#METABOLOMICS WORKBENCH epannkuk_20210511_114559_mwtab.txt DATATRACK_ID:2636 STUDY_ID:ST001791 ANALYSIS_ID:AN002906 PROJECT_ID:000000 VERSION 1 CREATED_ON May 11, 2021, 12:13 pm #PROJECT PR:PROJECT_TITLE LDR_mouse_biofluid_metabolomics PR:PROJECT_SUMMARY An important component of ionizing radiation (IR) exposure after a radiological PR:PROJECT_SUMMARY incident may include low-dose rate (LDR) exposures either externally or PR:PROJECT_SUMMARY internally, such as from 137Cs deposition. LDR exposures can have different PR:PROJECT_SUMMARY effects compared to acute high-dose rate exposures from a health and PR:PROJECT_SUMMARY biodosimetry perspective. In this study, a novel irradiation system, VAriable PR:PROJECT_SUMMARY Dose-rate External 137Cs irradiatoR (VADER), was used to expose male and female PR:PROJECT_SUMMARY mice to a variable LDR over a 30-day time span to cumulative doses of 1 (only in PR:PROJECT_SUMMARY males), 2, 2.8, 4.1, 8.8 (only in males), or 9.7 Gy to simulate fall-out type PR:PROJECT_SUMMARY exposures. Urine and serum from mice exposed to an acute dose (~0.8 Gy/min) of PR:PROJECT_SUMMARY x-rays were collected in parallel. Radiation markers were identified by global PR:PROJECT_SUMMARY mass spectrometry based metabolomics and the machine learning algorithm Random PR:PROJECT_SUMMARY Forests. PR:INSTITUTE Georgetown University PR:LAST_NAME Pannkuk PR:FIRST_NAME Evan PR:ADDRESS 3970 Reservoir Rd, NW New Research Building E504 PR:EMAIL elp44@georgetown.edu PR:PHONE 2026875650 #STUDY ST:STUDY_TITLE Effect of external low-dose rate radiation on mouse biofluid metabolomic ST:STUDY_TITLE signatures ST:STUDY_SUMMARY An important component of ionizing radiation (IR) exposure after a radiological ST:STUDY_SUMMARY incident may include low-dose rate (LDR) exposures either externally or ST:STUDY_SUMMARY internally, such as from 137Cs deposition. LDR exposures can have different ST:STUDY_SUMMARY effects compared to acute high-dose rate exposures from a health and ST:STUDY_SUMMARY biodosimetry perspective. In this study, a novel irradiation system, VAriable ST:STUDY_SUMMARY Dose-rate External 137Cs irradiatoR (VADER), was used to expose male and female ST:STUDY_SUMMARY mice to a variable LDR over a 30-day time span to cumulative doses of 1 (only in ST:STUDY_SUMMARY males), 2, 2.8, 4.1, 8.8 (only in males), or 9.7 Gy to simulate fall-out type ST:STUDY_SUMMARY exposures. Urine and serum from mice exposed to an acute dose (~0.8 Gy/min) of ST:STUDY_SUMMARY x-rays were collected in parallel. Radiation markers were identified by global ST:STUDY_SUMMARY mass spectrometry based metabolomics and the machine learning algorithm Random ST:STUDY_SUMMARY Forests. ST:INSTITUTE Georgetown University ST:LAST_NAME Pannkuk ST:FIRST_NAME Evan ST:ADDRESS 3970 Reservoir Rd, NW New Research Building E504 ST:EMAIL elp44@georgetown.edu ST:PHONE 2026875650 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENOTYPE_STRAIN C57BL/6 SU:GENDER Female SU:ANIMAL_ANIMAL_SUPPLIER Charles River #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS D3C 9 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_009 SUBJECT_SAMPLE_FACTORS D3C 10 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_010 SUBJECT_SAMPLE_FACTORS D5 11 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_011 SUBJECT_SAMPLE_FACTORS D2 12 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_012 SUBJECT_SAMPLE_FACTORS D3 13 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_013 SUBJECT_SAMPLE_FACTORS D5 14 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_014 SUBJECT_SAMPLE_FACTORS D30 15 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_015 SUBJECT_SAMPLE_FACTORS D5C 16 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_016 SUBJECT_SAMPLE_FACTORS D3C 17 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_017 SUBJECT_SAMPLE_FACTORS D3 18 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_018 SUBJECT_SAMPLE_FACTORS D3C 22 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_022 SUBJECT_SAMPLE_FACTORS D3 23 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_023 SUBJECT_SAMPLE_FACTORS D5C 24 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_024 SUBJECT_SAMPLE_FACTORS D3C 25 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_025 SUBJECT_SAMPLE_FACTORS D3 26 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_026 SUBJECT_SAMPLE_FACTORS D2C 27 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_027 SUBJECT_SAMPLE_FACTORS D5C 28 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_028 SUBJECT_SAMPLE_FACTORS D5 29 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_029 SUBJECT_SAMPLE_FACTORS D2 30 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_030 SUBJECT_SAMPLE_FACTORS D2 31 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_031 