#METABOLOMICS WORKBENCH tnuriel_20210602_140903_mwtab.txt DATATRACK_ID:2671 STUDY_ID:ST001819 ANALYSIS_ID:AN002952 PROJECT_ID:PR001150
VERSION             	1
CREATED_ON             	June 7, 2021, 12:24 pm
#PROJECT
PR:PROJECT_TYPE                  	Lipidomics analysis
PR:PROJECT_SUMMARY               	Apolipoprotein E ε4 (APOE4) is the primary genetic risk factor for the
PR:PROJECT_SUMMARY               	late-onset form of Alzheimer's disease (AD). Although the reason for this
PR:PROJECT_SUMMARY               	association is not completely understood, researchers have uncovered numerous
PR:PROJECT_SUMMARY               	effects of APOE4 expression on AD-relevant brain processes, including amyloid
PR:PROJECT_SUMMARY               	beta (Aβ) accumulation, lipid metabolism, endosomal-lysosomal processing and
PR:PROJECT_SUMMARY               	bioenergetics. In this study, we aimed to determine the effect of APOE4 allelic
PR:PROJECT_SUMMARY               	dosage on regional brain lipid composition in aged mice, as well as in cultured
PR:PROJECT_SUMMARY               	neurons. We performed a targeted lipidomic analysis on the entorhinal cortex
PR:PROJECT_SUMMARY               	(EC) and primary visual cortex (PVC) from 14–15 month-old APOE3/3, APOE3/4,
PR:PROJECT_SUMMARY               	and APOE4/4 targeted replacement mice, as well as on WT neurons cultured with
PR:PROJECT_SUMMARY               	conditioned media from APOE3/3 or APOE4/4 astrocytes. Our results reveal that
PR:PROJECT_SUMMARY               	the EC possesses increased susceptibility to APOE4-associated lipid alterations
PR:PROJECT_SUMMARY               	compared to the PVC. In the EC, APOE4 expression showed a dominant effect in
PR:PROJECT_SUMMARY               	decreasing diacylglycerol (DAG) levels, and a semi-dominant additive effect in
PR:PROJECT_SUMMARY               	the upregulation of multiple ceramide, glycosylated sphingolipid and
PR:PROJECT_SUMMARY               	bis(monoacylglycerol)phosphate (BMP) species, lipids known to accumulate as a
PR:PROJECT_SUMMARY               	result of endosomal-lysosomal dysfunction and defective lysosomal clearance.
PR:PROJECT_SUMMARY               	Neurons treated with conditioned media from APOE4 vs. APOE3 astrocytes also
PR:PROJECT_SUMMARY               	showed similar alterations of DAG and BMP species as those observed in the mouse
PR:PROJECT_SUMMARY               	EC. Our results suggest that APOE4 expression differentially modulates regional
PR:PROJECT_SUMMARY               	and neuronal lipid signatures, which may underlie the increased susceptibility
PR:PROJECT_SUMMARY               	of EC-localized neurons to AD pathology.
PR:INSTITUTE                     	Columbia University
PR:FIRST_NAME                    	Tal
PR:ADDRESS                       	630 W 168th St., P&S 12-430
PR:EMAIL                         	tn2283@cumc.columbia.edu
PR:PHONE                         	2123045683
PR:PROJECT_TITLE                 	APOE4-associated differences in lipidomics signatures in mouse brain and
PR:PROJECT_TITLE                 	cultured neurons
PR:LAST_NAME                     	Nuriel
#STUDY
ST:STUDY_TITLE                   	EC and PVC from 14-15 month-old APOE3/3, APOE3/4 and APOE4/4 mice
ST:STUDY_SUMMARY                 	We performed a targeted lipidomic analysis on EC and PVC tissue from 14-15 month
ST:STUDY_SUMMARY                 	old APOE3/3, APOE3/4, and APOE4/4 mice.
ST:INSTITUTE                     	Columbia University
ST:LAST_NAME                     	Nuriel
ST:FIRST_NAME                    	Tal
ST:ADDRESS                       	630 W 168th St., P&S 12-430
ST:EMAIL                         	tn2283@cumc.columbia.edu
ST:PHONE                         	2123045683
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:AGE_OR_AGE_RANGE              	14-15 month-old
SU:GENDER                        	Male
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	EC1	Region:EC | Genotype:E3/3	
SUBJECT_SAMPLE_FACTORS           	-	EC2	Region:EC | Genotype:E3/3	
SUBJECT_SAMPLE_FACTORS           	-	EC3	Region:EC | Genotype:E3/3	
SUBJECT_SAMPLE_FACTORS           	-	EC4	Region:EC | Genotype:E3/3	
SUBJECT_SAMPLE_FACTORS           	-	EC5	Region:EC | Genotype:E3/3	
SUBJECT_SAMPLE_FACTORS           	-	EC6	Region:EC | Genotype:E3/3	
SUBJECT_SAMPLE_FACTORS           	-	EC7	Region:EC | Genotype:E3/3	
SUBJECT_SAMPLE_FACTORS           	-	EC8	Region:EC | Genotype:E3/3	
SUBJECT_SAMPLE_FACTORS           	-	EC10	Region:EC | Genotype:E3/4	
SUBJECT_SAMPLE_FACTORS           	-	EC12	Region:EC | Genotype:E3/4	
SUBJECT_SAMPLE_FACTORS           	-	EC13	Region:EC | Genotype:E3/4	
SUBJECT_SAMPLE_FACTORS           	-	EC14	Region:EC | Genotype:E3/4	
SUBJECT_SAMPLE_FACTORS           	-	EC15	Region:EC | Genotype:E3/4	
SUBJECT_SAMPLE_FACTORS           	-	EC16	Region:EC | Genotype:E3/4	
SUBJECT_SAMPLE_FACTORS           	-	EC17	Region:EC | Genotype:E4/4	
SUBJECT_SAMPLE_FACTORS           	-	EC18	Region:EC | Genotype:E4/4	
SUBJECT_SAMPLE_FACTORS           	-	EC19	Region:EC | Genotype:E4/4	
SUBJECT_SAMPLE_FACTORS           	-	EC20	Region:EC | Genotype:E4/4	
SUBJECT_SAMPLE_FACTORS           	-	EC21	Region:EC | Genotype:E4/4	
SUBJECT_SAMPLE_FACTORS           	-	EC22	Region:EC | Genotype:E4/4	
SUBJECT_SAMPLE_FACTORS           	-	EC23	Region:EC | Genotype:E4/4	
SUBJECT_SAMPLE_FACTORS           	-	EC24	Region:EC | Genotype:E4/4	
SUBJECT_SAMPLE_FACTORS           	-	PVC1	Region:PVC | Genotype:E3/3	
SUBJECT_SAMPLE_FACTORS           	-	PVC2	Region:PVC | Genotype:E3/3	
SUBJECT_SAMPLE_FACTORS           	-	PVC3	Region:PVC | Genotype:E3/3	
SUBJECT_SAMPLE_FACTORS           	-	PVC4	Region:PVC | Genotype:E3/3	
SUBJECT_SAMPLE_FACTORS           	-	PVC5	Region:PVC | Genotype:E3/3	
SUBJECT_SAMPLE_FACTORS           	-	PVC6	Region:PVC | Genotype:E3/3	
SUBJECT_SAMPLE_FACTORS           	-	PVC7	Region:PVC | Genotype:E3/3	
SUBJECT_SAMPLE_FACTORS           	-	PVC9	Region:PVC | Genotype:E3/4	
SUBJECT_SAMPLE_FACTORS           	-	PVC10	Region:PVC | Genotype:E3/4	
SUBJECT_SAMPLE_FACTORS           	-	PVC11	Region:PVC | Genotype:E3/4	
SUBJECT_SAMPLE_FACTORS           	-	PVC12	Region:PVC | Genotype:E3/4	
SUBJECT_SAMPLE_FACTORS           	-	PVC13	Region:PVC | Genotype:E3/4	
SUBJECT_SAMPLE_FACTORS           	-	PVC14	Region:PVC | Genotype:E3/4	
SUBJECT_SAMPLE_FACTORS           	-	PVC15	Region:PVC | Genotype:E3/4	
SUBJECT_SAMPLE_FACTORS           	-	PVC16	Region:PVC | Genotype:E3/4	
SUBJECT_SAMPLE_FACTORS           	-	PVC17	Region:PVC | Genotype:E4/4	
SUBJECT_SAMPLE_FACTORS           	-	PVC19	Region:PVC | Genotype:E4/4	
SUBJECT_SAMPLE_FACTORS           	-	PVC20	Region:PVC | Genotype:E4/4	
SUBJECT_SAMPLE_FACTORS           	-	PVC21	Region:PVC | Genotype:E4/4	
SUBJECT_SAMPLE_FACTORS           	-	PVC22	Region:PVC | Genotype:E4/4	
SUBJECT_SAMPLE_FACTORS           	-	PVC23	Region:PVC | Genotype:E4/4	
SUBJECT_SAMPLE_FACTORS           	-	PVC24	Region:PVC | Genotype:E4/4	
#COLLECTION
CO:COLLECTION_SUMMARY            	Mice were sacrificed by cervical dislocation to maintain the brain environment,
CO:COLLECTION_SUMMARY            	and individual brain regions were immediately removed and snap-frozen on dry
CO:COLLECTION_SUMMARY            	ice. Tissues were stored at -80°C for prior to extraction.
