#METABOLOMICS WORKBENCH Chen_xy2022_20221227_001715 DATATRACK_ID:3673 STUDY_ID:ST002483 ANALYSIS_ID:AN004053 PROJECT_ID:PR001605 VERSION 1 CREATED_ON February 21, 2023, 7:38 am #PROJECT PR:PROJECT_TITLE Transcriptomics integrated with lipidomics reveals the effect of Acer truncatum PR:PROJECT_TITLE Bunge Seed Oil on neuroprotective by altitude ischemia-hypoxic encephalopathy PR:PROJECT_TITLE rats PR:PROJECT_SUMMARY Neonatal hypoxic-ischemic encephalopathy is a brain injury caused by perinatal PR:PROJECT_SUMMARY asphyxia and hypoxia, which can cause permanent neurological impairment in PR:PROJECT_SUMMARY severe cases. It is one of the common causes of neurological injury in children, PR:PROJECT_SUMMARY especially in the plateau area where medical services are scarce. Foreign PR:PROJECT_SUMMARY studies have shown that neuric acid is the core natural component of brain nerve PR:PROJECT_SUMMARY cells and nerve tissue. It is a special substance found in the world so far that PR:PROJECT_SUMMARY can promote the repair and regeneration of damaged nerve tissue. Transcriptomics PR:PROJECT_SUMMARY was used to analyze the protective mechanism and possible signaling pathway of PR:PROJECT_SUMMARY neuric acid against hypoxic-ischemic encephalopathy in newborns in plateau PR:PROJECT_SUMMARY region PR:INSTITUTE Bao Feng Key Laboratory of Genetics and Metabolism PR:LAST_NAME Chen PR:FIRST_NAME Xianyang PR:ADDRESS Room 525, Floor 5, Building 3, Yard 10, Anxiang Street, Airport Economic Core PR:ADDRESS Zone, Shunyi District, Beijing PR:EMAIL cxyibcas@163.com PR:PHONE 13681398052 #STUDY ST:STUDY_TITLE Brain metabonomics of rats in CO and ASO groups from 4500m were compared. ST:STUDY_SUMMARY To study the cerebral protective of nervonic acid after hypoxia-ischemia (HI), ST:STUDY_SUMMARY After 48 hours of successful modeling, we re-group the HIE group: CO group (n=8) ST:STUDY_SUMMARY and ASO group (n=8). The ASO group were treated by Acer truncatum Bunge seed oil ST:STUDY_SUMMARY for 30 days, rats in the CO group were treated by an equal volume of corn oil. ST:INSTITUTE Bao Feng Key Laboratory of Genetics and Metabolism ST:LAST_NAME Chen ST:FIRST_NAME Xianyang ST:ADDRESS Room 525, Floor 5, Building 3, Yard 10, Anxiang Street, Airport Economic Core ST:ADDRESS Zone, Shunyi District, Beijing ST:EMAIL cxyibcas@163.com ST:PHONE 13681398052 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Rattus norvegicus #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - 20220818_12 Group:H06 Pos or Neg=Pos; RAW_FILE_NAME=20220818_12.mzXML SUBJECT_SAMPLE_FACTORS - 20220818_13 Group:HS1 Pos or Neg=Pos; RAW_FILE_NAME=20220818_13.mzXML SUBJECT_SAMPLE_FACTORS - 20220818_14 Group:H04 Pos or Neg=Pos; RAW_FILE_NAME=20220818_14.mzXML SUBJECT_SAMPLE_FACTORS - 20220818_15 Group:HS3 Pos or Neg=Pos; RAW_FILE_NAME=20220818_15.mzXML SUBJECT_SAMPLE_FACTORS - 20220818_16 Group:H07 Pos or