#METABOLOMICS WORKBENCH sczhang_20210701_135547_mwtab.txt DATATRACK_ID:2736 STUDY_ID:ST001868 ANALYSIS_ID:AN003030 PROJECT_ID:000000 VERSION 1 CREATED_ON July 2, 2021, 11:25 am #PROJECT PR:PROJECT_TITLE Sodium dichloroacetate stimulates cardiac mitochondrial metabolism and improves PR:PROJECT_TITLE cardiac conduction in the ovine fetus during labor PR:PROJECT_TYPE untargeted NMR analysis PR:PROJECT_SUMMARY Previous studies in our laboratory have suggested that the increase in PR:PROJECT_SUMMARY stillbirth in pregnancies complicated by chronic maternal stress or PR:PROJECT_SUMMARY hypercortisolemia is associated with cardiac dysfunction in late stages of labor PR:PROJECT_SUMMARY and delivery. Transcriptomics analysis of the overly represented differentially PR:PROJECT_SUMMARY expressed genes in the fetal heart of hypercortisolemic ewes indicated PR:PROJECT_SUMMARY involvement of mitochondrial function. Sodium dichloroacetate (DCA) has been PR:PROJECT_SUMMARY used to improve mitochondrial function in several disease states. We PR:PROJECT_SUMMARY hypothesized that administration of DCA to laboring ewes would improve both PR:PROJECT_SUMMARY cardiac mitochondrial activity and cardiac function in their fetuses. Four PR:PROJECT_SUMMARY groups of ewes and their fetuses were studied: control, cortisol-infused (1 PR:PROJECT_SUMMARY g/kg/d from 115 to term; CORT), DCA-treated (over 24h) or DCA+CORT-treated; PR:PROJECT_SUMMARY oxytocin was delivered starting 48h before the DCA treatment. DCA significantly PR:PROJECT_SUMMARY decreased cardiac lactate, alanine and glucose/glucose-6-phosphate and increased PR:PROJECT_SUMMARY acylcarnitine/isobutyryl-carnitine. DCA increased mitochondrial activity, PR:PROJECT_SUMMARY increasing oxidative phosphorylation (PCI, PCI+II)) per tissue weight or per PR:PROJECT_SUMMARY unit of citrate synthase. DCA also decreased the duration of the QRS, PR:PROJECT_SUMMARY attenuating the prolongation of the QRS observed in CORT fetuses. The effect to PR:PROJECT_SUMMARY reduce QRS duration with DCA treatment correlated with increased PR:PROJECT_SUMMARY glycerophosphocholine and serine and decreased phophocholine after DCA PR:PROJECT_SUMMARY treatment. There were negative correlations of PR:PROJECT_SUMMARY acylcarnitine/isobutyryl-carnitine to both HR and MAP. These results suggest PR:PROJECT_SUMMARY that improvements in mitochondrial respiration with DCA produced changes in the PR:PROJECT_SUMMARY cardiac lipid metabolism that favor improved conduction in the heart. DCA may PR:PROJECT_SUMMARY therefore be an effective treatment of fetal cardiac metabolic disturbances in PR:PROJECT_SUMMARY labor that can contribute to impairments of fetal cardiac conduction. PR:INSTITUTE University of Georgia PR:DEPARTMENT Biochemistry and Molecular Biology and Complex Carbohydrate Research Center, PR:DEPARTMENT Department of Pharmacodynamics (University of Florida), Department of Physiology PR:DEPARTMENT and Functional Genomics (University of Florida) PR:LABORATORY Edison Lab, Keller-Wood Lab, and Wood Lab PR:LAST_NAME Edison PR:FIRST_NAME Arthur PR:ADDRESS 315 Riverbend Road, Complex Carbohydrate Research Center, ATHENS, GA, 30605, USA PR:EMAIL aedison@uga.edu PR:PHONE NA PR:CONTRIBUTORS Serene Joseph, Mengchen Li, Sicong Zhang, Lloyd Horne, Peter. W. Stacpoole, PR:CONTRIBUTORS Stephanie E. Wohlgemuth, Arthur S. Edison, Charles Wood, Maureen Keller-Wood #STUDY ST:STUDY_TITLE Sodium dichloroacetate stimulates cardiac mitochondrial metabolism and improves ST:STUDY_TITLE cardiac conduction in the ovine fetus during labor (part II) ST:STUDY_TYPE untargeted NMR analysis-noesy ST:STUDY_SUMMARY Previous studies in our laboratory have suggested that the increase in ST:STUDY_SUMMARY stillbirth in pregnancies complicated by chronic maternal stress or ST:STUDY_SUMMARY hypercortisolemia is associated with cardiac dysfunction in late stages of labor ST:STUDY_SUMMARY and delivery. Transcriptomics analysis of the overly represented differentially ST:STUDY_SUMMARY expressed genes in the fetal heart of hypercortisolemic ewes indicated ST:STUDY_SUMMARY involvement of mitochondrial function. Sodium dichloroacetate (DCA) has been ST:STUDY_SUMMARY used to improve mitochondrial function in several disease states. We ST:STUDY_SUMMARY hypothesized that administration of DCA to laboring ewes would improve both ST:STUDY_SUMMARY cardiac mitochondrial activity and cardiac function in their fetuses. Four ST:STUDY_SUMMARY groups of ewes and their fetuses were studied: control, cortisol-infused (1 ST:STUDY_SUMMARY g/kg/d from 115 to term; CORT), DCA-treated (over 24h) or DCA+CORT-treated; ST:STUDY_SUMMARY oxytocin was delivered starting 48h before the DCA treatment. DCA significantly ST:STUDY_SUMMARY decreased cardiac lactate, alanine and glucose/glucose-6-phosphate and increased ST:STUDY_SUMMARY acylcarnitine/isobutyryl-carnitine. DCA increased mitochondrial activity, ST:STUDY_SUMMARY increasing oxidative phosphorylation (PCI, PCI+II)) per tissue weight or per ST:STUDY_SUMMARY unit of citrate synthase. DCA also decreased