#METABOLOMICS WORKBENCH Chen_xy2022_20221020_185700 DATATRACK_ID:3529 STUDY_ID:ST002333 ANALYSIS_ID:AN003812 PROJECT_ID:PR001493 VERSION 1 CREATED_ON November 2, 2022, 4:56 pm #PROJECT PR:PROJECT_TITLE Lipidomic profiling indicate protection of hypoxic-ischemic brain injury on PR:PROJECT_TITLE neonatal rats ingesting Acer truncatum Bunge oil PR:PROJECT_SUMMARY Hypoxic ischemic encephalopathy (HIE) has a great impact on the growth and PR:PROJECT_SUMMARY development of newborns. At present, there is a lack of other effective PR:PROJECT_SUMMARY intervention measures except hypothermia therapy, and the effective rate of PR:PROJECT_SUMMARY hypothermia therapy is less than 50%. Using high-throughput metabonomics, we PR:PROJECT_SUMMARY analyzed the serum and brain tissues of 7-day-old HIE mice, mice fed with Acer PR:PROJECT_SUMMARY truncatum Bunge seed oil (ASO) for 30 days and mice fed with their respective PR:PROJECT_SUMMARY ages. All the results revealed that a variety of metabolites changed PR:PROJECT_SUMMARY significantly during injury and repair, and there were many significant PR:PROJECT_SUMMARY enrichment pathways of designed lipid metabolism and neurotrophic factors. PR:PROJECT_SUMMARY Multi-group comparative analysis identified metabolites that may be related to PR:PROJECT_SUMMARY the injury and repair of hypoxic-ischemic encephalopathy, such as glycerol PR:PROJECT_SUMMARY phospholipids and sphingomyelins. Our results suggest that ASO may be a PR:PROJECT_SUMMARY potential special medical food for ischemic hypoxia newborns. PR:INSTITUTE Bao Feng Key Laboratory of Genetics and Metabolism PR:LAST_NAME Chen PR:FIRST_NAME xianyang PR:ADDRESS Room 525, Floor 5, Building 3, Yard 10, Anxiang Street, Airport Economic Core PR:ADDRESS Zone, Shunyi District, Beijing PR:EMAIL cxyibcas@163.com PR:PHONE 13681398052 #STUDY ST:STUDY_TITLE Brain metabonomics of rats in sham, HIE, CO and ASO groups were compared. ST:STUDY_SUMMARY To study the cerebral protective of nervonic acid after hypoxia-ischemia (HI), ST:STUDY_SUMMARY rats were randomly divided into two groups: Sham group (n=12), HIE group (n=8). ST:STUDY_SUMMARY After 48 hours of successful modeling, we re-group the HIE group: CO group (n=5) ST:STUDY_SUMMARY and ASO group (n=8). The ASO group were treated by Acer truncatum Bunge seed oil ST:STUDY_SUMMARY for 30 days, rats in the CO group were treated by an equal volume of corn oil. ST:INSTITUTE Bao Feng Key Laboratory of Genetics and Metabolism ST:LAST_NAME Chen ST:FIRST_NAME xianyang ST:ADDRESS Room 525, Floor 5, Building 3, Yard 10, Anxiang Street, Airport Economic Core ST:ADDRESS Zone, Shunyi District, Beijing ST:EMAIL cxyibcas@163.com ST:PHONE 13681398052 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Rattus norvegicus SU:TAXONOMY_ID 10116 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - 20210630_12 Group:HIE3 Pos or neg=pos; RAW_FILE_NAME=20210630_12.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_14 Group:shaHIE10 Pos or neg=pos; RAW_FILE_NAME=20210630_14.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_16 Group:shaHIE1 Pos or neg=pos; RAW_FILE_NAME=20210630_16.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_18 Group:ASO3 Pos or neg=pos; RAW_FILE_NAME=20210630_18.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_19 Group:shaHIE12 Pos or neg=pos; RAW_FILE_NAME=20210630_19.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_20 Group:shaHIE9 Pos or neg=pos; RAW_FILE_NAME=20210630_20.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_24 Group:CO3 Pos or neg=pos; RAW_FILE_NAME=20210630_24.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_27 Group:CO1 Pos or neg=pos; RAW_FILE_NAME=20210630_27.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_28 Group:ASO1 Pos or neg=pos; RAW_FILE_NAME=20210630_28.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_29 Group:HIE1 Pos or neg=pos; RAW_FILE_NAME=20210630_29.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_33 Group:HIE2 Pos or neg=pos; RAW_FILE_NAME=20210630_33.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_35 Group:shaHIE8 Pos or neg=pos; RAW_FILE_NAME=20210630_35.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_37 Group:CO2 Pos or neg=pos; RAW_FILE_NAME=20210630_37.