#METABOLOMICS WORKBENCH ssah9_20230310_161743 DATATRACK_ID:3785 STUDY_ID:ST002521 ANALYSIS_ID:AN004154 PROJECT_ID:PR001623
VERSION             	1
CREATED_ON             	March 16, 2023, 3:14 pm
#PROJECT
PR:PROJECT_TITLE                 	Wide-Coverage Serum Metabolomic Profiling Reveals a Comprehensive Lipidome
PR:PROJECT_TITLE                 	Signature of Ovarian Cancer.
PR:PROJECT_SUMMARY               	Distinguishing ovarian cancer (OC) from other benign or cancerous gynecological
PR:PROJECT_SUMMARY               	malignancies remains a critical unmet medical need with significant implications
PR:PROJECT_SUMMARY               	on patient survival. Substantially better results are observed when women with
PR:PROJECT_SUMMARY               	OC are correctly diagnosed and ensured the right treatment. However,
PR:PROJECT_SUMMARY               	non-specific symptoms along with our lack of understanding of OC pathogenesis
PR:PROJECT_SUMMARY               	hinder its diagnosis, consequently leading to a very low survival rate.
PR:PROJECT_SUMMARY               	Accumulating evidence suggests the link between OC and deregulated lipid
PR:PROJECT_SUMMARY               	metabolism. Most studies, however, are limited by small sample sizes and
PR:PROJECT_SUMMARY               	metabolite coverage, thereby constraining the robustness of the results. Here,
PR:PROJECT_SUMMARY               	we performed a comprehensive serum lipidome profiling of OC and various other
PR:PROJECT_SUMMARY               	gynecological malignancies (non-OC). A relatively large patient cohort with 208
PR:PROJECT_SUMMARY               	OC and 137 non-OC patients, including 93 OC patients with early-stage OC, was
PR:PROJECT_SUMMARY               	recruited from two independent clinical sites in South Korea. Samples were
PR:PROJECT_SUMMARY               	analyzed with high-coverage liquid chromatography high-resolution mass
PR:PROJECT_SUMMARY               	spectrometry, providing extensive lipidome coverage with 994 successfully
PR:PROJECT_SUMMARY               	annotated lipid features. Lipidome differences between OC and other
PR:PROJECT_SUMMARY               	gynecological malignancies were investigated via statistical and machine
PR:PROJECT_SUMMARY               	learning approaches. Our data suggest that lipidome alterations unique to OC can
PR:PROJECT_SUMMARY               	be detected as early as when the cancer is localized, and those changes amplify
PR:PROJECT_SUMMARY               	as the diseases progresses. Comparison of the relative lipid abundances revealed
PR:PROJECT_SUMMARY               	specific patterns based on lipid class with most lipid classes showing decreased
PR:PROJECT_SUMMARY               	abundance in ovarian cancer. This study provides a systemic analysis of lipidome
PR:PROJECT_SUMMARY               	alterations in OC, emphasizing the potential of circulating lipids as a
PR:PROJECT_SUMMARY               	complementary class of blood-based biomarkers for OC diagnosis.
PR:INSTITUTE                     	Georgia Institute of Technology
PR:LAST_NAME                     	Sah
PR:FIRST_NAME                    	Samyukta
PR:ADDRESS                       	901 Atlantic Dr NW, Atlanta, GA 30318
PR:EMAIL                         	ssah9@gatech.edu
PR:PHONE                         	574-678-0124
#STUDY
ST:STUDY_TITLE                   	Wide-Coverage Serum Metabolomic Profiling Reveals a Comprehensive Lipidome
ST:STUDY_TITLE                   	Signature of Ovarian Cancer.
ST:STUDY_SUMMARY                 	Distinguishing ovarian cancer (OC) from other benign or cancerous gynecological
ST:STUDY_SUMMARY                 	malignancies remains a critical unmet medical need with significant implications
ST:STUDY_SUMMARY                 	on patient survival. Substantially better results are observed when women with
ST:STUDY_SUMMARY                 	OC are correctly diagnosed and ensured the right treatment. However,
ST:STUDY_SUMMARY                 	non-specific symptoms along with our lack of understanding of OC pathogenesis
ST:STUDY_SUMMARY                 	hinder its diagnosis, consequently leading to a very low survival rate.
ST:STUDY_SUMMARY                 	Accumulating evidence suggests the link between OC and deregulated lipid
ST:STUDY_SUMMARY                 	metabolism. Most studies, however, are limited by small sample sizes and
ST:STUDY_SUMMARY                 	metabolite coverage, thereby constraining the robustness of the results. Here,
ST:STUDY_SUMMARY                 	we performed a comprehensive serum lipidome profiling of OC and various other
ST:STUDY_SUMMARY                 	gynecological malignancies (non-OC). A relatively large patient cohort with 208
ST:STUDY_SUMMARY                 	OC and 137 non-OC patients, including 93 OC patients with early-stage OC, was
ST:STUDY_SUMMARY                 	recruited from two independent clinical sites in South Korea. Samples were
ST:STUDY_SUMMARY                 	analyzed with high-coverage liquid chromatography high-resolution mass
ST:STUDY_SUMMARY                 	spectrometry, providing extensive lipidome coverage with 994 successfully
ST:STUDY_SUMMARY                 	annotated lipid features. Lipidome differences between OC and other
ST:STUDY_SUMMARY                 	gynecological malignancies were investigated via statistical and machine
ST:STUDY_SUMMARY                 	learning approaches. Our data suggest that lipidome alterations unique to OC can
ST:STUDY_SUMMARY                 	be detected as early as when the cancer is localized, and those changes amplify
ST:STUDY_SUMMARY                 	as the diseases progresses. Comparison of the relative lipid abundances revealed
ST:STUDY_SUMMARY                 	specific patterns based on lipid class with most lipid classes showing decreased
ST:STUDY_SUMMARY                 	abundance in ovarian cancer. This study provides a systemic analysis of lipidome
ST:STUDY_SUMMARY                 	alterations in OC, emphasizing the potential of circulating lipids as a
ST:STUDY_SUMMARY                 	complementary class of blood-based biomarkers for OC diagnosis.