the duration of the QRS, ST:STUDY_SUMMARY attenuating the prolongation of the QRS observed in CORT fetuses. The effect to ST:STUDY_SUMMARY reduce QRS duration with DCA treatment correlated with increased ST:STUDY_SUMMARY glycerophosphocholine and serine and decreased phophocholine after DCA ST:STUDY_SUMMARY treatment. There were negative correlations of ST:STUDY_SUMMARY acylcarnitine/isobutyryl-carnitine to both HR and MAP. These results suggest ST:STUDY_SUMMARY that improvements in mitochondrial respiration with DCA produced changes in the ST:STUDY_SUMMARY cardiac lipid metabolism that favor improved conduction in the heart. DCA may ST:STUDY_SUMMARY therefore be an effective treatment of fetal cardiac metabolic disturbances in ST:STUDY_SUMMARY labor that can contribute to impairments of fetal cardiac conduction. ST:INSTITUTE University of Georgia ST:DEPARTMENT Biochemistry and Molecular Biology and Complex Carbohydrate Research Center, ST:DEPARTMENT Department of Pharmacodynamics (University of Florida), Department of Physiology ST:DEPARTMENT and Functional Genomics (University of Florida) ST:LABORATORY Edison Lab, Keller-Wood Lab, and Wood Lab ST:LAST_NAME Zhang ST:FIRST_NAME Sicong ST:ADDRESS 315 Riverbend Road, Complex Carbohydrate Research Center ST:EMAIL sz91614@uga.edu ST:PHONE 7067151662 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 29 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Ovis aries SU:TAXONOMY_ID 9940 SU:ANIMAL_INCLUSION_CRITERIA Pregnant #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS 1682 1682_L Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/39; Weight(mg)=30.01; MAPavglast3h=NaN; HRavglast3h=137; QRSAvg46=34; QRSavglast3=38; GA_surg=-43150; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=m; fetus=<undefined>; breed=19-Feb-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=09-Jul-2018 SUBJECT_SAMPLE_FACTORS 1428 1428_L Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/46; Weight(mg)=36.77; MAPavglast3h=49; HRavglast3h=150; QRSAvg46=41; QRSavglast3=48; GA_surg=128; GA_cort=116; GA_sac=143; cutofftime=26-Apr-2018 09:15:00; gender=m; fetus=s; breed=04-Dec-2017; cortdate=30-Mar-2018; surgery=11-Apr-2018; oxytocin=23-Apr-2018; oxytocintime=NaN; sac=26-Apr-2018 SUBJECT_SAMPLE_FACTORS 1775 1775_L Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/40; Weight(mg)=34.43; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=126; GA_cort=-; GA_sac=140; cutofftime=19-Dec-2017 08:09:00; gender=f; fetus=triplet; breed=01-Aug-2017; cortdate=no; surgery=05-Dec-2017; oxytocin=17-Dec-2018; oxytocintime=NaN; sac=19-Dec-2017 SUBJECT_SAMPLE_FACTORS 1427 1427_L Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/41; Weight(mg)=34.52; MAPavglast3h=52; HRavglast3h=129; QRSAvg46=41; QRSavglast3=45; GA_surg=123; GA_cort=-; GA_sac=142; cutofftime=NaT; gender=m; fetus=<undefined>; breed=03-Sep-2018; cortdate=no; surgery=04-Jan-2019; oxytocin=NaT; oxytocintime=NaN; sac=23-Jan-2019 SUBJECT_SAMPLE_FACTORS 1329 1329_L Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/5; Weight(mg)=29.91; MAPavglast3h=55; HRavglast3h=164; QRSAvg46=NaN; QRSavglast3=46; GA_surg=122; GA_cort=115; GA_sac=141; cutofftime=NaT; gender=m; fetus=<undefined>; breed=24-Sep-2018; cortdate=17-Jan-2019; surgery=24-Jan-2019; oxytocin=NaT; oxytocintime=NaN; sac=12-Feb-2019 SUBJECT_SAMPLE_FACTORS 1261 1261_L Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/42; Weight(mg)=34.58; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=116; GA_sac=142; cutofftime=NaT; gender=m; fetus=s; breed=25-Sep-2017; cortdate=19-Jan-2018; surgery=30-Jan-2018; oxytocin=11-Feb-2018; oxytocintime=NaN; sac=14-Feb-2018 SUBJECT_SAMPLE_FACTORS 1668 1668_L Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/7; Weight(mg)=22.7; MAPavglast3h=56; HRavglast3h=139; QRSAvg46=36; QRSavglast3=38; GA_surg=120; GA_cort=115; GA_sac=140; cutofftime=NaT; gender=f; fetus=<undefined>; breed=25-Jun-2018; cortdate=18-Oct-2018; surgery=23-Oct-2018; oxytocin=NaT; oxytocintime=NaN; sac=12-Nov-2018 SUBJECT_SAMPLE_FACTORS 1611 1611_L Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/44; Weight(mg)=38.19; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=130; GA_cort=-; GA_sac=144; cutofftime=NaT; gender=f; fetus=s; breed=15-Jan-2018; cortdate=no; surgery=25-May-2018; oxytocin=05-Jun-2018; oxytocintime=NaN; sac=08-Jun-2018 SUBJECT_SAMPLE_FACTORS 1254 1254_L Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/10; Weight(mg)=47.54; MAPavglast3h=56; HRavglast3h=139; QRSAvg46=37; QRSavglast3=37; GA_surg=123; GA_cort=-; GA_sac=142; cutofftime=01-Nov-2017 07:47:00; gender=m; fetus=s; breed=12-Jun-2017; cortdate=no; surgery=13-Oct-2017; oxytocin=29-Oct-2017; oxytocintime=NaN; sac=01-Nov-2017 SUBJECT_SAMPLE_FACTORS 1565 1565_L Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/11; Weight(mg)=28.57; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-43458; GA_cort=-43458; GA_sac=147; cutofftime=NaT; gender=m; fetus=<undefined>; breed=24-Dec-2018; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=20-May-2019 SUBJECT_SAMPLE_FACTORS 