#METABOLOMICS WORKBENCH Andre_Gollowitzer_20221012_041732 DATATRACK_ID:3503 STUDY_ID:ST002325 ANALYSIS_ID:AN003794 PROJECT_ID:PR001490
VERSION             	1
CREATED_ON             	October 26, 2022, 6:21 pm
#PROJECT
PR:PROJECT_TITLE                 	Reducing the metabolic burden of rRNA synthesis promotes healthy longevity
PR:PROJECT_SUMMARY               	Ribosome biogenesis is an anabolic process driven by RNA polymerase I (Pol
PR:PROJECT_SUMMARY               	I)-mediated synthesis of pre-ribosomal RNA (pre-rRNA). While Pol I activity was
PR:PROJECT_SUMMARY               	previously linked to longevity, the underlying mechanisms were not studied
PR:PROJECT_SUMMARY               	beyond effects on protein translation and downstream proteostasis. Here we used
PR:PROJECT_SUMMARY               	multi-omics and functional tests to show that curtailment of Pol I activity
PR:PROJECT_SUMMARY               	preserves mitochondrial function and lowers ATP expenditure, thereby promoting
PR:PROJECT_SUMMARY               	longevity in Caenorhabditis elegans. Reduced pre-rRNA synthesis also improved
PR:PROJECT_SUMMARY               	longevity and energy homeostasis in Drosophila melanogaster and human cells,
PR:PROJECT_SUMMARY               	respectively. Conversely, the enhancement of pre-rRNA synthesis boosted growth
PR:PROJECT_SUMMARY               	and neuromuscular performance of young nematodes at the cost of accelerated
PR:PROJECT_SUMMARY               	metabolic decline, mitochondrial stress and premature aging. Moreover,
PR:PROJECT_SUMMARY               	restriction of Pol I activity extended lifespan more potently than translational
PR:PROJECT_SUMMARY               	repression, and retained its geroprotective effects when initiated late in life,
PR:PROJECT_SUMMARY               	showcasing moderation of Pol I activity as an effective longevity treatment not
PR:PROJECT_SUMMARY               	limited by aging.
PR:INSTITUTE                     	University of Innsbruck
PR:DEPARTMENT                    	Michael Popp Institute
PR:LAST_NAME                     	Koeberle
PR:FIRST_NAME                    	Andreas
PR:ADDRESS                       	Mitterweg 24, Innsbruck, Tyrol, 6020, Austria
PR:EMAIL                         	andreas.koeberle@uibk.ac.at
PR:PHONE                         	+43 512 507 57903
PR:FUNDING_SOURCE                	Leibniz Association, Thüringer Aufbaubank (2019 FGR 0082), Free State of
PR:FUNDING_SOURCE                	Thuringia (RegenerAging—FSU-I-03/14), Carl-Zeiss-Stiftung (IMPULS),
PR:FUNDING_SOURCE                	Phospholipid Research Center (AKO-2019-070/2-1)
PR:CONTRIBUTORS                  	Samim Sharifi, Finja Witt, André Gollowitzer, Oliver Werz, Holger Bierhoff,
PR:CONTRIBUTORS                  	Maria Ermolaeva
#STUDY
ST:STUDY_TITLE                   	Determination of changes in free fatty acid levels in C. elegans depleted of
ST:STUDY_TITLE                   	tif-IA and ncl-1
ST:STUDY_SUMMARY                 	Analysis of free fatty acids in young (d2), middle age (d6) and old age (d12) C.
ST:STUDY_SUMMARY                 	elegans with tif-IA or ncl-1 knockdown.
