#METABOLOMICS WORKBENCH Andressa_Stuart_20211112_035200 DATATRACK_ID:2926 STUDY_ID:ST001990 ANALYSIS_ID:AN003243 VERSION 1 CREATED_ON 02-08-2024 #PROJECT PR:PROJECT_TITLE Metabolomics of the interaction between a consortium of entomopathogenic fungi PR:PROJECT_TITLE and their target insect: mechanisms of attack and survival PR:PROJECT_TYPE Untargeted Metabolomics PR:PROJECT_SUMMARY One of the most concerning pests that attack strawberries in Brazil is PR:PROJECT_SUMMARY Duponchelia fovealis, a non-native moth with no registered control methods to PR:PROJECT_SUMMARY date. Our group recently observed that a fungal consortium formed by two strains PR:PROJECT_SUMMARY of Beauveria bassiana increased the mortality of D. fovealis more than PR:PROJECT_SUMMARY inoculation with each strain on its own. However, the molecular interaction PR:PROJECT_SUMMARY between the fungal consortium and the caterpillars is unknown, raising several PR:PROJECT_SUMMARY questions about the enhanced pest control observed. Furthermore, concerns over PR:PROJECT_SUMMARY the emergency of resistance and the selection for resistance to chemical and PR:PROJECT_SUMMARY biological products that are constantly applied in agriculture highlight the PR:PROJECT_SUMMARY need for careful examination of novel pest control methods. Thus, in this work, PR:PROJECT_SUMMARY we sought to pioneer the evaluation of the molecular interaction between a PR:PROJECT_SUMMARY fungal consortium of B. bassiana and D. fovealis caterpillars. We aimed to PR:PROJECT_SUMMARY understand the biocontrol process involved in this interaction and the defense PR:PROJECT_SUMMARY system of the caterpillar. Therefore, seven days after D. fovealis caterpillars PR:PROJECT_SUMMARY were inoculated with the B. bassiana consortium, the dead and surviving PR:PROJECT_SUMMARY caterpillars were analyzed using GC-MS and LC-MS/MS. PR:INSTITUTE Universidade Federal do Paraná PR:DEPARTMENT Patologia Básica PR:LABORATORY Laboratório de Microbiologia e Biologia Molecular PR:LAST_NAME Katiski da Costa Stuart PR:FIRST_NAME Andressa PR:ADDRESS Av. Cel. Francisco Heráclito dos Santos, 100, Curitiba, Paraná, 81530-000, PR:ADDRESS Brazil PR:EMAIL andressa.katiski@gmail.com PR:PHONE 5541991922779 PR:PROJECT_COMMENTS Previous studies by our research group showed the increased potential for PR:PROJECT_COMMENTS biocontrol on the insect pest Duponchelia fovealis when two strains of Beauveria PR:PROJECT_COMMENTS bassiana were used together as a fungal consortium. In this work we sought to PR:PROJECT_COMMENTS identify the metabolites involved in the interaction between the consortium PR:PROJECT_COMMENTS formed by different B. bassiana strains and its target insect, D. fovealis. We PR:PROJECT_COMMENTS identify the metabolites using non-targeted metabolomics, applying gas and PR:PROJECT_COMMENTS liquid chromatography coupled to mass spectrometers (GC-MS and LC-MS/MS). These PR:PROJECT_COMMENTS analyses aimed to elucidate the molecular mechanisms involved in the biocontrol PR:PROJECT_COMMENTS effect of the fungal consortium on D. fovealis and to examine possible PR:PROJECT_COMMENTS explanations for the survival of some caterpillars due to a potential resistance PR:PROJECT_COMMENTS mechanism. Dead and surviving caterpillars were analyzed separately. PR:DOI http://dx.doi.org/10.21228/M8B39F PR:CONTRIBUTORS Jason Lee Furuie, Thais Regiani Cataldi, Rodrigo Makowiecky Stuart, Maria PR:CONTRIBUTORS Aparecida Cassilha Zawadneak, Carlos Alberto Labate, Ida Chapaval Pimentel #STUDY ST:STUDY_TITLE Metabolomics of the interaction between a consortium of entomopathogenic fungi ST:STUDY_TITLE and their target insect: mechanisms of attack and survival ST:STUDY_TYPE Untargeted Metabolomics ST:STUDY_SUMMARY One of the most concerning pests that attack strawberries in Brazil is ST:STUDY_SUMMARY Duponchelia fovealis, a non-native moth with no registered control methods to ST:STUDY_SUMMARY date. Our group recently observed that a fungal consortium formed by two strains ST:STUDY_SUMMARY of Beauveria bassiana increased the mortality of D. fovealis more than ST:STUDY_SUMMARY inoculation with each strain on its own. However, the molecular interaction ST:STUDY_SUMMARY between the fungal consortium and the caterpillars is unknown, raising several ST:STUDY_SUMMARY questions about the enhanced pest control observed. Furthermore, concerns over ST:STUDY_SUMMARY the emergency of resistance and