#METABOLOMICS WORKBENCH ijochmans_20230717_034505 DATATRACK_ID:4168 STUDY_ID:ST002784 ANALYSIS_ID:AN004531 PROJECT_ID:PR001736 VERSION 1 CREATED_ON July 18, 2023, 9:41 am #PROJECT PR:PROJECT_TITLE Metabolic changes during normothermic isolated kidney perfusion PR:PROJECT_TYPE Experimental PR:PROJECT_SUMMARY Normothermic isolated kidney perfusion is being developed as a method to PR:PROJECT_SUMMARY preserve donor kidneys and to assess their future function before they are PR:PROJECT_SUMMARY transplanted. Donor kidneys can be exposed to different types and degrees of PR:PROJECT_SUMMARY ischemic injury before they are preserved. This project studies how the PR:PROJECT_SUMMARY metabolome changes in relation to different types and degrees of ischemia. PR:INSTITUTE KU Leuven PR:DEPARTMENT Microbiology, Immunology and Transplantation PR:LABORATORY Lab of Abdominal Transplantation PR:LAST_NAME Jochmans PR:FIRST_NAME Ina PR:ADDRESS Herestraat 49, Leuven, 3000, Belgium PR:EMAIL ina.jochmans@kuleuven.be PR:PHONE None PR:FUNDING_SOURCE FTBO, KOOR, FWO PR:CONTRIBUTORS Julie De Beule, Bart Ghesquière, Ina Jochmans #STUDY ST:STUDY_TITLE Metabolic changes of the pig kidney during isolated normothermic perfusion with ST:STUDY_TITLE red blood cell based perfusate - perfusate ST:STUDY_SUMMARY This study investigates how glucose, lactate and 20 amino acids in the perfusate ST:STUDY_SUMMARY of normothermically perfused pig kidneys changes over time. It also studies how ST:STUDY_SUMMARY type and severity of ischemia before perfusion (cold or warm ischemia) change ST:STUDY_SUMMARY this profile. ST:INSTITUTE KU Leuven ST:DEPARTMENT Microbiology, Immunology and Transplantation ST:LABORATORY Lab of Abdominal Transplantation ST:LAST_NAME Jochmans ST:FIRST_NAME Ina ST:ADDRESS Herestraat 49, Leuven, 3000, Belgium ST:EMAIL ina.jochmans@kuleuven.be ST:NUM_GROUPS 3 ST:TOTAL_SUBJECTS 10 kidneys ST:NUM_MALES 5 male pigs ST:STUDY_TYPE Experimental ST:PHONE None #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Sus scrofa SU:TAXONOMY_ID 9823 SU:AGE_OR_AGE_RANGE ~4 months SU:WEIGHT_OR_WEIGHT_RANGE 35-45 kg SU:GENDER Male SU:ANIMAL_ANIMAL_SUPPLIER TOPIGS TN70, Tojapigs, the Netherlands SU:ANIMAL_LIGHT_CYCLE 12 hours SU:ANIMAL_WATER ad libitum #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS 45 45_T0 Group:WI | Timepoint:0 RAW_FILE_NAME=45_T0.mzML SUBJECT_SAMPLE_FACTORS 45 45_T0.25 Group:WI | Timepoint:15 RAW_FILE_NAME=45_T0.25.mzML SUBJECT_SAMPLE_FACTORS 45 45_T0.50 Group:WI | Timepoint:30 RAW_FILE_NAME=45_T0.5.mzML SUBJECT_SAMPLE_FACTORS 45 45_T0.75 Group:WI | Timepoint:45 RAW_FILE_NAME=45_T0.75.mzML SUBJECT_SAMPLE_FACTORS 45 45_T1 Group:WI | Timepoint:60 RAW_FILE_NAME=45_T1.mzML SUBJECT_SAMPLE_FACTORS 45 45_T2 Group:WI | Timepoint:120 RAW_FILE_NAME=45_T2.mzML SUBJECT_SAMPLE_FACTORS 45 45_T3 Group:WI | Timepoint:180 RAW_FILE_NAME=45_T3.mzML SUBJECT_SAMPLE_FACTORS 45 45_T4 Group:WI | Timepoint:240 RAW_FILE_NAME=45_T4.mzML SUBJECT_SAMPLE_FACTORS 46 46_T0 Group:WI | Timepoint:0 RAW_FILE_NAME=46_T0.mzML SUBJECT_SAMPLE_FACTORS 46 46_T0.25 Group:WI | Timepoint:15 RAW_FILE_NAME=46_T0.25.mzML SUBJECT_SAMPLE_FACTORS 46 46_T0.50 Group:WI | Timepoint:30 RAW_FILE_NAME=46_T0.5.mzML SUBJECT_SAMPLE_FACTORS 46 46_T0.75 Group:WI | Timepoint:45 RAW_FILE_NAME=46_T0.75.mzML SUBJECT_SAMPLE_FACTORS 46 46_T1 Group:WI | Timepoint:60 RAW_FILE_NAME=46_T1.mzML SUBJECT_SAMPLE_FACTORS 46 46_T2 Group:WI | Timepoint:120 RAW_FILE_NAME=46_T2.mzML SUBJECT_SAMPLE_FACTORS 46 46_T3 Group:WI | Timepoint:180 RAW_FILE_NAME=46_T3.mzML SUBJECT_SAMPLE_FACTORS 46 46_T4 Group:WI | Timepoint:240 RAW_FILE_NAME=46_T4.mzML SUBJECT_SAMPLE_FACTORS 47 47_T0 Group:Control | Timepoint:0 RAW_FILE_NAME=47_T0.mzML SUBJECT_SAMPLE_FACTORS 47 47_T0.25 Group:Control | Timepoint:15 RAW_FILE_NAME=47_T0.25.mzML SUBJECT_SAMPLE_FACTORS 47 47_T0.50 Group:Control | Timepoint:30 RAW_FILE_NAME=47_T0.5.mzML SUBJECT_SAMPLE_FACTORS 47 47_T0.75 Group:Control | Timepoint:45 RAW_FILE_NAME=47_T0.75.mzML SUBJECT_SAMPLE_FACTORS 47 47_T1 Group:Control | Timepoint:60 RAW_FILE_NAME=47_T1.mzML SUBJECT_SAMPLE_FACTORS 47 47_T2 Group:Control | Timepoint:120 RAW_FILE_NAME=47_T2.mzML SUBJECT_SAMPLE_FACTORS 47 47_T3 Group:Control | Timepoint:180 RAW_FILE_NAME=47_T3.mzML SUBJECT_SAMPLE_FACTORS 47 47_T4 Group:Control | Timepoint:240 RAW_FILE_NAME=47_T4.mzML SUBJECT_SAMPLE_FACTORS 48 48_T0 Group:CI | Timepoint:0 RAW_FILE_NAME=48_T0.mzML SUBJECT_SAMPLE_FACTORS 48 48_T0.25 Group:CI | Timepoint:15 RAW_FILE_NAME=48_T0.25.mzML SUBJECT_SAMPLE_FACTORS 48 48_T0.50 Group:CI | Timepoint:30 