#METABOLOMICS WORKBENCH ljx_20230310_001741 DATATRACK_ID:3783 STUDY_ID:ST002395 ANALYSIS_ID:AN004119 VERSION 1 CREATED_ON 03-20-2023 #PROJECT PR:PROJECT_TITLE A Mammalian Conserved Circular RNA CircLARP2 Regulates Hepatocellular Carcinoma PR:PROJECT_TITLE Metastasis and Lipid Metabolism PR:PROJECT_SUMMARY Circular RNAs (circRNAs) have emerged as crucial regulators in physiology and PR:PROJECT_SUMMARY human diseases. However, evolutionarily conserved circRNAs with potent functions PR:PROJECT_SUMMARY in cancers are rarely reported. Here, we identified a mammalian conserved PR:PROJECT_SUMMARY circRNA circLARP2 that played critical roles in hepatocellular carcinoma (HCC). PR:PROJECT_SUMMARY With clinical specimens, we found that patients with high circLARP2 levels in PR:PROJECT_SUMMARY HCC had advanced prognostic stage and poor overall survival. CircLARP2 PR:PROJECT_SUMMARY facilitated HCC metastasis and lipid accumulation in HCC cell lines. CircLARP2 PR:PROJECT_SUMMARY was one of the rare ones that were identified in HCC metastasis and conserved in PR:PROJECT_SUMMARY mammals, which enabled further studies with animal models. CircLARP2-deficient PR:PROJECT_SUMMARY mice exhibited reduced metastasis and less lipid accumulation in an induced HCC PR:PROJECT_SUMMARY model. We provided lines of evidence at molecular, cellular, and whole PR:PROJECT_SUMMARY organismal levels, to support that circLARP2 functioned as a protein sponge of PR:PROJECT_SUMMARY AUF1. CircLARP2 sequestered AUF1 from binding to LKB1 mRNA, which led to PR:PROJECT_SUMMARY decreased LKB1 mRNA stability and lower LKB1 protein levels. LKB1 as a kinase PR:PROJECT_SUMMARY promoted the phosphorylation of AMPK and then the phosphorylation of ACC, the PR:PROJECT_SUMMARY rate limiting enzyme of fatty acid synthesis. Knockdown of Lkb1 with AAV8-shLkb1 PR:PROJECT_SUMMARY in mice HCC model also proved that Lkb1 was a key element in the regulation. PR:PROJECT_SUMMARY Through this AUF1-LKB1-AMPK-ACC pathway, circLARP2 promoted HCC metastasis and PR:PROJECT_SUMMARY lipid accumulation. PR:INSTITUTE University of Science and Technology of China PR:LAST_NAME Li PR:FIRST_NAME Jingxin PR:ADDRESS 443 Huangshan Road PR:EMAIL ljx0418@mail.ustc.edu.cn PR:PHONE 00-86-0551-63600137 PR:DOI http://dx.doi.org/10.21228/M8CM5D #STUDY ST:STUDY_TITLE A Mammalian Conserved Circular RNA CircLARP2 Regulates Hepatocellular Carcinoma ST:STUDY_TITLE Metastasis and Lipid Metabolism (Part 2) ST:STUDY_SUMMARY Circular RNAs (circRNAs) have emerged as crucial regulators in physiology and ST:STUDY_SUMMARY human diseases. However, evolutionarily conserved circRNAs with potent functions ST:STUDY_SUMMARY in cancers are rarely reported. Here, we identified a mammalian conserved ST:STUDY_SUMMARY circRNA circLARP2 that played critical roles in hepatocellular carcinoma (HCC). ST:STUDY_SUMMARY With clinical specimens, we found that patients with high circLARP2 levels in ST:STUDY_SUMMARY HCC had advanced