#METABOLOMICS WORKBENCH Chen_xy2022_20221008_224006 DATATRACK_ID:3498 STUDY_ID:ST002329 ANALYSIS_ID:AN003799 VERSION 1 CREATED_ON 10-28-2022 #PROJECT PR:PROJECT_TITLE Lipidomic profiling indicate protection of hypoxic-ischemic brain injury on PR:PROJECT_TITLE neonatal rats ingesting Acer truncatum Bunge oil PR:PROJECT_SUMMARY Hypoxic ischemic encephalopathy (HIE) has a great impact on the growth and PR:PROJECT_SUMMARY development of newborns. At present, there is a lack of other effective PR:PROJECT_SUMMARY intervention measures except hypothermia therapy, and the effective rate of PR:PROJECT_SUMMARY hypothermia therapy is less than 50%. Using high-throughput metabonomics, we PR:PROJECT_SUMMARY analyzed the serum and brain tissues of 7-day-old HIE mice, mice fed with Acer PR:PROJECT_SUMMARY truncatum Bunge seed oil (ASO) for 30 days and mice fed with their respective PR:PROJECT_SUMMARY ages. All the results revealed that a variety of metabolites changed PR:PROJECT_SUMMARY significantly during injury and repair, and there were many significant PR:PROJECT_SUMMARY enrichment pathways of designed lipid metabolism and neurotrophic factors. PR:PROJECT_SUMMARY Multi-group comparative analysis identified metabolites that may be related to PR:PROJECT_SUMMARY the injury and repair of hypoxic-ischemic encephalopathy, such as glycerol PR:PROJECT_SUMMARY phospholipids and sphingomyelins. Our results suggest that ASO may be a PR:PROJECT_SUMMARY potential special medical food for ischemic hypoxia newborns. PR:INSTITUTE Bao Feng Key Laboratory of Genetics and Metabolism PR:LAST_NAME Chen PR:FIRST_NAME xianyang PR:ADDRESS Room 525, Floor 5, Building 3, Yard 10, Anxiang Street, Airport Economic Core PR:ADDRESS Zone, Shunyi District, Beijing PR:EMAIL cxyibcas@163.com PR:PHONE 13681398052 PR:DOI http://dx.doi.org/10.21228/M8R403 #STUDY ST:STUDY_TITLE Serum metabonomics of rats in sham, HIE, CO and ASO groups were compared. ST:STUDY_SUMMARY To study the cerebral protective of nervonic acid after hypoxia-ischemia (HI), ST:STUDY_SUMMARY rats were randomly divided into two groups: Sham group (n=12), HIE group (n=8). ST:STUDY_SUMMARY After 48 hours of successful modeling, we re-group the HIE group: CO group (n=5) ST:STUDY_SUMMARY and ASO group (n=8). The ASO group were treated by Acer truncatum Bunge seed oil ST:STUDY_SUMMARY for 30 days, rats in the CO group were treated by an equal volume of corn oil. ST:INSTITUTE Bao Feng Key Laboratory of Genetics and Metabolism ST:LAST_NAME Chen ST:FIRST_NAME xianyang ST:ADDRESS Room 525, Floor 5, Building 3, Yard 10, Anxiang Street, Airport Economic Core ST:ADDRESS Zone, Shunyi District, Beijing ST:EMAIL cxyibcas@163.com ST:PHONE 13681398052 ST:SUBMIT_DATE 2022-10-08 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Rattus norvegicus SU:TAXONOMY_ID 10116 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - 20210624_91 Group:ASO1 Pos or neg=pos; RAW_FILE_NAME=20210624_91.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_91 Group:ASO1 Pos or neg=neg; RAW_FILE_NAME=20210626_91.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_29 Group:ASO2 Pos or neg=pos; RAW_FILE_NAME=20210624_29.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_29 Group:ASO2 Pos or neg=neg; RAW_FILE_NAME=20210626_29.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_56 Group:ASO3 Pos or neg=pos; RAW_FILE_NAME=20210624_56.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_56 Group:ASO3 Pos or neg=neg; RAW_FILE_NAME=20210626_56.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_13 Group:ASO4 Pos or neg=pos; RAW_FILE_NAME=20210624_13.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_13 Group:ASO4 Pos or neg=neg; RAW_FILE_NAME=20210626_13.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_31 Group:ASO5 Pos or neg=pos; RAW_FILE_NAME=20210624_31.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_31 Group:ASO5 Pos or neg=neg; RAW_FILE_NAME=20210626_31.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_44 Group:ASO6 Pos or neg=pos; RAW_FILE_NAME=20210624_44.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_44 Group:ASO6 Pos or neg=neg; RAW_FILE_NAME=20210626_44.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_52 Group:ASO7 Pos or neg=pos; RAW_FILE_NAME=20210624_52.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_52 Group:ASO7 Pos or neg=neg; RAW_FILE_NAME=20210626_52.