RAW_FILE_NAME=48_T0.5.mzML SUBJECT_SAMPLE_FACTORS 48 48_T0.75 Group:CI | Timepoint:45 RAW_FILE_NAME=48_T0.75.mzML SUBJECT_SAMPLE_FACTORS 48 48_T1 Group:CI | Timepoint:60 RAW_FILE_NAME=48_T1.mzML SUBJECT_SAMPLE_FACTORS 48 48_T2 Group:CI | Timepoint:120 RAW_FILE_NAME=48_T2.mzML SUBJECT_SAMPLE_FACTORS 48 48_T3 Group:CI | Timepoint:180 RAW_FILE_NAME=48_T3.mzML SUBJECT_SAMPLE_FACTORS 48 48_T4 Group:CI | Timepoint:240 RAW_FILE_NAME=48_T4.mzML SUBJECT_SAMPLE_FACTORS 49 49_T0 Group:Control | Timepoint:0 RAW_FILE_NAME=49_T0.mzML SUBJECT_SAMPLE_FACTORS 49 49_T0.25 Group:Control | Timepoint:15 RAW_FILE_NAME=49_T0.25.mzML SUBJECT_SAMPLE_FACTORS 49 49_T0.50 Group:Control | Timepoint:30 RAW_FILE_NAME=49_T0.5.mzML SUBJECT_SAMPLE_FACTORS 49 49_T0.75 Group:Control | Timepoint:45 RAW_FILE_NAME=49_T0.75.mzML SUBJECT_SAMPLE_FACTORS 49 49_T1 Group:Control | Timepoint:60 RAW_FILE_NAME=49_T1.mzML SUBJECT_SAMPLE_FACTORS 49 49_T2 Group:Control | Timepoint:120 RAW_FILE_NAME=49_T2.mzML SUBJECT_SAMPLE_FACTORS 49 49_T3 Group:Control | Timepoint:180 RAW_FILE_NAME=49_T3.mzML SUBJECT_SAMPLE_FACTORS 49 49_T4 Group:Control | Timepoint:240 RAW_FILE_NAME=49_T4.mzML SUBJECT_SAMPLE_FACTORS 50 50_T0 Group:CI | Timepoint:0 RAW_FILE_NAME=50_T0.mzML SUBJECT_SAMPLE_FACTORS 50 50_T0.25 Group:CI | Timepoint:15 RAW_FILE_NAME=50_T0.25.mzML SUBJECT_SAMPLE_FACTORS 50 50_T0.50 Group:CI | Timepoint:30 RAW_FILE_NAME=50_T0.5.mzML SUBJECT_SAMPLE_FACTORS 50 50_T0.75 Group:CI | Timepoint:45 RAW_FILE_NAME=50_T0.75.mzML SUBJECT_SAMPLE_FACTORS 50 50_T1 Group:CI | Timepoint:60 RAW_FILE_NAME=50_T1.mzML SUBJECT_SAMPLE_FACTORS 50 50_T2 Group:CI | Timepoint:120 RAW_FILE_NAME=50_T2.mzML SUBJECT_SAMPLE_FACTORS 50 50_T3 Group:CI | Timepoint:180 RAW_FILE_NAME=50_T3.mzML SUBJECT_SAMPLE_FACTORS 50 50_T4 Group:CI | Timepoint:240 RAW_FILE_NAME=50_T4.mzML SUBJECT_SAMPLE_FACTORS 51 51_T0 Group:Control | Timepoint:0 RAW_FILE_NAME=51_T0.mzML SUBJECT_SAMPLE_FACTORS 51 51_T0.25 Group:Control | Timepoint:15 RAW_FILE_NAME=51_T0.25.mzML SUBJECT_SAMPLE_FACTORS 51 51_T0.50 Group:Control | Timepoint:30 RAW_FILE_NAME=51_T0.5.mzML SUBJECT_SAMPLE_FACTORS 51 51_T0.75 Group:Control | Timepoint:45 RAW_FILE_NAME=51_T0.75.mzML SUBJECT_SAMPLE_FACTORS 51 51_T1 Group:Control | Timepoint:60 RAW_FILE_NAME=51_T1.mzML SUBJECT_SAMPLE_FACTORS 51 51_T2 Group:Control | Timepoint:120 RAW_FILE_NAME=51_T2.mzML SUBJECT_SAMPLE_FACTORS 51 51_T3 Group:Control | Timepoint:180 RAW_FILE_NAME=51_T3.mzML SUBJECT_SAMPLE_FACTORS 51 51_T4 Group:Control | Timepoint:240 RAW_FILE_NAME=51_T4.mzML SUBJECT_SAMPLE_FACTORS 52 52_T0 Group:CI | Timepoint:0 RAW_FILE_NAME=52_T0.mzML SUBJECT_SAMPLE_FACTORS 52 52_T0.25 Group:CI | Timepoint:15 RAW_FILE_NAME=52_T0.25.mzML SUBJECT_SAMPLE_FACTORS 52 52_T0.50 Group:CI | Timepoint:30 RAW_FILE_NAME=52_T0.5.mzML SUBJECT_SAMPLE_FACTORS 52 52_T0.75 Group:CI | Timepoint:45 RAW_FILE_NAME=52_T0.75.mzML SUBJECT_SAMPLE_FACTORS 52 52_T1 Group:CI | Timepoint:60 RAW_FILE_NAME=52_T1.mzML SUBJECT_SAMPLE_FACTORS 52 52_T2 Group:CI | Timepoint:120 RAW_FILE_NAME=52_T2.mzML SUBJECT_SAMPLE_FACTORS 52 52_T3 Group:CI | Timepoint:180 RAW_FILE_NAME=52_T3.mzML SUBJECT_SAMPLE_FACTORS 52 52_T4 Group:CI | Timepoint:240 RAW_FILE_NAME=52_T4.mzML SUBJECT_SAMPLE_FACTORS 53 53_T0 Group:WI | Timepoint:0 RAW_FILE_NAME=53_T0.mzML SUBJECT_SAMPLE_FACTORS 53 53_T0.25 Group:WI | Timepoint:15 RAW_FILE_NAME=53_T0.25.mzML SUBJECT_SAMPLE_FACTORS 53 53_T0.50 Group:WI | Timepoint:30 RAW_FILE_NAME=53_T0.5.mzML SUBJECT_SAMPLE_FACTORS 53 53_T0.75 Group:WI | Timepoint:45 RAW_FILE_NAME=53_T0.75.mzML SUBJECT_SAMPLE_FACTORS 53 53_T1 Group:WI | Timepoint:60 RAW_FILE_NAME=53_T1.mzML SUBJECT_SAMPLE_FACTORS 53 53_T2 Group:WI | Timepoint:120 RAW_FILE_NAME=53_T2.mzML SUBJECT_SAMPLE_FACTORS 53 53_T3 Group:WI | Timepoint:180 RAW_FILE_NAME=53_T3.mzML SUBJECT_SAMPLE_FACTORS 53 53_T4 Group:WI | Timepoint:240 RAW_FILE_NAME=53_T4.mzML SUBJECT_SAMPLE_FACTORS 54 54_T0 Group:CI | Timepoint:0 RAW_FILE_NAME=54_T0.mzML SUBJECT_SAMPLE_FACTORS 54 54_T0.25 Group:CI | Timepoint:15 RAW_FILE_NAME=54_T0.25.mzML SUBJECT_SAMPLE_FACTORS 54 54_T0.50 Group:CI | Timepoint:30 RAW_FILE_NAME=54_T0.5.mzML SUBJECT_SAMPLE_FACTORS 54 54_T0.75 Group:CI | Timepoint:45 RAW_FILE_NAME=54_T0.75.mzML SUBJECT_SAMPLE_FACTORS 54 54_T1 Group:CI | Timepoint:60 RAW_FILE_NAME=54_T1.mzML SUBJECT_SAMPLE_FACTORS 54 54_T2 Group:CI | Timepoint:120 RAW_FILE_NAME=54_T2.mzML SUBJECT_SAMPLE_FACTORS 54 54_T3 Group:CI | Timepoint:180 RAW_FILE_NAME=54_T3.mzML SUBJECT_SAMPLE_FACTORS 54 54_T4 Group:CI | Timepoint:240 RAW_FILE_NAME=54_T4.mzML #COLLECTION CO:COLLECTION_SUMMARY