ST:INSTITUTE                     	University of Innsbruck
ST:DEPARTMENT                    	Michael Popp Institute
ST:LAST_NAME                     	Koeberle
ST:FIRST_NAME                    	Andreas
ST:ADDRESS                       	Mitterweg 24, Innsbruck, Tyrol, 6020, Austria
ST:EMAIL                         	andreas.koeberle@uibk.ac.at
ST:PHONE                         	+43 512 507 57903
#SUBJECT
SU:SUBJECT_TYPE                  	Invertebrate
SU:SUBJECT_SPECIES               	Caenorhabditis elegans
SU:TAXONOMY_ID                   	6239
SU:AGE_OR_AGE_RANGE              	Young, middle aged and old
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_1	Treatment:ctrl RNAi | Days of adulthood:2	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_2	Treatment:ctrl RNAi | Days of adulthood:6	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_3	Treatment:ctrl RNAi | Days of adulthood:12	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_4	Treatment:tif-IA RNAi | Days of adulthood:2	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_5	Treatment:tif-IA RNAi | Days of adulthood:6	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_6	Treatment:tif-IA RNAi | Days of adulthood:12	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_7	Treatment:ncl-1 RNAi | Days of adulthood:2	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_8	Treatment:ncl-1 RNAi | Days of adulthood:6	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_9	Treatment:ncl-1 RNAi | Days of adulthood:12	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_10	Treatment:ctrl RNAi | Days of adulthood:2	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_11	Treatment:ctrl RNAi | Days of adulthood:6	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_12	Treatment:ctrl RNAi | Days of adulthood:12	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_13	Treatment:tif-IA RNAi | Days of adulthood:2	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_14	Treatment:tif-IA RNAi | Days of adulthood:6	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_15	Treatment:tif-IA RNAi | Days of adulthood:12	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_16	Treatment:ncl-1 RNAi | Days of adulthood:2	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_17	Treatment:ncl-1 RNAi | Days of adulthood:6	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_18	Treatment:ncl-1 RNAi | Days of adulthood:12	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_19	Treatment:ctrl RNAi | Days of adulthood:2	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_20	Treatment:ctrl RNAi | Days of adulthood:6	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_21	Treatment:ctrl RNAi | Days of adulthood:12	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_22	Treatment:tif-IA RNAi | Days of adulthood:2	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_23	Treatment:tif-IA RNAi | Days of adulthood:6	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_24	Treatment:tif-IA RNAi | Days of adulthood:12	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_25	Treatment:ncl-1 RNAi | Days of adulthood:2	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_26	Treatment:ncl-1 RNAi | Days of adulthood:6	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_27	Treatment:ncl-1 RNAi | Days of adulthood:12	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_28	Treatment:ctrl RNAi | Days of adulthood:2	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_29	Treatment:ctrl RNAi | Days of adulthood:6	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_30	Treatment:ctrl RNAi | Days of adulthood:12	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_31	Treatment:tif-IA RNAi | Days of adulthood:2	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_32	Treatment:tif-IA RNAi | Days of adulthood:6	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_33	Treatment:tif-IA RNAi | Days of adulthood:12	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_34	Treatment:ncl-1 RNAi | Days of adulthood:2	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_35	Treatment:ncl-1 RNAi | Days of adulthood:6	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_36	Treatment:ncl-1 RNAi | Days of adulthood:12	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_37	Treatment:ctrl RNAi | Days of adulthood:2	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_38	Treatment:ctrl RNAi | Days of adulthood:6	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_39	Treatment:ctrl RNAi | Days of adulthood:12	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_40	Treatment:tif-IA RNAi | Days of adulthood:2	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_41	Treatment:tif-IA RNAi | Days of adulthood:6	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_42	Treatment:tif-IA RNAi | Days of adulthood:12	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_43	Treatment:ncl-1 RNAi | Days of adulthood:2	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_44	Treatment:ncl-1 RNAi | Days of adulthood:6	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
SUBJECT_SAMPLE_FACTORS           	-	200301_Ermoleva_FFA_45	Treatment:ncl-1 RNAi | Days of adulthood:12	RAW_FILE_NAME=200301_Ermoleva_FFA_FW.wiff
#COLLECTION
CO:COLLECTION_SUMMARY            	Worms were washed and pelleted, M9 media was removed with ~100µl remaining in
CO:COLLECTION_SUMMARY            	the sample, and the sample was snap frozen.
CO:SAMPLE_TYPE                   	Worms
#TREATMENT
TR:TREATMENT_SUMMARY             	Ncl-1 and tif-IA knockdown was achieved by feeding the worms for 2 generations
TR:TREATMENT_SUMMARY             	with bacteria (from the Ahringer RNAi feeding library, Source BioScience Ltd.)