1719 1719_L Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/12; Weight(mg)=39.05; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-; GA_cort=-; GA_sac=NaN; cutofftime=NaT; gender=m; fetus=<undefined>; breed=NaT; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=0.3125; sac=NaT SUBJECT_SAMPLE_FACTORS 1212 1212_L Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/13; Weight(mg)=29; MAPavglast3h=45; HRavglast3h=166; QRSAvg46=40; QRSavglast3=37; GA_surg=127; GA_cort=116; GA_sac=141; cutofftime=NaT; gender=m; fetus=<undefined>; breed=17-Dec-2018; cortdate=12-Apr-2019; surgery=23-Apr-2019; oxytocin=NaT; oxytocintime=NaN; sac=07-May-2019 SUBJECT_SAMPLE_FACTORS 1401 1401_L Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/14; Weight(mg)=31.93; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-43472; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=m; fetus=<undefined>; breed=07-Jan-2019; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=0.375; sac=27-May-2019 SUBJECT_SAMPLE_FACTORS 1269 1269_L Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/15; Weight(mg)=27.66; MAPavglast3h=54; HRavglast3h=138; QRSAvg46=40; QRSavglast3=37; GA_surg=128; GA_cort=-; GA_sac=143; cutofftime=05-Apr-2018 09:09:00; gender=m; fetus=s; breed=13-Nov-2017; cortdate=no; surgery=21-Mar-2018; oxytocin=02-Apr-2018; oxytocintime=NaN; sac=05-Apr-2018 SUBJECT_SAMPLE_FACTORS 3022 3022_L Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/38; Weight(mg)=18.67; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=-; GA_sac=142; cutofftime=NaT; gender=f; fetus=<undefined>; breed=24-Sep-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=13-Feb-2019 SUBJECT_SAMPLE_FACTORS 1448 1448_L Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/17; Weight(mg)=38.18; MAPavglast3h=57; HRavglast3h=137; QRSAvg46=44; QRSavglast3=44; GA_surg=123; GA_cort=115; GA_sac=142; cutofftime=18-Apr-2018 16:12:00; gender=m; fetus=s; breed=27-Nov-2017; cortdate=22-Mar-2018; surgery=30-Mar-2018; oxytocin=16-Apr-2018; oxytocintime=NaN; sac=18-Apr-2018 SUBJECT_SAMPLE_FACTORS 1727 1727_L Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/18; Weight(mg)=24; MAPavglast3h=48; HRavglast3h=163; QRSAvg46=52; QRSavglast3=45; GA_surg=127; GA_cort=115; GA_sac=142; cutofftime=28-Mar-2018 08:08:00; gender=f; fetus=s; breed=06-Nov-2017; cortdate=01-Mar-2018; surgery=13-Mar-2018; oxytocin=26-Mar-2018; oxytocintime=NaN; sac=28-Mar-2018 SUBJECT_SAMPLE_FACTORS 1397 1397_L Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/21; Weight(mg)=24.18; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-; GA_cort=-; GA_sac=NaN; cutofftime=NaT; gender=<undefined>; fetus=<undefined>; breed=NaT; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=NaT SUBJECT_SAMPLE_FACTORS 3019 3019_L Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/47; Weight(mg)=23.58; MAPavglast3h=48; HRavglast3h=143; QRSAvg46=41; QRSavglast3=43; GA_surg=NaN; GA_cort=-; GA_sac=141; cutofftime=NaT; gender=f; fetus=<undefined>; breed=10-Sep-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=29-Jan-2019 SUBJECT_SAMPLE_FACTORS 1606 1606_L Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/37; Weight(mg)=26.15; MAPavglast3h=44; HRavglast3h=134; QRSAvg46=29; QRSavglast3=31; GA_surg=126; GA_cort=112; GA_sac=140; cutofftime=NaT; gender=f; fetus=<undefined>; breed=14-Jan-2019; cortdate=06-May-2019; surgery=20-May-2019; oxytocin=NaT; oxytocintime=0.375; sac=03-Jun-2019 SUBJECT_SAMPLE_FACTORS 1775B 1775B_L Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/45; Weight(mg)=30.65; MAPavglast3h=66; HRavglast3h=172; QRSAvg46=NaN; QRSavglast3=36; GA_surg=126; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=<undefined>; fetus=<undefined>; breed=01-Aug-2017; cortdate=no; surgery=05-Dec-2017; oxytocin=17-Dec-2018; oxytocintime=NaN; sac=19-Dec-2017 SUBJECT_SAMPLE_FACTORS 1356 1356_L Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/26; Weight(mg)=26.53; MAPavglast3h=48; HRavglast3h=160; QRSAvg46=37; QRSavglast3=34; GA_surg=127; GA_cort=-; GA_sac=142; cutofftime=04-Apr-2018 08:11:00; gender=m; fetus=s; breed=13-Nov-2017; cortdate=no; surgery=20-Mar-2018; oxytocin=01-Apr-2018; oxytocintime=NaN; sac=04-Apr-2018 SUBJECT_SAMPLE_FACTORS 1445 1445_L Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/27; Weight(mg)=32.16; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=40; QRSavglast3=NaN; GA_surg=127; GA_cort=NaN; GA_sac=141; cutofftime=NaT; gender=f; fetus=s; breed=10-Dec-2018; cortdate=<undefined>; surgery=16-Apr-2019; oxytocin=NaT; oxytocintime=NaN; sac=30-Apr-2019 SUBJECT_SAMPLE_FACTORS 1324 1324_L Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/28; Weight(mg)=40.51; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=-; GA_sac=143; cutofftime=NaT; gender=f; fetus=<undefined>; breed=24-Dec-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=16-May-2019 SUBJECT_SAMPLE_FACTORS 1642 1642_L Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/29; Weight(mg)=41.71; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=NaN; GA_sac=143; cutofftime=NaT; gender=m; fetus=<undefined>; breed=05-Feb-2018; cortdate=<undefined>; surgery=14-Jun-2018; oxytocin=NaT; oxytocintime=NaN; sac=28-Jun-2018 