#METABOLOMICS WORKBENCH engelmab_20231008_001358 DATATRACK_ID:4381 STUDY_ID:ST002919 ANALYSIS_ID:AN004789 PROJECT_ID:PR001814
VERSION             	1
CREATED_ON             	October 10, 2023, 2:15 am
#PROJECT
PR:PROJECT_TITLE                 	MINCH causes metabolic rewiring towards lipid accumulation and adipogenesis
PR:PROJECT_SUMMARY               	Humans are ubiquitously exposed to plastic additives, including plasticizers.
PR:PROJECT_SUMMARY               	There is growing evidence that exposure to certain plasticizers is associated
PR:PROJECT_SUMMARY               	with the development of obesity due to their metabolism-disrupting properties.
PR:PROJECT_SUMMARY               	Following the restriction of the use of the phthalate plasticizer
PR:PROJECT_SUMMARY               	di-(2-ethylhexyl) phthalate (DEHP) due to its adverse health effects, it has
PR:PROJECT_SUMMARY               	been replaced by new substitutes such as the plasticizer
PR:PROJECT_SUMMARY               	diisononylcyclohexane-1,2-dicarboxylate (DINCH). Despite recent studies
PR:PROJECT_SUMMARY               	suggesting that the primary metabolite monoisononylcyclohexane-1,2-dicarboxylic
PR:PROJECT_SUMMARY               	acid ester (MINCH) promotes human adipocyte differentiation, the adipogenic
PR:PROJECT_SUMMARY               	properties of MINCH remain controversial. Because the metabolome largely
PR:PROJECT_SUMMARY               	reflects the molecular phenotype and is sensitive to perturbation by external
PR:PROJECT_SUMMARY               	factors, we used targeted metabolomics to investigate the effects of DINCH and
PR:PROJECT_SUMMARY               	MINCH on key metabolic pathways of adipocytes. Analysis of central carbon
PR:PROJECT_SUMMARY               	metabolism is particularly relevant because it provides cellular energy through
PR:PROJECT_SUMMARY               	the degradation of organic compounds and metabolic precursors for anabolic
PR:PROJECT_SUMMARY               	functions that are critical for adipocyte function, such as de novo lipogenesis.
PR:PROJECT_SUMMARY               	The project consists of three main studies: analysis of the effects of DINCH and
PR:PROJECT_SUMMARY               	MINCH on central carbon metabolism of human SGSB cells, analysis of the insulin
PR:PROJECT_SUMMARY               	response of DINCH- and MINCH-treated SGSB cells, and analysis of the effects of
PR:PROJECT_SUMMARY               	DINCH and MINCH on central carbon metabolism of human SGSB cells in the presence
PR:PROJECT_SUMMARY               	of the PPARG inhibitor GW9662.
PR:INSTITUTE                     	Helmholtz Centre for Environmental Research
PR:LAST_NAME                     	Engelmann
PR:FIRST_NAME                    	Beatrice
PR:ADDRESS                       	Permoserstr. 15
PR:EMAIL                         	beatrice.engelmann@ufz.de
PR:PHONE                         	00493412351099
#STUDY
ST:STUDY_TITLE                   	Short-term metabolic insulin response of DINCH- and MINCH-treated cells
ST:STUDY_SUMMARY                 	In the second part of the project, we examined the short-term metabolic insulin
ST:STUDY_SUMMARY                 	response of DINAH- and MINCH-treated cells and compared them with
ST:STUDY_SUMMARY                 	rosiglitazone-differentiated cells. For this purpose, the human SGBS
ST:STUDY_SUMMARY                 	preadipocyte cell line was exposed to differentiation media conditioned with
ST:STUDY_SUMMARY                 	DINCH (10 nM or 10 µM), MINCH (10 nM or 10 µM), or rosiglitazone, and the
ST:STUDY_SUMMARY                 	insulin response was measured by analyzing the changes in glycolysis and PPP
ST:STUDY_SUMMARY                 	between insulin-stimulated and non-insulin-stimulated cells. In conclusion, the
ST:STUDY_SUMMARY                 	insulin response of glycolysis and PPP of cells treated with 10 µM MINCH, but
ST:STUDY_SUMMARY                 	not with 10 nM MINCH or DINCH, was similar to cells differentiated with
ST:STUDY_SUMMARY                 	rosiglitazone.
