#METABOLOMICS WORKBENCH ZJ309_20220409_201019_mwtab.txt DATATRACK_ID:3186 STUDY_ID:ST002154 ANALYSIS_ID:AN003529 PROJECT_ID:PR001367
VERSION             	1
CREATED_ON             	April 9, 2022, 8:18 pm
#PROJECT
PR:PROJECT_TITLE                 	Metabolomics Insights into the Interaction between Pseudomonas plecoglossicida
PR:PROJECT_TITLE                 	and Epinephelus coioides
PR:PROJECT_SUMMARY               	Here, a P. plecoglossicida strain (NZBD9) and economic fish of Epinephelus
PR:PROJECT_SUMMARY               	coioides were investigated as a model system to characterize pathogen-induced
PR:PROJECT_SUMMARY               	host metabolic remodeling over the course of infection. We present a nontargeted
PR:PROJECT_SUMMARY               	metabolomics profiling of E. coioides spleens from uninfected E. coioides and
PR:PROJECT_SUMMARY               	those infected with wild-type and clpV-RNA interference (RNAi) strains.
PR:INSTITUTE                     	College of Marine Food and Biological Engineering, Jimei University
PR:LABORATORY                    	Xiamen Key Laboratory of Marine Functional Food, Xiamen 361021, China
PR:LAST_NAME                     	Yang
PR:FIRST_NAME                    	Zhiqiang
PR:ADDRESS                       	No. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, China
PR:EMAIL                         	201911832016@jmu.edu.cn
PR:PHONE                         	86-592-6181487
#STUDY
ST:STUDY_TITLE                   	Virus-host interaction studies of P. plecoglossicida strain (NZBD9) and
ST:STUDY_TITLE                   	Epinephelus coioides.
ST:STUDY_SUMMARY                 	E. coioides of the infection group were intrapleurally injected with 105
ST:STUDY_SUMMARY                 	colony-forming units per fish (cfu/fish) of P. plecoglossicida (wild-type strain
ST:STUDY_SUMMARY                 	or clpV-RNAi strain), while control E. coioides received an equivalent volume of
ST:STUDY_SUMMARY                 	phosphate-buffered saline (PBS). The temperature of the water was maintained at
ST:STUDY_SUMMARY                 	18 ± 1 °C during the infection experiment. The spleens of E. coioides were
ST:STUDY_SUMMARY                 	collected at 6, 12, 24, 48, 72 and 96 hours post injection (hpi).
ST:INSTITUTE                     	Jimei University
ST:LAST_NAME                     	Yang
ST:FIRST_NAME                    	Zhiqiang
ST:ADDRESS                       	No. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, No. 185
ST:ADDRESS                       	Yinjiang Road
ST:EMAIL                         	201911832016@jmu.edu.cn
ST:PHONE                         	5926181487
#SUBJECT
SU:SUBJECT_TYPE                  	Fish
SU:SUBJECT_SPECIES               	Epinephelus coioides
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	PBS6-1-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS6-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS6-2-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS6-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS6-3-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS6-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS6-4-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS6-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS6-5-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS6-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS6-6-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS6-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS6-7-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS6-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS12-1-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS12-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS12-2-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS12-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS12-3-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS12-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS12-4-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS12-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS12-5-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS12-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS12-6-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS12-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS12-7-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS12-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS1d-1-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS1d-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS1d-2-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS1d-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS1d-3-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS1d-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS1d-4-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS1d-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS1d-5-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS1d-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS1d-6-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS1d-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS1d-7-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS1d-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS2d-1-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS2d-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS2d-2-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS2d-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS2d-3-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS2d-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS2d-4-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS2d-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS2d-5-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS2d-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS2d-6-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS2d-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS2d-7-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS2d-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS3d-1-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS3d-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS3d-2-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS3d-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS3d-3-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS3d-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS3d-4-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS3d-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS3d-5-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS3d-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS3d-6-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS3d-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS3d-7-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS3d-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS4d-1-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS4d-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS4d-2-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS4d-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS4d-3-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS4d-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS4d-4-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS4d-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS4d-5-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS4d-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS4d-6-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS4d-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS4d-7-m	Treatment:Control	Model=Positive; RAW_FILE_NAME=PBS4d-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt6-1-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt6-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt6-2-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt6-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt6-3-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt6-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt6-4-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt6-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt6-5-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt6-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt6-6-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt6-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt6-7-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt6-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt12-1-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt12-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt12-2-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt12-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt12-3-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt12-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt12-4-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt12-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt12-5-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt12-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt12-6-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt12-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt12-7-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt12-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt1d-1-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt1d-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt1d-2-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt1d-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt1d-3-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt1d-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt1d-4-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt1d-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt1d-5-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt1d-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt1d-6-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt1d-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt1d-7-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt1d-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt2d-1-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt2d-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt2d-2-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt2d-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt2d-3-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt2d-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt2d-4-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt2d-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt2d-5-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt2d-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt2d-6-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt2d-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt2d-7-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt2d-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt3d-1-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt3d-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt3d-2-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt3d-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt3d-3-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt3d-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt3d-4-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt3d-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt3d-5-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt3d-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt3d-6-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt3d-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt3d-7-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt3d-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt4d-1-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt4d-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt4d-2-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt4d-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt4d-3-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt4d-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt4d-4-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt4d-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt4d-5-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt4d-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt4d-6-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt4d-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt4d-7-m	