SUBJECT_SAMPLE_FACTORS 1603 1603_L Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/30; Weight(mg)=46.19; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=01-Feb-2018 08:08:00; gender=<undefined>; fetus=s; breed=11-Sep-2017; cortdate=no; surgery=16-Jan-2018; oxytocin=29-Jan-2018; oxytocintime=NaN; sac=01-Feb-2018 SUBJECT_SAMPLE_FACTORS 1277 1277_L Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/31; Weight(mg)=36.29; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=18-Jan-2018 08:10:00; gender=m; fetus=s; breed=28-Aug-2017; cortdate=no; surgery=02-Jan-2018; oxytocin=15-Jan-2018; oxytocintime=NaN; sac=18-Jan-2018 SUBJECT_SAMPLE_FACTORS 1532 1532_L Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/32; Weight(mg)=32.83; MAPavglast3h=57; HRavglast3h=149; QRSAvg46=41; QRSavglast3=41; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=15-Mar-2018 08:13:00; gender=f; fetus=s; breed=23-Oct-2017; cortdate=no; surgery=27-Feb-2018; oxytocin=12-Mar-2018; oxytocintime=NaN; sac=15-Mar-2018 SUBJECT_SAMPLE_FACTORS 1656 1656_L Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_L_03172020/33; Weight(mg)=35.07; MAPavglast3h=63; HRavglast3h=159; QRSAvg46=NaN; QRSavglast3=37; GA_surg=133; GA_cort=-; GA_sac=141; cutofftime=07-Nov-2017 13:44:00; gender=f; fetus=t; breed=19-Jun-2017; cortdate=no; surgery=30-Oct-2017; oxytocin=05-Nov-2017; oxytocintime=NaN; sac=07-Nov-2017 SUBJECT_SAMPLE_FACTORS 1682 1682_R Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/41; Weight(mg)=25.45; MAPavglast3h=NaN; HRavglast3h=137; QRSAvg46=34; QRSavglast3=38; GA_surg=-43150; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=m; fetus=<undefined>; breed=19-Feb-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=09-Jul-2018 SUBJECT_SAMPLE_FACTORS 1428 1428_R Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/42; Weight(mg)=28.41; MAPavglast3h=49; HRavglast3h=150; QRSAvg46=41; QRSavglast3=48; GA_surg=128; GA_cort=116; GA_sac=143; cutofftime=26-Apr-2018 09:15:00; gender=m; fetus=s; breed=04-Dec-2017; cortdate=30-Mar-2018; surgery=11-Apr-2018; oxytocin=23-Apr-2018; oxytocintime=NaN; sac=26-Apr-2018 SUBJECT_SAMPLE_FACTORS 1775 1775_R Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/47; Weight(mg)=36.61; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=126; GA_cort=-; GA_sac=140; cutofftime=19-Dec-2017 08:09:00; gender=f; fetus=triplet; breed=01-Aug-2017; cortdate=no; surgery=05-Dec-2017; oxytocin=17-Dec-2018; oxytocintime=NaN; sac=19-Dec-2017 SUBJECT_SAMPLE_FACTORS 1427 1427_R Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/43; Weight(mg)=28.67; MAPavglast3h=52; HRavglast3h=129; QRSAvg46=41; QRSavglast3=45; GA_surg=123; GA_cort=-; GA_sac=142; cutofftime=NaT; gender=m; fetus=<undefined>; breed=03-Sep-2018; cortdate=no; surgery=04-Jan-2019; oxytocin=NaT; oxytocintime=NaN; sac=23-Jan-2019 SUBJECT_SAMPLE_FACTORS 1329 1329_R Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/49; Weight(mg)=32.57; MAPavglast3h=55; HRavglast3h=164; QRSAvg46=NaN; QRSavglast3=46; GA_surg=122; GA_cort=115; GA_sac=141; cutofftime=NaT; gender=m; fetus=<undefined>; breed=24-Sep-2018; cortdate=17-Jan-2019; surgery=24-Jan-2019; oxytocin=NaT; oxytocintime=NaN; sac=12-Feb-2019 SUBJECT_SAMPLE_FACTORS 1261 1261_R Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/50; Weight(mg)=29.35; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=116; GA_sac=142; cutofftime=NaT; gender=m; fetus=s; breed=25-Sep-2017; cortdate=19-Jan-2018; surgery=30-Jan-2018; oxytocin=11-Feb-2018; oxytocintime=NaN; sac=14-Feb-2018 SUBJECT_SAMPLE_FACTORS 1668 1668_R Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/52; Weight(mg)=33.77; MAPavglast3h=56; HRavglast3h=139; QRSAvg46=36; QRSavglast3=38; GA_surg=120; GA_cort=115; GA_sac=140; cutofftime=NaT; gender=f; fetus=<undefined>; breed=25-Jun-2018; cortdate=18-Oct-2018; surgery=23-Oct-2018; oxytocin=NaT; oxytocintime=NaN; sac=12-Nov-2018 SUBJECT_SAMPLE_FACTORS 1611 1611_R Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_test_SZ_R_06242020/1; Weight(mg)=27.94; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=130; GA_cort=-; GA_sac=144; cutofftime=NaT; gender=f; fetus=s; breed=15-Jan-2018; cortdate=no; surgery=25-May-2018; oxytocin=05-Jun-2018; oxytocintime=NaN; sac=08-Jun-2018 SUBJECT_SAMPLE_FACTORS 1254 1254_R Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/16; Weight(mg)=37.24; MAPavglast3h=56; HRavglast3h=139; QRSAvg46=37; QRSavglast3=37; GA_surg=123; GA_cort=-; GA_sac=142; cutofftime=01-Nov-2017 07:47:00; gender=m; fetus=s; breed=12-Jun-2017; cortdate=no; surgery=13-Oct-2017; oxytocin=29-Oct-2017; oxytocintime=NaN; sac=01-Nov-2017 SUBJECT_SAMPLE_FACTORS 1565 1565_R Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/18; Weight(mg)=36.98; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-43458; GA_cort=-43458; GA_sac=147; cutofftime=NaT; gender=m; fetus=<undefined>; breed=24-Dec-2018; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=20-May-2019 SUBJECT_SAMPLE_FACTORS 