the selection for resistance to chemical and ST:STUDY_SUMMARY biological products that are constantly applied in agriculture highlight the ST:STUDY_SUMMARY need for careful examination of novel pest control methods. Thus, in this work, ST:STUDY_SUMMARY we sought to pioneer the evaluation of the molecular interaction between a ST:STUDY_SUMMARY fungal consortium of B. bassiana and D. fovealis caterpillars. We aimed to ST:STUDY_SUMMARY understand the biocontrol process involved in this interaction and the defense ST:STUDY_SUMMARY system of the caterpillar. Therefore, seven days after D. fovealis caterpillars ST:STUDY_SUMMARY were inoculated with the B. bassiana consortium, the dead and surviving ST:STUDY_SUMMARY caterpillars were analyzed using GC-MS and LC-MS/MS. ST:INSTITUTE Universidade Federal do Paraná ST:DEPARTMENT Patologia Básica ST:LABORATORY Laboratório de Microbiologia e Biologia Molecular ST:LAST_NAME Katiski da Costa Stuart ST:FIRST_NAME Andressa ST:ADDRESS Av. Cel. Francisco Heráclito dos Santos, 100, Curitiba, Paraná, 81530-000, ST:ADDRESS Brazil ST:EMAIL andressa.katiski@gmail.com ST:PHONE 55 41 991922779 ST:SUBMIT_DATE 2021-11-12 #SUBJECT SU:SUBJECT_TYPE Insect SU:SUBJECT_SPECIES Duponchelia fovealis #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - CatS_1 Factor:Control RAW_FILE_NAME=CatS_1; RAW_FILE_NAME=CatS_1_R001 CatS_1_R002 CatS_1_R003.; RAW_FILE_NAME=CatS_1_R001_NEG CatS_1_R002_NEG CatS_1_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_2 Factor:Control RAW_FILE_NAME=CatS_2; RAW_FILE_NAME=CatS_2_R001 CatS_2_R002 CatS_2_R003.; RAW_FILE_NAME=CatS_2_R001_NEG CatS_2_R002_NEG CatS_2_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_3 Factor:Control RAW_FILE_NAME=CatS_3; RAW_FILE_NAME=CatS_3_R001 CatS_3_R002 CatS_3_R003.; RAW_FILE_NAME=CatS_3_R001_NEG CatS_3_R002_NEG CatS_3_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_4 Factor:Control RAW_FILE_NAME=CatS_4; RAW_FILE_NAME=CatS_4_R001 CatS_4_R002 CatS_4_R003.; RAW_FILE_NAME=CatS_4_R001_NEG CatS_4_R002_NEG CatS_4_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_5 Factor:Control RAW_FILE_NAME=CatS_5; RAW_FILE_NAME=CatS_5_R001 CatS_5_R002 CatS_5_R003.; RAW_FILE_NAME=CatS_5_R001_NEG CatS_5_R002_NEG CatS_5_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatM_Bov2_1 Factor:Duponchelia fovealis killed by the Bov2 strain of Beauveria bassiana RAW_FILE_NAME=CatM_Bov2_1; RAW_FILE_NAME=CatM_Bov2_1_R001 CatM_Bov2_1_R002 CatM_Bov2_1_R003 CatM_Bov2_1_R004.; RAW_FILE_NAME=CatM_Bov2_1_R001_NEG CatM_Bov2_1_R002_NEG CatM_Bov2_1_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatM_Bov2_2 Factor:Duponchelia fovealis killed by the Bov2 strain of Beauveria bassiana RAW_FILE_NAME=CatM_Bov2_2; RAW_FILE_NAME=CatM_Bov2_2_R001 CatM_Bov2_2_R002 CatM_Bov2_2_R003 CatM_Bov2_2_R004.; RAW_FILE_NAME=CatM_Bov2_2_R001_NEG CatM_Bov2_2_R002_NEG CatM_Bov2_2_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatM_Bov2_3 Factor:Duponchelia fovealis killed by the Bov2 strain of Beauveria bassiana RAW_FILE_NAME=CatM_Bov2_3; RAW_FILE_NAME=CatM_Bov2_3_R001 CatM_Bov2_3_R002 CatM_Bov2_3_R003 CatM_Bov2_3_R004.; RAW_FILE_NAME=CatM_Bov2_3_R001_NEG CatM_Bov2_3_R002_NEG CatM_Bov2_3_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatM_Bov2_4 Factor:Duponchelia fovealis killed by the Bov2 strain of Beauveria bassiana RAW_FILE_NAME=CatM_Bov2_4; RAW_FILE_NAME=-; RAW_FILE_NAME=- SUBJECT_SAMPLE_FACTORS - CatM_Bov2_5 Factor:Duponchelia fovealis killed by the Bov2 strain of Beauveria bassiana RAW_FILE_NAME=CatM_Bov2_5; RAW_FILE_NAME=-; RAW_FILE_NAME=- SUBJECT_SAMPLE_FACTORS - CatM_Bov3_1 Factor:Duponchelia fovealis killed by the Bov3 strain of Beauveria bassiana RAW_FILE_NAME=CatM_Bov3_1; RAW_FILE_NAME=CatM_Bov3_1_R001 CatM_Bov3_1_R002 CatM_Bov3_1_R003 CatM_Bov3_1_R004.; RAW_FILE_NAME=CatM_Bov3_1_R001_NEG CatM_Bov3_1_R002_NEG CatM_Bov3_1_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatM_Bov3_2 Factor:Duponchelia fovealis killed by the Bov3 strain of Beauveria bassiana RAW_FILE_NAME=CatM_Bov3_2; RAW_FILE_NAME=CatM_Bov3_2_R001 CatM_Bov3_2_R002 CatM_Bov3_2_R003 CatM_Bov3_2_R004.; RAW_FILE_NAME=CatM_Bov3_2_R001_NEG CatM_Bov3_2_R002_NEG CatM_Bov3_2_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatM_Bov3_3 Factor:Duponchelia fovealis killed by the Bov3 strain of Beauveria bassiana RAW_FILE_NAME=CatM_Bov3_3; RAW_FILE_NAME=CatM_Bov3_3_R001 CatM_Bov3_3_R002 CatM_Bov3_3_R003 CatM_Bov3_3_R004.; RAW_FILE_NAME=CatM_Bov3_3_R001_NEG