#METABOLOMICS WORKBENCH silviaradenkovic_20230418_143045 DATATRACK_ID:3870 STUDY_ID:ST002566 ANALYSIS_ID:AN004227 PROJECT_ID:PR001653
VERSION             	1
CREATED_ON             	April 18, 2023, 5:28 pm
#PROJECT
PR:PROJECT_TITLE                 	Metabolomic profiling of PMM2-CDG patient fibroblasts
PR:PROJECT_SUMMARY               	Abnormal polyol metabolism has been predominantly associated with diabetes,
PR:PROJECT_SUMMARY               	where excess glucose is converted to sorbitol by aldose reductase (AR).
PR:PROJECT_SUMMARY               	Recently, abnormal polyol metabolism has also been implicated in
PR:PROJECT_SUMMARY               	phosphomannomutase 2-congenital disorder of glycosylation (PMM2-CDG), and
PR:PROJECT_SUMMARY               	epalrestat, an AR inhibitor, proposed as a potential therapy for this disorder.
PR:PROJECT_SUMMARY               	Given that the PMM enzyme is not closely connected to polyol metabolism, and,
PR:PROJECT_SUMMARY               	unlike in diabetes, PMM2-CDG does not present with hyperglycemia in blood, the
PR:PROJECT_SUMMARY               	increased polyol production, and the therapeutic mechanism of epalrestat in
PR:PROJECT_SUMMARY               	PMM2-CDG remained largely elusive. PMM2-CDG is caused by deficiency of the PMM
PR:PROJECT_SUMMARY               	enzyme and results in a depletion of mannose-1-P and guanosine diphosphate
PR:PROJECT_SUMMARY               	mannose (GDP-mannose), which is essential for glycosylation. Here, we show that
PR:PROJECT_SUMMARY               	apart from glycosylation abnormalities, PMM2 deficiency also leads to changes in
PR:PROJECT_SUMMARY               	intracellular glucose flux, which results in an increase in intracellular
PR:PROJECT_SUMMARY               	polyols.
PR:INSTITUTE                     	Mayo Clinic
PR:LAST_NAME                     	Radenkovic
PR:FIRST_NAME                    	Silvia
PR:ADDRESS                       	200 2nd Ave SW Rochester MN
PR:EMAIL                         	radenkovic.silvia@mayo.edu
PR:PHONE                         	507(77) 6-6107
PR:FUNDING_SOURCE                	NIH, KU Leuven
#STUDY
ST:STUDY_TITLE                   	Metabolomic profiling of PMM2-CDG patient fibroblasts
ST:STUDY_SUMMARY                 	Abnormal polyol metabolism has been predominantly associated with diabetes,
ST:STUDY_SUMMARY                 	where excess glucose is converted to sorbitol by aldose reductase (AR).
ST:STUDY_SUMMARY                 	Recently, abnormal polyol metabolism has also been implicated in
ST:STUDY_SUMMARY                 	phosphomannomutase 2-congenital disorder of glycosylation (PMM2-CDG), and
ST:STUDY_SUMMARY                 	epalrestat, an AR inhibitor, proposed as a potential therapy for this disorder.
ST:STUDY_SUMMARY                 	Given that the PMM enzyme is not closely connected to polyol metabolism, and,
ST:STUDY_SUMMARY                 	unlike in diabetes, PMM2-CDG does not present with hyperglycemia in blood, the
ST:STUDY_SUMMARY                 	increased polyol production, and the therapeutic mechanism of epalrestat in
ST:STUDY_SUMMARY                 	PMM2-CDG remained largely elusive. PMM2-CDG is caused by deficiency of the PMM
ST:STUDY_SUMMARY                 	enzyme and results in a depletion of mannose-1-P and guanosine diphosphate
ST:STUDY_SUMMARY                 	mannose (GDP-mannose), which is essential for glycosylation. Here, we show that
ST:STUDY_SUMMARY                 	apart from glycosylation abnormalities, PMM2 deficiency also leads to changes in
ST:STUDY_SUMMARY                 	intracellular glucose flux, which results in an increase in intracellular
ST:STUDY_SUMMARY                 	polyols.
