#METABOLOMICS WORKBENCH sven_haange_20210615_230533_mwtab.txt DATATRACK_ID:2693 STUDY_ID:ST001832 ANALYSIS_ID:AN002974 PROJECT_ID:PR001157
VERSION             	1
CREATED_ON             	June 17, 2021, 5:53 pm
#PROJECT
PR:PROJECT_TITLE                 	Fecal Transplant from Roux-Y-Gastric-Bypass rat model into a diet induced
PR:PROJECT_TITLE                 	obesity rat model
PR:PROJECT_SUMMARY               	Investigating alterations in the intestinal microbiome metabolome and host blood
PR:PROJECT_SUMMARY               	metabolome in a diet induced obesity (DIO) rat model after fecal transplant from
PR:PROJECT_SUMMARY               	rats, which underwent Roux-Y-Gastric-Bypass surgery (RYGB). Experimental groups
PR:PROJECT_SUMMARY               	for rat experiments. 1)Rats in the ‘RYGB_Donor group’ underwent RYGB
PR:PROJECT_SUMMARY               	surgery. Postoperatively, animals received a two-choice diet, consisting of a SC
PR:PROJECT_SUMMARY               	and HFD. Once the animals recovered and achieved a stabilized weight reduction
PR:PROJECT_SUMMARY               	at 4 weeks postoperatively, body weight and food intake were recorded daily.
PR:PROJECT_SUMMARY               	2)Rats allocated into the ‘RYGB_AB group’ were handled the same as animals
PR:PROJECT_SUMMARY               	in the ‘RYGB_donor group’. From postoperative week 4 onwards, animals
PR:PROJECT_SUMMARY               	received an antibiotic treatment (AB) consisting of ampicillin (1 g/l;
PR:PROJECT_SUMMARY               	Ratiopharm), vancomycin (0.5 g/l; Ratiopharm), neomycin (1 g/l; Bela-pharm), and
PR:PROJECT_SUMMARY               	metronidazole (1 g/l; CP-Pharma), provided freshly every day via drinking
PR:PROJECT_SUMMARY               	water.[19, 20] All antibiotics were given for the time period indicated in each
PR:PROJECT_SUMMARY               	figure.[21] 3)Rats in the ‘Lean group’ served as healthy, age-matched
PR:PROJECT_SUMMARY               	controls, constantly kept under SC. 4)Rats in the ‘Lean_AB group’ received
PR:PROJECT_SUMMARY               	the same antibiotic treatment as animals in the RYGB_AB group, serving as a
PR:PROJECT_SUMMARY               	control for antibiotic-specific side effects. All antibiotics were given for the
PR:PROJECT_SUMMARY               	time periods indicated in each figure. 5)Rats in the ‘DIO group’ received no
PR:PROJECT_SUMMARY               	surgery, but were otherwise handled like RYGB litter mates. 6)Rats in the
PR:PROJECT_SUMMARY               	‘DIO_FMT_RYGB group’ received fecal RYGB_Donor microbiota transplantation
PR:PROJECT_SUMMARY               	once per week, but were otherwise handled like DIO litter mates. For fecal
PR:PROJECT_SUMMARY               	transplantation experiments, 100 mg of fresh stool from body weight-stabilized
PR:PROJECT_SUMMARY               	RYGB donors were re-suspended in 1 ml of PBS, homogenized carefully and
PR:PROJECT_SUMMARY               	administered via oral gavage with 200 μl of the suspension. 7)Rats in the
PR:PROJECT_SUMMARY               	‘FMT_Lean group’ were handled like DIO litter mates, but were orally gavaged
PR:PROJECT_SUMMARY               	with 200 μl of filtrate received from lean donors, and served as a control for
PR:PROJECT_SUMMARY               	fecal transplantation and specificity of effect size(s) as a function of the
PR:PROJECT_SUMMARY               	donor microbiota. 8)Rats in the ‘DIO_PF_FMT group’ were handled like DIO
PR:PROJECT_SUMMARY               	litter mates, but pair-fed to the SC/HFD food intake of ‘FMT_Lean littermates,
PR:PROJECT_SUMMARY               	serving as a control for effects secondary to reduced caloric intake.
PR:INSTITUTE                     	Helmholtz Centre for Environmental Research - UFZ
PR:LAST_NAME                     	Haange
PR:FIRST_NAME                    	Sven
PR:ADDRESS                       	Permoserstrasse 1, Leipzig, Saxony, 04318, Germany
PR:EMAIL                         	sven.haange@ufz.de
PR:PHONE                         	0049 341 2351099
#STUDY
ST:STUDY_TITLE                   	Quantitative bile acids study on blood serum and ceacal content of rat models
ST:STUDY_TITLE                   	(part I)
ST:STUDY_SUMMARY                 	Bile acids were analyzed in blood serum and cecal content in rat models
ST:INSTITUTE                     	Helmholtz Centre for Environmental Research - UFZ
ST:DEPARTMENT                    	Department of Molecular Systems biology
ST:LABORATORY                    	Functional Metabolomics
ST:LAST_NAME                     	Rolle-Kampczyk
ST:FIRST_NAME                    	Ulrike
ST:ADDRESS                       	Permoserstrasse 15, 04318 Leipzig, Germany
ST:EMAIL                         	ulrike.rolle-kampczyk@ufz.de
ST:STUDY_TYPE                    	blood serum and caecum content
ST:PHONE                         	0049 341 235 1537
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Rattus norvegicus
SU:TAXONOMY_ID                   	10116
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	1024040931	Tissue:Caecum | Rat_number:1 | Rat_sample_name:DIO_1 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5502_1024038647_56_0_1_1_00_1024040931.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024039050	Tissue:Caecum | Rat_number:129 | Rat_sample_name:DIO_10 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5512_1024036014_18_0_1_1_00_1024039050.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041499	Tissue:Caecum | Rat_number:67 | Rat_sample_name:DIO_11 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5512_1024036014_51_0_1_1_00_1024041499.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024040999	Tissue:Caecum | Rat_number:7 | Rat_sample_name:DIO_2 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5502_1024038647_33_0_1_1_00_1024040999.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041015	Tissue:Caecum | Rat_number:9 | Rat_sample_name:DIO_3 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5502_1024038647_57_0_1_1_00_1024041015.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041020	Tissue:Caecum | Rat_number:10 | Rat_sample_name:DIO_4 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5502_1024038647_69_0_1_1_00_1024041020.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024039045	Tissue:Caecum | Rat_number:128 | Rat_sample_name:DIO_7 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5512_1024036014_06_0_1_1_00_1024039045.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041673	Tissue:Caecum | Rat_number:126 | Rat_sample_name:DIO_8 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5512_1024036014_77_0_1_1_00_1024041673.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024039031	Tissue:Caecum | Rat_number:127 | Rat_sample_name:DIO_9 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5512_1024036014_89_0_1_1_00_1024039031.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024040946	Tissue:Caecum | Rat_number:2 | Rat_sample_name:DIO_AB_1 | Sample treatment:DIO_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_68_0_1_1_00_1024040946.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024040951	Tissue:Caecum | Rat_number:3 | Rat_sample_name:DIO_AB_2 | Sample treatment:DIO_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_80_0_1_1_00_1024040951.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024040965	Tissue:Caecum | Rat_number:4 | Rat_sample_name:DIO_AB_3 | Sample treatment:DIO_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_92_0_1_1_00_1024040965.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024040984	Tissue:Caecum | Rat_number:6 | Rat_sample_name:DIO_AB_4 | Sample treatment:DIO_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_21_0_1_1_00_1024040984.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035907	Tissue:Caecum | Rat_number:49 | Rat_sample_name:DIO_AB_5 | Sample treatment:DIO_AB	RAW_FILE_NAME=BA02-0-5512_1024036014_62_0_1_1_00_1024035907.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038632	Tissue:Caecum | Rat_number:47 | Rat_sample_name:DIO_AB_7 | Sample treatment:DIO_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_96_0_1_1_00_1024038632.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024040970	Tissue:Caecum | Rat_number:5 | Rat_sample_name:DIO_FMT_RYGB_1 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5502_1024038647_09_0_1_1_00_1024040970.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041635	Tissue:Caecum | Rat_number:122 | Rat_sample_name:DIO_FMT_RYGB_10 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5512_1024036014_29_0_1_1_00_1024041635.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041669	Tissue:Caecum | Rat_number:131 | Rat_sample_name:DIO_FMT_RYGB_12 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5512_1024036014_65_0_1_1_00_1024041669.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041654	Tissue:Caecum | Rat_number:130 | Rat_sample_name:DIO_FMT_RYGB_11 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5512_1024036014_53_0_1_1_00_1024041654.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041001	Tissue:Caecum | Rat_number:8 | Rat_sample_name:DIO_FMT_RYGB_2 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5502_1024038647_45_0_1_1_00_1024041001.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041034	Tissue:Caecum | Rat_number:11 | Rat_sample_name:DIO_FMT_RYGB_3 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5502_1024038647_81_0_1_1_00_1024041034.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041049	Tissue:Caecum | Rat_number:12 | Rat_sample_name:DIO_FMT_RYGB_4 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5502_1024038647_93_0_1_1_00_1024041049.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041514	Tissue:Caecum | Rat_number:72 | Rat_sample_name:DIO_FMT_RYGB_7 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5512_1024036014_75_0_1_1_00_1024041514.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035931	Tissue:Caecum | Rat_number:57 | Rat_sample_name:DIO_FMT_RYGB_8 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5512_1024036014_03_0_1_1_00_1024035931.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041640	Tissue:Caecum | Rat_number:123 | Rat_sample_name:DIO_FMT_RYGB_9 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5512_1024036014_41_0_1_1_00_1024041640.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024039083	Tissue:Caecum | Rat_number:132 | Rat_sample_name:DIO_PF_FMT_1 | Sample treatment:DIO_PF_FMT	RAW_FILE_NAME=BA02-0-5512_1024036014_54_0_1_1_00_1024039083.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024039109	Tissue:Caecum | Rat_number:134 | Rat_sample_name:DIO_PF_FMT_2 | Sample treatment:DIO_PF_FMT	RAW_FILE_NAME=BA02-0-5512_1024036014_78_0_1_1_00_1024039109.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024039113	Tissue:Caecum | Rat_number:135 | Rat_sample_name:DIO_PF_FMT_3 | Sample treatment:DIO_PF_FMT	RAW_FILE_NAME=BA02-0-5512_1024036014_90_0_1_1_00_1024039113.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024039098	Tissue:Caecum | Rat_number:133 | Rat_sample_name:DIO_PF_FMT_4 | Sample treatment:DIO_PF_FMT	RAW_FILE_NAME=BA02-0-5512_1024036014_66_0_1_1_00_1024039098.