ST:INSTITUTE                     	Georgia Institute of Technology
ST:LAST_NAME                     	Sah
ST:FIRST_NAME                    	Samyukta
ST:ADDRESS                       	901 Atlantic Dr NW, Atlanta, GA 30318
ST:EMAIL                         	ssah9@gatech.edu
ST:PHONE                         	574-678-0124
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	BOT1	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT1
SUBJECT_SAMPLE_FACTORS           	-	BOT2	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT2
SUBJECT_SAMPLE_FACTORS           	-	BOT3	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT3
SUBJECT_SAMPLE_FACTORS           	-	BOT4	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT4
SUBJECT_SAMPLE_FACTORS           	-	BOT5	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT5
SUBJECT_SAMPLE_FACTORS           	-	BOT6	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT6
SUBJECT_SAMPLE_FACTORS           	-	BOT7	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT7
SUBJECT_SAMPLE_FACTORS           	-	BOT8	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT8
SUBJECT_SAMPLE_FACTORS           	-	BOT9	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT9
SUBJECT_SAMPLE_FACTORS           	-	BOT10	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT10
SUBJECT_SAMPLE_FACTORS           	-	BOT11	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT11
SUBJECT_SAMPLE_FACTORS           	-	BOT12	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT12
SUBJECT_SAMPLE_FACTORS           	-	BOT13	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT13
SUBJECT_SAMPLE_FACTORS           	-	BOT14	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT14
SUBJECT_SAMPLE_FACTORS           	-	BOT15	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT15
SUBJECT_SAMPLE_FACTORS           	-	BOT16	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT16
SUBJECT_SAMPLE_FACTORS           	-	BOT17	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT17
SUBJECT_SAMPLE_FACTORS           	-	BOT18	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT18
SUBJECT_SAMPLE_FACTORS           	-	BOT19	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT19
SUBJECT_SAMPLE_FACTORS           	-	BOT20	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT20
SUBJECT_SAMPLE_FACTORS           	-	BOT21	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT21
SUBJECT_SAMPLE_FACTORS           	-	BOT22	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT22
SUBJECT_SAMPLE_FACTORS           	-	BOT23	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT23
SUBJECT_SAMPLE_FACTORS           	-	BOT24	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT24
SUBJECT_SAMPLE_FACTORS           	-	BOT25	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT25
SUBJECT_SAMPLE_FACTORS           	-	BOT26	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT26
SUBJECT_SAMPLE_FACTORS           	-	BOT27	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT27
SUBJECT_SAMPLE_FACTORS           	-	BOT28	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT28
SUBJECT_SAMPLE_FACTORS           	-	BOT29	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT29
SUBJECT_SAMPLE_FACTORS           	-	BOT30	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT30
SUBJECT_SAMPLE_FACTORS           	-	BOT31	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT31
SUBJECT_SAMPLE_FACTORS           	-	BOT32	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT32
SUBJECT_SAMPLE_FACTORS           	-	BOT33	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT33
SUBJECT_SAMPLE_FACTORS           	-	BOT34	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT34
SUBJECT_SAMPLE_FACTORS           	-	BOT35	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT35
SUBJECT_SAMPLE_FACTORS           	-	BOT36	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT36
SUBJECT_SAMPLE_FACTORS           	-	BOT37	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT37
SUBJECT_SAMPLE_FACTORS           	-	BOT38	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT38
SUBJECT_SAMPLE_FACTORS           	-	BOT39	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT39
SUBJECT_SAMPLE_FACTORS           	-	BOT40	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT40
SUBJECT_SAMPLE_FACTORS           	-	BOT41	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT41
SUBJECT_SAMPLE_FACTORS           	-	BOT42	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT42
SUBJECT_SAMPLE_FACTORS           	-	BOT43	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT43
SUBJECT_SAMPLE_FACTORS           	-	BOT44	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT44
SUBJECT_SAMPLE_FACTORS           	-	BOT45	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT45
SUBJECT_SAMPLE_FACTORS           	-	BOT46	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT46
SUBJECT_SAMPLE_FACTORS           	-	BOT47	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT47
SUBJECT_SAMPLE_FACTORS           	-	BOT48	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT48
SUBJECT_SAMPLE_FACTORS           	-	BOT49	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT49
SUBJECT_SAMPLE_FACTORS           	-	BOT50	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT50
SUBJECT_SAMPLE_FACTORS           	-	BOT51	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT51
SUBJECT_SAMPLE_FACTORS           	-	BOT52	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT52
SUBJECT_SAMPLE_FACTORS           	-	BOT53	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT53
SUBJECT_SAMPLE_FACTORS           	-	BOT54	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT54
SUBJECT_SAMPLE_FACTORS           	-	BOT55	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT55
SUBJECT_SAMPLE_FACTORS           	-	BOT56	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT56
SUBJECT_SAMPLE_FACTORS           	-	BOT57	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT57
SUBJECT_SAMPLE_FACTORS           	-	BOT58	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT58
SUBJECT_SAMPLE_FACTORS           	-	BOT59	Treatment:Benign Ovarian Tumor	RAW_FILE_NAME=BOT59
SUBJECT_SAMPLE_FACTORS           	-	BUT1	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT1
SUBJECT_SAMPLE_FACTORS           	-	BUT2	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT2
SUBJECT_SAMPLE_FACTORS           	-	BUT3	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT3
SUBJECT_SAMPLE_FACTORS           	-	BUT4	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT4
SUBJECT_SAMPLE_FACTORS           	-	BUT5	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT5
SUBJECT_SAMPLE_FACTORS           	-	BUT6	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT6
SUBJECT_SAMPLE_FACTORS           	-	BUT7	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT7
SUBJECT_SAMPLE_FACTORS           	-	BUT8	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT8
SUBJECT_SAMPLE_FACTORS           	-	BUT9	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT9
SUBJECT_SAMPLE_FACTORS           	-	BUT10	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT10
SUBJECT_SAMPLE_FACTORS           	-	BUT11	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT11
SUBJECT_SAMPLE_FACTORS           	-	BUT12	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT12
