#METABOLOMICS WORKBENCH jjacquemyn_20221027_194930 DATATRACK_ID:3541 STUDY_ID:ST002335 ANALYSIS_ID:AN003814 PROJECT_ID:PR001498
VERSION             	1
CREATED_ON             	November 2, 2022, 6:41 am
#PROJECT
PR:PROJECT_TITLE                 	Parkinsonism mutations in DNAJC6 cause lipid defects and neurodegeneration that
PR:PROJECT_TITLE                 	are rescued by Synj1
PR:PROJECT_SUMMARY               	Recent evidence links dysfunctional lipid metabolism to the pathogenesis of
PR:PROJECT_SUMMARY               	Parkinson’s disease, but the mechanisms are not resolved. Here, we generated a
PR:PROJECT_SUMMARY               	new Drosophila knock-in model of DNAJC6/Auxilin and find that the pathogenic
PR:PROJECT_SUMMARY               	mutation causes synaptic dysfunction, neurological defects and
PR:PROJECT_SUMMARY               	neurodegeneration, as well as specific lipid metabolism alterations. In these
PR:PROJECT_SUMMARY               	mutants, membrane lipids containing long-chain polyunsaturated fatty acids,
PR:PROJECT_SUMMARY               	including phosphatidylinositol lipid species that are key for synaptic vesicle
PR:PROJECT_SUMMARY               	recycling and organelle function, are reduced. Overexpression of another protein
PR:PROJECT_SUMMARY               	mutated in Parkinson’s disease, Synaptojanin-1, known to bind and metabolize
PR:PROJECT_SUMMARY               	specific phosphoinositides, rescues the DNAJC6/Auxilin lipid alterations, the
PR:PROJECT_SUMMARY               	neuronal function defects and neurodegeneration. Our work reveals a functional
PR:PROJECT_SUMMARY               	relation between two proteins mutated in Parkinsonism and implicates deregulated
PR:PROJECT_SUMMARY               	phosphoinositide metabolism in the maintenance of neuronal integrity and
PR:PROJECT_SUMMARY               	neuronal survival.
PR:INSTITUTE                     	VIB-KU Leuven
PR:DEPARTMENT                    	Department of Neurosciences
PR:LABORATORY                    	Laboratory of Neuronal Communication - Dr. Patrik Verstreken
PR:LAST_NAME                     	Jacquemyn
PR:FIRST_NAME                    	Julie
PR:ADDRESS                       	Medical Sciences Building 7-25, Edmonton, Alberta, T6H 0L2, Canada
PR:EMAIL                         	jacquemynjulie@ualberta.ca
PR:PHONE                         	5873409325
#STUDY
ST:STUDY_TITLE                   	Pathogenic Auxilin mutations affect lipids that are critical to Synaptojanin
ST:STUDY_TITLE                   	function
ST:STUDY_SUMMARY                 	Mass spectrometry was performed on heads of 15DO flies homogenized in 100 µl
ST:STUDY_SUMMARY                 	D-PBS (Dulbecco’s phosphate-buffered saline without Mg2+ and Ca2+) by
ST:STUDY_SUMMARY                 	Lipotype. Fifteen fly heads were pooled from three independent crosses for each
ST:STUDY_SUMMARY                 	analysis, and mass spectrometry was performed on n=7 for wild-type, n≥4
ST:STUDY_SUMMARY                 	analyses for dAuxWT/WT, dAuxRG/RG, dAuxWT/F956x and dAuxRG/F956x and n=3 for
ST:STUDY_SUMMARY                 	dAuxWT/F956x and dAuxRG/F956x overexpressing Synj.
ST:INSTITUTE                     	VIB-KU Leuven
ST:LAST_NAME                     	Jacquemyn
ST:FIRST_NAME                    	Julie
ST:ADDRESS                       	Medical Sciences Building 7-25, Edmonton, Alberta, T6H 0L2, Canada
ST:EMAIL                         	jacquemynjulie@ualberta.ca
ST:PHONE                         	+1 (587) 3409325
#SUBJECT
SU:SUBJECT_TYPE                  	Invertebrate
SU:SUBJECT_SPECIES               	Drosophila melanogaster
SU:TAXONOMY_ID                   	7227
SU:AGE_OR_AGE_RANGE              	15 day old flies
SU:GENDER                        	Male and female
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	S1	Genotype:AuWT/WT	
SUBJECT_SAMPLE_FACTORS           	-	S2	Genotype:AuWT/WT	
SUBJECT_SAMPLE_FACTORS           	-	S3	Genotype:AuWT/WT	
SUBJECT_SAMPLE_FACTORS           	-	S4	Genotype:AuWT/WT	
SUBJECT_SAMPLE_FACTORS           	-	S5	Genotype:AuxRG/RG	
SUBJECT_SAMPLE_FACTORS           	-	S6	Genotype:AuxRG/RG	
SUBJECT_SAMPLE_FACTORS           	-	S7	Genotype:AuxRG/RG	
SUBJECT_SAMPLE_FACTORS           	-	S8	Genotype:AuxRG/RG	
SUBJECT_SAMPLE_FACTORS           	-	S9	Genotype:AuxRG/RG	
SUBJECT_SAMPLE_FACTORS           	-	S10	Genotype:AuxWT/F956x	
SUBJECT_SAMPLE_FACTORS           	-	S11	Genotype:AuxWT/F956x	
SUBJECT_SAMPLE_FACTORS           	-	S12	Genotype:AuxWT/F956x	
SUBJECT_SAMPLE_FACTORS           	-	S13	Genotype:AuxWT/F956x	
SUBJECT_SAMPLE_FACTORS           	-	S14	Genotype:AuxWT/F956x	
SUBJECT_SAMPLE_FACTORS           	-	S15	Genotype:AuxRG/F956x	
SUBJECT_SAMPLE_FACTORS           	-	S16	Genotype:AuxRG/F956x	
SUBJECT_SAMPLE_FACTORS           	-	S17	Genotype:AuxRG/F956x	
SUBJECT_SAMPLE_FACTORS           	-	S18	Genotype:AuxRG/F956x	
SUBJECT_SAMPLE_FACTORS           	-	S19	Genotype:AuxRG/F956x	
SUBJECT_SAMPLE_FACTORS           	-	S20	Genotype:UAS-synj-AuxWT/F956x	
SUBJECT_SAMPLE_FACTORS           	-	S21	Genotype:UAS-synj-AuxWT/F956x	
SUBJECT_SAMPLE_FACTORS           	-	S22	Genotype:UAS-synj-AuxWT/F956x	
SUBJECT_SAMPLE_FACTORS           	-	S23	Genotype:UAS-synj-AuxRG/F956x	
SUBJECT_SAMPLE_FACTORS           	-	S24	Genotype:UAS-synj-AuxRG/F956x	
SUBJECT_SAMPLE_FACTORS           	-	S25	Genotype:UAS-synj-AuxRG/F956x	
SUBJECT_SAMPLE_FACTORS           	-	S26	Genotype:w1118	
SUBJECT_SAMPLE_FACTORS           	-	S27	Genotype:w1118	
SUBJECT_SAMPLE_FACTORS           	-	S28	Genotype:w1118	
SUBJECT_SAMPLE_FACTORS           	-	S29	Genotype:w1118	
SUBJECT_SAMPLE_FACTORS           	-	S30	Genotype:w1118	
SUBJECT_SAMPLE_FACTORS           	-	S31	Genotype:w1118	
SUBJECT_SAMPLE_FACTORS           	-	S32	Genotype:w1118	
#COLLECTION
CO:COLLECTION_SUMMARY            	Heads of 15DO flies were homogenized in 100 µl D-PBS (Dulbecco’s
CO:COLLECTION_SUMMARY            	phosphate-buffered saline without Mg2+ and Ca2+) and stored at -80°C until
CO:COLLECTION_SUMMARY            	processed for MS analysis by Lipotype. Fifteen fly heads were pooled from three
CO:COLLECTION_SUMMARY            	independent crosses for each analysis, and mass spectrometry was performed on
CO:COLLECTION_SUMMARY            	n=7 for wild-type, n≥4 analyses for dAuxWT/WT, dAuxRG/RG, dAuxWT/F956x and
CO:COLLECTION_SUMMARY            	dAuxRG/F956x and n=3 for dAuxWT/F956x and dAuxRG/F956x overexpressing Synj.
