#METABOLOMICS WORKBENCH yli_20211223_093409 DATATRACK_ID:3006 STUDY_ID:ST002050 ANALYSIS_ID:AN003337 VERSION 1 CREATED_ON 09-06-2023 #PROJECT PR:PROJECT_TITLE Perfluoroalkyl Compounds and Child Metabolic Health (Healthy Start Cohort) PR:PROJECT_TYPE Metabolomics PR:PROJECT_SUMMARY The project Perfluoroalkyl Compounds and Child Metabolic Health is funded by the PR:PROJECT_SUMMARY NIH Environmental influences on Child Health Outcomes (ECHO) program, and uses PR:PROJECT_SUMMARY data from the Healthy Start and Project Viva ECHO cohorts. This project will PR:PROJECT_SUMMARY examine associations of untargeted metabolomics data acquired from cord blood PR:PROJECT_SUMMARY with prenatal PFAS exposure and the effect on infant/child metabolic health. PR:INSTITUTE NC HHEAR Hub PR:DEPARTMENT Untargeted Analysis PR:LABORATORY Sumner/Li Lab PR:LAST_NAME Sumner, Li PR:FIRST_NAME Susan, Yuan PR:ADDRESS 500 Laureate Way, Kannapolis, NC 28081 PR:EMAIL susan_sumner@unc.edu, yuanyli4@unc.edu PR:PHONE 9196224456 PR:FUNDING_SOURCE This research was supported by the Environmental influences on Child Health PR:FUNDING_SOURCE Outcomes (ECHO) program, Office of The Director, National Institutes of Health. PR:FUNDING_SOURCE The content is solely the responsibility of the authors and does not necessarily PR:FUNDING_SOURCE represent the official views of the National Institutes of Health. Healthy Start PR:FUNDING_SOURCE is an ECHO cohort which is supported by the following ECHO Program PR:FUNDING_SOURCE Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, PR:FUNDING_SOURCE Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data PR:FUNDING_SOURCE Analysis Center: Johns Hopkins University Bloomberg School of Public Health, PR:FUNDING_SOURCE Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North PR:FUNDING_SOURCE Carolina: Parker CB; U24 OD023382 North Carolina Human Health Exposure Analysis PR:FUNDING_SOURCE Resource Hub: Research Triangle Institute: Fennell T, University of North PR:FUNDING_SOURCE Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du PR:FUNDING_SOURCE X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: PR:FUNDING_SOURCE Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539 PR:DOI http://dx.doi.org/10.21228/M86697 #STUDY ST:STUDY_TITLE Perfluoroalkyl Compounds and Child Metabolic Health (Project Viva) ST:STUDY_SUMMARY Project Viva: Pregnant women were enrolled in Project Viva between 1999 and 2002 ST:STUDY_SUMMARY at their first prenatal visit at one of 8 obstetric clinics of Atrius Harvard ST:STUDY_SUMMARY Vanguard Medical Associates, a multispecialty group practice in eastern ST:STUDY_SUMMARY Massachusetts. Eligible mothers were fluent in English, had singleton ST:STUDY_SUMMARY gestations, were <22 weeks gestation, and had no plans to move away from the ST:STUDY_SUMMARY study area. Research staff performed in-person study visits with