TR:TREATMENT_SUMMARY             	producing the corresponding dsRNA. Worms were cultured under standard conditions
TR:TREATMENT_SUMMARY             	as described in ‘Wormbook’, F1 was grown at 15°C and F2 at 20°C, and F2
TR:TREATMENT_SUMMARY             	animals were grown on RNAi until days 2, 6 or 12 of adulthood.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Free fatty acids were extracted from C. elegans by successive addition of PBS pH
SP:SAMPLEPREP_SUMMARY            	7.4, methanol, chloroform, and saline to a final ratio of 14:34:35:17.
SP:SAMPLEPREP_SUMMARY            	Evaporation of the organic layer yielded a lipid film that was dissolved in
SP:SAMPLEPREP_SUMMARY            	methanol and subjected to UPLC-MS/MS.
SP:EXTRACT_STORAGE               	-20℃
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Chromatographic separation of free fatty acids was carried out on an Acquity BEH
CH:CHROMATOGRAPHY_SUMMARY        	C8 column (1.7 μm, 2.1×100 mm, Waters, Milford, MA) using an Acquity UHPLC.
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Waters Acquity H-Class
CH:COLUMN_NAME                   	Waters Acquity BEH C8 (100 x 2.1mm, 1.7um)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	ABI Sciex 5500 QTrap
MS:INSTRUMENT_TYPE               	QTRAP
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	Multiple ion monitoring with pre-optimized settings and subsequent automated
MS:MS_COMMENTS                   	integration of selected signals using Analyst 1.6.2 or Analyst 1.6.3 (Sciex).
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	relative units
MS_METABOLITE_DATA_START
Samples	200301_Ermoleva_FFA_1	200301_Ermoleva_FFA_2	200301_Ermoleva_FFA_3	200301_Ermoleva_FFA_4	200301_Ermoleva_FFA_5	200301_Ermoleva_FFA_6	200301_Ermoleva_FFA_7	200301_Ermoleva_FFA_8	200301_Ermoleva_FFA_9	200301_Ermoleva_FFA_10	200301_Ermoleva_FFA_11	200301_Ermoleva_FFA_12	200301_Ermoleva_FFA_13	200301_Ermoleva_FFA_14	200301_Ermoleva_FFA_15	200301_Ermoleva_FFA_16	200301_Ermoleva_FFA_17	200301_Ermoleva_FFA_18	200301_Ermoleva_FFA_19	200301_Ermoleva_FFA_20	200301_Ermoleva_FFA_21	200301_Ermoleva_FFA_22	200301_Ermoleva_FFA_23	200301_Ermoleva_FFA_24	200301_Ermoleva_FFA_25	200301_Ermoleva_FFA_26	200301_Ermoleva_FFA_27	200301_Ermoleva_FFA_28	200301_Ermoleva_FFA_29	200301_Ermoleva_FFA_30	200301_Ermoleva_FFA_31	200301_Ermoleva_FFA_32	200301_Ermoleva_FFA_33	200301_Ermoleva_FFA_34	200301_Ermoleva_FFA_35	200301_Ermoleva_FFA_36	200301_Ermoleva_FFA_37	200301_Ermoleva_FFA_38	200301_Ermoleva_FFA_39	200301_Ermoleva_FFA_40	200301_Ermoleva_FFA_41	200301_Ermoleva_FFA_42	200301_Ermoleva_FFA_43	200301_Ermoleva_FFA_44	200301_Ermoleva_FFA_45
Factors	Treatment:ctrl RNAi | Days of adulthood:2	Treatment:ctrl RNAi | Days of adulthood:6	Treatment:ctrl RNAi | Days of adulthood:12	Treatment:tif-IA RNAi | Days of adulthood:2	Treatment:tif-IA RNAi | Days of adulthood:6	Treatment:tif-IA RNAi | Days of adulthood:12	Treatment:ncl-1 RNAi | Days of adulthood:2	Treatment:ncl-1 RNAi | Days of adulthood:6	Treatment:ncl-1 RNAi | Days of adulthood:12	Treatment:ctrl RNAi | Days of adulthood:2	Treatment:ctrl RNAi | Days of adulthood:6	Treatment:ctrl RNAi | Days of adulthood:12	Treatment:tif-IA RNAi | Days of adulthood:2	Treatment:tif-IA RNAi | Days of adulthood:6	Treatment:tif-IA RNAi | Days of adulthood:12	Treatment:ncl-1 RNAi | Days of adulthood:2	Treatment:ncl-1 RNAi | Days of adulthood:6	Treatment:ncl-1 RNAi | Days of adulthood:12	Treatment:ctrl RNAi | Days of adulthood:2	Treatment:ctrl