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024039079	Tissue:Caecum | Rat_number:125 | Rat_sample_name:DIO_PF_FMT_6 | Sample treatment:DIO_PF_FMT	RAW_FILE_NAME=BA02-0-5512_1024036014_42_0_1_1_00_1024039079.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035896	Tissue:Caecum | Rat_number:48 | Rat_sample_name:FMT_lean_1 | Sample treatment:FMT_lean	RAW_FILE_NAME=BA02-0-5512_1024036014_50_0_1_1_00_1024035896.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035945	Tissue:Caecum | Rat_number:58 | Rat_sample_name:FMT_lean_2 | Sample treatment:FMT_lean	RAW_FILE_NAME=BA02-0-5512_1024036014_15_0_1_1_00_1024035945.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035964	Tissue:Caecum | Rat_number:64 | Rat_sample_name:FMT_lean_4 | Sample treatment:FMT_lean	RAW_FILE_NAME=BA02-0-5512_1024036014_39_0_1_1_00_1024035964.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041529	Tissue:Caecum | Rat_number:73 | Rat_sample_name:FMT_lean_5 | Sample treatment:FMT_lean	RAW_FILE_NAME=BA02-0-5512_1024036014_87_0_1_1_00_1024041529.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041533	Tissue:Caecum | Rat_number:74 | Rat_sample_name:FMT_lean_6 | Sample treatment:FMT_lean	RAW_FILE_NAME=BA02-0-5512_1024036014_04_0_1_1_00_1024041533.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038481	Tissue:Caecum | Rat_number:29 | Rat_sample_name:Lean_1 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5502_1024038647_11_0_1_1_00_1024038481.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024039151	Tissue:Caecum | Rat_number:139 | Rat_sample_name:Lean_10 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5512_1024036014_43_0_1_1_00_1024039151.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038496	Tissue:Caecum | Rat_number:30 | Rat_sample_name:Lean_2 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5502_1024038647_23_0_1_1_00_1024038496.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038526	Tissue:Caecum | Rat_number:33 | Rat_sample_name:Lean_3 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5502_1024038647_59_0_1_1_00_1024038526.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038531	Tissue:Caecum | Rat_number:34 | Rat_sample_name:Lean_4 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5502_1024038647_71_0_1_1_00_1024038531.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035926	Tissue:Caecum | Rat_number:55 | Rat_sample_name:Lean_6 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5512_1024036014_86_0_1_1_00_1024035926.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024039128	Tissue:Caecum | Rat_number:99 | Rat_sample_name:Lean_7 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5512_1024036014_07_0_1_1_00_1024039128.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024039132	Tissue:Caecum | Rat_number:100 | Rat_sample_name:Lean_8 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5512_1024036014_19_0_1_1_00_1024039132.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024039147	Tissue:Caecum | Rat_number:138 | Rat_sample_name:Lean_9 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5512_1024036014_31_0_1_1_00_1024039147.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038462	Tissue:Caecum | Rat_number:27 | Rat_sample_name:Lean_AB_1 | Sample treatment:Lean_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_82_0_1_1_00_1024038462.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038477	Tissue:Caecum | Rat_number:28 | Rat_sample_name:Lean_AB_2 | Sample treatment:Lean_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_94_0_1_1_00_1024038477.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038507	Tissue:Caecum | Rat_number:31 | Rat_sample_name:Lean_AB_3 | Sample treatment:Lean_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_35_0_1_1_00_1024038507.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038511	Tissue:Caecum | Rat_number:32 | Rat_sample_name:Lean_AB_4 | Sample treatment:Lean_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_47_0_1_1_00_1024038511.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038613	Tissue:Caecum | Rat_number:42 | Rat_sample_name:Lean_AB_5 | Sample treatment:Lean_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_72_0_1_1_00_1024038613.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038628	Tissue:Caecum | Rat_number:43 | Rat_sample_name:Lean_AB_6 | Sample treatment:Lean_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_84_0_1_1_00_1024038628.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041072	Tissue:Caecum | Rat_number:15a | Rat_sample_name:RYGB_AB_1 | Sample treatment:RYGB_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_34_0_1_1_00_1024041072.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041053	Tissue:Caecum | Rat_number:13 | Rat_sample_name:RYGB_AB_2 | Sample treatment:RYGB_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_10_0_1_1_00_1024041053.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041102	Tissue:Caecum | Rat_number:23 | Rat_sample_name:RYGB_AB_3 | Sample treatment:RYGB_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_70_0_1_1_00_1024041102.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038579	Tissue:Caecum | Rat_number:39 | Rat_sample_name:RYGB_AB_4 | Sample treatment:RYGB_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_24_0_1_1_00_1024038579.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038583	Tissue:Caecum | Rat_number:40 | Rat_sample_name:RYGB_AB_5 | Sample treatment:RYGB_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_36_0_1_1_00_1024038583.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038609	Tissue:Caecum | Rat_number:41 | Rat_sample_name:RYGB_AB_6 | Sample treatment:RYGB_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_60_0_1_1_00_1024038609.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041591	Tissue:Caecum | Rat_number:117 | Rat_sample_name:RYGB_AB_7 | Sample treatment:RYGB_AB	RAW_FILE_NAME=BA02-0-5512_1024036014_76_0_1_1_00_1024041591.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041621	Tissue:Caecum | Rat_number:119 | Rat_sample_name:RYGB_AB_8 | Sample treatment:RYGB_AB	RAW_FILE_NAME=BA02-0-5512_1024036014_17_0_1_1_00_1024041621.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041586	Tissue:Caecum | Rat_number:111 | Rat_sample_name:RYGB_AB_9 | Sample treatment:RYGB_AB	RAW_FILE_NAME=BA02-0-5512_1024036014_64_0_1_1_00_1024041586.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041571	Tissue:Caecum | Rat_number:114 | Rat_sample_name:RYGB_Donor_10 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5512_1024036014_52_0_1_1_00_1024041571.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041087	Tissue:Caecum | Rat_number:17 | Rat_sample_name:RYGB_Donor_2 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5502_1024038647_46_0_1_1_00_1024041087.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041091	Tissue:Caecum | Rat_number:20 | Rat_sample_name:RYGB_Donor_3 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5502_1024038647_58_0_1_1_00_1024041091.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038545	Tissue:Caecum | Rat_number:35 | Rat_sample_name:RYGB_Donor_4 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5502_1024038647_83_0_1_1_00_1024038545.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038564	Tissue:Caecum | Rat_number:37 | Rat_sample_name:RYGB_Donor_5 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5502_1024038647_12_0_1_1_00_1024038564.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038550	Tissue:Caecum | Rat_number:36 | Rat_sample_name:RYGB_Donor_6 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5502_1024038647_95_0_1_1_00_1024038550.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041552	Tissue:Caecum | Rat_number:107 | Rat_sample_name:RYGB_Donor_7 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5512_1024036014_28_0_1_1_00_1024041552.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041567	Tissue:Caecum | Rat_number:109 | Rat_sample_name:RYGB_Donor_8 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5512_1024036014_40_0_1_1_00_1024041567.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024041548	Tissue:Caecum | Rat_number:106 | Rat_sample_name:RYGB_Donor_9 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5512_1024036014_16_0_1_1_00_1024041548.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038375	Tissue:Serum | Rat_number:1 | Rat_sample_name:DIO_1 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5502_1024038647_50_0_1_1_00_1024038375.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024033225	Tissue:Serum | Rat_number:129 | Rat_sample_name:DIO_10 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5512_1024036014_57_0_1_1_00_1024033225.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035363	Tissue:Serum | Rat_number:67 | Rat_sample_name:DIO_11 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5502_1024038647_91_0_1_1_00_1024035363.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035378	Tissue:Serum | Rat_number:70 | Rat_sample_name:DIO_12 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5502_1024038647_08_0_1_1_00_1024035378.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038443	Tissue:Serum | Rat_number:9 | Rat_sample_name:DIO_3 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5502_1024038647_39_0_1_1_00_1024038443.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038458	Tissue:Serum | Rat_number:10 | Rat_sample_name:DIO_4 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5502_1024038647_51_0_1_1_00_1024038458.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024033211	Tissue:Serum | Rat_number:128 | Rat_sample_name:DIO_7 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5512_1024036014_45_0_1_1_00_1024033211.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024033195	Tissue:Serum | Rat_number:126 | Rat_sample_name:DIO_8 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5512_1024036014_21_0_1_1_00_1024033195.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024033206	Tissue:Serum | Rat_number:127 | Rat_sample_name:DIO_9 | Sample treatment:DIO	RAW_FILE_NAME=BA02-0-5512_1024036014_33_0_1_1_00_1024033206.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038380	Tissue:Serum | Rat_number:2 | Rat_sample_name:DIO_AB_1 | Sample treatment:DIO_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_62_0_1_1_00_1024038380.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038394	Tissue:Serum | Rat_number:3 | Rat_sample_name:DIO_AB_2 | Sample treatment:DIO_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_74_0_1_1_00_1024038394.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038405	Tissue:Serum | Rat_number:4 | Rat_sample_name:DIO_AB_3 | Sample treatment:DIO_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_86_0_1_1_00_1024038405.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038424	Tissue:Serum | Rat_number:6 | Rat_sample_name:DIO_AB_4 | Sample treatment:DIO_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_15_0_1_1_00_1024038424.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035281	Tissue:Serum | Rat_number:49 | Rat_sample_name:DIO_AB_5 | Sample treatment:DIO_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_90_0_1_1_00_1024035281.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035261	Tissue:Serum | Rat_number:47 | Rat_sample_name:DIO_AB_7 | Sample treatment:DIO_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_66_0_1_1_00_1024035261.