prognostic stage and poor overall survival. CircLARP2 ST:STUDY_SUMMARY facilitated HCC metastasis and lipid accumulation in HCC cell lines. CircLARP2 ST:STUDY_SUMMARY was one of the rare ones that were identified in HCC metastasis and conserved in ST:STUDY_SUMMARY mammals, which enabled further studies with animal models. CircLARP2-deficient ST:STUDY_SUMMARY mice exhibited reduced metastasis and less lipid accumulation in an induced HCC ST:STUDY_SUMMARY model. We provided lines of evidence at molecular, cellular, and whole ST:STUDY_SUMMARY organismal levels, to support that circLARP2 functioned as a protein sponge of ST:STUDY_SUMMARY AUF1. CircLARP2 sequestered AUF1 from binding to LKB1 mRNA, which led to ST:STUDY_SUMMARY decreased LKB1 mRNA stability and lower LKB1 protein levels. LKB1 as a kinase ST:STUDY_SUMMARY promoted the phosphorylation of AMPK and then the phosphorylation of ACC, the ST:STUDY_SUMMARY rate limiting enzyme of fatty acid synthesis. Knockdown of Lkb1 with AAV8-shLkb1 ST:STUDY_SUMMARY in mice HCC model also proved that Lkb1 was a key element in the regulation. ST:STUDY_SUMMARY Through this AUF1-LKB1-AMPK-ACC pathway, circLARP2 promoted HCC metastasis and ST:STUDY_SUMMARY lipid accumulation. ST:INSTITUTE University of Science and Technology of China ST:LAST_NAME Li ST:FIRST_NAME Jingxin ST:ADDRESS 443 Huangshan Road, HeFei, AnHui, 230022, China ST:EMAIL ljx0418@mail.ustc.edu.cn ST:PHONE 00-86-0551-63600137 ST:SUBMIT_DATE 2023-03-10 #SUBJECT SU:SUBJECT_TYPE Cultured cells SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - KO-1 Genotype:circLARP2-deficiency RAW_FILE_NAME=KO-1.mzXML SUBJECT_SAMPLE_FACTORS - KO-2 Genotype:circLARP2-deficiency RAW_FILE_NAME=KO-2.mzXML SUBJECT_SAMPLE_FACTORS - KO-3 Genotype:circLARP2-deficiency RAW_FILE_NAME=KO-3.mzXML SUBJECT_SAMPLE_FACTORS - WT-1 Genotype:Wild-type RAW_FILE_NAME=WT-1.mzXML SUBJECT_SAMPLE_FACTORS - WT-2 Genotype:Wild-type RAW_FILE_NAME=WT-2.mzXML SUBJECT_SAMPLE_FACTORS - WT-3 Genotype:Wild-type RAW_FILE_NAME=WT-3.mzXML #COLLECTION CO:COLLECTION_SUMMARY 500 μL cold extraction buffer (methanol: acetonitrile: water = 2:2:1, v/v/v) CO:COLLECTION_SUMMARY was added to each sample. Samples were sonicated for 2 minutes and centrifuged CO:COLLECTION_SUMMARY at 14,000 g for 5 min at 4 °C. Supernatants were thoroughly lyophilized CO:COLLECTION_SUMMARY (FreeZone 6 Liter, Labconco, USA) and reconstituted in 50 μL of methanol-water CO:COLLECTION_SUMMARY (1:1, v/v) just prior to measurement. CO:SAMPLE_TYPE Cultured cells #TREATMENT TR:TREATMENT_SUMMARY Twenty-four hours after seeding, cells were washed three times with 0.9% NaCl TR:TREATMENT_SUMMARY and incubated in DMEM without glucose, glutamine or sodium pyruvate supplemented TR:TREATMENT_SUMMARY with 15 mM [U-13C] glucose, 4 mM [U-13C] glutamine, 10% FBS and the TR:TREATMENT_SUMMARY indicated treatment condition for 24 h before metabolite extraction. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Supernatants were thoroughly lyophilized (FreeZone 6 Liter, Labconco, USA) and SP:SAMPLEPREP_SUMMARY reconstituted in 50 μL of methanol-water (1:1, v/v) just prior to measurement. #CHROMATOGRAPHY CH:INSTRUMENT_NAME Thermo Vanquish CH:COLUMN_NAME SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) CH:COLUMN_TEMPERATURE 45 CH:FLOW_GRADIENT The initial mobile phase was 30% solvent B at a flow rate of 300 μL/min. It was CH:FLOW_GRADIENT held for 2 min, and then linearly increased to 100% solvent B in 23 min, CH:FLOW_GRADIENT followed by equilibrating at 5% solvent B for 10 min. CH:FLOW_RATE 300 μL/min CH:SOLVENT_A acetonitrile–water (6:4, v/v) with 0.1% formic acid and 0.1mM ammonium formate CH:SOLVENT_B acetonitrile–isopropanol (1:9, v/v) with 0.1% formic acid and 0.1 mM ammonium CH:SOLVENT_B formate CH:CHROMATOGRAPHY_TYPE HILIC #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Q Exactive Plus Orbitrap MS:INSTRUMENT_TYPE Triple TOF MS:MS_TYPE ESI MS:MS_COMMENTS Data processing and ion annotation based on accurate mass were performed in MS:MS_COMMENTS TraceFinder 5.0 (Thermo Fisher) and Xcalibur 4.0 (Thermo Fisher). MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS peak area MS_METABOLITE_DATA_START Samples KO-1 KO-2 KO-3 WT-1 WT-2 WT-3 Factors Genotype:circLARP2-deficiency Genotype:circLARP2-deficiency Genotype:circLARP2-deficiency Genotype:Wild-type Genotype:Wild-type Genotype:Wild-type Arachidic acid m+0 65.8199 62.0970 66.5744 43.3174 46.3954 46.0486 Arachidic acid m+10 1.7447 2.9545 3.3207 3.3810 4.0294 3.5015 Arachidic acid m+12 5.9175 6.3429 5.8194 6.9783 6.4080 6.5889 Arachidic acid m+14 4.5316 3.8269 2.7695 8.1654 8.1786 8.3091 Arachidic acid m+16 2.9699 2.8846 3.2421 7.5937 6.7301 6.3663 Arachidic acid m+18 0.8834 1.2147 0.7688 4.0821 3.6477 3.7712 Arachidic acid m+2 11.4655 13.0192 12.9955 15.9532 13.9158 14.7085 Arachidic acid m+20 0.2139 0.7003 0.5431 Arachidic acid m+4 4.2366 4.3463 2.2295 6.8302 6.6682 6.4753 Arachidic acid m+6 0.2557 0.3079 0.0736 0.2354 0.2260 Arachidic acid m+8 1.1776 2.0451 1.0580 0.5822 1.2343 1.5511 Behenic acid m+0 72.7991 70.9511 72.5679 56.0235 57.8558 64.8660 Behenic acid m+10 1.8372 2.1694 2.1757 1.5921 1.6857 1.2580 Behenic acid m+12 2.8366 3.2413 2.9141 3.1265 2.9179 2.6569 Behenic acid m+14 3.2540 3.5469 3.0627 4.7083 4.6664 4.1219 Behenic acid m+16 2.3481 2.9471 2.4461 5.3185 4.9781 4.0990 Behenic acid m+18 1.3168 1.5926 1.2342 3.8497 3.4414 3.1029 Behenic acid m+2 6.0757 5.8685 5.4711 7.5152 7.3754 6.2757 Behenic acid m+20 0.3991 0.4002 0.4642 1.7919 1.7086 1.5443 Behenic acid m+4 5.5407 5.7415 5.5032 9.0081 8.5990 6.8428 Behenic acid m+6 1.7979 1.6870 1.8192 3.1103 3.1250 2.5733 Behenic acid m+8 0.9956 1.1486 1.0380 0.6050 0.6621 0.4115 Docosadienoic acid m+0 50.3717 51.3162 49.1392 38.8793 37.1196 43.5271 Docosadienoic acid m+10 0.0000 0.0000 Docosadienoic acid m+12 1.7091 0.8399 0.9313 1.3221 0.9308 Docosadienoic acid m+14 3.4237 1.6143 2.9345 Docosadienoic