#METABOLOMICS WORKBENCH joerghofmann_20220406_110456 DATATRACK_ID:3177 STUDY_ID:ST002140 ANALYSIS_ID:AN003502 PROJECT_ID:PR001355
VERSION             	1
CREATED_ON             	April 15, 2022, 1:34 pm
#PROJECT
PR:PROJECT_TITLE                 	Mitochondrial respiration in B lymphocytes is essential for humoral immunity by
PR:PROJECT_TITLE                 	controlling flux of the TCA cycle
PR:PROJECT_SUMMARY               	The function of mitochondrial respiration during B cell fate decisions and
PR:PROJECT_SUMMARY               	differentiation 55 remained equivocal. This study reveals that selection for
PR:PROJECT_SUMMARY               	mitochondrial fitness occurs during B 56 cell activation and is essential for
PR:PROJECT_SUMMARY               	subsequent plasma cell differentiation. By expressing a 57 mutated mitochondrial
PR:PROJECT_SUMMARY               	helicase in transitional B cells, we depleted mitochondrial DNA during 58 B cell
PR:PROJECT_SUMMARY               	maturation, resulting in reduced oxidative phosphorylation. Although no changes
PR:PROJECT_SUMMARY               	in 59 follicular B cell development were evident, germinal centers, class switch
PR:PROJECT_SUMMARY               	recombination to 60 IgG, plasma cell maturation and humoral immunity were
PR:PROJECT_SUMMARY               	diminished. Defective oxidative 61 phosphorylation led to aberrant flux of the
PR:PROJECT_SUMMARY               	tricarboxylic acid cycle and lowered the amount of 62 saturated phosphatidic
PR:PROJECT_SUMMARY               	acid. Consequently, mTOR activity and BLIMP1 induction were 63 curtailed whereas
PR:PROJECT_SUMMARY               	HIF1 _and glycolysis were amplified. Exogenous phosphatidic acid 64 increased
PR:PROJECT_SUMMARY               	mTOR activity in activated B cells. Hence, mitochondrial function is required
PR:PROJECT_SUMMARY               	and 65 selected for in activated B cells for the successful generation of
PR:PROJECT_SUMMARY               	functional plasma cells.
PR:INSTITUTE                     	University of Erlangen-Nuremberg Chair of Biochemistry
PR:LAST_NAME                     	Hofmann
PR:FIRST_NAME                    	Joerg
PR:ADDRESS                       	Staudtstr.5, Erlangen, Bavaria, 91058, Germany
PR:EMAIL                         	joerg.hofmann@fau.de
PR:PHONE                         	+49 9131 85 8260
#STUDY
ST:STUDY_TITLE                   	Mitochondrial respiration in B lymphocytes is essential for humoral immunity by
ST:STUDY_TITLE                   	controlling flux of the TCA cycle
ST:STUDY_SUMMARY                 	The function of mitochondrial respiration during B cell fate decisions and
ST:STUDY_SUMMARY                 	differentiation 55 remained equivocal. This study reveals that selection for
ST:STUDY_SUMMARY                 	mitochondrial fitness occurs during B 56 cell activation and is essential for
ST:STUDY_SUMMARY                 	subsequent plasma cell differentiation. By expressing a 57 mutated mitochondrial
ST:STUDY_SUMMARY                 	helicase in transitional B cells, we depleted mitochondrial DNA during 58 B cell
ST:STUDY_SUMMARY                 	maturation, resulting in reduced oxidative phosphorylation. Although no changes
ST:STUDY_SUMMARY                 	in 59 follicular B cell development were evident, germinal centers, class switch
ST:STUDY_SUMMARY                 	recombination to 60 IgG, plasma cell maturation and humoral immunity were
ST:STUDY_SUMMARY                 	diminished. Defective oxidative 61 phosphorylation led to aberrant flux of the
ST:STUDY_SUMMARY                 	tricarboxylic acid cycle and lowered the amount of 62 saturated phosphatidic
ST:STUDY_SUMMARY                 	acid. Consequently, mTOR activity and BLIMP1 induction were 63 curtailed whereas
ST:STUDY_SUMMARY                 	HIF1 _and glycolysis were amplified. Exogenous phosphatidic acid 64 increased
ST:STUDY_SUMMARY                 	mTOR activity in activated B cells. Hence, mitochondrial function is required
ST:STUDY_SUMMARY                 	and 65 selected for in activated B cells for the successful generation of
ST:STUDY_SUMMARY                 	functional plasma cells.