SUBJECT_SAMPLE_FACTORS           	-	BUT13	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT13
SUBJECT_SAMPLE_FACTORS           	-	BUT14	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT14
SUBJECT_SAMPLE_FACTORS           	-	BUT15	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT15
SUBJECT_SAMPLE_FACTORS           	-	BUT16	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT16
SUBJECT_SAMPLE_FACTORS           	-	BUT17	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT17
SUBJECT_SAMPLE_FACTORS           	-	BUT18	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT18
SUBJECT_SAMPLE_FACTORS           	-	BUT19	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT19
SUBJECT_SAMPLE_FACTORS           	-	BUT20	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT20
SUBJECT_SAMPLE_FACTORS           	-	BUT21	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT21
SUBJECT_SAMPLE_FACTORS           	-	BUT22	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT22
SUBJECT_SAMPLE_FACTORS           	-	BUT23	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT23
SUBJECT_SAMPLE_FACTORS           	-	BUT24	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT24
SUBJECT_SAMPLE_FACTORS           	-	BUT25	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT25
SUBJECT_SAMPLE_FACTORS           	-	BUT26	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT26
SUBJECT_SAMPLE_FACTORS           	-	BUT27	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT27
SUBJECT_SAMPLE_FACTORS           	-	BUT28	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT28
SUBJECT_SAMPLE_FACTORS           	-	BUT29	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT29
SUBJECT_SAMPLE_FACTORS           	-	BUT30	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT30
SUBJECT_SAMPLE_FACTORS           	-	BUT31	Treatment:benign uterine tumor	RAW_FILE_NAME=BUT31
SUBJECT_SAMPLE_FACTORS           	-	CC1	Treatment:Cervical cancer	RAW_FILE_NAME=CC1
SUBJECT_SAMPLE_FACTORS           	-	CC2	Treatment:Cervical cancer	RAW_FILE_NAME=CC2
SUBJECT_SAMPLE_FACTORS           	-	CC3	Treatment:Cervical cancer	RAW_FILE_NAME=CC3
SUBJECT_SAMPLE_FACTORS           	-	CC4	Treatment:Cervical cancer	RAW_FILE_NAME=CC4
SUBJECT_SAMPLE_FACTORS           	-	CC5	Treatment:Cervical cancer	RAW_FILE_NAME=CC5
SUBJECT_SAMPLE_FACTORS           	-	CC6	Treatment:Cervical cancer	RAW_FILE_NAME=CC6
SUBJECT_SAMPLE_FACTORS           	-	CC7	Treatment:Cervical cancer	RAW_FILE_NAME=CC7
SUBJECT_SAMPLE_FACTORS           	-	CC8	Treatment:Cervical cancer	RAW_FILE_NAME=CC8
SUBJECT_SAMPLE_FACTORS           	-	CC9	Treatment:Cervical cancer	RAW_FILE_NAME=CC9
SUBJECT_SAMPLE_FACTORS           	-	CC10	Treatment:Cervical cancer	RAW_FILE_NAME=CC10
SUBJECT_SAMPLE_FACTORS           	-	CC11	Treatment:Cervical cancer	RAW_FILE_NAME=CC11
SUBJECT_SAMPLE_FACTORS           	-	CC12	Treatment:Cervical cancer	RAW_FILE_NAME=CC12
SUBJECT_SAMPLE_FACTORS           	-	CC13	Treatment:Cervical cancer	RAW_FILE_NAME=CC13
SUBJECT_SAMPLE_FACTORS           	-	CC14	Treatment:Cervical cancer	RAW_FILE_NAME=CC14
SUBJECT_SAMPLE_FACTORS           	-	CC15	Treatment:Cervical cancer	RAW_FILE_NAME=CC15
SUBJECT_SAMPLE_FACTORS           	-	CC16	Treatment:Cervical cancer	RAW_FILE_NAME=CC16
SUBJECT_SAMPLE_FACTORS           	-	CC17	Treatment:Cervical cancer	RAW_FILE_NAME=CC17
SUBJECT_SAMPLE_FACTORS           	-	CC18	Treatment:Cervical cancer	RAW_FILE_NAME=CC18
SUBJECT_SAMPLE_FACTORS           	-	CC19	Treatment:Cervical cancer	RAW_FILE_NAME=CC19
SUBJECT_SAMPLE_FACTORS           	-	CC20	Treatment:Cervical cancer	RAW_FILE_NAME=CC20
SUBJECT_SAMPLE_FACTORS           	-	CC21	Treatment:Cervical cancer	RAW_FILE_NAME=CC21
SUBJECT_SAMPLE_FACTORS           	-	CC22	Treatment:Cervical cancer	RAW_FILE_NAME=CC22
SUBJECT_SAMPLE_FACTORS           	-	CC23	Treatment:Cervical cancer	RAW_FILE_NAME=CC23
SUBJECT_SAMPLE_FACTORS           	-	CC24	Treatment:Cervical cancer	RAW_FILE_NAME=CC24
SUBJECT_SAMPLE_FACTORS           	-	CC25	Treatment:Cervical cancer	RAW_FILE_NAME=CC25
SUBJECT_SAMPLE_FACTORS           	-	CC26	Treatment:Cervical cancer	RAW_FILE_NAME=CC26
SUBJECT_SAMPLE_FACTORS           	-	CC27	Treatment:Cervical cancer	RAW_FILE_NAME=CC27
SUBJECT_SAMPLE_FACTORS           	-	CC28	Treatment:Cervical cancer	RAW_FILE_NAME=CC28
SUBJECT_SAMPLE_FACTORS           	-	CC29	Treatment:Cervical cancer	RAW_FILE_NAME=CC29
SUBJECT_SAMPLE_FACTORS           	-	CC30	Treatment:Cervical cancer	RAW_FILE_NAME=CC30
SUBJECT_SAMPLE_FACTORS           	-	CC31	Treatment:Cervical cancer	RAW_FILE_NAME=CC31
SUBJECT_SAMPLE_FACTORS           	-	CC32	Treatment:Cervical cancer	RAW_FILE_NAME=CC32
SUBJECT_SAMPLE_FACTORS           	-	CC33	Treatment:Cervical cancer	RAW_FILE_NAME=CC33
SUBJECT_SAMPLE_FACTORS           	-	CC34	Treatment:Cervical cancer	RAW_FILE_NAME=CC34
SUBJECT_SAMPLE_FACTORS           	-	CC35	Treatment:Cervical cancer	RAW_FILE_NAME=CC35
SUBJECT_SAMPLE_FACTORS           	-	CC36	Treatment:Cervical cancer	RAW_FILE_NAME=CC36
SUBJECT_SAMPLE_FACTORS           	-	CC37	Treatment:Cervical cancer	RAW_FILE_NAME=CC37
SUBJECT_SAMPLE_FACTORS           	-	CC38	Treatment:Cervical cancer	RAW_FILE_NAME=CC38
SUBJECT_SAMPLE_FACTORS           	-	CC39	Treatment:Cervical cancer	RAW_FILE_NAME=CC39
SUBJECT_SAMPLE_FACTORS           	-	CC40	Treatment:Cervical cancer	RAW_FILE_NAME=CC40
SUBJECT_SAMPLE_FACTORS           	-	CC41	Treatment:Cervical cancer	RAW_FILE_NAME=CC41
SUBJECT_SAMPLE_FACTORS           	-	CC42	Treatment:Cervical cancer	RAW_FILE_NAME=CC42
SUBJECT_SAMPLE_FACTORS           	-	CC43	Treatment:Cervical cancer	RAW_FILE_NAME=CC43
SUBJECT_SAMPLE_FACTORS           	-	CC44	Treatment:Cervical cancer	RAW_FILE_NAME=CC44
SUBJECT_SAMPLE_FACTORS           	-	CC45	Treatment:Cervical cancer	RAW_FILE_NAME=CC45
SUBJECT_SAMPLE_FACTORS           	-	CC46	Treatment:Cervical cancer	RAW_FILE_NAME=CC46
SUBJECT_SAMPLE_FACTORS           	-	CC47	Treatment:Cervical cancer	RAW_FILE_NAME=CC47
SUBJECT_SAMPLE_FACTORS           	-	CC48	Treatment:Cervical cancer	RAW_FILE_NAME=CC48
SUBJECT_SAMPLE_FACTORS           	-	CC49	Treatment:Cervical cancer	RAW_FILE_NAME=CC49
SUBJECT_SAMPLE_FACTORS           	-	CC50	Treatment:Cervical cancer	RAW_FILE_NAME=CC50
SUBJECT_SAMPLE_FACTORS           	-	CC51	Treatment:Cervical cancer	RAW_FILE_NAME=CC51
SUBJECT_SAMPLE_FACTORS           	-	CC52	Treatment:Cervical cancer	RAW_FILE_NAME=CC52
SUBJECT_SAMPLE_FACTORS           	-	CC53	Treatment:Cervical cancer	RAW_FILE_NAME=CC53
SUBJECT_SAMPLE_FACTORS           	-	CC54	Treatment:Cervical cancer	RAW_FILE_NAME=CC54
SUBJECT_SAMPLE_FACTORS           	-	CC55	Treatment:Cervical cancer	RAW_FILE_NAME=CC55
SUBJECT_SAMPLE_FACTORS           	-	CC56	Treatment:Cervical cancer	RAW_FILE_NAME=CC56
SUBJECT_SAMPLE_FACTORS           	-	CC57	Treatment:Cervical cancer	RAW_FILE_NAME=CC57
SUBJECT_SAMPLE_FACTORS           	-	CC58	Treatment:Cervical cancer	RAW_FILE_NAME=CC58
SUBJECT_SAMPLE_FACTORS           	-	CC59	Treatment:Cervical cancer	RAW_FILE_NAME=CC59
SUBJECT_SAMPLE_FACTORS           	-	CC60	Treatment:Cervical cancer	RAW_FILE_NAME=CC60
SUBJECT_SAMPLE_FACTORS           	-	OC1	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC1
SUBJECT_SAMPLE_FACTORS           	-	OC2	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC2
SUBJECT_SAMPLE_FACTORS           	-	OC3	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC3