ST:INSTITUTE                     	Helmholtz Centre for Environmental Research
ST:LAST_NAME                     	Engelmann
ST:FIRST_NAME                    	Beatrice
ST:ADDRESS                       	Permoserstr. 15
ST:EMAIL                         	beatrice.engelmann@ufz.de
ST:PHONE                         	00493412351099
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
SU:GENDER                        	Male
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_-_1	Treatment:DINCH 10µM minus Insulin | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_-_2	Treatment:DINCH 10µM minus Insulin | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_-_3	Treatment:DINCH 10µM minus Insulin | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_-_4	Treatment:DINCH 10µM minus Insulin | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_-_5	Treatment:DINCH 10µM minus Insulin | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_+_1	Treatment:DINCH 10µM plus Insulin | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_+_2	Treatment:DINCH 10µM plus Insulin | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_+_3	Treatment:DINCH 10µM plus Insulin | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_+_4	Treatment:DINCH 10µM plus Insulin | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_+_5	Treatment:DINCH 10µM plus Insulin | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_-_1	Treatment:DINCH 10nM minus Insulin | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_-_2	Treatment:DINCH 10nM minus Insulin | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_-_3	Treatment:DINCH 10nM minus Insulin | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_-_4	Treatment:DINCH 10nM minus Insulin | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_-_5	Treatment:DINCH 10nM minus Insulin | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_+_1	Treatment:DINCH 10nM plus Insulin | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_+_2	Treatment:DINCH 10nM plus Insulin | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_+_3	Treatment:DINCH 10nM plus Insulin | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_+_4	Treatment:DINCH 10nM plus Insulin | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_+_5	Treatment:DINCH 10nM plus Insulin | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_-_1	Treatment:DINCH 10µM minus Insulin | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_-_2	Treatment:DINCH 10µM minus Insulin | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_-_3	Treatment:DINCH 10µM minus Insulin | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_-_4	Treatment:DINCH 10µM minus Insulin | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_-_5	Treatment:DINCH 10µM minus Insulin | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_+_1	Treatment:DINCH 10µM plus Insulin | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_+_2	Treatment:DINCH 10µM plus Insulin | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_+_3	Treatment:DINCH 10µM plus Insulin | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_+_4	Treatment:DINCH 10µM plus Insulin | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_+_5	Treatment:DINCH 10µM plus Insulin | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_-_1	Treatment:DINCH 10nM minus Insulin | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_-_2	Treatment:DINCH 10nM minus Insulin | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_-_3	Treatment:DINCH 10nM minus Insulin | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_-_4	Treatment:DINCH 10nM minus Insulin | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_-_5	Treatment:DINCH 10nM minus Insulin | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_+_1	Treatment:DINCH 10nM plus Insulin | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_+_2	Treatment:DINCH 10nM plus Insulin | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_+_3	Treatment:DINCH 10nM plus Insulin | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_+_4	Treatment:DINCH 10nM plus Insulin | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_+_5	Treatment:DINCH 10nM plus Insulin | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_-_1	Treatment:Rosiglitazone minus insulin | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_-_2	Treatment:Rosiglitazone minus insulin | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_-_3	Treatment:Rosiglitazone minus insulin | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_-_4	Treatment:Rosiglitazone minus insulin | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_-_5	Treatment:Rosiglitazone minus insulin | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_+_1	Treatment:Rosiglitazone plus insulin | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_+_2	Treatment:Rosiglitazone plus insulin | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_+_3	Treatment:Rosiglitazone plus insulin | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_+_4	Treatment:Rosiglitazone plus insulin | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_+_5	Treatment:Rosiglitazone plus insulin | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_SN_-_1	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_SN_-_2	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_SN_-_3	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_SN_-_4	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_SN_-_5	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_SN_+_1	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_SN_+_2	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_SN_+_3	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_SN_+_4	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_High_SN_+_5	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_SN_-_1	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_SN_-_2	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_SN_-_3	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_SN_-_4	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_SN_-_5	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_SN_+_1	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_SN_+_2	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_SN_+_3	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_SN_+_4	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	DINCH_Low_SN_+_5	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_SN_-_1	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_SN_-_2	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_SN_-_3	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_SN_-_4	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_SN_-_5	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_SN_+_1	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_SN_+_2	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_SN_+_3	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_SN_+_4	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_High_SN_+_5	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_SN_-_1	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_SN_-_2	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_SN_-_3	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_SN_-_4	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_SN_-_5	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_SN_+_1	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_SN_+_2	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_SN_+_3	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_SN_+_4	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	MINCH_Low_SN_+_5	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_SN_-_1	Treatment:Rosiglitazone minus insulin supernatant | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_SN_-_2	Treatment:Rosiglitazone minus insulin supernatant | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_SN_-_3	Treatment:Rosiglitazone minus insulin supernatant | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_SN_-_4	Treatment:Rosiglitazone minus insulin supernatant | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_SN_-_5	Treatment:Rosiglitazone minus insulin supernatant | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_SN_+_1	Treatment:Rosiglitazone plus insulin supernatant | Replicate:1	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_SN_+_2	Treatment:Rosiglitazone plus insulin supernatant | Replicate:2	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_SN_+_3	Treatment:Rosiglitazone plus insulin supernatant | Replicate:3	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_SN_+_4	Treatment:Rosiglitazone plus insulin supernatant | Replicate:4	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
SUBJECT_SAMPLE_FACTORS           	-	Rosi_SN_+_5	Treatment:Rosiglitazone plus insulin supernatant | Replicate:5	Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff
#COLLECTION
CO:COLLECTION_SUMMARY            	The SGBS cells were obtained from Prof. Martin Wabitsch laboratory at the
CO:COLLECTION_SUMMARY            	University Clinic Ulm. SGBS preadipocytes were differentiated according to the
CO:COLLECTION_SUMMARY            	standard protocol described previously (Wabitsch et al., 2001).