SUBJECT_SAMPLE_FACTORS D2 35 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_035 SUBJECT_SAMPLE_FACTORS D3C 36 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_036 SUBJECT_SAMPLE_FACTORS D30 37 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_037 SUBJECT_SAMPLE_FACTORS D3C 38 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_038 SUBJECT_SAMPLE_FACTORS D5C 39 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_039 SUBJECT_SAMPLE_FACTORS D3C 40 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_040 SUBJECT_SAMPLE_FACTORS D2 41 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_041 SUBJECT_SAMPLE_FACTORS D5 42 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_042 SUBJECT_SAMPLE_FACTORS D5 43 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_043 SUBJECT_SAMPLE_FACTORS D2C 44 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_044 SUBJECT_SAMPLE_FACTORS D5C 48 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_048 SUBJECT_SAMPLE_FACTORS D3 49 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_049 SUBJECT_SAMPLE_FACTORS D5 50 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_050 SUBJECT_SAMPLE_FACTORS D3 51 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_051 SUBJECT_SAMPLE_FACTORS D3C 52 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_052 SUBJECT_SAMPLE_FACTORS D5 53 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_053 SUBJECT_SAMPLE_FACTORS D5C 54 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_054 SUBJECT_SAMPLE_FACTORS D5C 55 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_055 SUBJECT_SAMPLE_FACTORS D2 56 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_056 SUBJECT_SAMPLE_FACTORS D2C 57 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_057 SUBJECT_SAMPLE_FACTORS D5C 61 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_061 SUBJECT_SAMPLE_FACTORS D3C 62 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_062 SUBJECT_SAMPLE_FACTORS D30C 63 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_063 SUBJECT_SAMPLE_FACTORS D2 64 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_064 SUBJECT_SAMPLE_FACTORS D3C 65 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_065 SUBJECT_SAMPLE_FACTORS D3C 66 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_066 SUBJECT_SAMPLE_FACTORS D2 67 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_067 SUBJECT_SAMPLE_FACTORS D2C 68 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_068 SUBJECT_SAMPLE_FACTORS D2 69 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_069 SUBJECT_SAMPLE_FACTORS D2C 70 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_070 SUBJECT_SAMPLE_FACTORS D30C 74 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_074 SUBJECT_SAMPLE_FACTORS D3 75 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_075 SUBJECT_SAMPLE_FACTORS D2 76 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_076 SUBJECT_SAMPLE_FACTORS D5 77 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_077 SUBJECT_SAMPLE_FACTORS D5C 78 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_078 SUBJECT_SAMPLE_FACTORS D5 79 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_079 SUBJECT_SAMPLE_FACTORS D2C 80 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_080 SUBJECT_SAMPLE_FACTORS D2 81 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_081 SUBJECT_SAMPLE_FACTORS D30 82 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_082 SUBJECT_SAMPLE_FACTORS D30C 86 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_086 SUBJECT_SAMPLE_FACTORS D5 87 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_087 SUBJECT_SAMPLE_FACTORS D2C 88 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_088 SUBJECT_SAMPLE_FACTORS D2 89 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_089 SUBJECT_SAMPLE_FACTORS D3 90 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_090 SUBJECT_SAMPLE_FACTORS D2C 91 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_091 SUBJECT_SAMPLE_FACTORS D30 92 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_092 SUBJECT_SAMPLE_FACTORS D2 93 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_093 SUBJECT_SAMPLE_FACTORS D3C 94 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_094 SUBJECT_SAMPLE_FACTORS D3C 95 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_095 SUBJECT_SAMPLE_FACTORS D3 99 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_099 SUBJECT_SAMPLE_FACTORS D5C 100 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_100 SUBJECT_SAMPLE_FACTORS D5C 101 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_101 SUBJECT_SAMPLE_FACTORS D30 102 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_102 SUBJECT_SAMPLE_FACTORS D30C 103 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_103 SUBJECT_SAMPLE_FACTORS D3C 104 