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_39 Group:shaHIE3 Pos or neg=pos; RAW_FILE_NAME=20210630_39.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_40 Group:shaHIE4 Pos or neg=pos; RAW_FILE_NAME=20210630_40.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_41 Group:shaHIE6 Pos or neg=pos; RAW_FILE_NAME=20210630_41.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_44 Group:shaHIE5 Pos or neg=pos; RAW_FILE_NAME=20210630_44.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_50 Group:shaHIE11 Pos or neg=pos; RAW_FILE_NAME=20210630_50.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_56 Group:ASO5 Pos or neg=pos; RAW_FILE_NAME=20210630_56.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_57 Group:ASO7 Pos or neg=pos; RAW_FILE_NAME=20210630_57.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_59 Group:ASO8 Pos or neg=pos; RAW_FILE_NAME=20210630_59.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_61 Group:HIE4 Pos or neg=pos; RAW_FILE_NAME=20210630_61.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_63 Group:HIE5 Pos or neg=pos; RAW_FILE_NAME=20210630_63.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_64 Group:ASO2 Pos or neg=pos; RAW_FILE_NAME=20210630_64.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_65 Group:shaHIE7 Pos or neg=pos; RAW_FILE_NAME=20210630_65.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_67 Group:shaHIE2 Pos or neg=pos; RAW_FILE_NAME=20210630_67.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_72 Group:HIE8 Pos or neg=pos; RAW_FILE_NAME=20210630_72.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_74 Group:CO4 Pos or neg=pos; RAW_FILE_NAME=20210630_74.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_76 Group:ASO6 Pos or neg=pos; RAW_FILE_NAME=20210630_76.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_77 Group:CO5 Pos or neg=pos; RAW_FILE_NAME=20210630_77.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_78 Group:HIE7 Pos or neg=pos; RAW_FILE_NAME=20210630_78.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_79 Group:ASO4 Pos or neg=pos; RAW_FILE_NAME=20210630_79.mzXML SUBJECT_SAMPLE_FACTORS - 20210630_83 Group:HIE6 Pos or neg=pos; RAW_FILE_NAME=20210630_83.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_12 Group:HIE3 Pos or neg=neg; RAW_FILE_NAME=20210702_12.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_14 Group:shaHIE10 Pos or neg=neg; RAW_FILE_NAME=20210702_14.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_16 Group:shaHIE1 Pos or neg=neg; RAW_FILE_NAME=20210702_16.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_18 Group:ASO3 Pos or neg=neg; RAW_FILE_NAME=20210702_18.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_19 Group:shaHIE12 Pos or neg=neg; RAW_FILE_NAME=20210702_19.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_20 Group:shaHIE9 Pos or neg=neg; RAW_FILE_NAME=20210702_20.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_24 Group:CO3 Pos or neg=neg; RAW_FILE_NAME=20210702_24.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_27 Group:CO1 Pos or neg=neg; RAW_FILE_NAME=20210702_27.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_28 Group:ASO1 Pos or neg=neg; RAW_FILE_NAME=20210702_28.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_29 Group:HIE1 Pos or neg=neg; RAW_FILE_NAME=20210702_29.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_33 Group:HIE2 Pos or neg=neg; RAW_FILE_NAME=20210702_33.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_35 Group:shaHIE8 Pos or neg=neg; RAW_FILE_NAME=20210702_35.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_37 Group:CO2 Pos or neg=neg; RAW_FILE_NAME=20210702_37.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_39 Group:shaHIE3 Pos or neg=neg; RAW_FILE_NAME=20210702_39.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_40 Group:shaHIE4 Pos or neg=neg; RAW_FILE_NAME=20210702_40.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_41 Group:shaHIE6 Pos or neg=neg; RAW_FILE_NAME=20210702_41.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_44 Group:shaHIE5 Pos or neg=neg; RAW_FILE_NAME=20210702_44.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_50 Group:shaHIE11 Pos or neg=neg; RAW_FILE_NAME=20210702_50.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_56 Group:ASO5 Pos or neg=neg; RAW_FILE_NAME=20210702_56.