CatM_Bov3_3_R002_NEG CatM_Bov3_3_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatM_Bov3_4 Factor:Duponchelia fovealis killed by the Bov3 strain of Beauveria bassiana RAW_FILE_NAME=CatM_Bov3_4; RAW_FILE_NAME=-; RAW_FILE_NAME=- SUBJECT_SAMPLE_FACTORS - CatM_Bov3_5 Factor:Duponchelia fovealis killed by the Bov3 strain of Beauveria bassiana RAW_FILE_NAME=CatM_Bov3_5; RAW_FILE_NAME=-; RAW_FILE_NAME=- SUBJECT_SAMPLE_FACTORS - CatM_Cons_1 Factor:Duponchelia fovealis killed by the consortium RAW_FILE_NAME=CatM_Cons_1; RAW_FILE_NAME=CatM_Cons_1_R001 CatM_Cons_1_R002 CatM_Cons_1_R003 CatM_Cons_1_R004.; RAW_FILE_NAME=CatM_Cons_1_R001_NEG CatM_Cons_1_R002_NEG CatM_Cons_1_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatM_Cons_2 Factor:Duponchelia fovealis killed by the consortium RAW_FILE_NAME=CatM_Cons_2; RAW_FILE_NAME=CatM_Cons_2_R001 CatM_Cons_2_R002 CatM_Cons_2_R003 CatM_Cons_2_R004.; RAW_FILE_NAME=CatM_Cons_2_R001_NEG CatM_Cons_2_R002_NEG CatM_Cons_2_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatM_Cons_3 Factor:Duponchelia fovealis killed by the consortium RAW_FILE_NAME=CatM_Cons_3; RAW_FILE_NAME=CatM_Cons_3_R001 CatM_Cons_3_R002 CatM_Cons_3_R003 CatM_Cons_3_R004.; RAW_FILE_NAME=- SUBJECT_SAMPLE_FACTORS - CatM_Cons_4 Factor:Duponchelia fovealis killed by the consortium RAW_FILE_NAME=CatM_Cons_4; RAW_FILE_NAME=-; RAW_FILE_NAME=- SUBJECT_SAMPLE_FACTORS - CatM_Cons_5 Factor:Duponchelia fovealis killed by the consortium RAW_FILE_NAME=CatM_Cons_5; RAW_FILE_NAME=-; RAW_FILE_NAME=- SUBJECT_SAMPLE_FACTORS - CatS_Bov2_1 Factor:Duponchelia fovealis that survived the application of the Bov 2 strain of Beauveria bassiana RAW_FILE_NAME=CatS_Bov2_1; RAW_FILE_NAME=CatS_Bov2_1_R001 CatS_Bov2_1_R002 CatS_Bov2_1_R003.; RAW_FILE_NAME=CatS_Bov2_1_R001_NEG CatS_Bov2_1_R002_NEG CatS_Bov2_1_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_Bov2_2 Factor:Duponchelia fovealis that survived the application of the Bov 2 strain of Beauveria bassiana RAW_FILE_NAME=CatS_Bov2_2; RAW_FILE_NAME=CatS_Bov2_2_R001 CatS_Bov2_2_R002 CatS_Bov2_2_R003.; RAW_FILE_NAME=CatS_Bov2_2_R001_NEG CatS_Bov2_2_R002_NEG CatS_Bov2_2_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_Bov2_3 Factor:Duponchelia fovealis that survived the application of the Bov 2 strain of Beauveria bassiana RAW_FILE_NAME=CatS_Bov2_3; RAW_FILE_NAME=CatS_Bov2_3_R001 CatS_Bov2_3_R002 CatS_Bov2_3_R003.; RAW_FILE_NAME=CatS_Bov2_3_R001_NEG CatS_Bov2_3_R002_NEG CatS_Bov2_3_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_Bov2_4 Factor:Duponchelia fovealis that survived the application of the Bov 2 strain of Beauveria bassiana RAW_FILE_NAME=CatS_Bov2_4; RAW_FILE_NAME=CatS_Bov2_4_R001 CatS_Bov2_4_R002 CatS_Bov2_4_R003.; RAW_FILE_NAME=CatS_Bov2_4_R001_NEG CatS_Bov2_4_R002_NEG CatS_Bov2_4_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_Bov2_5 Factor:Duponchelia fovealis that survived the application of the Bov 2 strain of Beauveria bassiana RAW_FILE_NAME=CatS_Bov2_5; RAW_FILE_NAME=CatS_Bov2_5_R001 CatS_Bov2_5_R002 CatS_Bov2_5_R003.; RAW_FILE_NAME=CatS_Bov2_5_R001_NEG CatS_Bov2_5_R002_NEG CatS_Bov2_5_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_Bov3_1 Factor:Duponchelia fovealis that survived the application of the Bov 3 strain of Beauveria bassiana RAW_FILE_NAME=CatS_Bov3_1; RAW_FILE_NAME=CatS_Bov3_1_R001 CatS_Bov3_1_R002 CatS_Bov3_1_R003.; RAW_FILE_NAME=CatS_Bov3_1_R001_NEG CatS_Bov3_1_R002_NEG CatS_Bov3_1_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_Bov3_2 Factor:Duponchelia fovealis that survived the application of the Bov 3 strain of Beauveria bassiana RAW_FILE_NAME=CatS_Bov3_2; RAW_FILE_NAME=CatS_Bov3_2_R001 CatS_Bov3_2_R002 CatS_Bov3_2_R003.; RAW_FILE_NAME=CatS_Bov3_2_R001_NEG CatS_Bov3_2_R002_NEG CatS_Bov3_2_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_Bov3_3 Factor:Duponchelia fovealis that survived the application of the Bov 3 strain of Beauveria bassiana RAW_FILE_NAME=CatS_Bov3_3; RAW_FILE_NAME=CatS_Bov3_3_R001 CatS_Bov3_3_R002 CatS_Bov3_3_R003.; RAW_FILE_NAME=CatS_Bov3_3_R001_NEG CatS_Bov3_3_R002_NEG CatS_Bov3_3_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_Bov3_4 Factor:Duponchelia fovealis that survived the application of the Bov 3 strain of Beauveria bassiana RAW_FILE_NAME=CatS_Bov3_4; RAW_FILE_NAME=CatS_Bov3_4_R001 CatS_Bov3_4_R002 CatS_Bov3_4_R003.; RAW_FILE_NAME=CatS_Bov3_4_R001_NEG CatS_Bov3_4_R002_NEG