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038410	Tissue:Serum | Rat_number:5 | Rat_sample_name:DIO_FMT_RYGB_1 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5502_1024038647_03_0_1_1_00_1024038410.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024033157	Tissue:Serum | Rat_number:122 | Rat_sample_name:DIO_FMT_RYGB_10 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5512_1024036014_68_0_1_1_00_1024033157.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024038439	Tissue:Serum | Rat_number:8 | Rat_sample_name:DIO_FMT_RYGB_2 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5502_1024038647_27_0_1_1_00_1024038439.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032366	Tissue:Serum | Rat_number:11 | Rat_sample_name:DIO_FMT_RYGB_3 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5502_1024038647_63_0_1_1_00_1024032366.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032371	Tissue:Serum | Rat_number:12 | Rat_sample_name:DIO_FMT_RYGB_4 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5502_1024038647_75_0_1_1_00_1024032371.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035382	Tissue:Serum | Rat_number:72 | Rat_sample_name:DIO_FMT_RYGB_7 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5502_1024038647_20_0_1_1_00_1024035382.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035311	Tissue:Serum | Rat_number:57 | Rat_sample_name:DIO_FMT_RYGB_8 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5502_1024038647_31_0_1_1_00_1024035311.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024033161	Tissue:Serum | Rat_number:123 | Rat_sample_name:DIO_FMT_RYGB_9 | Sample treatment:DIO_FMT_RYGB	RAW_FILE_NAME=BA02-0-5512_1024036014_80_0_1_1_00_1024033161.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024033259	Tissue:Serum | Rat_number:132 | Rat_sample_name:DIO_PF_FMT_1 | Sample treatment:DIO_PF_FMT	RAW_FILE_NAME=BA02-0-5512_1024036014_93_0_1_1_00_1024033259.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024033282	Tissue:Serum | Rat_number:134 | Rat_sample_name:DIO_PF_FMT_2 | Sample treatment:DIO_PF_FMT	RAW_FILE_NAME=BA02-0-5512_1024036014_34_0_1_1_00_1024033282.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024033230	Tissue:Serum | Rat_number:124 | Rat_sample_name:DIO_PF_FMT_5 | Sample treatment:DIO_PF_FMT	RAW_FILE_NAME=BA02-0-5512_1024036014_69_0_1_1_00_1024033230.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024033244	Tissue:Serum | Rat_number:125 | Rat_sample_name:DIO_PF_FMT_6 | Sample treatment:DIO_PF_FMT	RAW_FILE_NAME=BA02-0-5512_1024036014_81_0_1_1_00_1024033244.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035276	Tissue:Serum | Rat_number:48 | Rat_sample_name:FMT_lean_1 | Sample treatment:FMT_lean	RAW_FILE_NAME=BA02-0-5502_1024038647_78_0_1_1_00_1024035276.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035325	Tissue:Serum | Rat_number:58 | Rat_sample_name:FMT_lean_2 | Sample treatment:FMT_lean	RAW_FILE_NAME=BA02-0-5502_1024038647_43_0_1_1_00_1024035325.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035330	Tissue:Serum | Rat_number:63 | Rat_sample_name:FMT_lean_3 | Sample treatment:FMT_lean	RAW_FILE_NAME=BA02-0-5502_1024038647_55_0_1_1_00_1024035330.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035359	Tissue:Serum | Rat_number:64 | Rat_sample_name:FMT_lean_4 | Sample treatment:FMT_lean	RAW_FILE_NAME=BA02-0-5502_1024038647_79_0_1_1_00_1024035359.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035397	Tissue:Serum | Rat_number:73 | Rat_sample_name:FMT_lean_5 | Sample treatment:FMT_lean	RAW_FILE_NAME=BA02-0-5502_1024038647_32_0_1_1_00_1024035397.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024040927	Tissue:Serum | Rat_number:74 | Rat_sample_name:FMT_lean_6 | Sample treatment:FMT_lean	RAW_FILE_NAME=BA02-0-5502_1024038647_44_0_1_1_00_1024040927.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032453	Tissue:Serum | Rat_number:29 | Rat_sample_name:Lean_1 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5502_1024038647_76_0_1_1_00_1024032453.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035998	Tissue:Serum | Rat_number:139 | Rat_sample_name:Lean_10 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5512_1024036014_94_0_1_1_00_1024035998.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032468	Tissue:Serum | Rat_number:30 | Rat_sample_name:Lean_2 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5502_1024038647_88_0_1_1_00_1024032468.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032502	Tissue:Serum | Rat_number:33 | Rat_sample_name:Lean_3 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5502_1024038647_41_0_1_1_00_1024032502.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032517	Tissue:Serum | Rat_number:34 | Rat_sample_name:Lean_4 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5502_1024038647_53_0_1_1_00_1024032517.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035295	Tissue:Serum | Rat_number:54 | Rat_sample_name:Lean_5 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5502_1024038647_07_0_1_1_00_1024035295.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035306	Tissue:Serum | Rat_number:55 | Rat_sample_name:Lean_6 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5502_1024038647_19_0_1_1_00_1024035306.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024033308	Tissue:Serum | Rat_number:99 | Rat_sample_name:Lean_7 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5512_1024036014_58_0_1_1_00_1024033308.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035979	Tissue:Serum | Rat_number:100 | Rat_sample_name:Lean_8 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5512_1024036014_70_0_1_1_00_1024035979.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035983	Tissue:Serum | Rat_number:138 | Rat_sample_name:Lean_9 | Sample treatment:Lean	RAW_FILE_NAME=BA02-0-5512_1024036014_82_0_1_1_00_1024035983.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032449	Tissue:Serum | Rat_number:28 | Rat_sample_name:Lean_AB_2 | Sample treatment:Lean_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_64_0_1_1_00_1024032449.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032487	Tissue:Serum | Rat_number:31 | Rat_sample_name:Lean_AB_3 | Sample treatment:Lean_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_17_0_1_1_00_1024032487.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032491	Tissue:Serum | Rat_number:32 | Rat_sample_name:Lean_AB_4 | Sample treatment:Lean_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_29_0_1_1_00_1024032491.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035242	Tissue:Serum | Rat_number:42 | Rat_sample_name:Lean_AB_5 | Sample treatment:Lean_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_42_0_1_1_00_1024035242.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035257	Tissue:Serum | Rat_number:43 | Rat_sample_name:Lean_AB_6 | Sample treatment:Lean_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_54_0_1_1_00_1024035257.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032390	Tissue:Serum | Rat_number:15a | Rat_sample_name:RYGB_AB_1 | Sample treatment:RYGB_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_04_0_1_1_00_1024032390.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032385	Tissue:Serum | Rat_number:13 | Rat_sample_name:RYGB_AB_2 | Sample treatment:RYGB_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_87_0_1_1_00_1024032385.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032434	Tissue:Serum | Rat_number:23 | Rat_sample_name:RYGB_AB_3 | Sample treatment:RYGB_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_52_0_1_1_00_1024032434.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035219	Tissue:Serum | Rat_number:39 | Rat_sample_name:RYGB_AB_4 | Sample treatment:RYGB_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_06_0_1_1_00_1024035219.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035223	Tissue:Serum | Rat_number:40 | Rat_sample_name:RYGB_AB_5 | Sample treatment:RYGB_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_18_0_1_1_00_1024035223.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024035238	Tissue:Serum | Rat_number:41 | Rat_sample_name:RYGB_AB_6 | Sample treatment:RYGB_AB	RAW_FILE_NAME=BA02-0-5502_1024038647_30_0_1_1_00_1024035238.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024033123	Tissue:Serum | Rat_number:117 | Rat_sample_name:RYGB_AB_7 | Sample treatment:RYGB_AB	RAW_FILE_NAME=BA02-0-5512_1024036014_32_0_1_1_00_1024033123.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024033142	Tissue:Serum | Rat_number:119 | Rat_sample_name:RYGB_AB_8 | Sample treatment:RYGB_AB	RAW_FILE_NAME=BA02-0-5512_1024036014_56_0_1_1_00_1024033142.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032415	Tissue:Serum | Rat_number:18b | Rat_sample_name:RYGB_Donor_1 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5502_1024038647_28_0_1_1_00_1024032415.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024039201	Tissue:Serum | Rat_number:114 | Rat_sample_name:RYGB_Donor_10 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5512_1024036014_08_0_1_1_00_1024039201.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032401	Tissue:Serum | Rat_number:17 | Rat_sample_name:RYGB_Donor_2 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5502_1024038647_16_0_1_1_00_1024032401.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032420	Tissue:Serum | Rat_number:20 | Rat_sample_name:RYGB_Donor_3 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5502_1024038647_40_0_1_1_00_1024032420.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032521	Tissue:Serum | Rat_number:35 | Rat_sample_name:RYGB_Donor_4 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5502_1024038647_65_0_1_1_00_1024032521.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032541	Tissue:Serum | Rat_number:37 | Rat_sample_name:RYGB_Donor_5 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5502_1024038647_89_0_1_1_00_1024032541.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024032536	Tissue:Serum | Rat_number:36 | Rat_sample_name:RYGB_Donor_6 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5502_1024038647_77_0_1_1_00_1024032536.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024039185	Tissue:Serum | Rat_number:107 | Rat_sample_name:RYGB_Donor_7 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5512_1024036014_79_0_1_1_00_1024039185.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024039190	Tissue:Serum | Rat_number:109 | Rat_sample_name:RYGB_Donor_8 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5512_1024036014_91_0_1_1_00_1024039190.wiff
SUBJECT_SAMPLE_FACTORS           	-	1024039166	Tissue:Serum | Rat_number:106 | Rat_sample_name:RYGB_Donor_9 | Sample treatment:RYGB_Donor	RAW_FILE_NAME=BA02-0-5512_1024036014_55_0_1_1_00_1024039166.wiff
#COLLECTION
CO:COLLECTION_SUMMARY            	Animals were sacrificed, blood and cecum tissue were removed, snap frozen in
CO:COLLECTION_SUMMARY            	liquid nitrogen and stored at -80°C until further analysis
CO:SAMPLE_TYPE                   	Cecum
#TREATMENT
TR:TREATMENT_SUMMARY             	RYGB surgery was performed on age-matched (8-10-weeks old) male Wistar IGS rats.