CO:SAMPLE_TYPE                   	Brain
#TREATMENT
TR:TREATMENT_SUMMARY             	No treatments were made. Mice were either APOE3/3, APOE3/4 or APOE4/4 gentoype.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Lipid and small-molecule metabolite extraction was performed using a methyl
SP:SAMPLEPREP_SUMMARY            	tert-butyl ether (MTBE)/methanol extraction protocol modified from previous
SP:SAMPLEPREP_SUMMARY            	reports (40, 41), as we have described previously (34). Briefly, individual EC
SP:SAMPLEPREP_SUMMARY            	or PVC tissues were homogenized in 400 ul of ice-cold methanol using a bead mill
SP:SAMPLEPREP_SUMMARY            	homogenizer (TissueLyser II, Qiagen) at 25 beats/sec, 2x for 45 sec each.
SP:SAMPLEPREP_SUMMARY            	Following homogenization, samples were incubated in 1200 ul of MTBE for 1 hr at
SP:SAMPLEPREP_SUMMARY            	room temperature to separate organic-soluble lipids from aqueous-soluble lipids
SP:SAMPLEPREP_SUMMARY            	and other small-molecules. Finally, 360 ul of ultrapure water was added (for a
SP:SAMPLEPREP_SUMMARY            	final ratio of 3:1:0.9 MTBE:methanol:water) to resolve the two liquid phases,
SP:SAMPLEPREP_SUMMARY            	and each samples were centrifuged at 10,000 x g for 10 min. For this experiment,
SP:SAMPLEPREP_SUMMARY            	the upper organic phase was collected from each sample and stored in a separate
SP:SAMPLEPREP_SUMMARY            	tube, and the remaining protein pellets were resuspended in 25 mM ammonium
SP:SAMPLEPREP_SUMMARY            	bicarbonate, pH 8, with 2.5% SDS. A BCA protein assay was performed on each
SP:SAMPLEPREP_SUMMARY            	protein fraction, and the organic phase was normalized to their protein
SP:SAMPLEPREP_SUMMARY            	concentration equivalent with 100% methanol. All samples were then stored at
SP:SAMPLEPREP_SUMMARY            	-80°C prior to analysis.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Reverse phase negative mode
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1260
CH:COLUMN_NAME                   	Agilent Eclipse XDB-C18 (100 x 3.0mm)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent 6490 QQQ
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	Lipid profiling was performed using an Agilent 1260 HPLC coupled to an Agilent
MS:MS_COMMENTS                   	6490 triple quadrupole (QQQ) mass spectrometer (41). Each sample was run through
MS:MS_COMMENTS                   	three separate chromatographic conditions (reverse-phase negative mode,
MS:MS_COMMENTS                   	reverse-phase positive mode and normal-phase positive mode) for the effective
MS:MS_COMMENTS                   	quantification of 337 distinct lipids from 28 lipid subclasses, as previously
MS:MS_COMMENTS                   	described (41). Individual lipid species were measured by multiple reaction
MS:MS_COMMENTS                   	monitoring transitions.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	area under the curve
MS_METABOLITE_DATA_START
Samples	EC1	EC2	EC3	EC4	EC5	EC6	EC7	EC8	EC10	EC12	EC13	EC14	EC15	EC16	EC17	EC18	EC19	EC20	EC21	EC22	EC23	EC24	PVC1	PVC2	PVC3	PVC4	PVC5	PVC6	PVC7	PVC9	PVC10	PVC11	PVC12	PVC13	PVC14	PVC15	PVC16	PVC17	PVC19	PVC20	PVC21	PVC22	PVC23	PVC24
Factors	Region:EC | Genotype:E3/3	Region:EC | Genotype:E3/3	Region:EC | Genotype:E3/3	Region:EC | Genotype:E3/3	Region:EC | Genotype:E3/3	Region:EC | Genotype:E3/3	Region:EC | Genotype:E3/3	Region:EC | Genotype:E3/3	Region:EC | Genotype:E3/4	Region:EC | Genotype:E3/4	Region:EC | Genotype:E3/4	Region:EC | Genotype:E3/4	Region:EC | Genotype:E3/4	Region:EC | Genotype:E3/4	Region:EC | Genotype:E4/4	Region:EC | Genotype:E4/4	Region:EC | Genotype:E4/4	Region:EC | Genotype:E4/4	Region:EC | Genotype:E4/4	Region:EC | Genotype:E4/4	Region:EC | Genotype:E4/4	Region:EC | Genotype:E4/4	Region:PVC | Genotype:E3/3	Region:PVC | Genotype:E3/3	Region:PVC | Genotype:E3/3	Region:PVC | Genotype:E3/3	Region:PVC | Genotype:E3/3	Region:PVC | Genotype:E3/3	Region:PVC | Genotype:E3/3	Region:PVC | Genotype:E3/4	Region:PVC | Genotype:E3/4	Region:PVC | Genotype:E3/4	Region:PVC | Genotype:E3/4	Region:PVC | Genotype:E3/4	Region:PVC | Genotype:E3/4	Region:PVC | Genotype:E3/4	Region:PVC | Genotype:E3/4	Region:PVC | Genotype:E4/4	Region:PVC | Genotype:E4/4	Region:PVC | Genotype:E4/4	Region:PVC | Genotype:E4/4	Region:PVC | Genotype:E4/4	Region:PVC | Genotype:E4/4	Region:PVC | Genotype:E4/4
Cer 16:0	0.0062	0.0068	0.0068	0.0062	0.0077	0.0068	0.0111	0.0072	0.0074	0.0063	0.0075	0.0074	0.0081	0.0062	0.0113	0.0097	0.0087	0.0089	0.0097	0.0105	0.0096	0.0094	0.0072	0.0085	0.0076	0.0100	0.0063	0.0104	0.0077	0.0069	0.0076	0.0077	0.0066	0.0071	0.0087	0.0087	0.0069	0.0053	0.0060	0.0060	0.0070	0.0062	0.0076	0.0064
Cer 16:1	0.1319	0.1296	0.1371	0.1390	0.1322	0.1265	0.1320	0.1441	0.1288	0.1339	0.1204	0.1272	0.1356	0.1402	0.1302	0.1407	0.1526	0.1664	0.1386	0.1480	0.1419	0.1437	0.1379	0.1305	0.1296	0.1366	0.1324	0.1251	0.1372	0.1345	0.1367	0.1267	0.1226	0.1267	0.1363	0.1335	0.1407	0.1274	0.1413	0.1355	0.1338	0.1417	0.1402	0.1142
Cer 18:0	1.3206	1.5458	1.6941	1.3522	1.6441	1.7111	1.5990	1.4477	1.5546	1.5875	1.5320	1.9963	2.0362	1.4424	1.3407	1.7313	1.4406	1.5769	1.6094	1.7708	1.7988	1.6655	1.0496	1.5946	1.0572	1.4685	1.0354	1.2974	1.2071	0.9461	1.0639	1.1496	1.1718	1.1751	1.5064	1.5699	1.0063	0.6771	1.2450	1.1101	1.2544	1.0171	1.6276	1.0697