SUBJECT_SAMPLE_FACTORS           	-	OC4	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC4
SUBJECT_SAMPLE_FACTORS           	-	OC5	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC5
SUBJECT_SAMPLE_FACTORS           	-	OC6	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC6
SUBJECT_SAMPLE_FACTORS           	-	OC7	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC7
SUBJECT_SAMPLE_FACTORS           	-	OC8	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC8
SUBJECT_SAMPLE_FACTORS           	-	OC9	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC9
SUBJECT_SAMPLE_FACTORS           	-	OC10	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC10
SUBJECT_SAMPLE_FACTORS           	-	OC11	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC11
SUBJECT_SAMPLE_FACTORS           	-	OC12	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC12
SUBJECT_SAMPLE_FACTORS           	-	OC13	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC13
SUBJECT_SAMPLE_FACTORS           	-	OC14	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC14
SUBJECT_SAMPLE_FACTORS           	-	OC15	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC15
SUBJECT_SAMPLE_FACTORS           	-	OC16	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC16
SUBJECT_SAMPLE_FACTORS           	-	OC17	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC17
SUBJECT_SAMPLE_FACTORS           	-	OC18	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC18
SUBJECT_SAMPLE_FACTORS           	-	OC19	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC19
SUBJECT_SAMPLE_FACTORS           	-	OC20	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC20
SUBJECT_SAMPLE_FACTORS           	-	OC21	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC21
SUBJECT_SAMPLE_FACTORS           	-	OC22	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC22
SUBJECT_SAMPLE_FACTORS           	-	OC23	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC23
SUBJECT_SAMPLE_FACTORS           	-	OC24	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC24
SUBJECT_SAMPLE_FACTORS           	-	OC25	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC25
SUBJECT_SAMPLE_FACTORS           	-	OC26	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC26
SUBJECT_SAMPLE_FACTORS           	-	OC27	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC27
SUBJECT_SAMPLE_FACTORS           	-	OC28	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC28
SUBJECT_SAMPLE_FACTORS           	-	OC29	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC29
SUBJECT_SAMPLE_FACTORS           	-	OC30	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC30
SUBJECT_SAMPLE_FACTORS           	-	OC31	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC31
SUBJECT_SAMPLE_FACTORS           	-	OC32	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC32
SUBJECT_SAMPLE_FACTORS           	-	OC33	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC33
SUBJECT_SAMPLE_FACTORS           	-	OC34	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC34
SUBJECT_SAMPLE_FACTORS           	-	OC35	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC35
SUBJECT_SAMPLE_FACTORS           	-	OC36	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC36
SUBJECT_SAMPLE_FACTORS           	-	OC37	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC37
SUBJECT_SAMPLE_FACTORS           	-	OC38	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC38
SUBJECT_SAMPLE_FACTORS           	-	OC39	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC39
SUBJECT_SAMPLE_FACTORS           	-	OC40	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC40
SUBJECT_SAMPLE_FACTORS           	-	OC41	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC41
SUBJECT_SAMPLE_FACTORS           	-	OC42	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC42
SUBJECT_SAMPLE_FACTORS           	-	OC43	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC43
SUBJECT_SAMPLE_FACTORS           	-	OC44	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC44
SUBJECT_SAMPLE_FACTORS           	-	OC45	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC45
SUBJECT_SAMPLE_FACTORS           	-	OC46	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC46
SUBJECT_SAMPLE_FACTORS           	-	OC47	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC47
SUBJECT_SAMPLE_FACTORS           	-	OC48	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC48
SUBJECT_SAMPLE_FACTORS           	-	OC49	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC49
SUBJECT_SAMPLE_FACTORS           	-	OC50	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC50
SUBJECT_SAMPLE_FACTORS           	-	OC51	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC51
SUBJECT_SAMPLE_FACTORS           	-	OC52	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC52
SUBJECT_SAMPLE_FACTORS           	-	OC53	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC53
SUBJECT_SAMPLE_FACTORS           	-	OC54	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC54
SUBJECT_SAMPLE_FACTORS           	-	OC55	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC55
SUBJECT_SAMPLE_FACTORS           	-	OC56	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC56
SUBJECT_SAMPLE_FACTORS           	-	OC57	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC57
SUBJECT_SAMPLE_FACTORS           	-	OC58	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC58
SUBJECT_SAMPLE_FACTORS           	-	OC59	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC59
SUBJECT_SAMPLE_FACTORS           	-	OC60	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC60
SUBJECT_SAMPLE_FACTORS           	-	OC61	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC61
SUBJECT_SAMPLE_FACTORS           	-	OC62	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC62
SUBJECT_SAMPLE_FACTORS           	-	OC63	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC63
SUBJECT_SAMPLE_FACTORS           	-	OC64	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC64
SUBJECT_SAMPLE_FACTORS           	-	OC65	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC65
SUBJECT_SAMPLE_FACTORS           	-	OC66	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC66
SUBJECT_SAMPLE_FACTORS           	-	OC67	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC67
SUBJECT_SAMPLE_FACTORS           	-	OC68	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC68
SUBJECT_SAMPLE_FACTORS           	-	OC69	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC69
SUBJECT_SAMPLE_FACTORS           	-	OC70	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC70
SUBJECT_SAMPLE_FACTORS           	-	OC71	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC71
SUBJECT_SAMPLE_FACTORS           	-	OC72	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC72
SUBJECT_SAMPLE_FACTORS           	-	OC73	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC73
SUBJECT_SAMPLE_FACTORS           	-	OC74	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC74
SUBJECT_SAMPLE_FACTORS           	-	OC75	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC75
SUBJECT_SAMPLE_FACTORS           	-	OC76	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC76
SUBJECT_SAMPLE_FACTORS           	-	OC77	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC77
SUBJECT_SAMPLE_FACTORS           	-	OC78	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC78