Perfusate samples were collected in ethylenediaminetetraacetic acid (EDTA) CO:COLLECTION_SUMMARY tubes, at baseline (before the kidney was mounted on the normothermic perfusion CO:COLLECTION_SUMMARY device), every 15 minutes during the first hour and hourly thereafter. One CO:COLLECTION_SUMMARY milliliter perfusate aliquots (centrifuged 1000g, 10 minutes, 4°C) were snap CO:COLLECTION_SUMMARY frozen in liquid nitrogen and stored at -80°C until analysis. CO:SAMPLE_TYPE Perfusate CO:COLLECTION_FREQUENCY Baseline (T0) and during perfusion at 15 min (T0.25), 30 min (T0.50), 45 min CO:COLLECTION_FREQUENCY (T0.75), 1 hour (T1), 2 hours (T2), 3 hours (T3), 4 hours (T4) CO:STORAGE_CONDITIONS -80℃ CO:ADDITIVES EDTA #TREATMENT TR:TREATMENT_SUMMARY Three experimental conditions were investigated (n=3/group for Control and WI, TR:TREATMENT_SUMMARY n=4/group for CI): (a) Controls; (b) warm ischemia (WI) simulating hypoxic acute TR:TREATMENT_SUMMARY kidney injury; (c) cold ischemia (CI) replicating clinical cold storage. Control TR:TREATMENT_SUMMARY kidneys were retrieved, flushed with cold IGL-1, and immediately reperfused. CI TR:TREATMENT_SUMMARY group, kidneys were exposed to 22h of CI by submerging them in IGL- 1 (a TR:TREATMENT_SUMMARY clinical preservation solution for cold storage) and storing them on ice. In WI, TR:TREATMENT_SUMMARY the renal artery and vein were clamped for 60 min before retrieval. All kidneys TR:TREATMENT_SUMMARY were flushed with 200 ml of Ringer’s solution before mounting them on the ex TR:TREATMENT_SUMMARY situ circuit to wash out IGL-1. TR:ANIMAL_ANESTHESIA Pigs were sedated by an intramuscular injection of Tiletamine/Zolazepam (8 TR:ANIMAL_ANESTHESIA mg/kg, Zoletil®, Virbac, Belgium) and Xylazine (2 mg/kg, Xylazine®, VMD TR:ANIMAL_ANESTHESIA pharma, Belgium) to allow orotracheal intubation. Anesthesia was maintained by TR:ANIMAL_ANESTHESIA inhalation of isoflurane (1% Isovet®, Piramal Critical Care B.V., Belgium) and TR:ANIMAL_ANESTHESIA continuous infusion of fentanyl (8 µg/kg, Fentanyl®, Janssen Pharmaceutica, TR:ANIMAL_ANESTHESIA Belgium). TR:ANIMAL_ACCLIMATION_DURATION Minimum of 2 days TR:ANIMAL_FASTING Overnight TR:ANIMAL_ENDP_EUTHANASIA Non-recovery study #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Samples were extracted in an 80% methanol (80:20 methanol:water) (Methanol SP:SAMPLEPREP_SUMMARY ≥99.9%, HiPerSolv CHROMANORM®, ULTRA for LC-MS, suitable for UPLC/UHPLC-MS SP:SAMPLEPREP_SUMMARY instruments, VWR, Belgium) extraction buffer containing 1 µM of deuterated D27 SP:SAMPLEPREP_SUMMARY myristic acid, 5 µM D12 glucose, 3 µM 13C5-D5-15N Glutamic acid and 3 µM SP:SAMPLEPREP_SUMMARY D7-15N4-Arginine as internal standards. 10 µl of sample was added to 990 µl of SP:SAMPLEPREP_SUMMARY the extraction buffer and stored overnight at -80 °C. Insolubilities and SP:SAMPLEPREP_SUMMARY precipitated proteins were removed by centrifugation at 20.000 g, for 15 min at SP:SAMPLEPREP_SUMMARY 4 °C. 200 µL of the supernatant was transferred to an appropriate SP:SAMPLEPREP_SUMMARY mass-spectrometry vial. 10 µl of sample was added to 990 µl of the extraction SP:SAMPLEPREP_SUMMARY buffer and stored overnight at -80 °C. SP:EXTRACT_STORAGE -80℃ #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY 10 µl was loaded onto an Ultra Performance Liquid Chromatograph (UPLC) equipped CH:CHROMATOGRAPHY_SUMMARY with a Hydrophilic Interaction Liquid Chromatography (HILIC) column (InfinityLab CH:CHROMATOGRAPHY_SUMMARY Poroshell 120 HILIC-Z PEEK-lined 2.1 x 150 mm, 2.7 µm column; Agilent, Santa CH:CHROMATOGRAPHY_SUMMARY Clara, USA)) and connected in-line to a Q-exactive Orbitrap Focus (Thermo Fisher CH:CHROMATOGRAPHY_SUMMARY Scientific, Massachusetts, USA) mass spectrometer. A linear gradient was built CH:CHROMATOGRAPHY_SUMMARY up starting with 90% solvent A (LC-MS grade acetonitrile, Acetonitrile CH:CHROMATOGRAPHY_SUMMARY hypergrade for LC-MS LiChrosolv, Supelco (Merck), Germany) and 10% solvent B (10 CH:CHROMATOGRAPHY_SUMMARY mM ammonium acetate (LiChropur™, eluent additive for LC-MS, (Merck), Germany), CH:CHROMATOGRAPHY_SUMMARY pH 9.3). At 2 min the gradient increased to 60% of solvent B and maintained at CH:CHROMATOGRAPHY_SUMMARY 60% until 15 min. The gradient returned to 10% solvent B at 16 min and remained CH:CHROMATOGRAPHY_SUMMARY until 25 min. The flow rate was 250 µl/min and the column was kept at 25°C CH:CHROMATOGRAPHY_SUMMARY throughout the analysis. The MS operated in negative ion mode, with a spray CH:CHROMATOGRAPHY_SUMMARY voltage of 2.9 kV and a temperature of