ST:INSTITUTE                     	University of Erlangen-Nürnberg
ST:DEPARTMENT                    	Division of Molecular Immunology.Universitätsklinikum Erlangen, Nikolaus
ST:DEPARTMENT                    	Fibinger Zentrum
ST:LABORATORY                    	Prof. Mielenz
ST:LAST_NAME                     	Mielenz
ST:FIRST_NAME                    	Dirk
ST:ADDRESS                       	Nikolaus-Fiebiger-Zentrum, Glückstraße 6, 91054 Erlangen
ST:EMAIL                         	dirk.mielenz@fau.de
ST:PHONE                         	++49 9131 8539105
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENDER                        	Male and female
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	1aCre	Genotype:wildtype	Treatment=Crecontrol; RAW_FILE_NAME=1aCre_191204_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS           	-	2aCre	Genotype:wildtype	Treatment=Crecontrol; RAW_FILE_NAME=2aCre_191204_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS           	-	3aDNT	Genotype:mutant	Treatment=DNTwinkle; RAW_FILE_NAME=3aDNT_191204_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS           	-	4aDNT	Genotype:mutant	Treatment=DNTwinkle; RAW_FILE_NAME=4aDNT_191204_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS           	-	1bCre	Genotype:wildtype	Treatment=Crecontrol; RAW_FILE_NAME=1bCre_200312_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS           	-	2bCre	Genotype:wildtype	Treatment=Crecontrol; RAW_FILE_NAME=2bCre_200312_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS           	-	3bDNT	Genotype:mutant	Treatment=DNTwinkle; RAW_FILE_NAME=3bDNT_200312_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS           	-	4bDNT	Genotype:mutant	Treatment=DNTwinkle; RAW_FILE_NAME=4bDNT_200312_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS           	-	1cCre	Genotype:wildtype	Treatment=Crecontrol; RAW_FILE_NAME=1cCre_210706_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS           	-	2cCre	Genotype:wildtype	Treatment=Crecontrol; RAW_FILE_NAME=2cCre_210706_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS           	-	3cCre	Genotype:wildtype	Treatment=Crecontrol; RAW_FILE_NAME=3cCre_210706_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS           	-	4cDNT	Genotype:mutant	Treatment=DNTwinkle; RAW_FILE_NAME=4cDNT_210706_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS           	-	5cDNT	Genotype:mutant	Treatment=DNTwinkle; RAW_FILE_NAME=5cDNT_210706_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS           	-	6cDNT	Genotype:mutant	Treatment=DNTwinkle; RAW_FILE_NAME=6cDNT_210706_PMet_BZellen.mzML
SUBJECT_SAMPLE_FACTORS           	-	B cells_DNT_GPL analysis 1-S01_DNT	Genotype:mutant	Treatment=DNTwinkle; RAW_FILE_NAME=B cells_DNT_GPL analysis 1-S01_DNT.mzML
SUBJECT_SAMPLE_FACTORS           	-	B cells_DNT_GPL analysis 1-S02_Cre	Genotype:wildtype	Treatment=Crecontrol; RAW_FILE_NAME=B cells_DNT_GPL analysis 1-S02_Cre.mzML
SUBJECT_SAMPLE_FACTORS           	-	B cells_DNT_GPL analysis 1-S03_DNT	Genotype:mutant	Treatment=DNTwinkle; RAW_FILE_NAME=B cells_DNT_GPL analysis 1-S03_DNT.mzML
SUBJECT_SAMPLE_FACTORS           	-	B cells_DNT_GPL analysis 1-S04_Cre	Genotype:wildtype	Treatment=Crecontrol; RAW_FILE_NAME=B cells_DNT_GPL analysis 1-S04_Cre.mzML
SUBJECT_SAMPLE_FACTORS           	-	B cells_DNT_GPL analysis 1-S05_DNT	Genotype:mutant	Treatment=DNTwinkle; RAW_FILE_NAME=B cells_DNT_GPL analysis 1-S05_DNT.mzML
SUBJECT_SAMPLE_FACTORS           	-	B cells_DNT_GPL analysis 1-S06_Cre	Genotype:wildtype	Treatment=Crecontrol; RAW_FILE_NAME=B cells_DNT_GPL analysis 1-S06_Cre.mzML
SUBJECT_SAMPLE_FACTORS           	-	B cells_DNT_GPL analysis 2b-S01_Cre1	Genotype:wildtype	Treatment=Crecontrol; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S01_Cre1.mzML
SUBJECT_SAMPLE_FACTORS           	-	B cells_DNT_GPL analysis 2b-S02_DNT2	Genotype:mutant	Treatment=DNTwinkle; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S02_DNT2.mzML
SUBJECT_SAMPLE_FACTORS           	-	B cells_DNT_GPL analysis 2b-S03_Cre3	Genotype:wildtype	Treatment=Crecontrol; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S03_Cre3.mzML
SUBJECT_SAMPLE_FACTORS           	-	B cells_DNT_GPL analysis 2b-S04_DNT4	Genotype:mutant	Treatment=DNTwinkle; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S04_DNT4.mzML
SUBJECT_SAMPLE_FACTORS           	-	B cells_DNT_GPL analysis 2b-S05_Cre5	Genotype:wildtype	Treatment=Crecontrol; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S05_Cre5.mzML
SUBJECT_SAMPLE_FACTORS           	-	B cells_DNT_GPL analysis 2b-S06_DNT6	Genotype:mutant	Treatment=DNTwinkle; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S06_DNT6.mzML
SUBJECT_SAMPLE_FACTORS           	-	B cells_DNT_GPL analysis 2b-S07_Cre7	Genotype:wildtype	Treatment=Crecontrol; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S07_Cre7.mzML
SUBJECT_SAMPLE_FACTORS           	-	B cells_DNT_GPL analysis 2b-S08_DNT8	Genotype:mutant	Treatment=DNTwinkle; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S08_DNT8.mzML
