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_38.mzXML Urin_pos_38.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9177	39	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=5.137; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_39.mzXML Urin_pos_39.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9001	40	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=10.325; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_40.mzXML Urin_pos_40.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9189	41	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=1.003; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_41.mzXML Urin_pos_41.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9003	42	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=9.259; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_42.mzXML Urin_pos_42.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9294	43	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=-4.591; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_43.mzXML Urin_pos_43.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9215	44	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=-4.771; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_44.mzXML Urin_pos_44.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9096	45	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=5.035; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_45.mzXML Urin_pos_45.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9104	46	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=7.067; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_46.mzXML Urin_pos_46.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9300	47	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=0.142; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_47.mzXML Urin_pos_47.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9150	48	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=-4.872; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_48.mzXML Urin_pos_48.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC.1_3	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-3_neg_1.mzXML QC-3_pos_1.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC.2_3	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-3_neg_2.mzXML QC-3_pos_2.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC.3_3	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-3_neg_3.mzXML QC-3_pos_3.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC.4_3	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-3_neg_4.mzXML QC-3_pos_4.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9062	49	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=13.209; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_49.mzXML Urin_pos_49.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9219	50	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=0.031; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_50.mzXML Urin_pos_50.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9221	51	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=-3.47; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_51.mzXML Urin_pos_51.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9303	52	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=9.738; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_52.mzXML Urin_pos_52.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9154	53	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=-0.558; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_53.mzXML Urin_pos_53.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9325	54	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=0.882; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_54.mzXML Urin_pos_54.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9188	55	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=8.689; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_55.mzXML Urin_pos_55.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9310	56	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=16.944; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_56.mzXML Urin_pos_56.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9006	57	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=13.239; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_57.mzXML Urin_pos_57.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9149	58	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=-3.794; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_58.mzXML Urin_pos_58.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9004	59	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=18.784; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_59.mzXML Urin_pos_59.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9309	60	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=14.658; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_60.mzXML Urin_pos_60.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9034	61	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=0.057; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_61.mzXML Urin_pos_61.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9171	62	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=-2.49; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_62.mzXML Urin_pos_62.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9125	63	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=10.481; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_63.mzXML Urin_pos_63.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9304	64	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=14.534; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_64.mzXML Urin_pos_64.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9407	65	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=8.842; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_65.mzXML Urin_pos_65.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9315	66	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=0.82; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_66.mzXML Urin_pos_66.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9410	67	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=14.929; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_67.mzXML Urin_pos_67.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9305	68	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=12.996; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_68.mzXML Urin_pos_68.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9247	69	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=-12.409; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_69.mzXML Urin_pos_69.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9152	70	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=-2.114; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_70.mzXML Urin_pos_70.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9296	71	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=0.786; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_71.mzXML Urin_pos_71.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9295	72	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=-3.789; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_72.mzXML Urin_pos_72.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9266	73	Diet:restricted | Wght_birth_cat:high	Breedvalue_animal=7.079; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_73.mzXML Urin_pos_73.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9187	74	Diet:adequate | Wght_birth_cat:high	Breedvalue_animal=7.204; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_74.mzXML Urin_pos_74.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9031	75	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=8.422; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_75.mzXML Urin_pos_75.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9117	76	Diet:adequate | Wght_birth_cat:low	Breedvalue_animal=-2.702; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_76.mzXML Urin_pos_76.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9121	77	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=-1.224; Breedvalue_cat=low; RAW_FILE_NAME=Urin_neg_77.mzXML Urin_pos_77.mzXML
SUBJECT_SAMPLE_FACTORS           	MM9177	78	Diet:restricted | Wght_birth_cat:low	Breedvalue_animal=5.137; Breedvalue_cat=high; RAW_FILE_NAME=Urin_neg_78.mzXML Urin_pos_78.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC.1_4	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-4_neg_1.mzXML QC-4_pos_1.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC.2_4	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-4_neg_2.mzXML QC-4_pos_2.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC.3_4	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-4_neg_3.mzXML QC-4_pos_3.mzXML
SUBJECT_SAMPLE_FACTORS           	QC	QC.4_4	Diet:QC | Wght_birth_cat:QC	Breedvalue_animal=QC; Breedvalue_cat=QC; RAW_FILE_NAME=QC-4_neg_4.mzXML QC-4_pos_4.mzXML
#COLLECTION
CO:COLLECTION_SUMMARY            	In both balance periods, at d4 between 0900 and 1000 h three 1.5 ml urine
CO:COLLECTION_SUMMARY            	samples per pig were taken from the buckets with urine collected from 0800 h
CO:COLLECTION_SUMMARY            	that morning. Before taking the urine samples, the urine was mixed. The samples
CO:COLLECTION_SUMMARY            	were stored at -80°C pending analyses.