CO:SAMPLE_TYPE                   	Insect tissue
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	In this study we did not apply any additional treatment(s) to the samples.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Lipids were extracted using chloroform and methanol (1). Prior to lipid
SP:SAMPLEPREP_SUMMARY            	extraction, samples were spiked with lipid class-specific internal standards.
SP:SAMPLEPREP_SUMMARY            	After drying and resuspending in an appropriate MS acquisition mixture, lipid
SP:SAMPLEPREP_SUMMARY            	extracts were infused directly in QExactive mass spectrometer (Thermo Fisher
SP:SAMPLEPREP_SUMMARY            	Scientific) with TriVersa NanoMate ion source (Advion Biosciences). Samples are
SP:SAMPLEPREP_SUMMARY            	analyzed in both positive and negative ion modes, with MS resolution
SP:SAMPLEPREP_SUMMARY            	Rm/z=200=280000 and MSMS resolution Rm/z=200=17500, in a single acquisition.
SP:SAMPLEPREP_SUMMARY            	Sample preparations and analysis were done by Lipotype. (1) Sampaio, J. L. et
SP:SAMPLEPREP_SUMMARY            	al. Membrane lipidome of an epithelial cell line. Proceedings of the National
SP:SAMPLEPREP_SUMMARY            	Academy of Sciences 108, 1903–1907 (2011).
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	None (Direct infusion)
CH:INSTRUMENT_NAME               	none
CH:COLUMN_NAME                   	none
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:LABORATORY_NAME               	Lipotype
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Orbitrap
MS:INSTRUMENT_TYPE               	QTRAP
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	Samples are analyzed in both positive and negative ion modes, with MS resolution
MS:MS_COMMENTS                   	Rm/z=200=280000 and MSMS resolution Rm/z=200=17500, in a single acquisition.
MS:MS_COMMENTS                   	Acquired data was processed using lipid identification software based on
MS:MS_COMMENTS                   	LipidXplorer (1). Data post-processing and normalization were performed by
MS:MS_COMMENTS                   	Lipotype using a developed data management system. Data analysis was performed
MS:MS_COMMENTS                   	using GraphPad Prism software 9.3.1. Pmol values (obtained by MS) of individual
MS:MS_COMMENTS                   	lipid species were transformed into a fraction of the total PA, DAG, PI, PC, PE,
MS:MS_COMMENTS                   	and PS lipids in the sample. (1)Herzog, R. et al. Lipidxplorer: A software for
MS:MS_COMMENTS                   	consensual cross-platform lipidomics. PLoS ONE 7, e29851 (2012).
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	pMol
MS_METABOLITE_DATA_START
Samples	S1	S2	S3	S4	S5	S6	S7	S8	S9	S10	S11	S12	S13	S14	S15	S16	S17	S18	S19	S20	S21	S22	S23	S24	S25	S26	S27	S28	S29	S30	S31	S32
Factors	Genotype:AuWT/WT	Genotype:AuWT/WT	Genotype:AuWT/WT	Genotype:AuWT/WT	Genotype:AuxRG/RG	Genotype:AuxRG/RG	Genotype:AuxRG/RG	Genotype:AuxRG/RG	Genotype:AuxRG/RG	Genotype:AuxWT/F956x	Genotype:AuxWT/F956x	Genotype:AuxWT/F956x	Genotype:AuxWT/F956x	Genotype:AuxWT/F956x	Genotype:AuxRG/F956x	Genotype:AuxRG/F956x	Genotype:AuxRG/F956x	Genotype:AuxRG/F956x	Genotype:AuxRG/F956x	Genotype:UAS-synj-AuxWT/F956x	Genotype:UAS-synj-AuxWT/F956x	Genotype:UAS-synj-AuxWT/F956x	Genotype:UAS-synj-AuxRG/F956x	Genotype:UAS-synj-AuxRG/F956x	Genotype:UAS-synj-AuxRG/F956x	Genotype:w1118	Genotype:w1118	Genotype:w1118	Genotype:w1118	Genotype:w1118	Genotype:w1118	Genotype:w1118
DAG 30:0;0	0.00	3.30	10.82	4.52	7.57	3.28	9.65	10.84	9.84	0.00	2.99	10.38	10.04	18.01	5.83	1.77	14.54	10.26	12.17	11.68	0.00	0.00	13.02	8.38	9.23	0.00	0.00	1.57	0.00	9.51	4.82	7.51
DAG 30:1;0	54.02	39.51	201.34	42.06	63.66	29.88	125.97	136.62	181.53	60.18	34.27	251.54	202.39	333.86	57.47	17.46	61.14	74.07	108.26	243.45	320.86	318.31	134.28	90.87	100.84	22.05	29.10	16.64	16.62	224.54	111.83	256.60
DAG 30:2;0	14.47	7.96	35.23	8.59	17.99	4.53	22.37	33.30	43.67	21.24	9.16	71.28	49.13	80.79	23.62	5.16	18.03	21.17	29.00	53.79	68.51	82.78	46.22	37.83	36.29	8.25	9.90	4.16	4.51	60.14	32.23	68.83
DAG 31:0;0	0.00	0.00	0.00	3.48	5.43	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	2.08	0.00	14.67	0.00	3.90	0.00	0.00	0.00	10.16	6.41	7.93	0.00	0.00	1.42	0.00	0.00	0.00	0.00
DAG 31:1;0	5.25	7.98	24.25	16.59	10.50	4.38	25.49	21.24	26.64	0.00	5.13	27.60	21.90	27.18	10.31	2.47	16.87	12.98	23.37	20.88	25.92	26.66	57.49	37.03	42.99	2.94	0.00	2.90	3.72	23.60	20.10	24.77
DAG 31:2;0	0.00	0.00	0.00	4.67	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	2.44	0.00	3.51	0.00	0.00	0.00	0.00	0.00	11.77	7.88	7.13	0.00	0.00	0.00	0.00	0.00	0.00	0.00
DAG 31:3;0	0.00	0.00	0.00	2.32	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
DAG 32:0;0	0.00	0.00	0.00	0.00	4.09	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	2.09	0.00	12.81	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	2.28	0.00	0.00	0.00
DAG 32:1;0	39.05	20.13	109.55	22.31	30.08	14.32	85.57	76.40	101.94	41.68	15.73	170.06	114.21	240.06	28.81	6.52	37.65	44.47	75.92	122.24	168.35	193.37	81.32	46.29	59.95	15.72	23.68	8.76	7.13	168.71	69.34	164.02