CatS_Bov3_4_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_Bov3_5 Factor:Duponchelia fovealis that survived the application of the Bov 3 strain of Beauveria bassiana RAW_FILE_NAME=CatS_Bov3_5; RAW_FILE_NAME=CatS_Bov3_5_R001 CatS_Bov3_5_R002 CatS_Bov3_5_R003.; RAW_FILE_NAME=CatS_Bov3_5_R001_NEG CatS_Bov3_5_R002_NEG CatS_Bov3_5_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_Cons_1 Factor:Duponchelia fovealis that survived the application of the consortium RAW_FILE_NAME=CatS_Cons_1; RAW_FILE_NAME=CatS_Cons_1_R001 CatS_Cons_1_R002 CatS_Cons_1_R003.; RAW_FILE_NAME=CatS_Cons_1_R001_NEG CatS_Cons_1_R002_NEG CatS_Cons_1_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_Cons_2 Factor:Duponchelia fovealis that survived the application of the consortium RAW_FILE_NAME=CatS_Cons_2; RAW_FILE_NAME=CatS_Cons_2_R001 CatS_Cons_2_R002 CatS_Cons_2_R003.; RAW_FILE_NAME=CatS_Cons_2_R001_NEG CatS_Cons_2_R002_NEG CatS_Cons_2_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_Cons_3 Factor:Duponchelia fovealis that survived the application of the consortium RAW_FILE_NAME=CatS_Cons_3; RAW_FILE_NAME=CatS_Cons_3_R001 CatS_Cons_3_R002 CatS_Cons_3_R003.; RAW_FILE_NAME=CatS_Cons_3_R001_NEG CatS_Cons_3_R002_NEG CatS_Cons_3_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_Cons_4 Factor:Duponchelia fovealis that survived the application of the consortium RAW_FILE_NAME=-; RAW_FILE_NAME=CatS_Cons_4_R001 CatS_Cons_4_R002 CatS_Cons_4_R003.; RAW_FILE_NAME=CatS_Cons_4_R001_NEG CatS_Cons_4_R002_NEG CatS_Cons_4_R003_NEG. SUBJECT_SAMPLE_FACTORS - CatS_Cons_5 Factor:Duponchelia fovealis that survived the application of the consortium RAW_FILE_NAME=-; RAW_FILE_NAME=CatS_Cons_5_R001 CatS_Cons_5_R002 CatS_Cons_5_R003.; RAW_FILE_NAME=CatS_Cons_5_R001_NEG CatS_Cons_5_R002_NEG CatS_Cons_5_R003_NEG. #COLLECTION CO:COLLECTION_SUMMARY Groups of 10 third instar caterpillars were placed on strawberry leaves and CO:COLLECTION_SUMMARY sprayed with 1 mL of B. bassiana conidia-suspension: 2 x 107 conidia/mL in 0.85% CO:COLLECTION_SUMMARY saline solution with Tween 80® adhesive spreader added. Therefore, the CO:COLLECTION_SUMMARY treatments applied were: control caterpillars (consisting only of 0.85% saline CO:COLLECTION_SUMMARY solution and Tween 80®), caterpillars inoculated with Bov 3 strain of B. CO:COLLECTION_SUMMARY bassiana, caterpillars inoculated with Bov 2 strain of B. bassiana and CO:COLLECTION_SUMMARY caterpillars inoculated with the Bov3-Bov 2 consortium. On the seventh day, CO:COLLECTION_SUMMARY caterpillars that did not respond to the stimulus provided by the touch of a CO:COLLECTION_SUMMARY brush were considered dead. All caterpillars were identified as living or dead CO:COLLECTION_SUMMARY at the end of the seven days and were subsequently stored in a freezer at CO:COLLECTION_SUMMARY -80ºC. CO:COLLECTION_PROTOCOL_FILENAME Metabolite_Extraction CO:SAMPLE_TYPE Insect tissue CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY The treatments applied were: control caterpillars (consisting only of 0.85% TR:TREATMENT_SUMMARY saline solution and Tween 80®), caterpillars inoculated with Bov 3 strain of B. TR:TREATMENT_SUMMARY bassiana, caterpillars inoculated with Bov 2 strain of B. bassiana and TR:TREATMENT_SUMMARY caterpillars inoculated with the Bov3-Bov2 consortium. TR:TREATMENT_PROTOCOL_FILENAME Metabolite_Extraction TR:TREATMENT_DOSEVOLUME 2 x 10^7 conidia/mL #SAMPLEPREP SP:SAMPLEPREP_SUMMARY After the direct contact bioassay, the caterpillars were arranged by treatment, SP:SAMPLEPREP_SUMMARY then macerated in liquid nitrogen (N2). Extraction was performed with 200 mg of SP:SAMPLEPREP_SUMMARY the macerate added to a 1 mL microtube (Eppendorf, Germany) previously treated SP:SAMPLEPREP_SUMMARY with methanol. Following this, 125 μL of chloroform (CHCl3), 50 μL ultra-pure SP:SAMPLEPREP_SUMMARY water (H2O), and 250 μL cold methanol (CH3OH) were added to the macerate. The SP:SAMPLEPREP_SUMMARY microtubes were vigorously vortexed and placed in an ultrasonic bath SP:SAMPLEPREP_SUMMARY (Odontobrás, Ribeirão-SP) at 20 Hz and approximately 4ºC for 10 minutes. SP:SAMPLEPREP_SUMMARY Then, 50 μL of CHCl3 and 50 μL of H2O were added, and the tubes were vortexed SP:SAMPLEPREP_SUMMARY again. The samples were centrifuged (Eppendorf, Germany) for 5 minutes at 14000 SP:SAMPLEPREP_SUMMARY rpm and 4°C, and the supernatant was filtered on a Whatman® 0.22 µm filter SP:SAMPLEPREP_SUMMARY (Merck, Germany) and transferred to a glass