#METABOLOMICS WORKBENCH ThomasS_20220601_210455 DATATRACK_ID:3291 STUDY_ID:ST002183 ANALYSIS_ID:AN003576 PROJECT_ID:PR001390
VERSION             	1
CREATED_ON             	June 1, 2022, 9:31 pm
#PROJECT
PR:PROJECT_TITLE                 	Individualized exercise intervention for people with multiple myeloma improves
PR:PROJECT_TITLE                 	quality of life in a randomized controlled trial
PR:PROJECT_TYPE                  	MS untargeted metabolomics analysis
PR:PROJECT_SUMMARY               	Although new treatments have improved survival for multiple myeloma (MM),
PR:PROJECT_SUMMARY               	quality of life remains poor for people with this incurable cancer. We conducted
PR:PROJECT_SUMMARY               	a multi-site randomized, waitlist-controlled trial of an individualized exercise
PR:PROJECT_SUMMARY               	program for people at all stages of MM (n=60). Compared to the waitlist control
PR:PROJECT_SUMMARY               	group, participants of the 12-week intervention had significant improvement in
PR:PROJECT_SUMMARY               	health-related quality of life, mediated through improved MM symptoms,
PR:PROJECT_SUMMARY               	cardiorespiratory fitness and bone pain, with were mostly maintained at
PR:PROJECT_SUMMARY               	follow-up (up to 12 months). Exploratory plasma metabolomics and lipidomics was
PR:PROJECT_SUMMARY               	conducted to delineate molecular mechanisms and biomarkers
PR:INSTITUTE                     	QIMR Berghofer Medical Research Institute
PR:LABORATORY                    	Precision & Systems Biomedicine
PR:LAST_NAME                     	Stoll
PR:FIRST_NAME                    	Thomas
PR:ADDRESS                       	300 Herston Road, Herston, Queensland, 4006, Australia
PR:EMAIL                         	thomas.stoll@qimrberghofer.edu.au
PR:PHONE                         	+61 7 3845 3992
#STUDY
ST:STUDY_TITLE                   	Individualized exercise intervention for people with multiple myeloma improves
ST:STUDY_TITLE                   	quality of life in a randomized controlled trial
ST:STUDY_SUMMARY                 	Although new treatments have improved survival for multiple myeloma (MM),
ST:STUDY_SUMMARY                 	quality of life remains poor for people with this incurable cancer. We conducted
ST:STUDY_SUMMARY                 	a multi-site randomized, waitlist-controlled trial of an individualized exercise
ST:STUDY_SUMMARY                 	program for people at all stages of MM (n=60). Compared to the waitlist control
ST:STUDY_SUMMARY                 	group, participants of the 12-week intervention had significant improvement in
ST:STUDY_SUMMARY                 	health-related quality of life, mediated through improved MM symptoms,
ST:STUDY_SUMMARY                 	cardiorespiratory fitness and bone pain, with were mostly maintained at
ST:STUDY_SUMMARY                 	follow-up (up to 12 months). Exploratory plasma metabolomics and lipidomics was
ST:STUDY_SUMMARY                 	conducted to delineate molecular mechanisms and biomarkers
ST:INSTITUTE                     	QIMR Berghofer Medical Research Institute
ST:LABORATORY                    	Precision & Systems Biomedicine
ST:LAST_NAME                     	Stoll
ST:FIRST_NAME                    	Thomas
ST:ADDRESS                       	300 Herston Road
ST:EMAIL                         	thomas.stoll@qimrberghofer.edu.au
ST:PHONE                         	+61 7 3845 3992
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	MM001_0_p1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM001_0_p1_MetRPpos; RAW_FILE_NAME=MM001_0_p1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM001_1_p1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM001_1_p1_MetRPpos; RAW_FILE_NAME=MM001_1_p1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM001_2_pE1	Group:waitlist | Time_point:2	RAW_FILE_NAME=MM001_2_pE1_MetRPpos; RAW_FILE_NAME=MM001_2_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM001_4_pE1	Group:waitlist | Time_point:4	RAW_FILE_NAME=MM001_4_pE1_MetRPpos; RAW_FILE_NAME=MM001_4_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM002_0_p1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM002_0_p1_MetRPpos; RAW_FILE_NAME=MM002_0_p1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM002_1_p1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM002_1_p1_MetRPpos; RAW_FILE_NAME=MM002_1_p1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM003_0_p1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM003_0_p1_MetRPpos; RAW_FILE_NAME=MM003_0_p1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM003_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM003_1_pE1_MetRPpos; RAW_FILE_NAME=MM003_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM003_3_pE1	Group:exercise | Time_point:3	RAW_FILE_NAME=MM003_3_pE1_MetRPpos; RAW_FILE_NAME=MM003_3_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM004_0_p1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM004_0_p1_MetRPpos; RAW_FILE_NAME=MM004_0_p1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM004_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM004_1_pE1_MetRPpos; RAW_FILE_NAME=MM004_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM004_3_pE1	Group:exercise | Time_point:3	