CO:SAMPLE_TYPE                   	Adipose tissue
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	SGSB preadipocytes were maintained at 37°C and 5% CO2 in 95% humidity. To
TR:TREATMENT_SUMMARY             	examine the insulin response of SGBS treated with DINCH and MINCH, SGBS
TR:TREATMENT_SUMMARY             	preadipocytes were treated for 18 days with differentiation media without the
TR:TREATMENT_SUMMARY             	PPARG agonist rosiglitazone supplemented with DINCH or MINCH (10 nM and 10 µM).
TR:TREATMENT_SUMMARY             	To obtain an adipogenesis reference, SGBS cells were differentiated with
TR:TREATMENT_SUMMARY             	rosiglitazone for 18 days according to the standard differentiation protocol.
TR:TREATMENT_SUMMARY             	After replacing the medium on day 17 with differentiation medium without insulin
TR:TREATMENT_SUMMARY             	and overnight incubation (insulin starvation), cells were starved for 1 hour
TR:TREATMENT_SUMMARY             	with differentiation medium without glucose and insulin (glucose + insulin
TR:TREATMENT_SUMMARY             	starvation). Subsequently, cells were incubated with either differentiation
TR:TREATMENT_SUMMARY             	medium with insulin (insulin-stimulated cells correspond to plus-insulin
TR:TREATMENT_SUMMARY             	samples) or without insulin (unstimulated cells correspond to minus-insulin
TR:TREATMENT_SUMMARY             	samples). During differentiation, a final concentration of 0.01% (v/v) MeOH and
TR:TREATMENT_SUMMARY             	0.02% (v/v) DMSO was added to all differentiation media. Continuous exposure was
TR:TREATMENT_SUMMARY             	mimicked by replacing the cell culture medium every other day. Each treatment
TR:TREATMENT_SUMMARY             	was performed in five biological replicates (n=5).
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Extraction of intracellular and extracellular metabolites was performed by a
SP:SAMPLEPREP_SUMMARY            	1:1:1 methanol:water:chloroform extraction protocol. For the extraction of
SP:SAMPLEPREP_SUMMARY            	intracellular metabolites, the culture medium was removed and the cells were
SP:SAMPLEPREP_SUMMARY            	rinsed twice with 1 ml of 0.9% ice-cold NaCl. The rinsing solution was removed,
SP:SAMPLEPREP_SUMMARY            	and the metabolism of the cells was stopped by adding 400 µL of MeOH (-20 °C)
SP:SAMPLEPREP_SUMMARY            	followed by 400 µL of ice-cold H2O containing 10 µM d6-glutarate. Cells were
SP:SAMPLEPREP_SUMMARY            	collected using a cell lifter and 400 µL of chloroform was added. After shaking
SP:SAMPLEPREP_SUMMARY            	at 1,400 rpm and 4 °C for 20 min, the extraction mixture was centrifuged at
SP:SAMPLEPREP_SUMMARY            	18,000 g and 4 °C for 5 min. Subsequently, 300 µL volume of the polar upper
SP:SAMPLEPREP_SUMMARY            	phase was collected and evaporated to complete dryness. For the extraction of
SP:SAMPLEPREP_SUMMARY            	extracellular metabolites, 300 µL of the supernatant was extracted by adding
SP:SAMPLEPREP_SUMMARY            	400 µL MeOH (-20 °C) containing 100 nM MEHP, 100 µL ice-cold H2O containing
