#METABOLOMICS WORKBENCH efahy_20230425_000040 DATATRACK_ID:3935 STUDY_ID:ST002619 ANALYSIS_ID:AN004291 PROJECT_ID:PR001020
VERSION             	1
CREATED_ON             	April 25, 2023, 7:56 am
#PROJECT
PR:PROJECT_TITLE                 	Molecular Transducer of Physical Activity Consortium
PR:PROJECT_SUMMARY               	MoTrPAC is a national research consortium designed to discover and perform
PR:PROJECT_SUMMARY               	preliminary characterization of the range of molecular transducers (the
PR:PROJECT_SUMMARY               	"molecular map") that underlie the effects of physical activity in humans. The
PR:PROJECT_SUMMARY               	program's goal is to study the molecular changes that occur during and after
PR:PROJECT_SUMMARY               	exercise and ultimately to advance the understanding of how physical activity
PR:PROJECT_SUMMARY               	improves and preserves health. Preclinical and clinical studies will examine the
PR:PROJECT_SUMMARY               	systemic effects of endurance and resistance exercise across a range of ages and
PR:PROJECT_SUMMARY               	fitness levels by molecular probing of multiple tissues before and after acute
PR:PROJECT_SUMMARY               	and chronic exercise. This program is the largest targeted NIH investment of
PR:PROJECT_SUMMARY               	funds into the mechanisms of how physical activity improves health and prevents
PR:PROJECT_SUMMARY               	disease. The MoTrPAC program is supported by the NIH Common Fund and is managed
PR:PROJECT_SUMMARY               	by a trans-agency working group representing multiple NIH institutes and
PR:PROJECT_SUMMARY               	centers, led by the NIH Office of Strategic Coordination, National Institute of
PR:PROJECT_SUMMARY               	Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes
PR:PROJECT_SUMMARY               	and Digestive and Kidney Diseases, National Institute on Aging, and National
PR:PROJECT_SUMMARY               	Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee:
PR:PROJECT_SUMMARY               	Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion
PR:PROJECT_SUMMARY               	Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac
PR:PROJECT_SUMMARY               	Bioinformatics / Data Coordination Center.
PR:INSTITUTE                     	MoTrPAC
PR:LAST_NAME                     	Ashley
PR:FIRST_NAME                    	Euan A.
PR:ADDRESS                       	300 Pasteur Dr Rm A265. MC 5319. Stanford CA 94305. USA
PR:EMAIL                         	euan@stanford.edu
PR:PHONE                         	(650) 498-4900
#STUDY
ST:STUDY_TITLE                   	MoTrPAC: Endurance exercise training study in young adult rats, Rat Brown
ST:STUDY_TITLE                   	Adipose Powder - Targeted Ethanolamides
ST:STUDY_SUMMARY                 	The goal of the endurance exercise training study in young adult rats (internal
ST:STUDY_SUMMARY                 	code: PASS1B-06) was to perform exercise training studies in adult (6 month)
ST:STUDY_SUMMARY                 	F344 rats, and from these rats collect as many tissues as feasible in order to
ST:STUDY_SUMMARY                 	provide high quality samples for detailed analysis by chemical analysis sites.
ST:STUDY_SUMMARY                 	Tissues were collected from 10-12 rats sedentary control rats concurrent with
ST:STUDY_SUMMARY                 	the collection of the 8-week training groups. The 8-week training group and
ST:STUDY_SUMMARY                 	controls were from the same cohort and same age at euthanasia (either 8). For
ST:STUDY_SUMMARY                 	the older age group, an additional set of controls (n=5-6) were collected with
ST:STUDY_SUMMARY                 	the 1-2 week training group. Rats were either sedentary or underwent an exercise
ST:STUDY_SUMMARY                 	training program. Rats were exercised on the rodent treadmill 5 days per week
ST:STUDY_SUMMARY                 	using a progressive training protocol designed to exercise the rats at
ST:STUDY_SUMMARY                 	approximately 70% of VO2max as outlined in the Table on the next page. Training
ST:STUDY_SUMMARY                 	was performed no earlier than 10:00 am and no later than 5:00 pm over 5
ST:STUDY_SUMMARY                 	consecutive days per week. Training was initiated with the treadmill set at 70%
ST:STUDY_SUMMARY                 	of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of
ST:STUDY_SUMMARY                 	exercise was increased by one minute each day until day 31 of training (start of
ST:STUDY_SUMMARY                 	week 7), when a duration of 50 min was reached. Speed and grade of each training
ST:STUDY_SUMMARY                 	session increased in larger increments due to treadmill parameters. The highest
ST:STUDY_SUMMARY                 	intensity and duration of training began on day 31. This intensity was
ST:STUDY_SUMMARY                 	maintained for the final 10 days of the protocol to ensure steady state had been
ST:STUDY_SUMMARY                 	achieved. If any rats were unable to perform at least 4 days of training per
ST:STUDY_SUMMARY                 	week they were removed from the study and euthanized. It is important to note
ST:STUDY_SUMMARY                 	that the starting treadmill speed varied depending on the sex and age of the
ST:STUDY_SUMMARY                 	rat. The initial and maximum speeds were based on VO2max measurements obtained
ST:STUDY_SUMMARY                 	during the pre-training testing of the compliant rats. Rats assigned to the
ST:STUDY_SUMMARY                 	control group followed a schedule similar to the training group. They were
ST:STUDY_SUMMARY                 	placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The
ST:STUDY_SUMMARY                 	treadmill was set at 0 m/min at an incline that corresponded to the incline
ST:STUDY_SUMMARY                 	being used by the training group.