RAW_FILE_NAME=MM004_3_pE1_MetRPpos; RAW_FILE_NAME=MM004_3_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM006_0_p1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM006_0_p1_MetRPpos; RAW_FILE_NAME=MM006_0_p1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM006_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM006_1_pE1_MetRPpos; RAW_FILE_NAME=MM006_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM007_0_p1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM007_0_p1_MetRPpos; RAW_FILE_NAME=MM007_0_p1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM007_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM007_1_pE1_MetRPpos; RAW_FILE_NAME=MM007_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM007_2_pE1	Group:waitlist | Time_point:2	RAW_FILE_NAME=MM007_2_pE1_MetRPpos; RAW_FILE_NAME=MM007_2_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM007_4_pE1	Group:waitlist | Time_point:4	RAW_FILE_NAME=MM007_4_pE1_MetRPpos; RAW_FILE_NAME=MM007_4_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM008_0_p1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM008_0_p1_MetRPpos; RAW_FILE_NAME=MM008_0_p1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM008_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM008_1_pE1_MetRPpos; RAW_FILE_NAME=MM008_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM008_3_pE1	Group:exercise | Time_point:3	RAW_FILE_NAME=MM008_3_pE1_MetRPpos; RAW_FILE_NAME=MM008_3_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM009_0_p1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM009_0_p1_MetRPpos; RAW_FILE_NAME=MM009_0_p1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM009_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM009_1_pE1_MetRPpos; RAW_FILE_NAME=MM009_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM009_2_pE1	Group:waitlist | Time_point:2	RAW_FILE_NAME=MM009_2_pE1_MetRPpos; RAW_FILE_NAME=MM009_2_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM009_4_pE1	Group:waitlist | Time_point:4	RAW_FILE_NAME=MM009_4_pE1_MetRPpos; RAW_FILE_NAME=MM009_4_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM010_0_p1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM010_0_p1_MetRPpos; RAW_FILE_NAME=MM010_0_p1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM010_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM010_1_pE1_MetRPpos; RAW_FILE_NAME=MM010_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM010_3_pE1	Group:exercise | Time_point:3	RAW_FILE_NAME=MM010_3_pE1_MetRPpos; RAW_FILE_NAME=MM010_3_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM011_0_p1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM011_0_p1_MetRPpos; RAW_FILE_NAME=MM011_0_p1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM011_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM011_1_pE1_MetRPpos; RAW_FILE_NAME=MM011_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM011_3_pE1	Group:exercise | Time_point:3	RAW_FILE_NAME=MM011_3_pE1_MetRPpos; RAW_FILE_NAME=MM011_3_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM013_0_p1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM013_0_p1_MetRPpos; RAW_FILE_NAME=MM013_0_p1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM013_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM013_1_pE1_MetRPpos; RAW_FILE_NAME=MM013_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM013_2_pE1	Group:waitlist | Time_point:2	RAW_FILE_NAME=MM013_2_pE1_MetRPpos; RAW_FILE_NAME=MM013_2_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM013_4_pE1	Group:waitlist | Time_point:4	RAW_FILE_NAME=MM013_4_pE1_MetRPpos; RAW_FILE_NAME=MM013_4_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM014_0_p1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM014_0_p1_MetRPpos; RAW_FILE_NAME=MM014_0_p1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM014_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM014_1_pE1_MetRPpos; RAW_FILE_NAME=MM014_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM014_3_pE1	Group:exercise | Time_point:3	RAW_FILE_NAME=MM014_3_pE1_MetRPpos; RAW_FILE_NAME=MM014_3_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM017_0_p2	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM017_0_p2_MetRPpos; RAW_FILE_NAME=MM017_0_p2_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM017_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM017_1_pE1_MetRPpos; RAW_FILE_NAME=MM017_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM017_2_pE1	Group:waitlist | Time_point:2	RAW_FILE_NAME=MM017_2_pE1_MetRPpos; RAW_FILE_NAME=MM017_2_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM017_4_pE1	Group:waitlist | Time_point:4	RAW_FILE_NAME=MM017_4_pE1_MetRPpos; RAW_FILE_NAME=MM017_4_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM018_0_p1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM018_0_p1_MetRPpos; RAW_FILE_NAME=MM018_0_p1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM018_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM018_1_pE1_MetRPpos; RAW_FILE_NAME=MM018_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM019_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM019_0_pE1_MetRPpos; RAW_FILE_NAME=MM019_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM019_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM019_1_pE1_MetRPpos; RAW_FILE_NAME=MM019_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM019_2_pE1	Group:waitlist | Time_point:2	RAW_FILE_NAME=MM019_2_pE1_MetRPpos; RAW_FILE_NAME=MM019_2_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM019_4_pE1	Group:waitlist | Time_point:4	