participating ST:STUDY_SUMMARY mothers in the first (median gestational age 9.9 weeks) and second (median ST:STUDY_SUMMARY gestational age 28.1 weeks) trimesters of pregnancy, and with mothers and ST:STUDY_SUMMARY children during the first few days after delivery, during infancy (median age ST:STUDY_SUMMARY 6.3 months), in early childhood (median age 3.3 years), mid-childhood (median ST:STUDY_SUMMARY age 7.7 years), and adolescence (median age13 years). In this analysis, we will ST:STUDY_SUMMARY use data from 188 mother-child pairs in Project Viva with available information ST:STUDY_SUMMARY on prenatal PFAS concentrations, available umbilical cord serum samples at ST:STUDY_SUMMARY delivery, and outcomes of interest. Please contact Nicole Bornkamp at ST:STUDY_SUMMARY Nicole_Bornkamp@harvardpilgrim.org for questions related to the subject ST:STUDY_SUMMARY characteristics and outcomes. This research was supported by the Environmental ST:STUDY_SUMMARY influences on Child Health Outcomes (ECHO) program, Office of The Director, ST:STUDY_SUMMARY National Institutes of Health. The content is solely the responsibility of the ST:STUDY_SUMMARY authors and does not necessarily represent the official views of the National ST:STUDY_SUMMARY Institutes of Health. Project Viva is an ECHO cohort which is supported by the ST:STUDY_SUMMARY following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical ST:STUDY_SUMMARY Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C ST:STUDY_SUMMARY OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of ST:STUDY_SUMMARY Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, ST:STUDY_SUMMARY Durham, North Carolina: Parker CB; U24 OD023382 North Carolina Human Health ST:STUDY_SUMMARY Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, ST:STUDY_SUMMARY University of North Carolina at Chapel Hill: Sumner S, University of North ST:STUDY_SUMMARY Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis ST:STUDY_SUMMARY Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; ST:STUDY_SUMMARY U24 ES026539 ST:INSTITUTE Harvard Pilgrim Health Care Institute ST:DEPARTMENT Population Medicine ST:LABORATORY Channing ST:LAST_NAME Oken ST:FIRST_NAME Emily ST:ADDRESS 401 Park Drive Ste 401, Boston, MA 02215 ST:EMAIL emily_oken@harvardpilgrim.org ST:PHONE 617-867-4835 ST:SUBMIT_DATE 2021-12-23 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:GENDER Male and female #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS N/A HHEAR_10_Oken Study type:HHEAR reference material RAW_FILE_NAME=HHEAR_10_Oken SUBJECT_SAMPLE_FACTORS N/A HHEAR_11_Oken Study type:HHEAR reference material RAW_FILE_NAME=HHEAR_11_Oken SUBJECT_SAMPLE_FACTORS N/A HHEAR_12_Oken Study type:HHEAR reference material RAW_FILE_NAME=HHEAR_12_Oken SUBJECT_SAMPLE_FACTORS N/A HHEAR_13_Oken Study type:HHEAR reference material RAW_FILE_NAME=HHEAR_13_Oken SUBJECT_SAMPLE_FACTORS