vial. The vial was taken to a SP:SAMPLEPREP_SUMMARY lyophilizer (Thermo Fischer Scientific, MA) until the samples had completely SP:SAMPLEPREP_SUMMARY dried. Finally, the lyophilized samples were resuspended in 200 µL of SP:SAMPLEPREP_SUMMARY extraction solution and aliquoted for use in the GC-MS and LC-MS/MS. SP:SAMPLEPREP_PROTOCOL_FILENAME Metabolite_Extraction SP:EXTRACT_STORAGE -80℃ #CHROMATOGRAPHY CH:METHODS_FILENAME Metabolomics_Methods CH:INSTRUMENT_NAME Agilent 7890A CH:COLUMN_NAME Agilent DB-5 (20m x 0.18mm, 0.18um); Restek RX-T 17 (0.9m x 0.10mm, 0.10um) CH:COLUMN_TEMPERATURE 70 - 320 ºC CH:FLOW_RATE 1 mL.min-1 CH:INJECTION_TEMPERATURE 280 ºC CH:SAMPLE_INJECTION 1 uL CH:OVEN_TEMPERATURE 70°C for 2 min, increasing by 15°C·min-1 until it reached 320°C and then CH:OVEN_TEMPERATURE held at this temperature for 4 min. CH:CHROMATOGRAPHY_TYPE GC #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE metabolomics_methods.pdf #MS MS:INSTRUMENT_NAME Leco Pegasus 4D GCxGC TOF MS:INSTRUMENT_TYPE GC x GC-TOF MS:MS_TYPE API MS:MS_COMMENTS Data from GC-MS was processed using ChromaTOF 4.32 software to conduct baseline MS:MS_COMMENTS correction, deconvolution, retention index (RI), retention time correction (RT), MS:MS_COMMENTS identification, and alignment of peaks. NIST library version 11 was used for the MS:MS_COMMENTS identification of metabolites. Only metabolites with a score of 700 or above MS:MS_COMMENTS were considered. The intensity of each metabolite was normalized by the total MS:MS_COMMENTS ion count (TIC) of each sample. Statistical analyses were performed using the MS:MS_COMMENTS MetaboAnalyst 4.0 online software (available at MS:MS_COMMENTS http://www.metaboanalyst.ca/MetaboAnalyst/) MS:ION_MODE UNSPECIFIED MS:FRAGMENTATION_METHOD EI MS:ION_SOURCE_TEMPERATURE 250 ºC MS:IONIZATION_ENERGY 70 eV #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS peak area MS_METABOLITE_DATA_START Samples CatM_Bov2_1 CatM_Bov2_2 CatM_Bov2_3 CatM_Bov2_4 CatM_Bov2_5 CatM_Bov3_1 CatM_Bov3_2 CatM_Bov3_3 CatM_Bov3_4 CatM_Bov3_5 CatM_Cons_1 CatM_Cons_2 CatM_Cons_3 CatM_Cons_4 CatM_Cons_5 Factors Factor:Duponchelia fovealis killed by the Bov2 strain of Beauveria bassiana Factor:Duponchelia fovealis killed by the Bov2 strain of Beauveria bassiana Factor:Duponchelia fovealis killed by the Bov2 strain of Beauveria bassiana Factor:Duponchelia fovealis killed by the Bov2 strain of Beauveria bassiana Factor:Duponchelia fovealis killed by the Bov2 strain of Beauveria bassiana Factor:Duponchelia fovealis killed by the Bov3 strain of Beauveria bassiana Factor:Duponchelia fovealis killed by the Bov3 strain of Beauveria bassiana Factor:Duponchelia fovealis killed by the Bov3 strain of Beauveria bassiana Factor:Duponchelia fovealis killed by the Bov3 strain of Beauveria bassiana Factor:Duponchelia fovealis killed by the Bov3 strain of Beauveria bassiana Factor:Duponchelia fovealis killed by the consortium Factor:Duponchelia fovealis killed by the consortium Factor:Duponchelia fovealis killed by the consortium Factor:Duponchelia fovealis killed by the consortium Factor:Duponchelia fovealis killed by the consortium 1_2-Ethenediol 11.6437 1.7773 1.6177 1.6505 1_3-Butanediol 0.4854 1.6591 0.7258 0.2979 0.3366 2.4587 1.4462 0.5842 1.7123 2.8143 0.6915 0.8408 0.2913 1.3573 1.1594 1-ethyl-1-(2-methylpropyl)-Hydrazine 56.1820 0.7589 0.5769 1-Isobutylsulfanylmethyl-2_8_9-trioxa-5-aza-1-sila-bicyclo[3.3.3]undecane 0.0132 0.0143 3.3526 0.0625 1-Monopalmitin 3.0939 1.6014 1.5074 1.7853 1.8057 3.4314 4.9917 4.7009 1.7661 1.8658 5.9202 6.9421 6.1541 0.8747 0.6667 2_5-Dimethoxymandelic acid 1.2517 1.4976 1.9375 1.4998 2-deoxy-D-Erythro-Pentopyranose 2.1095 2.8376 2.4214 (2-Ethoxyethoxy)acetic acid 1.8849 20.7517 40.0114 2-Hydroxyglutaric acid 0.0042 0.2251 0.0131 0.3891 0.4681 0.1162 0.1206 0.2934 0.1267 0.0436 2-Keto-d-gluconic acid 5.1555 1.2055 6.3793 6.3587 16.2124 22.5834 3.6111 9.4951 9.9160 5.2547 5.9100 6.3517 6.6682 0.9434 2-Palmitoylglycerol 0.2177 0.1005 0.1231 0.1279 0.0274 0.2005 0.1905 2-pyrrolidinone 0.0583 0.1255 0.0990 0.2154 0.1923 0.0960 0.1241 0.6099 0.0737 0.1189 0.1286 0.0324 0.0324 4-Aminobutanoic acid 2.4381 4.5888 3.2182 36.9231 37.0840 7.1998 8.8670 43.1514 6.7780 22.5124 7.1886 5.5089 11.8547 7.4206 5.1672 5-Aminovaleric acid 3.7840 2.7216 2.4834 2.7352 0.6454 0.3226 0.9497 2.0039 5-Methyluridine 0.4578 0.2482 0.2856 Acetic acid 0.0971 0.1188 0.0956 0.0872 0.1027 0.2123 