RAW_FILE_NAME=MM019_4_pE1_MetRPpos; RAW_FILE_NAME=MM019_4_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM020_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM020_0_pE1_MetRPpos; RAW_FILE_NAME=MM020_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM020_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM020_1_pE1_MetRPpos; RAW_FILE_NAME=MM020_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM021_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM021_0_pE1_MetRPpos; RAW_FILE_NAME=MM021_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM021_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM021_1_pE1_MetRPpos; RAW_FILE_NAME=MM021_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM021_2_pE1	Group:waitlist | Time_point:2	RAW_FILE_NAME=MM021_2_pE1_MetRPpos; RAW_FILE_NAME=MM021_2_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM022_0_pE1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM022_0_pE1_MetRPpos; RAW_FILE_NAME=MM022_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM022_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM022_1_pE1_MetRPpos; RAW_FILE_NAME=MM022_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM022_3_pE1	Group:exercise | Time_point:3	RAW_FILE_NAME=MM022_3_pE1_MetRPpos; RAW_FILE_NAME=MM022_3_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM023_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM023_0_pE1_MetRPpos; RAW_FILE_NAME=MM023_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM023_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM023_1_pE1_MetRPpos; RAW_FILE_NAME=MM023_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM025_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM025_0_pE1_MetRPpos; RAW_FILE_NAME=MM025_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM025_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM025_1_pE1_MetRPpos; RAW_FILE_NAME=MM025_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM025_2_pE1	Group:waitlist | Time_point:2	RAW_FILE_NAME=MM025_2_pE1_MetRPpos; RAW_FILE_NAME=MM025_2_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM026_0_pE1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM026_0_pE1_MetRPpos; RAW_FILE_NAME=MM026_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM026_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM026_1_pE1_MetRPpos; RAW_FILE_NAME=MM026_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM026_3_pE1	Group:exercise | Time_point:3	RAW_FILE_NAME=MM026_3_pE1_MetRPpos; RAW_FILE_NAME=MM026_3_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM027_0_pE1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM027_0_pE1_MetRPpos; RAW_FILE_NAME=MM027_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM027_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM027_1_pE1_MetRPpos; RAW_FILE_NAME=MM027_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM027_3_pE1	Group:exercise | Time_point:3	RAW_FILE_NAME=MM027_3_pE1_MetRPpos; RAW_FILE_NAME=MM027_3_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM028_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM028_0_pE1_MetRPpos; RAW_FILE_NAME=MM028_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM028_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM028_1_pE1_MetRPpos; RAW_FILE_NAME=MM028_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM028_2_pE1	Group:waitlist | Time_point:2	RAW_FILE_NAME=MM028_2_pE1_MetRPpos; RAW_FILE_NAME=MM028_2_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM028_4_pE1	Group:waitlist | Time_point:4	RAW_FILE_NAME=MM028_4_pE1_MetRPpos; RAW_FILE_NAME=MM028_4_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM030_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM030_0_pE1_MetRPpos; RAW_FILE_NAME=MM030_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM030_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM030_1_pE1_MetRPpos; RAW_FILE_NAME=MM030_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM030_2_pE1	Group:waitlist | Time_point:2	RAW_FILE_NAME=MM030_2_pE1_MetRPpos; RAW_FILE_NAME=MM030_2_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM032_0_pE1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM032_0_pE1_MetRPpos; RAW_FILE_NAME=MM032_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM032_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM032_1_pE1_MetRPpos; RAW_FILE_NAME=MM032_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM033_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM033_0_pE1_MetRPpos; RAW_FILE_NAME=MM033_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM033_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM033_1_pE1_MetRPpos; RAW_FILE_NAME=MM033_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM033_2_pE1	Group:waitlist | Time_point:2	RAW_FILE_NAME=MM033_2_pE1_MetRPpos; RAW_FILE_NAME=MM033_2_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM035_0_pE1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM035_0_pE1_MetRPpos; RAW_FILE_NAME=MM035_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM035_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM035_1_pE1_MetRPpos; RAW_FILE_NAME=MM035_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM035_3_pE1	Group:exercise | Time_point:3	RAW_FILE_NAME=MM035_3_pE1_MetRPpos; RAW_FILE_NAME=MM035_3_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM036_0_pE1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM036_0_pE1_MetRPpos; RAW_FILE_NAME=MM036_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM036_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM036_1_pE1_MetRPpos; RAW_FILE_NAME=MM036_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM036_3_pE1	Group:exercise | Time_point:3	