the capillary of 325 °C. Gas settings CH:CHROMATOGRAPHY_SUMMARY were as follows: sheath gas 40 and auxiliary gas 15. The vaporizer temperature CH:CHROMATOGRAPHY_SUMMARY was set at 300 °C. A full scan (resolution of 70.000 and scan range of m/z CH:CHROMATOGRAPHY_SUMMARY 70-1050) was applied. XCalibur version to operate the LC-MS was 4.2.47. CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Agilent 1290 Infinity CH:COLUMN_NAME Agilent InfinityLab Poroshell 120 (2.1 x 150 mm, 2.7 um) CH:SOLVENT_A 100% acetonitrile CH:SOLVENT_B 100% water; 10 mM ammonium acetate, pH 9.3 CH:FLOW_GRADIENT A linear gradient was built up starting with 90% solvent A and 10% solvent B. At CH:FLOW_GRADIENT 2 min the gradient increased to 60% of solvent B and maintained at 60% until 15 CH:FLOW_GRADIENT min. CH:FLOW_RATE 250 µl/min CH:COLUMN_TEMPERATURE 25°C #ANALYSIS AN:ANALYSIS_TYPE MS AN:LABORATORY_NAME Laboratory of Applied Mass Spectrometry, KU Leuven #MS MS:INSTRUMENT_NAME Thermo Q Exactive Focus MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS Raw MS data were converted into mzML using the MSConvert tool of Proteowizard MS:MS_COMMENTS (version 3.0.20247). Peak picking was performed with El_Maven (El_Maven, MS:MS_COMMENTS Elucidata, Massachusetts, USA, version 0.12.0). Metabolites were identified MS:MS_COMMENTS using an in-house library containing exact mass and retention time. The mass MS:MS_COMMENTS accuracy during data processing in El Maven was set at 10 ppm. Calculation of MS:MS_COMMENTS abundances was done in the LC-MS Workflow of El_Maven. Raw abundances (peak area MS:MS_COMMENTS values) for each metabolite were corrected for internal standard (Myristic acid MS:MS_COMMENTS d27). #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Peak area MS_METABOLITE_DATA_START Samples 45_T0 45_T0.25 45_T0.50 45_T0.75 45_T1 45_T2 45_T3 45_T4 46_T0 46_T0.25 46_T0.50 46_T0.75 46_T1 46_T2 46_T3 46_T4 47_T0 47_T0.25 47_T0.50 47_T0.75 47_T1 47_T2 47_T3 47_T4 48_T0 48_T0.25 48_T0.50 48_T0.75 48_T1 48_T2 48_T3 48_T4 49_T0 49_T0.25 49_T0.50 49_T0.75 49_T1 49_T2 49_T3 49_T4 50_T0 50_T0.25 50_T0.50 50_T0.75 50_T1 50_T2 50_T3 50_T4 51_T0 51_T0.25 51_T0.50 51_T0.75 51_T1 51_T2 51_T3 51_T4 52_T0 52_T0.25 52_T0.50 52_T0.75 52_T1 52_T2 52_T3 52_T4 53_T0 53_T0.25 53_T0.50 53_T0.75 53_T1 53_T2 53_T3 53_T4 54_T0 54_T0.25 54_T0.50 54_T0.75 54_T1 54_T2 54_T3 54_T4 Factors Group:WI | Timepoint:0 Group:WI | Timepoint:15 Group:WI | Timepoint:30 Group:WI | Timepoint:45 Group:WI | Timepoint:60 Group:WI | Timepoint:120 Group:WI | Timepoint:180 Group:WI | Timepoint:240 Group:WI | Timepoint:0 Group:WI | Timepoint:15 Group:WI | Timepoint:30 Group:WI | Timepoint:45 Group:WI | Timepoint:60 Group:WI | Timepoint:120 Group:WI | Timepoint:180 Group:WI | Timepoint:240 Group:Control | Timepoint:0 Group:Control | Timepoint:15 Group:Control | Timepoint:30 Group:Control | Timepoint:45 Group:Control | Timepoint:60 Group:Control | Timepoint:120 Group:Control | Timepoint:180 Group:Control | Timepoint:240 Group:CI | Timepoint:0 Group:CI | Timepoint:15 Group:CI | Timepoint:30 Group:CI | Timepoint:45 Group:CI | Timepoint:60 Group:CI | Timepoint:120 Group:CI | Timepoint:180 Group:CI | Timepoint:240 Group:Control | Timepoint:0 Group:Control | Timepoint:15 Group:Control | Timepoint:30 Group:Control | Timepoint:45 Group:Control | Timepoint:60 Group:Control | Timepoint:120 Group:Control | Timepoint:180 Group:Control | Timepoint:240 Group:CI | Timepoint:0 Group:CI | Timepoint:15 Group:CI | Timepoint:30 Group:CI | Timepoint:45 Group:CI | Timepoint:60 Group:CI | Timepoint:120 Group:CI | Timepoint:180 Group:CI | Timepoint:240 Group:Control | Timepoint:0 Group:Control | Timepoint:15 Group:Control | Timepoint:30 Group:Control | Timepoint:45 Group:Control | Timepoint:60 Group:Control | Timepoint:120 Group:Control | Timepoint:180 Group:Control | Timepoint:240 Group:CI | Timepoint:0 Group:CI | Timepoint:15 Group:CI | Timepoint:30 Group:CI | Timepoint:45 Group:CI | Timepoint:60 Group:CI | Timepoint:120 Group:CI | Timepoint:180 Group:CI | Timepoint:240 Group:WI | Timepoint:0 Group:WI | Timepoint:15 Group:WI | Timepoint:30 Group:WI | Timepoint:45 Group:WI | Timepoint:60 Group:WI | Timepoint:120 Group:WI | Timepoint:180 Group:WI | Timepoint:240 Group:CI | Timepoint:0 Group:CI | Timepoint:15 Group:CI | Timepoint:30 Group:CI | Timepoint:45 Group:CI | Timepoint:60 Group:CI | Timepoint:120 Group:CI | Timepoint:180 Group:CI | Timepoint:240 Glucose 0.972130504 0.90725567 0.839527841 0.900205733 0.870442298 0.901622335 0.831081399 0.726023453 0.989919535 