SP:SAMPLEPREP_SUMMARY            	40 µM d6-glutarate, and 400 µL chloroform (-20 °C). Subsequent sample
SP:SAMPLEPREP_SUMMARY            	preparation was identical to the extraction of intracellular metabolites. Note:
SP:SAMPLEPREP_SUMMARY            	After measurement of the samples by LC-MS, the raw AUC values uploaded here were
SP:SAMPLEPREP_SUMMARY            	normalized to the internal standard (d6-glutarate, if applicable) and DNA
SP:SAMPLEPREP_SUMMARY            	content per well (measured by DAPI fluorescence). After normalization, log2 fold
SP:SAMPLEPREP_SUMMARY            	changes were calculated by dividing the normalized peak area from each replicate
SP:SAMPLEPREP_SUMMARY            	of each treatment by the normalized peak area from each control. Insulin data
SP:SAMPLEPREP_SUMMARY            	were not normalized to DAPI because fold changes were calculated by dividing the
SP:SAMPLEPREP_SUMMARY            	intensities of the insulin-stimulated cells by the noninsulin-stimulated cells
SP:SAMPLEPREP_SUMMARY            	from each treatment.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity II
CH:COLUMN_NAME                   	Waters Xselect XP HSS T3 (150 x 2.1mm, 2.5µm)
CH:SOLVENT_A                     	10mM TBA, 10mM acetic acid, 5% MeOH, 2% IPA in water
CH:SOLVENT_B                     	100% IPA
CH:FLOW_GRADIENT                 	0-5 min 0% B, 5-9 min 0%- 2% B, 9-9.5 min 2-6% B, 9.5-11.5 min 6% B, 11.5-12 min
CH:FLOW_GRADIENT                 	6-11% B, 12-13.5 min 11% B, 13.5-15.5 min 11-28% B, 15.5-16.5 min 28-53% B,
CH:FLOW_GRADIENT                 	16.5-22.5 53% B, 22.5-23 min 53-0% B, 23-33 min 0% B
CH:FLOW_RATE                     	0-15.5 min 0.4 mL/min, 15.5-16.5 min 0.4-0.15 mL/min, 16.5-23 min 0.15 mL/min,
CH:FLOW_RATE                     	23-27 min 0.15-0.4 mL/min, 27-33 min 0.4 mL/min
CH:COLUMN_TEMPERATURE            	40
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	ABI Sciex 6500+
MS:INSTRUMENT_TYPE               	QTRAP
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	For identification and quantitation, a scheduled MRM method was used, with
MS:MS_COMMENTS                   	specific transitions for every metabolite. Data acquisition and peak integration
MS:MS_COMMENTS                   	were performed in Analyst® software (Version 1.7.1).
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	Peak AUC
MS_METABOLITE_DATA_START
Samples	DINCH_High_-_1	DINCH_High_-_2	DINCH_High_-_3	DINCH_High_-_4	DINCH_High_-_5	DINCH_High_+_1	DINCH_High_+_2	DINCH_High_+_3	DINCH_High_+_4	DINCH_High_+_5	DINCH_Low_-_1	DINCH_Low_-_2	DINCH_Low_-_3	DINCH_Low_-_4	DINCH_Low_-_5	DINCH_Low_+_1	DINCH_Low_+_2	DINCH_Low_+_3	DINCH_Low_+_4	DINCH_Low_+_5	MINCH_High_-_1	MINCH_High_-_2	MINCH_High_-_3	MINCH_High_-_4	MINCH_High_-_5	MINCH_High_+_1	MINCH_High_+_2	MINCH_High_+_3	MINCH_High_+_4	MINCH_High_+_5	MINCH_Low_-_1	MINCH_Low_-_2	MINCH_Low_-_3	MINCH_Low_-_4	MINCH_Low_-_5	MINCH_Low_+_1	MINCH_Low_+_2	MINCH_Low_+_3	MINCH_Low_+_4	MINCH_Low_+_5	Rosi_-_1	Rosi_-_2	Rosi_-_3	Rosi_-_4	Rosi_-_5	Rosi_+_1	Rosi_+_2	Rosi_+_3	Rosi_+_4	Rosi_+_5	DINCH_High_SN_-_1	DINCH_High_SN_-_2	DINCH_High_SN_-_3	DINCH_High_SN_-_4	