ST:INSTITUTE                     	Emory University
ST:DEPARTMENT                    	Biochemistry
ST:LABORATORY                    	Integrated Lipidomics and Metabolomics Core
ST:LAST_NAME                     	Ortlund
ST:FIRST_NAME                    	Eric
ST:ADDRESS                       	1510 Clifton Rd. NE, Room G235, Atlanta, GA 30322
ST:EMAIL                         	eortlun@emory.edu
ST:PHONE                         	(404) 727-5014
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Rattus norvegicus
SU:TAXONOMY_ID                   	10116
#SUBJECT_SAMPLE_FACTORS:          	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	QC_prerun_1	Group:QC-PreRun | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_prerun_2	Group:QC-PreRun | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_prerun_3	Group:QC-PreRun | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_4	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_5	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_6	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_7	Group:QC-Pooled | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	0D5071F5-D166-4CF1-BE8B-EB4DF6E04C0E	90251016908	Group:Training | Timepoint:8 weeks of training or control time | Sex:Female	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	3719CB62-B551-49BE-94A0-E51DFE13D30D	90225016908	Group:Training | Timepoint:8 weeks of training or control time | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	CDCFA3F1-07ED-4737-A36D-15EFEC325AA5	90450016908	Group:Training | Timepoint:2 weeks of training | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	07AF7369-F082-4CA7-99D5-D973F4A162C9	90252016908	Group:Control | Timepoint:8 weeks of training or control time | Sex:Female	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	FCE9ABE6-B60B-4934-BAE5-56AF8B11ECEC	90239016908	Group:Control | Timepoint:8 weeks of training or control time | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	0DBFEB04-1B58-43CF-BAFD-9AA9915B67F6	90426016908	Group:Training | Timepoint:1 week of training or control time | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	0559B193-BED3-4170-B045-10A2B6441CB6	90229016908	Group:Control | Timepoint:8 weeks of training or control time | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	8904857C-2073-4BBA-AC7C-1EC239E5BFF7	90581016908	Group:Training | Timepoint:2 weeks of training | Sex:Female	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	22D700B9-EDD0-4BC8-8155-EB9C39417F81	90280016908	Group:Training | Timepoint:4 weeks of training | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	FDA87A8D-3634-4C42-9A1A-0EA03B58BB01	90449016908	Group:Training | Timepoint:2 weeks of training | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_18	Group:QC-Pooled | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	6D680EA2-C62B-4A35-8B6E-2ED6A79CB017	90421016908	Group:Training | Timepoint:1 week of training or control time | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	F9BEE1FA-E2DB-4BE4-A79C-99D585D6FD17	90265016908	Group:Control | Timepoint:8 weeks of training or control time | Sex:Female	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	A77AC89A-1D9E-4516-9DA4-2BE99C8126EF	90266016908	Group:Control | Timepoint:8 weeks of training or control time | Sex:Female	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	FE012DC0-AF32-4C70-A4D4-F042B8BC4EC6	90420016908	Group:Training | Timepoint:4 weeks of training | Sex:Female	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	1CA4210C-3CE6-4A5A-996B-673EBDEB3B81	90281016908	Group:Training | Timepoint:4 weeks of training | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	10E282EB-1DBA-406E-BD5D-81027083A01D	90430016908	Group:Training | Timepoint:1 week of training or control time | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	6527EC12-E7EB-4F17-BF0A-0AC7CD1819A0	90441016908	Group:Training | Timepoint:2 weeks of training | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	D350D633-8D95-4783-99F2-BB8294D2EC32	90423016908	Group:Training | Timepoint:1 week of training or control time | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	BC49E4EF-E2FE-4269-BAF0-461F768BD95D	90217016908	Group:Control | Timepoint:8 weeks of training