TR:TREATMENT_SUMMARY             	After four weeks of recovery, animals were put on an antibiotic cocktail
TR:TREATMENT_SUMMARY             	consisting of ampicillin (1 g/l; Ratiopharm), vancomycin (0.5 g/l; Ratiopharm),
TR:TREATMENT_SUMMARY             	neomycin (1 g/l; Bela-pharm), and metronidazole (1 g/l; CP-Pharma), provided
TR:TREATMENT_SUMMARY             	freshly every day via drinking water. The parallel FMT group received fecal RYGB
TR:TREATMENT_SUMMARY             	microbiota transplantation once per week, but were otherwise handled like DIO
TR:TREATMENT_SUMMARY             	littermates. For fecal transplantation experiments, 100 mg of fresh stool from
TR:TREATMENT_SUMMARY             	body weight-stabilized RYGB donors was re-suspended in 1 ml of PBS, homogenized
TR:TREATMENT_SUMMARY             	carefully and administered via oral gavage with 200 μl of the suspension.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Solvents: Acetonitril (Merck KGaA, Darmstadt, Germany hypergrade for LC-MS)
SP:SAMPLEPREP_SUMMARY            	Water MiliQ, Extracting agent - ACN / H2O (1:1) Equipment 4 steel balls size M
SP:SAMPLEPREP_SUMMARY            	Eppendorf Tubes 2mL Tissue slicer (Rettberg, Germany) Centrifuge (Sigma) Work
SP:SAMPLEPREP_SUMMARY            	steps To approximately 100 mg of sample four steel balls of size M rae added in
SP:SAMPLEPREP_SUMMARY            	Eppendorf tubes. Then add for each mg of sample 5 µL of extracting agent. Shake
SP:SAMPLEPREP_SUMMARY            	the samples for 10 minutes at 30 Hz in the tissue slicer and centrifuge for 2
SP:SAMPLEPREP_SUMMARY            	minutes at 14000 rpm. The supernatant is used for targeted analytics as
SP:SAMPLEPREP_SUMMARY            	described in the kit manufactures manual. Kit measurement The analysis was
SP:SAMPLEPREP_SUMMARY            	performed using the validated Bile Acid Kit (Biocrates Life Sciences, Inssbruck,
SP:SAMPLEPREP_SUMMARY            	Austria) as described in Pham et al. 2017 [1]. Data processing is carried out
SP:SAMPLEPREP_SUMMARY            	with the provided quantitation method Kit (Biocrates Life Sciences AG,
SP:SAMPLEPREP_SUMMARY            	Innsbruck, Austria). 1 Pham HT, Arnhard K, Asad YJ, Deng L, Felder TK, St
SP:SAMPLEPREP_SUMMARY            	John-Williams L, et al. Inter-Laboratory Robustness of Next-Generation Bile Acid
SP:SAMPLEPREP_SUMMARY            	Study in Mice and Humans: International Ring Trial Involving 12 Laboratories.
SP:SAMPLEPREP_SUMMARY            	The Journal of Applied Laboratory Medicine 2019;1:129-42.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	UPLC (Waters Acquity, Waters Corporation, Milford, USA)
CH:COLUMN_NAME                   	none
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	ABI Sciex 5500 QTrap
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	Analyst version 1.6 Software from SCIEX. For Validation METIDQ Software version
MS:MS_COMMENTS                   	Boron from Biocrates
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	µM
MS_METABOLITE_DATA_START
Samples	1024038410	1024032390	1024035219	1024035295	1024035378	1024040970	1024041053	1024038481	1024038564	1024038424	1024032487	1024032401	1024035306	1024035223	1024040984	1024035382	1024038496	1024038579	1024038439	1024032491	1024032415	1024035311	1024035238	1024041072	1024038507	1024035397	1024040999	1024038443	1024038583	1024035242	1024035325	1024032420	1024032502	1024041087	1024038511	1024040927	1024041001	1024038458	1024038375	1024035330	1024035257	1024032517	1024032434	1024038526	1024041091	1024041015	1024040931	1024032366	1024038380	1024038609	1024040946	1024041020	1024041102	1024038531	1024032449	1024032521	1024035261	1024032453	1024032536	1024035276	1024035359	1024038613	1024038394	1024032371	1024038628	1024032385	1024038405	1024041034	1024040951	1024038545	1024038462	1024041049	1024040965	1024038550	1024038477	1024032541	1024032468	1024035363	1024035281	1024038632	1024039147	1024033282	1024033161	1024039185	1024033230	1024035931	1024033157	1024035896	1024041499	1024041533	1024041654	1024035979	1024041571	1024039050	1024039098	1024041529	1024039132	1024033195	1024033142	1024035964	1024041567	1024039128	1024033225	1024041640	1024033308	1024035926	1024039083	1024039045	1024039166	1024039201	1024041635	1024033211	1024041514	1024035998	1024033259	1024041552	1024041673	1024041591	1024039190	1024039079	1024039113	1024039151	1024039031	1024039109	1024035983	1024033123	1024033244	1024041548	1024041669	1024041621	1024033206	1024041586	1024035945	1024035907
Factors	Tissue:Serum | Rat_number:5 | Rat_sample_name:DIO_FMT_RYGB_1 | Sample treatment:DIO_FMT_RYGB	Tissue:Serum | Rat_number:15a | Rat_sample_name:RYGB_AB_1 | Sample treatment:RYGB_AB	Tissue:Serum | Rat_number:39 | Rat_sample_name:RYGB_AB_4 | Sample treatment:RYGB_AB	Tissue:Serum | Rat_number:54 | Rat_sample_name:Lean_5 | Sample treatment:Lean	Tissue:Serum | Rat_number:70 | Rat_sample_name:DIO_12 | Sample treatment:DIO	Tissue:Caecum | Rat_number:5 | Rat_sample_name:DIO_FMT_RYGB_1 | Sample treatment:DIO_FMT_RYGB	Tissue:Caecum | Rat_number:13 | Rat_sample_name:RYGB_AB_2 | Sample treatment:RYGB_AB	Tissue:Caecum | Rat_number:29 | Rat_sample_name:Lean_1 | Sample treatment:Lean	Tissue:Caecum | Rat_number:37 | Rat_sample_name:RYGB_Donor_5 | Sample treatment:RYGB_Donor	Tissue:Serum | Rat_number:6 | Rat_sample_name:DIO_AB_4 | Sample treatment:DIO_AB	Tissue:Serum | Rat_number:31 | Rat_sample_name:Lean_AB_3 | Sample treatment:Lean_AB	Tissue:Serum | Rat_number:17 | Rat_sample_name:RYGB_Donor_2 | Sample treatment:RYGB_Donor	Tissue:Serum | Rat_number:55 | Rat_sample_name:Lean_6 | Sample treatment:Lean	Tissue:Serum | Rat_number:40 | Rat_sample_name:RYGB_AB_5 | Sample treatment:RYGB_AB	Tissue:Caecum | Rat_number:6 | Rat_sample_name:DIO_AB_4 | Sample treatment:DIO_AB	Tissue:Serum | Rat_number:72 | Rat_sample_name:DIO_FMT_RYGB_7 | Sample treatment:DIO_FMT_RYGB	Tissue:Caecum | Rat_number:30 | Rat_sample_name:Lean_2 | Sample treatment:Lean	Tissue:Caecum | Rat_number:39 | Rat_sample_name:RYGB_AB_4 | Sample treatment:RYGB_AB	Tissue:Serum | Rat_number:8 | Rat_sample_name:DIO_FMT_RYGB_2 | Sample treatment:DIO_FMT_RYGB	Tissue:Serum | Rat_number:32 | Rat_sample_name:Lean_AB_4 | Sample