acid m+16 1.6043 2.1450 2.5687 Docosadienoic acid m+2 32.0852 32.1900 38.4310 30.8112 30.3589 29.3413 Docosadienoic acid m+4 15.8335 15.6536 12.4298 22.5744 26.1681 18.8874 Docosadienoic acid m+6 1.7219 1.2717 1.8100 Docosapentaenoic acid m+0 95.9877 96.4198 95.7934 92.8152 92.9969 92.8188 Docosapentaenoic acid m+2 4.0123 3.5802 4.2066 7.1724 7.0031 7.0893 Docosapentaenoic acid m+4 0.0124 0.0919 Docosatetraenoic acid m+0 89.4519 87.9756 89.5904 84.7439 85.6711 86.2535 Docosatetraenoic acid m+2 10.5481 12.0244 10.4096 14.0600 14.3289 13.7072 Docosatetraenoic acid m+4 0.0393 Eicosadienoic acid 71.4436 87.2583 71.4605 54.4599 60.1163 62.2680 Eicosadienoic acid m+2 28.5564 12.7417 27.7665 39.8768 37.7386 34.0270 Eicosadienoic acid m+4 3.1803 2.1451 3.7050 Eicosatrienoic acid m+0 96.0282 96.9076 98.9448 91.4184 92.5887 92.5911 Eicosatrienoic acid m+2 3.9718 3.0924 1.0552 7.5379 7.4113 6.1461 Eicosatrienoic acid m+4 0.5648 0.8252 Elaidic acid m+0 47.6735 77.5919 65.1585 29.9935 47.5856 39.4325 Elaidic acid m+10 6.2720 6.2173 6.9950 8.3009 6.7820 6.0331 Elaidic acid m+12 7.8263 4.6426 9.6330 9.8475 10.8726 10.9179 Elaidic acid m+14 6.0465 3.7098 2.2896 13.1404 8.5400 9.9087 Elaidic acid m+16 1.5618 0.2796 0.8469 7.0270 4.9242 6.4620 Elaidic acid m+18 0.9703 0.8393 0.8477 Elaidic acid m+2 7.5271 6.2594 14.3848 10.4586 12.4314 Erucic acid m+0 65.5746 65.2230 62.8606 60.8227 52.8513 63.3623 Erucic acid m+10 0.6315 0.6280 0.6920 0.3922 0.5672 0.5038 Erucic acid m+12 1.8811 2.4804 2.2788 1.3917 0.8220 0.9343 Erucic acid m+14 1.4509 1.0026 1.5415 1.4475 2.1346 0.8910 Erucic acid m+16 1.0421 0.5290 0.3044 1.1241 1.2841 0.8339 Erucic acid m+18 0.8627 Erucic acid m+2 15.7412 16.1679 16.8722 15.2625 20.1915 15.3309 Erucic acid m+20 0.1124 Erucic acid m+4 13.1158 13.5762 14.6922 16.2606 19.8858 15.7771 Erucic acid m+6 0.5629 0.3929 0.7583 3.1736 2.1785 1.3729 Gadoleic acid m+0 48.7170 55.8980 45.1380 37.8368 38.0743 40.0472 Gadoleic acid m+10 0.7519 0.7869 1.6034 1.1660 0.8067 0.8674 Gadoleic acid m+12 3.0733 3.2521 3.3900 3.5016 2.9673 3.1211 Gadoleic acid m+14 2.5287 2.2990 2.8314 4.3722 4.1261 3.7563 Gadoleic acid m+16 0.5682 1.0135 1.6278 3.3396 2.7269 3.2553 Gadoleic acid m+18 0.1079 0.4008 0.6410 1.8214 1.9058 1.4514 Gadoleic acid m+2 0.1390 0.2527 0.2563 0.7172 0.0497 0.1806 Gadoleic acid m+20 0.1171 0.1741 0.2440 Gadoleic acid m+8 0.0941 0.0744 0.0967 0.0366 Hexadecenoic acid 88.1443 87.0269 88.1733 75.9693 81.5469 78.1907 Hexadecenoic acid m+10 3.4771 3.5374 2.4253 6.5009 3.2678 6.4559 Hexadecenoic acid m+12 2.2124 3.1204 2.7196 7.5002 4.9381 6.4292 Hexadecenoic acid m+14 2.1235 1.0922 2.0430 4.1131 4.7119 3.4006 Hexadecenoic acid m+16 0.7182 0.5108 0.6723 Hexadecenoic acid m+6 1.0522 0.7832 1.2120 1.5617 0.8010 0.6728 Hexadecenoic acid m+8 2.9899 4.2129 3.4259 3.0378 4.2229 3.4815 Lignoceric acid m+0 56.0714 54.7364 56.5335 47.1869 42.8841 53.1485 Lignoceric acid m+10 1.8535 1.9834 1.9400 1.1042 1.2325 0.8864 Lignoceric acid