or control time | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	6A013AA1-4D1E-4B75-B691-6BA22D928E51	90571016908	Group:Training | Timepoint:1 week of training or control time | Sex:Female	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_29	Group:QC-Pooled | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	B137E81D-A9DE-4E3A-8E2B-AD6C22920B7E	90576016908	Group:Training | Timepoint:2 weeks of training | Sex:Female	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	9F3E1040-D409-421E-B635-2572DC20ED89	90283016908	Group:Training | Timepoint:4 weeks of training | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	EA5C8932-9325-4DA5-96BB-50CD49CEDD10	90560016908	Group:Training | Timepoint:1 week of training or control time | Sex:Female	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	AEB1611A-F29E-43F8-B9F3-A2BC4F9EB504	90444016908	Group:Training | Timepoint:2 weeks of training | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	F0D83B29-8EFA-4CDE-B1AA-2358AC220ECF	90237016908	Group:Control | Timepoint:8 weeks of training or control time | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	569A9229-04E8-414A-A195-FE9E8F1B66AB	90289016908	Group:Training | Timepoint:4 weeks of training | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	DC8B35E8-4AA8-4829-8569-F223D069620C	90578016908	Group:Training | Timepoint:2 weeks of training | Sex:Female	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	89D6F615-D17C-4EB9-88EB-A602940FF179	90416016908	Group:Training | Timepoint:4 weeks of training | Sex:Female	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	0409BED3-F9FE-4C06-9920-74F5E5CA04A3	90587016908	Group:Training | Timepoint:2 weeks of training | Sex:Female	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	4E974304-21EB-42E8-A729-A7FB6B406E64	90439016908	Group:Training | Timepoint:2 weeks of training | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_40	Group:QC-Pooled | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	90CFE5A4-76FE-4458-A2B1-A66F0B60118E	90232016908	Group:Control | Timepoint:8 weeks of training or control time | Sex:Male	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	1F9BD644-303F-43E7-94BE-FF6654306F5E	90585016908	Group:Training | Timepoint:2 weeks of training | Sex:Female	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	68083256-8DAA-4185-B446-0CAF600474B5	90567016908	Group:Training | Timepoint:1 week of training or control time | Sex:Female	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_44	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_45	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_46	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_SRM	Group:QC-Reference | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_48	Group:QC-Pooled | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_49	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_50	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_Standard_51	Group:QC-ExternalStandard | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_Standard_52	Group:QC-ExternalStandard | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_Standard_53	Group:QC-ExternalStandard | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_Standard_54	Group:QC-ExternalStandard | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_Standard_55	Group:QC-ExternalStandard | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_Standard_56	Group:QC-ExternalStandard | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_Standard_57	Group:QC-ExternalStandard | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_Standard_58	Group:QC-ExternalStandard | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_59	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_60	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_pass1b_BAT_pos_V1-20200828.wiff
#COLLECTION
CO:SAMPLE_TYPE                   	Brown adipose
#TREATMENT
TR:TREATMENT_SUMMARY             	-
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	purification of oxylipids with SPE column
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Lipids were separated by liquid chromatography with 20 minutes gradient
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	ExionLC AC