treatment:Lean_AB	Tissue:Serum | Rat_number:18b | Rat_sample_name:RYGB_Donor_1 | Sample treatment:RYGB_Donor	Tissue:Serum | Rat_number:57 | Rat_sample_name:DIO_FMT_RYGB_8 | Sample treatment:DIO_FMT_RYGB	Tissue:Serum | Rat_number:41 | Rat_sample_name:RYGB_AB_6 | Sample treatment:RYGB_AB	Tissue:Caecum | Rat_number:15a | Rat_sample_name:RYGB_AB_1 | Sample treatment:RYGB_AB	Tissue:Caecum | Rat_number:31 | Rat_sample_name:Lean_AB_3 | Sample treatment:Lean_AB	Tissue:Serum | Rat_number:73 | Rat_sample_name:FMT_lean_5 | Sample treatment:FMT_lean	Tissue:Caecum | Rat_number:7 | Rat_sample_name:DIO_2 | Sample treatment:DIO	Tissue:Serum | Rat_number:9 | Rat_sample_name:DIO_3 | Sample treatment:DIO	Tissue:Caecum | Rat_number:40 | Rat_sample_name:RYGB_AB_5 | Sample treatment:RYGB_AB	Tissue:Serum | Rat_number:42 | Rat_sample_name:Lean_AB_5 | Sample treatment:Lean_AB	Tissue:Serum | Rat_number:58 | Rat_sample_name:FMT_lean_2 | Sample treatment:FMT_lean	Tissue:Serum | Rat_number:20 | Rat_sample_name:RYGB_Donor_3 | Sample treatment:RYGB_Donor	Tissue:Serum | Rat_number:33 | Rat_sample_name:Lean_3 | Sample treatment:Lean	Tissue:Caecum | Rat_number:17 | Rat_sample_name:RYGB_Donor_2 | Sample treatment:RYGB_Donor	Tissue:Caecum | Rat_number:32 | Rat_sample_name:Lean_AB_4 | Sample treatment:Lean_AB	Tissue:Serum | Rat_number:74 | Rat_sample_name:FMT_lean_6 | Sample treatment:FMT_lean	Tissue:Caecum | Rat_number:8 | Rat_sample_name:DIO_FMT_RYGB_2 | Sample treatment:DIO_FMT_RYGB	Tissue:Serum | Rat_number:10 | Rat_sample_name:DIO_4 | Sample treatment:DIO	Tissue:Serum | Rat_number:1 | Rat_sample_name:DIO_1 | Sample treatment:DIO	Tissue:Serum | Rat_number:63 | Rat_sample_name:FMT_lean_3 | Sample treatment:FMT_lean	Tissue:Serum | Rat_number:43 | Rat_sample_name:Lean_AB_6 | Sample treatment:Lean_AB	Tissue:Serum | Rat_number:34 | Rat_sample_name:Lean_4 | Sample treatment:Lean	Tissue:Serum | Rat_number:23 | Rat_sample_name:RYGB_AB_3 | Sample treatment:RYGB_AB	Tissue:Caecum | Rat_number:33 | Rat_sample_name:Lean_3 | Sample treatment:Lean	Tissue:Caecum | Rat_number:20 | Rat_sample_name:RYGB_Donor_3 | Sample treatment:RYGB_Donor	Tissue:Caecum | Rat_number:9 | Rat_sample_name:DIO_3 | Sample treatment:DIO	Tissue:Caecum | Rat_number:1 | Rat_sample_name:DIO_1 | Sample treatment:DIO	Tissue:Serum | Rat_number:11 | Rat_sample_name:DIO_FMT_RYGB_3 | Sample treatment:DIO_FMT_RYGB	Tissue:Serum | Rat_number:2 | Rat_sample_name:DIO_AB_1 | Sample treatment:DIO_AB	Tissue:Caecum | Rat_number:41 | Rat_sample_name:RYGB_AB_6 | Sample treatment:RYGB_AB	Tissue:Caecum | Rat_number:2 | Rat_sample_name:DIO_AB_1 | Sample treatment:DIO_AB	Tissue:Caecum | Rat_number:10 | Rat_sample_name:DIO_4 | Sample treatment:DIO	Tissue:Caecum | Rat_number:23 | Rat_sample_name:RYGB_AB_3 | Sample treatment:RYGB_AB	Tissue:Caecum | Rat_number:34 | Rat_sample_name:Lean_4 | Sample treatment:Lean	Tissue:Serum | Rat_number:28 | Rat_sample_name:Lean_AB_2 | Sample treatment:Lean_AB	Tissue:Serum | Rat_number:35 | Rat_sample_name:RYGB_Donor_4 | Sample treatment:RYGB_Donor	Tissue:Serum | Rat_number:47 | Rat_sample_name:DIO_AB_7 | Sample treatment:DIO_AB	Tissue:Serum | Rat_number:29 | Rat_sample_name:Lean_1 | Sample treatment:Lean	Tissue:Serum | Rat_number:36 | Rat_sample_name:RYGB_Donor_6 | Sample treatment:RYGB_Donor	Tissue:Serum | Rat_number:48 | Rat_sample_name:FMT_lean_1 | Sample treatment:FMT_lean	Tissue:Serum | Rat_number:64 | Rat_sample_name:FMT_lean_4 | Sample treatment:FMT_lean	Tissue:Caecum | Rat_number:42 | Rat_sample_name:Lean_AB_5 | Sample treatment:Lean_AB	Tissue:Serum | Rat_number:3 | Rat_sample_name:DIO_AB_2 | Sample treatment:DIO_AB	Tissue:Serum | Rat_number:12 | Rat_sample_name:DIO_FMT_RYGB_4 | Sample treatment:DIO_FMT_RYGB	Tissue:Caecum | Rat_number:43 | Rat_sample_name:Lean_AB_6 | Sample treatment:Lean_AB	Tissue:Serum | Rat_number:13 | Rat_sample_name:RYGB_AB_2 | Sample treatment:RYGB_AB	Tissue:Serum | Rat_number:4 | Rat_sample_name:DIO_AB_3 | Sample treatment:DIO_AB	Tissue:Caecum | Rat_number:11 | Rat_sample_name:DIO_FMT_RYGB_3 | Sample treatment:DIO_FMT_RYGB	Tissue:Caecum | Rat_number:3 | Rat_sample_name:DIO_AB_2 | Sample treatment:DIO_AB	Tissue:Caecum | Rat_number:35 | Rat_sample_name:RYGB_Donor_4 | Sample treatment:RYGB_Donor	Tissue:Caecum | Rat_number:27 | Rat_sample_name:Lean_AB_1 | Sample treatment:Lean_AB	Tissue:Caecum | Rat_number:12 | Rat_sample_name:DIO_FMT_RYGB_4 | Sample treatment:DIO_FMT_RYGB	Tissue:Caecum | Rat_number:4 | Rat_sample_name:DIO_AB_3 | Sample treatment:DIO_AB	Tissue:Caecum | Rat_number:36 | Rat_sample_name:RYGB_Donor_6 | Sample treatment:RYGB_Donor	Tissue:Caecum | Rat_number:28 | Rat_sample_name:Lean_AB_2 | Sample treatment:Lean_AB	Tissue:Serum | Rat_number:37 | Rat_sample_name:RYGB_Donor_5 | Sample treatment:RYGB_Donor	Tissue:Serum | Rat_number:30 | Rat_sample_name:Lean_2 | Sample treatment:Lean	Tissue:Serum | Rat_number:67 | Rat_sample_name:DIO_11 | Sample treatment:DIO	Tissue:Serum | Rat_number:49 | Rat_sample_name:DIO_AB_5 | Sample treatment:DIO_AB	Tissue:Caecum | Rat_number:47 | Rat_sample_name:DIO_AB_7 | Sample treatment:DIO_AB	Tissue:Caecum | Rat_number:138 | Rat_sample_name:Lean_9 | Sample treatment:Lean	Tissue:Serum | Rat_number:134 | Rat_sample_name:DIO_PF_FMT_2 | Sample treatment:DIO_PF_FMT	Tissue:Serum | Rat_number:123 | Rat_sample_name:DIO_FMT_RYGB_9 | Sample treatment:DIO_FMT_RYGB	Tissue:Serum | Rat_number:107 | Rat_sample_name:RYGB_Donor_7 | Sample treatment:RYGB_Donor	Tissue:Serum | Rat_number:124 | Rat_sample_name:DIO_PF_FMT_5 | Sample treatment:DIO_PF_FMT	Tissue:Caecum | Rat_number:57 | Rat_sample_name:DIO_FMT_RYGB_8 | Sample treatment:DIO_FMT_RYGB	Tissue:Serum | Rat_number:122 | Rat_sample_name:DIO_FMT_RYGB_10 | Sample treatment:DIO_FMT_RYGB	Tissue:Caecum | Rat_number:48 | Rat_sample_name:FMT_lean_1 | Sample treatment:FMT_lean	Tissue:Caecum | Rat_number:67 | Rat_sample_name:DIO_11 | Sample treatment:DIO	Tissue:Caecum | Rat_number:74 | Rat_sample_name:FMT_lean_6 | Sample treatment:FMT_lean	Tissue:Caecum | Rat_number:130 | Rat_sample_name:DIO_FMT_RYGB_11 | Sample treatment:DIO_FMT_RYGB	Tissue:Serum | Rat_number:100 | Rat_sample_name:Lean_8 | Sample treatment:Lean	Tissue:Caecum | Rat_number:114 | Rat_sample_name:RYGB_Donor_10 | Sample treatment:RYGB_Donor	