0.2965 0.2823 0.2690 0.3003 0.0576 0.1912 0.1991 0.1500 0.1277 Adenine 0.4853 0.3994 0.2944 0.5648 0.5788 0.6675 1.0137 0.8824 0.7872 0.6152 0.4245 0.5853 0.2611 0.3753 0.2105 Adrenaline 0.0435 0.0242 0.0322 0.2265 0.0271 0.4616 0.3569 0.7508 0.0072 0.0038 1.0209 Ala-beta-Ala 0.1210 0.3114 0.6884 0.7340 0.3671 1.0606 0.2479 2.9563 3.7774 3.2569 0.1583 Alanine 43.9279 16.4776 57.4986 46.3101 12.9065 28.4375 49.9740 35.0902 38.5073 25.3995 54.4907 35.4120 22.4003 136.2271 56.7810 Alanylglycine 0.6524 0.4553 0.4056 3.0860 0.4520 0.6853 0.8318 0.6559 0.2862 alpha-D-Mannopyranose 7.4181 6.8052 4.0228 alpha-D-(+)-Talopyranose 1.6021 2.1436 1.2277 3.1059 1.8530 0.6455 0.8617 0.5127 0.6776 0.1447 0.2065 1.7790 alpha-Methyl-L-tyrosine 0.1259 0.1287 0.0879 Aminomalonic acid 0.4906 0.2667 0.1347 0.5163 0.4785 3.3088 2.1076 0.9734 1.4117 2.3574 0.3035 0.4361 0.1096 0.8519 0.5088 Ammelide 3.1327 6.4077 1.7936 67.6676 111.3117 1.7407 0.0723 5.0717 0.0028 3.9970 2.1334 2.9434 54.7204 Asparagine 1.5849 0.7786 0.8730 0.5862 0.2546 2.8683 1.6284 0.4504 0.1855 2.6042 3.1136 1.4152 1.1536 Atenolol 0.2688 1.1726 0.4058 0.2690 0.9681 0.9035 0.3142 0.5721 Aucubin 0.2651 0.1480 0.1677 0.1812 0.2149 2.3429 3.3708 3.3323 0.4421 0.5665 0.0731 0.1503 0.1036 0.2955 0.1036 b-Aminoisobutyric acid 0.1452 0.2665 0.0301 Bis[2-(trimethylsilyloxy)ethyl] Ether 47.9284 0.1497 28.9524 0.1367 0.3532 0.2095 Butanedioic acid 18.4730 9.7132 2.9787 11.9970 10.0121 16.7088 1.1112 16.5939 20.2620 13.5814 21.5386 16.5831 Cadaverine 5.4603 0.5085 0.5710 0.6026 1.1376 5.2660 1.2617 0.5206 1.2207 1.6608 1.6917 1.9392 0.8325 0.4626 Citric acid 26.4398 27.1508 24.8448 29.0227 22.4088 10.6546 13.2212 43.2110 77.6908 48.2795 27.6014 29.3803 44.6368 95.0874 35.4454 Citrulline 0.5496 24.0226 0.8536 0.8177 0.4531 11.1846 0.8580 2.1533 28.3020 0.8210 46.8782 Cystathionine 0.4650 0.4229 0.1291 0.4148 0.5222 0.5045 0.3840 0.3301 Cysteine 0.5390 0.5777 0.3537 0.2080 0.9834 1.1305 0.7059 0.2267 0.1121 Cytidine 0.1154 0.1812 0.1517 Deoxyglucose 0.3739 0.3346 0.2720 0.2617 0.2413 0.1927 0.3101 0.1347 Deoxyguanosine 0.1807 0.2044 0.2897 D-Galactofuranose phosphate 0.5683 0.1697 0.1637 D-Galactose 9.4776 7.5017 5.7149 28.6442 30.3804 9.2193 0.9426 D-(+)-Galacturonic acid 15.4553 4.6882 3.7964 5.3091 5.7167 0.3445 0.5516 11.3347 1.6527 2.2071 2.9864 2.6022 1.9740 D-Glucitol 86.8049 69.5923 60.7289 59.2372 25.2667 31.9421 23.4032 Diisooctyl phthalate 0.1813 0.1249 0.1849 0.2122 0.2365 0.3730 0.1721 0.2390 0.5632 0.4306 0.3980 0.4431 0.3470 0.2507 D-(-)-Lyxofuranose 0.8014 1.6406 1.5339 0.1531 D-Mannose 3.2042 7.0445 4.2503 D-Psicofuranos 0.8720 1.4920 27.1885 D-Sorbitol 18.6771 13.6106 1.9270 75.8462 75.2367 D-(-)-Tagatofuranose 7.0012 4.9436 23.6760 D-Xylopyranose 0.1875 0.2808 0.3612 0.9313 0.2831 0.9782 0.1558 0.4338 D-Xylose 0.1098 0.0732 0.6192 0.2212 Erythronic acid 0.0263 0.0236 0.0517 0.3942 0.4462 0.1636 Ethyl .alpha.-D-glucopyranoside 0.3823 0.2724 0.5915 0.6527 1.0105 1.7123 1.6323 0.2811 0.2752 0.6712 0.3559 0.4377 0.5086 0.5371 Ethyl phosphoric acid 0.0118 0.0204 0.0052 Formamide 0.7202 1.6121 1.9727 1.0812 4.6452 5.4353 2.8630 1.7747 5.5176 5.9845 3.5628 2.5089 1.5805 Fumaric acid 3.0067 4.4478 4.3142 4.4688 1.5928 3.7457 4.3741 6.3460 6.7811 5.8486 5.0862 3.7785 Galactaric acid 3.1797 1.7677 1.4756 1.9544 1.9122 1.0222 0.9035 0.2255 0.3001 0.9976 1.4486 1.7023 1.5623 1.1344 Gallic acid 6.5938 3.9105 3.8224 Glucopyranose 4.8799 6.4081 3.1695 6.2832 3.2365 5.1509 6.7603 12.0447 4.1318 8.1700 2.8024 2.4047 6.7966 4.4998 3.1165 Glucopyranose phosphate 0.1455 0.1122 0.1528 Glucosamine 0.1058 0.0135 6.3938 Glyceric acid 1.7693 1.3018 1.2692 1.3772 2.0287 1.8849 3.2282 1.8683 1.8479 4.6497 1.6239 1.9711 2.7423 1.1679 0.9815 Glycerol 94.1488 64.4952 102.2326 31.5708 42.3293 31.0047 94.7552 17.4308 138.9142 127.1054 94.7948 92.9345 33.2215 186.4924 72.4514 Glycerol-3-phosphate 11.7060 36.3426 7.7119 33.0975 4.1308 12.0509 8.3832 35.6259 2.1738 2.5983 2.0775 101.4519 99.2708 Glycerol monostearate 1.2747 1.0418 1.0739 0.9892 0.9691 1.2770 1.8235 1.6050 1.7928 1.7836 0.8707 0.6064 0.6088 1.4464 1.2209 Glyceryl-glycoside 1.1910 0.4588 0.0192 0.0269 0.2245 0.0110 0.0100 0.0261 0.2500 0.4811 Glycine 6.6495 22.7722 12.2476 12.8227 12.5981 39.7178 40.3761 34.2088 79.9298 40.7321 17.9057 21.7735 16.0432 23.1839 17.6147 Guanine 1.5584 1.3031 1.6069 1.6441 2.1095 2.8376 0.3834 0.8588 Guanosine 1.9409 1.4144 0.7827 1.5309 1.5556 