RAW_FILE_NAME=MM036_3_pE1_MetRPpos; RAW_FILE_NAME=MM036_3_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM037_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM037_0_pE1_MetRPpos; RAW_FILE_NAME=MM037_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM037_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM037_1_pE1_MetRPpos; RAW_FILE_NAME=MM037_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM037_2_pE1	Group:waitlist | Time_point:2	RAW_FILE_NAME=MM037_2_pE1_MetRPpos; RAW_FILE_NAME=MM037_2_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM037_4_pE1	Group:waitlist | Time_point:4	RAW_FILE_NAME=MM037_4_pE1_MetRPpos; RAW_FILE_NAME=MM037_4_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM039_0_pE1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM039_0_pE1_MetRPpos; RAW_FILE_NAME=MM039_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM039_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM039_1_pE1_MetRPpos; RAW_FILE_NAME=MM039_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM040_0_pE1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM040_0_pE1_MetRPpos; RAW_FILE_NAME=MM040_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM040_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM040_1_pE1_MetRPpos; RAW_FILE_NAME=MM040_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM041_0_pE1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM041_0_pE1_MetRPpos; RAW_FILE_NAME=MM041_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM041_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM041_1_pE1_MetRPpos; RAW_FILE_NAME=MM041_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM043_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM043_0_pE1_MetRPpos; RAW_FILE_NAME=MM043_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM043_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM043_1_pE1_MetRPpos; RAW_FILE_NAME=MM043_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM043_2_pE1	Group:waitlist | Time_point:2	RAW_FILE_NAME=MM043_2_pE1_MetRPpos; RAW_FILE_NAME=MM043_2_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM044_0_pE1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM044_0_pE1_MetRPpos; RAW_FILE_NAME=MM044_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM044_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM044_1_pE1_MetRPpos; RAW_FILE_NAME=MM044_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM045_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM045_0_pE1_MetRPpos; RAW_FILE_NAME=MM045_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM045_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM045_1_pE1_MetRPpos; RAW_FILE_NAME=MM045_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM046_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM046_0_pE1_MetRPpos; RAW_FILE_NAME=MM046_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM046_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM046_1_pE1_MetRPpos; RAW_FILE_NAME=MM046_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM046_2_pE1	Group:waitlist | Time_point:2	RAW_FILE_NAME=MM046_2_pE1_MetRPpos; RAW_FILE_NAME=MM046_2_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM047_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM047_0_pE1_MetRPpos; RAW_FILE_NAME=MM047_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM047_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM047_1_pE1_MetRPpos; RAW_FILE_NAME=MM047_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM047_2_pE1	Group:waitlist | Time_point:2	RAW_FILE_NAME=MM047_2_pE1_MetRPpos; RAW_FILE_NAME=MM047_2_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM049_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM049_0_pE1_MetRPpos; RAW_FILE_NAME=MM049_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM049_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM049_1_pE1_MetRPpos; RAW_FILE_NAME=MM049_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM050_0_pE1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM050_0_pE1_MetRPpos; RAW_FILE_NAME=MM050_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM050_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM050_1_pE1_MetRPpos; RAW_FILE_NAME=MM050_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM051_0_pE1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM051_0_pE1_MetRPpos; RAW_FILE_NAME=MM051_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM051_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM051_1_pE1_MetRPpos; RAW_FILE_NAME=MM051_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM054_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM054_0_pE1_MetRPpos; RAW_FILE_NAME=MM054_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM054_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM054_1_pE1_MetRPpos; RAW_FILE_NAME=MM054_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM055_0_pE1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM055_0_pE1_MetRPpos; RAW_FILE_NAME=MM055_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM055_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM055_1_pE1_MetRPpos; RAW_FILE_NAME=MM055_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM056_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM056_0_pE1_MetRPpos; RAW_FILE_NAME=MM056_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM056_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM056_1_pE1_MetRPpos; RAW_FILE_NAME=MM056_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM057_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM057_0_pE1_MetRPpos; RAW_FILE_NAME=MM057_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM057_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM057_1_pE1_MetRPpos; RAW_FILE_NAME=MM057_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM058_0_pE1	