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_61 Group:ASO8 Pos or neg=pos; RAW_FILE_NAME=20210624_61.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_61 Group:ASO8 Pos or neg=neg; RAW_FILE_NAME=20210626_61.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_41 Group:CO1 Pos or neg=pos; RAW_FILE_NAME=20210624_41.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_41 Group:CO1 Pos or neg=neg; RAW_FILE_NAME=20210626_41.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_49 Group:CO2 Pos or neg=pos; RAW_FILE_NAME=20210624_49.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_49 Group:CO2 Pos or neg=neg; RAW_FILE_NAME=20210626_49.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_36 Group:CO3 Pos or neg=pos; RAW_FILE_NAME=20210624_36.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_36 Group:CO3 Pos or neg=neg; RAW_FILE_NAME=20210626_36.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_24 Group:CO4 Pos or neg=pos; RAW_FILE_NAME=20210624_24.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_24 Group:CO4 Pos or neg=neg; RAW_FILE_NAME=20210626_24.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_68 Group:CO5 Pos or neg=pos; RAW_FILE_NAME=20210624_68.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_68 Group:CO5 Pos or neg=neg; RAW_FILE_NAME=20210626_68.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_12 Group:HIE1 Pos or neg=pos; RAW_FILE_NAME=20210624_12.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_12 Group:HIE1 Pos or neg=neg; RAW_FILE_NAME=20210626_12.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_23 Group:HIE2 Pos or neg=pos; RAW_FILE_NAME=20210624_23.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_23 Group:HIE2 Pos or neg=neg; RAW_FILE_NAME=20210626_23.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_35 Group:HIE3 Pos or neg=pos; RAW_FILE_NAME=20210624_35.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_35 Group:HIE3 Pos or neg=neg; RAW_FILE_NAME=20210626_35.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_47 Group:HIE4 Pos or neg=pos; RAW_FILE_NAME=20210624_47.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_47 Group:HIE4 Pos or neg=neg; RAW_FILE_NAME=20210626_47.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_59 Group:HIE5 Pos or neg=pos; RAW_FILE_NAME=20210624_59.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_59 Group:HIE5 Pos or neg=neg; RAW_FILE_NAME=20210626_59.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_79 Group:HIE6 Pos or neg=pos; RAW_FILE_NAME=20210624_79.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_79 Group:HIE6 Pos or neg=neg; RAW_FILE_NAME=20210626_79.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_63 Group:HIE7 Pos or neg=pos; RAW_FILE_NAME=20210624_63.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_63 Group:HIE7 Pos or neg=neg; RAW_FILE_NAME=20210626_63.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_75 Group:HIE8 Pos or neg=pos; RAW_FILE_NAME=20210624_75.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_75 Group:HIE8 Pos or neg=neg; RAW_FILE_NAME=20210626_75.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_64 Group:sham1 Pos or neg=pos; RAW_FILE_NAME=20210624_64.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_64 Group:sham1 Pos or neg=neg; RAW_FILE_NAME=20210626_64.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_45 Group:sham10 Pos or neg=pos; RAW_FILE_NAME=20210624_45.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_45 Group:sham10 Pos or neg=neg; RAW_FILE_NAME=20210626_45.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_43 Group:sham11 Pos or neg=pos; RAW_FILE_NAME=20210624_43.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_43 Group:sham11 Pos or neg=neg; RAW_FILE_NAME=20210626_43.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_55 Group:sham12 Pos or neg=pos; RAW_FILE_NAME=20210624_55.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_55 Group:sham12 Pos or neg=neg; RAW_FILE_NAME=20210626_55.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_32 Group:sham2 Pos or neg=pos; RAW_FILE_NAME=20210624_32.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_32 Group:sham2 Pos or neg=neg; RAW_FILE_NAME=20210626_32.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_18 Group:sham3 Pos or neg=pos; RAW_FILE_NAME=20210624_18.