Cer 18:1	0.0029	0.0032	0.0027	0.0035	0.0037	0.0050	0.0034	0.0032	0.0042	0.0042	0.0045	0.0050	0.0041	0.0037	0.0083	0.0067	0.0054	0.0055	0.0055	0.0057	0.0070	0.0059	0.0061	0.0062	0.0069	0.0050	0.0042	0.0048	0.0051	0.0060	0.0064	0.0036	0.0049	0.0038	0.0074	0.0062	0.0055	0.0026	0.0051	0.0044	0.0041	0.0052	0.0051	0.0037
Cer 20:0	0.0398	0.0474	0.0476	0.0414	0.0477	0.0527	0.0489	0.0451	0.0462	0.0502	0.0453	0.0600	0.0618	0.0458	0.0407	0.0556	0.0456	0.0471	0.0498	0.0504	0.0487	0.0526	0.0414	0.0474	0.0378	0.0463	0.0332	0.0385	0.0426	0.0340	0.0391	0.0315	0.0380	0.0368	0.0459	0.0495	0.0249	0.0236	0.0387	0.0381	0.0357	0.0549	0.0561	0.0325
Cer 20:1	0.0075	0.0081	0.0071	0.0098	0.0088	0.0099	0.0085	0.0095	0.0106	0.0082	0.0066	0.0139	0.0088	0.0078	0.0170	0.0141	0.0102	0.0080	0.0102	0.0106	0.0092	0.0120	0.0272	0.0158	0.0284	0.0207	0.0205	0.0131	0.0196	0.0233	0.0206	0.0143	0.0157	0.0148	0.0223	0.0162	0.0218	0.0096	0.0222	0.0199	0.0107	0.0287	0.0156	0.0070
Cer 22:0	0.0114	0.0129	0.0125	0.0127	0.0163	0.0191	0.0180	0.0152	0.0137	0.0153	0.0146	0.0202	0.0193	0.0150	0.0181	0.0235	0.0156	0.0150	0.0140	0.0177	0.0154	0.0178	0.0218	0.0134	0.0169	0.0164	0.0125	0.0157	0.0173	0.0183	0.0191	0.0129	0.0160	0.0144	0.0196	0.0162	0.0151	0.0093	0.0170	0.0148	0.0124	0.0262	0.0183	0.0115
Cer 22:1	0.0245	0.0217	0.0152	0.0294	0.0206	0.0254	0.0219	0.0238	0.0294	0.0237	0.0127	0.0478	0.0215	0.0210	0.0608	0.0743	0.0270	0.0215	0.0281	0.0286	0.0264	0.0418	0.1114	0.0484	0.1047	0.0642	0.0766	0.0489	0.0720	0.0816	0.1282	0.0548	0.0606	0.0563	0.0903	0.0568	0.0799	0.0383	0.0755	0.0825	0.0336	0.1470	0.0543	0.0163
Cer 24:0	0.0063	0.0060	0.0070	0.0066	0.0080	0.0117	0.0140	0.0091	0.0068	0.0083	0.0076	0.0094	0.0110	0.0079	0.0144	0.0169	0.0095	0.0089	0.0070	0.0093	0.0076	0.0092	0.0175	0.0062	0.0091	0.0089	0.0088	0.0077	0.0092	0.0094	0.0134	0.0103	0.0100	0.0101	0.0098	0.0087	0.0082	0.0052	0.0087	0.0103	0.0072	0.0154	0.0092	0.0062
Cer 24:1	0.0226	0.0238	0.0200	0.0272	0.0268	0.0341	0.0389	0.0307	0.0325	0.0273	0.0192	0.0429	0.0258	0.0266	0.0618	0.1031	0.0289	0.0270	0.0292	0.0330	0.0274	0.0398	0.0873	0.0396	0.0734	0.0484	0.0523	0.0452	0.0558	0.0588	0.1007	0.0558	0.0487	0.0455	0.0658	0.0461	0.0646	0.0308	0.0598	0.0646	0.0306	0.1325	0.0527	0.0168
SM 16:0	4.9217	5.9280	5.4396	5.5146	4.2633	6.7817	7.7988	4.2059	4.4933	4.7361	6.0186	5.5350	5.7758	5.6153	5.0401	7.5283	4.8140	4.3475	4.9312	6.7501	5.7599	6.1828	7.4304	6.6493	6.4075	7.3089	4.4835	6.6188	6.6284	4.8388	7.9494	4.6929	4.8745	5.4147	8.2039	6.3897	5.7329	3.6814	5.4588	4.3821	4.9472	6.8288	6.2871	5.2997
SM 18:0	25.2242	27.0182	25.9138	25.6589	26.0884	29.1721	25.1526	23.1584	27.3241	26.7520	25.4849	27.6827	27.8674	26.4722	28.6557	30.1162	26.5646	24.3630	25.4397	27.5524	26.8239	26.5173	21.0246	27.7098	22.0681	23.7736	21.0988	23.8742	20.8251	19.1401	23.7883	20.1053	24.4960	23.7830	27.1438	24.6285	22.2014	20.0347	23.6499	21.6004	21.9394	29.9276	25.9641	20.0575
SM 18:1	3.3343	3.3786	3.5296	3.3017	3.2645	3.3257	3.2342	2.7689	3.4595	3.4431	3.4529	3.3895	3.5764	3.4308	3.0639	3.6058	3.5934	3.0031	3.3836	3.7147	3.5582	3.6335	2.4208	3.2873	2.7067	3.2332	2.7292	2.9609	2.9409	2.2916	2.9174	2.1594	3.1053	3.3638	3.9016	3.2994	2.5919	2.4119	2.8317	2.5713	2.6993	2.8186	2.8326	2.6383
SM 20:0	5.1045	5.2655	4.3217	5.5279	3.2538	4.6042	5.2481	4.4303	4.8148	5.4049	4.4547	5.9251	4.3425	5.7209	7.2921	4.4351	5.0651	3.6667	4.7737	4.8496	4.5948	7.2302	8.1515	6.8751	7.8818	8.8849	6.5830	7.8481	7.7412	7.0325	7.4251	6.8404	7.0599	7.5962	8.5719	8.2587	8.9845	4.9434	5.1480	6.6435	5.8674	10.5813	5.9543	6.4408
SM 22:0	1.4101	1.5058	1.3096	1.6187	1.1334	1.7694	1.9286	1.3733	1.4283	1.3407	1.5257	1.6332	1.5501	1.5412	1.9766	3.4356	1.7238	1.1526	1.2997	1.7870	1.6777	1.9884	3.7873	1.9094	3.0204	2.7906	1.9224	2.2876	2.5671	3.6471	3.7279	2.0658	2.1010	2.2253	3.3570	2.3539	2.7797	1.7318	1.9289	2.6672	1.7828	4.0689	2.4912	1.8591
SM 24:0	1.1284	1.1416	1.0042	1.3435	0.9250	1.4823	1.5628	1.1852	1.3528	1.2040	1.2268	1.3411	1.2770	1.2576	1.9489	3.5075	1.4053	1.0228	0.8988	1.4434	1.4544	1.4884	3.0301	1.5577	2.5134	2.1326	1.8342	1.9793	2.1260	2.1492	2.7200	1.8101	1.9771	2.0610	2.6093	1.9634	2.5124	1.7808	1.4533	2.3965	1.5606	3.7968	2.0122	1.4015
SM 24:1	2.0400	2.1725	1.5726	2.4239	1.5015	2.1899	2.1463	1.7552	2.5198	2.0784	1.7415	2.4211	1.7022	1.9749	4.0877	5.4281	2.4358	1.6186	1.6900	2.6331	2.4701	2.5880	5.7303	2.8194	5.2838	3.8701	3.7307	3.4398	4.2092	4.0935	6.2709	3.0000	3.8294	4.2386	5.5083	3.6282	4.9204	3.6042	2.9565	4.7465	2.6903	7.2119	4.0773	2.5854
SM 26:0	0.0156	0.0171	0.0175	0.0177	0.0161	0.0246	0.0359	0.0266	0.0187	0.0167	0.0253	0.0170	0.0265	0.0265	0.0289	0.0461	0.0232	0.0161	0.0098	0.0244	0.0203	0.0285	0.1026	0.0271	0.0486	0.0484	0.0357	0.0492	0.0508	0.0445	0.0757	0.0384	0.0424	0.0442	0.0536	0.0302	0.0526	0.0313	0.0212	0.0544	0.0335	0.1211	0.0312	0.0292
SM 26:1	0.0439	0.0450	0.0435	0.0533	0.0411	0.0533	0.0843	0.0633	0.0596	0.0540	0.0617	0.0558	0.0588	0.0732	0.0982	0.1564	0.0672	0.0403	0.0315	0.0616	0.0538	0.0847	0.3452	0.0689	0.2078	0.1666	0.1282	0.1481	0.1765	0.1603	0.3143	0.1324	0.1395	0.1387	0.2006	0.1099	0.2187	0.1060	0.0871	0.2132	0.1116	0.5208	0.1087	0.1057
dhSM 16:0	0.1369	0.2064	0.2031	0.1889	0.1559	0.2661	0.3199	0.1695	0.1291	0.1759	0.2350	0.2338	0.3100	0.2025	0.1934	0.2731	0.1895	0.1683	0.1427	0.2559	0.2142	0.1695	0.2120	0.2138	0.1558	0.2590	0.0979	0.2055	0.1678	0.1131	0.1707	0.1155	0.1530	0.1503	0.2255	0.2060	0.1406	0.0665	0.1040	0.1016	0.1470	0.1469	0.1593	0.1658