DAG 32:2;0	36.71	19.70	70.03	17.47	33.11	8.67	56.47	60.69	83.84	45.63	13.30	153.93	96.68	199.88	32.63	6.33	32.17	40.10	59.55	108.00	112.01	164.50	67.30	52.67	46.82	16.57	25.28	7.29	7.98	158.55	63.42	136.04
DAG 32:3;0	0.00	0.00	0.00	4.38	0.00	0.00	0.00	0.00	5.28	5.87	0.00	8.63	5.84	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	13.73	0.00	3.03	0.00	2.50	3.30	0.00	0.00	11.74	7.07	13.62
DAG 33:0;0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	14.25	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
DAG 33:1;0	0.00	3.84	14.13	11.54	7.60	0.00	14.75	14.49	19.27	0.00	2.57	20.25	14.88	22.18	5.16	1.35	16.02	10.42	20.27	0.00	0.00	13.16	36.90	26.85	28.81	0.00	0.00	1.87	0.00	16.85	11.69	19.62
DAG 33:2;0	0.00	0.00	7.96	7.04	3.16	0.00	6.41	5.71	8.10	0.00	0.00	8.45	6.66	0.00	2.63	0.00	5.72	4.11	5.71	0.00	0.00	0.00	16.78	10.30	12.91	0.00	0.00	0.00	0.00	9.79	7.38	12.99
DAG 34:0;0	0.00	2.15	5.48	0.00	0.00	1.70	5.61	0.00	0.00	0.00	1.87	0.00	0.00	0.00	0.00	1.30	10.94	5.41	0.00	4.62	0.00	0.00	6.39	4.79	5.45	0.00	0.00	1.71	1.48	7.40	0.00	7.25
DAG 34:1;0	17.53	6.49	36.40	7.92	12.45	4.69	41.91	34.68	44.53	22.38	4.26	65.72	41.61	94.49	15.94	2.36	22.62	26.14	46.27	32.20	45.14	61.36	39.33	22.32	28.37	7.20	9.94	3.15	1.83	61.50	23.02	52.43
DAG 34:2;0	24.89	10.07	46.66	12.19	17.43	5.23	39.89	35.70	49.56	27.05	8.02	90.81	54.24	115.43	18.43	3.75	24.72	31.79	51.03	53.21	56.37	79.51	44.72	29.10	35.10	13.13	16.70	4.62	3.63	100.83	42.19	86.04
DAG 34:3;0	10.86	3.37	12.69	3.88	6.74	0.00	8.18	9.79	10.19	14.41	0.00	25.43	14.37	34.50	4.85	0.00	6.33	8.82	9.40	17.32	26.67	36.67	9.56	8.25	5.84	8.49	11.30	2.20	2.35	31.84	14.71	27.66
DAG 35:0;0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	6.71	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
DAG 35:1;0	0.00	0.00	0.00	5.40	3.43	0.00	6.44	4.96	9.27	0.00	0.00	0.00	0.00	0.00	2.88	0.00	9.51	4.85	9.33	0.00	0.00	0.00	14.41	8.48	11.95	0.00	0.00	0.00	0.00	0.00	0.00	0.00
DAG 35:2;0	0.00	0.00	0.00	4.31	0.00	0.00	0.00	0.00	0.00	0.00	0.00	7.88	0.00	0.00	0.00	0.00	4.66	0.00	4.57	0.00	0.00	0.00	10.01	6.90	7.90	0.00	0.00	0.00	0.00	0.00	5.13	7.37
DAG 36:0;0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	6.44	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
DAG 36:1;0	6.19	4.75	10.58	5.03	7.31	0.00	10.38	8.57	42.98	0.00	0.00	16.30	16.00	19.20	7.54	2.44	11.71	6.55	8.62	0.00	0.00	0.00	8.05	5.48	9.84	4.23	4.25	3.38	2.39	16.25	7.12	9.82
DAG 36:2;0	6.81	3.22	13.11	4.34	4.48	2.30	12.99	12.89	16.58	0.00	0.00	24.91	15.48	28.58	7.75	1.83	13.17	14.18	22.66	0.00	0.00	19.20	17.40	12.08	13.93	4.31	5.26	2.43	0.00	26.97	12.58	17.89
DAG 36:3;0	8.81	3.82	10.16	4.35	5.70	2.99	11.96	8.99	9.42	6.02	0.00	23.54	10.39	20.72	5.09	2.04	10.03	10.53	14.67	0.00	0.00	17.98	9.47	11.70	9.20	5.81	6.06	3.10	2.31	22.36	12.22	19.47
DAG 36:4;0	0.00	2.73	6.55	4.03	3.93	2.30	5.54	4.66	0.00	0.00	2.65	10.64	0.00	0.00	3.17	2.12	7.52	6.82	6.51	0.00	0.00	0.00	7.52	7.11	7.55	4.75	4.71	3.09	3.62	10.77	9.91	12.47
PA 24:0;0	1.07	0.00	0.00	0.00	1.11	0.00	0.00	0.00	0.00	1.66	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PA 26:0;0	0.00	0.00	0.00	0.00	2.03	1.19	0.00	0.00	0.00	1.65	1.14	0.00	0.00	0.00	1.47	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	1.14	0.91	0.00	0.00	0.00
PA 26:1;0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	1.29	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PA 28:0;0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.82	0.00	0.00	0.00	0.00
PA 34:0;0	0.50	0.47	0.00	0.00	0.00	0.47	0.00	0.00	0.00	0.00	0.00	0.46	0.48	0.00	0.00	0.55	0.55	0.00	0.91	0.00	0.00	0.56	0.00	0.81	0.00	0.00	0.44	0.39	0.43	0.00	0.00	0.00
PA 34:2;0	1.45	1.27	0.00	0.00	1.72	1.25	0.00	0.00	0.00	1.27	0.00	0.00	0.00	0.00	1.56	1.06	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.90	0.00	0.00	0.00	0.00
PA 36:0;0	1.84	2.03	0.00	0.00	0.00	0.00	0.00	0.00	0.00	2.02	2.07	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	2.38	0.00	1.49	0.00	0.00	0.00
PA 36:2;0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	3.14	3.24	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PA 36:3;0	2.27	1.61	0.00	0.00	1.71	1.51	0.00	0.00	0.00	1.85	1.53	0.00	0.00	0.00	1.76	0.00	0.00	3.19	0.00	0.00	0.00	0.00	0.00	0.00	0.00	1.35	1.65	1.37	1.39	0.00	0.00	0.00
PA 36:4;0	1.87	0.00	0.00	0.00	1.60	1.38	0.00	0.00	0.00	1.89	1.30	0.00	0.00	0.00	1.45	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	1.25	1.13	1.25	1.24	0.00	0.00	0.00
PC 26:0;0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.69	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PC 28:0;0	0.00	0.00	13.91	10.44	0.00	0.00	16.32	17.30	17.38	0.00	0.00	8.71	10.79	12.57	0.00	0.00	15.27	9.81	13.08	7.75	11.04	10.11	0.00	0.00	10.08	0.00	0.00	0.00	0.00	8.55	10.23	0.00
PC 28:1;0	0.00	0.00	8.92	0.00	0.00	0.00	8.54	10.48	9.59	0.00	0.00	7.65	0.00	0.00	0.00	0.00	0.00	10.15	11.90	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PC 30:0;0	6.03	0.00	130.14	82.94	0.00	6.88	127.38	153.80	126.24	3.40	0.00	99.45	89.15	110.36	0.00	0.00	153.86	107.84	147.08	78.76	112.88	106.85	114.57	153.02	131.67	0.00	0.00	0.00	0.00	92.27	101.38	91.85
PC 30:1;0	0.00	0.00	98.05	61.98	0.00	0.00	103.73	125.70	133.40	0.00	0.00	66.99	62.66	63.61	0.00	0.00	93.65	85.60	105.88	56.56	55.00	62.34	67.54	78.67	77.96	0.00	0.00	0.00	0.00	47.45	43.74	45.12