0.980165421 0.960730742 0.938109226 0.882087525 0.910609846 0.820062648 0.821213724 0.991850041 1.085308132 1.169338221 1.182325357 1.292854484 1.33692296 1.116452637 0.900065221 0.850544413 0.930119491 0.876447022 1.039902887 1.059069818 1.052423114 0.950265048 0.839671291 1.055331912 0.855154061 1.055564373 1.161424156 1.195670261 1.241712389 1.116352193 0.854806805 0.95739373 0.956989333 0.953773149 0.965942538 0.974134435 0.82420111 0.606361991 0.390171353 0.88885313 0.931160483 1.004274288 1.016630994 1.122755272 1.202514396 1.034995869 0.817235848 0.950825417 0.817325856 0.928415829 0.928793166 1.07689566 1.044078815 0.787866263 0.623983754 1.058621892 0.908079528 0.874007282 0.906045263 0.859388956 0.821301312 0.819569781 0.726287156 0.88450293 0.878090579 0.936043116 1.000637307 1.029958439 1.145507792 0.894562278 0.6271467 Lactate 1.048975724 4.171060757 4.513973217 4.902988021 6.353255578 9.78449507 11.93618804 15.75455143 1.020704015 2.848894099 3.678329275 4.91499345 5.887235537 9.977985151 11.84286369 15.33998009 0.875436025 1.62829289 1.416961449 1.0860756 1.173832739 3.597575837 6.771212433 7.932106708 1.963669027 3.464578946 2.739730835 2.635734718 2.060844719 3.612614482 8.419044041 13.25973825 0.998545854 1.325976491 1.102328186 1.164816042 1.330596091 3.108805607 6.71037726 8.794800963 1.634491343 3.222655345 2.569040344 2.128513904 1.765915446 4.285938212 7.615423425 10.63318487 0.804409429 1.283531933 1.021876154 0.579789462 0.594905539 2.346402902 6.13619111 10.65408649 1.64450738 4.698522114 5.648383306 4.900360042 4.56193407 6.620309835 12.2790578 18.52895723 0.995625726 3.166495821 4.531724442 5.973029943 6.490411363 8.746057541 12.44238785 17.7659014 1.455403513 2.427617135 2.903212605 2.892335151 2.514934693 4.119648368 10.18544629 15.92349567 Gly 0.004844811 0.015807829 0.016625703 0.017407257 0.021263836 0.030779981 0.034284526 0.035585506 0.004886446 0.011118539 0.013998278 0.018233187 0.020247961 0.029341325 0.03151871 0.035002337 0.00485408 0.024209908 0.03122574 0.036169645 0.039722582 0.043669361 0.043948658 0.044555706 0.004881381 0.018523929 0.021118142 0.025126301 0.025443813 0.027849518 0.032858742 0.038223998 0.005120277 0.015869334 0.024793168 0.030515515 0.034268886 0.043667 0.048004449 0.050417924 0.005018262 0.017357517 0.020627312 0.023549049 0.024438386 0.026850129 0.029649096 0.033535518 0.004882272 0.019540103 0.02596116 0.027583872 0.033403974 0.046503312 0.048575411 0.052220672 0.005003422 0.016022463 0.019742173 0.019568004 0.022880036 0.027204965 0.030482441 0.034072327 0.004864459 0.013439548 0.02084 0.027034602 0.028992569 0.034795206 0.039075186 0.041504697 0.004845624 0.015128116 0.020381107 0.024295497 0.025458154 0.030080923 0.031351634 0.034569415 Gln 0.920910532 0.561860733 0.581581451 0.624098467 0.563103018 0.409977426 0.267447476 0.264141239 0.928260888 0.730951666 0.705357904 0.662591357 0.626289141 0.567154158 0.486900642 0.489408502 0.970785076 0.747800409 0.677686863 0.562060153 0.515760063 0.093248221 0.05513972 0.061474058 1.002088627 0.668147329 0.499716036 0.448100696 0.344118537 0.177508781 0.139672057 0.107675558 1.046430019 0.477658407 0.461270999 0.455957013 0.409232718 0.217332887 0.057521278 0.074621165 0.907599076 0.427472465 0.396951566 0.316855096 0.305781171 0.106176974 0.102042357 0.083105656 0.954046411 0.637713343 0.6659882 0.609132946 0.57690567 0.407025454 0.152878005 0.105307697 0.901471577 0.547469555 0.520125738 0.452863393 0.453030338 0.385751577 0.360463158 0.359421349 1.005659027 0.657082849 0.52651981 0.444581159 0.370866927 0.197318066 0.141856339 0.168172926 0.933082943 0.504233835 0.40352543 0.311918592 0.276203773 0.154667411 0.126732936 0.141188266 Lys 0.011558497 0.012527708 0.014036236 0.014853449 0.015969818 0.028006202 0.038273862 0.043762283 0.011657828 0.01210223 0.0122104 0.014117888 0.013749128 0.02157097 0.026100241 0.03136409 0.011580612 0.013501716 0.022017984 0.029643308 0.036176051 0.065655795 0.080841842 0.087736953 0.011645745 0.014510538 0.019676717 0.02525032 0.024873759 0.032920205 0.041772628 0.052454736 0.01221569 0.016492899 0.019751318 0.02913348 0.034765574 0.058459622 0.07491397 0.084524734 0.011972308 0.013809171 0.01897988 0.024142768 0.025026891 0.036585662 0.048090258 0.057292339 0.011647871 0.012227383 0.02108574 0.030403597 0.037072921 0.06006205 0.073315778 0.082683696 0.011936905 0.012691931 0.018172139 0.022423921 0.026486657 0.036463024 0.041202375 0.048206578 0.011605375 0.011695256 0.011564293 0.014700793 0.015807867 0.022807077 0.029159374 