Treatment:wild-type strain infection group	Model=Positive; RAW_FILE_NAME=wt4d-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv6-1-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv6-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv6-2-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv6-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv6-3-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv6-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv6-4-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv6-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv6-5-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv6-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv6-6-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv6-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv6-7-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv6-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv12-1-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv12-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv12-2-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv12-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv12-3-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv12-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv12-4-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv12-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv12-5-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv12-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv12-6-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv12-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv12-7-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv12-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv1d-1-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv1d-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv1d-2-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv1d-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv1d-3-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv1d-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv1d-4-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv1d-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv1d-5-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv1d-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv1d-6-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv1d-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv1d-7-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv1d-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv2d-1-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv2d-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv2d-2-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv2d-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv2d-3-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv2d-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv2d-4-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv2d-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv2d-5-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv2d-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv2d-6-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv2d-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv2d-7-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv2d-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv3d-1-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv3d-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv3d-2-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv3d-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv3d-3-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv3d-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv3d-4-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv3d-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv3d-5-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv3d-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv3d-6-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv3d-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv3d-7-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv3d-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv4d-1-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv4d-1-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv4d-2-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv4d-2-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv4d-3-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv4d-3-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv4d-4-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv4d-4-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv4d-5-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv4d-5-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv4d-6-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv4d-6-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv4d-7-m	Treatment:clpV-RNAi strain infection group	Model=Positive; RAW_FILE_NAME=clpv4d-7-m.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS6-1	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS6-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS6-2	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS6-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS6-3	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS6-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS6-4	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS6-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS6-5	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS6-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS6-6	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS6-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS6-7	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS6-7.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS12-1	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS12-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS12-2	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS12-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS12-3	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS12-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS12-4	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS12-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS12-5	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS12-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS12-6	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS12-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS12-7	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS12-7.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS1d-1	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS1d-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS1d-2	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS1d-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS1d-3	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS1d-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS1d-4	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS1d-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS1d-5	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS1d-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS1d-6	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS1d-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS1d-7	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS1d-7.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS2d-1	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS2d-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS2d-2	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS2d-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS2d-3	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS2d-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS2d-4	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS2d-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS2d-5	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS2d-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS2d-6	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS2d-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS2d-7	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS2d-7.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS3d-1	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS3d-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS3d-2	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS3d-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS3d-3	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS3d-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS3d-4	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS3d-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS3d-5	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS3d-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS3d-6	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS3d-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS3d-7	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS3d-7.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS4d-1	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS4d-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS4d-2	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS4d-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS4d-3	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS4d-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS4d-4	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS4d-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS4d-5	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS4d-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS4d-6	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS4d-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	PBS4d-7	Treatment:Control	Model=Negative; RAW_FILE_NAME=PBS4d-7.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt6-1	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt6-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt6-2	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt6-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt6-3	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt6-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt6-4	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt6-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt6-5	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt6-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt6-6	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt6-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt6-7	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt6-7.