Group:exercise | Time_point:-	RAW_FILE_NAME=MM058_0_pE1_MetRPpos; RAW_FILE_NAME=MM058_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM058_1_pE1	Group:exercise | Time_point:1	RAW_FILE_NAME=MM058_1_pE1_MetRPpos; RAW_FILE_NAME=MM058_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM059_0_pE1	Group:waitlist | Time_point:-	RAW_FILE_NAME=MM059_0_pE1_MetRPpos; RAW_FILE_NAME=MM059_0_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	MM059_1_pE1	Group:waitlist | Time_point:1	RAW_FILE_NAME=MM059_1_pE1_MetRPpos; RAW_FILE_NAME=MM059_1_pE1_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_01	Group:QC | Time_point:QC	RAW_FILE_NAME=QC_HP_01_MetRPpos; RAW_FILE_NAME=QC_HP_01_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_02	Group:QC | Time_point:QC	RAW_FILE_NAME=QC_HP_02_MetRPpos; RAW_FILE_NAME=QC_HP_02_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_03	Group:QC | Time_point:QC	RAW_FILE_NAME=QC_HP_03_MetRPpos; RAW_FILE_NAME=QC_HP_03_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_04	Group:QC | Time_point:QC	RAW_FILE_NAME=QC_HP_04_MetRPpos; RAW_FILE_NAME=QC_HP_04_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_05	Group:QC | Time_point:QC	RAW_FILE_NAME=QC_HP_05_MetRPpos; RAW_FILE_NAME=QC_HP_05_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_06	Group:QC | Time_point:QC	RAW_FILE_NAME=QC_HP_06_MetRPpos; RAW_FILE_NAME=QC_HP_06_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_07	Group:QC | Time_point:QC	RAW_FILE_NAME=QC_HP_07_MetRPpos; RAW_FILE_NAME=QC_HP_07_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_08	Group:QC | Time_point:QC	RAW_FILE_NAME=QC_HP_08_MetRPpos; RAW_FILE_NAME=QC_HP_08_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_09	Group:QC | Time_point:QC	RAW_FILE_NAME=QC_HP_09_MetRPpos; RAW_FILE_NAME=QC_HP_09_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_10	Group:QC | Time_point:QC	RAW_FILE_NAME=QC_HP_10_MetRPpos; RAW_FILE_NAME=QC_HP_10_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_11	Group:QC | Time_point:QC	RAW_FILE_NAME=QC_HP_11_MetRPpos; RAW_FILE_NAME=QC_HP_11_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_12	Group:QC | Time_point:QC	RAW_FILE_NAME=QC_HP_12_MetRPpos; RAW_FILE_NAME=QC_HP_12_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_13	Group:QC | Time_point:QC	RAW_FILE_NAME=QC_HP_13_MetRPpos; RAW_FILE_NAME=QC_HP_13_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_14	Group:QC | Time_point:QC	RAW_FILE_NAME=QC_HP_14_MetRPpos; RAW_FILE_NAME=QC_HP_14_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_15	Group:QC | Time_point:QC	RAW_FILE_NAME=QC_HP_15_MetRPpos; RAW_FILE_NAME=QC_HP_15_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_16	Group:QC | Time_point:QC	RAW_FILE_NAME=QC_HP_16_MetRPpos; RAW_FILE_NAME=QC_HP_16_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	HP_BlankNegControlExtraction_01	Group:Blank | Time_point:Blank	RAW_FILE_NAME=HP_BlankNegControlExtraction_01_MetRPpos; RAW_FILE_NAME=HP_BlankNegControlExtraction_01_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	HP_BlankNegControlExtraction_02	Group:Blank | Time_point:Blank	RAW_FILE_NAME=HP_BlankNegControlExtraction_02_MetRPpos; RAW_FILE_NAME=HP_BlankNegControlExtraction_02_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	HP_BlankNegControlExtraction_03	Group:Blank | Time_point:Blank	RAW_FILE_NAME=HP_BlankNegControlExtraction_03_MetRPpos; RAW_FILE_NAME=HP_BlankNegControlExtraction_03_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	HP_BlankNegControlExtraction_04	Group:Blank | Time_point:Blank	RAW_FILE_NAME=HP_BlankNegControlExtraction_04_MetRPpos; RAW_FILE_NAME=HP_BlankNegControlExtraction_04_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	HP_BlankNegControlExtraction_05	Group:Blank | Time_point:Blank	RAW_FILE_NAME=HP_BlankNegControlExtraction_05_MetRPpos; RAW_FILE_NAME=HP_BlankNegControlExtraction_05_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	HP_BlankNegControlExtraction_06	Group:Blank | Time_point:Blank	RAW_FILE_NAME=HP_BlankNegControlExtraction_06_MetRPpos; RAW_FILE_NAME=HP_BlankNegControlExtraction_06_MetHILICneg
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE0_it01	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE0_it01; RAW_FILE_NAME=QC_HP_itMS2neg_CE0_it01
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE0_it02	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE0_it02; RAW_FILE_NAME=QC_HP_itMS2neg_CE0_it02
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE0_it03	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE0_it03; RAW_FILE_NAME=QC_HP_itMS2neg_CE0_it03
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE0_it04	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE0_it04; RAW_FILE_NAME=QC_HP_itMS2neg_CE0_it04
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE0_it05	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE0_it05; RAW_FILE_NAME=QC_HP_itMS2neg_CE0_it05
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE0_it06	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE0_it06; RAW_FILE_NAME=QC_HP_itMS2neg_CE0_it06
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE0_it07	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE0_it07; RAW_FILE_NAME=QC_HP_itMS2neg_CE0_it07
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE0_it08	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE0_it08; RAW_FILE_NAME=QC_HP_itMS2neg_CE0_it08
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE10_it01	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE10_it01; RAW_FILE_NAME=QC_HP_itMS2neg_CE10_it01