PC 30:2;0	0.00	0.00	8.09	0.00	0.00	3.47	0.00	0.00	0.00	0.00	1.83	0.00	0.00	0.00	5.40	0.00		13.54	14.53	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	1.74	0.00	6.78	0.00	0.00
PC 32:0;0	18.47	13.63	89.63	68.27	19.80	18.14	105.24	120.20	114.43	21.03	17.88	108.63	109.96	137.84	12.95	10.37	145.78	115.90	136.10	90.62	117.21	125.31	135.78	159.19	132.31	13.37	14.50	14.20	14.01	98.13	93.56	92.27
PC 32:1;0	84.32	78.06	278.65	186.57	127.74	104.75	304.94	386.43	373.44	59.47	57.60	265.32	242.94	280.74	89.65	53.82	384.96	378.87	427.74	189.36	198.06	261.65	270.47	282.10	310.46	37.14	42.78	51.86	44.88	226.46	191.86	203.66
PC 32:2;0	84.94	95.82	252.63	180.19	161.37	96.78	284.02	383.08	368.07	65.38	60.19	251.22	218.19	255.69	111.08	53.57	315.40	307.40	392.16	180.05	175.13	234.61	259.54	300.21	294.05	35.47	48.23	52.48	46.04	204.77	164.40	189.00
PC 32:3;0	0.00	0.00	18.86	11.83	0.00	0.00	28.30	25.72	24.22	0.00	0.00	19.16	17.31	16.11	0.00	1.69		38.50	42.07	17.43	16.24	20.24	26.62	31.40	29.83	0.00	0.00	0.00	0.00	0.00	0.00	24.22
PC 34:0;0	22.50	26.16	38.50	27.72	31.74	31.69	44.54	48.19	48.75	23.80	23.11	45.82	43.66	51.28	23.38	17.17	61.71	47.78	58.69	37.36	41.58	44.27	51.90	59.10	50.29	16.80	24.12	17.53	16.96	41.64	44.47	40.12
PC 34:1;0	100.87	79.96	310.17	190.50	150.73	114.73	396.04	468.16	470.95	81.91	67.65	347.84	306.72	380.69	104.65	64.02	550.33	512.98	587.64	219.69	246.92	328.55	347.75	391.36	410.99	60.29	66.98	60.54	57.12	308.55	277.29	288.06
PC 34:2;0	238.43	191.60	579.35	392.42	286.19	200.56	588.74	730.61	723.17	230.26	183.81	644.01	595.84	708.59	202.73	108.93	851.96	796.28	925.33	444.26	477.42	609.43	660.93	759.12	731.06	158.88	181.50	150.04	152.71	612.27	510.73	578.61
PC 34:3;0	105.44	76.97	213.79	132.29	112.31	66.76	198.67	249.05	234.51	102.51	70.05	230.44	211.88	268.13	62.30	37.33	268.01	249.53	271.52	181.31	194.93	257.78	226.32	260.56	248.41	58.80	69.92	57.26	58.98	224.48	195.12	210.76
PC 34:4;0	0.00	0.00	0.00	9.92	0.00	0.00	24.05	24.48	25.95	15.22	11.85	22.68	19.16	22.03	0.00	9.10	29.83	24.17	34.70	17.07	19.46	26.18	31.99	28.89	30.20	8.29	10.43	10.14	9.51	24.67	23.88	23.06
PC 36:1;0	15.37	14.28	45.57	29.99	25.70	25.51	75.27	84.06	91.66	12.58	12.49	56.88	48.95	62.92	16.55	14.76	113.59	97.49	98.28	38.20	43.33	50.44	72.52	72.31	77.09	11.84	12.24	11.59	10.86	54.88	52.80	48.75
PC 36:2;0	76.54	71.96	194.21	126.62	105.48	86.93	222.83	276.61	265.32	63.73	64.09	203.82	185.90	230.31	70.56	45.76	331.59	313.08	345.74	113.77	132.84	167.36	222.77	244.30	238.17	57.86	59.27	52.49	54.80	185.51	178.13	179.78
PC 36:3;0	151.36	127.48	260.86	175.09	182.61	135.57	271.94	333.86	326.38	151.04	114.81	304.40	274.22	357.65	107.08	78.35	393.06	344.19	379.65	192.46	222.70	285.89	295.79	345.44	323.30	121.00	135.51	106.16	106.93	301.71	265.84	293.93
PC 36:4;0	107.79	84.65	160.03	120.73	106.78	78.30	141.16	156.87	162.53	133.19	100.04	178.21	190.18	237.02	55.61	41.02	175.68	147.16	155.36	165.64	182.78	216.45	182.93	203.61	178.01	99.84	116.30	79.78	89.20	217.70	194.68	225.26
PC 36:5;0	0.00	0.00	0.00	10.98	0.00	0.00	0.00	0.00	0.00	52.79	37.11	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	26.44	26.96	0.00	33.48	36.51	29.49	29.86	0.00	24.27	0.00
PE 26:0;0	0.00	0.00	3.84	2.28	0.00	0.00	2.37	3.36	3.46	0.00	0.00	4.55	2.39	2.40	4.45	0.00	3.06	4.28	5.40	0.00	0.00	0.00	4.47	3.90	5.51	0.00	0.00	0.00	0.00	1.87	0.00	2.15
PE 28:0;0	1.97	0.00	9.60	6.97	0.00	3.05	8.87	11.37	11.09	0.00	0.00	6.00	4.26	4.07	0.00	0.00	4.85	4.45	4.63	4.38	3.68	3.29	3.77	4.43	3.98	0.00	0.00	0.00	0.00	3.01	2.57	2.73
PE 28:1;0	0.00	0.00	3.76	2.31	0.00	2.15	4.15	4.36	4.35	0.00	0.00	6.11	3.49	3.87	5.18	1.54	3.73	4.07	4.49	3.27	3.26	2.83	4.76	5.19	6.02	0.00	0.00	0.00	0.00	0.00	2.34	0.00
PE 30:0;0	2.65	2.13	12.73	8.27	3.90	2.34	9.46	11.89	12.48	0.00	1.53	6.61	6.56	8.20	0.00	1.38	11.26	10.25	9.71	5.77	5.92	6.37	6.55	6.57	6.14	0.00	0.00	0.00	0.00	4.56	4.43	4.83
PE 30:1;0	22.37	27.49	76.32	49.42	43.80	40.89	82.83	92.27	103.53	9.53	27.92	89.37	65.81	61.06	88.23	35.14	76.65	90.35	94.55	75.49	65.42	54.70	98.63	99.33	115.19	5.70	7.19	14.97	14.98	40.51	43.33	41.46
PE 30:2;0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	2.40	0.00	0.00	2.74	0.00	0.00	2.27	0.00	0.00	0.00	2.77	0.00	0.00	0.00	2.95	0.00	3.21	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PE 31:0;0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	3.01	2.76	0.00	0.00	0.00	0.00	0.00	0.00	0.00	5.04	4.16	5.36	0.00	0.00	0.00	3.64	5.02	4.33	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PE 31:1;0	2.23	4.77	7.75	5.00	5.36	5.87	9.29	12.51	11.48	0.00	3.42	6.89	5.30	5.29	11.68	3.61	9.50	13.21	13.54	9.36	7.13	6.49	17.24	17.35	18.30	0.00	0.00	2.77	2.17	5.16	6.77	5.81
PE 32:0;0	4.20	0.00	11.73	8.69	3.87	0.00	9.89	11.36	13.14	3.33	2.27	9.77	8.70	12.41	0.00	0.00	13.18	12.24	12.78	8.79	10.14	10.92	11.20	10.10	12.36	1.73	1.54	1.65	1.80	9.42	10.35	9.79
PE 32:1;0	194.76	188.63	355.44	213.97	353.64	260.61	495.74	556.47	609.47	122.61	178.54	545.08	401.90	470.03	372.00	165.89	416.38	511.09	559.68	363.28	395.89	431.44	500.10	487.33	513.03	72.40	85.99	116.16	104.50	274.76	230.06	251.72