2.1643 3.0930 3.0451 1.6270 1.8441 1.5001 1.8405 1.7524 0.7970 0.4597 Gulonic acid 35.8365 27.0151 24.7220 25.1380 24.6862 66.6600 43.4758 42.6660 34.0883 37.6579 32.7189 21.1632 26.4415 17.2539 12.7161 Hypoxanthine 2.7184 106.1113 23.9182 22.5723 21.4072 Inosine 4.5127 3.6165 3.3920 4.0324 4.0801 4.5542 6.4436 6.1743 4.8395 5.5132 5.1423 6.1593 5.8031 3.6442 2.6280 Isoleucine methyl ester 0.0381 0.0140 0.0443 Isoxanthopterin 0.0267 0.1109 0.0707 0.1082 0.1233 0.1341 0.1444 0.1802 0.1064 0.1773 0.1944 0.1348 0.0665 Lactic acid 1.1773 0.5536 0.2968 0.5641 0.5189 0.6402 0.8946 0.7297 2.5791 2.6426 1.3303 0.2396 0.5922 0.5151 0.5079 L-Aspartic acid 16.9938 24.2749 18.8212 15.4097 25.7288 13.8540 27.2402 13.4880 39.4451 50.4963 17.4644 20.0021 20.5647 14.2629 19.7609 Leu-Gly 2.7432 2.1577 1.8798 3.8780 0.6470 2.4159 0.4441 3.5221 1.3108 1.2263 0.4193 3.3658 2.6257 1.9266 0.1244 Levodopa 6.4073 4.3136 4.7364 3.7645 4.1443 4.8032 L-fucose 0.1168 0.1520 1.3029 0.4086 0.2695 0.3350 0.5270 0.1232 0.6883 0.5425 0.9601 0.7876 0.4529 0.2880 0.2346 L-Glutamic acid 5.5030 5.0309 2.7676 2.4667 2.2065 6.8477 6.0328 3.8373 4.3747 5.9733 4.2665 5.0834 2.6630 4.2553 3.7395 l-Glutamine 60.1921 7.8568 10.7296 7.1062 58.4424 23.1127 L-Histidine 4.5433 6.3908 5.2620 6.0289 6.0274 19.7116 13.8610 13.6425 9.3385 10.3015 7.1005 10.7985 6.5891 10.1763 6.3938 L-Homocystine_ [S-(R*_R*)]- 0.3379 0.4365 0.3340 0.3274 0.4029 L-Homoserine 0.0643 0.0842 0.0082 L-Isoleucine 2.7529 10.8446 4.6445 10.1328 0.9304 4.8740 7.0565 10.2900 9.8176 21.5615 4.1402 3.6433 7.8087 7.1866 5.5573 L-Leucine 1.9344 0.3650 0.8426 23.8697 4.2505 1.3798 2.6036 6.5468 2.3731 3.5473 0.6327 92.9345 48.0891 0.6858 0.7792 L-Lysine 22.6210 22.7746 21.3742 19.7445 18.5342 25.9385 34.7660 28.7864 29.1144 23.0200 19.2569 27.3799 24.5598 19.4520 15.1451 L-Methionine 24.5661 0.0433 13.0266 11.8986 0.0871 0.1835 0.5057 0.2853 17.6622 20.7903 14.1020 L-Norleucine 2.4849 0.2983 0.1067 0.1229 0.0675 L-Proline 23.8933 34.0164 24.8061 15.7015 30.6810 16.8173 19.6091 19.1459 26.5760 26.2541 27.9823 31.0997 29.0294 27.7539 19.8657 L-(+)-Tartaric acid 0.1890 0.0482 0.1684 0.0983 0.0867 L-threonine 13.7960 16.5185 13.8994 20.5529 17.5620 9.7234 19.5396 10.9537 19.7452 31.2587 14.7358 15.9697 14.1249 15.1808 10.4369 L-Tryptophan 2.2582 2.0758 1.7160 2.1290 2.1501 2.4294 3.4214 2.6728 2.0345 1.6117 2.1409 1.8874 1.3264 1.6966 1.2478 L-Valyl-L-leucine 1.2393 0.3433 0.2502 0.4790 1.2950 1.5646 0.3659 3.0425 0.3001 1.1080 2.1786 3.6969 0.2939 0.1179 Lyxose 23.8526 1.3953 0.3835 0.0268 0.6269 0.1523 1.2107 1.0484 0.3126 Malic acid 3.2312 1.8535 1.8918 2.1879 2.1807 4.3710 5.9016 6.0321 4.4866 5.4263 2.8710 3.4030 2.8094 2.5057 1.5031 Mannitol 1.2798 62.2103 16.5020 58.2490 17.3566 42.0636 67.9882 0.8040 59.9713 meso-Erythritol 94.4983 27.4795 24.7751 28.0883 28.5566 39.4467 49.8778 42.2427 21.1278 35.0107 24.2364 27.5434 33.7165 16.5135 10.8540 Methyl galactoside 1.2555 0.6700 0.1110 0.2257 0.2672 0.3544 Methyl phosphate 2.3767 2.6106 2.6915 2.9821 3.3988 18.6210 27.6730 24.7560 12.6648 14.1090 0.5260 1.1700 1.8478 2.5865 2.5214 Mevalonic acid 0.5777 0.3537 0.3608 0.3661 0.6396 1.2905 0.6890 0.3586 0.2943 Monostearin 1.6733 0.5763 0.4009 0.6746 0.6772 1.2614 1.8368 1.7461 0.6030 0.7004 2.4298 2.5825 2.5226 0.3172 0.3701 Myo-Inositol 0.8296 0.6465 0.9211 0.9297 1.6927 2.4297 2.2314 1.6859 1.3367 0.6468 0.7754 1.0825 0.5636 Myoinositol 1-phosphate 10.3450 6.4024 5.6889 6.6176 6.6926 10.0963 14.1470 13.4579 11.2180 12.8566 4.9264 4.9188 5.6091 5.8860 5.4866 N-Acetyl-D-glucosamine 9.8823 5.3946 3.9582 4.7840 6.0146 5.7273 8.1367 1.2224 17.7991 8.5921 8.9214 5.6560 5.8860 5.5972 N-alpha-Acetyl-L-Lysine 0.4624 0.3733 0.7925 0.5475 0.6934 0.5513 0.7262 0.9820 0.2673 2.0672 N-ethoxycarbonyl-_ nonyl ester_ L-Norvaline 0.0010 0.0776 0.0402 N-methoxycarbonyl-_ isohexyl ester_ L-Norleucine 0.0031 0.0024 0.0049 0.0039 0.0613 0.9517 10.7985 6.2976 0.1358 N-Methyl-L-prolinol 0.1095 0.0628 0.0254 0.5428 N-methyl-n-butoxycarbonyl-_ octyl ester_ Alanine 0.0978 0.0831 0.1080 0.1481 0.0471 0.0027 0.0000 0.0547 N-methyl-N-methoxycarbonyl-_ undecyl ester_ Alanine 6.9182 1.2001 1.4743 53.0408 11.2482 25.2008 32.7045 45.0771 16.9148 Norephedrine 1.0947 0.1123 0.1104 Ornithine 2.4826 1.4042 1.9596 1.3239 2.1624 4.6032 7.1592 7.3952 3.9820 2.1008 Oxalic acid 0.0690 5.4762 0.5619 0.5852 0.4137 0.8077 0.9229 0.2785 0.3006 0.0357 0.4482 1.3217 0.6928 1.3220 1.1617 