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE10_it02	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE10_it02; RAW_FILE_NAME=QC_HP_itMS2neg_CE10_it02
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE10_it03	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE10_it03; RAW_FILE_NAME=QC_HP_itMS2neg_CE10_it03
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE10_it04	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE10_it04; RAW_FILE_NAME=QC_HP_itMS2neg_CE10_it04
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE10_it05	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE10_it05; RAW_FILE_NAME=QC_HP_itMS2neg_CE10_it05
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE10_it06	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE10_it06; RAW_FILE_NAME=QC_HP_itMS2neg_CE10_it06
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE10_it07	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE10_it07; RAW_FILE_NAME=QC_HP_itMS2neg_CE10_it07
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE10_it08	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE10_it08; RAW_FILE_NAME=QC_HP_itMS2neg_CE10_it08
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE20_it01	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE20_it01; RAW_FILE_NAME=QC_HP_itMS2neg_CE20_it01
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE20_it02	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE20_it02; RAW_FILE_NAME=QC_HP_itMS2neg_CE20_it02
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE20_it03	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE20_it03; RAW_FILE_NAME=QC_HP_itMS2neg_CE20_it03
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE20_it04	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE20_it04; RAW_FILE_NAME=QC_HP_itMS2neg_CE20_it04
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE20_it05	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE20_it05; RAW_FILE_NAME=QC_HP_itMS2neg_CE20_it05
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE20_it06	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE20_it06; RAW_FILE_NAME=QC_HP_itMS2neg_CE20_it06
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE20_it07	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE20_it07; RAW_FILE_NAME=QC_HP_itMS2neg_CE20_it07
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE20_it08	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE20_it08; RAW_FILE_NAME=QC_HP_itMS2neg_CE20_it08
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE40_it01	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE40_it01; RAW_FILE_NAME=QC_HP_itMS2neg_CE40_it01
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE40_it02	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE40_it02; RAW_FILE_NAME=QC_HP_itMS2neg_CE40_it02
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE40_it03	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE40_it03; RAW_FILE_NAME=QC_HP_itMS2neg_CE40_it03
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE40_it04	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE40_it04; RAW_FILE_NAME=QC_HP_itMS2neg_CE40_it04
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE40_it05	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE40_it05; RAW_FILE_NAME=QC_HP_itMS2neg_CE40_it05
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE40_it06	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE40_it06; RAW_FILE_NAME=QC_HP_itMS2neg_CE40_it06
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE40_it07	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE40_it07; RAW_FILE_NAME=QC_HP_itMS2neg_CE40_it07
SUBJECT_SAMPLE_FACTORS           	-	QC_HP_itMS2_CE40_it08	Group:MS2 | Time_point:MS2	RAW_FILE_NAME=QC_HP_itMS2pos_CE40_it08; RAW_FILE_NAME=QC_HP_itMS2neg_CE40_it08
#COLLECTION
CO:COLLECTION_SUMMARY            	Fasted plasma samples were collected from participants at multiple time points
CO:COLLECTION_SUMMARY            	as depicted in Figure 1, taken from the protocol paper Nicol, J.L.;Woodrow,
CO:COLLECTION_SUMMARY            	C.;Cunningham, B.J.; Mollee, P.;Weber, N.; Smith, M.D.; Nicol, A.J.; Gordon,
CO:COLLECTION_SUMMARY            	L.G.; Hill, M.M.; Skinner, T.L. An Individualized Exercise Intervention for
CO:COLLECTION_SUMMARY            	People with Multiple Myeloma—Study Protocol of a Randomized
CO:COLLECTION_SUMMARY            	Waitlist-Controlled Trial. Curr. Oncol. 2022, 29, 901–923.
CO:COLLECTION_SUMMARY            	https://doi.org/10.3390/curroncol29020077. After overnight fasting, plasma was
CO:COLLECTION_SUMMARY            	collected by an experienced phlebotomist from an antecubital vein using a
CO:COLLECTION_SUMMARY            	23-gauge needle into EDTA-coated blood collection tubes and immediately stored
CO:COLLECTION_SUMMARY            	on ice. Aliquots were prepared in Eppendorf tubes within 4 hours, and frozen at
CO:COLLECTION_SUMMARY            	-80oC until use
CO:SAMPLE_TYPE                   	Blood (plasma)
#TREATMENT
TR:TREATMENT_SUMMARY             	Multiple myeloma patients were randomized to exercise (EX) or waitlist (WT)
TR:TREATMENT_SUMMARY             	groups as detailed in the protocol paper: Nicol, J.L.;Woodrow, C.;Cunningham,
TR:TREATMENT_SUMMARY             	B.J.; Mollee, P.;Weber, N.; Smith, M.D.; Nicol, A.J.; Gordon, L.G.; Hill, M.M.;
TR:TREATMENT_SUMMARY             	Skinner, T.L. An Individualized Exercise Intervention for People with Multiple
TR:TREATMENT_SUMMARY             	Myeloma—Study Protocol of a Randomized Waitlist-Controlled Trial. Curr. Oncol.