PE 32:2;0	65.35	67.75	161.18	113.95	122.05	82.86	211.56	250.46	264.96	45.91	54.96	218.95	163.64	190.06	144.86	54.68	205.09	238.57	265.84	146.64	142.47	155.20	233.75	253.04	273.83	24.48	31.57	46.10	36.63	149.34	141.32	145.89
PE 32:3;0	2.56	0.00	0.00	0.00	2.80	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PE 33:0;0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	2.66	3.57	0.00	0.00	2.69	0.00	0.00	0.00	0.00	5.71	7.00	5.09	3.36		2.54	4.55	3.24	4.62	0.00	0.00	0.00	0.00	3.37	2.64	3.26
PE 33:1;0	6.79	8.53	18.17	12.43	12.56	11.27	26.56	29.87	32.69	4.53	6.18	20.89	16.41	19.31	23.26	7.97	40.36	44.37	45.06	17.37	18.66	16.78	43.16	45.32	47.72	2.52	2.43	5.67	5.89	17.47	16.79	15.31
PE 33:2;0	3.78	5.07	11.07	7.58	5.59	7.14	13.07	15.10	15.10	3.58	4.24	11.24	10.58	10.86	11.27	3.70	16.37	20.27	21.51	8.41	8.45	9.58	17.02	18.51	19.79	2.09	2.13	2.94	3.22	8.79	9.27	10.01
PE 34:1;0	252.62	174.60	540.12	351.53	388.61	199.99	656.56	764.49	758.30	191.00	147.80	674.88	515.67	676.22	364.51	138.27	901.79	970.82	1024.09	400.91	452.30	544.66	729.46	732.02	766.29	136.35	146.07	123.49	116.76	496.64	435.02	459.72
PE 34:2;0	339.92	237.75	678.40	462.38	452.32	266.27	792.21	946.84	976.11	317.15	256.67	941.48	761.47	966.03	361.92	180.89	1072.13	1063.27	1108.65	647.98	700.69	823.45	910.98	994.60	1024.62	206.00	236.45	206.24	193.42	800.22	709.21	766.21
PE 34:3;0	131.81	86.11	254.39	173.03	148.95	91.12	284.67	327.22	339.18	124.76	84.00	340.13	280.25	366.99	109.50	54.92	355.75	349.74	357.92	230.09	243.01	287.85	320.31	343.96	334.36	75.25	88.22	73.32	70.48	298.54	271.29	298.22
PE 34:4;0	4.54	0.00	4.10	0.00	6.70	0.00	0.00	0.00	4.30	4.36	0.00	10.12	6.80	12.75	0.00	0.00	0.00	0.00	5.66	8.92	8.64	7.61	12.17	0.00	6.43	0.00	0.00	0.00	0.00	6.37	4.99	8.78
PE 35:1;0	7.88	7.66	21.42	13.33	12.10	9.98	27.40	27.34	30.43	6.19	5.18	20.58	16.07	21.82	15.28	7.40	49.33	50.62	51.68	19.77	18.66	20.82	44.52	42.40	47.48	4.30	4.44	5.80	5.47	21.11	22.22	23.60
PE 35:2;0	10.93	10.55	28.49	18.28	12.78	11.66	32.07	37.69	37.68	9.94	9.06	30.71	25.91	34.54	18.29	9.64	62.14	60.87	63.48	25.95	31.01	30.71	51.88	52.85	54.05	7.85	8.10	8.40	8.65	34.21	31.61	35.04
PE 35:3;0	4.33	3.98	11.74	7.84	0.00	4.17	11.77	13.61	14.79	4.33	3.79	14.08	12.83	15.37	6.17	2.64	22.34	20.86	22.86	10.16	11.36	13.03	16.68	19.04	19.21	3.27	2.82	3.04	2.96	14.04	12.74	14.37
PE 36:1;0	54.24	33.53	154.58	96.42	62.02	45.84	181.69	202.91	200.65	38.99	33.24	168.15	138.50	175.61	47.01	29.41	243.48	253.43	251.63	111.92	124.37	140.42	172.52	178.46	190.48	35.18	36.45	29.98	32.31	169.53	161.29	164.66
PE 36:2;0	368.04	249.96	784.52	549.72	437.15	320.38	973.32	1094.55	1122.78	322.35	254.56	1024.64	890.24	1107.46	337.84	207.21	1394.90	1363.09	1379.55	726.77	801.59	888.02	1083.69	1150.64	1188.49	232.19	242.92	214.78	216.90	867.55	812.50	879.14
PE 36:3;0	267.76	180.24	491.28	351.39	292.24	212.04	573.19	652.38	666.83	290.78	216.75	670.75	568.06	765.91	204.99	132.39	833.31	791.51	761.98	513.07	564.58	641.30	725.19	767.70	762.46	199.25	210.14	175.07	179.29	619.73	607.73	638.80
PE 36:4;0	192.29	133.43	466.18	344.52	175.32	142.06	443.15	467.16	496.60	231.24	191.36	605.75	568.21	754.49	120.63	84.27	653.50	566.84	557.06	498.87	545.72	605.22	609.17	639.82	612.31	152.99	166.47	136.71	147.32	564.12	557.96	611.89
PE 36:5;0	40.19	29.74	105.77	89.83	33.30	36.50	107.96	108.73	117.60	52.30	52.75	143.02	141.65	181.32	22.76	18.12	138.83	121.81	122.94	117.90	123.87	141.50	162.68	157.17	147.09	32.53	32.25	32.37	35.30	129.80	138.21	149.99
PE 36:6;0	5.09	3.72	10.35	10.42	3.68	3.52	11.87	10.50	12.02	5.67	5.93	14.39	15.97	18.57	0.00	2.22	13.42	10.45	10.39	11.52	13.09	13.54	20.35	16.02	15.27	3.16	3.38	3.49	4.50	15.06	14.60	18.46
PE 37:1;0	0.00	0.00	3.41	2.89	0.00	0.00	4.57	5.36	5.28	0.00	0.00	4.16	3.37	4.99	0.00	0.00	11.02	10.89	11.18	3.58	5.04	5.15	6.48	6.82	7.06	0.00	0.00	0.00	0.00	6.78	5.79	6.08
PE 37:2;0	0.00	0.00	4.61	3.18	0.00	2.40	6.38	5.19	7.13	0.00	0.00	4.97	5.70	6.36	4.00	1.69	11.04	11.84	11.97	5.27	4.46	5.20	8.61	8.47	9.74	0.00	0.00	0.00	0.00	7.03	6.61	5.65
PE 37:3;0	0.00	0.00	2.87	0.00	0.00	0.00	3.32	0.00	3.73	0.00	0.00	2.80	2.56	3.48	0.00	0.00	4.68	5.04	5.28	2.46	0.00	2.96	4.07	5.32	4.90	0.00	0.00	0.00	0.00	2.48	3.46	2.83
PE 38:1;0	4.06	0.00	13.25	7.54	0.00	3.96	12.40	13.76	16.73	3.03	2.39	16.85	12.76	14.93	4.73	2.60	23.17	27.19	25.78	10.45	12.38	12.47	16.97	18.14	16.38	3.26	3.50	2.30	2.96	20.92	21.26	20.94
PE 38:2;0	7.62	4.43	14.82	9.75	6.36	6.54	15.85	17.41	21.17	6.31	5.00	20.58	19.03	23.12	7.96	5.02	31.13	29.51	28.53	13.81	15.46	17.99	25.37	24.99	23.23	5.63	6.01	4.27	4.50	20.45	18.71	19.32
PE 38:3;0	4.14	0.00	5.88	4.04	0.00	2.81	7.39	7.18	7.34	3.30	0.00	8.59	7.38	10.63	2.67	0.00	11.16	11.52	10.40	6.44	6.56	8.45	10.81	11.27	8.72	2.39	3.00	1.98	1.93	7.84	7.16	8.63
PG 30:1;0	0.00	0.00	2.24	1.44	0.00	0.00	2.68	3.41	2.82	0.00	0.00	1.41	0.00	0.00	0.00	0.00	2.05	1.82	0.00	0.00	0.00	0.00	2.12	2.34	2.11	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PG 30:2;0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	2.32	2.29
PG 32:0;0	0.00	0.00	1.58	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	1.62	0.00	2.09	0.00	0.00	0.00	0.00	1.84	1.69	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PG 32:1;0	2.93	2.47	9.36	6.73	3.81	2.60	10.94	14.17	12.57	2.25	2.13	10.01	10.51	11.29	2.79	1.54	12.16	13.10	14.51	8.82	9.19	10.86	13.10	12.12	11.82	1.04	1.37	1.86	1.50	9.27	8.31	8.61