m+12 3.1120 3.3015 3.0641 2.1459 2.4776 2.0353 Lignoceric acid m+14 4.2866 4.3071 3.9791 3.8219 4.0577 3.3120 Lignoceric acid m+15 0.6182 0.6980 0.6955 0.7980 0.9275 0.8184 Lignoceric acid m+16 4.1002 4.0834 4.0210 5.1115 5.3970 4.5453 Lignoceric acid m+18 3.0053 2.9503 2.9185 4.8138 5.2636 4.3938 Lignoceric acid m+2 7.5486 7.7162 7.2017 7.4392 8.0864 6.7117 Lignoceric acid m+20 1.3875 1.6318 1.5791 3.4197 3.6751 2.9042 Lignoceric acid m+22 0.3657 0.3439 0.4579 1.5449 1.5115 1.2332 Lignoceric acid m+4 7.8304 7.8380 7.5953 8.5802 8.9215 7.5846 Lignoceric acid m+6 5.5297 5.4533 5.2827 7.5255 8.0824 6.5205 Lignoceric acid m+8 2.1034 2.0787 2.1338 2.5903 2.9522 2.3913 Myristic acid m+0 58.1334 84.0504 80.0151 56.6436 61.5555 60.9646 Myristic acid m+10 22.4325 19.9838 18.5302 17.4540 17.0129 Myristic acid m+12 8.2633 10.6253 13.7874 Myristic acid m+14 7.2644 Myristic acid m+6 8.6938 4.5483 3.9026 5.9551 Myristic acid m+8 10.7370 11.3996 9.2969 6.4611 2.2777 Nervonic acid m+0 56.5531 56.2379 58.1945 47.6533 48.8495 48.7953 Nervonic acid m+10 0.8610 0.8149 0.6641 0.5883 0.4985 0.4288 Nervonic acid m+12 1.1598 1.5964 1.2080 1.0798 0.9908 1.1732 Nervonic acid m+14 1.7365 1.8387 1.7834 1.9122 1.6999 1.6363 Nervonic acid m+16 1.7184 1.7930 1.7997 2.3762 2.0581 2.1264 Nervonic acid m+18 1.1692 1.2862 1.1736 2.1285 2.0240 2.0064 Nervonic acid m+2 11.9589 11.3264 10.9085 10.7165 10.5521 10.8899 Nervonic acid m+20 0.5800 0.5328 0.5893 1.3967 1.3210 1.2842 Nervonic acid m+22 0.1111 0.1042 0.0692 0.5475 0.5818 0.5593 Nervonic acid m+4 13.9998 14.2589 13.3925 16.0741 15.7829 15.6569 Nervonic acid m+6 7.7044 7.9750 7.7208 11.3580 11.2636 11.0592 Nervonic acid m+8 1.3689 1.2267 1.1477 1.7476 1.7256 1.8142 Oleic acid m+0 82.0465 81.6604 81.3792 73.7074 75.3170 77.0943 Oleic acid m+10 2.6794 3.0485 3.0223 3.1051 2.7757 2.6187 Oleic acid m+12 2.8552 3.0510 3.0023 4.1761 3.9399 3.5684 Oleic acid m+14 2.0217 2.0133 2.0815 3.8133 3.6346 3.2222 Oleic acid m+16 0.8564 0.7887 0.8506 2.1195 2.0013 1.8493 Oleic acid m+18 0.1232 0.0681 0.0719 0.4605 0.5221 0.4403 Oleic acid m+2 4.7757 4.8925 5.2923 7.6498 7.4672 6.7156 Oleic acid m+4 0.3440 0.3583 0.3561 0.3498 0.2312 0.3462 Oleic acid m+6 0.8973 0.9824 0.9797 0.7524 0.6388 0.6900 Oleic acid m+8 1.8849 1.9931 2.0122 1.7403 1.5739 1.4408 Palmitic acid m+0 70.9766 63.9859 67.6461 58.5571 66.8111 59.1641 Palmitic acid m+10 7.6744 9.2083 8.3894 9.1134 7.8796 8.6670 Palmitic acid m+12 6.3616 7.3204 7.4249 10.5004 8.0840 10.3177 Palmitic acid m+14 2.9286 3.4646 3.0830 6.9670 5.5124 6.5833 Palmitic acid m+16 0.5471 0.6447 0.3886 1.8949 1.5959 2.0379 Palmitic acid m+2 0.0823 0.3836 0.1340 0.2869 0.1496 0.3804 Palmitic acid m+4 0.7638 1.3523 1.0300 0.8306 0.5410 0.7371 Palmitic acid m+6 3.1021 3.9155 3.0621 2.5246 1.9796 2.2787 Palmitic acid m+8 6.0987 7.3789 6.4637 5.5716 4.3848 5.5003 Stearic acid m+0 86.4611 82.2308 87.2806 72.5323 82.5727 71.4936 Stearic acid m+10 2.4761 3.1931 2.1780 3.7191 2.2027 