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_18 Group:sham3 Pos or neg=neg; RAW_FILE_NAME=20210626_18.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_26 Group:sham4 Pos or neg=pos; RAW_FILE_NAME=20210624_26.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_26 Group:sham4 Pos or neg=neg; RAW_FILE_NAME=20210626_26.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_78 Group:sham5 Pos or neg=pos; RAW_FILE_NAME=20210624_78.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_78 Group:sham5 Pos or neg=neg; RAW_FILE_NAME=20210626_78.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_80 Group:sham6 Pos or neg=pos; RAW_FILE_NAME=20210624_80.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_80 Group:sham6 Pos or neg=neg; RAW_FILE_NAME=20210626_80.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_15 Group:sham7 Pos or neg=pos; RAW_FILE_NAME=20210624_15.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_15 Group:sham7 Pos or neg=neg; RAW_FILE_NAME=20210626_15.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_60 Group:sham8 Pos or neg=pos; RAW_FILE_NAME=20210624_60.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_60 Group:sham8 Pos or neg=neg; RAW_FILE_NAME=20210626_60.mzXML SUBJECT_SAMPLE_FACTORS - 20210624_28 Group:sham9 Pos or neg=pos; RAW_FILE_NAME=20210624_28.mzXML SUBJECT_SAMPLE_FACTORS - 20210626_28 Group:sham9 Pos or neg=neg; RAW_FILE_NAME=20210626_28.mzXML #COLLECTION CO:COLLECTION_SUMMARY After sevoflurane inhalation anesthesia, use a blood collection vessel without CO:COLLECTION_SUMMARY any additives (without any anticoagulant or accelerator) to collect whole blood. CO:COLLECTION_SUMMARY Let it stand at 4 ℃ for about 1h. After the blood coagulates, centrifuge at CO:COLLECTION_SUMMARY low speed (3000 rpm, 10 min, 4 ℃), take the upper serum, and centrifuge at CO:COLLECTION_SUMMARY high speed (12000 rpm, 10 min, 4 ℃). After centrifugation, take the upper CO:COLLECTION_SUMMARY serum and transfer it to a 1.5mL centrifuge tube, 200 μ L Carry out equal CO:COLLECTION_SUMMARY subpackaging, make marks, seal with sealing film, and freeze at - 80 ℃. CO:SAMPLE_TYPE Blood (serum) #TREATMENT TR:TREATMENT_SUMMARY To study the cerebral protective of nervonic acid after hypoxia-ischemia (HI), TR:TREATMENT_SUMMARY rats were randomly divided into two groups: Sham group (n=12), HIE group (n=8). TR:TREATMENT_SUMMARY After 48 hours of successful modeling, we re-group the HIE group: CO group (n=5) TR:TREATMENT_SUMMARY and ASO group (n=8). The ASO group were treated by Acer truncatum Bunge seed oil TR:TREATMENT_SUMMARY for 30 days, rats in the CO group were treated by an equal volume of corn oil25. TR:TREATMENT_SUMMARY Based on the individual recommended intake NA of 0.3 g/kg/d, the equivalent dose TR:TREATMENT_SUMMARY of the drug was calculated according to the bodyweight of the human and the TR:TREATMENT_SUMMARY animal, in which the daily dose of the rat was equivalent to six times that of TR:TREATMENT_SUMMARY adults. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY We use isopropanol (IPA) to extract lipids from serum and brain tissue. In SP:SAMPLEPREP_SUMMARY brief, 200 μL of serum was extracted with 600 μL of precooled IPA temperature SP:SAMPLEPREP_SUMMARY of 4°C, vortexed for 1 minute and incubated for 10 minutes at room temperature; SP:SAMPLEPREP_SUMMARY the extracted mixture was stored overnight at -20 ° C. Samples were centrifuged SP:SAMPLEPREP_SUMMARY for 20 minutes at 14000 rpm, and the supernatants were transferred into a new SP:SAMPLEPREP_SUMMARY centrifuge tube and diluted to 1:10 with IPA/ACN/H2O (2.5:1:1, v: v: v). Before SP:SAMPLEPREP_SUMMARY LC/MS analysis, the sample was stored at -80°C. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY ACQUITYUPLC I-CLASS (Waters) and XEVO-G2XS quadrupole time-of-flight (QTOF) mass CH:CHROMATOGRAPHY_SUMMARY spectrometry (Waters) with ESI were used to analyze the samples. CH:METHODS_FILENAME Chrom__methods_.pdf CH:INSTRUMENT_NAME Waters Acquity I-Class CH:COLUMN_NAME Waters Acquity BEH C18 (100 x 2mm, 1.7um) CH:CHROMATOGRAPHY_TYPE UPLC #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Waters Xevo-G2-XS MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:MS_COMMENTS - MS:ION_MODE POSITIVE MS:MS_RESULTS_FILE ST002329_AN003799_Results.txt UNITS:Peak area Has m/z:Yes Has RT:Yes RT units:Minutes #END