SUBJECT_SAMPLE_FACTORS           	-	OC79	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC79
SUBJECT_SAMPLE_FACTORS           	-	OC80	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC80
SUBJECT_SAMPLE_FACTORS           	-	OC81	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC81
SUBJECT_SAMPLE_FACTORS           	-	OC82	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC82
SUBJECT_SAMPLE_FACTORS           	-	OC83	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC83
SUBJECT_SAMPLE_FACTORS           	-	OC84	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC84
SUBJECT_SAMPLE_FACTORS           	-	OC85	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC85
SUBJECT_SAMPLE_FACTORS           	-	OC86	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC86
SUBJECT_SAMPLE_FACTORS           	-	OC87	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC87
SUBJECT_SAMPLE_FACTORS           	-	OC88	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC88
SUBJECT_SAMPLE_FACTORS           	-	OC89	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC89
SUBJECT_SAMPLE_FACTORS           	-	OC90	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC90
SUBJECT_SAMPLE_FACTORS           	-	OC91	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC91
SUBJECT_SAMPLE_FACTORS           	-	OC92	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC92
SUBJECT_SAMPLE_FACTORS           	-	OC93	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC93
SUBJECT_SAMPLE_FACTORS           	-	OC94	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC94
SUBJECT_SAMPLE_FACTORS           	-	OC95	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC95
SUBJECT_SAMPLE_FACTORS           	-	OC96	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC96
SUBJECT_SAMPLE_FACTORS           	-	OC97	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC97
SUBJECT_SAMPLE_FACTORS           	-	OC98	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC98
SUBJECT_SAMPLE_FACTORS           	-	OC99	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC99
SUBJECT_SAMPLE_FACTORS           	-	OC100	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC100
SUBJECT_SAMPLE_FACTORS           	-	OC101	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC101
SUBJECT_SAMPLE_FACTORS           	-	OC102	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC102
SUBJECT_SAMPLE_FACTORS           	-	OC103	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC103
SUBJECT_SAMPLE_FACTORS           	-	OC104	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC104
SUBJECT_SAMPLE_FACTORS           	-	OC105	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC105
SUBJECT_SAMPLE_FACTORS           	-	OC106	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC106
SUBJECT_SAMPLE_FACTORS           	-	OC107	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC107
SUBJECT_SAMPLE_FACTORS           	-	OC108	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC108
SUBJECT_SAMPLE_FACTORS           	-	OC109	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC109
SUBJECT_SAMPLE_FACTORS           	-	OC110	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC110
SUBJECT_SAMPLE_FACTORS           	-	OC111	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC111
SUBJECT_SAMPLE_FACTORS           	-	OC112	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC112
SUBJECT_SAMPLE_FACTORS           	-	OC113	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC113
SUBJECT_SAMPLE_FACTORS           	-	OC114	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC114
SUBJECT_SAMPLE_FACTORS           	-	OC115	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC115
SUBJECT_SAMPLE_FACTORS           	-	OC116	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC116
SUBJECT_SAMPLE_FACTORS           	-	OC117	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC117
SUBJECT_SAMPLE_FACTORS           	-	OC118	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC118
SUBJECT_SAMPLE_FACTORS           	-	OC119	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC119
SUBJECT_SAMPLE_FACTORS           	-	OC120	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC120
SUBJECT_SAMPLE_FACTORS           	-	OC121	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC121
SUBJECT_SAMPLE_FACTORS           	-	OC122	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC122
SUBJECT_SAMPLE_FACTORS           	-	OC123	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC123
SUBJECT_SAMPLE_FACTORS           	-	OC124	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC124
SUBJECT_SAMPLE_FACTORS           	-	OC125	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC125
SUBJECT_SAMPLE_FACTORS           	-	OC126	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC126
SUBJECT_SAMPLE_FACTORS           	-	OC127	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC127
SUBJECT_SAMPLE_FACTORS           	-	OC128	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC128
SUBJECT_SAMPLE_FACTORS           	-	OC129	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC129
SUBJECT_SAMPLE_FACTORS           	-	OC130	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC130
SUBJECT_SAMPLE_FACTORS           	-	OC131	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC131
SUBJECT_SAMPLE_FACTORS           	-	OC132	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC132
SUBJECT_SAMPLE_FACTORS           	-	OC133	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC133
SUBJECT_SAMPLE_FACTORS           	-	OC134	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC134
SUBJECT_SAMPLE_FACTORS           	-	OC135	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC135
SUBJECT_SAMPLE_FACTORS           	-	OC136	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC136
SUBJECT_SAMPLE_FACTORS           	-	OC137	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC137
SUBJECT_SAMPLE_FACTORS           	-	OC138	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC138
SUBJECT_SAMPLE_FACTORS           	-	OC139	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC139
SUBJECT_SAMPLE_FACTORS           	-	OC140	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC140
SUBJECT_SAMPLE_FACTORS           	-	OC141	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC141
SUBJECT_SAMPLE_FACTORS           	-	OC142	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC142
SUBJECT_SAMPLE_FACTORS           	-	OC143	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC143
SUBJECT_SAMPLE_FACTORS           	-	OC144	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC144
SUBJECT_SAMPLE_FACTORS           	-	OC145	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC145
SUBJECT_SAMPLE_FACTORS           	-	OC146	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC146
SUBJECT_SAMPLE_FACTORS           	-	OC147	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC147
SUBJECT_SAMPLE_FACTORS           	-	OC148	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC148
SUBJECT_SAMPLE_FACTORS           	-	OC149	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC149
SUBJECT_SAMPLE_FACTORS           	-	OC150	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC150
SUBJECT_SAMPLE_FACTORS           	-	OC151	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC151
SUBJECT_SAMPLE_FACTORS           	-	OC152	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC152