SUBJECT_SAMPLE_FACTORS           	-	B cells_DNT_GPL analysis 2b-S10_DNT10	Genotype:mutant	Treatment=DNTwinkle; RAW_FILE_NAME=B cells_DNT_GPL analysis 2b-S10_DNT10.mzML
#COLLECTION
CO:COLLECTION_SUMMARY            	Both female and male mice were used in the experiments. Mice were maintained on
CO:COLLECTION_SUMMARY            	a 12-h light/dark cycle with free access to food and water according to
CO:COLLECTION_SUMMARY            	governmental rules. K320E-TWINKLE floxed mice (Baris et al., 2015) were crossed
CO:COLLECTION_SUMMARY            	to CD23 CRE mice (Kwon et al., 2008) kindly provided by Meinrad Busslinger) to
CO:COLLECTION_SUMMARY            	generate DNT animals. DNT mice used in these experiments had the genetic
CO:COLLECTION_SUMMARY            	background DNT+/- CRE+/- and CRE control mice were DNT-/- CRE+/-. The WT animals
CO:COLLECTION_SUMMARY            	used in this study were DNT-/- CRE-/- littermates. All mice were on the C57Bl/6
CO:COLLECTION_SUMMARY            	background. Isolation of primary murine cells from spleen and bone marrow Spleen
CO:COLLECTION_SUMMARY            	was transferred into cold 2 % FCS (in PBS) and gently passed through a 70 µm
CO:COLLECTION_SUMMARY            	cell strainer (BD) using the plunger of a 5 ml syringe (BD). Femur and tibia
CO:COLLECTION_SUMMARY            	were flushed with cold 2 % FCS using a 27 G cannula (BD). Cell suspensions were
CO:COLLECTION_SUMMARY            	pelleted by centrifugation at 300 x g for 5 min at 4°C. Erythrocytes were lysed
CO:COLLECTION_SUMMARY            	in red blood cell-lysis buffer (150 mM NH4Cl, 10 mM KHCO3, 100 µM EDTA) for
CO:COLLECTION_SUMMARY            	5min at room temperature. The reaction was stopped by adding cold 2% FCS before
CO:COLLECTION_SUMMARY            	centrifugation at 300 x g for 5 min at 4°C. The final cell suspensions were
CO:COLLECTION_SUMMARY            	kept in cold 2 % FCS after filtration through 30 µm mesh filter (Sysmex). In
CO:COLLECTION_SUMMARY            	vitro cultivation of primary murine B cells Splenic B cells were cultured with a
CO:COLLECTION_SUMMARY            	starting concentration of 0.5 x 106 cells/ ml in R10 medium (RPMI1640, 10 %
CO:COLLECTION_SUMMARY            	fetal calf serum (FCS), 2 mM glutamate, 1 mM sodium pyruvate, 50 U/ml penicillin
CO:COLLECTION_SUMMARY            	G, 50 μg/ml streptomycin, 50 μM β-mercaptoethanol) for 72 h at 37°C and 5%
CO:COLLECTION_SUMMARY            	CO2, supplemented with 10 µg/ml LPS. For in vitro class switch recombination
CO:COLLECTION_SUMMARY            	cells were seeded at 0.1 x 106 cells/ ml in R10 medium for 96 h, supplemented
CO:COLLECTION_SUMMARY            	with 5 ng/ml transforming growth factor , 5 nM retinoic acid, 10 µg/ml
CO:COLLECTION_SUMMARY            	anti-CD40 antibody, 10 µg/ml LPS, 100 U/ml IL4 and 10 ng/ml IL5. Ref.: Baris,
CO:COLLECTION_SUMMARY            	O.R., Ederer, S., Neuhaus, J.F., von Kleist-Retzow, J.C., Wunderlich, C.M., Pal,
CO:COLLECTION_SUMMARY            	M., WunderlichF.T., Peeva, V., Zsurka, G., Kunz, W.S., et al. (2015). Mosaic
CO:COLLECTION_SUMMARY            	Deficiency in Mitochondrial Oxidative Metabolism Promotes Cardiac Arrhythmia
CO:COLLECTION_SUMMARY            	during Aging. Cell Metab 21, 667–677.
CO:COLLECTION_PROTOCOL_FILENAME  	Bcellscoll Mielenz.pdf
CO:SAMPLE_TYPE                   	B-cells
#TREATMENT
TR:TREATMENT_SUMMARY             	in vitro activated (LPS) B cells, which cannot replicate their mitochondrial DNA
TR:TREATMENT_SUMMARY             	("DNTwinkle") resulting in impaired respiratory chain activity and oxidative
TR:TREATMENT_SUMMARY             	phosphorylation compared to Cre control B cells DTN Dominant Negative Twinkle
TR:TREATMENT_SUMMARY             	(mitochondtail Helicase)
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Glycerophospholipid analysis Glycerophospholipids (PCH, PE, PI, PS, PG, PA) in B
SP:SAMPLEPREP_SUMMARY            	cells were analyzed by Nano- Electrospray Ionization Tandem Mass Spectrometry
SP:SAMPLEPREP_SUMMARY            	(Nano-ESI-MS/MS) with direct infusion of the lipid extract (Shotgun Lipidomics):
SP:SAMPLEPREP_SUMMARY            	14 to 45 x 106 cells were homogenized in 300 μl of Milli- Q water using the
SP:SAMPLEPREP_SUMMARY            	Precellys 24 Homogenisator (Peqlab, Erlangen, Germany) at 6.500 rpm for 30 sec.
SP:SAMPLEPREP_SUMMARY            	The protein content of the homogenate was routinely determined using
SP:SAMPLEPREP_SUMMARY            	bicinchoninic acid. To 100 μl of the homogenate 400 μl of Milli-Q water,
SP:SAMPLEPREP_SUMMARY            	1.875ml of methanol/chloroform 2:1 (v/v) and internal standards (125 pmol PCH
SP:SAMPLEPREP_SUMMARY            	17:0-20:4, 132 pmol PE 17:0-20:4, 118 pmol PI 17:0-20:4, 131 pmol PS 17:0-20:4,
SP:SAMPLEPREP_SUMMARY            	62 pmol PG 17:0/20:4, 75 pmol PA 17:0/20:4 Avanti Polar Lipids) were added.