DINCH_High_SN_-_5	DINCH_High_SN_+_1	DINCH_High_SN_+_2	DINCH_High_SN_+_3	DINCH_High_SN_+_4	DINCH_High_SN_+_5	DINCH_Low_SN_-_1	DINCH_Low_SN_-_2	DINCH_Low_SN_-_3	DINCH_Low_SN_-_4	DINCH_Low_SN_-_5	DINCH_Low_SN_+_1	DINCH_Low_SN_+_2	DINCH_Low_SN_+_3	DINCH_Low_SN_+_4	DINCH_Low_SN_+_5	MINCH_High_SN_-_1	MINCH_High_SN_-_2	MINCH_High_SN_-_3	MINCH_High_SN_-_4	MINCH_High_SN_-_5	MINCH_High_SN_+_1	MINCH_High_SN_+_2	MINCH_High_SN_+_3	MINCH_High_SN_+_4	MINCH_High_SN_+_5	MINCH_Low_SN_-_1	MINCH_Low_SN_-_2	MINCH_Low_SN_-_3	MINCH_Low_SN_-_4	MINCH_Low_SN_-_5	MINCH_Low_SN_+_1	MINCH_Low_SN_+_2	MINCH_Low_SN_+_3	MINCH_Low_SN_+_4	MINCH_Low_SN_+_5	Rosi_SN_-_1	Rosi_SN_-_2	Rosi_SN_-_3	Rosi_SN_-_4	Rosi_SN_-_5	Rosi_SN_+_1	Rosi_SN_+_2	Rosi_SN_+_3	Rosi_SN_+_4	Rosi_SN_+_5
Factors	Treatment:DINCH 10µM minus Insulin | Replicate:1	Treatment:DINCH 10µM minus Insulin | Replicate:2	Treatment:DINCH 10µM minus Insulin | Replicate:3	Treatment:DINCH 10µM minus Insulin | Replicate:4	Treatment:DINCH 10µM minus Insulin | Replicate:5	Treatment:DINCH 10µM plus Insulin | Replicate:1	Treatment:DINCH 10µM plus Insulin | Replicate:2	Treatment:DINCH 10µM plus Insulin | Replicate:3	Treatment:DINCH 10µM plus Insulin | Replicate:4	Treatment:DINCH 10µM plus Insulin | Replicate:5	Treatment:DINCH 10nM minus Insulin | Replicate:1	Treatment:DINCH 10nM minus Insulin | Replicate:2	Treatment:DINCH 10nM minus Insulin | Replicate:3	Treatment:DINCH 10nM minus Insulin | Replicate:4	Treatment:DINCH 10nM minus Insulin | Replicate:5	Treatment:DINCH 10nM plus Insulin | Replicate:1	Treatment:DINCH 10nM plus Insulin | Replicate:2	Treatment:DINCH 10nM plus Insulin | Replicate:3	Treatment:DINCH 10nM plus Insulin | Replicate:4	Treatment:DINCH 10nM plus Insulin | Replicate:5	Treatment:DINCH 10µM minus Insulin | Replicate:1	Treatment:DINCH 10µM minus Insulin | Replicate:2	Treatment:DINCH 10µM minus Insulin | Replicate:3	Treatment:DINCH 10µM minus Insulin | Replicate:4	Treatment:DINCH 10µM minus Insulin | Replicate:5	Treatment:DINCH 10µM plus Insulin | Replicate:1	Treatment:DINCH 10µM plus Insulin | Replicate:2	Treatment:DINCH 10µM plus Insulin | Replicate:3	Treatment:DINCH 10µM plus Insulin | Replicate:4	Treatment:DINCH 10µM plus Insulin | Replicate:5	Treatment:DINCH 10nM minus Insulin | Replicate:1	Treatment:DINCH 10nM minus Insulin | Replicate:2	Treatment:DINCH 10nM minus Insulin | Replicate:3	Treatment:DINCH 10nM minus Insulin | Replicate:4	Treatment:DINCH 10nM minus Insulin | Replicate:5	Treatment:DINCH 10nM plus Insulin | Replicate:1	Treatment:DINCH 10nM plus Insulin | Replicate:2	Treatment:DINCH 10nM plus Insulin | Replicate:3	Treatment:DINCH 10nM plus Insulin | Replicate:4	Treatment:DINCH 10nM plus Insulin | Replicate:5	Treatment:Rosiglitazone minus insulin | Replicate:1	Treatment:Rosiglitazone minus insulin | Replicate:2	Treatment:Rosiglitazone minus insulin | Replicate:3	Treatment:Rosiglitazone minus insulin | Replicate:4	Treatment:Rosiglitazone minus insulin | Replicate:5	Treatment:Rosiglitazone plus insulin | Replicate:1	Treatment:Rosiglitazone plus insulin | Replicate:2	Treatment:Rosiglitazone plus insulin | Replicate:3	Treatment:Rosiglitazone plus insulin | Replicate:4	