3.6891 Stearic acid m+12 2.5763 3.2848 2.5746 4.9596 3.2830 5.2380 Stearic acid m+14 1.8674 2.3766 1.6905 4.6793 3.0563 4.8711 Stearic acid m+16 0.6335 0.9435 0.6694 2.7575 1.6659 2.7230 Stearic acid m+18 0.0795 0.0614 0.1033 0.7050 0.4507 0.6710 Stearic acid m+2 2.4265 3.4213 2.2837 5.2366 3.4549 5.5287 Stearic acid m+4 0.1883 0.3881 0.1883 0.3378 0.0591 0.3061 Stearic acid m+6 0.7118 0.9308 0.7484 0.8199 0.4515 0.7626 Stearic acid m+8 1.6159 2.2305 1.3734 1.7984 1.1808 1.9393 Tricosanoic acid m+0 98.6126 99.4333 100.0000 99.7381 100.0000 100.0000 Tricosanoic acid m+2 1.3874 0.5667 0.2619 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant Arachidic acid m+0 8.42 311.29554999999999 Arachidic acid m+10 321.32905000000011 Arachidic acid m+12 323.33575000000013 Arachidic acid m+14 325.34245000000016 Arachidic acid m+16 327.34915000000018 Arachidic acid m+18 329.3558500000002 Arachidic acid m+2 313.30225000000002 Arachidic acid m+20 331.36255000000023 Arachidic acid m+4 315.30895000000004 Arachidic acid m+6 317.31565000000006 Arachidic acid m+8 319.32235000000009 Behenic acid m+0 9.1999999999999993 339.32684999999998 Behenic acid m+10 349.3603500000001 Behenic acid m+12 351.36705000000012 Behenic acid m+14 353.37375000000014 Behenic acid m+16 355.38045000000017 Behenic acid m+18 357.38715000000019 Behenic acid m+2 341.33355 Behenic acid m+20 359.39385000000021 Behenic acid m+4 343.34025000000003 Behenic acid m+6 345.34695000000005 Behenic acid m+8 347.35365000000007 Docosadienoic acid m+0 7.85 335.29554999999999 Docosadienoic acid m+10 345.32905000000011 Docosadienoic acid m+12 347.33575000000013 Docosadienoic acid m+14 349.34245000000016 Docosadienoic acid m+16 351.34915000000018 Docosadienoic acid m+2 337.30225000000002 Docosadienoic acid m+4 339.30895000000004 Docosadienoic acid m+6 341.31565000000006 Docosapentaenoic acid m+0 6.52 329.24860000000001 Docosapentaenoic acid m+2 331.25530000000003 Docosapentaenoic acid m+4 333.26200000000006 Docosatetraenoic acid m+0 6.83 331.26425 Docosatetraenoic acid m+2 333.27095000000003 Docosatetraenoic acid m+4 335.27765000000005 Eicosadienoic acid 7.13 307.26425 Eicosadienoic acid m+2 309.27095000000003 Eicosadienoic acid m+4 311.27765000000005 Eicosatrienoic acid m+0 6.59 305.24860000000001 Eicosatrienoic acid m+2 307.25530000000003 Eicosatrienoic acid m+4 309.26200000000006 Elaidic acid m+0 7.11 281.24860000000001 Elaidic acid m+10 291.28210000000013 Elaidic acid m+12 293.28880000000015 Elaidic acid m+14 295.29550000000017 Elaidic acid m+16 297.3022000000002 Elaidic acid m+18 299.30890000000022 Elaidic acid m+2 283.25530000000003 Erucic acid m+0 8.4 337.31119999999999 Erucic acid m+10 347.34670000000017 Erucic acid m+12 349.35380000000021 Erucic acid m+14 351.36090000000024 Erucic acid m+16 353.36800000000028 Erucic acid m+18 355.37510000000032 Erucic acid m+2 339.31830000000002 Erucic acid m+20 357.38220000000035 Erucic acid m+4 341.32540000000006 Erucic acid m+6 