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_104 SUBJECT_SAMPLE_FACTORS D3C 105 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_105 SUBJECT_SAMPLE_FACTORS D5 106 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_106 SUBJECT_SAMPLE_FACTORS D5C 107 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_107 SUBJECT_SAMPLE_FACTORS D5 108 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_108 SUBJECT_SAMPLE_FACTORS D3C 112 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_112 SUBJECT_SAMPLE_FACTORS D3C 113 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_113 SUBJECT_SAMPLE_FACTORS D5 114 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_114 SUBJECT_SAMPLE_FACTORS D2 115 Factor:A | Factor:post RAW_FILE_NAME=urine_POS_115 SUBJECT_SAMPLE_FACTORS D30C 116 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_116 SUBJECT_SAMPLE_FACTORS D5 117 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_117 SUBJECT_SAMPLE_FACTORS D3 118 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_118 SUBJECT_SAMPLE_FACTORS D5 119 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_119 SUBJECT_SAMPLE_FACTORS D2C 120 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_120 SUBJECT_SAMPLE_FACTORS D2 121 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_121 SUBJECT_SAMPLE_FACTORS D2C 125 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_125 SUBJECT_SAMPLE_FACTORS D3C 126 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_126 SUBJECT_SAMPLE_FACTORS D2 127 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_127 SUBJECT_SAMPLE_FACTORS D3 128 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_128 SUBJECT_SAMPLE_FACTORS D5 129 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_129 SUBJECT_SAMPLE_FACTORS D5C 130 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_130 SUBJECT_SAMPLE_FACTORS D2 131 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_131 SUBJECT_SAMPLE_FACTORS D2 132 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_132 SUBJECT_SAMPLE_FACTORS D2 133 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_133 SUBJECT_SAMPLE_FACTORS D5C 137 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_137 SUBJECT_SAMPLE_FACTORS D3C 138 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_138 SUBJECT_SAMPLE_FACTORS D5C 139 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_139 SUBJECT_SAMPLE_FACTORS D5C 140 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_140 SUBJECT_SAMPLE_FACTORS D2C 141 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_141 SUBJECT_SAMPLE_FACTORS D30 142 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_142 SUBJECT_SAMPLE_FACTORS D5C 143 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_143 SUBJECT_SAMPLE_FACTORS D5C 144 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_144 SUBJECT_SAMPLE_FACTORS D2C 145 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_145 SUBJECT_SAMPLE_FACTORS D2C 146 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_146 SUBJECT_SAMPLE_FACTORS D2C 150 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_150 SUBJECT_SAMPLE_FACTORS D2C 151 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_151 SUBJECT_SAMPLE_FACTORS D3 152 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_152 SUBJECT_SAMPLE_FACTORS D3 153 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_153 SUBJECT_SAMPLE_FACTORS D3 154 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_154 SUBJECT_SAMPLE_FACTORS D3 155 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_155 SUBJECT_SAMPLE_FACTORS D5 156 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_156 SUBJECT_SAMPLE_FACTORS D3 157 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_157 SUBJECT_SAMPLE_FACTORS D5 158 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_158 SUBJECT_SAMPLE_FACTORS D30C 159 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_159 SUBJECT_SAMPLE_FACTORS D30 163 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_163 SUBJECT_SAMPLE_FACTORS D2C 164 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_164 SUBJECT_SAMPLE_FACTORS D30C 165 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_165 SUBJECT_SAMPLE_FACTORS D3 166 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_166 SUBJECT_SAMPLE_FACTORS D5 167 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_167 SUBJECT_SAMPLE_FACTORS D2C 168 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_168 SUBJECT_SAMPLE_FACTORS D30C 169 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_169 SUBJECT_SAMPLE_FACTORS D3C 170 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_170 SUBJECT_SAMPLE_FACTORS D30 171 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_171 SUBJECT_SAMPLE_FACTORS D3C 172 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_172 SUBJECT_SAMPLE_FACTORS D3 176 