Treatment:Rosiglitazone plus insulin | Replicate:5	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:1	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:2	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:3	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:4	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:5	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:1	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:2	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:3	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:4	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:5	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:1	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:2	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:3	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:4	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:5	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:1	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:2	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:3	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:4	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:5	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:1	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:2	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:3	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:4	Treatment:DINCH 10µM minus Insulin supernatant | Replicate:5	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:1	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:2	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:3	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:4	Treatment:DINCH 10µM plus Insulin supernatant | Replicate:5	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:1	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:2	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:3	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:4	Treatment:DINCH 10nM minus Insulin supernatant | Replicate:5	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:1	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:2	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:3	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:4	Treatment:DINCH 10nM plus Insulin supernatant | Replicate:5	Treatment:Rosiglitazone minus insulin supernatant | Replicate:1	Treatment:Rosiglitazone minus insulin supernatant | Replicate:2	Treatment:Rosiglitazone minus insulin supernatant | Replicate:3	Treatment:Rosiglitazone minus insulin supernatant | Replicate:4	Treatment:Rosiglitazone minus insulin supernatant | Replicate:5	Treatment:Rosiglitazone plus insulin supernatant | Replicate:1	Treatment:Rosiglitazone plus insulin supernatant | Replicate:2	Treatment:Rosiglitazone plus insulin supernatant | Replicate:3	Treatment:Rosiglitazone plus insulin supernatant | Replicate:4	Treatment:Rosiglitazone plus insulin supernatant | Replicate:5