343.3325000000001 Gadoleic acid m+0 7.74 309.2799 Gadoleic acid m+10 319.31340000000012 Gadoleic acid m+12 321.32010000000014 Gadoleic acid m+14 323.32680000000016 Gadoleic acid m+16 325.33350000000019 Gadoleic acid m+18 327.34020000000021 Gadoleic acid m+2 311.28660000000002 Gadoleic acid m+20 329.34690000000023 Gadoleic acid m+8 317.30670000000009 Hexadecenoic acid 6.14 253.21729999999999 Hexadecenoic acid m+10 263.25082000000009 Hexadecenoic acid m+12 265.25752000000011 Hexadecenoic acid m+14 267.26422000000014 Hexadecenoic acid m+16 269.27092000000016 Hexadecenoic acid m+6 259.23742000000004 Hexadecenoic acid m+8 261.24412000000007 Lignoceric acid m+0 9.6 367.35814999999997 Lignoceric acid m+10 377.39165000000008 Lignoceric acid m+12 379.39835000000011 Lignoceric acid m+14 381.40505000000013 Lignoceric acid m+15 382.40840000000014 Lignoceric acid m+16 383.41175000000015 Lignoceric acid m+18 385.41845000000018 Lignoceric acid m+2 369.36484999999999 Lignoceric acid m+20 387.4251500000002 Lignoceric acid m+22 389.43185000000022 Lignoceric acid m+4 371.37155000000001 Lignoceric acid m+6 373.37825000000004 Lignoceric acid m+8 375.38495000000006 Myristic acid m+0 6 227.20165 Myristic acid m+10 237.23515000000012 Myristic acid m+12 239.24185000000014 Myristic acid m+14 241.24855000000017 Myristic acid m+6 233.22175000000007 Myristic acid m+8 235.22845000000009 Nervonic acid m+0 8.98 365.34249999999997 Nervonic acid m+10 375.37600000000009 Nervonic acid m+12 377.38270000000011 Nervonic acid m+14 379.38940000000014 Nervonic acid m+16 381.39610000000016 Nervonic acid m+18 383.40280000000018 Nervonic acid m+2 367.3492 Nervonic acid m+20 385.40950000000021 Nervonic acid m+22 387.41620000000023 Nervonic acid m+4 369.35590000000002 Nervonic acid m+6 371.36260000000004 Nervonic acid m+8 373.36930000000007 Oleic acid m+0 6.98 281.24860000000001 Oleic acid m+10 291.28210000000013 Oleic acid m+12 293.28880000000015 Oleic acid m+14 295.29550000000017 Oleic acid m+16 297.3022000000002 Oleic acid m+18 299.30890000000022 Oleic acid m+2 283.25530000000003 Oleic acid m+4 285.26200000000006 Oleic acid m+6 287.26870000000008 Oleic acid m+8 289.2754000000001 Palmitic acid m+0 6.89 255.23294999999999 Palmitic acid m+10 265.26645000000008 Palmitic acid m+12 267.2731500000001 Palmitic acid m+14 269.27985000000012 Palmitic acid m+16 271.28655000000015 Palmitic acid m+2 257.23964999999998 Palmitic acid m+4 259.24635000000001 Palmitic acid m+6 261.25305000000003 Palmitic acid m+8 263.25975000000005 Stearic acid m+0 7.72 283.26425 Stearic acid m+10 293.29775000000012 Stearic acid m+12 295.30445000000014 Stearic acid m+14 297.31115000000017 Stearic acid m+16 299.31785000000019 Stearic acid m+18 301.32455000000022 Stearic acid m+2 285.27095000000003 Stearic acid m+4 287.27765000000005 Stearic acid m+6 289.28435000000007 Stearic acid m+8 291.2910500000001 Tricosanoic acid m+0 9.32 353.34249999999997 Tricosanoic acid m+2 355.3492 METABOLITES_END #END