ST:INSTITUTE                     	Mayo Clinic
ST:LAST_NAME                     	Radenkovic
ST:FIRST_NAME                    	Silvia
ST:ADDRESS                       	200 2nd Ave SW Rochester MN, USA
ST:EMAIL                         	radenkovic.silvia@mayo.edu
ST:PHONE                         	507(77) 6-6107
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
SU:GENOTYPE_STRAIN               	WT/PMM2-CDG
SU:AGE_OR_AGE_RANGE              	5-45
SU:GENDER                        	Male and female
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	P1	SR01 (1)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (1); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	P1	SR01 (2)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (2); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	P1	SR01 (3)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (3); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	P1	SR01 (4)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (4); Medium=5.5mM GLU +
SUBJECT_SAMPLE_FACTORS           	P2	SR01 (5)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (5); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	P2	SR01 (6)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (6); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	P2	SR01 (7)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (7); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	P2	SR01 (8)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (8); Medium=5.5mM GLU +
SUBJECT_SAMPLE_FACTORS           	P4	SR01 (9)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (9); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	P4	SR01 (10)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (10); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	P4	SR01 (11)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (11); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	P4	SR01 (12)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (12); Medium=5.5mM GLU +
SUBJECT_SAMPLE_FACTORS           	P5	SR01 (13)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (13); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	P5	SR01 (14)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (14); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	P5	SR01 (15)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (15); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	P5	SR01 (16)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (16); Medium=5.5mM GLU +
SUBJECT_SAMPLE_FACTORS           	P6	SR01 (17)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (17); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	P6	SR01 (18)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (18); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	P6	SR01 (19)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (19); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	P6	SR01 (20)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (20); Medium=5.5mM GLU +
SUBJECT_SAMPLE_FACTORS           	C1	SR01 (21)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (21); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	C1	SR01 (22)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (22); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	C1	SR01 (23)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (23); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	C1	SR01 (24)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (24); Medium=5.5mM GLU +
SUBJECT_SAMPLE_FACTORS           	C3	SR01 (25)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (25); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	C3	SR01 (26)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (26); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	C3	SR01 (27)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (27); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	C3	SR01 (28)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (28); Medium=5.5mM GLU +
SUBJECT_SAMPLE_FACTORS           	C4	SR01 (29)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (29); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	C4	SR01 (30)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (30); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	C4	SR01 (31)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (31); Medium=5.5mM 13C GLU
SUBJECT_SAMPLE_FACTORS           	C4	SR01 (32)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR01 (32); Medium=5.5mM GLU +
SUBJECT_SAMPLE_FACTORS           	P1	SR02 (1)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (1); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P1	SR02 (2)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (2); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P1	SR02 (3)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (3); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P1	SR02 (4)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (4); Medium=5.5 mM 12 GLU
SUBJECT_SAMPLE_FACTORS           	P2	SR02 (5)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (5); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P2	SR02 (6)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (6); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P2	SR02 (7)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (7); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P2	SR02 (8)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (8); Medium=5.5 mM 12 GLU
SUBJECT_SAMPLE_FACTORS           	P3	SR02 (9)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (9); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P3	SR02 (10)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (10); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P3	SR02 (11)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (11); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P3	SR02 (12)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (12); Medium=5.5 mM 12 GLU
SUBJECT_SAMPLE_FACTORS           	C2	SR02 (13)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (13); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	C2	SR02 (14)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (14); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	C2	SR02 (15)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (15); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	C2	SR02 (16)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (16); Medium=5.5 mM 12 GLU
SUBJECT_SAMPLE_FACTORS           	C3	SR02 (17)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (17); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	C3	SR02 (18)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (18); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	C3	SR02 (19)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (19); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	C3	SR02 (20)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR02 (20); Medium=5.5 mM 12 GLU
SUBJECT_SAMPLE_FACTORS           	P1	SR03 (1)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (1); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P1	SR03 (2)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (2); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P1	SR03 (3)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (3); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P1	SR03 (4)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (4); Medium=5.5 mM 12 GLU
SUBJECT_SAMPLE_FACTORS           	P2	SR03 (5)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (5); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P2	SR03 (6)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (6); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P2	SR03 (7)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (7); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P2	SR03 (8)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (8); Medium=5.5 mM 12 GLU
SUBJECT_SAMPLE_FACTORS           	P3	SR03 (9)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (9); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P3	SR03 (10)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (10); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P3	SR03 (11)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (11); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	P3	SR03 (12)	Genotype:PMM2-CDG	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (12); Medium=5.5 mM 12 GLU
SUBJECT_SAMPLE_FACTORS           	C5	SR03 (13)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (13); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	C5	SR03 (14)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (14); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	C5	SR03 (15)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (15); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	C5	SR03 (16)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (16); Medium=5.5 mM 12 GLU
SUBJECT_SAMPLE_FACTORS           	C3	SR03 (17)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (17); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	C3	SR03 (18)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (18); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	C3	SR03 (19)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (19); Medium=5.5 mM 13 GLU
SUBJECT_SAMPLE_FACTORS           	C3	SR03 (20)	Genotype:WT	Cell type=Skin fibroblasts; RAW_FILE_NAME=SR03 (20); Medium=5.5 mM 12 GLU
#COLLECTION
CO:COLLECTION_SUMMARY            	Cells were washed with PBS, cells were incubated with extraction buffer for 2min
CO:COLLECTION_SUMMARY            	before scraping and transferring to a fresh eppendorf. Samples were precipitated
CO:COLLECTION_SUMMARY            	overnight at -80, then they were centrifuged at max rpm, 20min, 4 degrees C and
CO:COLLECTION_SUMMARY            	supernatant transferred to an M/S vial.