Glucose 6-phosphate	4.10E+03	2.44E+03	2.35E+03	3.52E+03	3.85E+03	5.45E+03	5.64E+03	6.70E+03	5.64E+03	4.28E+03	2.03E+03	1.57E+03	9.08E+02	1.93E+03		2.01E+03	1.33E+03		1.58E+03	9.11E+02	1.28E+04	1.34E+04	1.14E+04	1.04E+04	8.19E+03	1.13E+05	1.09E+05	8.74E+04	8.77E+04	8.22E+04	1.51E+03	1.85E+03	7.95E+02		2.12E+03	1.93E+03	1.11E+03	2.31E+03		1.80E+03	1.82E+04	1.23E+04	1.24E+04	1.22E+04	1.03E+04	1.06E+05	1.20E+05	1.21E+05	1.20E+05	1.33E+05																																																		
Lactate	1.01E+06	1.22E+06	1.89E+06	6.44E+06	1.64E+07	1.82E+06	1.83E+06	2.14E+07	1.16E+07	2.86E+06	1.47E+06	1.87E+06	2.16E+06	1.30E+06	1.04E+07	1.89E+06	1.95E+06	3.68E+06	1.96E+06	1.78E+06	1.48E+06	1.67E+06	3.19E+06	1.69E+06	1.70E+06	2.01E+06	2.03E+06	2.24E+06	1.67E+06	2.12E+06	2.35E+06	2.19E+06	2.49E+06	1.76E+06	3.24E+06	4.33E+06	1.43E+06	2.67E+06	2.13E+06	1.31E+06	1.27E+06	1.20E+06	1.35E+06	7.49E+05	9.68E+05	1.64E+06	1.17E+06	1.63E+06	1.21E+06	1.50E+06	5.69E+05	4.72E+05	6.35E+05	5.08E+05	5.46E+05	6.05E+05	7.64E+05	5.66E+05	5.98E+05	5.87E+05	7.48E+05	7.55E+05	6.40E+05	7.52E+05	4.77E+05	4.28E+05	4.70E+05	4.88E+05	6.48E+05	4.42E+05	9.21E+05	1.04E+06	1.07E+06	1.01E+06	8.74E+05	1.84E+06	1.77E+06	1.65E+06	1.72E+06	1.67E+06	5.75E+05	6.61E+05	5.74E+05	6.01E+05	5.62E+05	7.83E+05	5.62E+05	5.55E+05	5.71E+05	6.04E+05	9.43E+05	1.21E+06	7.44E+05	8.49E+05	1.35E+06	2.10E+06	2.25E+06	2.21E+06	1.80E+06	2.31E+06
Alanine	6.07E+03	8.61E+03	6.06E+03	6.53E+03	1.50E+04	4.35E+03	5.65E+03	1.84E+04	1.18E+04	5.32E+03	4.37E+03	4.69E+03	5.14E+03	4.97E+03	6.49E+03	9.58E+03	6.88E+03	6.19E+03	4.47E+03	4.69E+03	6.44E+03	6.95E+03	6.30E+03	6.83E+03	9.60E+03	6.40E+03	6.35E+03	6.31E+03	5.89E+03	6.60E+03	5.20E+03	4.57E+03	4.61E+03	4.42E+03	4.90E+03	5.74E+03	6.43E+03	7.65E+03	4.95E+03	4.58E+03	8.41E+03	7.85E+03	9.17E+03	8.79E+03	7.77E+03	8.18E+03	7.68E+03	7.70E+03	9.50E+03	8.63E+03																																																		
Phosphoenolpyruvate	5.44E+04	4.97E+04	5.49E+04	4.52E+04	4.77E+04	4.69E+04	5.02E+04	6.47E+04	5.09E+04	4.82E+04	4.21E+04	4.51E+04	4.69E+04	4.37E+04	4.35E+04	4.90E+04	4.11E+04	4.52E+04	4.57E+04	4.20E+04	2.52E+05	2.12E+05	1.89E+05	2.14E+05	1.73E+05	3.59E+05	3.23E+05	2.60E+05	2.87E+05	2.64E+05	4.76E+04	4.67E+04	4.40E+04	4.39E+04	4.89E+04	4.51E+04	5.27E+04	4.91E+04	4.42E+04	4.36E+04	1.46E+05	1.20E+05	1.05E+05	1.41E+05	1.83E+05	5.54E+05	4.66E+05	4.26E+05	4.32E+05	5.14E+05																																																		
Pyruvate	5.35E+05	8.14E+05	2.99E+05	1.88E+05	2.49E+05	2.02E+05	2.61E+05	4.06E+05	2.02E+05	1.47E+05	1.97E+05	2.14E+05	2.22E+05	3.00E+05	3.55E+05	2.29E+06	3.07E+05	1.27E+05	1.00E+05	1.36E+05	2.71E+05	3.19E+05	1.98E+05	4.51E+05	1.54E+06	2.34E+05	3.22E+05	2.67E+05	3.50E+05	3.01E+05	2.58E+05	1.46E+05	1.70E+05	2.45E+05	1.91E+05	1.47E+05	1.25E+06	8.32E+05	2.02E+05	2.06E+05	6.56E+05	5.19E+05	7.77E+05	1.34E+06	1.49E+06	5.42E+05	4.83E+05	5.31E+05	1.42E+06	1.01E+06																																																		
Fructose 6-phosphate	9.35E+03	4.08E+03	6.51E+03	4.76E+03	7.76E+03	6.85E+03	1.27E+04	1.07E+04	9.23E+03	6.62E+03	3.37E+03	3.43E+03	2.44E+03	3.21E+03	4.23E+03	2.04E+03	3.94E+03	2.42E+03	2.39E+03	2.71E+03	1.31E+04	2.33E+04	1.13E+04	1.77E+04	1.28E+04	2.67E+05	2.58E+05	2.01E+05	2.08E+05	1.89E+05	2.62E+03	3.55E+03	2.64E+03	3.68E+03	2.54E+03	4.22E+03	2.14E+03	3.68E+03	3.90E+03	3.63E+03	2.82E+04	2.31E+04	1.95E+04	1.51E+04	1.39E+04	2.71E+05	2.98E+05	2.73E+05	2.59E+05	3.07E+05																																																		