N/A HHEAR_14_Oken Study type:HHEAR reference material RAW_FILE_NAME=HHEAR_14_Oken 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sample RAW_FILE_NAME=S_31_Oken SUBJECT_SAMPLE_FACTORS EM-2FTH2-CBP-01-12701 S_32_Oken Study type:Study sample RAW_FILE_NAME=S_32_Oken SUBJECT_SAMPLE_FACTORS EM-2FMP1-CBP-01-12701 S_33_Oken Study type:Study sample RAW_FILE_NAME=S_33_Oken SUBJECT_SAMPLE_FACTORS EM-2FNS3-CBP-01-12701 S_34_Oken Study type:Study sample RAW_FILE_NAME=S_34_Oken SUBJECT_SAMPLE_FACTORS EM-2FTY4-CBP-01-12701 S_35_Oken Study type:Study sample RAW_FILE_NAME=S_35_Oken SUBJECT_SAMPLE_FACTORS EM-2FP65-CBP-01-12701 S_36_Oken Study type:Study sample RAW_FILE_NAME=S_36_Oken SUBJECT_SAMPLE_FACTORS EM-2FQV4-CBP-01-12701 S_37_Oken Study type:Study sample RAW_FILE_NAME=S_37_Oken SUBJECT_SAMPLE_FACTORS EM-2FQ31-CBP-01-12701 S_38_Oken Study type:Study sample RAW_FILE_NAME=S_38_Oken SUBJECT_SAMPLE_FACTORS EM-2FMN5-CBP-01-12701 S_39_Oken Study type:Study sample RAW_FILE_NAME=S_39_Oken SUBJECT_SAMPLE_FACTORS EM-2FPJ1-CBP-01-12701 S_3_Oken Study type:Study sample RAW_FILE_NAME=S_3_Oken SUBJECT_SAMPLE_FACTORS EM-2FRW1-CBP-01-12701 S_40_Oken Study type:Study sample RAW_FILE_NAME=S_40_Oken SUBJECT_SAMPLE_FACTORS EM-2FQK8-CBP-01-12701 S_41_Oken Study type:Study sample RAW_FILE_NAME=S_41_Oken SUBJECT_SAMPLE_FACTORS EM-2FPF0-CBP-01-12701 S_42_Oken Study type:Study sample RAW_FILE_NAME=S_42_Oken SUBJECT_SAMPLE_FACTORS EM-2FS54-CBP-01-12701 S_43_Oken Study type:Study sample RAW_FILE_NAME=S_43_Oken SUBJECT_SAMPLE_FACTORS EM-2FN59-CBP-01-12701 S_44_Oken Study type:Study sample RAW_FILE_NAME=S_44_Oken SUBJECT_SAMPLE_FACTORS EM-2FNG0-CBP-01-12701 S_45_Oken Study type:Study sample RAW_FILE_NAME=S_45_Oken SUBJECT_SAMPLE_FACTORS EM-2FSG5-CBP-01-12701 S_46_Oken Study type:Study sample RAW_FILE_NAME=S_46_Oken SUBJECT_SAMPLE_FACTORS EM-2FTA7-CBP-01-12701 S_47_Oken Study type:Study sample RAW_FILE_NAME=S_47_Oken SUBJECT_SAMPLE_FACTORS EM-2FQ49-CBP-01-12701 S_48_Oken Study type:Study sample RAW_FILE_NAME=S_48_Oken SUBJECT_SAMPLE_FACTORS EM-2FQ15-CBP-01-12701 S_49_Oken Study type:Study sample RAW_FILE_NAME=S_49_Oken SUBJECT_SAMPLE_FACTORS EM-2FR22-CBP-01-12701 S_4_Oken Study type:Study sample RAW_FILE_NAME=S_4_Oken SUBJECT_SAMPLE_FACTORS EM-2FNA3-CBP-01-12701 S_50_Oken Study type:Study sample RAW_FILE_NAME=S_50_Oken SUBJECT_SAMPLE_FACTORS EM-2FND6-CBP-01-12701 S_51_Oken Study type:Study sample RAW_FILE_NAME=S_51_Oken SUBJECT_SAMPLE_FACTORS EM-2FR48-CBP-01-12701 S_52_Oken Study type:Study sample RAW_FILE_NAME=S_52_Oken SUBJECT_SAMPLE_FACTORS EM-2FPL7-CBP-01-12701 S_53_Oken Study type:Study sample RAW_FILE_NAME=S_53_Oken SUBJECT_SAMPLE_FACTORS EM-2FMV8-CBP-01-12701 S_54_Oken Study type:Study sample RAW_FILE_NAME=S_54_Oken SUBJECT_SAMPLE_FACTORS EM-2FTK5-CBP-01-12701 S_55_Oken Study type:Study sample RAW_FILE_NAME=S_55_Oken SUBJECT_SAMPLE_FACTORS EM-2FP81-CBP-01-12701 