Tissue:Caecum | Rat_number:129 | Rat_sample_name:DIO_10 | Sample treatment:DIO	Tissue:Caecum | Rat_number:133 | Rat_sample_name:DIO_PF_FMT_4 | Sample treatment:DIO_PF_FMT	Tissue:Caecum | Rat_number:73 | Rat_sample_name:FMT_lean_5 | Sample treatment:FMT_lean	Tissue:Caecum | Rat_number:100 | Rat_sample_name:Lean_8 | Sample treatment:Lean	Tissue:Serum | Rat_number:126 | Rat_sample_name:DIO_8 | Sample treatment:DIO	Tissue:Serum | Rat_number:119 | Rat_sample_name:RYGB_AB_8 | Sample treatment:RYGB_AB	Tissue:Caecum | Rat_number:64 | Rat_sample_name:FMT_lean_4 | Sample treatment:FMT_lean	Tissue:Caecum | Rat_number:109 | Rat_sample_name:RYGB_Donor_8 | Sample treatment:RYGB_Donor	Tissue:Caecum | Rat_number:99 | Rat_sample_name:Lean_7 | Sample treatment:Lean	Tissue:Serum | Rat_number:129 | Rat_sample_name:DIO_10 | Sample treatment:DIO	Tissue:Caecum | Rat_number:123 | Rat_sample_name:DIO_FMT_RYGB_9 | Sample treatment:DIO_FMT_RYGB	Tissue:Serum | Rat_number:99 | Rat_sample_name:Lean_7 | Sample treatment:Lean	Tissue:Caecum | Rat_number:55 | Rat_sample_name:Lean_6 | Sample treatment:Lean	Tissue:Caecum | Rat_number:132 | Rat_sample_name:DIO_PF_FMT_1 | Sample treatment:DIO_PF_FMT	Tissue:Caecum | Rat_number:128 | Rat_sample_name:DIO_7 | Sample treatment:DIO	Tissue:Serum | Rat_number:106 | Rat_sample_name:RYGB_Donor_9 | Sample treatment:RYGB_Donor	Tissue:Serum | Rat_number:114 | Rat_sample_name:RYGB_Donor_10 | Sample treatment:RYGB_Donor	Tissue:Caecum | Rat_number:122 | Rat_sample_name:DIO_FMT_RYGB_10 | Sample treatment:DIO_FMT_RYGB	Tissue:Serum | Rat_number:128 | Rat_sample_name:DIO_7 | Sample treatment:DIO	Tissue:Caecum | Rat_number:72 | Rat_sample_name:DIO_FMT_RYGB_7 | Sample treatment:DIO_FMT_RYGB	Tissue:Serum | Rat_number:139 | Rat_sample_name:Lean_10 | Sample treatment:Lean	Tissue:Serum | Rat_number:132 | Rat_sample_name:DIO_PF_FMT_1 | Sample treatment:DIO_PF_FMT	Tissue:Caecum | Rat_number:107 | Rat_sample_name:RYGB_Donor_7 | Sample treatment:RYGB_Donor	Tissue:Caecum | Rat_number:126 | Rat_sample_name:DIO_8 | Sample treatment:DIO	Tissue:Caecum | Rat_number:117 | Rat_sample_name:RYGB_AB_7 | Sample treatment:RYGB_AB	Tissue:Serum | Rat_number:109 | Rat_sample_name:RYGB_Donor_8 | Sample treatment:RYGB_Donor	Tissue:Caecum | Rat_number:125 | Rat_sample_name:DIO_PF_FMT_6 | Sample treatment:DIO_PF_FMT	Tissue:Caecum | Rat_number:135 | Rat_sample_name:DIO_PF_FMT_3 | Sample treatment:DIO_PF_FMT	Tissue:Caecum | Rat_number:139 | Rat_sample_name:Lean_10 | Sample treatment:Lean	Tissue:Caecum | Rat_number:127 | Rat_sample_name:DIO_9 | Sample treatment:DIO	Tissue:Caecum | Rat_number:134 | Rat_sample_name:DIO_PF_FMT_2 | Sample treatment:DIO_PF_FMT	Tissue:Serum | Rat_number:138 | Rat_sample_name:Lean_9 | Sample treatment:Lean	Tissue:Serum | Rat_number:117 | Rat_sample_name:RYGB_AB_7 | Sample treatment:RYGB_AB	Tissue:Serum | Rat_number:125 | Rat_sample_name:DIO_PF_FMT_6 | Sample treatment:DIO_PF_FMT	Tissue:Caecum | Rat_number:106 | Rat_sample_name:RYGB_Donor_9 | Sample treatment:RYGB_Donor	Tissue:Caecum | Rat_number:131 | Rat_sample_name:DIO_FMT_RYGB_12 | Sample treatment:DIO_FMT_RYGB	Tissue:Caecum | Rat_number:119 | Rat_sample_name:RYGB_AB_8 | Sample treatment:RYGB_AB	Tissue:Serum | Rat_number:127 | Rat_sample_name:DIO_9 | Sample treatment:DIO	Tissue:Caecum | Rat_number:111 | Rat_sample_name:RYGB_AB_9 | Sample treatment:RYGB_AB	Tissue:Caecum | Rat_number:58 | Rat_sample_name:FMT_lean_2 | Sample treatment:FMT_lean	Tissue:Caecum | Rat_number:49 | Rat_sample_name:DIO_AB_5 | Sample treatment:DIO_AB
Cholic Acid	0	0.126	0.755	4.44	0.801	1.08	20.6	1.77	0.303	0.028	0.015	3.48	4.39	0.026	17.4	0.243	2.97	42.9	0.335	0	0.185	0.214	0.472	10.8	0.704	0.735	0.197	0.513	2.42	0.302	0.513	7.09	1.84	0.115	1.12	2.69	1.76	0.021	1.26	0.196	0.005	0.591	0.096	0.209	0.463	0.816	2.01	0.187	0.093	10.9	12.2	3.06	8.19	0.208	0.006	10.6	0	2.01	3.85	0.108	2.35	21.3	0.004	2.38	10.6	4.04	0.115	7.3	20.2	12.1	0.334	8.33	17.9	4.75	0.492	4.54	1.87	1.39	0.46	7.11	0.249	3.2	6.27	20	1.8	5.01	0.356	2.93	16.1	29.9	3.76	2.88	0.438	5.69	7.48	2.2	0.293	5.28	0.077	1.17	0.56	0.328	0.25	2.52	0.183	0.823	16.2	4.12	0.093	1.11	9.27	1.6	9.4	4.76	0.072	0.399	1.31	25.4	3.42	1.56	13.1	1.1	1.56	1.7	1.48	0.083	1.47	NA	28.9	12.9	9.09	12.3	1.28	21.3
Chenodeoxycholic Acid	0.009	0.013	0.258	0.75	0.035	0	0.537	0	0	0.027	0.014	0.282	0.802	0.017	0.323	0.02	0	0	0.019	0.008	0.038	0.017	0.24	0.096	0	0.088	0	0.027	0	0.063	0.068	0.379	0.178	0	0	0.169	0	0.011	0.113	0.019	0.009	0.157	0.07	0.022	0	0	0	0.037	0.074	0.84	0.247	0	0.372	0	0.009	3.78	0.005	0.455	0.151	0.016	0.088	0.523	0.009	0.116	0.464	0.591	0.048	0	0.374	0.468	0	0.137	0.525	0	0	2.03	0.205	0.031	0.045	0.295	41.8	0.924	0.916	7.19	0.461	126	0.198	47.5	102	90.3	126	0.19	45.4	183	126	158	39.2	0.496	0	148	30.9	50.6	0.586	73.1	0.133	109	181	181	0	0.365	114	0.64	134	0.253	0.313	38.5	121	1.92	0.986	129	302	58.3	105	67.9	0.075	0	0.484	0	110	0	0.588	0	72.5	30.1
Deoxycholic Acid	0.061	0.007	0.638	0.113	0.08	NA	0.007	NA	1.13	0.029	0.058	0.136	0.231	0.013	NA	0.108	NA	NA	0.064	0.06	0.007	0.069	0.01	0.007	3.23	0.125	NA	0.105	NA	0.01	0.032	0.097	0.025	0.353	2.59	0.065	NA	0.08	0.119	0.096	0.021	0.185	0.007	0.134	2.43	NA	NA	0.169	0.112	0.018	NA	NA	0.007	NA	0.053	0.113	0.184	0.263	0.11	0.07	0.172	0.668	0.009	0.147	7.69	0.094	0.007	NA	1.6	NA	NA	NA	0.008	NA	3.53	0.257	0.265	0.157	0.055	NA	11.5	0.343	0	2.09	0.171	27	0.09	13.1	24.3	21.4	28.9	0.098	12.3	37.4	28.5	30.7	11.3	0.176	0	31.7	8.81	14.3	0.235	30.1	0.067	25.1	37.2	37	0	0.16	25.2	0.234	30	0.111	0.135	11.1	25.5	0.685	0.36	29.3	51.2	15.7	24.7	31.3	0.048	0	0.189	0.327	41.8	0	0.229	0.058	18.4	9.03
Glycocholic Acid	0.101	0.288	2.21	0.225	0.176	0	1.42	0	0.002	0.068	0.015	8.29	0.073	0.449	0.082	1.2	0	0.084	0.247	0.014	1.37	0.102	4.31	0.502	0.002	1.19	0	0.183	0.012	0.101	0.371	3.59	0.337	0.023	0	1.11	0	0.075	0.114	0.135	0.013	0.117	0.249	0	0.005	0.013	0.002	0.235	0	0.094	0.197	0	0.029	0	0.029	8.23	0.284	0.047	0.909	0.196	0.283	0.064	0.014	0.634	0.051	4.77	0.054	0	0.081	0	0	0.061	0.255	0.173	0.001	2.91	0.161	0.233	0.146	0.532	0.025	1.26	1.76	4.79	0.677	0.022	0.324	0.001	0.043	0.08	0.054	1.28	0.04	0.046	0.017	0.08	0.054	0.567	0.119	0.022	0.043	0.025	0.473	0.061	1.13	0.035	0.098	0.042	0.215	1.15	0.07	0.319	0	0.146	0.11	0.106	0.119	0.025	0.398	0.177	0.06	0.019	0.089	0.188	0.201	0.039	0.612	0.367	0.136	0.486	3.41	0.063	0.106	0.021