CO:SAMPLE_TYPE                   	Urine
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	At an age of 14 weeks, 10 LBW-LEBV (BiW: 1.07 + 0.09 kg; EBV: -2.52 + 3.97 g/d,
TR:TREATMENT_SUMMARY             	compared to an average crossbred pig with a protein deposition of 165 g/d), 10
TR:TREATMENT_SUMMARY             	LBW-HEBV (BiW: 1.02 + 0.13 kg; EBV: 10.47 + 4.26 g/d), 10 HBW-LEBV (BiW: 1.80 +
TR:TREATMENT_SUMMARY             	0.13 kg; EBV: - 2.15 + 2.28 g/d), and 10 HBW-HEBV (BiW: 1.80 + 0.15 kg; EBV:
TR:TREATMENT_SUMMARY             	11.18 + 3.68 g/d), male growing pigs (Synthetic boar x (Dutch Landrace x Large
TR:TREATMENT_SUMMARY             	White)) were allotted to the experiment. The pigs were individually housed in
TR:TREATMENT_SUMMARY             	metabolism cages (1.80 x 0.80 m) at a room temperature of 22oC. They were
TR:TREATMENT_SUMMARY             	subjected to N- balance measurements in two sequential periods of 5 d using a
TR:TREATMENT_SUMMARY             	restricted feeding regime. After a 6-d adaptation period to the metabolism
TR:TREATMENT_SUMMARY             	cages, pigs were adapted for 5 days to the experimental diets before the start
TR:TREATMENT_SUMMARY             	of the first 5-d balance period. Pigs were assigned to a protein adequate (A) or
TR:TREATMENT_SUMMARY             	protein restricted (R, 70% of A) regime in a change-over design. LBW: Low birth
TR:TREATMENT_SUMMARY             	weight; HBW: High birth weight; LEBV: Low estimated breeding value for protein
TR:TREATMENT_SUMMARY             	deposition; HEBV: High estimated breeding value for protein deposition.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	In a 96-well plate, the urine samples (112.5 µL) were diluted with H2O (112.5
SP:SAMPLEPREP_SUMMARY            	µL) and 25 µL acetonitrile (100%, ACN) containing an internal standard mix of
SP:SAMPLEPREP_SUMMARY            	glycocholic acid (glycine-1-13C) and p-chlorophenylalanine to a final
SP:SAMPLEPREP_SUMMARY            	concentration of 0.01 mg/mL was added. The samples were incubated for 20 min at
SP:SAMPLEPREP_SUMMARY            	4°C and subsequently centrifuged (3700 rpm, 25 min, 4°C). The supernatant, 100
SP:SAMPLEPREP_SUMMARY            	µL per well, was transferred to a 200 µL 96-well plate and a protective film
SP:SAMPLEPREP_SUMMARY            	was welded on the plate using a heat sealer, and the plate was centrifuged at
SP:SAMPLEPREP_SUMMARY            	3700 rpm, 4°C for 25 min prior to the LC-MS analysis. Samples were kept in the
SP:SAMPLEPREP_SUMMARY            	autosampler at 10°C, and the injection volume was 3 µl.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Chromatographic separation was performed on a Dionex UltiMate 3000RSL Binary
CH:CHROMATOGRAPHY_SUMMARY        	UHPLC System (Thermo Scientific Dionex, Sunnyvale, CA) equipped with a HSS T3
CH:CHROMATOGRAPHY_SUMMARY        	C18 UHPLC column, 1.8 µm, 100x2.1 mm (Waters Corporation, Milford, MA). The
CH:CHROMATOGRAPHY_SUMMARY        	column was maintained at 30°C. Samples were kept in the autosampler at 10°C,
CH:CHROMATOGRAPHY_SUMMARY        	and the injection volume was 3 µl. The mobile phases were 0.1% formic acid in
CH:CHROMATOGRAPHY_SUMMARY        	Milli-Q water (A) and 0.1% formic acid in acetonitrile (B). The gradient program
CH:CHROMATOGRAPHY_SUMMARY        	for the urine samples was as follows: 0-8 min, linear gradient from 5-70% B;
CH:CHROMATOGRAPHY_SUMMARY        	8-8.5 min, linear gradient from 70-100% B; 8.5-9.5 min, 100% B and return to
CH:CHROMATOGRAPHY_SUMMARY        	initial conditions in 0.2 min. The column was re-equilibrated at 5% B for 2 min
CH:CHROMATOGRAPHY_SUMMARY        	at the beginning of each run and the flow rate was set to 400 µL/min.
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Thermo Dionex Ultimate 3000 RS
CH:COLUMN_NAME                   	Waters Acquity BEH HSS T3 (100 x 2.1mm, 1.8um)
CH:FLOW_RATE                     	0.4 ml/min
CH:COLUMN_TEMPERATURE            	30
CH:SOLVENT_A                     	0.1% formic acid in water
CH:SOLVENT_B                     	0.1% formic acid in acetonitrile
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Bruker Impact HD
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	The eluent was introduced into the mass spectrometer by electrospray ionization,
MS:MS_COMMENTS                   	with capillary set in the positive and negative mode to 4500 and 3600 V,
MS:MS_COMMENTS                   	respectively. End-plate offset voltage was set to 500 V. Nitrogen was used as
MS:MS_COMMENTS                   	both nebulizer and drying gas with a gas pressure of 1.8 bar. Drying gas
MS:MS_COMMENTS                   	temperature and flow were 200 °C and 8.0 L/min, respectively. Spectra were
MS:MS_COMMENTS                   	acquired over the scan range of 50−1000 m/z. A solution of lithium formate
MS:MS_COMMENTS                   	clusters (5 mM) (water/isopropanol/formic acid in a 50:50:0.2 v/v/v ratio) was
MS:MS_COMMENTS                   	injected prior to each chromatographic run as an external calibrant.
MS:MS_RESULTS_FILE               	ST001724_AN002808_Results.txt	UNITS:peak area	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END