S_56_Oken Study type:Study sample RAW_FILE_NAME=S_56_Oken SUBJECT_SAMPLE_FACTORS EM-2FPX0-CBP-01-12701 S_57_Oken Study type:Study sample RAW_FILE_NAME=S_57_Oken SUBJECT_SAMPLE_FACTORS EM-2FMF3-CBP-01-12701 S_58_Oken Study type:Study sample RAW_FILE_NAME=S_58_Oken SUBJECT_SAMPLE_FACTORS EM-2FQ64-CBP-01-12701 S_59_Oken Study type:Study sample RAW_FILE_NAME=S_59_Oken SUBJECT_SAMPLE_FACTORS EM-2FTP4-CBP-01-12701 S_5_Oken Study type:Study sample RAW_FILE_NAME=S_5_Oken SUBJECT_SAMPLE_FACTORS EM-2FTE8-CBP-01-12701 S_60_Oken Study type:Study sample RAW_FILE_NAME=S_60_Oken SUBJECT_SAMPLE_FACTORS EM-2FRS9-CBP-01-12701 S_61_Oken Study type:Study sample RAW_FILE_NAME=S_61_Oken SUBJECT_SAMPLE_FACTORS EM-2FQJ0-CBP-01-12701 S_62_Oken Study type:Study sample RAW_FILE_NAME=S_62_Oken SUBJECT_SAMPLE_FACTORS EM-2FT61-CBP-01-12701 S_63_Oken Study type:Study sample RAW_FILE_NAME=S_63_Oken SUBJECT_SAMPLE_FACTORS EM-2FpS1-CBP-01-12701 S_64_Oken Study type:Study sample RAW_FILE_NAME=S_64_Oken SUBJECT_SAMPLE_FACTORS EM-2FNQ8-CBP-01-12701 S_65_Oken Study type:Study sample RAW_FILE_NAME=S_65_Oken SUBJECT_SAMPLE_FACTORS EM-2FML0-CBP-01-12701 S_66_Oken Study type:Study sample RAW_FILE_NAME=S_66_Oken SUBJECT_SAMPLE_FACTORS EM-2FTW9-CBP-01-12701 S_67_Oken Study type:Study sample RAW_FILE_NAME=S_67_Oken SUBJECT_SAMPLE_FACTORS EM-2FTD0-CBP-01-12701 S_68_Oken Study type:Study sample RAW_FILE_NAME=S_68_Oken SUBJECT_SAMPLE_FACTORS EM-2FTT5-CBP-01-12701 S_69_Oken Study type:Study sample RAW_FILE_NAME=S_69_Oken SUBJECT_SAMPLE_FACTORS EM-2FQ80-CBP-01-12701 S_6_Oken Study type:Study sample RAW_FILE_NAME=S_6_Oken SUBJECT_SAMPLE_FACTORS EM-2FSY5-CBP-01-12701 S_70_Oken Study type:Study sample RAW_FILE_NAME=S_70_Oken SUBJECT_SAMPLE_FACTORS EM-2FS62-CBP-01-12701 S_71_Oken Study type:Study sample RAW_FILE_NAME=S_71_Oken SUBJECT_SAMPLE_FACTORS EM-2FQ56-CBP-01-12701 S_72_Oken Study type:Study sample RAW_FILE_NAME=S_72_Oken SUBJECT_SAMPLE_FACTORS EM-2FNT1-CBP-01-12701 S_73_Oken Study type:Study sample RAW_FILE_NAME=S_73_Oken SUBJECT_SAMPLE_FACTORS EM-2FNN4-CBP-01-12701 S_74_Oken Study type:Study sample RAW_FILE_NAME=S_74_Oken SUBJECT_SAMPLE_FACTORS EM-2FPD4-CBP-01-12701 S_75_Oken Study type:Study sample RAW_FILE_NAME=S_75_Oken SUBJECT_SAMPLE_FACTORS EM-2FPN2-CBP-01-12701 S_76_Oken Study type:Study sample RAW_FILE_NAME=S_76_Oken SUBJECT_SAMPLE_FACTORS EM-2FRQ4-CBP-01-12701 S_77_Oken Study type:Study sample RAW_FILE_NAME=S_77_Oken SUBJECT_SAMPLE_FACTORS EM-2FTX6-CBP-01-12701 S_78_Oken Study type:Study sample RAW_FILE_NAME=S_78_Oken SUBJECT_SAMPLE_FACTORS EM-2FRP6-CBP-01-12701 S_79_Oken Study type:Study sample RAW_FILE_NAME=S_79_Oken SUBJECT_SAMPLE_FACTORS EM-2FRV3-CBP-01-12701 S_7_Oken Study type:Study sample RAW_FILE_NAME=S_7_Oken SUBJECT_SAMPLE_FACTORS EM-2FTB5-CBP-01-12701 S_80_Oken Study type:Study sample RAW_FILE_NAME=S_80_Oken SUBJECT_SAMPLE_FACTORS EM-2FNU9-CBP-01-12701 