0.033564847 0.011560438 0.012037919 0.016306922 0.020879575 0.021743268 0.032024433 0.036467567 0.04273937 Met 0.001205535 0.009667126 0.010511092 0.011706643 0.016297901 0.03354288 0.047668718 0.054940979 0.000854503 0.006513919 0.008831747 0.012457628 0.014301647 0.026975173 0.035050898 0.044062884 0.000734864 0.012104465 0.019919097 0.027590704 0.038819698 0.079685319 0.106820925 0.120022758 0.001515282 0.016505793 0.02394277 0.03260442 0.035848973 0.051769975 0.070351415 0.087866067 0.000775163 0.007423975 0.018564336 0.02793626 0.038535534 0.066646136 0.080750505 0.094135124 0.000962637 0.013803218 0.021118597 0.028621412 0.033185146 0.055505577 0.080503187 0.095926878 0.000739132 0.010756004 0.020258788 0.029395541 0.043828324 0.079634763 0.093887046 0.107377616 0.001022109 0.012440449 0.022988625 0.029547433 0.040492324 0.056913395 0.067555363 0.080855957 0.000736435 0.007481556 0.011832101 0.018369203 0.021881196 0.032464622 0.041634057 0.048958597 0.000733583 0.011118267 0.019795222 0.028619113 0.033237191 0.053884332 0.068828904 0.082940086 Phe 0.018676937 0.069984576 0.071160358 0.077161441 0.094726 0.174441367 0.242550604 0.276202385 0.016996125 0.052630975 0.063796081 0.076726044 0.083014983 0.130757145 0.155077127 0.190123458 0.012600041 0.083524811 0.13805496 0.190252397 0.255197836 0.471665867 0.588259941 0.654563328 0.024024755 0.093211211 0.1300863 0.178817022 0.196270882 0.254125122 0.32983381 0.411567476 0.012447641 0.05274408 0.134477886 0.192083638 0.253150264 0.403737309 0.503680307 0.562164874 0.017830912 0.089519415 0.129180673 0.170226611 0.197148574 0.306272544 0.413792425 0.491401628 0.012751703 0.072077205 0.138392711 0.181536803 0.254640034 0.418173993 0.47201865 0.513063669 0.020687484 0.072758807 0.126694768 0.159320269 0.211213975 0.277786524 0.305906096 0.354647638 0.01505301 0.047993112 0.066841799 0.096896542 0.112584475 0.165404581 0.211536599 0.248797498 0.014152323 0.06851487 0.112858514 0.158144929 0.180559966 0.278817207 0.32911195 0.385600039 Pro 0.004393745 0.016486083 0.017688221 0.019192602 0.024888665 0.045040487 0.058840761 0.064359676 0.004027601 0.010593576 0.01399154 0.018500792 0.020968367 0.035281255 0.042555444 0.051403313 0.003715439 0.028367057 0.041340193 0.049756513 0.059776494 0.073541721 0.077478967 0.077348374 0.004807637 0.025000763 0.032457297 0.042508617 0.04447861 0.046402072 0.055037544 0.067578979 0.003620659 0.015318418 0.032784599 0.042512902 0.050928036 0.062036805 0.064475103 0.065606991 0.004026511 0.022623035 0.029835777 0.037418582 0.040220262 0.04515081 0.054929726 0.06564076 0.00337733 0.020321198 0.031907636 0.037641224 0.049709649 0.06439349 0.065487099 0.07310877 0.004154352 0.017795898 0.028394385 0.033825626 0.043106308 0.049369025 0.055014591 0.065055638 0.003483646 0.01394971 0.019744439 0.027477514 0.031110122 0.04103957 0.047895497 0.053455415 0.003497113 0.016237885 0.025867176 0.033457013 0.036153871 0.041818959 0.046017151 0.055542427 Ser 0.004250829 0.013032121 0.015665851 0.018455032 0.027085241 0.075049667 0.111417539 0.120942345 0.003961744 0.009373855 0.012313861 0.017969395 0.022599977 0.051809264 0.074745945 0.092534929 0.003579576 0.057013711 0.092941036 0.123326765 0.152398769 0.175187139 0.14446316 0.133212012 0.004751004 0.049146263 0.081077 0.10719744 0.120597118 0.131801866 0.137050089 0.148123934 0.003176508 0.048239584 0.10844449 0.149297879 0.186160314 0.241921678 0.243116911 0.247982081 0.004766446 0.06530245 0.097542328 0.123587205 0.14181659 0.167857627 0.168024243 0.175357757 0.003170266 0.057492027 0.114379496 0.147764339 0.190158662 0.257931937 0.251317438 0.25356855 0.00481438 0.043904641 0.078665063 0.098771309 0.131672691 0.155851959 0.158408639 0.166286163 0.00352505 0.01373706 0.029578186 0.050800562 0.063308028 0.098057301 0.117780101 0.127953316 0.003307228 0.046560866 0.077552655 0.114913418 0.128033991 0.159755134 0.151806685 0.156522642 Thr 0.008789589 0.044476606 0.046820698 0.048788023 0.060699831 0.111158225 0.150403121 0.165596571 0.007831584 0.031366344 0.038663757 0.047589703 0.053484842 0.082957568 0.100617641 0.117968274 0.006622232 0.056451943 0.091339736 0.120699061 0.155640129 0.242699548 0.2866213 0.309649808 0.009160223 0.05594968 0.081499589 0.104176661 0.113962607 0.137677391 0.166650368 0.206471647 0.005730715 0.037865374 0.082367357 0.116208381 0.149227895 0.226576305 0.261766476 0.294736027 0.00705083 0.055805082 0.077075216 0.094496793 0.109871781 0.146191134 0.181624723 0.215410886 0.00526431 0.039139612 0.071615192 0.092712554 0.124351562 