dhSM 16:1	0.0278	0.0217	0.0245	0.0187	0.0252	0.0333	0.0365	0.0188	0.0215	0.0300	0.0316	0.0379	0.0372	0.0354	0.0238	0.0311	0.0264	0.0217	0.0191	0.0282	0.0276	0.0241	0.0317	0.0386	0.0324	0.0687	0.0208	0.0385	0.0281	0.0299	0.0308	0.0193	0.0325	0.0343	0.0343	0.0367	0.0204	0.0149	0.0233	0.0265	0.0206	0.0244	0.0274	0.0318
dhSM 18:0	1.7988	2.3175	2.0205	1.6092	1.6859	2.0971	2.4275	1.2700	1.7167	1.8058	2.3249	3.3325	2.5422	1.8135	1.8920	2.8802	2.0617	1.9848	1.9205	2.7590	2.7790	1.9062	1.3500	2.9017	1.8223	2.9117	0.9481	1.8097	2.1355	1.3281	2.3786	0.9801	1.5384	1.6129	3.0178	2.7354	1.7337	0.7614	1.4359	1.2998	1.2145	2.6292	2.0770	1.6629
dhSM 18:1	0.6260	0.7747	0.6452	0.4706	0.5154	0.6172	0.8415	0.4725	0.6501	0.6526	0.8258	1.0820	0.8702	0.6034	0.5593	0.8717	0.7461	0.5704	0.6626	0.9233	0.8719	0.7058	0.5289	0.9795	0.8690	1.3259	0.4180	0.7292	0.8674	0.5297	0.8227	0.4233	0.6308	0.6497	1.2171	1.1820	0.6696	0.3232	0.6141	0.5067	0.5156	0.8761	0.9450	0.6912
dhSM 20:0	0.2097	0.2488	0.1419	0.1874	0.1203	0.1530	0.2462	0.1796	0.2761	0.2171	0.1728	0.3870	0.2011	0.2091	0.3102	0.4046	0.2217	0.1401	0.1811	0.2290	0.2093	0.3335	0.4094	0.3585	0.3852	0.5048	0.2028	0.3233	0.3900	0.2851	0.4297	0.1883	0.2479	0.2904	0.4774	0.4416	0.3792	0.1524	0.2353	0.3232	0.2067	0.6303	0.2801	0.2710
dhSM 20:1	0.0585	0.0624	0.0471	0.0378	0.0389	0.0475	0.0714	0.0430	0.0587	0.0557	0.0582	0.0844	0.0611	0.0491	0.0601	0.1007	0.0593	0.0421	0.0489	0.0669	0.0673	0.0704	0.0777	0.0893	0.0984	0.1240	0.0468	0.0747	0.1000	0.0681	0.0978	0.0475	0.0614	0.0645	0.1369	0.1271	0.0896	0.0368	0.0562	0.0699	0.0493	0.1265	0.0754	0.0695
dhSM 22:0	0.0883	0.1009	0.0597	0.0971	0.0688	0.0967	0.1125	0.0687	0.1065	0.0784	0.0952	0.1182	0.0910	0.0829	0.1109	0.8742	0.1051	0.0745	0.0770	0.1192	0.1527	0.1387	0.2972	0.1608	0.3179	0.2575	0.1425	0.1434	0.2314	0.2324	0.6602	0.1121	0.1433	0.1717	0.4668	0.2109	0.3183	0.1221	0.1784	0.3009	0.1052	0.6561	0.2063	0.1197
dhSM 22:1	0.0490	0.0574	0.0322	0.0540	0.0379	0.0509	0.0474	0.0344	0.0682	0.0425	0.0447	0.0639	0.0435	0.0326	0.0613	0.4233	0.0546	0.0399	0.0449	0.0644	0.0745	0.0798	0.1743	0.0867	0.1988	0.1372	0.0798	0.0718	0.1302	0.1289	0.4617	0.0519	0.0762	0.0956	0.3033	0.1206	0.1864	0.0647	0.1161	0.1780	0.0526	0.3545	0.1330	0.0583
dhSM 24:0	0.0572	0.0590	0.0421	0.0688	0.0483	0.0736	0.0775	0.0477	0.0759	0.0508	0.0642	0.0731	0.0660	0.0496	0.0776	0.6083	0.0679	0.0475	0.0468	0.0804	0.0987	0.0919	0.2067	0.0873	0.2215	0.1445	0.0868	0.0945	0.1325	0.1432	0.4129	0.0694	0.1013	0.1243	0.2477	0.1429	0.1969	0.0739	0.1284	0.2083	0.0630	0.5284	0.1226	0.0581
dhSM 24:1	0.2887	0.3178	0.2305	0.3517	0.2681	0.3616	0.4471	0.2524	0.3976	0.2760	0.3667	0.3728	0.3485	0.2633	0.5185	3.1056	0.4253	0.2752	0.2655	0.4155	0.5161	0.5675	1.0207	0.4933	1.1117	0.7582	0.4446	0.5092	0.6790	0.6956	2.5147	0.3444	0.4846	0.6186	1.6261	0.7340	0.9775	0.4013	0.6444	1.0419	0.3828	2.4688	0.7668	0.4100
dhSM 26:1	0.0042	0.0063	0.0061	0.0056	0.0058	0.0077	0.0139	0.0084	0.0065	0.0058	0.0116	0.0050	0.0119	0.0108	0.0119	0.0278	0.0100	0.0072	0.0043	0.0093	0.0068	0.0111	0.0199	0.0084	0.0098	0.0126	0.0065	0.0114	0.0113	0.0078	0.0247	0.0061	0.0094	0.0079	0.0156	0.0068	0.0102	0.0056	0.0057	0.0114	0.0096	0.0255	0.0090	0.0085
dhSM 26:2	0.0049	0.0082	0.0062	0.0074	0.0069	0.0083	0.0142	0.0087	0.0065	0.0064	0.0119	0.0052	0.0129	0.0093	0.0114	0.0277	0.0096	0.0080	0.0044	0.0112	0.0077	0.0107	0.0140	0.0081	0.0099	0.0124	0.0060	0.0113	0.0110	0.0074	0.0239	0.0067	0.0096	0.0097	0.0167	0.0070	0.0104	0.0049	0.0048	0.0100	0.0090	0.0205	0.0091	0.0089
HexCer 16:0	0.0034	0.0040	0.0022	0.0042	0.0028	0.0025	0.0030	0.0029	0.0074	0.0038	0.0021	0.0067	0.0024	0.0025	0.0112	0.0152	0.0066	0.0063	0.0079	0.0092	0.0066	0.0108	0.0148	0.0055	0.0157	0.0078	0.0087	0.0054	0.0092	0.0117	0.0206	0.0038	0.0055	0.0060	0.0153	0.0050	0.0104	0.0048	0.0135	0.0155	0.0030	0.0227	0.0097	0.0035
HexCer 16:1	0.0011	0.0011	0.0016	0.0013	0.0016	0.0014	0.0013	0.0016	0.0017	0.0016	0.0013	0.0016	0.0015	0.0013	0.0031	0.0020	0.0019	0.0024	0.0022	0.0024	0.0022	0.0019	0.0018	0.0019	0.0021	0.0022	0.0022	0.0023	0.0022	0.0011	0.0013	0.0015	0.0017	0.0014	0.0018	0.0016	0.0017	0.0011	0.0015	0.0014	0.0014	0.0016	0.0014	0.0011
HexCer 18:0	0.5035	0.5113	0.3469	0.5861	0.3928	0.3502	0.3985	0.4367	0.7033	0.4704	0.3285	0.7865	0.3824	0.3726	0.7851	1.4837	0.5862	0.5161	0.6152	0.6563	0.5866	0.8183	1.1647	0.5246	1.0642	0.6241	0.6440	0.4892	0.7576	1.1019	1.7376	0.5633	0.6277	0.6737	1.1504	0.6308	0.9892	0.5927	1.2475	1.4319	0.4572	2.2233	0.9683	0.4172
HexCer 18:1	0.0236	0.0215	0.0158	0.0240	0.0190	0.0165	0.0188	0.0204	0.0244	0.0194	0.0154	0.0260	0.0162	0.0178	0.0233	0.0421	0.0213	0.0207	0.0235	0.0234	0.0211	0.0278	0.0439	0.0221	0.0432	0.0256	0.0275	0.0204	0.0272	0.0398	0.0572	0.0213	0.0263	0.0269	0.0417	0.0233	0.0356	0.0266	0.0394	0.0450	0.0200	0.0687	0.0343	0.0198
HexCer 20:0	0.1091	0.1162	0.0606	0.1666	0.0816	0.0790	0.0858	0.1108	0.1945	0.1138	0.0544	0.1938	0.0689	0.0846	0.2255	0.3240	0.1578	0.1215	0.1390	0.1493	0.1197	0.1997	0.4041	0.1565	0.3680	0.1869	0.2403	0.1433	0.2405	0.2914	0.4345	0.1627	0.1922	0.2025	0.3723	0.1780	0.3414	0.2058	0.3877	0.4881	0.1322	0.4692	0.2656	0.1065
HexCer 20:1	0.0053	0.0066	0.0026	0.0079	0.0042	0.0035	0.0045	0.0060	0.0106	0.0052	0.0023	0.0101	0.0033	0.0037	0.0122	0.0122	0.0066	0.0054	0.0066	0.0071	0.0053	0.0106	0.0254	0.0095	0.0217	0.0104	0.0125	0.0079	0.0155	0.0149	0.0286	0.0081	0.0120	0.0121	0.0258	0.0093	0.0203	0.0125	0.0211	0.0270	0.0065	0.0274	0.0161	0.0056