TR:TREATMENT_SUMMARY             	2022, 29, 901–923. https://doi.org/10.3390/curroncol29020077. The
TR:TREATMENT_SUMMARY             	individualized exercise program was for 3 months, and follow-up plasma was
TR:TREATMENT_SUMMARY             	collected at 12 months. WT group had usual care for 3 months prior to the same
TR:TREATMENT_SUMMARY             	exercise and follow-up regime
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	In total, 126 human plasma samples from 46 patients were prepared. All samples
SP:SAMPLEPREP_SUMMARY            	(including QCs and blanks) were randomized in one batch and then evenly split
SP:SAMPLEPREP_SUMMARY            	between two 2 mL 96-deepwell plates (Eppendorf #0030 501.306). Human plasma
SP:SAMPLEPREP_SUMMARY            	samples were thawed on ice and briefly vortexed before aliquoting 100 µL plasma
SP:SAMPLEPREP_SUMMARY            	of each sample to a well. In addition, a global sample pool containing equal
SP:SAMPLEPREP_SUMMARY            	volumes (12 µL) of each sample was prepared into a 2 mL Eppendorf tube as
SP:SAMPLEPREP_SUMMARY            	quality control (QC) and 12 x 100 µL aliquots were transferred into wells
SP:SAMPLEPREP_SUMMARY            	across the 2 plates. Finally, blank negative control extraction samples were
SP:SAMPLEPREP_SUMMARY            	prepared by transferring 100 µL of 1X PBS to 6 wells equally across 2 plates.
SP:SAMPLEPREP_SUMMARY            	Ten-times the sample volume of ice-cold butanol/methanol (1:1) containing 50
SP:SAMPLEPREP_SUMMARY            	µg/mL antioxidant 2,6-di-tert-butyl-4-methylphenol (BHT) and 0.5 µg/mL ISTD
SP:SAMPLEPREP_SUMMARY            	4-chloro-L-phenylalanine (PCPA) was added to each well using a multi-channel
SP:SAMPLEPREP_SUMMARY            	pipette. Plates were covered with organic solvent resistant sealing mats
SP:SAMPLEPREP_SUMMARY            	(Eppendorf #0030 127.960) and vortexed for 3 min at 1000 rpm. Samples were then
SP:SAMPLEPREP_SUMMARY            	sonicated for 15 min in an ice-cold water bath sonicator, stored overnight at
SP:SAMPLEPREP_SUMMARY            	-30oC and centrifuged for 30 min at 4,000 x g (4oC). Samples were aliquoted into
SP:SAMPLEPREP_SUMMARY            	96-well V-bottom plates (Greiner #651201) using a liquid handler platform
SP:SAMPLEPREP_SUMMARY            	(AssayMap Bravo, Agilent). From each deepwell plate, 4 x 100 µL and 2 x 200 µL
SP:SAMPLEPREP_SUMMARY            	aliquots were prepared, totalling 8 and 4 plates, respectively. Samples were
SP:SAMPLEPREP_SUMMARY            	dried down (2.5 hrs) using a Genevac EZ-2 vacuum concentrator and fast-stack
SP:SAMPLEPREP_SUMMARY            	swings facilitating drying of 8 plates per batch. Dried sample plates were
SP:SAMPLEPREP_SUMMARY            	covered with AlumaSeal CS sealing film for cold storage (Finneran-Porviar
SP:SAMPLEPREP_SUMMARY            	#FC-100) and stored at -80oC until LC/MS analysis
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	HILIC separation of metabolites was carried out on a Poroshell 120 HILIC-Z
CH:CHROMATOGRAPHY_SUMMARY        	(100Å, 2.7µm, 2.1x100mm, #675775-924, Agilent) column at 30ºC and the MS was
CH:CHROMATOGRAPHY_SUMMARY        	operated in negative ionization mode. Eluent A was 10 mM ammonium acetate and 5
CH:CHROMATOGRAPHY_SUMMARY        	μM medronic acid in ACN/milliQ 90:10 (pH 9) and eluent B was 10 mM ammonium
CH:CHROMATOGRAPHY_SUMMARY        	acetate and 5 μM medronic acid in milliQ (pH 9). Gradient applied was: 0 min
CH:CHROMATOGRAPHY_SUMMARY        	(10% eluent B) – 3.5 min (25% B) – 5.5 min (50% B) – 7.5 min (50% B) –
CH:CHROMATOGRAPHY_SUMMARY        	7.6 min (10% B) – 14 min (10% B). Flow rate was set to 0.25 mL/min with a flow
CH:CHROMATOGRAPHY_SUMMARY        	ramp to 0.5 mL/min during equilibration
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity II
CH:COLUMN_NAME                   	Agilent Poroshell 120 HILIC-Z (2.1x100mm, 2.7 um)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent 6545 QTOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	MS acquisition: Full scan MS data acquisition (m/z 50-1700) was carried out at a
MS:MS_COMMENTS                   	scan rate of 2.5 spectra/sec with the following source conditions applied for
MS:MS_COMMENTS                   	metabolites analysed on reversed-phase: Gas temperature 250°C, gas flow 13
MS:MS_COMMENTS                   	L/min, sheath gas temperature and flow at 400°C and 12 L/min, respectively,
MS:MS_COMMENTS                   	nebulizer 30 psi, fragmentor 135, capillary voltage at +4500 V, nozzle voltage