SUBJECT_SAMPLE_FACTORS           	-	OC153	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC153
SUBJECT_SAMPLE_FACTORS           	-	OC154	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC154
SUBJECT_SAMPLE_FACTORS           	-	OC155	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC155
SUBJECT_SAMPLE_FACTORS           	-	OC156	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC156
SUBJECT_SAMPLE_FACTORS           	-	OC157	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC157
SUBJECT_SAMPLE_FACTORS           	-	OC158	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC158
SUBJECT_SAMPLE_FACTORS           	-	OC159	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC159
SUBJECT_SAMPLE_FACTORS           	-	OC160	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC160
SUBJECT_SAMPLE_FACTORS           	-	OC161	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC161
SUBJECT_SAMPLE_FACTORS           	-	OC162	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC162
SUBJECT_SAMPLE_FACTORS           	-	OC163	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC163
SUBJECT_SAMPLE_FACTORS           	-	OC164	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC164
SUBJECT_SAMPLE_FACTORS           	-	OC165	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC165
SUBJECT_SAMPLE_FACTORS           	-	OC166	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC166
SUBJECT_SAMPLE_FACTORS           	-	OC167	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC167
SUBJECT_SAMPLE_FACTORS           	-	OC168	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC168
SUBJECT_SAMPLE_FACTORS           	-	OC169	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC169
SUBJECT_SAMPLE_FACTORS           	-	OC170	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC170
SUBJECT_SAMPLE_FACTORS           	-	OC171	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC171
SUBJECT_SAMPLE_FACTORS           	-	OC172	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC172
SUBJECT_SAMPLE_FACTORS           	-	OC173	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC173
SUBJECT_SAMPLE_FACTORS           	-	OC174	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC174
SUBJECT_SAMPLE_FACTORS           	-	OC175	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC175
SUBJECT_SAMPLE_FACTORS           	-	OC176	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC176
SUBJECT_SAMPLE_FACTORS           	-	OC177	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC177
SUBJECT_SAMPLE_FACTORS           	-	OC178	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC178
SUBJECT_SAMPLE_FACTORS           	-	OC179	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC179
SUBJECT_SAMPLE_FACTORS           	-	OC180	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC180
SUBJECT_SAMPLE_FACTORS           	-	OC181	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC181
SUBJECT_SAMPLE_FACTORS           	-	OC182	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC182
SUBJECT_SAMPLE_FACTORS           	-	OC183	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC183
SUBJECT_SAMPLE_FACTORS           	-	OC184	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC184
SUBJECT_SAMPLE_FACTORS           	-	OC185	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC185
SUBJECT_SAMPLE_FACTORS           	-	OC186	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC186
SUBJECT_SAMPLE_FACTORS           	-	OC187	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC187
SUBJECT_SAMPLE_FACTORS           	-	OC188	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC188
SUBJECT_SAMPLE_FACTORS           	-	OC189	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC189
SUBJECT_SAMPLE_FACTORS           	-	OC190	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC190
SUBJECT_SAMPLE_FACTORS           	-	OC191	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC191
SUBJECT_SAMPLE_FACTORS           	-	OC192	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC192
SUBJECT_SAMPLE_FACTORS           	-	OC193	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC193
SUBJECT_SAMPLE_FACTORS           	-	OC194	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC194
SUBJECT_SAMPLE_FACTORS           	-	OC195	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC195
SUBJECT_SAMPLE_FACTORS           	-	OC196	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC196
SUBJECT_SAMPLE_FACTORS           	-	OC197	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC197
SUBJECT_SAMPLE_FACTORS           	-	OC198	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC198
SUBJECT_SAMPLE_FACTORS           	-	OC199	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC199
SUBJECT_SAMPLE_FACTORS           	-	OC200	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC200
SUBJECT_SAMPLE_FACTORS           	-	OC201	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC201
SUBJECT_SAMPLE_FACTORS           	-	OC202	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC202
SUBJECT_SAMPLE_FACTORS           	-	OC203	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC203
SUBJECT_SAMPLE_FACTORS           	-	OC204	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC204
SUBJECT_SAMPLE_FACTORS           	-	OC205	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC205
SUBJECT_SAMPLE_FACTORS           	-	OC206	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC206
SUBJECT_SAMPLE_FACTORS           	-	OC207	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC207
SUBJECT_SAMPLE_FACTORS           	-	OC208	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC208
SUBJECT_SAMPLE_FACTORS           	-	OC209	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC209
SUBJECT_SAMPLE_FACTORS           	-	OC210	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC210
SUBJECT_SAMPLE_FACTORS           	-	OC211	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC211
SUBJECT_SAMPLE_FACTORS           	-	OC212	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC212
SUBJECT_SAMPLE_FACTORS           	-	OC213	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC213
SUBJECT_SAMPLE_FACTORS           	-	OC214	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC214
SUBJECT_SAMPLE_FACTORS           	-	OC215	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC215
SUBJECT_SAMPLE_FACTORS           	-	OC216	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC216
SUBJECT_SAMPLE_FACTORS           	-	OC217	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC217
SUBJECT_SAMPLE_FACTORS           	-	OC218	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC218
SUBJECT_SAMPLE_FACTORS           	-	OC219	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC219
SUBJECT_SAMPLE_FACTORS           	-	OC220	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC220
SUBJECT_SAMPLE_FACTORS           	-	OC221	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC221
SUBJECT_SAMPLE_FACTORS           	-	OC222	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC222
SUBJECT_SAMPLE_FACTORS           	-	OC223	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC223
SUBJECT_SAMPLE_FACTORS           	-	OC224	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC224
SUBJECT_SAMPLE_FACTORS           	-	OC225	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC225