RNAi | Days of adulthood:6	Treatment:ctrl RNAi | Days of adulthood:12	Treatment:tif-IA RNAi | Days of adulthood:2	Treatment:tif-IA RNAi | Days of adulthood:6	Treatment:tif-IA RNAi | Days of adulthood:12	Treatment:ncl-1 RNAi | Days of adulthood:2	Treatment:ncl-1 RNAi | Days of adulthood:6	Treatment:ncl-1 RNAi | Days of adulthood:12	Treatment:ctrl RNAi | Days of adulthood:2	Treatment:ctrl RNAi | Days of adulthood:6	Treatment:ctrl RNAi | Days of adulthood:12	Treatment:tif-IA RNAi | Days of adulthood:2	Treatment:tif-IA RNAi | Days of adulthood:6	Treatment:tif-IA RNAi | Days of adulthood:12	Treatment:ncl-1 RNAi | Days of adulthood:2	Treatment:ncl-1 RNAi | Days of adulthood:6	Treatment:ncl-1 RNAi | Days of adulthood:12	Treatment:ctrl RNAi | Days of adulthood:2	Treatment:ctrl RNAi | Days of adulthood:6	Treatment:ctrl RNAi | Days of adulthood:12	Treatment:tif-IA RNAi | Days of adulthood:2	Treatment:tif-IA RNAi | Days of adulthood:6	Treatment:tif-IA RNAi | Days of adulthood:12	Treatment:ncl-1 RNAi | Days of adulthood:2	Treatment:ncl-1 RNAi | Days of adulthood:6	Treatment:ncl-1 RNAi | Days of adulthood:12
Myristic acid	45.541074827939700	39.436878066675800	19.695682397466600	35.956095714706300	14.848095849379500	29.301745635910200	16.766509517564100	14.562071050104900	15.737587381251100	20.906563742045200	31.397991222440000	28.188746917731500	41.658764776534500	36.906906020254500	16.031009235775500	21.683793721127600	18.309214464279400	29.725609756097600	46.428980855210400	41.846906150084600	10.825267447784000	55.558387277639000	5.652911249293390	24.171981073853100	36.379986919555300	37.929222144358800	35.610320213741700	46.681550639334300	22.157434402332400	36.130068245684500	44.181326116373500	14.093959731543600	22.836538461538500	22.903094462540700	2.493765586034910	37.847688086989600	54.311435161074300	30.355021776428700	14.966699094514700	29.537734455767200	35.410216718266300	36.699507389162600	35.990261458664100	34.735260983852800	34.868754631615900
Hexadecenoic acid	11.055791477522300	9.148438574769570	18.807445740325900	11.060179149669900	8.600770218228500	16.608478802992500	6.912197369820280	8.054478859215530	3.082989783115250	6.892273761113790	2.151022603084290	12.216991705895500	13.907326632530500	13.345537216924000	17.586256400440300	2.231230948116020	0.000000000000000	12.576219512195100	12.438381290840300	11.190520970472100	8.182628629648500	8.767011570416430	15.381869030972000	13.114585476239500	11.118378024852800	5.737561317449190	9.730031502970850	7.458899939111020	12.303206997084500	7.455410907839650	10.148849797023000	0.000000000000000	13.942307692307700	4.275244299674270	9.289276807980050	7.149007749764700	12.499070009671900	13.461792266068400	6.735014592531620	6.719834588687050	8.191434468524250	26.108374384236500	3.387318725521330	5.867442733758920	4.524357424236510
Octadecenoic acid	14.643432420559400	19.489154858531700	20.468062099328000	14.718869590815400	25.531307944658400	35.162094763092300	15.951224699585300	15.468866683261600	21.867718229073300	21.595791118156500	23.244921678491500	26.619592019726500	19.912763132941400	15.648528152587900	37.086663157391000	2.828320920147070	12.323509735572700	16.768292682926800	17.195918835263100	0.000000000000000	24.834437086092700	0.000000000000000	54.743982624735900	20.057601316601500	0.000000000000000	0.000000000000000	14.355784184711100	18.012989648873600	17.201166180758000	18.925273842977600	0.000000000000000	0.000000000000000	0.000000000000000	0.000000000000000	0.000000000000000	20.425736427899200	20.459787218212900	17.817077999208100	14.966699094514700	23.113277211637900	27.089783281733700	11.494252873563200	8.997565364666030	6.102140443109270	30.422403369866200