1719 1719_R Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/19; Weight(mg)=25.24; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-; GA_cort=-; GA_sac=NaN; cutofftime=NaT; gender=m; fetus=<undefined>; breed=NaT; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=0.3125; sac=NaT SUBJECT_SAMPLE_FACTORS 1212 1212_R Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/20; Weight(mg)=28.3; MAPavglast3h=45; HRavglast3h=166; QRSAvg46=40; QRSavglast3=37; GA_surg=127; GA_cort=116; GA_sac=141; cutofftime=NaT; gender=m; fetus=<undefined>; breed=17-Dec-2018; cortdate=12-Apr-2019; surgery=23-Apr-2019; oxytocin=NaT; oxytocintime=NaN; sac=07-May-2019 SUBJECT_SAMPLE_FACTORS 1401 1401_R Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/21; Weight(mg)=37.14; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-43472; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=m; fetus=<undefined>; breed=07-Jan-2019; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=0.375; sac=27-May-2019 SUBJECT_SAMPLE_FACTORS 1269 1269_R Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/22; Weight(mg)=32.17; MAPavglast3h=54; HRavglast3h=138; QRSAvg46=40; QRSavglast3=37; GA_surg=128; GA_cort=-; GA_sac=143; cutofftime=05-Apr-2018 09:09:00; gender=m; fetus=s; breed=13-Nov-2017; cortdate=no; surgery=21-Mar-2018; oxytocin=02-Apr-2018; oxytocintime=NaN; sac=05-Apr-2018 SUBJECT_SAMPLE_FACTORS 3022 3022_R Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/23; Weight(mg)=27.71; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=-; GA_sac=142; cutofftime=NaT; gender=f; fetus=<undefined>; breed=24-Sep-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=13-Feb-2019 SUBJECT_SAMPLE_FACTORS 1448 1448_R Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/24; Weight(mg)=26.72; MAPavglast3h=57; HRavglast3h=137; QRSAvg46=44; QRSavglast3=44; GA_surg=123; GA_cort=115; GA_sac=142; cutofftime=18-Apr-2018 16:12:00; gender=m; fetus=s; breed=27-Nov-2017; cortdate=22-Mar-2018; surgery=30-Mar-2018; oxytocin=16-Apr-2018; oxytocintime=NaN; sac=18-Apr-2018 SUBJECT_SAMPLE_FACTORS 1727 1727_R Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/25; Weight(mg)=32.58; MAPavglast3h=48; HRavglast3h=163; QRSAvg46=52; QRSavglast3=45; GA_surg=127; GA_cort=115; GA_sac=142; cutofftime=28-Mar-2018 08:08:00; gender=f; fetus=s; breed=06-Nov-2017; cortdate=01-Mar-2018; surgery=13-Mar-2018; oxytocin=26-Mar-2018; oxytocintime=NaN; sac=28-Mar-2018 SUBJECT_SAMPLE_FACTORS 1397 1397_R Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/26; Weight(mg)=35.43; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-; GA_cort=-; GA_sac=NaN; cutofftime=NaT; gender=<undefined>; fetus=<undefined>; breed=NaT; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=NaT SUBJECT_SAMPLE_FACTORS 3019 3019_R Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/28; Weight(mg)=40.04; MAPavglast3h=48; HRavglast3h=143; QRSAvg46=41; QRSavglast3=43; GA_surg=NaN; GA_cort=-; GA_sac=141; cutofftime=NaT; gender=f; fetus=<undefined>; breed=10-Sep-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=29-Jan-2019 SUBJECT_SAMPLE_FACTORS 1606 1606_R Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/29; Weight(mg)=36.34; MAPavglast3h=44; HRavglast3h=134; QRSAvg46=29; QRSavglast3=31; GA_surg=126; GA_cort=112; GA_sac=140; cutofftime=NaT; gender=f; fetus=<undefined>; breed=14-Jan-2019; cortdate=06-May-2019; surgery=20-May-2019; oxytocin=NaT; oxytocintime=0.375; sac=03-Jun-2019 SUBJECT_SAMPLE_FACTORS 1775B 1775B_R Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/30; Weight(mg)=33.61; MAPavglast3h=66; HRavglast3h=172; QRSAvg46=NaN; QRSavglast3=36; GA_surg=126; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=<undefined>; fetus=<undefined>; breed=01-Aug-2017; cortdate=no; surgery=05-Dec-2017; oxytocin=17-Dec-2018; oxytocintime=NaN; sac=19-Dec-2017 SUBJECT_SAMPLE_FACTORS 1356 1356_R Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/31; Weight(mg)=25.96; MAPavglast3h=48; HRavglast3h=160; QRSAvg46=37; QRSavglast3=34; GA_surg=127; GA_cort=-; GA_sac=142; cutofftime=04-Apr-2018 08:11:00; gender=m; fetus=s; breed=13-Nov-2017; cortdate=no; surgery=20-Mar-2018; oxytocin=01-Apr-2018; oxytocintime=NaN; sac=04-Apr-2018 SUBJECT_SAMPLE_FACTORS 1445 1445_R Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/32; Weight(mg)=35.29; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=40; QRSavglast3=NaN; GA_surg=127; GA_cort=NaN; GA_sac=141; cutofftime=NaT; gender=f; fetus=s; breed=10-Dec-2018; cortdate=<undefined>; surgery=16-Apr-2019; oxytocin=NaT; oxytocintime=NaN; sac=30-Apr-2019 SUBJECT_SAMPLE_FACTORS 1324 1324_R Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/33; Weight(mg)=34.31; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=-; GA_sac=143; cutofftime=NaT; gender=f; fetus=<undefined>; breed=24-Dec-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=16-May-2019 SUBJECT_SAMPLE_FACTORS 1642 1642_R Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/34; Weight(mg)=36.25; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=NaN; GA_sac=143; cutofftime=NaT; gender=m; fetus=<undefined>; breed=05-Feb-2018; cortdate=<undefined>; surgery=14-Jun-2018; oxytocin=NaT; oxytocintime=NaN; sac=28-Jun-2018 