PG 32:2;0	3.54	2.64	15.85	11.23	4.59	3.02	19.39	23.34	22.40	3.32	2.73	17.56	18.09	18.66	3.80	1.96	23.68	20.57	26.08	11.46	12.46	17.54	24.34	24.15	26.00	2.03	2.04	2.33	2.20	16.81	16.64	15.71
PG 32:3;0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	7.53	5.81	0.00	0.00	0.00	5.94	6.02	0.00	0.00	7.46	0.00	0.00	3.69	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PG 34:0;0	0.00	0.00	0.00	1.44	0.00	0.56	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.67	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PG 34:1;0	3.55	2.81	13.12	8.94	2.67	2.25	10.29	11.79	11.74	2.78	1.96	15.54	15.60	16.97	1.91	1.16	22.90	22.57	23.63	6.22	6.92	8.39	10.70	11.38	11.43	1.19	1.20	1.50	1.30	8.73	8.25	8.01
PG 34:2;0	9.52	6.28	37.78	27.76	9.53	6.79	42.06	52.83	48.26	10.80	7.60	40.68	43.97	47.14	8.36	4.76	54.47	47.52	53.31	31.21	34.62	43.89	55.02	55.49	60.28	6.02	7.57	6.69	6.46	44.83	43.13	44.51
PG 34:3;0	3.93	2.66	12.48	9.14	4.05	2.57	16.12	19.94	17.29	3.96	2.68	14.15	13.45	15.05	2.97	1.99	17.91	16.43	19.31	10.52	11.79	15.40	18.59	22.14	20.43	2.11	2.94	2.33	2.24	15.33	14.38	14.92
PG 35:3;0	1.51	1.26	0.00	0.00	1.50	1.65	0.00	0.00	0.00	0.00	1.52	0.00	0.00	0.00	1.26	1.09	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	1.29	0.00	1.66	0.00	0.00	0.00
PG 36:2;0	2.05	1.56	9.99	7.26	2.03	1.98	9.63	12.60	11.78	2.16	1.57	11.80	12.63	13.27	1.31	1.24	15.69	15.76	16.42	6.49	8.17	8.79	13.50	12.16	12.40	1.17	1.19	1.36	1.30	11.12	11.02	11.16
PG 36:3;0	4.12	2.80	10.33	7.38	4.32	3.18	12.11	16.05	14.70	5.10	3.26	13.64	12.43	15.50	3.27	1.79	16.94	14.22	14.42	8.76	11.71	15.68	14.58	17.52	15.86	2.75	3.37	3.16	2.84	13.34	12.00	13.53
PG 36:4;0	3.37	2.52	6.72	5.40	2.69	2.46	7.88	9.50	9.84	4.11	3.45	9.24	9.73	12.42	2.32	1.49	11.69	9.32	9.64	8.36	8.72	11.70	11.19	12.53	11.69	2.21	3.46	2.06	2.66	8.50	8.94	9.81
PI 30:0;0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	1.72	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PI 30:1;0	0.00	0.00	7.34	3.72	5.24	4.18	13.60	19.11	17.39	0.00	0.00	4.18	4.31	3.58	2.02	1.42	10.97	9.36	11.70	2.83	2.25	3.75	3.28	4.64	7.21	0.00	0.00	0.00	0.00	1.94	1.91	0.00
PI 30:2;0	0.00	2.60	0.00	0.00	0.00	2.58	0.00	0.00	0.00	0.00	1.69	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PI 31:0;0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	1.51	0.00	0.00	0.00	0.00	0.00	0.00	0.00	2.33	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PI 31:1;0	0.00	0.00	0.00	1.15	0.00	0.00	2.83	4.31	3.60	0.00	0.00	0.00	0.00	0.00	0.00	0.00	3.37	3.12	3.08	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PI 32:0;0	0.00	0.96	1.44	0.96	0.79	0.00	1.55	2.03	1.85	0.47	0.00	0.00	1.78	1.93	0.71	0.00	2.18	2.18	2.05	1.50	1.43	1.83	1.87	1.90	2.24	0.00	0.00	0.00	0.00	0.00	1.52	0.00
PI 32:1;0	14.03	13.83	56.40	36.49	29.27	21.26	72.61	88.37	85.77	6.75	8.24	39.91	41.15	48.13	13.66	7.39	69.51	76.35	90.30	21.51	23.11	33.05	30.98	29.97	48.50	3.87	5.83	6.45	5.41	18.18	10.18	13.51
PI 32:2;0	0.00	0.00	34.81	24.96	17.51	6.71	47.23	62.01	55.55	0.00	0.00	30.01	30.97	39.74	5.39	0.00	58.54	48.40	62.80	20.77	17.98	31.46	31.00	32.93	40.36	0.00	0.00	0.00	0.00	14.38	11.50	12.40
PI 33:0;0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	2.88	1.60	2.21	0.00	0.00	0.00	2.30	0.00	2.97	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PI 33:1;0	0.00	0.00	3.94	2.47	1.94	1.91	5.84	8.02	6.21	0.00	0.00	2.65	3.09	3.67	1.46	0.00	10.96	7.92	9.93	2.35	2.26	0.00	6.85	6.13	9.13	0.00	0.00	0.00	0.00	3.10	2.96	2.03
PI 33:2;0	0.00	0.00	2.34	1.81	0.00	0.00	2.69	3.99	3.28	0.00	0.00	0.00	1.91	0.00	0.00	0.00	5.56	4.26	5.72	0.00	0.00	0.00	3.22	3.03	4.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PI 34:0;0	0.00	0.00	1.86	1.28	0.00	0.00	2.79	2.97	2.97	0.00	0.00	1.81	3.03	4.47	0.00	0.00	4.40	3.48	3.57	2.31	2.64	3.46	4.56	4.37	4.04	0.00	0.00	0.00	0.00	3.71	3.26	2.71
PI 34:1;0	9.25	7.72	42.67	27.69	18.04	11.70	56.87	69.00	64.64	5.20	5.00	40.39	43.28	58.32	12.65	5.29	94.71	78.98	100.28	24.09	23.96	39.76	53.30	53.77	67.25	3.68	4.33	4.34	3.78	38.12	29.85	31.14
PI 34:2;0	29.45	23.54	109.50	79.56	51.10	29.22	111.11	141.61	129.18	23.10	18.11	97.08	107.55	147.03	27.55	12.64	202.10	156.74	179.02	78.54	72.86	115.41	125.21	125.54	155.75	12.15	15.17	15.00	14.62	89.80	77.59	79.45
PI 34:3;0	15.12	9.99	32.89	23.13	21.53	9.45	28.67	39.06	35.56	12.06	7.81	35.99	35.50	55.40	8.79	4.79	60.29	43.32	43.93	32.18	27.38	48.61	43.15	42.34	42.94	6.36	7.72	6.89	6.80	32.71	26.60	30.24
PI 34:4;0	0.00	0.00	3.11	2.01	2.06	1.36	2.77	2.71	3.17	0.00	0.00	2.47	3.53	4.81	0.00	0.00	5.74	3.58	4.33	2.92	2.06	3.65	4.45	4.84	5.41	0.00	0.00	0.00	0.00	2.11	2.62	2.01
PI 35:1;0	0.00	0.00	2.12	1.31	0.00	0.00	3.18	3.23	3.13	0.00	0.00	1.64	1.83	2.78	1.56	0.00	6.51	5.49	4.99	0.00	0.00	0.00	4.29	3.31	4.95	0.00	0.00	0.00	0.00	0.00	1.77	0.00
PI 35:2;0	1.88	1.99	5.00	3.66	2.81	2.22	5.95	6.33	5.92	1.59	1.28	3.58	4.17	6.15	2.28	1.43	13.44	10.41	11.52	3.90	3.37	4.59	9.77	9.61	10.59	0.00	0.00	1.31	0.00	5.03	4.70	4.49
PI 35:3;0	7.18	6.56	2.04	1.38	6.27	5.01	1.89	0.00	1.80	5.53	5.46	0.00	0.00	0.00	5.43	4.38	5.59	3.92	3.41	0.00	0.00	0.00	3.60	3.44	5.07	6.61	5.67	4.26	4.62	0.00	0.00	0.00