SUBJECT_SAMPLE_FACTORS           	-	OC226	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC226
SUBJECT_SAMPLE_FACTORS           	-	OC227	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC227
SUBJECT_SAMPLE_FACTORS           	-	OC228	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC228
SUBJECT_SAMPLE_FACTORS           	-	OC229	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC229
SUBJECT_SAMPLE_FACTORS           	-	OC230	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC230
SUBJECT_SAMPLE_FACTORS           	-	OC231	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC231
SUBJECT_SAMPLE_FACTORS           	-	OC232	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC232
SUBJECT_SAMPLE_FACTORS           	-	OC233	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC233
SUBJECT_SAMPLE_FACTORS           	-	OC234	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC234
SUBJECT_SAMPLE_FACTORS           	-	OC235	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC235
SUBJECT_SAMPLE_FACTORS           	-	OC236	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC236
SUBJECT_SAMPLE_FACTORS           	-	OC237	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC237
SUBJECT_SAMPLE_FACTORS           	-	OC238	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC238
SUBJECT_SAMPLE_FACTORS           	-	OC239	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC239
SUBJECT_SAMPLE_FACTORS           	-	OC240	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC240
SUBJECT_SAMPLE_FACTORS           	-	OC241	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC241
SUBJECT_SAMPLE_FACTORS           	-	OC242	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC242
SUBJECT_SAMPLE_FACTORS           	-	OC243	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC243
SUBJECT_SAMPLE_FACTORS           	-	OC244	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC244
SUBJECT_SAMPLE_FACTORS           	-	OC245	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC245
SUBJECT_SAMPLE_FACTORS           	-	OC246	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC246
SUBJECT_SAMPLE_FACTORS           	-	OC247	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC247
SUBJECT_SAMPLE_FACTORS           	-	OC248	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC248
SUBJECT_SAMPLE_FACTORS           	-	OC249	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC249
SUBJECT_SAMPLE_FACTORS           	-	OC250	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC250
SUBJECT_SAMPLE_FACTORS           	-	OC251	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC251
SUBJECT_SAMPLE_FACTORS           	-	OC252	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC252
SUBJECT_SAMPLE_FACTORS           	-	OC253	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC253
SUBJECT_SAMPLE_FACTORS           	-	OC254	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC254
SUBJECT_SAMPLE_FACTORS           	-	OC255	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC255
SUBJECT_SAMPLE_FACTORS           	-	OC256	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC256
SUBJECT_SAMPLE_FACTORS           	-	OC257	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC257
SUBJECT_SAMPLE_FACTORS           	-	OC258	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC258
SUBJECT_SAMPLE_FACTORS           	-	OC259	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC259
SUBJECT_SAMPLE_FACTORS           	-	OC260	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC260
SUBJECT_SAMPLE_FACTORS           	-	OC261	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC261
SUBJECT_SAMPLE_FACTORS           	-	OC262	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC262
SUBJECT_SAMPLE_FACTORS           	-	OC263	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC263
SUBJECT_SAMPLE_FACTORS           	-	OC264	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC264
SUBJECT_SAMPLE_FACTORS           	-	OC265	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC265
SUBJECT_SAMPLE_FACTORS           	-	OC266	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC266
SUBJECT_SAMPLE_FACTORS           	-	OC267	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC267
SUBJECT_SAMPLE_FACTORS           	-	OC268	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC268
SUBJECT_SAMPLE_FACTORS           	-	OC269	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC269
SUBJECT_SAMPLE_FACTORS           	-	OC270	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC270
SUBJECT_SAMPLE_FACTORS           	-	OC271	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC271
SUBJECT_SAMPLE_FACTORS           	-	OC272	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC272
SUBJECT_SAMPLE_FACTORS           	-	OC273	Treatment:Ovarian Cancer	RAW_FILE_NAME=OC273
SUBJECT_SAMPLE_FACTORS           	-	B1	Treatment:BRACA positive	RAW_FILE_NAME=B1
SUBJECT_SAMPLE_FACTORS           	-	B2	Treatment:BRACA positive	RAW_FILE_NAME=B2
SUBJECT_SAMPLE_FACTORS           	-	B3	Treatment:BRACA positive	RAW_FILE_NAME=B3
#COLLECTION
CO:COLLECTION_SUMMARY            	Serum samples were collected from two independent tissue banks in South Korea,
CO:COLLECTION_SUMMARY            	Dongsan Hospital Human Tissue Bank and Gangnam Severance Hospital Gene Bank. The
CO:COLLECTION_SUMMARY            	Severance cohort included 185 samples from ovarian cancer patients, 47 from
CO:COLLECTION_SUMMARY            	women with benign ovarian tumor, 50 from invasive cervical cancer and 21 samples
CO:COLLECTION_SUMMARY            	from patients with benign uterine tumor. Blood was collected from all patients
CO:COLLECTION_SUMMARY            	during surgery after anesthesia and at least 8 hours of fasting. In the Dongsan
CO:COLLECTION_SUMMARY            	cohort 88 women had ovarian cancer, 12 had benign ovarian tumor, 10 had benign
CO:COLLECTION_SUMMARY            	uterine tumor, and 9 women had cervical cancer. As with the Severance cohort,
CO:COLLECTION_SUMMARY            	samples from these patients were collected during surgery after anesthesia and
CO:COLLECTION_SUMMARY            	at least 6 hours of fasting. All recruited participants were of Korean descent.
CO:COLLECTION_SUMMARY            	Samples from both cohorts were grouped together and patients with ovarian cancer
CO:COLLECTION_SUMMARY            	(OC) and all other gynecological malignancies (non-OC) were age matched. The
CO:COLLECTION_SUMMARY            	matched cohort included 208 patients with ovarian cancer (mean age 51.9 years)
CO:COLLECTION_SUMMARY            	and 137 non-OC patients (mean age 49.9 years). Disease stages and histological
CO:COLLECTION_SUMMARY            	characteristics are given in Table 1. Among the OC patients, 93 patients had
CO:COLLECTION_SUMMARY            	early stage (I and II) cancer. Ten of the OC patients had recurrent cancer and 9
CO:COLLECTION_SUMMARY            	patients have had their samples collected more than once. In addition to
CO:COLLECTION_SUMMARY            	diseased patients, samples from normal controls (women with no known
CO:COLLECTION_SUMMARY            	gynecological malignancies) were collected during regular health exams at
CO:COLLECTION_SUMMARY            	Severance hospital. In this case, blood was collected at least 8 hours after
CO:COLLECTION_SUMMARY            	fasting.