SUBJECT_SAMPLE_FACTORS 1603 1603_R Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/35; Weight(mg)=30.37; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=01-Feb-2018 08:08:00; gender=<undefined>; fetus=s; breed=11-Sep-2017; cortdate=no; surgery=16-Jan-2018; oxytocin=29-Jan-2018; oxytocintime=NaN; sac=01-Feb-2018 SUBJECT_SAMPLE_FACTORS 1277 1277_R Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/38; Weight(mg)=38.22; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=18-Jan-2018 08:10:00; gender=m; fetus=s; breed=28-Aug-2017; cortdate=no; surgery=02-Jan-2018; oxytocin=15-Jan-2018; oxytocintime=NaN; sac=18-Jan-2018 SUBJECT_SAMPLE_FACTORS 1532 1532_R Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/39; Weight(mg)=37.77; MAPavglast3h=57; HRavglast3h=149; QRSAvg46=41; QRSavglast3=41; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=15-Mar-2018 08:13:00; gender=f; fetus=s; breed=23-Oct-2017; cortdate=no; surgery=27-Feb-2018; oxytocin=12-Mar-2018; oxytocintime=NaN; sac=15-Mar-2018 SUBJECT_SAMPLE_FACTORS 1656 1656_R Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_R_06222020/40; Weight(mg)=36.65; MAPavglast3h=63; HRavglast3h=159; QRSAvg46=NaN; QRSavglast3=37; GA_surg=133; GA_cort=-; GA_sac=141; cutofftime=07-Nov-2017 13:44:00; gender=f; fetus=t; breed=19-Jun-2017; cortdate=no; surgery=30-Oct-2017; oxytocin=05-Nov-2017; oxytocintime=NaN; sac=07-Nov-2017 SUBJECT_SAMPLE_FACTORS 1682 1682_S Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/2; Weight(mg)=34.02; MAPavglast3h=NaN; HRavglast3h=137; QRSAvg46=34; QRSavglast3=38; GA_surg=-43150; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=m; fetus=<undefined>; breed=19-Feb-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=09-Jul-2018 SUBJECT_SAMPLE_FACTORS 1428 1428_S Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/3; Weight(mg)=26.89; MAPavglast3h=49; HRavglast3h=150; QRSAvg46=41; QRSavglast3=48; GA_surg=128; GA_cort=116; GA_sac=143; cutofftime=26-Apr-2018 09:15:00; gender=m; fetus=s; breed=04-Dec-2017; cortdate=30-Mar-2018; surgery=11-Apr-2018; oxytocin=23-Apr-2018; oxytocintime=NaN; sac=26-Apr-2018 SUBJECT_SAMPLE_FACTORS 1775 1775_S Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/4; Weight(mg)=29.54; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=126; GA_cort=-; GA_sac=140; cutofftime=19-Dec-2017 08:09:00; gender=f; fetus=triplet; breed=01-Aug-2017; cortdate=no; surgery=05-Dec-2017; oxytocin=17-Dec-2018; oxytocintime=NaN; sac=19-Dec-2017 SUBJECT_SAMPLE_FACTORS 1427 1427_S Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/5; Weight(mg)=38.24; MAPavglast3h=52; HRavglast3h=129; QRSAvg46=41; QRSavglast3=45; GA_surg=123; GA_cort=-; GA_sac=142; cutofftime=NaT; gender=m; fetus=<undefined>; breed=03-Sep-2018; cortdate=no; surgery=04-Jan-2019; oxytocin=NaT; oxytocintime=NaN; sac=23-Jan-2019 SUBJECT_SAMPLE_FACTORS 1329 1329_S Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/6; Weight(mg)=31.7; MAPavglast3h=55; HRavglast3h=164; QRSAvg46=NaN; QRSavglast3=46; GA_surg=122; GA_cort=115; GA_sac=141; cutofftime=NaT; gender=m; fetus=<undefined>; breed=24-Sep-2018; cortdate=17-Jan-2019; surgery=24-Jan-2019; oxytocin=NaT; oxytocintime=NaN; sac=12-Feb-2019 SUBJECT_SAMPLE_FACTORS 1261 1261_S Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/8; Weight(mg)=43.65; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=116; GA_sac=142; cutofftime=NaT; gender=m; fetus=s; breed=25-Sep-2017; cortdate=19-Jan-2018; surgery=30-Jan-2018; oxytocin=11-Feb-2018; oxytocintime=NaN; sac=14-Feb-2018 SUBJECT_SAMPLE_FACTORS 1668 1668_S Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/9; Weight(mg)=40.14; MAPavglast3h=56; HRavglast3h=139; QRSAvg46=36; QRSavglast3=38; GA_surg=120; GA_cort=115; GA_sac=140; cutofftime=NaT; gender=f; fetus=<undefined>; breed=25-Jun-2018; cortdate=18-Oct-2018; surgery=23-Oct-2018; oxytocin=NaT; oxytocintime=NaN; sac=12-Nov-2018 SUBJECT_SAMPLE_FACTORS 1611 1611_S Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/10; Weight(mg)=44.28; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=130; GA_cort=-; GA_sac=144; cutofftime=NaT; gender=f; fetus=s; breed=15-Jan-2018; cortdate=no; surgery=25-May-2018; oxytocin=05-Jun-2018; oxytocintime=NaN; sac=08-Jun-2018 SUBJECT_SAMPLE_FACTORS 1254 1254_S Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/11; Weight(mg)=39.48; MAPavglast3h=56; HRavglast3h=139; QRSAvg46=37; QRSavglast3=37; GA_surg=123; GA_cort=-; GA_sac=142; cutofftime=01-Nov-2017 07:47:00; gender=m; fetus=s; breed=12-Jun-2017; cortdate=no; surgery=13-Oct-2017; oxytocin=29-Oct-2017; oxytocintime=NaN; sac=01-Nov-2017 SUBJECT_SAMPLE_FACTORS 1565 1565_S Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/12; Weight(mg)=34.15; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-43458; GA_cort=-43458; GA_sac=147; cutofftime=NaT; gender=m; fetus=<undefined>; breed=24-Dec-2018; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=20-May-2019 SUBJECT_SAMPLE_FACTORS 1719 