Glycochenodeoxycholic Acid	0.009	0.047	0.085	0.023	0.006	0	0.031	0	0.001	0.012	0.008	0.456	0.012	0.025	0.002	0.032	0	0.006	0.015	0.007	0.109	0.008	0.926	0.055	0.001	0.058	0	0.012	0	0.015	0.016	0.132	0.013	0.001	0.001	0.046	0	0.006	0.011	0.017	0.009	0.015	0.038	0	0	0	0	0.014	0.009	0.027	0.014	0	0.005	0	0.007	2.5	0.023	0.008	0.025	0.011	0.008	0.006	0.004	0.017	0.004	0.23	0.008	0	0	0	0.001	0	0.008	0.011	0.001	0.389	0.014	0.007	0.012	0.02	0.008	0.035	0.156	0.484	0.075	0	0.014	0	0	0.008	0	0.054	0.011	0	0	0.016	0.008	0.053	0.015	0	0.012	0.008	0.018	0.007	0.041	0	0	0	0.019	0.109	0.007	0.015	0.006	0.037	0.008	0.021	0.016	0	0.077	0.021	0	0.011	0.007	0	0.023	0.008	0.042	0.007	0	0.01	0.162	0.006	0	0
Glycodeoxycholic Acid	0.006	0	0.053	0.009	0.005	0.004	0.008	0.003	0	0.004	0.003	0.38	0.014	0.004	0.007	0.019	0.002	0.022	0.009	0.002	0	0.007	0	0	0.002	0.051	0.005	0.012	0.006	0.001	0.003	0.073	0.002	0.001	0	0.014	0.009	0.009	0.015	0.006	0.002	0.01	0	0	0.003	0.014	0.004	0.009	0.005	0.003	0.013	0.004	0	0.002	0.003	0.154	0.011	0.008	0.042	0.004	0.026	0.002	0.002	0.014	0.003	0.075	0.001	0.013	0.004	0.002	0	0.011	0	0.015	0.002	0.271	0.028	0.005	0.004	0.028	0	0.074	0.114	1.44	0.052	0.005	0.005	0.006	0.007	0.007	0.021	0.006	0.006	0.026	0.017	0.027	0.001	0.047	0	0.018	0.011	0.001	0.055	0.01	0.005	0.011	0.032	0.019	0	0.127	0	0.01	0.01	0.003	0	0.016	0.029	0.006	0.145	0.028	0.035	0	0.008	0.017	0.003	0	0.043	0.007	0.044	0	0.082	0.003	0.002	0.004
Glycolithocholic Acid	0.002	0	0.001	0.002	0.001	0.002	0.001	0.002	0	0.001	0	0.008	0.001	0.001	0	0.001	0.003	0.002	0.003	0.001	0	0.001	0.001	0	0.001	0.004	0.003	0.001	0.001	0.002	0.002	0.002	0.001	0	0	0.002	0.004	0.001	0.003	0.002	0.002	0	0	0.001	0.001	0.001	0.005	0.001	0.002	0.004	0.003	0.001	0	0.004	0.001	0.006	0.003	0	0.001	0	0.002	0	0.003	0.001	0.001	0.001	0.003	0.004	0	0.002	0	0.004	0	0	0.001	0.006	0	0.001	0.003	0.001	0	0.003	0.002	0.018	0.002	0.002	0.001	0	0.01	0.001	0.003	0	0.001	0.003	0.003	0.003	0.001	0.001	0	0.004	0	0.003	0	0.002	0	0.004	0.005	0.003	0	0.002	0.002	0	0.012	0	0	0.002	0.001	0.002	0.006	0.005	0.004	0	0	0.003	0	0	0.002	0.007	0.004	0.001	0.003	0	0	0
Glycoursodeoxycholic Acid	0.002	0.005	0.02	0.007	0.011	0.005	0.015	0.001	0.003	0.002	0.002	3.62	0.035	0.004	0.002	0.092	0.001	0.008	0.002	0.002	0.024	0.012	0.052	0.022	0.001	0.095	0.004	0.037	0.009	0.002	0.03	0.718	0.003	0.008	0.002	0.056	0.002	0.01	0.026	0.018	0.002	0.003	0.006	0.002	0.003	0.009	0.005	0.022	0.002	0.008	0.004	0.003	0.011	0	0.002	0.391	0.003	0.013	0.004	0.017	0.034	0.002	0.002	0.052	0.004	2.11	0.001	0.008	0.001	0.002	0.003	0.01	0.004	0.008	0.001	0.409	0.029	0.017	0.002	0.016	0.013	0.007	0.272	0.718	0.125	0.006	0.046	0.005	0.018	0.019	0.025	0.19	0.021	0.019	0.014	0.024	0.019	0.109	0.007	0.013	0.036	0.011	0.03	0.021	0.159	0.022	0.015	0.02	0.01	0.188	0.011	0.023	0.025	0.03	0.017	0.024	0.034	0.011	0.09	0.044	0.01	0.014	0.035	0.009	0.029	0.006	0.085	0.01	0.04	0.007	0.382	0.007	0.026	0.007
Hyodeoxycholic Acid	0.013	0.013	0.024	0.028	0.02	0.102	0.148	0.299	0.067	0.016	0.013	0.056	0.051	0.014	0.15	0.022	0.609	0.37	0.013	0.013	0.016	0.016	0.022	0.017	0.036	0.023	0.399	0.027	0.086	0.015	0.016	0.067	0.015	0.059	0.027	0.022	0.087	0.017	0.02	0.018	0.013	0.025	0.015	0.017	0.202	1.4	0.567	0.021	0.017	0.095	0.095	0.27	0.024	0.265	0.013	0.164	0.013	0.041	0.02	0.017	0.026	0.101	0.013	0.024	0.076	0.039	0.016	0.484	0.114	0.633	0.06	0.707	0.134	0.113	0.028	0.074	0.034	0.028	0.015	0.07	4.25	0.018	0.123	1.48	0.079	4.24	0.056	1.95	7.32	4.72	22.9	0.093	3.69	9.97	15.5	14.3	4.52	0.061	0.007	13.4	1.56	5.69	0.018	17.6	0.07	8.85	20.5	13.2	0.005	0.129	8.79	0.055	14.3	0.124	0.053	1.83	11	0.266	0.151	15.7	4.35	8.7	18.1	2.89	0.093	0.004	0.094	0.583	21.8	0.325	0.114	0.05	6.73	0.461
Lithocholic Acid	0.003	0	0	0.009	0.005	2.86	0	1.77	0.674	0	0	0	0.019	0	0.337	0	2.35	5.36	0	0	0	0	0	0	0.186	0.007	2.15	0	0.21	0	0	0	0.005	0.04	0.115	0	1.37	0	0	0	0	0	0	0.069	0.439	3.83	2.83	0	0.011	0	0.513	0.853	0.92	5.74	0	0	0.008	0.01	0	0	0	0	0	0	0.307	0	0	2.66	0.052	0.771	0.323	5.3	0	1.12	0.092	0	0.049	0	0.024	0.847	4.2	0	0	0.046	0	3.54	0	1.41	2.58	3.05	7.4	0.097	3.03	5.24	4.61	7.89	3.02	0	0	6.07	2.78	3.01	0	7.1	0	6.79	6.52	6.48	0	0.001	4.12	0	4.66	0	0.117	3.52	5.15	0.052	0.211	5.56	3.43	5	3.86	4.09	0.024	0	0	0.135	7.97	0	0	0	4.57	0.355
α-Muricholic Acid	0.073	0.027	0.233	0.614	0.046	2.03	8.05	16.2	2.94	0.302	0.014	0.25	0.672	0.018	11.8	0.008	28.1	82.8	0.17	0.007	0.029	0.006	0.154	2.37	11.1	0.042	0	0.238	14.5	0.045	0.038	0.731	0.152	0	11.4	0.488	2	0.041	0.06	0.008	0.007	0.056	0.029	1.61	0	0.286	0.812	0.008	0.028	6.05	6.77	0.215	2.86	10	0.009	6.81	0.024	0.26	0.116	0.008	0.049	34.1	0.009	0.103	16.4	0.41	0.502	0.499	19.1	41.1	21.2	1.53	9.71	43.8	7.19	0.087	0.151	0.027	0.018	21.3	0.96	0.049	0.423	0.904	0.113	0.505	0.017	0.707	1.74	NA	6.43	0.299	0.153	5.55	4.99	3.65	0.173	0.337	0.011	1.58	0.331	0.146	0.011	1.8	0.015	0.264	5.36	5.56	0.022	0.061	0.895	0.053	0.6	0.892	0.005	1.94	0.809	4.79	0.173	0.98	NA	0.438	0.018	2.1	0.25	0.011	0.038	NA	NA	3.03	0.356	3.68	0.593	3.23
β-Muricholic Acid	0.042	0.105	1.47	0.694	0.229	25.8	31.4	10.8	1.75	0.223	0.122	1.12	1.39	0.137	35	0.093	18.2	55.8	0.11	0.082	0.776	0.068	0.136	2.36	7.94	0.084	8.04	0.154	10.1	0.294	0.058	1.82	0	0.743	7.72	0.344	27	0.02	0.163	0.049	0.03	0.305	0.079	1.11	2.41	22.8	17.5	0.133	0.625	4.49	22.8	6.82	1.72	6.49	0.065	1.62	0.265	0.811	0.464	0.049	0.092	23.2	0.056	0.332	12.2	1.57	0.311	14	29.6	28.8	13.1	16	30	29.6	4.96	0.487	0.282	0.217	0.223	15.9	11.6	0.33	0.743	2.51	0.12	11.8	0.18	7.45	13.9	36.7	34.8	0.639	4.33	47.1	33.8	30.8	10.8	0.016	0.391	15.1	2.53	10.2	0.095	21.8	0.059	11.7	49.5	37.3	0.249	0.264	32.4	0.223	24.2	0.548	0.031	1.88	11.9	18.6	0.289	21.1	52.4	13.6	8.98	21.2	0.283	0.123	0.126	41	60.7	18.4	0.273	6.09	19.4	19.5
ω-Muricholic Acid	0.019	0.025	0.063	0.157	0.044	16.1	11.4	10.9	2.91	0.017	0.006	0.722	0.332	0.005	10.3	0.025	15.8	4.47	0.065	0.005	0.073	0.011	0.012	1.8	1.11	0.015	17.3	0.018	3.12	0.009	0.017	0.436	0.034	2.21	0.826	0.079	28.2	0.015	0.049	0.032	0.002	0.257	0.002	2.73	9.02	9.92	15.4	0.082	0.028	3.43	1.63	4.06	2.27	35.8	0.002	0.631	0.017	0.074	0.072	0.019	0.04	4.01	0.008	0.048	0.864	0.62	0.019	14.1	10.6	9.81	1.09	13	2.92	17.8	0.831	0.162	0.091	0.068	0.005	1.94	16.6	0.461	0.106	1.5	0.042	7.85	0.048	5.17	5.34	18.6	20	0.097	15.4	44.8	38.2	19.1	8.36	0.085	0.011	19.4	10.1	7.52	0.063	27.2	0.022	24.3	35.8	29.6	0.003	0.068	29.5	0.059	14.5	0.049	0.016	23	21.6	1.38	0.208	31.9	73.2	10.1	12.6	55.6	0.029	0.001	0.11	2.97	38.6	0.926	0.051	0.497	14.8	0.748