HexCer 22:0	0.3125	0.2853	0.1642	0.4326	0.2413	0.2088	0.2372	0.3086	0.5169	0.3087	0.1543	0.4846	0.1885	0.2187	0.5591	1.1619	0.3564	0.3474	0.3259	0.3903	0.3796	0.5501	1.0513	0.4007	0.9423	0.5071	0.7233	0.4399	0.6653	0.5803	1.2649	0.5295	0.5857	0.6414	0.9496	0.5303	0.9393	0.5861	0.9014	1.1301	0.4092	1.3777	0.7289	0.2906
HexCer 22:1	0.1209	0.1310	0.0642	0.1827	0.0935	0.0923	0.0778	0.1084	0.2207	0.1060	0.0483	0.1901	0.0602	0.0766	0.2109	0.2322	0.1115	0.1115	0.1217	0.1503	0.1201	0.2065	0.4778	0.2138	0.4569	0.1982	0.2937	0.1645	0.2800	0.2647	0.6556	0.1414	0.2434	0.2513	0.5024	0.2004	0.3920	0.2343	0.4080	0.4796	0.1145	0.5085	0.3163	0.1030
HexCer 24:0	0.4732	0.3790	0.2131	0.5865	0.3585	0.2911	0.3272	0.4472	0.7340	0.4547	0.2455	0.6658	0.2480	0.3241	1.0419	2.2930	0.5019	0.4954	0.4780	0.5488	0.6314	0.8217	1.3866	0.5316	1.2583	0.7247	0.9733	0.6854	0.9088	0.7268	1.7897	0.8982	0.8600	0.9687	1.2861	0.7944	1.2750	0.8316	1.1295	1.4280	0.6073	2.2136	1.0501	0.4367
HexCer 24:1	1.7868	1.6815	0.8447	2.5401	1.4228	1.3240	1.2831	1.7895	3.1233	1.7314	0.8846	2.7956	0.9770	1.2827	4.1456	5.2875	1.9572	1.6928	1.8184	2.1739	2.0463	3.3271	6.4123	2.4624	6.0496	3.2903	4.3336	2.8592	4.2535	3.3500	8.7400	3.0878	3.5940	3.9249	6.0533	3.0670	5.5370	3.2355	5.1416	5.8765	2.2962	9.1800	4.5508	1.7595
HexCer 26:0	0.0032	0.0023	0.0015	0.0034	0.0023	0.0013	0.0024	0.0021	0.0045	0.0026	0.0019	0.0037	0.0015	0.0022	0.0059	0.0171	0.0031	0.0038	0.0032	0.0035	0.0046	0.0049	0.0133	0.0055	0.0085	0.0049	0.0063	0.0047	0.0067	0.0070	0.0115	0.0070	0.0057	0.0075	0.0088	0.0063	0.0094	0.0060	0.0084	0.0101	0.0035	0.0168	0.0067	0.0025
HexCer 26:1	0.0091	0.0078	0.0038	0.0117	0.0072	0.0062	0.0061	0.0085	0.0151	0.0086	0.0042	0.0141	0.0051	0.0058	0.0212	0.0409	0.0095	0.0085	0.0091	0.0109	0.0113	0.0163	0.0329	0.0123	0.0289	0.0149	0.0206	0.0155	0.0207	0.0200	0.0440	0.0191	0.0191	0.0192	0.0307	0.0162	0.0289	0.0160	0.0255	0.0319	0.0125	0.0589	0.0228	0.0086
LacCer 16:0	0.0042	0.0041	0.0027	0.0055	0.0031	0.0048	0.0029	0.0027	0.0080	0.0052	0.0025	0.0089	0.0037	0.0040	0.0048	0.0167	0.0064	0.0064	0.0073	0.0080	0.0069	0.0116	0.0130	0.0046	0.0124	0.0072	0.0081	0.0063	0.0093	0.0112	0.0164	0.0059	0.0069	0.0060	0.0076	0.0058	0.0080	0.0050	0.0124	0.0138	0.0055	0.0222	0.0086	0.0041
LacCer 18:0	0.1821	0.1703	0.1398	0.2204	0.1561	0.1562	0.1659	0.1563	0.2274	0.1975	0.1333	0.3290	0.1638	0.1787	0.1218	0.4202	0.1829	0.1461	0.2036	0.1975	0.1763	0.2739	0.4287	0.2149	0.3970	0.2949	0.2903	0.2691	0.3372	0.4455	0.3820	0.2758	0.2899	0.3274	0.3351	0.2760	0.3556	0.2109	0.4640	0.5146	0.2501	0.7276	0.3331	0.1939
LacCer 20:0	0.0058	0.0066	0.0045	0.0071	0.0056	0.0062	0.0060	0.0061	0.0079	0.0068	0.0059	0.0089	0.0071	0.0066	0.0033	0.0090	0.0065	0.0062	0.0071	0.0065	0.0064	0.0063	0.0116	0.0076	0.0109	0.0111	0.0087	0.0103	0.0104	0.0105	0.0112	0.0097	0.0093	0.0087	0.0096	0.0107	0.0096	0.0062	0.0121	0.0133	0.0085	0.0155	0.0126	0.0060
LacCer 22:0	0.0049	0.0069	0.0062	0.0068	0.0072	0.0085	0.0086	0.0067	0.0069	0.0085	0.0073	0.0088	0.0082	0.0076	0.0041	0.0103	0.0092	0.0063	0.0074	0.0068	0.0068	0.0086	0.0136	0.0068	0.0080	0.0094	0.0091	0.0088	0.0091	0.0089	0.0110	0.0094	0.0090	0.0079	0.0103	0.0086	0.0070	0.0055	0.0088	0.0120	0.0101	0.0135	0.0092	0.0066
LacCer 24:0	0.0056	0.0065	0.0058	0.0085	0.0071	0.0094	0.0086	0.0079	0.0090	0.0072	0.0063	0.0091	0.0091	0.0087	0.0036	0.0189	0.0085	0.0063	0.0072	0.0085	0.0075	0.0114	0.0223	0.0087	0.0147	0.0120	0.0113	0.0124	0.0124	0.0156	0.0215	0.0112	0.0113	0.0111	0.0148	0.0097	0.0136	0.0084	0.0136	0.0170	0.0111	0.0255	0.0126	0.0069
LacCer 24:1	0.0099	0.0096	0.0085	0.0129	0.0097	0.0142	0.0103	0.0114	0.0149	0.0116	0.0081	0.0165	0.0098	0.0104	0.0079	0.0312	0.0113	0.0098	0.0107	0.0146	0.0132	0.0181	0.0397	0.0125	0.0306	0.0211	0.0203	0.0153	0.0215	0.0302	0.0423	0.0177	0.0190	0.0194	0.0313	0.0166	0.0231	0.0142	0.0284	0.0292	0.0137	0.0569	0.0248	0.0095
PC 30:0	10.0948	10.5655	10.2235	11.3088	8.6677	10.0330	11.2047	8.8400	9.5719	8.2867	10.5211	10.7025	11.0299	10.8793	10.7443	10.1451	9.1993	10.4762	10.2931	9.6042	9.8234	10.5519	11.3859	9.6761	12.9709	10.6404	11.0286	9.6474	12.0076	10.7269	11.0206	12.2398	12.5307	14.3932	12.5139	11.0132	13.9231	9.9200	10.4229	10.2021	12.4619	9.9591	10.7681	10.6810
PC 32:0	45.1712	48.8426	57.8862	49.1318	51.4347	50.3705	56.2537	46.8607	54.9271	46.2663	49.9440	62.6831	55.7759	56.6678	60.7089	57.2968	55.2853	54.7246	56.7076	53.6069	58.5717	55.2649	50.3774	46.8615	45.9892	40.0578	52.1844	39.4381	49.4272	44.7510	49.3375	51.0449	51.2349	55.9585	45.9185	48.0545	49.6627	48.4156	45.1662	44.3406	51.7262	49.2614	44.6560	44.7325
PC 32:1	7.3421	9.2823	9.6494	7.8705	8.7416	8.9544	9.7753	7.1674	8.2207	6.9473	8.4462	8.5202	8.8782	8.3271	8.4217	9.3371	8.5487	7.4985	8.8900	9.3624	9.5654	7.8755	7.1070	6.9162	7.8426	6.8921	8.4942	7.0503	8.7995	7.1955	9.5476	7.5650	8.2303	8.9224	8.8705	7.6544	8.3507	7.5868	7.3361	6.4204	8.0942	8.7300	7.5691	7.2859
PC 34:0	12.6420	13.2595	14.7700	14.6727	14.2521	13.2490	14.7628	13.9177	16.6405	13.0894	12.0476	16.8528	14.4287	16.3537	18.0228	17.4361	15.8849	14.2913	15.7525	14.1034	14.4131	15.1882	14.4912	12.8755	12.3988	11.4755	13.9630	11.5184	14.0724	12.7302	12.2442	15.0127	14.5246	15.1971	13.4461	13.3475	12.9382	13.5958	14.2970	12.3989	14.4702	14.0178	11.2686	12.9051
PC 34:1	25.9132	30.7560	36.0012	30.8700	36.2144	30.0647	33.8665	33.4523	38.9882	30.3132	33.0540	36.1678	37.6887	36.0739	41.3395	42.6196	35.7188	32.6736	34.2639	30.7326	37.6216	34.9834	30.8879	30.3977	28.3896	26.3560	31.1634	27.3329	31.3915	32.8517	32.9387	34.7896	32.3409	35.6220	30.0022	29.0579	30.5527	31.9477	31.6957	28.7902	32.7870	32.8783	29.1371	27.8880