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_57 Group:ASO7 Pos or neg=neg; RAW_FILE_NAME=20210702_57.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_59 Group:ASO8 Pos or neg=neg; RAW_FILE_NAME=20210702_59.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_61 Group:HIE4 Pos or neg=neg; RAW_FILE_NAME=20210702_61.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_63 Group:HIE5 Pos or neg=neg; RAW_FILE_NAME=20210702_63.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_64 Group:ASO2 Pos or neg=neg; RAW_FILE_NAME=20210702_64.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_65 Group:shaHIE7 Pos or neg=neg; RAW_FILE_NAME=20210702_65.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_67 Group:shaHIE2 Pos or neg=neg; RAW_FILE_NAME=20210702_67.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_72 Group:HIE8 Pos or neg=neg; RAW_FILE_NAME=20210702_72.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_74 Group:CO4 Pos or neg=neg; RAW_FILE_NAME=20210702_74.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_76 Group:ASO6 Pos or neg=neg; RAW_FILE_NAME=20210702_76.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_77 Group:CO5 Pos or neg=neg; RAW_FILE_NAME=20210702_77.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_78 Group:HIE7 Pos or neg=neg; RAW_FILE_NAME=20210702_78.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_79 Group:ASO4 Pos or neg=neg; RAW_FILE_NAME=20210702_79.mzXML SUBJECT_SAMPLE_FACTORS - 20210702_83 Group:HIE6 Pos or neg=neg; RAW_FILE_NAME=20210702_83.mzXML #COLLECTION CO:COLLECTION_SUMMARY Take the whole brain according to the experimental purpose, and then immediately CO:COLLECTION_SUMMARY wash the blood residue on the tissue with precooled normal saline or deionized CO:COLLECTION_SUMMARY water. Divide the homogenized brain tissue into sterile tubes for every 250mg of CO:COLLECTION_SUMMARY tissue samples. Take multiple tubes for each sample for backup, mark them, seal CO:COLLECTION_SUMMARY them with sealing membrane, and freeze them at - 80 ℃. CO:SAMPLE_TYPE Brain #TREATMENT TR:TREATMENT_SUMMARY To study the cerebral protective of nervonic acid after hypoxia-ischemia (HI), TR:TREATMENT_SUMMARY rats were randomly divided into two groups: Sham group (n=12), HIE group (n=8). TR:TREATMENT_SUMMARY After 48 hours of successful modeling, we re-group the HIE group: CO group (n=5) TR:TREATMENT_SUMMARY and ASO group (n=8). The ASO group were treated by Acer truncatum Bunge seed oil TR:TREATMENT_SUMMARY for 30 days, rats in the CO group were treated by an equal volume of corn oil25. TR:TREATMENT_SUMMARY Based on the individual recommended intake NA of 0.3 g/kg/d, the equivalent dose TR:TREATMENT_SUMMARY of the drug was calculated according to the bodyweight of the human and the TR:TREATMENT_SUMMARY animal, in which the daily dose of the rat was equivalent to six times that of TR:TREATMENT_SUMMARY adults. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY We use isopropanol (IPA) to extract lipids from serum and brain tissue. In SP:SAMPLEPREP_SUMMARY brief, 100mg of brain tissue homogenized was extracted with 600 μL of precooled SP:SAMPLEPREP_SUMMARY IPA temperature of 4°C, vortexed for 1 minute and incubated for 10 minutes at SP:SAMPLEPREP_SUMMARY room temperature; the extracted mixture was stored overnight at -20 ° C. SP:SAMPLEPREP_SUMMARY Samples were centrifuged for 20 minutes at 14000 rpm, and the supernatants were SP:SAMPLEPREP_SUMMARY transferred into a new centrifuge tube and diluted to 1:10 with IPA/ACN/H2O SP:SAMPLEPREP_SUMMARY (2.5:1:1, v: v: v). Before LC/MS analysis, the sample was stored at -80°C. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY ACQUITYUPLC I-CLASS (Waters) and XEVO-G2XS quadrupole time-of-flight (QTOF) mass CH:CHROMATOGRAPHY_SUMMARY spectrometry (Waters) with ESI were used to analyze the samples. CH:CHROMATOGRAPHY_TYPE UPLC CH:INSTRUMENT_NAME Waters Acquity I-Class CH:COLUMN_NAME Waters Acquity BEH C8 (100 x 2.1mm, 1.7um) CH:METHODS_FILENAME Chrom__methods_.pdf #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Waters Xevo-G2-XS MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS - MS:MS_RESULTS_FILE ST002333_AN003812_Results.txt UNITS:Peak area Has m/z:Yes Has RT:Yes RT units:Minutes #END