Taurocholic Acid	4.33	7.2	4.7	2.32	0	0.273	14.8	0.05	0.009	2.4	2.86	2.57	0.5	4.16	9.75	6.89	0.043	6.84	5.71	2.48	16.1	3.56	2.46	16.6	2.87	5.78	0.088	1.71	0.387	3.64	0.763	1.34	2.58	0	0.314	8.08	0.28	2.74	1.29	7.34	1.64	2.11	6.43	0.057	0.013	0.422	0.075	7.86	5.3	0.185	14	0.108	8.4	0.028	2.16	2.93	11.6	1.17	1.86	5.38	2.28	0.353	0.666	4.61	7.7	3.6	3.23	0.156	4.99	0.1	0.102	0.648	10.8	0.839	0.091	0.834	0.46	5.94	5.31	13	0.159	3.14	2.91	0.77	0.302	0.722	1.91	0.058	1.3	0.853	1.13	6.99	0.209	0.368	0.352	1.03	0.224	0.36	2.06	0.249	0.078	0.077	1.66	5.98	2.75	0.268	2.36	0.215	4.96	0.848	0.552	0.63	0.727	0.739	0.765	0.058	0.059	1.27	0.162	0.209	0.111	0.14	0.213	0.876	0.419	0.43	1.05	27	1.31	13.8	4.36	7.03	0.404	1.77
Taurochenodeoxycholic Acid	0.243	1.45	0.511	0.37	0.161	0.007	0.386	0.006	0.001	0.444	0.333	0.373	0.11	0.434	0.062	0.468	0.005	0.006	0.525	0.294	2.45	0.232	0.409	3.16	0.025	0.597	0.001	0.199	0.012	0.562	0.173	0.19	0.417	0	0.012	0.643	0.01	0.149	0.167	0.737	0.289	0.528	1.1	0.006	0.002	0.018	0.002	0.644	0.279	0.008	0.128	0.006	0.101	0.007	0.202	1.34	1.37	0.268	0.128	0.39	0.144	0.069	0.088	0.343	0.182	0.758	0.195	0.006	0.018	0.006	0.015	0.033	0.098	0.021	0.009	0.244	0.074	0.308	0.459	1.3	0.039	0.165	0.323	0.244	0.079	0.036	0.17	0.013	0.043	0.09	0.193	0.989	0.059	0.016	0.053	0.126	0.047	0.049	0.211	0.025	0.023	0.026	0.125	0.068	0.505	0.068	0.231	0.031	0.431	0.188	0.034	0.085	0.063	0.216	0.113	0.027	0.013	0.065	0.051	0.026	0.024	0.044	0.024	0.051	0.128	0.045	0.113	0.142	0.113	0.042	0.205	0.143	0.081	0.029
Taurodeoxycholic Acid	0.162	1.27	0.344	0.068	0.072	0.011	0	0.007	0.002	0.041	0.075	0.111	0.044	0.058	0.059	0.303	0.006	0.074	0.224	0.069	0	0.176	0	0	0.037	0.297	0.009	0.127	0.005	0.007	0.021	0.042	0.038	0.001	0.003	0.112	0.014	0.286	0.112	0.159	0.009	0.252	0	0.002	0.002	0.022	0.007	0.379	0.106	0.002	0.174	0.008	0	0.003	0.072	0.029	0.513	0.149	0.126	0.159	0.194	0	0.005	0.211	0.017	0.15	0	0.008	0	0.007	0.005	0.031	0	0.038	0.004	0.074	0.069	0.242	0.064	1.05	0.01	0.278	0.116	0.144	0.032	0.053	0.082	0.008	0.056	0.059	0.19	0.172	0.039	0.404	0.049	0.089	0.015	0.025	0.003	0.033	0.032	0.008	0.289	0.034	0.096	0.028	1.06	0.144	0.443	0.088	0.14	0.099	0.065	0.035	0.11	0.007	0.006	0.03	0.051	0.035	0.209	0.016	0.004	0.124	0.034	0.003	0.099	0.004	0.38	0.003	0.092	0.003	0.022	0.029
Taurolithocholic Acid	0.001	0	0.005	0.003	0.002	0.002	0	0.001	0	0.001	0	0.009	0.002	0.001	0.001	0.002	0.002	0.008	0.001	0	0.001	0.002	0.002	0.003	0	0.004	0.001	0.003	0.001	0	0.002	0.004	0.001	0	0	0.003	0.001	0.002	0.002	0.002	0	0.005	0.001	0	0	0.002	0.001	0.002	0.002	0.004	0.002	0	0	0.001	0.001	0.003	0.009	0.002	0.002	0.003	0.004	0	0.001	0.002	0.001	0.01	0.001	0.002	0	0	0	0.003	0	0	0	0.006	0.002	0.002	0.002	0.023	0.003	0.003	0.004	0.011	0.003	0.004	0.002	0.003	0.008	0.007	0.015	0.005	0.004	0.015	0	0.01	0.003	0.003	0.002	0.006	0.004	0.004	0.002	0.006	0.004	0.007	0.025	0.01	0.003	0.003	0.007	0.002	0.009	0.005	0.003	0.003	0.006	0.003	0.005	0	0.011	0.004	0	0.01	0.005	0.002	0.004	0.005	0.015	0.003	0.006	0.021	0.005	0.002
α-β-Tauromuricholic Acid	1.54	NA	1.16	0.755	0.437	0.091	3.52	0.194	0.013	2.12	1.78	2.28	0.316	2.08	7.59	1.72	0.073	2.51	2.67	1.44	NA	0.78	0.631	4.69	0.439	1.17	0.043	0.574	0.164	2.06	0.283	0.969	0.886	0.033	0.591	2.02	0.119	0.544	0.35	2.6	0.632	1.03	3.33	0.109	0.021	0.313	0.029	2.84	1.76	0.035	NA	0.089	2.74	0.043	0.793	7.26	NA	0.641	0.318	1.26	0.315	0.219	0.333	0.949	2.46	3.03	1.35	0.057	5.24	1.42	0.115	0.258	NA	0.175	0.055	0.351	0.159	1.17	2.87	NA	0.182	0.812	1.48	1.2	0.16	0.532	0.947	0.038	0.774	1.37	1.76	4.73	0.274	3.74	0.471	0.525	0.271	0.138	1.05	0.151	0.098	0.112	0.604	0.423	2.39	0.498	2.94	0.275	3.92	0.424	1.31	0.295	0.452	0.676	0.326	0.115	0.042	1.41	0.131	0.129	0.263	0.212	0.151	0.708	0.471	0.194	0.302	5.55	1.66	1.69	0.879	4.25	0.216	NA
Tauroursodeoxycholic Acid	0.103	0.193	0.073	0.124	0.285	0.013	0.206	0.033	0.007	0.052	0.064	3.03	0.346	0.043	0.037	1.17	0.032	0.022	0.129	0.043	0.613	0.433	0.022	1.95	0.025	0.885	0.018	0.77	0.011	0.115	0.147	1.07	0.035	0.017	0.012	0.82	0.009	0.4	0.405	1.02	0.022	0.201	0.078	0.004	0.015	0.14	0.022	1.11	0.056	0.004	0.074	0.02	0.073	0.009	0.028	0.479	0.533	0.505	0.025	0.68	0.603	0.035	0.013	1.01	0.06	4.83	0.043	0.019	0.017	0.021	0.014	0.201	0.068	0.022	0.006	0.219	0.183	0.917	0.086	1.16	0.078	0.065	0.862	0.531	0.118	0.14	0.63	0.022	0.272	0.289	0.707	1.75	0.187	0.347	0.144	0.264	0.11	0.107	0.031	0.1	0.066	0.061	0.345	0.226	1.05	0.208	1.09	0.122	0.068	0.329	0.237	0.19	0.158	0.21	0.294	0.071	0.026	0.027	0.074	0.099	0.025	0.083	0.095	0	0.145	0.012	0.222	0.07	0.946	0.032	0.792	0.06	0.089	0.046
Ursodeoxycholic Acid	0	0	0.169	2.15	1.43	23.8	0.479	63.2	10.1	0	0	24.6	13.7	0	0.035	1.85	87	5.37	0	0	0.095	0.865	0	0	0	2.61	119	3.56	0	0	0.678	22	0.096	14.8	0	2.34	10.9	0.758	3.37	1.03	0	1.1	0	1.23	48.5	162	128	2.1	0	0	0.183	83.4	0	53.5	0	1.12	0.142	7.02	0.055	1.24	3.76	0	0	4.95	0	25.8	0	101	0.121	4.13	0	176	0	7.61	0	8.99	5.18	3.85	0	6.15	179	0.65	26.4	64.1	7.79	261	3.57	110	241	193	435	7.55	189	374	420	448	198	12	0	405	101	290	1.67	515	3.76	353	548	522	0	8.7	339	3.29	361	9.3	2.46	97.6	477	2.2	11.2	499	254	291	468	146	4.21	0	6.27	1.75	697	0.479	17.5	0.843	223	0.58
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	Name	KEGG
Cholic Acid	CA	C00695
Chenodeoxycholic Acid	CDCA	C02528
Deoxycholic Acid	DCA	C04483
Glycocholic Acid	GCA	C01921
Glycochenodeoxycholic Acid	GCDCA	C05466
Glycodeoxycholic Acid	GDCA	C05464
Glycolithocholic Acid	GLCA	C15557
Glycoursodeoxycholic Acid	GUDCA
Hyodeoxycholic Acid	HDCA	C15517
Lithocholic Acid	LCA	C03990
α-Muricholic Acid	MCA(a)	C17647
β-Muricholic Acid	MCA(b)	C17726
ω-Muricholic Acid	MCA(o)	C17727
Taurocholic Acid	TCA	C02258
Taurochenodeoxycholic Acid	TCDCA	C05465
Taurodeoxycholic Acid	TDCA	C05463
Taurolithocholic Acid	TLCA	C02592
α-β-Tauromuricholic Acid	TMCA(a+b)
Tauroursodeoxycholic Acid	TUDCA	C16868
Ursodeoxycholic Acid	UDCA	C07880
METABOLITES_END
#END