0.213213464 0.248592761 0.278329902 0.006623249 0.037456586 0.063890908 0.079751725 0.104468322 0.133662147 0.151036734 0.175495222 0.005415661 0.022988895 0.033821491 0.048944415 0.057558232 0.083645377 0.10592742 0.12015426 0.005224795 0.035815171 0.057535953 0.080654725 0.089617723 0.129074312 0.147789326 0.174378089 Trp 0.002466314 0.013472322 0.013875853 0.015016599 0.01906549 0.034055397 0.044651334 0.050830036 0.002387409 0.009466071 0.012001206 0.015486805 0.017067461 0.027435996 0.03232535 0.038755499 0.002123507 0.017698866 0.029104872 0.039109134 0.05287973 0.102029743 0.125094895 0.128711971 0.003039018 0.017893286 0.025797774 0.036311292 0.040223588 0.056654755 0.07204376 0.085162945 0.002068233 0.010696634 0.026239391 0.037133834 0.05006557 0.094985764 0.115578218 0.126297843 0.00240116 0.017548252 0.027061346 0.035348597 0.041812556 0.074077121 0.090100829 0.094946627 0.001920301 0.014348114 0.025167544 0.03259032 0.049271077 0.096544224 0.111923478 0.124325711 0.002340228 0.014120972 0.024017937 0.030203114 0.040237182 0.058400654 0.062164133 0.068212036 0.002131254 0.009998801 0.014118426 0.020682847 0.023705017 0.033609524 0.040496486 0.047185664 0.001833301 0.013241121 0.023049336 0.03217574 0.036192499 0.06356519 0.069380704 0.07444946 Tyr 0.006164033 0.026572413 0.027894745 0.030457281 0.039426845 0.072616327 0.098706947 0.110420335 0.005880814 0.019220544 0.0244783 0.031527163 0.035114109 0.057341671 0.069232388 0.083980595 0.005478172 0.032708007 0.054384984 0.073572613 0.097383704 0.171247489 0.223377538 0.249324959 0.007159327 0.034356092 0.051093503 0.070245863 0.077558681 0.101080004 0.129370558 0.158494004 0.005475998 0.021332042 0.050825877 0.072695393 0.096194232 0.160627703 0.206023896 0.240970775 0.00597291 0.033412964 0.04852174 0.065350819 0.075991444 0.116527667 0.153813709 0.178229923 0.005110966 0.026728853 0.051138413 0.067791268 0.096186426 0.169658461 0.200432417 0.227975501 0.006090768 0.027730063 0.047056318 0.059941433 0.08100857 0.10519182 0.11917761 0.13565995 0.005585224 0.018079434 0.025732141 0.037395343 0.043506568 0.063825641 0.080155396 0.090856569 0.005243082 0.025288299 0.041788116 0.059079207 0.068289175 0.105952051 0.123438582 0.143938259 (Iso)Leu 0.067414177 0.271218265 0.275587504 0.294657258 0.356186463 0.595781962 0.762300299 0.836585719 0.06033075 0.198315993 0.237164628 0.287308014 0.307120462 0.466019222 0.538961282 0.639350313 0.045936942 0.27640235 0.433779026 0.580523673 0.759354427 1.227999185 1.455680161 1.555131869 0.075767702 0.299664635 0.403379168 0.539143018 0.577664426 0.672779404 0.845698751 1.04026634 0.045595477 0.181129835 0.419827172 0.589078979 0.765076075 1.137978701 1.3080364 1.340404457 0.058295832 0.288468556 0.403903985 0.517423834 0.57868913 0.793065112 1.021057887 1.189046895 0.045966255 0.251689839 0.444958473 0.569828552 0.796018646 1.240704422 1.40283852 1.497341354 0.067584356 0.251809384 0.409810135 0.496953528 0.640619449 0.782039999 0.873842686 1.022025162 0.050723058 0.174254903 0.234556319 0.330571034 0.371177271 0.507218779 0.612481577 0.698709735 0.048003759 0.222702965 0.35628405 0.481799131 0.536620356 0.743769225 0.857799677 1.003889146 Val 0.025692369 0.076312764 0.074618806 0.078711252 0.089813405 0.134649283 0.168506824 0.18137577 0.023032618 0.057700162 0.06533353 0.075452829 0.077942334 0.108368854 0.120255012 0.139880164 0.012855598 0.062695178 0.093753767 0.12113665 0.157206485 0.262705822 0.321618365 0.352780529 0.02245732 0.065515017 0.082981039 0.109752676 0.117713967 0.147029187 0.183357256 0.222340514 0.011688938 0.038637691 0.082416066 0.112502429 0.142599297 0.220300515 0.2633808 0.286047268 0.016865692 0.058892494 0.077476134 0.098938939 0.111411212 0.161419581 0.21001126 0.245230291 0.012681157 0.055634759 0.089861679 0.108569341 0.151304608 0.232000718 0.261344639 0.285047551 0.020629907 0.055435658 0.082626995 0.096554411 0.123510519 0.154498918 0.170630678 0.194580094 0.012705843 0.035475188 0.04634943 0.062103732 0.068903743 0.09334971 0.114828579 0.130770435 0.01176287 0.042302998 0.065501359 0.08764964 0.097753754 0.143129619 0.165609344 0.191579654 Ala 0.010036564 0.042515512 0.04691593 0.050973081 0.06714905 0.129366772 0.173073127 0.211997255 0.010491917 0.030971098 0.041936373 0.054868992 0.061520453 0.105711146 0.131938798 0.173662029 0.009208693 0.037888852 0.034761104 0.023262389 0.021140281 0.0572668 0.073511371 0.079146049 0.013589337 0.06191181 0.05164086 0.049909407 0.034884613 0.042885209 0.07967651 0.122165305 0.0102047 0.031868647 