SP:SAMPLEPREP_SUMMARY            	Lipid extraction and Nano-ESI-MS/MS analysis were performed as previously
SP:SAMPLEPREP_SUMMARY            	described (Kumar et al., 2015). Endogenous glycerophospolipids were quantified
SP:SAMPLEPREP_SUMMARY            	by referring their peak areas to those of the internal standards. The calculated
SP:SAMPLEPREP_SUMMARY            	glycerophospolipid amounts were normalized to the protein content of the tissue
SP:SAMPLEPREP_SUMMARY            	homogenate. Metabolomics of phosphorylated metabolites und carbonic acids
SP:SAMPLEPREP_SUMMARY            	Experimental Setup I: Splenic B cells were isolated, activated with LPS and
SP:SAMPLEPREP_SUMMARY            	viable cells, only GFP+ for DNT, were sorted after 3 days using flow cytometry.
SP:SAMPLEPREP_SUMMARY            	Perchloric acid extraction and metabolic profiling was performed as previously
SP:SAMPLEPREP_SUMMARY            	published measured by LCMS/MS on an QTrap 3200 (Sciex) (Hofmann et al., 2011).
SP:SAMPLEPREP_SUMMARY            	Ref.: Kumar, V., Bouameur, J.E., Bar, J., Rice, R.H., Hornig-Do, H.T., Roop,
SP:SAMPLEPREP_SUMMARY            	D.R., Schwarz, N., Brodesser, 1120 S., Thiering, S., Leube, R.E., et al. (2015).
SP:SAMPLEPREP_SUMMARY            	A keratin scaffold regulates epidermal barrier 1121 formation, mitochondrial
SP:SAMPLEPREP_SUMMARY            	lipid composition, and activity. J Cell Biol 211, 1057–1075. Ref.: Hofmann,
SP:SAMPLEPREP_SUMMARY            	J., Bornke, F., Schmiedl, A., Kleine, T., and Sonnewald, U. (2011). Detecting
SP:SAMPLEPREP_SUMMARY            	functional groups of Arabidopsis mutants by metabolic profiling and evaluation
SP:SAMPLEPREP_SUMMARY            	of pleiotropic responses. 10Front Plant Sci 2, 82.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Dried lipid extracts were dissolved in 300 μl of methanol. 20 μl of the lipid
CH:CHROMATOGRAPHY_SUMMARY        	extract in methanol were loaded into 96-well plates and diluted with 20 μl of
CH:CHROMATOGRAPHY_SUMMARY        	20 mM ammonium acetate in methanol. Lipid infusion and ionization was conducted
CH:CHROMATOGRAPHY_SUMMARY        	using Nano-ESI chips with the TriVersa NanoMate operated by the ChipSoft
CH:CHROMATOGRAPHY_SUMMARY        	Software (Advion) under the following settings: sample infusion volume: 14 μl,
CH:CHROMATOGRAPHY_SUMMARY        	volume of air to aspirate after sample: 1 μl, air gap before chip: enabled,
CH:CHROMATOGRAPHY_SUMMARY        	aspiration delay: 0 s, prepiercing: with mandrel, spray sensing: enabled,
CH:CHROMATOGRAPHY_SUMMARY        	cooling temperature: 14°C, gas pressure: 0.5 psi, ionization voltage: 1.4 kV,
CH:CHROMATOGRAPHY_SUMMARY        	and vent headspace: enabled. Prewetting was done once. Ref.: Kumar, V.,
CH:CHROMATOGRAPHY_SUMMARY        	Bouameur, J.E., Bar, J., Rice, R.H., Hornig-Do, H.T., Roop, D.R., Schwarz, N.,
CH:CHROMATOGRAPHY_SUMMARY        	Brodesser, 1120 S., Thiering, S., Leube, R.E., et al. (2015). A keratin scaffold
CH:CHROMATOGRAPHY_SUMMARY        	regulates epidermal barrier 1121 formation, mitochondrial lipid composition, and
CH:CHROMATOGRAPHY_SUMMARY        	activity. J Cell Biol 211, 1057–1075. Ref.: Kumar, V., Bouameur, J.E., Bar,
CH:CHROMATOGRAPHY_SUMMARY        	J., Rice, R.H., Hornig-Do, H.T., Roop, D.R., Schwarz, N., Brodesser, 1120 S.,
CH:CHROMATOGRAPHY_SUMMARY        	Thiering, S., Leube, R.E., et al. (2015). A keratin scaffold regulates epidermal
CH:CHROMATOGRAPHY_SUMMARY        	barrier 1121 formation, mitochondrial lipid composition, and activity. J Cell
CH:CHROMATOGRAPHY_SUMMARY        	Biol 211, 1057–1075.