S_81_Oken Study type:Study sample RAW_FILE_NAME=S_81_Oken SUBJECT_SAMPLE_FACTORS EM-2FQR3-CBP-01-12701 S_82_Oken Study type:Study sample RAW_FILE_NAME=S_82_Oken SUBJECT_SAMPLE_FACTORS EM-2FRG6-CBP-01-12701 S_83_Oken Study type:Study sample RAW_FILE_NAME=S_83_Oken SUBJECT_SAMPLE_FACTORS EM-2FQ98-CBP-01-12701 S_84_Oken Study type:Study sample RAW_FILE_NAME=S_84_Oken SUBJECT_SAMPLE_FACTORS EM-2FR89-CBP-01-12701 S_85_Oken Study type:Study sample RAW_FILE_NAME=S_85_Oken SUBJECT_SAMPLE_FACTORS EM-2FPW3-CBP-01-12701 S_86_Oken Study type:Study sample RAW_FILE_NAME=S_86_Oken SUBJECT_SAMPLE_FACTORS EM-2FQQ5-CBP-01-12701 S_87_Oken Study type:Study sample RAW_FILE_NAME=S_87_Oken SUBJECT_SAMPLE_FACTORS EM-2FN83-CBP-01-12701 S_88_Oken Study type:Study sample RAW_FILE_NAME=S_88_Oken SUBJECT_SAMPLE_FACTORS EM-2FMG1-CBP-01-12701 S_89_Oken Study type:Study sample RAW_FILE_NAME=S_89_Oken SUBJECT_SAMPLE_FACTORS EM-2FSK6-CBP-01-12701 S_8_Oken Study type:Study sample RAW_FILE_NAME=S_8_Oken SUBJECT_SAMPLE_FACTORS EM-2FN00-CBP-01-12701 S_90_Oken Study type:Study sample RAW_FILE_NAME=S_90_Oken SUBJECT_SAMPLE_FACTORS EM-2FNH8-CBP-01-12701 S_91_Oken Study type:Study sample RAW_FILE_NAME=S_91_Oken SUBJECT_SAMPLE_FACTORS EM-2FRA9-CBP-01-12701 S_92_Oken Study type:Study sample RAW_FILE_NAME=S_92_Oken SUBJECT_SAMPLE_FACTORS EM-2FRD2-CBP-01-12701 S_93_Oken Study type:Study sample RAW_FILE_NAME=S_93_Oken SUBJECT_SAMPLE_FACTORS EM-2FNE4-CBP-01-12701 S_94_Oken Study type:Study sample RAW_FILE_NAME=S_94_Oken SUBJECT_SAMPLE_FACTORS EM-2FT46-CBP-01-12701 S_95_Oken Study type:Study sample RAW_FILE_NAME=S_95_Oken SUBJECT_SAMPLE_FACTORS EM-2FPE2-CBP-01-12701 S_96_Oken Study type:Study sample RAW_FILE_NAME=S_96_Oken SUBJECT_SAMPLE_FACTORS EM-2FR71-CBP-01-12701 S_97_Oken Study type:Study sample RAW_FILE_NAME=S_97_Oken SUBJECT_SAMPLE_FACTORS EM-2FPM5-CBP-01-12701 S_98_Oken Study type:Study sample RAW_FILE_NAME=S_98_Oken SUBJECT_SAMPLE_FACTORS EM-2FSL4-CBP-01-12701 S_99_Oken Study type:Study sample RAW_FILE_NAME=S_99_Oken SUBJECT_SAMPLE_FACTORS EM-2FT79-CBP-01-12701 S_9_Oken Study type:Study sample RAW_FILE_NAME=S_9_Oken SUBJECT_SAMPLE_FACTORS EM-2FMY1-QCSP-10-12701 SP_10_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_10_Oken SUBJECT_SAMPLE_FACTORS EM-2FN42-QCSP-11-12701 SP_11_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_11_Oken SUBJECT_SAMPLE_FACTORS EM-2FS13-QCSP-12-12701 SP_12_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_12_Oken SUBJECT_SAMPLE_FACTORS EM-2FT20-QCSP-13-12701 SP_13_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_13_Oken SUBJECT_SAMPLE_FACTORS EM-2FR55-QCSP-14-12701 SP_14_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_14_Oken SUBJECT_SAMPLE_FACTORS EM-2FSR1-QCSP-15-12701 SP_15_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_15_Oken SUBJECT_SAMPLE_FACTORS EM-2FTM1-QCSP-16-12701 SP_16_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_16_Oken