PC 34:2	2.4219	2.6054	3.0348	2.5895	3.1435	2.7266	2.9179	3.0385	3.0306	2.1574	2.6657	2.6024	2.7155	2.7676	3.3027	3.1324	3.2542	2.6785	2.8544	3.2445	2.9177	2.8149	2.3968	2.3582	2.1084	2.1656	2.6500	2.3623	2.4251	2.2199	2.5679	2.8636	3.0575	2.9223	2.2370	2.4757	2.8373	2.6533	2.4620	2.0276	2.6685	2.2990	2.4765	2.7412
PC 36:0	1.7505	1.8436	1.8922	1.8272	2.0191	1.6434	2.0459	1.9352	2.3967	1.8065	1.8012	2.4045	2.0205	2.2721	2.6186	3.2345	2.3247	2.0958	2.3247	2.0753	1.9921	2.3149	2.1392	1.8564	2.0098	1.7284	2.0016	1.6148	2.0062	1.9841	2.3730	2.1243	2.1848	2.1941	2.1294	1.8074	1.8574	1.7692	2.0121	1.7851	1.8686	2.4178	2.0263	1.8196
PC 36:1	16.4877	15.4159	18.4736	17.4524	17.3977	16.5785	19.7779	17.0808	18.3686	17.0381	16.5560	21.6498	17.7629	18.3578	24.6205	30.3018	19.6708	17.3745	19.2481	17.8197	19.1902	18.4232	18.8801	16.6313	18.1151	15.7026	16.8854	15.4912	19.3856	17.7917	21.0411	18.1751	17.5142	19.1221	16.5076	17.7834	19.5248	17.8621	16.8147	16.9163	16.8489	21.3018	16.6865	16.4280
PC 36:2	7.1905	6.9382	7.8430	7.0281	7.5412	7.3684	7.8850	7.2665	9.2631	6.6859	6.6359	8.8366	8.1752	8.7065	12.0160	13.8482	7.9739	7.5541	8.9408	8.1126	8.0076	9.1119	8.1987	7.2624	7.5364	7.2937	8.1055	7.0741	8.3335	7.4968	8.4445	9.1267	8.1934	8.6093	7.4051	8.2467	8.8220	6.8622	7.2491	7.0763	8.2545	8.3661	7.5498	8.0278
PC 36:3	3.9755	4.1709	4.1118	4.0257	4.3032	4.1983	4.8124	4.3057	4.3316	3.6241	3.6366	4.4651	4.2783	4.8446	5.3781	6.3166	4.4232	3.8867	4.5915	4.6755	4.5144	4.4745	3.5815	3.8989	3.4896	3.9279	3.7457	3.6641	3.9192	3.4912	3.3121	4.2347	4.1132	4.2539	4.1963	4.1215	4.0086	3.7381	3.4877	3.0399	4.2079	2.5451	3.8358	4.3914
PC 36:4	19.0590	16.7564	21.2467	17.8660	20.6624	18.7954	23.5739	19.7057	20.9487	18.3625	19.1713	22.4995	23.0834	20.6240	24.6229	29.0839	21.9076	19.0001	21.0450	21.1542	21.5976	20.3370	16.8652	20.8576	17.9764	18.1771	17.4859	14.7323	18.8231	16.0353	17.0638	19.9920	19.5008	20.8495	20.2855	20.8174	17.9268	18.6824	18.4997	13.9136	19.6124	11.5585	17.6213	21.5915
PC 38:0	0.0925	0.0957	0.0823	0.1066	0.1063	0.0960	0.1012	0.1036	0.1409	0.0887	0.0774	0.1317	0.0928	0.1152	0.1764	0.4902	0.1159	0.0929	0.1060	0.1038	0.1086	0.1375	0.2586	0.1055	0.2179	0.1349	0.1704	0.1097	0.1818	0.2047	0.3689	0.1702	0.1537	0.1784	0.1910	0.1292	0.2215	0.1744	0.1781	0.2088	0.1294	0.4177	0.1548	0.1109
PC 38:1	0.3551	0.3568	0.3331	0.4530	0.4288	0.4260	0.3875	0.4451	0.5937	0.3658	0.3012	0.5591	0.3570	0.4872	0.7031	1.7291	0.4667	0.3872	0.4362	0.3945	0.4218	0.5469	1.0736	0.4420	0.9772	0.5329	0.8232	0.4558	0.8024	0.8794	1.5942	0.7041	0.6134	0.7700	0.8603	0.6072	0.8996	0.7381	0.8154	0.9093	0.4665	1.8272	0.6384	0.4754
PC 38:2	0.4812	0.4335	0.4714	0.5702	0.5434	0.4852	0.5194	0.5087	0.6787	0.4384	0.4117	0.6731	0.5210	0.5917	0.8653	1.6274	0.6063	0.4745	0.5469	0.5271	0.5508	0.6605	1.2503	0.5162	0.9584	0.6188	0.8640	0.4995	0.8942	0.9540	1.7666	0.7755	0.7109	0.8192	0.8577	0.6543	0.9832	0.7559	0.8559	1.0397	0.5878	1.8541	0.7960	0.5460
PC 38:3	1.9792	1.9902	2.2773	1.9051	2.4410	1.9994	2.3068	2.1009	2.5746	1.8955	1.9249	2.5259	2.3840	2.4506	2.7286	3.2034	2.5677	2.1484	2.3471	2.2615	2.3994	2.2373	1.9896	1.9626	1.7856	1.7087	1.8334	1.5488	1.9930	1.8064	2.0801	1.7529	1.9979	2.1827	2.1943	2.0831	1.7896	2.0038	1.9599	1.5776	1.8237	1.6346	1.9621	1.7435
PC 38:4	12.3358	13.6386	14.1845	12.8323	15.7380	12.6102	16.6393	12.7793	15.9541	11.5589	12.5348	16.0282	15.3535	14.8273	16.9718	17.3983	14.9392	14.9778	15.0983	14.8790	14.2322	16.2051	10.3363	12.2293	10.4895	10.3048	11.7900	10.1454	11.6983	9.8644	12.7502	10.1188	11.0521	12.6253	13.2158	11.9285	10.4484	11.1178	12.0204	8.2149	10.3486	8.5528	11.3280	12.3520
PC 38:5	6.0604	6.2468	6.8448	5.3473	8.2965	6.3705	7.1494	5.8903	7.6843	5.1848	5.7757	6.9714	6.3351	6.5151	7.9768	7.8370	6.4256	5.8471	7.1919	7.8390	7.2126	6.9592	5.7079	6.3547	5.8479	5.9805	6.1314	5.7174	6.6024	5.5173	6.5088	6.1905	5.8757	7.0803	6.9266	6.7014	6.2544	6.0978	6.7190	3.8400	6.3227	4.9757	6.3671	6.3833
PC 38:6	7.8376	6.8336	7.2152	8.0208	8.6377	6.6266	8.5074	8.5019	10.0852	7.2396	6.0071	8.8909	7.7741	9.1418	12.1548	10.4937	8.6949	6.9822	7.6810	7.8310	7.6476	10.0619	11.5769	8.2014	9.8532	9.5821	11.1849	10.0069	11.9082	10.6522	10.0043	16.1073	11.9840	12.5504	9.4844	10.4677	12.5146	9.4554	8.2435	8.7925	12.1051	11.2324	7.9756	10.1471
PC 40:4	1.1692	1.1903	1.1701	1.0839	1.2024	1.0948	1.4443	1.1677	1.4709	1.0886	1.1163	1.3103	1.5323	1.4340	1.9122	1.3504	1.3513	1.2284	1.3185	1.1794	1.1936	1.3863	1.0722	1.2222	1.1232	1.1544	1.0891	0.8839	1.1205	1.0051	1.1859	1.0432	1.0975	1.1927	1.1959	1.1024	1.0572	1.0304	1.0533	0.8265	0.9284	1.0950	1.2032	1.1423
PC 40:5	1.8760	1.7791	1.9625	1.8361	2.2302	1.9976	2.2456	1.8518	2.3104	1.7871	1.5690	2.4344	2.2248	2.3966	3.0685	2.5240	1.9740	1.7719	2.1140	2.1767	2.0061	2.2399	2.0441	1.9864	2.0853	1.8064	2.1107	1.6384	1.9436	1.9178	2.0991	2.2939	2.1205	2.3422	2.0233	2.2115	2.1444	1.9220	1.8898	1.3763	1.8236	2.0000	1.9910	1.9314
PC 40:6	9.1037	7.6898	8.8060	8.2571	9.0396	7.8590	9.6469	7.8472	11.0271	7.6207	7.4896	10.3252	10.6167	11.2362	13.9826	10.7474	9.4723	7.8933	8.8501	8.9900	8.7404	10.2141	11.0694	9.1733	10.2027	9.8236	11.1086	8.5003	11.7752	8.9612	10.3146	12.5384	10.4109	11.8466	9.7565	10.8453	10.9327	9.5136	8.3112	6.8257	9.3906	10.8742	8.3159	10.4839
PC 40:7	4.9827	4.8799	4.9228	4.6539	4.5689	4.6181	5.8658	4.3860	5.5272	3.9438	4.2147	5.2929	5.2327	5.6990	6.9834	5.6609	5.1352	4.8314	5.1392	5.5161	5.8482	5.7691	6.1049	4.9667	5.7572	6.7869	5.8885	5.5179	6.6426	5.4976	5.6585	7.3503	6.2602	7.0660	6.4545	6.9085	6.3798	5.2590	5.0063	4.4210	5.9229	5.5502	6.4633	6.6718