Neg=Pos; RAW_FILE_NAME=20220818_16.mzXML SUBJECT_SAMPLE_FACTORS - 20220818_17 Group:HS5 Pos or Neg=Pos; RAW_FILE_NAME=20220818_17.mzXML SUBJECT_SAMPLE_FACTORS - 20220818_18 Group:H05 Pos or Neg=Pos; RAW_FILE_NAME=20220818_18.mzXML SUBJECT_SAMPLE_FACTORS - 20220818_19 Group:HS4 Pos or Neg=Pos; RAW_FILE_NAME=20220818_19.mzXML SUBJECT_SAMPLE_FACTORS - 20220818_21 Group:H02 Pos or Neg=Pos; RAW_FILE_NAME=20220818_21.mzXML SUBJECT_SAMPLE_FACTORS - 20220818_22 Group:HS2 Pos or Neg=Pos; RAW_FILE_NAME=20220818_22.mzXML SUBJECT_SAMPLE_FACTORS - 20220818_23 Group:H03 Pos or Neg=Pos; RAW_FILE_NAME=20220818_23.mzXML SUBJECT_SAMPLE_FACTORS - 20220818_24 Group:HS8 Pos or Neg=Pos; RAW_FILE_NAME=20220818_24.mzXML SUBJECT_SAMPLE_FACTORS - 20220818_25 Group:H08 Pos or Neg=Pos; RAW_FILE_NAME=20220818_25.mzXML SUBJECT_SAMPLE_FACTORS - 20220818_26 Group:HS6 Pos or Neg=Pos; RAW_FILE_NAME=20220818_26.mzXML SUBJECT_SAMPLE_FACTORS - 20220818_27 Group:H01 Pos or Neg=Pos; RAW_FILE_NAME=20220818_27.mzXML SUBJECT_SAMPLE_FACTORS - 20220818_28 Group:HS7 Pos or Neg=Pos; RAW_FILE_NAME=20220818_28.mzXML SUBJECT_SAMPLE_FACTORS - 20220819_12 Group:H06 Pos or Neg=Neg; RAW_FILE_NAME=20220819_12.mzXML SUBJECT_SAMPLE_FACTORS - 20220819_13 Group:HS1 Pos or Neg=Neg; RAW_FILE_NAME=20220819_13.mzXML SUBJECT_SAMPLE_FACTORS - 20220819_14 Group:H04 Pos or Neg=Neg; RAW_FILE_NAME=20220819_14.mzXML SUBJECT_SAMPLE_FACTORS - 20220819_15 Group:HS3 Pos or Neg=Neg; RAW_FILE_NAME=20220819_15.mzXML SUBJECT_SAMPLE_FACTORS - 20220819_16 Group:H07 Pos or Neg=Neg; RAW_FILE_NAME=20220819_16.mzXML SUBJECT_SAMPLE_FACTORS - 20220819_17 Group:HS5 Pos or Neg=Neg; RAW_FILE_NAME=20220819_17.mzXML SUBJECT_SAMPLE_FACTORS - 20220819_18 Group:H05 Pos or Neg=Neg; RAW_FILE_NAME=20220819_18.mzXML SUBJECT_SAMPLE_FACTORS - 20220819_19 Group:HS4 Pos or Neg=Neg; RAW_FILE_NAME=20220819_19.mzXML SUBJECT_SAMPLE_FACTORS - 20220819_21 Group:H02 Pos or Neg=Neg; RAW_FILE_NAME=20220819_21.mzXML SUBJECT_SAMPLE_FACTORS - 20220819_22 Group:HS2 Pos or Neg=Neg; RAW_FILE_NAME=20220819_22.mzXML SUBJECT_SAMPLE_FACTORS - 20220819_23 Group:H03 Pos or Neg=Neg; RAW_FILE_NAME=20220819_23.mzXML SUBJECT_SAMPLE_FACTORS - 20220819_24 Group:HS8 Pos or Neg=Neg; RAW_FILE_NAME=20220819_24.mzXML SUBJECT_SAMPLE_FACTORS - 20220819_25 Group:H08 Pos or Neg=Neg; RAW_FILE_NAME=20220819_25.mzXML SUBJECT_SAMPLE_FACTORS - 20220819_26 Group:HS6 Pos or Neg=Neg; RAW_FILE_NAME=20220819_26.mzXML SUBJECT_SAMPLE_FACTORS - 20220819_27 Group:H01 Pos or Neg=Neg; RAW_FILE_NAME=20220819_27.mzXML SUBJECT_SAMPLE_FACTORS - 20220819_28 Group:HS7 Pos or Neg=Neg; RAW_FILE_NAME=20220819_28.mzXML #COLLECTION CO:COLLECTION_SUMMARY Take the whole brain according to the experimental purpose, and then immediately CO:COLLECTION_SUMMARY wash the blood residue on the tissue with precooled normal saline or deionized CO:COLLECTION_SUMMARY water. Divide the homogenized brain tissue into