SUBJECT_SAMPLE_FACTORS EM-2FSC4-QCSP-17-12701 SP_17_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_17_Oken SUBJECT_SAMPLE_FACTORS EM-2FMA4-QCSP-18-12701 SP_18_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_18_Oken SUBJECT_SAMPLE_FACTORS EM-2FME5-QCSP-19-12701 SP_19_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_19_Oken SUBJECT_SAMPLE_FACTORS EM-2FQL6-QCSP-01-12701 SP_1_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_1_Oken SUBJECT_SAMPLE_FACTORS EM-2FQM4-QCSP-20-12701 SP_20_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_20_Oken SUBJECT_SAMPLE_FACTORS EM-2FSN9-QCSP-02-12701 SP_2_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_2_Oken SUBJECT_SAMPLE_FACTORS EM-2FNL9-QCSP-03-12701 SP_3_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_3_Oken SUBJECT_SAMPLE_FACTORS EM-2FSD1-QCSP-04-12701 SP_4_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_4_Oken SUBJECT_SAMPLE_FACTORS EM-2FR06-QCSP-05-12701 SP_5_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_5_Oken SUBJECT_SAMPLE_FACTORS EM-2FS47-QCSP-06-12701 SP_6_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_6_Oken SUBJECT_SAMPLE_FACTORS EM-2FSU4-QCSP-07-12701 SP_7_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_7_Oken SUBJECT_SAMPLE_FACTORS EM-2FRH4-QCSP-08-12701 SP_8_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_8_Oken SUBJECT_SAMPLE_FACTORS EM-2FN18-QCSP-09-12701 SP_9_Oken Study type:Total Study Pool RAW_FILE_NAME=SP_9_Oken #COLLECTION CO:COLLECTION_SUMMARY Pregnant women were enrolled in Project Viva between 1999 and 2002 at their CO:COLLECTION_SUMMARY first prenatal visit at one of 8 obstetric clinics of Atrius Harvard Vanguard CO:COLLECTION_SUMMARY Medical Associates, a multispecialty group practice in eastern Massachusetts. CO:COLLECTION_SUMMARY Eligible mothers were fluent in English, had singleton gestations, were <22 CO:COLLECTION_SUMMARY weeks gestation, and had no plans to move away from the study area. Research CO:COLLECTION_SUMMARY staff performed in-person study visits with participating mothers in the first CO:COLLECTION_SUMMARY (median gestational age 9.9 weeks) and second (median gestational age 28.1 CO:COLLECTION_SUMMARY weeks) trimesters of pregnancy, and with mothers and children during the first CO:COLLECTION_SUMMARY few days after delivery, during infancy (median age 6.3 months), in early CO:COLLECTION_SUMMARY childhood (median age 3.3 years), mid-childhood (median age 7.7 years), and CO:COLLECTION_SUMMARY adolescence (median age13 years). CO:SAMPLE_TYPE Blood (plasma) CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY N/A #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Study plasma samples (50 µL) and total study pool samples (50 µL) were SP:SAMPLEPREP_SUMMARY prepared and shipped from the Channing Laboratory Division of Network Medicine SP:SAMPLEPREP_SUMMARY at Brigham