Ribulose 5-phosphate	1.63E+04	1.67E+04	1.69E+04	1.58E+04	1.33E+04	1.63E+04	1.81E+04	1.80E+04	1.80E+04	1.68E+04	1.48E+04	1.67E+04	1.58E+04	1.49E+04	1.54E+04	1.50E+04	1.30E+04	1.55E+04	1.58E+04	1.41E+04	1.92E+04	1.93E+04	1.51E+04	1.87E+04	1.48E+04	1.47E+05	1.45E+05	1.22E+05	1.24E+05	1.32E+05	8.96E+03	1.16E+04	1.42E+04	1.14E+04	1.20E+04	1.13E+04	1.07E+04	1.20E+04	1.20E+04	1.06E+04	1.68E+04	1.21E+04	8.87E+03	1.10E+04	1.13E+04	1.41E+05	1.21E+05	1.29E+05	1.20E+05	1.50E+05																																																		
Ribose 5-phosphate	5.52E+03	7.14E+03	5.65E+03	8.14E+03	5.76E+03	7.62E+03	9.57E+03	7.59E+03	7.80E+03	7.20E+03	6.47E+03	5.62E+03	7.06E+03	6.68E+03	7.21E+03	5.01E+03	3.79E+03	6.76E+03	6.12E+03	6.03E+03	1.32E+04	8.80E+03	8.50E+03	7.15E+03	7.34E+03	7.03E+04	7.09E+04	5.30E+04	5.17E+04	5.04E+04	5.11E+03	6.18E+03	5.15E+03	3.78E+03	4.71E+03	4.74E+03	3.17E+03	4.96E+03	4.99E+03	4.58E+03	1.05E+04	5.78E+03	8.50E+03	5.00E+03	7.41E+03	5.29E+04	4.20E+04	4.94E+04	3.96E+04	5.19E+04																																																		
Fructose 1,6-bisphosphate	8.10E+03	1.76E+04	1.13E+04	1.60E+04	6.22E+03	3.10E+04	2.63E+04	3.37E+04	3.54E+04	2.07E+04	9.27E+03	8.76E+03	7.79E+03	5.80E+03	1.28E+04	2.21E+04	9.69E+03	1.49E+04	1.21E+04	1.06E+04	4.14E+04	3.72E+04	2.95E+04	3.80E+04	4.09E+04	2.63E+06	3.04E+06	1.90E+06	2.12E+06	2.33E+06	5.60E+03	1.03E+04	6.15E+03	4.49E+03	5.52E+03	7.63E+03	1.45E+04	1.30E+04	1.56E+04	6.86E+03	4.19E+04	1.62E+04	1.57E+04	2.17E+04	2.42E+04	1.55E+06	1.10E+06	1.09E+06	1.19E+06	1.35E+06																																																		
Acetyl-CoA	1.75E+04	1.47E+04	1.48E+04	1.32E+04	1.58E+04	1.90E+04	1.95E+04	2.28E+04	2.20E+04	2.11E+04	8.16E+03	8.63E+03	7.61E+03	7.69E+03	7.50E+03	1.26E+04	1.13E+04	1.15E+04	9.20E+03	1.11E+04	5.60E+04	4.17E+04	5.70E+04	6.28E+04	7.92E+04	1.98E+05	1.65E+05	1.90E+05	1.78E+05	1.78E+05	6.73E+03	1.10E+04	9.08E+03	6.32E+03	6.80E+03	1.31E+04	1.41E+04	1.36E+04	1.33E+04	1.30E+04	1.41E+05	1.13E+05	1.15E+05	1.43E+05	1.48E+05	2.64E+05	2.48E+05	2.54E+05	2.62E+05	3.16E+05																																																		
Glyceraldehyde 3-phosphate	4.34E+04	3.00E+04	3.96E+04	3.63E+04	2.52E+04	6.44E+04	8.19E+04	5.99E+04	6.09E+04	5.92E+04	1.43E+04	2.31E+04	1.74E+04	1.88E+04	2.30E+04	2.52E+04	2.16E+04	2.91E+04	2.95E+04	2.83E+04	8.85E+04	9.22E+04	7.29E+04	9.50E+04	8.27E+04	9.37E+05	1.00E+06	1.00E+06	8.13E+05	1.06E+06	1.31E+04	1.66E+04	1.67E+04	1.59E+04	1.38E+04	2.62E+04	2.18E+04	2.43E+04	2.43E+04	2.44E+04	6.95E+04	7.05E+04	3.58E+04	5.47E+04	7.38E+04	6.37E+05	6.62E+05	7.59E+05	5.71E+05	8.18E+05																																																		
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	m/z	PubChem ID	KEGG_ID
Glucose 6-phosphate	259	5958	C00668 
Fructose 6-phosphate	259	69507	C00085 
Fructose 1,6-bisphosphate	339	10267	C05378 
Glyceraldehyde 3-phosphate	169	439168	C00118   
Phosphoenolpyruvate	167	1005	C00074
Pyruvate	87	107735	C00022
Lactate	89	91435	C00186
Alanine	88	5950	C00041
Acetyl-CoA	808	444493	C00024
Ribulose 5-phosphate	229	439184	C00199
Ribose 5-phosphate	229	439167	C00117
METABOLITES_END
#END