Palmitic Acid 1.7222 1.4299 1.7029 1.1356 1.9320 2.4250 2.1975 2.4183 3.3524 1.0724 1.0712 1.3624 3.2653 2.0745 Phenylalanine 10.3414 11.5457 10.4565 13.3785 11.2624 9.5458 10.2923 21.5804 12.0345 7.2756 10.5899 11.7654 19.6881 8.4079 6.4353 Phenylpropanolamine 0.2117 30.4112 122.4619 161.6409 71.2769 20.5975 29.0989 0.8665 214.7949 68.5791 106.2450 38.1594 192.6945 Pipecolic acid 0.2024 0.0661 0.3568 0.3005 2.8060 39.4581 50.2497 40.9455 0.0254 5.1731 42.3784 0.7152 20.3597 0.1617 28.2828 Propanedioic acid 0.3467 0.2230 0.0893 Pseudo uridine 0.1615 0.1969 0.1513 0.1611 0.1648 0.2707 0.3702 0.3579 0.1325 0.2070 0.1893 0.1847 0.1101 0.1406 0.1288 Putrescine 0.3924 1.2038 1.0109 1.2486 0.5155 1.2084 1.5438 0.7093 0.0277 0.1143 2.0696 2.9109 2.0436 1.5930 0.8723 Pyroglutamic acid 21.4138 48.3673 76.5664 86.3742 74.9604 96.7748 32.7356 50.6238 133.4866 66.0394 49.8177 56.3160 51.2270 67.2944 Ribitol 158.5848 54.5074 34.0552 31.6686 102.0316 33.1361 42.3826 151.0420 10.4083 90.9269 68.3602 36.6941 10.9201 4.8084 65.1463 Ribonic acid 1.3489 1.3424 1.3314 0.9557 0.3569 1.2522 Serine 12.0005 18.1775 16.2698 12.0891 9.0576 6.9699 15.3084 8.3361 10.2648 18.2233 17.7956 19.1235 16.0158 17.5862 12.0877 Thymine 0.0993 0.0925 0.0688 0.1085 0.1531 0.0381 0.0565 0.1175 0.0599 0.1239 0.2020 0.0798 0.1937 0.0656 trans-9-Octadecenoic acid 0.9223 0.2880 1.3572 0.4992 0.5737 0.7545 1.1293 0.8295 Tyramine 1.5099 0.2485 0.2519 0.3276 0.3229 0.3598 0.7729 0.8261 0.4555 0.2708 1.0467 0.5427 1.2817 0.5555 0.2113 Tyrosine 4.0735 2.6237 0.8384 1.6537 1.9949 0.7489 1.6818 1.0447 0.0154 0.0845 3.8607 5.5194 4.9885 6.3483 1.8724 Uracil 6.4752 4.4478 4.4134 4.6106 1.7381 4.7966 3.8786 3.7457 5.3681 6.0464 6.7811 5.8437 5.0862 3.7785 Urea 99.7790 100.4465 54.4340 22.8045 73.2931 184.2774 46.6030 63.0368 41.3864 21.2280 64.0497 99.4458 72.3316 40.0331 29.2954 Uridine 2.5177 2.2165 2.4709 2.8452 2.5891 3.1329 4.8726 3.7059 2.1347 2.2099 2.5258 3.2995 2.8429 1.5998 1.0238 Valine 3.5319 10.1639 4.9150 9.6703 9.1973 13.3294 19.3930 18.4157 12.6084 12.1037 22.9595 13.2054 11.7258 8.4321 6.3762 Val-Val 0.9976 0.3842 1.7023 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant 1,2-Ethenediol 1,3-Butanediol 1-ethyl-1-(2-methylpropyl)-Hydrazine 1-Isobutylsulfanylmethyl-2,8,9-trioxa-5-aza-1-sila-bicyclo[3.3.3]undecane 1-Monopalmitin 2,5-Dimethoxymandelic acid 2-deoxy-D-Erythro-Pentopyranose (2-Ethoxyethoxy)acetic acid 2-Hydroxyglutaric acid 2-Keto-d-gluconic acid 2-Palmitoylglycerol 2-pyrrolidinone 4-Aminobutanoic acid 5-Aminovaleric acid 5-Methyluridine Acetic acid Adenine Adrenaline Ala-beta-Ala Alanine Alanylglycine alpha-D-Mannopyranose alpha-D-(+)-Talopyranose alpha-Methyl-L-tyrosine Aminomalonic acid Ammelide Asparagine Atenolol Aucubin b-Aminoisobutyric acid Bis[2-(trimethylsilyloxy)ethyl] Ether Butanedioic acid Cadaverine Citric acid Citrulline Cystathionine Cysteine Cytidine Deoxyglucose Deoxyguanosine D-Galactofuranose phosphate D-Galactose D-(+)-Galacturonic acid D-Glucitol Diisooctyl phthalate D-(-)-Lyxofuranose D-Mannose D-Psicofuranos D-Sorbitol D-(-)-Tagatofuranose D-Xylopyranose D-Xylose Erythronic acid Ethyl .alpha.-D-glucopyranoside Ethyl phosphoric acid Formamide Fumaric acid Galactaric acid Gallic acid Glucopyranose Glucopyranose phosphate Glucosamine Glyceric acid Glycerol Glycerol-3-phosphate Glycerol monostearate Glyceryl-glycoside Glycine Guanine Guanosine Gulonic acid Hypoxanthine Inosine Isoleucine methyl ester Isoxanthopterin Lactic acid L-Aspartic acid Leu-Gly Levodopa L-fucose L-Glutamic acid l-Glutamine L-Histidine L-Homocystine, [S-(R*,R*)]- L-Homoserine L-Isoleucine L-Leucine L-Lysine L-Methionine L-Norleucine L-Proline L-(+)-Tartaric acid L-threonine L-Tryptophan L-Valyl-L-leucine Lyxose Malic acid Mannitol meso-Erythritol Methyl galactoside Methyl phosphate Mevalonic acid Monostearin Myo-Inositol Myoinositol 1-phosphate N-Acetyl-D-glucosamine N-alpha-Acetyl-L-Lysine N-ethoxycarbonyl-, nonyl ester, L-Norvaline N-methoxycarbonyl-, isohexyl ester, L-Norleucine N-Methyl-L-prolinol N-methyl-n-butoxycarbonyl-, octyl ester, Alanine N-methyl-N-methoxycarbonyl-, undecyl ester, Alanine Norephedrine Ornithine Oxalic acid Palmitic Acid Phenylalanine Phenylpropanolamine Pipecolic acid Propanedioic acid Pseudo uridine Putrescine Pyroglutamic acid Ribitol Ribonic acid Serine Thymine trans-9-Octadecenoic acid Tyramine Tyrosine Uracil Urea Uridine Valine Val-Val METABOLITES_END #END