1719_S Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/13; Weight(mg)=31.81; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-; GA_cort=-; GA_sac=NaN; cutofftime=NaT; gender=m; fetus=<undefined>; breed=NaT; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=0.3125; sac=NaT SUBJECT_SAMPLE_FACTORS 1212 1212_S Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/14; Weight(mg)=36.17; MAPavglast3h=45; HRavglast3h=166; QRSAvg46=40; QRSavglast3=37; GA_surg=127; GA_cort=116; GA_sac=141; cutofftime=NaT; gender=m; fetus=<undefined>; breed=17-Dec-2018; cortdate=12-Apr-2019; surgery=23-Apr-2019; oxytocin=NaT; oxytocintime=NaN; sac=07-May-2019 SUBJECT_SAMPLE_FACTORS 1401 1401_S Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/15; Weight(mg)=30.83; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-43472; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=m; fetus=<undefined>; breed=07-Jan-2019; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=0.375; sac=27-May-2019 SUBJECT_SAMPLE_FACTORS 1269 1269_S Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/17; Weight(mg)=35.45; MAPavglast3h=54; HRavglast3h=138; QRSAvg46=40; QRSavglast3=37; GA_surg=128; GA_cort=-; GA_sac=143; cutofftime=05-Apr-2018 09:09:00; gender=m; fetus=s; breed=13-Nov-2017; cortdate=no; surgery=21-Mar-2018; oxytocin=02-Apr-2018; oxytocintime=NaN; sac=05-Apr-2018 SUBJECT_SAMPLE_FACTORS 3022 3022_S Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/18; Weight(mg)=26.2; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=-; GA_sac=142; cutofftime=NaT; gender=f; fetus=<undefined>; breed=24-Sep-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=13-Feb-2019 SUBJECT_SAMPLE_FACTORS 1448 1448_S Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/19; Weight(mg)=28.16; MAPavglast3h=57; HRavglast3h=137; QRSAvg46=44; QRSavglast3=44; GA_surg=123; GA_cort=115; GA_sac=142; cutofftime=18-Apr-2018 16:12:00; gender=m; fetus=s; breed=27-Nov-2017; cortdate=22-Mar-2018; surgery=30-Mar-2018; oxytocin=16-Apr-2018; oxytocintime=NaN; sac=18-Apr-2018 SUBJECT_SAMPLE_FACTORS 1727 1727_S Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/20; Weight(mg)=28.43; MAPavglast3h=48; HRavglast3h=163; QRSAvg46=52; QRSavglast3=45; GA_surg=127; GA_cort=115; GA_sac=142; cutofftime=28-Mar-2018 08:08:00; gender=f; fetus=s; breed=06-Nov-2017; cortdate=01-Mar-2018; surgery=13-Mar-2018; oxytocin=26-Mar-2018; oxytocintime=NaN; sac=28-Mar-2018 SUBJECT_SAMPLE_FACTORS 1397 1397_S Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/21; Weight(mg)=31.9; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=-; GA_cort=-; GA_sac=NaN; cutofftime=NaT; gender=<undefined>; fetus=<undefined>; breed=NaT; cortdate=<undefined>; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=NaT SUBJECT_SAMPLE_FACTORS 3019 3019_S Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/22; Weight(mg)=31.46; MAPavglast3h=48; HRavglast3h=143; QRSAvg46=41; QRSavglast3=43; GA_surg=NaN; GA_cort=-; GA_sac=141; cutofftime=NaT; gender=f; fetus=<undefined>; breed=10-Sep-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=29-Jan-2019 SUBJECT_SAMPLE_FACTORS 1606 1606_S Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/23; Weight(mg)=28.72; MAPavglast3h=44; HRavglast3h=134; QRSAvg46=29; QRSavglast3=31; GA_surg=126; GA_cort=112; GA_sac=140; cutofftime=NaT; gender=f; fetus=<undefined>; breed=14-Jan-2019; cortdate=06-May-2019; surgery=20-May-2019; oxytocin=NaT; oxytocintime=0.375; sac=03-Jun-2019 SUBJECT_SAMPLE_FACTORS 1775B 1775B_S Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/24; Weight(mg)=45.09; MAPavglast3h=66; HRavglast3h=172; QRSAvg46=NaN; QRSavglast3=36; GA_surg=126; GA_cort=-; GA_sac=140; cutofftime=NaT; gender=<undefined>; fetus=<undefined>; breed=01-Aug-2017; cortdate=no; surgery=05-Dec-2017; oxytocin=17-Dec-2018; oxytocintime=NaN; sac=19-Dec-2017 SUBJECT_SAMPLE_FACTORS 1356 1356_S Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/25; Weight(mg)=34.64; MAPavglast3h=48; HRavglast3h=160; QRSAvg46=37; QRSavglast3=34; GA_surg=127; GA_cort=-; GA_sac=142; cutofftime=04-Apr-2018 08:11:00; gender=m; fetus=s; breed=13-Nov-2017; cortdate=no; surgery=20-Mar-2018; oxytocin=01-Apr-2018; oxytocintime=NaN; sac=04-Apr-2018 SUBJECT_SAMPLE_FACTORS 1445 1445_S Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/27; Weight(mg)=36.72; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=40; QRSavglast3=NaN; GA_surg=127; GA_cort=NaN; GA_sac=141; cutofftime=NaT; gender=f; fetus=s; breed=10-Dec-2018; cortdate=<undefined>; surgery=16-Apr-2019; oxytocin=NaT; oxytocintime=NaN; sac=30-Apr-2019 SUBJECT_SAMPLE_FACTORS 1324 1324_S Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/28; Weight(mg)=42.25; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=-; GA_sac=143; cutofftime=NaT; gender=f; fetus=<undefined>; breed=24-Dec-2018; cortdate=no; surgery=NaT; oxytocin=NaT; oxytocintime=NaN; sac=16-May-2019 SUBJECT_SAMPLE_FACTORS 1642 1642_S Cortisol treatment:Cortisol-infused (1 g/kg/d from 115 to term) | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/29; Weight(mg)=36.16; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=NaN; GA_cort=NaN; GA_sac=143; cutofftime=NaT; gender=m; fetus=<undefined>; breed=05-Feb-2018; cortdate=<undefined>; surgery=14-Jun-2018; oxytocin=NaT; oxytocintime=NaN; sac=28-Jun-2018 SUBJECT_SAMPLE_FACTORS 1603 1603_S Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/30; Weight(mg)=45.64; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=01-Feb-2018 08:08:00; gender=<undefined>; fetus=s; breed=11-Sep-2017; cortdate=no; surgery=16-Jan-2018; oxytocin=29-Jan-2018; oxytocintime=NaN; sac=01-Feb-2018 SUBJECT_SAMPLE_FACTORS 1277 1277_S Cortisol treatment:Control | DCA treatment:DCA-treated (over 24h) RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/31; Weight(mg)=28.17; MAPavglast3h=NaN; HRavglast3h=NaN; QRSAvg46=NaN; QRSavglast3=NaN; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=18-Jan-2018 08:10:00; gender=m; fetus=s; breed=28-Aug-2017; cortdate=no; surgery=02-Jan-2018; oxytocin=15-Jan-2018; oxytocintime=NaN; sac=18-Jan-2018 SUBJECT_SAMPLE_FACTORS 1532 1532_S Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/32; Weight(mg)=28.29; MAPavglast3h=57; HRavglast3h=149; QRSAvg46=41; QRSavglast3=41; GA_surg=127; GA_cort=-; GA_sac=143; cutofftime=15-Mar-2018 08:13:00; gender=f; fetus=s; breed=23-Oct-2017; cortdate=no; surgery=27-Feb-2018; oxytocin=12-Mar-2018; oxytocintime=NaN; sac=15-Mar-2018 SUBJECT_SAMPLE_FACTORS 1656 1656_S Cortisol treatment:Control | DCA treatment:Control RAW_FILE_NAME=Sheep_heart_noesy_SZ_S_06302020/33; Weight(mg)=29.92; MAPavglast3h=63; HRavglast3h=159; QRSAvg46=NaN; QRSavglast3=37; GA_surg=133; GA_cort=-; GA_sac=141; cutofftime=07-Nov-2017 13:44:00; gender=f; fetus=t; breed=19-Jun-2017; cortdate=no; surgery=30-Oct-2017; oxytocin=05-Nov-2017; oxytocintime=NaN; sac=07-Nov-2017 #COLLECTION CO:COLLECTION_SUMMARY Heart tissue was collected from the right ventricle, left ventricle, and CO:COLLECTION_SUMMARY intraventricular septum and immediately frozen in liquid nitrogen. CO:SAMPLE_TYPE Heart CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY Animals in the CORT and CORT+DCA groups were implanted with pellets of cortisol TR:TREATMENT_SUMMARY hemisuccinate (Innovative Research, Sarasota, FL) or infused with cortisol TR:TREATMENT_SUMMARY hemisuccinate (Solu-Cortef, Pfizer, Inc) using an ambulatory pump (3D Micro TR:TREATMENT_SUMMARY Infusion Pump; Strategic Applications Inc.; Lake Villa, IL) to provide dosing of TR:TREATMENT_SUMMARY cortisol at 1mg/kg/day beginning on day 115-116 of gestation until the end of TR:TREATMENT_SUMMARY the experiment when the animals were sacrificed. This dose of cortisol increased TR:TREATMENT_SUMMARY maternal cortisol concentrations to approximately 1.5-fold, mimicking the rise TR:TREATMENT_SUMMARY in cortisol in moderate stress. To produce a regular pattern of labor, ewes were TR:TREATMENT_SUMMARY infused with oxytocin (Aspen Veterinary Resources) beginning at day 138-140 of TR:TREATMENT_SUMMARY gestation; oxytocin was infused over 5 minutes every 30 minutes (820 µU/kg/min TR:TREATMENT_SUMMARY iv;) using an infusion pump controlled by a timed controller ChronTrol Corp, TR:TREATMENT_SUMMARY Inc). DCA infusion was started 48 hours after the start of oxytocin. DCA was TR:TREATMENT_SUMMARY administered as an intravenous bolus of 25 mg/kg over 3 minutes, followed by an TR:TREATMENT_SUMMARY infusion of 12.5 mg DCA/kg/hour for 8 hours, and an infusion of 6.25 mg DCA/kg/h TR:TREATMENT_SUMMARY for the next 8 hours. These doses are similar to those that significantly TR:TREATMENT_SUMMARY decrease circulating lactate concentrations in humans. The timed infusions of TR:TREATMENT_SUMMARY oxytocin continued for 72 hours, and the study was terminated after 24 hours of TR:TREATMENT_SUMMARY DCA and/or 72 hours of oxytocin infusion, unless ewes were in the process of TR:TREATMENT_SUMMARY delivery or birth occurred. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Heart tissues were cut to ~ 30 mg and weighted. Each tissue was added to 30 µl SP:SAMPLEPREP_SUMMARY of D2O with 10/3 mM sodium trimethylsilylpropane-sulfonate (DSS) and transferred SP:SAMPLEPREP_SUMMARY to a 4-mm zirconium dioxide rotor with Kel-F cap and PTFE spacer provided by SP:SAMPLEPREP_SUMMARY Bruker Biospin. Samples were kept on dry ice during sample preparation. SP:SAMPLEPREP_PROTOCOL_FILENAME SamplePrepProtocol.pdf #ANALYSIS AN:ANALYSIS_TYPE NMR AN:LABORATORY_NAME Edison Lab AN:SOFTWARE_VERSION Topspin 4.0.6 #NMR NM:INSTRUMENT_NAME Bruker NEO 600MHz NM:INSTRUMENT_TYPE FT-NMR NM:NMR_EXPERIMENT_TYPE 1D-1H NM:FIELD_FREQUENCY_LOCK D2O NM:SPECTROMETER_FREQUENCY 600 MHz NM:NMR_PROBE 4mm CMP_HRMAS NM:NMR_SOLVENT D2O NM:NMR_TUBE_SIZE 50 ul NM:SHIMMING_METHOD manual NM:PULSE_SEQUENCE noesypr1d NM:WATER_SUPPRESSION presat NM:RECEIVER_GAIN 101 NM:CHEMICAL_SHIFT_REF_CPD DSS NM:TEMPERATURE 10 NM:NUMBER_OF_SCANS 128 NM:DUMMY_SCANS 8 NM:ACQUISITION_TIME 1.70 s NM:SPECTRAL_WIDTH 16.0240 ppm NM:NUM_DATA_POINTS_ACQUIRED 32768 NM:LINE_BROADENING 1.8 Hz NM:BASELINE_CORRECTION_METHOD ABSG NM:NMR_RESULTS_FILE Noesy_binned_data.txt UNITS:(relative) #END