and Women’s Hospital, Boston, MA, to the NC HHEAR Hub on dry ice. SP:SAMPLEPREP_SUMMARY Aliquots of HHEAR reference plasma (50 µL each) and NIST plasma (1950) SP:SAMPLEPREP_SUMMARY reference material (50 µL each) were provided by the NC HHEAR Hub. All samples SP:SAMPLEPREP_SUMMARY were stored under -80° C until lab analysis. All samples were thawed at 4 °C SP:SAMPLEPREP_SUMMARY overnight a day before sample preparation. The same volume (50 µL) of LC-MS SP:SAMPLEPREP_SUMMARY grade water was used as blank. Samples, including study samples, total study SP:SAMPLEPREP_SUMMARY pool samples, HHEAR reference material, NIST reference material, and blanks were SP:SAMPLEPREP_SUMMARY mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal SP:SAMPLEPREP_SUMMARY standard and vortexed by the multiple tube vortex mixer for 2 min at 5000 rpm in SP:SAMPLEPREP_SUMMARY room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. SP:SAMPLEPREP_SUMMARY The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind SP:SAMPLEPREP_SUMMARY Eppendorf tube, and then dried by a SpeedVac overnight. For immediate analysis, SP:SAMPLEPREP_SUMMARY 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the SP:SAMPLEPREP_SUMMARY dried extracts, and the samples were thoroughly mixed on the multiple tube SP:SAMPLEPREP_SUMMARY vortex mixer for 10 min at 5000 rpm in room temperature. All samples were SP:SAMPLEPREP_SUMMARY centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to SP:SAMPLEPREP_SUMMARY pre-labeled autosampler vials for data acquisition by LC-MS. SP:PROCESSING_STORAGE_CONDITIONS On ice SP:EXTRACT_STORAGE Described in summary #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Reverse phase CH:INSTRUMENT_NAME Thermo Scientific™ Vanquish™ UPHPLC CH:COLUMN_NAME Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um) CH:COLUMN_PRESSURE 6000-10000 CH:COLUMN_TEMPERATURE 50 CH:FLOW_GRADIENT Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. CH:FLOW_GRADIENT 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 CH:FLOW_GRADIENT 0.4 99.0 1.0 5 CH:FLOW_RATE 0.4 ml/min CH:INJECTION_TEMPERATURE 8 CH:SOLVENT_A 100% water; 0.1% formic acid CH:SOLVENT_B 100% methanol; 0.1% formic acid CH:RANDOMIZATION_ORDER Randomized CH:CHROMATOGRAPHY_TYPE Reversed phase #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Q Exactive HF hybrid Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:MS_COMMENTS Instrument: Thermo Q Exactive HFx Software: Xcalibur 4.1.31.9 for data MS:MS_COMMENTS acquisition; Progenesis QI 2.1 for data preprocessing MS:ION_MODE POSITIVE MS:CAPILLARY_TEMPERATURE 320 °C MS:CAPILLARY_VOLTAGE 3.5 KV MS:COLLISION_ENERGY 20-45, ramp MS:COLLISION_GAS N2 MS:DRY_GAS_FLOW 55 MS:DRY_GAS_TEMP 400°C MS:FRAGMENTATION_METHOD CID MS:DESOLVATION_GAS_FLOW 55 MS:MS_RESULTS_FILE ST002050_AN003337_Results.txt UNITS:Normalized intensity Has m/z:Yes Has RT:Yes RT units:Minutes #END