CH:COLUMN_NAME                   	ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um)
CH:SOLVENT_A                     	100% water; 10 mM ammonium acetate
CH:SOLVENT_B                     	100% acetonitrile; 10 mM ammonium acetate
CH:FLOW_GRADIENT                 	t=0 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=0.5 min: 90%/10% (%A/%B) @ 0.5 ml/min
CH:FLOW_GRADIENT                 	| t=1 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2 min: 50%/50% (%A/%B) @ 0.5 ml/min
CH:FLOW_GRADIENT                 	| t=2.1 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=5 min: 25%/75% (%A/%B) @ 0.5
CH:FLOW_GRADIENT                 	ml/min | t=7 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12 min: 15%/85% (%A/%B) @ 0.5
CH:FLOW_GRADIENT                 	ml/min | t=12.1 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=18 min: 90%/10% (%A/%B) @
CH:FLOW_GRADIENT                 	0.5 ml/min
CH:FLOW_RATE                     	0.5 ml/min
CH:COLUMN_TEMPERATURE            	50C
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:ANALYSIS_DETAILS:             	Peak integration, concentrations calculated base on standards curves equations,
AN:ANALYSIS_DETAILS:             	in the result file data represented in ng/mg of tissue
#MS
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:INSTRUMENT_NAME               	ABI Sciex 5500 Qtrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	Multiple reaction monitoring mode (MRM)
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	ng/uL
MS_METABOLITE_DATA_START
Samples	QC_prerun_1	QC_prerun_2	QC_prerun_3	QC_blank_4	QC_blank_5	QC_blank_6	QC_7	90251016908	90225016908	90450016908	90252016908	90239016908	90426016908	90229016908	90581016908	90280016908	90449016908	QC_18	90421016908	90265016908	90266016908	90420016908	90281016908	90430016908	90441016908	90423016908	90217016908	90571016908	QC_29	90576016908	90283016908	90560016908	90444016908	90237016908	90289016908	90578016908	90416016908	90587016908	90439016908	QC_40	90232016908	90585016908	90567016908	QC_blank_44	QC_blank_45	QC_blank_46	QC_SRM	QC_48	QC_blank_49	QC_blank_50	QC_Standard_51	QC_Standard_52	QC_Standard_53	QC_Standard_54	QC_Standard_55	QC_Standard_56	QC_Standard_57	QC_Standard_58	QC_blank_59	QC_blank_60
Linoleoyl-EA	0.006735252	0.006301788	0.006941875	4.38503e-05	6.62568e-05	4.8076e-05	0.04011474	0.004902037	0.004363569	0.004307574	0.009899537	0.003535122	0.002708917	0.002205786	0.00633022	0.021479904	0.006117332	0.010769172	0.01102802	0.00850469	0.006578658	0.008271576	0.006559961	0.00821269	0.008639401	0.005209626	0.006554646	0.006480227	0.008271539	0.012705415	0.005309369	0.008350787	0.004070949	0.00491114	0.010577602	0.022739609	0.011788521	0.007836523	0.010970615	0.041526003	0.003795584	0.010938399	0.010386645	6.43293e-05	2.64245e-05	3.618e-05	0.002019904	0.022184031	3.23608e-05	0.000454226	0.172601744	0.345203488	0.690406977	1.380813953	2.761627907	5.523255814	11.04651163	22.09302326	0.001113929	0.000999849
Oleoyl-EA	0	0	0	0	0	0	0.002293031	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	9.73838e-05	0	0	0	0	0	0.001047604	0	0	0	0.001748474	0	0	0	0	0	0	0	0.000166928	0	0	0.016351744	0.032703488	0.065406977	0.130813953	0.261627907	0.523255814	1.046511628	2.093023256	0	0
Arachidonoyl-EA	0.000262316	0.00025267	0.000267504	4.55838e-05	5.21968e-05	3.77443e-05	0.001736804	0.000417778	0.000344907	0.000451215	0.000805007	0.000347256	0.000256801	0.000212194	0.000753163	0.001739756	0.000691821	0.00048116	0.000975552	0.000748106	0.000765948	0.000688485	0.000436024	0.000984515	0.000952149	0.000584242	0.000473973	0.000534133	0.000344081	0.000995491	0.000579462	0.000888618	0.000533522	0.000407583	0.00118145	0.003076193	0.000989334	0.00077757	0.00110576	0.001726336	0.000260847	0.000958651	0.000951049	2.96348e-05	2.2175e-05	2.4875e-05	0.000461704	0.000926583	1.7825e-05	0.000323251	0.163517442	0.327034884	0.654069767	1.308139535	2.61627907	5.23255814	10.46511628	20.93023256	0.000987307	0.000877637
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	formula	mz	neutral_mass	refmet_name	rt
Oleoyl-EA	C20H39NO2	326.4	325.2981	Oleoyl-EA	6.7
Arachidonoyl-EA	C22H37NO2	348.2	347.2824	Arachidonoyl-EA	5.59
Linoleoyl-EA	C20H37NO2	324.3	323.2824	Linoleoyl-EA	5.66
METABOLITES_END
#END