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_176 SUBJECT_SAMPLE_FACTORS D2C 177 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_177 SUBJECT_SAMPLE_FACTORS D30 178 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_178 SUBJECT_SAMPLE_FACTORS D2 179 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_179 SUBJECT_SAMPLE_FACTORS D2 180 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_180 SUBJECT_SAMPLE_FACTORS D3 181 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_181 SUBJECT_SAMPLE_FACTORS D30C 182 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_182 SUBJECT_SAMPLE_FACTORS D5 183 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_183 SUBJECT_SAMPLE_FACTORS D3C 184 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_184 SUBJECT_SAMPLE_FACTORS D2C 185 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_185 SUBJECT_SAMPLE_FACTORS D3 189 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_189 SUBJECT_SAMPLE_FACTORS D3 190 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_190 SUBJECT_SAMPLE_FACTORS D2 191 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_191 SUBJECT_SAMPLE_FACTORS D3C 192 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_192 SUBJECT_SAMPLE_FACTORS D5C 193 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_193 SUBJECT_SAMPLE_FACTORS D3 194 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_194 SUBJECT_SAMPLE_FACTORS D5 195 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_195 SUBJECT_SAMPLE_FACTORS D2C 196 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_196 SUBJECT_SAMPLE_FACTORS D5 197 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_197 SUBJECT_SAMPLE_FACTORS D30C 198 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_198 SUBJECT_SAMPLE_FACTORS D2C 202 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_202 SUBJECT_SAMPLE_FACTORS D30 203 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_203 SUBJECT_SAMPLE_FACTORS D30 204 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_204 SUBJECT_SAMPLE_FACTORS D5 205 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_205 SUBJECT_SAMPLE_FACTORS D3 206 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_206 SUBJECT_SAMPLE_FACTORS D2C 207 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_207 SUBJECT_SAMPLE_FACTORS D30 208 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_208 SUBJECT_SAMPLE_FACTORS D2C 209 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_209 SUBJECT_SAMPLE_FACTORS D2 210 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_210 SUBJECT_SAMPLE_FACTORS D3 211 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_211 SUBJECT_SAMPLE_FACTORS D3 215 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_215 SUBJECT_SAMPLE_FACTORS D5C 216 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_216 SUBJECT_SAMPLE_FACTORS D3 217 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_217 SUBJECT_SAMPLE_FACTORS D3 218 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_218 SUBJECT_SAMPLE_FACTORS D3C 219 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_219 SUBJECT_SAMPLE_FACTORS D5 220 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_220 SUBJECT_SAMPLE_FACTORS D2 221 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_221 SUBJECT_SAMPLE_FACTORS D3C 222 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_222 SUBJECT_SAMPLE_FACTORS D5 223 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_223 SUBJECT_SAMPLE_FACTORS D3 224 Factor:HDR | Factor:pre RAW_FILE_NAME=urine_POS_224 SUBJECT_SAMPLE_FACTORS D5C 228 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_228 SUBJECT_SAMPLE_FACTORS D3C 229 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_229 SUBJECT_SAMPLE_FACTORS D2 230 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_230 SUBJECT_SAMPLE_FACTORS D5C 231 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_231 SUBJECT_SAMPLE_FACTORS D2 232 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_232 SUBJECT_SAMPLE_FACTORS D30 233 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_233 SUBJECT_SAMPLE_FACTORS D3C 234 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_234 SUBJECT_SAMPLE_FACTORS D3 235 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_235 SUBJECT_SAMPLE_FACTORS D5 236 Factor:HDR | Factor:post RAW_FILE_NAME=urine_POS_236 SUBJECT_SAMPLE_FACTORS D30C 237 Factor:Control | Factor:pre RAW_FILE_NAME=urine_POS_237 SUBJECT_SAMPLE_FACTORS D5C 238 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_238 SUBJECT_SAMPLE_FACTORS D2 239 Factor:LDR | Factor:pre RAW_FILE_NAME=urine_POS_239 SUBJECT_SAMPLE_FACTORS D5 240 Factor:LDR | Factor:post RAW_FILE_NAME=urine_POS_240 SUBJECT_SAMPLE_FACTORS D3C 241 Factor:Control | Factor:post RAW_FILE_NAME=urine_POS_241 #COLLECTION CO:COLLECTION_SUMMARY Spot urine collected