PC 42:5	0.1632	0.1710	0.1753	0.1441	0.1596	0.1647	0.1960	0.1411	0.1979	0.1290	0.1697	0.1983	0.1783	0.1585	0.2282	0.2572	0.1774	0.1505	0.1877	0.2120	0.2191	0.2138	0.2069	0.1722	0.2086	0.1869	0.1533	0.1434	0.1812	0.2143	0.2878	0.1561	0.1703	0.1809	0.2347	0.2208	0.2043	0.1444	0.1858	0.1466	0.1403	0.2248	0.1999	0.1710
PC 42:6	0.2296	0.2467	0.2581	0.2243	0.2126	0.2023	0.2892	0.2095	0.2670	0.2007	0.2286	0.3089	0.2440	0.2736	0.3155	0.3713	0.2752	0.2093	0.3000	0.3202	0.3293	0.3107	0.3119	0.2718	0.2932	0.3540	0.2300	0.2509	0.3340	0.3331	0.3274	0.2955	0.2775	0.2966	0.3453	0.3588	0.3089	0.1805	0.2228	0.2107	0.2617	0.3701	0.3511	0.3148
PC 42:7	0.4670	0.5156	0.5301	0.4750	0.4030	0.4066	0.5911	0.3652	0.5094	0.4081	0.4722	0.6732	0.4717	0.5476	0.6583	1.2060	0.6407	0.4444	0.6300	0.6584	0.6562	0.8173	0.7841	0.5734	0.8647	0.9897	0.5585	0.6818	0.8971	0.7810	0.8740	0.9284	0.7440	0.7091	0.9505	1.0945	0.9297	0.4771	0.5898	0.6209	0.6680	1.4805	0.9115	0.8329
PCe 30:0	0.1539	0.1564	0.1544	0.1916	0.1329	0.1474	0.1695	0.1324	0.1444	0.1263	0.1483	0.1515	0.1313	0.1450	0.2003	0.1652	0.1532	0.1394	0.1535	0.1416	0.1478	0.1572	0.1727	0.1586	0.1769	0.1489	0.1495	0.1449	0.1923	0.1610	0.1859	0.1708	0.2064	0.1910	0.1960	0.1869	0.2090	0.1978	0.1417	0.1466	0.1560	0.1555	0.1619	0.1750
PCe 32:0	1.8314	1.9111	2.3108	2.3800	2.1030	2.0347	1.9769	1.8392	1.7167	1.6555	1.7830	2.0465	2.0158	2.6309	2.9199	2.5152	1.8298	1.9833	2.2511	1.9952	2.0471	2.1083	2.0440	2.0816	1.9054	1.9472	2.2085	1.9335	2.0083	1.8967	1.9784	2.0026	2.3423	2.7145	2.0972	2.1105	3.0400	1.9448	1.6273	1.7700	2.2281	1.8099	1.8513	2.0172
PCe 32:1	0.5532	0.6112	0.7621	0.6144	0.7778	0.6837	0.7219	0.5513	0.5526	0.5039	0.6701	0.7115	0.6297	0.7220	0.6723	0.7163	0.6110	0.5988	0.8302	0.6407	0.6930	0.6660	0.4618	0.5754	0.4614	0.4481	0.5224	0.4086	0.4993	0.4062	0.5531	0.5259	0.5469	0.5732	0.5940	0.5292	0.5213	0.5216	0.6013	0.4105	0.4977	0.4185	0.5721	0.5405
PCe 34:0	0.7358	0.6995	0.8299	0.8902	0.8181	0.7950	0.8510	0.8425	0.7492	0.6492	0.6894	0.8783	0.7347	0.9808	1.2262	0.8425	0.7899	0.7228	0.8455	0.8154	0.7933	0.9266	0.8765	0.8485	0.7711	0.9525	0.8375	0.7759	0.8176	0.7857	0.7698	0.8876	0.8710	0.9759	0.8212	1.0140	1.2243	0.7650	0.7389	0.7471	0.9485	0.8488	0.7275	0.8695
PCe 34:1	1.0672	1.1409	1.2327	1.2945	1.3094	1.2372	1.2176	1.0887	1.1338	0.9983	1.0949	1.2613	1.1674	1.2947	1.4096	1.6112	1.2834	1.1259	1.2081	1.2900	1.2807	1.2283	0.9416	1.1346	0.9239	0.9648	1.0441	0.9790	1.0764	0.9393	1.1194	1.2192	1.1519	1.2555	1.2016	1.2889	1.2342	1.0327	1.0248	0.8238	1.1247	0.9582	0.9637	1.1395
PCe 34:2	0.1290	0.1292	0.1592	0.1534	0.1583	0.1553	0.1670	0.1434	0.1497	0.1184	0.1206	0.1612	0.1490	0.1473	0.1869	0.2924	0.1547	0.1483	0.1553	0.1553	0.1595	0.1658	0.1516	0.1534	0.1335	0.1351	0.1353	0.1181	0.1573	0.1356	0.2365	0.1498	0.1353	0.1624	0.1699	0.1499	0.1621	0.1401	0.1469	0.1365	0.1451	0.2101	0.1639	0.1538
PCe 36:0	0.6128	0.6546	0.5089	0.7180	0.6733	0.6507	0.4948	0.5732	0.7063	0.6320	0.4792	0.6940	0.4710	0.5589	0.8269	0.9966	0.5626	0.5586	0.7031	0.6123	0.5212	0.7564	0.5163	0.5848	0.6747	0.5948	0.5381	0.6755	0.6940	0.5341	0.6556	0.5873	0.6872	0.6694	0.5366	0.6214	0.7128	0.5237	0.4485	0.4312	0.4755	0.5336	0.5792	0.7213
PCe 36:1	0.3314	0.3346	0.4195	0.5205	0.4230	0.3738	0.4097	0.4099	0.4442	0.3582	0.3199	0.4886	0.3691	0.4776	0.6601	0.7810	0.4515	0.3761	0.4631	0.4371	0.4109	0.5600	0.6434	0.4271	0.6037	0.5118	0.5987	0.4267	0.5547	0.5310	0.7320	0.5275	0.5287	0.5474	0.5286	0.5002	0.6885	0.5004	0.4802	0.5610	0.4714	0.7557	0.4803	0.4505
PCe 36:2	0.1375	0.1286	0.1276	0.1824	0.1536	0.1435	0.1650	0.1587	0.1793	0.1315	0.1068	0.1810	0.1388	0.1742	0.2680	0.3998	0.1575	0.1336	0.1555	0.1524	0.1634	0.1973	0.2626	0.1488	0.2531	0.1870	0.2434	0.1459	0.2191	0.2298	0.3717	0.2149	0.2046	0.2298	0.2342	0.1676	0.2976	0.2144	0.2246	0.2308	0.1816	0.4013	0.1900	0.1657
PCe 36:3	0.0826	0.0843	0.0857	0.1107	0.0962	0.0922	0.1238	0.1020	0.1289	0.0776	0.0698	0.1187	0.0870	0.1053	0.1736	0.1916	0.0944	0.0753	0.0957	0.0886	0.0870	0.1269	0.2204	0.0961	0.1985	0.1137	0.1640	0.1099	0.1688	0.1886	0.2907	0.1344	0.1395	0.1633	0.1640	0.1238	0.1628	0.1584	0.1649	0.1644	0.1091	0.2757	0.1531	0.1058
PCe 38:2	0.0499	0.0484	0.0459	0.0621	0.0576	0.0488	0.0590	0.0642	0.0745	0.0440	0.0356	0.0683	0.0506	0.0589	0.1086	0.2537	0.0591	0.0503	0.0519	0.0616	0.0613	0.0773	0.1548	0.0571	0.1263	0.0717	0.1038	0.0635	0.0983	0.1187	0.2508	0.0964	0.0871	0.1008	0.1156	0.0725	0.1478	0.1037	0.1080	0.1337	0.0656	0.2759	0.0888	0.0642
PCe 38:3	0.0503	0.0468	0.0534	0.0620	0.0613	0.0553	0.0660	0.0584	0.0687	0.0460	0.0392	0.0710	0.0592	0.0629	0.0924	0.1331	0.0629	0.0514	0.0608	0.0637	0.0597	0.0768	0.1034	0.0606	0.1039	0.0669	0.0874	0.0577	0.0825	0.0910	0.1610	0.0720	0.0686	0.0812	0.0937	0.0741	0.1028	0.0856	0.0780	0.0847	0.0593	0.1590	0.0691	0.0615
PCe 38:4	0.2544	0.2648	0.2703	0.3423	0.3075	0.2829	0.3138	0.2705	0.3052	0.2354	0.2251	0.3220	0.2910	0.3247	0.4105	0.3571	0.2968	0.2631	0.2830	0.3197	0.2918	0.3359	0.2354	0.2990	0.2365	0.3010	0.2674	0.2379	0.2666	0.2364	0.2732	0.2796	0.2875	0.3115	0.2943	0.3137	0.3232	0.2505	0.2619	0.1846	0.2684	0.2095	0.2589	0.2867
PCe 38:5	0.2003	0.2170	0.2386	0.2368	0.2448	0.2173	0.3807	0.2114	0.2430	0.2002	0.1922	0.2454	0.2434	0.2487	0.3707	0.2626	0.2564	0.2330	0.2411	0.2561	0.2394	0.2829	0.2217	0.2152	0.2086	0.2216	0.2136	0.1828	0.2281	0.1993	0.2556	0.2225	0.2303	0.2723	0.2536	0.2616	0.2387	0.1966	0.2022	0.1501	0.2180	0.1825	0.2235	0.2552