sterile tubes for every 250mg of CO:COLLECTION_SUMMARY tissue samples. Take multiple tubes for each sample for backup, mark them, seal CO:COLLECTION_SUMMARY them with sealing membrane, and freeze them at - 80 ℃. CO:SAMPLE_TYPE Brain CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY To study the cerebral protective of nervonic acid after hypoxia-ischemia (HI). TR:TREATMENT_SUMMARY After 48 hours of successful modeling, we re-group the HIE group: CO group (n=8) TR:TREATMENT_SUMMARY and ASO group (n=8). The ASO group were treated by Acer truncatum Bunge seed oil TR:TREATMENT_SUMMARY for 30 days, rats in the CO group were treated by an equal volume of corn oil. TR:TREATMENT_SUMMARY Based on the individual recommended intake NA of 0.3 g/kg/d, the equivalent dose TR:TREATMENT_SUMMARY of the drug was calculated according to the bodyweight of the human and the TR:TREATMENT_SUMMARY animal, in which the daily dose of the rat was equivalent to six times that of TR:TREATMENT_SUMMARY adults. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY We use isopropanol (IPA) to extract lipids from serum and brain tissue. In SP:SAMPLEPREP_SUMMARY brief, 100mg of brain tissue homogenized was extracted with 600 μL of precooled SP:SAMPLEPREP_SUMMARY IPA temperature of 4°C, vortexed for 1 minute and incubated for 10 minutes at SP:SAMPLEPREP_SUMMARY room temperature; the extracted mixture was stored overnight at -20 ° C. SP:SAMPLEPREP_SUMMARY Samples were centrifuged for 20 minutes at 14000 rpm, and the supernatants were SP:SAMPLEPREP_SUMMARY transferred into a new centrifuge tube and diluted to 1:10 with IPA/ACN/H2O SP:SAMPLEPREP_SUMMARY (2.5:1:1, v: v: v). Before LC/MS analysis, the sample was stored at -80°C. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Waters Acquity I-Class CH:COLUMN_NAME Waters Acquity BEH C8 (100 x 2.1mm, 1.7um) CH:SOLVENT_A 60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate CH:SOLVENT_B 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate CH:FLOW_GRADIENT The injected 1 µL sample was initially eluted with 40% B, graded linearly to CH:FLOW_GRADIENT 43% B in 2 minutes, and then increased to 50% B in 0.1 minutes. In the next 9.9 CH:FLOW_GRADIENT minutes, the gradient was further increased to 54% B and then increased to 70% CH:FLOW_GRADIENT in 0.1 minutes. In the last part of the gradient, the amount of B was increased CH:FLOW_GRADIENT to 99% in 5.9 minutes. Finally, the column was balanced for 1.9 minutes before CH:FLOW_GRADIENT the next injection, and solution B returned to 40% in 0.1 minutes. CH:FLOW_RATE 0.4 mL/min CH:COLUMN_TEMPERATURE 550 ℃ CH:METHODS_FILENAME Chrom__methods_.pdf #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Waters Xevo-G2-XS MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS - MS:MS_RESULTS_FILE ST002483_AN004053_Results.txt UNITS:Peak area Has m/z:Yes Has RT:Yes RT units:Minutes #END