before and after irradiation CO:SAMPLE_TYPE Urine CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY The VADER was designed to deliver controlled dose rates in the range 0.1 – 1 TR:TREATMENT_SUMMARY Gy/day to a cohort of up to 15 mice. The VADER uses ~0.5 Ci of retired 137Cs TR:TREATMENT_SUMMARY brachytherapy seeds that are arranged in two platters placed above and below a TR:TREATMENT_SUMMARY “mouse hotel”. The platters can be placed ~0.5 – 60 cm above and below the TR:TREATMENT_SUMMARY mouse hotel allowing implementation of time-variable dose rates. Offline TR:TREATMENT_SUMMARY dosimetry of the VADER was performed annually using a NIST traceable 10x6-6 TR:TREATMENT_SUMMARY ionization chamber (Radcal Corp., Monrovia, CA). Dose uniformity across the TR:TREATMENT_SUMMARY surface was measured using EBT3 film (Ashland, Covington, KY, USA) and the TR:TREATMENT_SUMMARY variation was 15% across the hotel. A lead and high-density concrete brick TR:TREATMENT_SUMMARY shield ensured minimal radiation doses to occupationally exposed personnel TR:TREATMENT_SUMMARY (operators) inside (< 0.1 mGy/wk) and outside the room (< 0.02 mGy/wk). The TR:TREATMENT_SUMMARY mouse hotel consists of an acrylic box (35 x 35 x 12 cm) allowing housing of ≤ TR:TREATMENT_SUMMARY 15 mice with bedding material and food/water ad libitum. Temperature (20 – TR:TREATMENT_SUMMARY 25°C), humidity (40 – 60%), airflow and lighting were fully controlled to TR:TREATMENT_SUMMARY required animal care standards (temperature/humidity sensor, HWg HTemp, TruePath TR:TREATMENT_SUMMARY Technologies Victor, NY). Environmental controls and monitoring were integrated TR:TREATMENT_SUMMARY into the mouse hotel for easy replacement in case of radiation damage. Mice were TR:TREATMENT_SUMMARY monitored in real time using a 180° fisheye ELP USB camera (Amazon). All animal TR:TREATMENT_SUMMARY experiments were approved by the Columbia University Institutional Animal Care TR:TREATMENT_SUMMARY and Use Committee (IACUC; approved protocol AAQ2410) and were conducted under TR:TREATMENT_SUMMARY all relevant federal and state guidelines. Male and female C57BL/6 mice ( TR:TREATMENT_SUMMARY Charles River Laboratories, Frederick, MD, USA) were irradiated in the VADER in TR:TREATMENT_SUMMARY two randomized batches (15 mice) loaded into mouse hotels (one hotel loaded into TR:TREATMENT_SUMMARY the VADER and one in the same room as a zero-dose control). Five mice per time TR:TREATMENT_SUMMARY point were irradiated in two back-to-back runs (run 1: 2, 3, or 5 d; run 2: 5, TR:TREATMENT_SUMMARY 20, or 30 d) to a total dose of 1 Gy (1 d), 2 Gy (2 d), 2.8 Gy (3 d), 4.1 Gy (5 TR:TREATMENT_SUMMARY d), 8.8 Gy (20 d), and 9.7 Gy (30 d) (Figure S1). Urine and serum were flash TR:TREATMENT_SUMMARY frozen and then stored at -80°C until shipped to Georgetown University Medical TR:TREATMENT_SUMMARY Center for analysis. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Samples were prepared and analyzed as previously described.18, 19 Briefly, serum SP:SAMPLEPREP_SUMMARY (5 μl) was deproteinized (195 μl 66% cold acetonitrile [ACN]) with internal SP:SAMPLEPREP_SUMMARY standards (2 μM debrisoquine [M+H]+ = 176.1188; 30 μM 4-nitrobenzoic acid SP:SAMPLEPREP_SUMMARY [M-H]- = 166.0141), vortexed, incubated on ice (10 min), and centrifuged for 10 SP:SAMPLEPREP_SUMMARY min (max speed, 4 °C). Urine (20 μl) was deproteinized (80 μl 50% cold ACN) SP:SAMPLEPREP_SUMMARY and prepared as above. For urine and serum 1 μl of each sample were combined SP:SAMPLEPREP_SUMMARY for a quality control (QC) sample. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Mobile phases consisted of the following: solvent A (water/0.1% formic acid CH:CHROMATOGRAPHY_SUMMARY [FA]), solvent B (ACN/0.1% FA), solvent C (isopropanol [IPA]/ACN (90:10)/0.1% CH:CHROMATOGRAPHY_SUMMARY FA). The gradient for urine was (solvent A and B) 4.0 min 5% B, 4.0 min 20% B, CH:CHROMATOGRAPHY_SUMMARY 5.1 min 95% B, and 1.9 min 5% B at a flow rate of 0.5 ml/min. The gradient for CH:CHROMATOGRAPHY_SUMMARY serum was (solvent A, B, and C) 4.0 min 98:2 A:B, 4.0 min 40:60 A:B, 1.5 min CH:CHROMATOGRAPHY_SUMMARY 2:98 A:B, 2.0 min 2:98 A:C, 0.5 min 50:50 A:C, and 1.0 min 98:2 A:B at a flow CH:CHROMATOGRAPHY_SUMMARY rate of 0.5 ml/min. CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Waters Acquity CH:COLUMN_NAME Waters Acquity BEH C18 (50 x 2.1mm, 1.7 um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Waters Synapt G2 S QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS Operating conditions for ESI were: capillary voltage 2.75 kV, cone voltage 30 V, MS:MS_COMMENTS desolvation temperature 500°C, desolvation gas flow 1000 L/Hr. MS:MS_RESULTS_FILE ST001791_AN002906_Results.txt UNITS:peak area Has m/z:Yes Has RT:Yes RT units:Minutes #END