PI 36:1;0	0.00	0.00	6.61	3.97	2.15	1.55	8.58	9.56	8.60	0.00	0.00	4.76	5.01	7.14	0.00	0.00	18.79	13.04	15.12	2.69	2.93	3.64	7.38	7.48	8.76	0.00	0.00	0.00	0.00	4.60	5.37	4.35
PI 36:2;0	16.79	13.09	65.88	45.62	24.86	16.12	65.94	80.12	71.97	12.98	10.56	51.75	63.15	86.84	14.86	8.20	141.49	104.19	115.14	43.97	43.09	61.75	88.69	91.13	105.09	9.74	9.81	8.68	9.51	61.28	51.78	54.48
PI 36:3;0	46.26	34.49	151.34	109.86	63.44	40.44	132.87	169.95	164.03	39.71	30.80	134.26	158.25	222.96	32.50	21.17	312.79	205.54	215.47	124.21	115.36	180.36	187.22	204.53	222.29	29.16	31.56	25.65	26.58	163.26	144.03	145.16
PI 36:4;0	34.89	25.65	78.43	57.94	35.15	27.94	69.05	85.30	84.27	34.24	28.93	80.10	105.99	140.59	17.80	14.80	156.14	96.40	101.83	92.21	85.64	127.60	125.93	132.62	134.23	25.94	29.80	22.49	25.21	108.68	100.71	103.01
PI 36:5;0	10.45	7.40	13.17	9.57	8.34	8.03	12.40	14.20	13.68	11.97	10.03	17.30	22.75	33.99	4.57	4.21	31.95	17.54	18.15	21.19	19.56	28.07	34.41	33.12	34.74	8.17	10.07	8.17	9.25	21.31	22.05	20.75
PI 36:6;0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	1.27	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PS 32:1;0	0.00	0.00	4.36	3.28	3.04	2.30	4.96	5.35	5.05	0.00	0.00	3.65	2.33	2.97	0.00	0.00	7.20	7.89	7.58	2.13	0.00	2.54	3.85	4.59	4.22	0.00	0.00	0.00	0.00	2.31	2.06	2.65
PS 32:2;0	0.00	0.00	3.66	2.46	2.26	0.00	3.76	4.83	3.85	0.00	0.00	2.57	1.66	2.40	0.00	0.00	5.54	5.95	5.05	0.00	0.00	2.09	2.91	2.88	2.36	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PS 32:3;0	0.00	0.00	4.57	2.99	0.00	0.00	5.29	5.85	6.05	0.00	0.00	5.00	3.74	4.32	0.00	0.00	4.84	4.65	6.59	5.13	4.01	3.35	6.54	5.49	7.89	0.00	0.00	0.00	0.00	2.83	2.89	2.68
PS 33:1;0	0.00	0.00	0.00	0.00	0.00	2.05	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	2.50	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PS 34:0;0	1.08	1.00	3.10	2.35	1.24	0.90	3.22	3.91	3.61	1.11	0.73	2.46	0.00	3.79	0.00	0.86	4.99	4.95	4.70	2.65	2.87	3.35	3.98	4.22	4.39	1.18	0.98	1.01	1.10	3.94	3.32	3.15
PS 34:1;0	9.26	8.28	15.11	7.95	12.07	9.69	17.64	19.49	19.00	7.10	6.58	14.58	11.74	13.55	10.19	6.04	33.06	34.45	30.15	11.55	9.76	13.82	18.40	21.77	22.47	5.87	5.94	5.64	4.81	15.38	13.38	11.10
PS 34:2;0	15.88	11.95	31.25	19.66	25.56	15.34	35.12	40.38	37.47	9.88	9.82	27.36	21.94	28.23	15.00	9.94	57.52	56.73	54.69	23.16	23.05	25.67	30.52	37.59	34.06	6.49	8.23	9.15	7.51	23.42	22.39	18.17
PS 34:3;0	7.55	6.63	25.48	16.20	9.92	7.34	30.60	37.88	41.22	5.99	5.12	34.36	27.67	34.34	6.05	4.13	34.51	36.40	43.46	26.36	26.25	32.11	39.08	33.57	36.37	5.33	5.85	4.93	4.37	24.22	19.94	18.07
PS 34:4;0	0.00	0.00	10.49	7.04	0.00	2.62	11.84	15.88	15.18	0.00	0.00	13.50	10.48	12.44	0.00	0.00	14.62	13.45	17.28	9.31	9.12	11.20	16.77	15.28	19.51	0.00	0.00	0.00	0.00	10.22	9.79	9.63
PS 35:1;0	0.00	0.00	0.00	0.00	4.42	2.42	0.00	0.00	0.00	0.00	2.06	0.00	0.00	0.00	4.11	1.85	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	1.61	0.00	0.00	0.00	0.00
PS 35:2;0	4.32	3.10	0.00	0.00	4.40	3.42	0.00	0.00	0.00	3.30	3.40	0.00	0.00	0.00	4.50	2.94	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	3.71	2.97	2.71	2.26	0.00	0.00	0.00
PS 36:0;0	0.00	0.00	6.70	4.47	0.00	0.00	8.52	10.93	11.12	0.00	0.00	6.47	5.48	6.25	0.00	0.00	13.31	14.47	15.15	5.22	5.82	6.49	10.17	11.15	11.54	0.00	0.00	0.00	0.00	6.38	6.40	6.23
PS 36:1;0	12.65	8.01	19.81	11.18	14.65	10.81	27.33	29.80	30.27	9.43	7.77	19.45	16.99	21.48	11.20	7.30	47.99	47.01	44.29	17.15	17.36	19.58	32.80	34.69	35.45	8.88	8.82	6.58	7.17	24.88	23.48	19.48
PS 36:2;0	72.10	56.59	97.62	54.67	98.17	71.40	129.45	163.34	154.85	55.00	49.49	113.88	87.92	117.41	66.77	43.97	241.86	235.02	224.69	97.57	95.31	114.18	159.60	185.77	174.61	45.33	48.21	40.81	42.27	109.78	104.73	94.63
PS 36:3;0	53.57	38.06	78.47	52.45	53.57	43.28	90.13	111.28	106.91	45.84	38.95	90.40	75.37	97.19	40.16	28.66	158.88	149.32	144.39	85.37	85.63	98.35	132.76	144.46	136.68	38.54	41.51	32.67	34.97	98.31	92.41	81.91
PS 36:4;0	53.44	39.35	88.69	55.09	52.46	44.67	93.79	121.06	120.29	41.66	40.04	112.45	90.72	123.64	35.65	26.81	179.75	158.25	153.20	103.58	100.29	118.98	139.94	158.49	152.64	36.36	42.84	33.83	34.52	120.98	110.56	101.38
PS 36:5;0	59.36	44.98	47.74	29.55	52.60	50.76	55.91	70.37	70.14	46.62	48.49	64.15	57.74	73.09	36.55	28.64	110.50	99.81	83.00	70.83	68.83	79.51	103.97	114.48	108.44	41.07	48.11	39.96	40.06	75.77	73.76	61.13
PS 36:6;0	24.39	17.24	8.73	5.70	19.60	22.71	13.42	15.13	15.43	20.27	21.12	13.68	12.74	15.22	12.68	10.59	21.39	18.97	16.06	17.71	16.99	16.77	31.03	29.42	28.86	15.39	20.09	16.19	16.34	16.03	16.35	13.86
PS 37:2;0	0.00	0.00	0.00	0.00	4.55	0.00	0.00	0.00	0.00	0.00	2.85	0.00	0.00	0.00	4.20	2.34	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	3.11	0.00	0.00	2.43	0.00	0.00	0.00
PS 38:0;0	0.00	0.00	3.70	0.00	0.00	0.00	4.70	0.00	5.60	0.00	0.00	0.00	0.00	3.30	0.00	0.00	9.11	9.01	8.25	0.00	3.45	0.00	5.94	5.80	6.19	0.00	0.00	0.00	0.00	3.97	4.37	4.10
PS 38:1;0	0.00	0.00	8.33	4.41	0.00	0.00	8.74	10.34	9.49	0.00	0.00	6.78	8.39	9.41	0.00	0.00	17.32	18.43	16.76	8.05	6.95	8.24	13.50	13.46	13.68	0.00	0.00	0.00	0.00	11.22	10.91	9.44