Octadecadienoic acid	0.000000000000000	0.068785252441877	0.000000000000000	0.000000000000000	5.348737697903300	1.271820448877810	2.552195951933640	7.947797020020620	1.756587202007530	0.000000000000000	4.128575641403720	0.812598072181125	0.000000000000000	1.033393368567130	1.004928937168010	0.754218912039219	0.070420055631844	0.647865853658537	0.000000000000000	0.000000000000000	2.738155883851250	0.000000000000000	2.975216446996520	0.000000000000000	0.000000000000000	0.000000000000000	2.631893767197030	0.000000000000000	2.215743440233240	0.200722601364914	0.000000000000000	0.000000000000000	0.000000000000000	0.000000000000000	0.000000000000000	7.269159140517050	0.000000000000000	0.000000000000000	0.000000000000000	0.000000000000000	0.000000000000000	0.000000000000000	0.000000000000000	0.093879083740143	0.000000000000000
Eicosatetraenoic acid	2.086689119929710	3.402577154124820	6.503437089673280	5.000210269565580	6.931964056482670	3.802992518703240	5.161107369465810	6.920095302443010	10.055565513532900	3.668987065499580	3.464187208354290	4.898004931629680	3.723370630254760	5.311641914435030	6.350193807723600	7.891015367210330	5.531495369881340	6.402439024390240	2.097902097902100	4.527929283430510	9.389327559857360	5.092002650491870	3.674392312040700	9.535075087430570	4.120340091563110	5.089348283111420	6.208876659887550	1.585650497260000	4.373177842565600	6.397316052073180	7.171853856562920	18.389261744966400	11.237980769230800	5.690146579804560	10.642144638404000	5.020325610268940	1.421025221337700	1.940081826580440	10.416822569782200	7.413971348397580	5.572755417956660	6.568144499178980	3.673123742987190	5.510702215546380	4.169429384921410
Eicosapentaenoic acid	26.673012154048900	28.454166093456200	34.525372673206100	33.264645275242900	38.739124233347600	13.852867830423900	52.656765091630900	47.046691084954300	47.499551891019900	46.936384313184900	35.613301646226200	27.264066352835700	20.797774827738800	27.753993327231400	21.940948461501700	64.611420131359800	63.765360374634700	33.879573170731700	21.838816920784100	42.434643596012800	44.030183392766200	30.582598501452700	17.571628335961400	33.120757045875300	48.381294964028800	51.243868255080600	31.463093671491800	26.260909275421100	41.749271137026200	30.891208350060200	38.497970230040600	67.516778523489900	51.983173076923100	67.131514657980500	77.574812967581100	22.288082984560500	11.308682389703100	36.426026131714400	52.914764648656700	33.215182395510300	23.735810113519100	19.129720853858800	47.951730708161300	47.690574539992500	26.015055189360000
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	Standardized name	Alternative name	Formula	Exact mass	Super class	Main class	Sub class
Myristic acid	Myristic acid	FA 14:0	C14H28O2	228.2089	Fatty Acyls	Fatty acids	Saturated FA
Hexadecenoic acid	Hexadecenoic acid	FA 16:1	C16H30O2	254.2246	Fatty Acyls	Fatty acids	Unsaturated FA
Octadecenoic acid	Octadecenoic acid	FA 18:1	C18H34O2	282.2559	Fatty Acyls	Fatty acids	Unsaturated FA
Octadecadienoic acid	Octadecadienoic acid	FA 18:2	C18H32O2	280.2402	Fatty Acyls	Fatty acids	Unsaturated FA
Eicosatetraenoic acid	Arachidonic acid	FA 20:4	C20H32O2	304.2402	Fatty Acyls	Fatty acids	Unsaturated FA
Eicosapentaenoic acid	Eicosapentaenoic acid	FA 20:5	C20H30O2	302.2246	Fatty Acyls	Fatty acids	Unsaturated FA
METABOLITES_END
#END