CH:CHROMATOGRAPHY_TYPE           	None (Direct infusion)
CH:INSTRUMENT_NAME               	TriVersa NanoMate
CH:COLUMN_NAME                   	TriVersa NanoMate
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	ABI Sciex 6500 QTrap
MS:INSTRUMENT_TYPE               	QTRAP
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	Mass spectrometric analysis was performed using the QTRAP 6500 (SCIEX) operated
MS:MS_COMMENTS                   	by Analyst 1.6.3. The following instrument dependent settings were used: curtain
MS:MS_COMMENTS                   	gas, 20 psi; CAD gas, medium; and interface heater temperature, 100°C. PC
MS:MS_COMMENTS                   	analysis was performed in the positive ion mode by scanning for precursors of
MS:MS_COMMENTS                   	m/z 184 at a collision energy of 35 eV. PE, PS, PG, PI, and PA measurements were
MS:MS_COMMENTS                   	performed in the positive ion mode by scanning for neutral losses of 141, 185,
MS:MS_COMMENTS                   	189, 277, and 115 D at CE of 25 eV. The value for the declustering potential was
MS:MS_COMMENTS                   	100 V (Özbalci et al. (2013) Methods Mol Biol 1033:3-20). Scanning was
MS:MS_COMMENTS                   	performed in a mass range of m/z 650–900 D and at a scan rate of 200 D/s. 61
MS:MS_COMMENTS                   	MCA spectra were accumulated. Mass spectra were processed by the LipidView
MS:MS_COMMENTS                   	Software Version 1.2 (SCIEX) for identification and quantification of
MS:MS_COMMENTS                   	glycerophospholipids. Endogenous glycerophospolipids were quantified by
MS:MS_COMMENTS                   	referring their peak areas to those of the internal standards.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	mol%
MS_METABOLITE_DATA_START
Samples	B cells_DNT_GPL analysis 1-S01_DNT	B cells_DNT_GPL analysis 1-S02_Cre	B cells_DNT_GPL analysis 1-S03_DNT	B cells_DNT_GPL analysis 1-S04_Cre	B cells_DNT_GPL analysis 1-S05_DNT	B cells_DNT_GPL analysis 1-S06_Cre	B cells_DNT_GPL analysis 2b-S01_Cre1	B cells_DNT_GPL analysis 2b-S02_DNT2	B cells_DNT_GPL analysis 2b-S03_Cre3	B cells_DNT_GPL analysis 2b-S04_DNT4	B cells_DNT_GPL analysis 2b-S05_Cre5	B cells_DNT_GPL analysis 2b-S06_DNT6	B cells_DNT_GPL analysis 2b-S07_Cre7	B cells_DNT_GPL analysis 2b-S08_DNT8	B cells_DNT_GPL analysis 2b-S10_DNT10
Factors	Genotype:mutant	Genotype:wildtype	Genotype:mutant	Genotype:wildtype	Genotype:mutant	Genotype:wildtype	Genotype:wildtype	Genotype:mutant	Genotype:wildtype	Genotype:mutant	Genotype:wildtype	Genotype:mutant	Genotype:wildtype	Genotype:mutant	Genotype:mutant
Phosphatidylcholine_30:0	5	4	5	4	5	4	3	5	3	5	3	5	3	4	5
Phosphatidylcholine_32:0	9	8	9	8	10	7	8	11	8	11	7	11	6	10	10
Phosphatidylcholine_32:1	15	14	14	16	14	15	14	15	16	14	16	15	15	14	15
Phosphatidylcholine_32:2	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
Phosphatidylcholine_32:3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylcholine_34:0	0	0	1	0	0	0	0	0	0	1	0	0	0	0	0
Phosphatidylcholine_34:1	21	22	21	23	20	23	24	22	23	21	22	20	22	21	20
Phosphatidylcholine_34:2	6	6	6	7	7	8	7	7	8	6	8	6	7	6	6
Phosphatidylcholine_34:3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylcholine_34:4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylcholine_36:0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylcholine_36:1	3	4	3	4	3	3	3	2	3	3	3	2	3	3	3
Phosphatidylcholine_36:2	8	10	9	10	8	10	10	7	10	8	11	7	11	9	8
Phosphatidylcholine_36:3	1	1	2	1	2	1	1	1	1	1	1	1	1	1	1
Phosphatidylcholine_36:4	1	1	1	1	1	1	1	1	1	1	0	1	0	1	1
Phosphatidylcholine_36:5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylcholine_36:6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylcholine_38:2	1	1	1	1	1	1	1	0	1	1	1	1	1	1	1
Phosphatidylcholine_38:3	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylcholine_38:4	1	0	1	1	1	1	0	1	0	1	0	1	0	0	1
Phosphatidylcholine_38:5	1	1	1	1	1	1	1	1	0	1	0	1	0	1	1
Phosphatidylcholine_38:6	0	0	1	0	1	0	0	1	0	0	0	1	0	0	1
Phosphatidylcholine_38:7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylcholine_40:3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylcholine_40:4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylcholine_40:5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylcholine_40:6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylcholine_40:7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylcholine_40:8	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylcholine_40:9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylethanolamine_32:0	2	1	1	1	2	1	1	2	1	2	1	2	1	1	1
Phosphatidylethanolamine_32:1	6	6	5	6	5	6	5	5	6	4	7	5	7	6	5
Phosphatidylethanolamine_34:1	11	14	10	13	10	14	14	10	15	10	16	10	17	13	11
Phosphatidylethanolamine_34:2	5	5	4	5	4	5	5	4	6	4	6	4	6	6	4
Phosphatidylethanolamine_34:3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylethanolamine_36:0	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
Phosphatidylethanolamine_36:1	3	5	3	5	3	5	5	3	5	3	6	3	6	5	3
Phosphatidylethanolamine_36:2	7	9	7	8	7	9	9	6	10	7	10	7	10	10	7
Phosphatidylethanolamine_36:3	2	2	3	2	3	2	2	3	2	3	2	3	2	3	3
Phosphatidylethanolamine_36:4	6	5	6	5	6	5	6	6	5	6	4	6	3	4	6