PS 38:2;0	5.51	4.23	5.49	3.13	6.56	4.92	5.92	7.63	6.44	4.74	4.25	5.84	5.57	6.24	5.52	3.44	13.36	12.69	11.62	5.30	5.22	6.49	9.50	12.47	9.66	4.86	4.61	4.17	3.61	7.91	6.88	6.16
PS 38:3;0	105.70	0.00	9.71	6.06	91.66	70.28	10.68	11.14	10.95	80.19	84.27	9.19	8.35	11.75	86.59	71.21	15.18	15.17	17.01	6.76	7.44	8.89	12.73	10.92	12.47	92.94	100.47	74.38	80.76	12.70	10.06	9.70
PS 38:4;0	0.00	0.00	42.47	0.00	0.00	0.00	0.00	0.00	61.69	0.00	0.00	54.26	0.00	62.81	0.00	0.00	71.62	65.35	71.19	0.00	0.00	52.79	0.00	60.43	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
PS 38:7;0	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	4.81	0.00	0.00	0.00	0.00	0.00	2.87	0.00	0.00	0.00	0.00	0.00	13.75	0.00	15.82	16.49	0.00	0.00	0.00	0.00	12.95	0.00	0.00
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	Class	Structural category	Functional category	Total length	Total db	Totaloh
DAG 30:0;0	DAG	GL	MEM	30	0	0
DAG 30:1;0	DAG	GL	MEM	30	1	0
DAG 30:2;0	DAG	GL	MEM	30	2	0
DAG 31:0;0	DAG	GL	MEM	31	0	0
DAG 31:1;0	DAG	GL	MEM	31	1	0
DAG 31:2;0	DAG	GL	MEM	31	2	0
DAG 31:3;0	DAG	GL	MEM	31	3	0
DAG 32:0;0	DAG	GL	MEM	32	0	0
DAG 32:1;0	DAG	GL	MEM	32	1	0
DAG 32:2;0	DAG	GL	MEM	32	2	0
DAG 32:3;0	DAG	GL	MEM	32	3	0
DAG 33:0;0	DAG	GL	MEM	33	0	0
DAG 33:1;0	DAG	GL	MEM	33	1	0
DAG 33:2;0	DAG	GL	MEM	33	2	0
DAG 34:0;0	DAG	GL	MEM	34	0	0
DAG 34:1;0	DAG	GL	MEM	34	1	0
DAG 34:2;0	DAG	GL	MEM	34	2	0
DAG 34:3;0	DAG	GL	MEM	34	3	0
DAG 35:0;0	DAG	GL	MEM	35	0	0
DAG 35:1;0	DAG	GL	MEM	35	1	0
DAG 35:2;0	DAG	GL	MEM	35	2	0
DAG 36:0;0	DAG	GL	MEM	36	0	0
DAG 36:1;0	DAG	GL	MEM	36	1	0
DAG 36:2;0	DAG	GL	MEM	36	2	0
DAG 36:3;0	DAG	GL	MEM	36	3	0
DAG 36:4;0	DAG	GL	MEM	36	4	0
PA 24:0;0	PA	GPL	MEM	24	0	0
PA 26:0;0	PA	GPL	MEM	26	0	0
PA 26:1;0	PA	GPL	MEM	26	1	0
PA 28:0;0	PA	GPL	MEM	28	0	0
PA 34:0;0	PA	GPL	MEM	34	0	0
PA 34:2;0	PA	GPL	MEM	34	2	0
PA 36:0;0	PA	GPL	MEM	36	0	0
PA 36:2;0	PA	GPL	MEM	36	2	0
PA 36:3;0	PA	GPL	MEM	36	3	0
PA 36:4;0	PA	GPL	MEM	36	4	0
PC 26:0;0	PC	GPL	MEM	26	0	0
PC 28:0;0	PC	GPL	MEM	28	0	0
PC 28:1;0	PC	GPL	MEM	28	1	0
PC 30:0;0	PC	GPL	MEM	30	0	0
PC 30:1;0	PC	GPL	MEM	30	1	0
PC 30:2;0	PC	GPL	MEM	30	2	0
PC 32:0;0	PC	GPL	MEM	32	0	0
PC 32:1;0	PC	GPL	MEM	32	1	0
PC 32:2;0	PC	GPL	MEM	32	2	0
PC 32:3;0	PC	GPL	MEM	32	3	0
PC 34:0;0	PC	GPL	MEM	34	0	0
PC 34:1;0	PC	GPL	MEM	34	1	0
PC 34:2;0	PC	GPL	MEM	34	2	0
PC 34:3;0	PC	GPL	MEM	34	3	0
PC 34:4;0	PC	GPL	MEM	34	4	0
PC 36:1;0	PC	GPL	MEM	36	1	0
PC 36:2;0	PC	GPL	MEM	36	2	0
PC 36:3;0	PC	GPL	MEM	36	3	0
PC 36:4;0	PC	GPL	MEM	36	4	0
PC 36:5;0	PC	GPL	MEM	36	5	0
PE 26:0;0	PE	GPL	MEM	26	0	0
PE 28:0;0	PE	GPL	MEM	28	0	0
PE 28:1;0	PE	GPL	MEM	28	1	0
PE 30:0;0	PE	GPL	MEM	30	0	0
PE 30:1;0	PE	GPL	MEM	30	1	0
PE 30:2;0	PE	GPL	MEM	30	2	0
PE 31:0;0	PE	GPL	MEM	30	0	0
PE 31:1;0	PE	GPL	MEM	31	1	0
PE 32:0;0	PE	GPL	MEM	32	0	0
PE 32:1;0	PE	GPL	MEM	32	1	0
PE 32:2;0	PE	GPL	MEM	32	2	0
PE 32:3;0	PE	GPL	MEM	32	3	0
PE 33:0;0	PE	GPL	MEM	33	0	0
PE 33:1;0	PE	GPL	MEM	33	1	0
PE 33:2;0	PE	GPL	MEM	33	2	0
PE 34:1;0	PE	GPL	MEM	34	1	0
PE 34:2;0	PE	GPL	MEM	34	2	0
PE 34:3;0	PE	GPL	MEM	34	3	0
PE 34:4;0	PE	GPL	MEM	34	4	0
PE 35:1;0	PE	GPL	MEM	35	1	0
PE 35:2;0	PE	GPL	MEM	35	2	0
PE 35:3;0	PE	GPL	MEM	35	3	0
PE 36:1;0	PE	GPL	MEM	36	1	0
PE 36:2;0	PE	GPL	MEM	36	2	0
PE 36:3;0	PE	GPL	MEM	36	3	0
PE 36:4;0	PE	GPL	MEM	36	4	0
PE 36:5;0	PE	GPL	MEM	36	5	0
PE 36:6;0	PE	GPL	MEM	36	6	0
PE 37:1;0	PE	GPL	MEM	37	1	0
PE 37:2;0	PE	GPL	MEM	37	2	0
PE 37:3;0	PE	GPL	MEM	37	3	0
PE 38:1;0	PE	GPL	MEM	38	1	0
PE 38:2;0	PE	GPL	MEM	38	2	0
PE 38:3;0	PE	GPL	MEM	38	3	0
PG 30:1;0	PG	GPL	MEM	30	1	0
PG 30:2;0	PG	GPL	MEM	30	2	0
PG 32:0;0	PG	GPL	MEM	32	0	0
PG 32:1;0	PG	GPL	MEM	32	1	0
PG 32:2;0	PG	GPL	MEM	32	2	0
PG 32:3;0	PG	GPL	MEM	32	3	0
PG 34:0;0	PG	GPL	MEM	34	0	0
PG 34:1;0	PG	GPL	MEM	34	1	0
PG 34:2;0	PG	GPL	MEM	34	2	0
PG 34:3;0	PG	GPL	MEM	34	3	0
PG 35:3;0	PG	GPL	MEM	35	3	0
PG 36:2;0	PG	GPL	MEM	36	2	0
PG 36:3;0	PG	GPL	MEM	36	3	0
PG 36:4;0	PG	GPL	MEM	36	4	0
PI 30:0;0	PI	GPL	MEM	30	0	0
PI 30:1;0	PI	GPL	MEM	30	1	0
PI 30:2;0	PI	GPL	MEM	30	2	0
PI 31:0;0	PI	GPL	MEM	31	0	0
PI 31:1;0	PI	GPL	MEM	31	1	0
PI 32:0;0	PI	GPL	MEM	32	0	0
PI 32:1;0	PI	GPL	MEM	32	1	0
PI 32:2;0	PI	GPL	MEM	32	2	0
PI 33:0;0	PI	GPL	MEM	33	0	0
PI 33:1;0	PI	GPL	MEM	33	1	0
PI 33:2;0	PI	GPL	MEM	33	2	0
PI 34:0;0	PI	GPL	MEM	34	0	0
PI 34:1;0	PI	GPL	MEM	34	1	0
PI 34:2;0	PI	GPL	MEM	34	2	0
PI 34:3;0	PI	GPL	MEM	34	3	0
PI 34:4;0	PI	GPL	MEM	34	4	0
PI 35:1;0	PI	GPL	MEM	35	1	0
PI 35:2;0	PI	GPL	MEM	35	2	0
PI 35:3;0	PI	GPL	MEM	35	3	0
PI 36:1;0	PI	GPL	MEM	36	1	0
PI 36:2;0	PI	GPL	MEM	36	2	0
PI 36:3;0	PI	GPL	MEM	36	3	0
PI 36:4;0	PI	GPL	MEM	36	4	0
PI 36:5;0	PI	GPL	MEM	36	5	0
PI 36:6;0	PI	GPL	MEM	36	6	0
PS 32:1;0	PS	GPL	MEM	32	1	0
PS 32:2;0	PS	GPL	MEM	32	2	0
PS 32:3;0	PS	GPL	MEM	32	3	0
PS 33:1;0	PS	GPL	MEM	33	1	0
PS 34:0;0	PS	GPL	MEM	34	0	0
PS 34:1;0	PS	GPL	MEM	34	1	0
PS 34:2;0	PS	GPL	MEM	34	2	0
PS 34:3;0	PS	GPL	MEM	34	3	0
PS 34:4;0	PS	GPL	MEM	34	4	0
PS 35:1;0	PS	GPL	MEM	35	1	0
PS 35:2;0	PS	GPL	MEM	35	2	0
PS 36:0;0	PS	GPL	MEM	36	0	0
PS 36:1;0	PS	GPL	MEM	36	1	0
PS 36:2;0	PS	GPL	MEM	36	2	0
PS 36:3;0	PS	GPL	MEM	36	3	0
PS 36:4;0	PS	GPL	MEM	36	4	0
PS 36:5;0	PS	GPL	MEM	36	5	0
PS 36:6;0	PS	GPL	MEM	36	6	0
PS 37:2;0	PS	GPL	MEM	37	2	0
PS 38:0;0	PS	GPL	MEM	38	0	0
PS 38:1;0	PS	GPL	MEM	38	1	0
PS 38:2;0	PS	GPL	MEM	38	2	0
PS 38:3;0	PS	GPL	MEM	38	3	0
PS 38:4;0	PS	GPL	MEM	38	4	0
PS 38:7;0	PS	GPL	MEM	38	7	0
METABOLITES_END
#END