0.03101492 0.029185609 0.030034202 0.083422853 0.12311896 0.136860435 0.014347957 0.05666285 0.048905422 0.043113835 0.03320145 0.055951505 0.090933817 0.134840464 0.010129581 0.038802539 0.025011596 0.019902419 0.020279415 0.064498254 0.125238092 0.177875195 0.014300752 0.055867991 0.071289168 0.068859895 0.074088093 0.088917131 0.132262831 0.183401096 0.010926967 0.036202909 0.054074029 0.071781864 0.077498054 0.108459751 0.154264671 0.200194353 0.010861191 0.050867967 0.061240481 0.061047208 0.053738853 0.059902754 0.107140732 0.158680759 Arg 0.002472393 0.003655326 0.003709353 0.003527192 0.004016422 0.005708968 0.005776538 0.005119655 0.002432506 0.002900911 0.003170132 0.003387849 0.003347762 0.003904845 0.003481496 0.003169584 0.002536537 0.005524146 0.007450781 0.007806013 0.008327671 0.007895051 0.008940939 0.008523675 0.002737998 0.004699164 0.006316131 0.006845787 0.005285173 0.003379343 0.003674765 0.004129058 0.002691975 0.004257208 0.007282363 0.009842898 0.010625067 0.01255643 0.011617519 0.009817835 0.002669642 0.005567569 0.007268987 0.008051236 0.006461382 0.004766424 0.005586491 0.006388483 0.002569428 0.004466765 0.006891853 0.007642767 0.009205695 0.010984967 0.010772174 0.009963101 0.002740249 0.003630703 0.005432959 0.005472214 0.005305894 0.004302366 0.004564478 0.005036281 0.002475706 0.002531472 0.002695633 0.003555904 0.003301096 0.002620405 0.002025019 0.002192935 0.002348137 0.003912585 0.005957462 0.006159122 0.005403843 0.003656015 0.003275943 0.003650631 Asn 0.001076807 0.0025563 0.003300715 0.003569559 0.005128706 0.012172331 0.017800715 0.018015466 0.001086061 0.001707663 0.002319839 0.003288583 0.003802486 0.006678984 0.00850592 0.009767577 0.001078867 0.013175172 0.02194088 0.030978651 0.041318158 0.059974181 0.057093545 0.054436675 0.001084935 0.010927318 0.017436727 0.022485723 0.023959025 0.024538626 0.026853414 0.029184035 0.001138032 0.008309572 0.020267861 0.030417308 0.042400996 0.05796229 0.050483656 0.047926091 0.001115358 0.011767753 0.017880656 0.02263469 0.025712513 0.027213265 0.030457941 0.033962577 0.001085133 0.007620951 0.017996786 0.020648182 0.03029004 0.05862184 0.053210112 0.048849483 0.00111206 0.006752502 0.011761462 0.015711923 0.020549551 0.022904413 0.022770263 0.019395139 0.001081174 0.002679048 0.004550582 0.007254779 0.00867682 0.012050292 0.01358154 0.013852879 0.001076988 0.008149818 0.013291265 0.019033202 0.021144447 0.023800334 0.020836287 0.020858207 Glu 0.079397632 0.150602976 0.160862168 0.172422901 0.218284257 0.295815441 0.286027024 0.268356013 0.080079956 0.116636617 0.146061268 0.193026749 0.218653606 0.341381256 0.389157378 0.434494207 0.079549543 0.251497936 0.280727708 0.290106492 0.270655677 0.100927992 0.094245169 0.096176062 0.07999695 0.192430164 0.217836696 0.241505048 0.254834517 0.137345457 0.117617096 0.126095749 0.08391202 0.223402108 0.277021198 0.337935385 0.34842248 0.113967125 0.094707987 0.107774308 0.082240179 0.200976861 0.259503126 0.291454903 0.297143933 0.132273899 0.113681558 0.128368108 0.080011554 0.185032262 0.248518088 0.357233794 0.353552462 0.137677262 0.112723333 0.118501992 0.081996987 0.227102043 0.257881788 0.246670062 0.292222075 0.251793047 0.226002872 0.231186461 0.079719643 0.120049456 0.212665434 0.301214096 0.338772903 0.433452768 0.450245784 0.436164825 0.079410964 0.181141802 0.234282299 0.296672993 0.327643592 0.312593398 0.217940904 0.207801361 Ornitine 0.003414583 0.004910796 0.005585691 0.006230573 0.007031796 0.011639354 0.015324445 0.016720111 0.003443927 0.003665939 0.004727784 0.005961917 0.006102831 0.009897748 0.011680904 0.013558435 0.003421116 0.006432334 0.010650231 0.014222276 0.017238937 0.02914338 0.036539764 0.038313659 0.003440357 0.006810615 0.009359106 0.01234595 0.012577068 0.015720489 0.018102662 0.020853167 0.003608729 0.004872291 0.008578706 0.012190265 0.014850798 0.026001013 0.033091651 0.036183747 0.00353683 0.007408536 0.008551489 0.010811095 0.011837017 0.015349548 0.017423901 0.018819191 0.003440985 0.005570877 0.009483939 0.012474491 0.016903956 0.027270406 0.033856513 0.037711554 0.003526371 0.006011656 0.008900923 0.010940167 0.013083008 0.016202772 0.017247597 0.019087902 0.003428431 0.00443172 0.006405046 0.008944682 0.009294626 0.013374478 0.016011531 0.017163007 0.003415156 0.005370297 0.008301067 0.011041332 0.011424059 0.016105358 0.016064505 0.017321767 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Glucose Lactate Gly Gln Lys Met Phe Pro Ser Thr Trp Tyr (Iso)Leu Val Ala Arg Asn Glu Ornitine METABOLITES_END #END