Phosphatidylethanolamine_36:5	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
Phosphatidylethanolamine_36:6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylethanolamine_38:0	0	1	1	1	1	1	1	1	1	1	1	1	1	1	1
Phosphatidylethanolamine_38:1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0
Phosphatidylethanolamine_38:2	0	0	0	0	0	0	1	0	0	0	0	0	1	1	0
Phosphatidylethanolamine_38:3	2	1	2	1	2	1	2	2	2	2	1	2	1	1	2
Phosphatidylethanolamine_38:4	13	11	14	11	14	10	11	15	10	14	9	14	9	10	14
Phosphatidylethanolamine_38:5	8	7	8	7	8	6	7	8	6	8	6	8	4	5	8
Phosphatidylethanolamine_38:6	5	4	5	4	5	4	4	5	4	5	3	5	3	3	5
Phosphatidylethanolamine_38:7	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0
Phosphatidylethanolamine_40:4	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
Phosphatidylethanolamine_40:5	3	3	3	3	3	3	3	3	2	3	3	3	2	2	3
Phosphatidylethanolamine_40:6	6	5	7	6	7	6	5	7	5	7	5	7	4	4	7
Phosphatidylethanolamine_40:7	3	2	3	2	3	2	2	3	2	3	2	3	1	2	3
Phosphatidylethanolamine_40:8	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylethanolamine_40:9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylinositol_32:0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
Phosphatidylinositol_32:1	1	1	1	1	1	1	1	1	2	1	1	1	1	1	1
Phosphatidylinositol_32:2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylinositol_34:1	7	10	6	9	6	10	13	8	14	9	13	7	13	9	8
Phosphatidylinositol_34:2	4	3	3	3	3	4	4	4	4	4	4	4	3	3	4
Phosphatidylinositol_34:3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylinositol_36:1	12	17	10	15	9	17	19	10	22	11	21	10	23	16	10
Phosphatidylinositol_36:2	22	23	19	22	19	24	23	19	24	20	26	19	24	24	20
Phosphatidylinositol_36:3	3	2	3	2	3	2	2	3	2	3	2	3	2	2	3
Phosphatidylinositol_36:4	3	2	3	2	3	2	2	4	2	4	2	4	2	3	4
Phosphatidylinositol_36:5	1	0	1	0	1	0	0	1	0	1	0	1	0	0	1
Phosphatidylinositol_38:1	0	1	0	1	0	1	1	0	1	0	1	0	1	1	0
Phosphatidylinositol_38:2	2	3	2	3	2	3	3	2	3	2	3	2	3	3	2
Phosphatidylinositol_38:3	6	6	7	6	8	6	6	6	4	6	5	6	5	6	7
Phosphatidylinositol_38:4	22	17	27	19	25	17	15	24	11	20	12	24	14	20	22
Phosphatidylinositol_38:5	10	7	11	8	12	7	5	11	5	9	5	11	4	7	11
Phosphatidylinositol_38:6	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
Phosphatidylinositol_40:2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylinositol_40:3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylinositol_40:5	2	2	2	2	2	2	2	2	1	2	1	2	1	2	2
Phosphatidylinositol_40:6	2	2	2	2	2	2	2	2	1	2	1	2	1	1	2
Phosphatidylinositol_40:7	1	1	1	1	1	1	0	1	0	0	0	1	0	0	1
Phosphatidylinositol_40:9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Phosphatidylserine_32:3	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0
Phosphatidylserine_36:4	1	1	1	1	1	1	2	2	1	2	2	1	1	1	1
Phosphatidylserine_38:1	1	1	1	1	1	1	1	1	2	1	1	1	2	1	1
Phosphatidylserine_38:3	5	4	6	5	5	4	6	8	6	8	6	8	3	7	8
Phosphatidylserine_38:4	5	5	5	5	5	4	6	7	6	6	6	7	4	7	7
Phosphatidylserine_38:5	3	3	3	3	3	2	3	4	3	4	3	4	3	2	4
Phosphatidylserine_38:6	3	2	2	2	2	2	2	3	2	2	2	3	2	2	3
Phosphatidylserine_40:1	0	0	0	0	0	0	1	0	1	0	1	0	0	0	0
Phosphatidylserine_40:2	0	0	0	0	0	0	1	0	1	0	1	0	1	1	0
Phosphatidylserine_40:3	0	0	0	0	0	0	0	1	1	1	1	1	0	0	1
Phosphatidylserine_40:4	4	3	4	3	4	3	4	6	3	5	4	7	4	6	6
Phosphatidylserine_40:5	16	10	15	12	15	10	13	20	13	17	13	21	13	14	19
Phosphatidylserine_40:6	19	12	18	14	18	11	14	21	14	20	13	22	15	18	21
Phosphatidylserine_40:7	2	1	2	1	2	1	1	2	1	1	1	2	2	2	2
Phosphatidylglycerol_32:0	3	3	3	3	3	3	3	4	5	5	9	3	3	6	4
Phosphatidylglycerol_32:1	5	4	4	4	5	4	6	5	3	5	5	4	4	5	5
Phosphatidylglycerol_32:2	1	1	1	1	1	1	0	1	1	1	1	1	1	1	1
Phosphatidylglycerol_34:1	65	61	60	66	61	64	52	63	59	42	52	64	66	45	62
Phosphatidylglycerol_34:2	6	5	5	5	6	5	5	7	5	4	6	5	6	6	6
Phosphatidylglycerol_36:1	6	11	8	8	7	9	16	5	9	14	11	6	8	14	6
Phosphatidylglycerol_36:2	7	7	8	6	8	7	9	7	6	10	8	8	7	11	7
Phosphatidylglycerol_36:3	2	1	2	2	2	1	1	2	1	2	2	3	2	2	2
Phosphatidylglycerol_36:4	1	1	1	1	1	1	1	2	3	3	1	2	1	2	2
Phosphatidylglycerol_38:2	1	1	1	0	1	0	1	0	1	2	1	1	1	1	1
Phosphatidylglycerol_38:3	1	1	1	1	1	0	2	1	1	4	1	1	0	2	1
Phosphatidylglycerol_38:4	1	1	1	1	1	1	2	1	2	4	1	1	1	2	1
Phosphatidylglycerol_38:5	1	1	1	0	1	0	1	1	1	2	1	1	1	1	1
Phosphatidic acid_30:1	3	1	3	1	2	1	1	2	1	1	1	2	1	1	1
Phosphatidic acid_30:2	1	0	1	0	0	0	1	1	0	1	0	1	0	0	0
Phosphatidic acid_32:0	29	38	28	36	26	35	32	17	26	11	27	17	28	14	17
Phosphatidic acid_32:1	14	7	13	8	12	8	7	11	9	9	8	11	7	8	11
Phosphatidic acid_32:2	2	1	2	2	2	1	2	2	1	1	2	2	1	2	2
Phosphatidic acid_34:0	4	10	3	8	4	9	9	4	5	3	6	3	6	3	3
Phosphatidic acid_34:1	19	20	21	20	22	22	17	21	23	20	22	22	24	26	21
Phosphatidic acid_34:2	5	5	6	6	7	5	5	8	5	5	5	7	5	6	7
Phosphatidic acid_34:3	1	0	1	0	1	0	0	1	0	1	1	1	0	1	1
Phosphatidic acid_36:0	1	1	1	1	1	1	3	2	2	2	2	2	2	2	2
Phosphatidic acid_36:1	3	4	4	4	4	4	5	6	9	10	6	7	6	10	7
Phosphatidic acid_36:2	5	5	5	5	6	6	6	8	8	11	7	8	8	10	8
Phosphatidic acid_36:3	1	1	1	1	1	1	1	2	1	2	1	2	1	2	2
Phosphatidic acid_36:4	1	0	1	0	1	0	1	2	1	2	1	2	1	1	2
Phosphatidic acid_36:5	1	0	1	0	0	0	0	1	0	1	1	1	1	1	1
Phosphatidic acid_38:2	1	1	1	1	1	1	1	1	1	2	1	1	1	1	1
Phosphatidic acid_38:4	1	0	1	0	1	0	1	2	1	3	1	2	1	2	2
Phosphatidic acid_38:5	1	0	1	1	1	1	1	2	1	2	1	2	1	1	2
Phosphatidic acid_38:6	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
Phosphatidic acid_38:7	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