PCe 40:4	0.2050	0.1942	0.2066	0.1909	0.2018	0.2371	0.2461	0.1693	0.2373	0.1874	0.1994	0.2800	0.2431	0.2540	0.3581	0.2443	0.2439	0.2054	0.2051	0.2245	0.2386	0.2824	0.2820	0.2061	0.1845	0.3464	0.1993	0.2175	0.2354	0.2434	0.2440	0.3148	0.2394	0.2738	0.2756	0.3379	0.2431	0.2357	0.2252	0.2019	0.3183	0.1883	0.2584	0.3248
PCe 40:5	0.0963	0.1003	0.1134	0.1089	0.1042	0.0958	0.1418	0.0872	0.1080	0.0844	0.0853	0.1234	0.1026	0.1138	0.1686	0.1293	0.1152	0.0913	0.1113	0.1250	0.1197	0.1403	0.1123	0.1205	0.1097	0.1313	0.1020	0.0935	0.1185	0.1043	0.1251	0.1230	0.1144	0.1158	0.1332	0.1472	0.1316	0.0908	0.1088	0.0791	0.0997	0.1133	0.1058	0.1272
PCe 40:6	0.1851	0.1911	0.1812	0.2284	0.1795	0.1655	0.2448	0.1688	0.2167	0.1673	0.1520	0.2667	0.1748	0.2328	0.3495	0.2535	0.2165	0.1631	0.2098	0.2273	0.2035	0.2785	0.3387	0.2447	0.3279	0.3473	0.2834	0.2685	0.3209	0.2695	0.2887	0.4231	0.3100	0.3179	0.3039	0.3775	0.4348	0.2082	0.2315	0.2276	0.2812	0.3105	0.2177	0.3011
PCe 42:5	0.0383	0.0391	0.0404	0.0370	0.0367	0.0297	0.0525	0.0357	0.0383	0.0334	0.0387	0.0444	0.0353	0.0412	0.0763	0.0658	0.0451	0.0338	0.0453	0.0490	0.0459	0.0580	0.0553	0.0372	0.0567	0.0521	0.0453	0.0390	0.0583	0.0470	0.0510	0.0503	0.0465	0.0485	0.0604	0.0633	0.0554	0.0338	0.0444	0.0329	0.0527	0.0804	0.0584	0.0529
PCe 42:6	0.0367	0.0364	0.0375	0.0423	0.0340	0.0327	0.0506	0.0344	0.0462	0.0378	0.0316	0.0519	0.0405	0.0457	0.0747	0.0648	0.0437	0.0322	0.0440	0.0469	0.0478	0.0596	0.0566	0.0464	0.0575	0.0629	0.0437	0.0472	0.0614	0.0573	0.0612	0.0623	0.0501	0.0510	0.0679	0.0641	0.0646	0.0350	0.0438	0.0396	0.0435	0.0880	0.0516	0.0553
LPC 16:0	0.4101	0.4910	0.5509	0.4776	0.5453	0.5270	0.6110	0.4613	0.5363	0.6225	0.5199	0.6374	0.6914	0.4830	0.5445	0.7188	0.5054	0.5719	0.5378	0.8053	0.6855	0.5918	0.5579	0.5596	0.4779	0.5111	0.3825	0.4586	0.4934	0.4577	0.6694	0.4560	0.4820	0.4995	0.6229	0.5462	0.4300	0.2754	0.4959	0.5084	0.4407	0.7003	0.6105	0.4309
LPC 18:0	0.1764	0.1952	0.2040	0.2100	0.2001	0.2050	0.2687	0.1988	0.2213	0.2283	0.2288	0.2742	0.2815	0.1939	0.2069	0.2999	0.1991	0.2405	0.2156	0.2859	0.2871	0.2545	0.2666	0.2074	0.2210	0.2023	0.1594	0.1681	0.2006	0.2081	0.3801	0.1777	0.1870	0.1935	0.2637	0.2110	0.1848	0.1127	0.2049	0.2182	0.1542	0.3292	0.2345	0.1653
LPC 18:1	0.1567	0.1611	0.1701	0.1602	0.1773	0.1737	0.2278	0.1615	0.1998	0.2165	0.1949	0.2545	0.2357	0.1766	0.1854	0.2713	0.1773	0.1936	0.1978	0.2831	0.2441	0.2284	0.2567	0.1698	0.2408	0.2121	0.1665	0.1693	0.1981	0.2081	0.3874	0.1786	0.1988	0.1825	0.2301	0.1846	0.1642	0.1153	0.2024	0.2385	0.1379	0.3880	0.2398	0.1572
LPC 20:3	0.0036	0.0036	0.0041	0.0034	0.0040	0.0044	0.0073	0.0042	0.0044	0.0049	0.0056	0.0067	0.0067	0.0040	0.0041	0.0052	0.0043	0.0053	0.0052	0.0077	0.0063	0.0055	0.0049	0.0041	0.0039	0.0041	0.0031	0.0036	0.0045	0.0041	0.0060	0.0042	0.0043	0.0040	0.0044	0.0037	0.0031	0.0019	0.0037	0.0033	0.0026	0.0049	0.0048	0.0034
LPC 20:4	0.0346	0.0322	0.0351	0.0299	0.0378	0.0454	0.0591	0.0391	0.0391	0.0485	0.0609	0.0687	0.0637	0.0394	0.0398	0.0434	0.0377	0.0470	0.0458	0.0763	0.0590	0.0517	0.0390	0.0455	0.0367	0.0354	0.0257	0.0325	0.0373	0.0327	0.0492	0.0366	0.0378	0.0327	0.0362	0.0336	0.0255	0.0136	0.0332	0.0245	0.0240	0.0327	0.0450	0.0329
LPCe 16:0	0.0008	0.0009	0.0011	0.0014	0.0011	0.0011	0.0016	0.0009	0.0009	0.0013	0.0010	0.0015	0.0016	0.0012	0.0015	0.0016	0.0011	0.0012	0.0012	0.0015	0.0014	0.0013	0.0025	0.0024	0.0014	0.0016	0.0014	0.0020	0.0016	0.0012	0.0020	0.0016	0.0015	0.0022	0.0021	0.0020	0.0020	0.0009	0.0011	0.0013	0.0012	0.0019	0.0013	0.0011
LPCe 18:0	0.1699	0.1652	0.1688	0.1649	0.1657	0.1657	0.1692	0.1657	0.1686	0.1682	0.1651	0.1679	0.1690	0.1694	0.1649	0.1651	0.1653	0.1657	0.1651	0.1647	0.1679	0.1650	0.1716	0.1644	0.1657	0.1644	0.1702	0.1657	0.1709	0.1649	0.1727	0.1676	0.1704	0.1698	0.1691	0.1645	0.1697	0.1647	0.1651	0.1691	0.1689	0.1656	0.1696	0.1681
LPCe 18:1	0.0024	0.0031	0.0025	0.0030	0.0034	0.0031	0.0031	0.0031	0.0027	0.0034	0.0027	0.0032	0.0036	0.0026	0.0029	0.0038	0.0029	0.0025	0.0026	0.0037	0.0032	0.0032	0.0042	0.0036	0.0034	0.0035	0.0028	0.0033	0.0032	0.0034	0.0054	0.0031	0.0039	0.0040	0.0047	0.0034	0.0035	0.0028	0.0034	0.0037	0.0033	0.0064	0.0043	0.0033
LPCe 20:0	0.0006	0.0007	0.0007	0.0006	0.0006	0.0007	0.0013	0.0009	0.0007	0.0008	0.0006	0.0008	0.0012	0.0007	0.0010	0.0015	0.0007	0.0008	0.0006	0.0010	0.0010	0.0009	0.0016	0.0009	0.0011	0.0009	0.0009	0.0009	0.0009	0.0012	0.0026	0.0009	0.0010	0.0010	0.0015	0.0008	0.0010	0.0006	0.0009	0.0012	0.0006	0.0017	0.0009	0.0007
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
Cer 16:0
Cer 16:1
Cer 18:0
Cer 18:1
Cer 20:0
Cer 20:1
Cer 22:0
Cer 22:1
Cer 24:0
Cer 24:1
SM 16:0
SM 18:0
SM 18:1
SM 20:0
SM 22:0
SM 24:0
SM 24:1
SM 26:0
SM 26:1
dhSM 16:0
dhSM 16:1
dhSM 18:0
dhSM 18:1
dhSM 20:0
dhSM 20:1
dhSM 22:0
dhSM 22:1
dhSM 24:0
dhSM 24:1
dhSM 26:1
dhSM 26:2
HexCer 16:0
HexCer 16:1
HexCer 18:0
HexCer 18:1
HexCer 20:0
HexCer 20:1
HexCer 22:0
HexCer 22:1
HexCer 24:0
HexCer 24:1
HexCer 26:0
HexCer 26:1
LacCer 16:0
LacCer 18:0
LacCer 20:0
LacCer 22:0
LacCer 24:0
LacCer 24:1
PC 30:0
PC 32:0
PC 32:1
PC 34:0
PC 34:1
PC 34:2
PC 36:0
PC 36:1
PC 36:2
PC 36:3
PC 36:4
PC 38:0
PC 38:1
PC 38:2
PC 38:3
PC 38:4
PC 38:5
PC 38:6
PC 40:4
PC 40:5
PC 40:6
PC 40:7
PC 42:5
PC 42:6
PC 42:7
PCe 30:0
PCe 32:0
PCe 32:1
PCe 34:0
PCe 34:1
PCe 34:2
PCe 36:0
PCe 36:1
PCe 36:2
PCe 36:3
PCe 38:2
PCe 38:3
PCe 38:4
PCe 38:5
PCe 40:4
PCe 40:5
PCe 40:6
PCe 42:5
PCe 42:6
LPC 16:0
LPC 18:0
LPC 18:1
LPC 20:3
LPC 20:4
LPCe 16:0
LPCe 18:0
LPCe 18:1
LPCe 20:0
METABOLITES_END
#END