CO:SAMPLE_TYPE                   	Fibroblasts
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	Cells were grown in medium with 13c glucose or 12c glucose
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Metabolites were extracted with 80% Methanol and IS (d27 myristic acid)
SP:PROCESSING_STORAGE_CONDITIONS 	-80℃
SP:EXTRACT_STORAGE               	-80℃
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	C18 iP REVERSE PHASE
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Waters Acquity
CH:COLUMN_NAME                   	Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)
CH:SOLVENT_A                     	100% water; 10mM tributylamine; 15mM acetic acid
CH:SOLVENT_B                     	100% methanol
CH:FLOW_GRADIENT                 	The gradient started with 5% of solvent B and 95% solvent A and remained at 5% B
CH:FLOW_GRADIENT                 	until 2 min post injection. A linear gradient to 37% B was carried out until 7
CH:FLOW_GRADIENT                 	min and increased to 41% until 14 min. Between 14 and 26 minutes the gradient
CH:FLOW_GRADIENT                 	increased to 95% of B and remained at 95% B for 4 minutes. At 30 min the
CH:FLOW_GRADIENT                 	gradient returned to 5% B. The chromatography was stopped at 40 min
CH:FLOW_RATE                     	0.25 ml/min
CH:COLUMN_TEMPERATURE            	40
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	El-Maven polly, ThermoFisher Xcalibur, Metabolites were annotated based on the
MS:MS_COMMENTS                   	in-house metabolite library- elution time and m/z values
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	AUC
MS_METABOLITE_DATA_START
Samples	SR01 (1)	SR01 (2)	SR01 (3)	SR01 (4)	SR01 (5)	SR01 (6)	SR01 (7)	SR01 (8)	SR01 (9)	SR01 (10)	SR01 (11)	SR01 (12)	SR01 (13)	SR01 (14)	SR01 (15)	SR01 (16)	SR01 (17)	SR01 (18)	SR01 (19)	SR01 (20)	SR01 (21)	SR01 (22)	SR01 (23)	SR01 (24)	SR01 (25)	SR01 (26)	SR01 (27)	SR01 (28)	SR01 (29)	SR01 (30)	SR01 (31)	SR01 (32)	SR02 (1)	SR02 (2)	SR02 (3)	SR02 (4)	SR02 (5)	SR02 (6)	SR02 (7)	SR02 (8)	SR02 (9)	SR02 (10)	SR02 (11)	SR02 (12)	SR02 (13)	SR02 (14)	SR02 (15)	SR02 (16)	SR02 (17)	SR02 (18)	SR02 (19)	SR02 (20)	SR03 (1)	SR03 (2)	SR03 (3)	SR03 (4)	SR03 (5)	SR03 (6)	SR03 (7)	SR03 (8)	SR03 (9)	SR03 (10)	SR03 (11)	SR03 (12)	SR03 (13)	SR03 (14)	SR03 (15)	SR03 (16)	SR03 (17)	SR03 (18)	SR03 (19)	SR03 (20)
Factors	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:PMM2-CDG	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT	Genotype:WT
Sorbitol	7.62E+07	7.76E+07	9.96E+07	1.08E+08	1.09E+08	9.76E+07	1.20E+08	1.23E+08	1.18E+08	9.75E+07	1.23E+08	1.26E+08	1.29E+08	9.83E+07	1.00E+08	1.10E+08	1.20E+08	1.04E+08	9.63E+07	1.23E+08	1.16E+08	1.00E+08	1.15E+08	1.23E+08	1.35E+08	8.62E+07	9.40E+07	1.04E+08	1.13E+08	9.55E+07	1.01E+08	1.15E+08	1.20E+08	21444749	31418179	38778389	34113777	31572489	35110003	34505794	31363448	32547115	42107360	31611473	26006661	28299994	34991180	33076376	35854777	35909283	43402918	55537612	2.95E+07	2.71E+07	3.67E+07	3.47E+07	1.82E+07	2.36E+07	2.35E+07	2.43E+07	2.37E+07	2.41E+07	2.41E+07	2.62E+07	3.04E+07	2.11E+07	2.69E+07	2.93E+07	1.21E+07	7.76E+06	1.63E+07	7.45E+06
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	parent formula	M/Z	HMDB ID	isotopeLabel	isotope count
Sorbitol	C6H14O6	181.07164	HMDB00247	C12 PARENT	m0-6
METABOLITES_END
#END