Phosphatidic acid_40:5	1	0	1	0	0	0	1	3	1	4	1	2	1	2	2
Phosphatidic acid_40:6	1	0	1	0	1	0	1	2	1	4	1	3	1	2	2
Phosphatidic acid_40:7	1	1	1	1	1	1	1	1	1	1	1	1	0	1	1
Phosphatidic acid_40:8	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
Phosphatidic acid_40:9	0	0	0	0	0	0	0	1	0	1	1	0	0	0	0
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	CAS number	Mass to Charge	Fragmentation	Modification	Charge
Phosphatidylcholine_30:0	8002-43-5	706,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_32:0	8002-43-5	734,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_32:1	8002-43-5	732,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_32:2	8002-43-5	730,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_32:3	8002-43-5	728,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_34:0	8002-43-5	762,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_34:1	8002-43-5	760,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_34:2	8002-43-5	758,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_34:3	8002-43-5	756,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_34:4	8002-43-5	754,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_36:0	8002-43-5	790,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_36:1	8002-43-5	788,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_36:2	8002-43-5	786,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_36:3	8002-43-5	784,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_36:4	8002-43-5	782,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_36:5	8002-43-5	780,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_36:6	8002-43-5	778,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_38:2	8002-43-5	814,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_38:3	8002-43-5	812,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_38:4	8002-43-5	810,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_38:5	8002-43-5	808,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_38:6	8002-43-5	806,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_38:7	8002-43-5	804,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_40:3	8002-43-5	840,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_40:4	8002-43-5	838,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_40:5	8002-43-5	836,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_40:6	8002-43-5	834,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_40:7	8002-43-5	832,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_40:8	8002-43-5	830,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylcholine_40:9	8002-43-5	828,5	+Precursor Ion Scan m/z 184	[M+H]	1
Phosphatidylethanolamine_32:0	39382-08-6	692,4	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_32:1	39382-08-6	690,4	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_34:1	39382-08-6	718,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_34:2	39382-08-6	716,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_34:3	39382-08-6	714,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_36:0	39382-08-6	748,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_36:1	39382-08-6	746,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_36:2	39382-08-6	744,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_36:3	39382-08-6	742,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_36:4	39382-08-6	740,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_36:5	39382-08-6	738,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_36:6	39382-08-6	736,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_38:0	39382-08-6	776,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_38:1	39382-08-6	774,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_38:2	39382-08-6	772,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_38:3	39382-08-6	770,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_38:4	39382-08-6	768,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_38:5	39382-08-6	766,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_38:6	39382-08-6	764,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_38:7	39382-08-6	762,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_40:4	39382-08-6	796,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_40:5	39382-08-6	794,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_40:6	39382-08-6	792,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_40:7	39382-08-6	790,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_40:8	39382-08-6	788,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylethanolamine_40:9	39382-08-6	786,5	+Neutral Loss Scan m/z 141	[M+H]	1