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt12-1	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt12-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt12-2	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt12-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt12-3	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt12-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt12-4	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt12-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt12-5	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt12-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt12-6	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt12-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt12-7	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt12-7.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt1d-1	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt1d-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt1d-2	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt1d-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt1d-3	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt1d-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt1d-4	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt1d-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt1d-5	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt1d-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt1d-6	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt1d-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt1d-7	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt1d-7.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt2d-1	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt2d-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt2d-2	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt2d-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt2d-3	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt2d-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt2d-4	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt2d-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt2d-5	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt2d-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt2d-6	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt2d-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt2d-7	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt2d-7.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt3d-1	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt3d-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt3d-2	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt3d-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt3d-3	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt3d-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt3d-4	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt3d-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt3d-5	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt3d-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt3d-6	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt3d-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt3d-7	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt3d-7.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt4d-1	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt4d-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt4d-2	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt4d-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt4d-3	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt4d-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt4d-4	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt4d-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt4d-5	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt4d-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt4d-6	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt4d-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	wt4d-7	Treatment:wild-type strain infection group	Model=Negative; RAW_FILE_NAME=wt4d-7.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv6-1	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv6-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv6-2	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv6-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv6-3	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv6-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv6-4	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv6-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv6-5	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv6-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv6-6	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv6-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv6-7	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv6-7.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv12-1	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv12-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv12-2	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv12-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv12-3	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv12-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv12-4	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv12-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv12-5	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv12-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv12-6	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv12-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv12-7	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv12-7.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv1d-1	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv1d-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv1d-2	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv1d-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv1d-3	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv1d-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv1d-4	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv1d-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv1d-5	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv1d-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv1d-6	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv1d-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv1d-7	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv1d-7.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv2d-1	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv2d-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv2d-2	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv2d-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv2d-3	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv2d-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv2d-4	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv2d-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv2d-5	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv2d-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv2d-6	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv2d-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv2d-7	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv2d-7.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv3d-1	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv3d-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv3d-2	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv3d-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv3d-3	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv3d-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv3d-4	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv3d-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv3d-5	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv3d-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv3d-6	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv3d-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv3d-7	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv3d-7.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv4d-1	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv4d-1.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv4d-2	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv4d-2.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv4d-3	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv4d-3.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv4d-4	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv4d-4.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv4d-5	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv4d-5.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv4d-6	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv4d-6.RAW
SUBJECT_SAMPLE_FACTORS           	-	clpv4d-7	Treatment:clpV-RNAi strain infection group	Model=Negative; RAW_FILE_NAME=clpv4d-7.RAW
#COLLECTION
CO:COLLECTION_SUMMARY            	The spleens of E. coioides were collected at 6, 12, 24, 48, 72 and 96 hours post
CO:COLLECTION_SUMMARY            	injection (hpi). After phenotypic observation of spleen tissue, grouper spleen
CO:COLLECTION_SUMMARY            	samples were collected and accurately weighed (~15 mg) and stored in a -80 °C
CO:COLLECTION_SUMMARY            	freezer.
CO:COLLECTION_PROTOCOL_COMMENTS  	E.coioides.pdf
CO:SAMPLE_TYPE                   	Spleen
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	E. coioides of the infection group were intrapleurally injected with 105
TR:TREATMENT_SUMMARY             	colony-forming units per fish (cfu/fish) of P. plecoglossicida (wild-type strain
TR:TREATMENT_SUMMARY             	or clpV-RNAi strain), while control E. coioides received an equivalent volume of
TR:TREATMENT_SUMMARY             	phosphate-buffered saline (PBS).
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	The deep-frozen spleen tissue in each replicate was accurately weighed (about 15
SP:SAMPLEPREP_SUMMARY            	mg) and then transferred to an Eppendorf tube. Each sample was first spiked with
SP:SAMPLEPREP_SUMMARY            	300 μL of methanol containing internal standards and then homogenized at 65 Hz
SP:SAMPLEPREP_SUMMARY            	for 2.5 min. The homogenate was mixed with 1 mL of MTBE, and thoroughly vortexed
SP:SAMPLEPREP_SUMMARY            	for 30 min. Then, 300 μL of Milli-Q water was added to the tube, followed by
SP:SAMPLEPREP_SUMMARY            	vortexing for 1 min and centrifugation for 10 min to form a two-phase system
SP:SAMPLEPREP_SUMMARY            	(12,000 rpm, 4 ℃). Each specimen was prepared by mixing 250 μL of the top
SP:SAMPLEPREP_SUMMARY            	layer plus 150 μL of the bottom layer accurately and then vacuum-dried in a
SP:SAMPLEPREP_SUMMARY            	Speedvac concentrator (Thermo Scientific, USA).
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Waters Acquity
CH:COLUMN_NAME                   	Waters Acquity BEH C8 (100 x 2.1mm, 1.7um)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive HF-X Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	-
MS:MS_RESULTS_FILE               	ST002154_AN003529_Results.txt	UNITS:Peak area	Has m/z:Yes	Has RT:No	RT units:Minutes
#END