CO:SAMPLE_TYPE                   	Blood (serum)
#TREATMENT
TR:TREATMENT_SUMMARY             	Serum samples were collected from two independent tissue banks in South Korea,
TR:TREATMENT_SUMMARY             	Dongsan Hospital Human Tissue Bank and Gangnam Severance Hospital Gene Bank. The
TR:TREATMENT_SUMMARY             	Severance cohort included 185 samples from ovarian cancer patients, 47 from
TR:TREATMENT_SUMMARY             	women with benign ovarian tumor, 50 from invasive cervical cancer and 21 samples
TR:TREATMENT_SUMMARY             	from patients with benign uterine tumor. Blood was collected from all patients
TR:TREATMENT_SUMMARY             	during surgery after anesthesia and at least 8 hours of fasting. In the Dongsan
TR:TREATMENT_SUMMARY             	cohort 88 women had ovarian cancer, 12 had benign ovarian tumor, 10 had benign
TR:TREATMENT_SUMMARY             	uterine tumor, and 9 women had cervical cancer. As with the Severance cohort,
TR:TREATMENT_SUMMARY             	samples from these patients were collected during surgery after anesthesia and
TR:TREATMENT_SUMMARY             	at least 6 hours of fasting. All recruited participants were of Korean descent.
TR:TREATMENT_SUMMARY             	Samples from both cohorts were grouped together and patients with ovarian cancer
TR:TREATMENT_SUMMARY             	(OC) and all other gynecological malignancies (non-OC) were age matched. The
TR:TREATMENT_SUMMARY             	matched cohort included 208 patients with ovarian cancer (mean age 51.9 years)
TR:TREATMENT_SUMMARY             	and 137 non-OC patients (mean age 49.9 years). Among the OC patients, 93
TR:TREATMENT_SUMMARY             	patients had early stage (I and II) cancer. Ten of the OC patients had recurrent
TR:TREATMENT_SUMMARY             	cancer and 9 patients have had their samples collected more than once. In
TR:TREATMENT_SUMMARY             	addition to diseased patients, samples from normal controls (women with no known
TR:TREATMENT_SUMMARY             	gynecological malignancies) were collected during regular health exams at
TR:TREATMENT_SUMMARY             	Severance hospital. In this case, blood was collected at least 8 hours after
TR:TREATMENT_SUMMARY             	fasting. As noted earlier, blood from diseased patients was collected after the
TR:TREATMENT_SUMMARY             	initiation of anesthesia, and thus could not be directly compared with normal
TR:TREATMENT_SUMMARY             	controls without introducing major confounding effects. Therefore, control
TR:TREATMENT_SUMMARY             	samples were excluded from our main analysis. However, for information purposes
TR:TREATMENT_SUMMARY             	only, we also provide a lipidome comparison between healthy controls and OC
TR:TREATMENT_SUMMARY             	patients in supplemental information.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Serum samples were thawed on ice, followed by metabolite extraction of the
SP:SAMPLEPREP_SUMMARY            	non-polar (lipids) metabolome. Extraction solvent was prepared by adding 725 μL
SP:SAMPLEPREP_SUMMARY            	of the isotopically labeled lipid standard mixture to 43.5 mL 2-propanol (1:60
SP:SAMPLEPREP_SUMMARY            	ratio) and was kept on ice. This cold extraction solvent was added to serum
SP:SAMPLEPREP_SUMMARY            	samples in a 3:1 ratio (solvent: serum) for protein precipitation followed by
SP:SAMPLEPREP_SUMMARY            	vortex mixing for 15 seconds. Samples were centrifuged at 13,000 rpm for 7
SP:SAMPLEPREP_SUMMARY            	minutes and the resulting supernatant was transferred to LC vials. The
SP:SAMPLEPREP_SUMMARY            	supernatant was stored at -80 °C until UHPLC-MS analysis, which was performed
SP:SAMPLEPREP_SUMMARY            	within one week of preparation. A blank sample, prepared with LC-MS grade water,
SP:SAMPLEPREP_SUMMARY            	underwent the same sample preparation process as the serum samples and was
SP:SAMPLEPREP_SUMMARY            	analyzed with the rest of the samples. Pooled quality control (QC) samples were
SP:SAMPLEPREP_SUMMARY            	prepared by combining 5-10 μL aliquots of the supernatant of each serum sample.
SP:SAMPLEPREP_SUMMARY            	This pooled QC sample was analyzed after every 10 LC-MS runs to monitor
SP:SAMPLEPREP_SUMMARY            	instrument stability through the course of the experiment. Samples were
SP:SAMPLEPREP_SUMMARY            	randomized for sample preparation and were run in a randomized order on
SP:SAMPLEPREP_SUMMARY            	consecutive days.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	Thermo Accucore C30 (150 x 2.1mm,2.6um)
CH:SOLVENT_A                     	10 mM ammonium formate with water/acetonitrile (40:60 v/v) and 0.1% formic acid
CH:SOLVENT_B                     	10 mM ammonium formate with 2-isopropanol/acetonitrile (90:10 v/v) and 0.1%
CH:SOLVENT_B                     	formic acid
CH:FLOW_GRADIENT                 	0-1 min 80-40% A; 1-5 min 40-30% A; 5-5.5 min 30-15% A; 5.5-8 min 15-10% A;
CH:FLOW_GRADIENT                 	8-8.2 min 10-0% A; 8.2-10.5 0% A; 10.5-10.7 min 0-80% A; 10.7-12.0 min 80% A.
CH:FLOW_RATE                     	0.4 ml min-1
CH:COLUMN_TEMPERATURE            	50
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Orbitrap ID-X tribrid
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	MS data were acquired in the 150-2000 m/z range with a 120,000 mass resolution
MS:MS_COMMENTS                   	setting. The most relevant MS parameters and the chromatographic gradient used
MS:MS_COMMENTS                   	are given in supplementary section Table S2 and S3, respectively. For MS/MS
MS:MS_COMMENTS                   	experiments, the Thermo Scientific Deep AcquireX data acquisition workflow was
MS:MS_COMMENTS                   	performed. Stepped normalized collision energy (NCE) of 15,30,45 was used for
MS:MS_COMMENTS                   	fragmenting precursor ions in the HCD cell followed by Orbitrap analysis at
MS:MS_COMMENTS                   	30,000 mass resolving power. Precursor ions were also fragmented with CID energy
MS:MS_COMMENTS                   	of 40 and were analyzed in the ion trap.
MS:MS_RESULTS_FILE               	ST002521_AN004154_Results.txt	UNITS:chromatographic peak area 	Has m/z:Neutral masses	Has RT:Yes	RT units:Minutes
#END