Phosphatidylinositol_32:0	383907-36-6	828,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_32:1	383907-36-6	826,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_32:2	383907-36-6	824,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_34:1	383907-36-6	854,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_34:2	383907-36-6	852,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_34:3	383907-36-6	850,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_36:1	383907-36-6	882,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_36:2	383907-36-6	880,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_36:3	383907-36-6	878,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_36:4	383907-36-6	876,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_36:5	383907-36-6	874,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_38:1	383907-36-6	910,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_38:2	383907-36-6	908,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_38:3	383907-36-6	906,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_38:4	383907-36-6	904,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_38:5	383907-36-6	902,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_38:6	383907-36-6	900,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_40:2	383907-36-6	936,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_40:3	383907-36-6	934,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_40:5	383907-36-6	930,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_40:6	383907-36-6	928,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_40:7	383907-36-6	926,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylinositol_40:9	383907-36-6	922,5	+Neutral Loss Scan m/z 277	[M+NH4]	1
Phosphatidylserine_32:3	383907-32-2	730,5	+Neutral Loss Scan m/z 185	[M+H]	1
Phosphatidylserine_36:4	383907-32-2	784,5	+Neutral Loss Scan m/z 185	[M+H]	1
Phosphatidylserine_38:1	383907-32-2	818,5	+Neutral Loss Scan m/z 185	[M+H]	1
Phosphatidylserine_38:3	383907-32-2	814,5	+Neutral Loss Scan m/z 185	[M+H]	1
Phosphatidylserine_38:4	383907-32-2	812,5	+Neutral Loss Scan m/z 185	[M+H]	1
Phosphatidylserine_38:5	383907-32-2	810,5	+Neutral Loss Scan m/z 185	[M+H]	1
Phosphatidylserine_38:6	383907-32-2	808,5	+Neutral Loss Scan m/z 185	[M+H]	1
Phosphatidylserine_40:1	383907-32-2	846,5	+Neutral Loss Scan m/z 185	[M+H]	1
Phosphatidylserine_40:2	383907-32-2	844,5	+Neutral Loss Scan m/z 185	[M+H]	1
Phosphatidylserine_40:3	383907-32-2	842,5	+Neutral Loss Scan m/z 185	[M+H]	1
Phosphatidylserine_40:4	383907-32-2	840,5	+Neutral Loss Scan m/z 185	[M+H]	1
Phosphatidylserine_40:5	383907-32-2	838,5	+Neutral Loss Scan m/z 185	[M+H]	1
Phosphatidylserine_40:6	383907-32-2	836,5	+Neutral Loss Scan m/z 185	[M+H]	1
Phosphatidylserine_40:7	383907-32-2	834,5	+Neutral Loss Scan m/z 185	[M+H]	1
Phosphatidylglycerol_32:0	383907-64-0	740,4	+Neutral Loss Scan m/z 189	[M+NH4]	1
Phosphatidylglycerol_32:1	383907-64-0	738,4	+Neutral Loss Scan m/z 189	[M+NH4]	1
Phosphatidylglycerol_32:2	383907-64-0	736,4	+Neutral Loss Scan m/z 189	[M+NH4]	1
Phosphatidylglycerol_34:1	383907-64-0	766,4	+Neutral Loss Scan m/z 189	[M+NH4]	1
Phosphatidylglycerol_34:2	383907-64-0	764,4	+Neutral Loss Scan m/z 189	[M+NH4]	1
Phosphatidylglycerol_36:1	383907-64-0	794,5	+Neutral Loss Scan m/z 189	[M+NH4]	1
Phosphatidylglycerol_36:2	383907-64-0	792,4	+Neutral Loss Scan m/z 189	[M+NH4]	1
Phosphatidylglycerol_36:3	383907-64-0	790,4	+Neutral Loss Scan m/z 189	[M+NH4]	1
Phosphatidylglycerol_36:4	383907-64-0	788,4	+Neutral Loss Scan m/z 189	[M+NH4]	1
Phosphatidylglycerol_38:2	383907-64-0	820,5	+Neutral Loss Scan m/z 189	[M+NH4]	1
Phosphatidylglycerol_38:3	383907-64-0	818,5	+Neutral Loss Scan m/z 189	[M+NH4]	1
Phosphatidylglycerol_38:4	383907-64-0	816,5	+Neutral Loss Scan m/z 189	[M+NH4]	1
Phosphatidylglycerol_38:5	383907-64-0	814,5	+Neutral Loss Scan m/z 189	[M+NH4]	1
Phosphatidic acid_30:1	383907-53-7	636,4	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_30:2	383907-53-7	634,4	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_32:0	383907-53-7	666,4	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_32:1	383907-53-7	664,4	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_32:2	383907-53-7	662,4	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_34:0	383907-53-7	694,4	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_34:1	383907-53-7	692,4	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_34:2	383907-53-7	690,4	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_34:3	383907-53-7	688,4	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_36:0	383907-53-7	722,5	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_36:1	383907-53-7	720,5	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_36:2	383907-53-7	718,4	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_36:3	383907-53-7	716,4	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_36:4	383907-53-7	714,4	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_36:5	383907-53-7	712,4	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_38:2	383907-53-7	746,5	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_38:4	383907-53-7	742,5	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_38:5	383907-53-7	740,5	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_38:6	383907-53-7	738,5	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_38:7	383907-53-7	736,5	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_40:5	383907-53-7	768,5	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_40:6	383907-53-7	766,5	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_40:7	383907-53-7	764,5	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_40:8	383907-53-7	762,5	+Neutral Loss Scan m/z 115	[M+NH4]	1
Phosphatidic acid_40:9	383907-53-7	760,5	+Neutral Loss Scan m/z 115	[M+NH4]	1
METABOLITES_END
#END