MS:MS_COMMENTS                   	and CE were zero. For metabolite separation on HILIC conditions were adjusted as
MS:MS_COMMENTS                   	follows: Gas temperature 200°C, gas flow 10 L/min, nebulizer 40 psi, capillary
MS:MS_COMMENTS                   	voltage at -2500 V. MS/MS acquisition: For compound identification, the
MS:MS_COMMENTS                   	‘Iterative MS/MS’ data acquisition mode was employed, i.e. a sample (here:
MS:MS_COMMENTS                   	QC pools) was injected multiple times and precursors previously selected for
MS:MS_COMMENTS                   	MS/MS fragmentation were excluded in subsequent runs. Eight iterative MS/MS
MS:MS_COMMENTS                   	acquisition runs per fixed collision energy (CE) value were performed with CE
MS:MS_COMMENTS                   	values set to 0, 10, 20, and 40 V. Spectral parameters were as follows: MS and
MS:MS_COMMENTS                   	MS/MS mass range was 50-1700; MS and MS/MS acquisition rate was 3 spectra/sec;
MS:MS_COMMENTS                   	quadrupole isolation width was narrow (~1.3 m/z). A maximum of 8 precursors per
MS:MS_COMMENTS                   	cycle were targeted which resulted in a cycle time of 3.1 s. Precursor threshold
MS:MS_COMMENTS                   	was set to 500 counts or 0.001% with an active exclusion of 0.2 min after 1
MS:MS_COMMENTS                   	spectra. Iterative MS/MS settings were enabled with a mass error tolerance of
MS:MS_COMMENTS                   	+/- 5 ppm and retention time exclusion tolerance of +/- 0.1 min. Precursor
MS:MS_COMMENTS                   	charge state was set to 1, 2 and unknown. Precursor abundance-based scan speed
MS:MS_COMMENTS                   	with a target of 25,000 counts/spectrum and the use MS/MS accumulation time
MS:MS_COMMENTS                   	limit were enabled. Precursor purity stringency was kept at 70% and cutoff 0%.
MS:MS_COMMENTS                   	Reference ions were excluded from fragmentation with a delta mass tolerance of
MS:MS_COMMENTS                   	10 ppm. Data processing: Feature extraction was performed separately for each of
MS:MS_COMMENTS                   	the 2 LC/MS analysis modes. A total of 148 data files (126 patient samples, 16
MS:MS_COMMENTS                   	QCs, and 6 blank negative control extractions) were loaded into MassHunter
MS:MS_COMMENTS                   	Profinder (v 10 SP1, Agilent) and assigned to sample groups. Patient samples
MS:MS_COMMENTS                   	were divided into 2 sample groups (waitlist and exercise group), totalling 4
MS:MS_COMMENTS                   	samples groups altogether. First, retention time alignment was conducted using a
MS:MS_COMMENTS                   	QC run as reference file. Spectral feature extraction was then performed using
MS:MS_COMMENTS                   	the recursive feature extraction method employing default settings with minor
MS:MS_COMMENTS                   	adjustments: Peak extraction retention time (Rt) range was restricted to 0.1-5
MS:MS_COMMENTS                   	min and 0.1-7 min for reversed-phase and HILIC, respectively, compound binning
MS:MS_COMMENTS                   	and alignment tolerances were set to 1% + 0.3 min for Rt and 20 ppm + 2 mDa for
MS:MS_COMMENTS                   	mass, integrator Agile 2 was used for peak integration, peak filters were set to
MS:MS_COMMENTS                   	at least 2500 counts and features must have satisfied filter conditions in at
MS:MS_COMMENTS                   	least 75 % of files in at least one sample group. Feature peak area was exported
MS:MS_COMMENTS                   	and data cleaning was performed in accordance with Southam et al. (2021) using
MS:MS_COMMENTS                   	an in-house R script compiled of the following steps. QC samples were removed
MS:MS_COMMENTS                   	from the data matrix area if the total peak area (of all features) exceeded
MS:MS_COMMENTS                   	+/-25% of the median QC total peak area. Features were deleted from the data
MS:MS_COMMENTS                   	matrix if: detected in less than 70% of QC samples; absent across all sample
MS:MS_COMMENTS                   	groups; the mean QC/extract blank area ratio was less than 5; and the peak area
MS:MS_COMMENTS                   	RSD across QC samples was larger than 30%. In addition, duplicate feature
MS:MS_COMMENTS                   	entries were removed.
MS:MS_RESULTS_FILE               	ST002183_AN003576_Results.txt	UNITS:peak area	Has m/z:Neutral masses	Has RT:Yes	RT units:Minutes
#END