#METABOLOMICS WORKBENCH jamiesop_20231023_092235 DATATRACK_ID:4415 STUDY_ID:ST002943 ANALYSIS_ID:AN004828 PROJECT_ID:PR001830 VERSION 1 CREATED_ON October 25, 2023, 5:01 pm #PROJECT PR:PROJECT_TITLE Targeted analyses of microbial metabolism for the XMaS clinical trial PR:PROJECT_SUMMARY The Xanthohumol microbiome and signature (XMaS) study in healthy adults was a PR:PROJECT_SUMMARY phase I, triple-masked, placebo-controlled clinical trial in healthy adults PR:PROJECT_SUMMARY investigating effects of a natural product supplement of gut microbiome PR:PROJECT_SUMMARY composition and metabolism. Xanthohumol, a flavonoid from the hops plant or PR:PROJECT_SUMMARY placebo was administered to 27 healthy adults daily for eight weeks. Fecal, PR:PROJECT_SUMMARY plasma, and 24-hr urine collections were obtained from participants at baseline PR:PROJECT_SUMMARY and 2-week intervals. The main objective was to examine clinical safety and PR:PROJECT_SUMMARY subjective tolerability of xanthohumol compared to placebo. Additional aims were PR:PROJECT_SUMMARY to monitor biomarkers of inflammation, gut permeability, bile acid metabolism, PR:PROJECT_SUMMARY short-chain fatty acids, and metabolism of xanthohumol. PR:INSTITUTE Oregon State University PR:LAST_NAME Jamieson PR:FIRST_NAME Paige PR:ADDRESS 307 LINUS PAULING SCIENCE CTR PR:EMAIL jamiesop@oregonstate.edu PR:PHONE 5417379804 #STUDY ST:STUDY_TITLE Targeted bile acid analysis for the XMaS trial. ST:STUDY_SUMMARY The Xanthohumol microbiome and signature (XMaS) study in healthy adults was a ST:STUDY_SUMMARY phase I, triple-masked, placebo-controlled clinical trial in healthy adults ST:STUDY_SUMMARY investigating effects of a natural product supplement of gut microbiome ST:STUDY_SUMMARY composition and metabolism. Xanthohumol, a flavonoid from the hops plant or ST:STUDY_SUMMARY placebo was administered to 27 healthy adults daily for eight weeks. Fecal, ST:STUDY_SUMMARY plasma, and 24-hr urine collections were obtained from participants at baseline ST:STUDY_SUMMARY and 2-week intervals. The main objective of this study was to quantify fecal ST:STUDY_SUMMARY bile acids from participants from the XMaS study. ST:INSTITUTE Oregon State University ST:LAST_NAME Jamieson ST:FIRST_NAME Paige ST:ADDRESS 307 LINUS PAULING SCIENCE CTR ST:EMAIL jamiesop@oregonstate.edu ST:PHONE 5417379804 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS XMaS101 s101-1 time_weeks:0 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS101 s101-2 time_weeks:2 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS101 s101-3 time_weeks:4 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS101 s101-4 time_weeks:6 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS101 s101-5 time_weeks:8 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS102 s102-1 time_weeks:0 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS102 s102-2 time_weeks:2 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS102 s102-3 time_weeks:4 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS102 s102-4 time_weeks:6 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS102 s102-5 time_weeks:8 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS103 s103-1 time_weeks:0 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS103 s103-2 time_weeks:2 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS103 s103-3 time_weeks:4 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS103 s103-4 time_weeks:6 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS103 s103-5 time_weeks:8 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS104 s104-1 time_weeks:0 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS104 s104-2 time_weeks:2 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS104 s104-3 time_weeks:4 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS104 s104-4 time_weeks:6 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS104 s104-5 time_weeks:8 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS105 s105-1 time_weeks:0 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS105 s105-2 time_weeks:2 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS105 s105-3 time_weeks:4 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS105 s105-4 time_weeks:6 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS105 s105-5 time_weeks:8 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS106 s106-1 time_weeks:0 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS106 s106-2 time_weeks:2 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS106 s106-3 time_weeks:4 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS106 s106-4 time_weeks:6 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS106 s106-5 time_weeks:8 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS107 s107-1 time_weeks:0 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS107 s107-2 time_weeks:2 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS107 s107-3 time_weeks:4 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS107 s107-4 time_weeks:6 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS107 s107-5 time_weeks:8 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS109 s109-1 time_weeks:0 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS109 s109-2 time_weeks:2 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS109 s109-3 time_weeks:4 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS109 s109-4 time_weeks:6 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS109 s109-5 time_weeks:8 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS110 s110-1 time_weeks:0 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS110 s110-2 time_weeks:2 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS110 s110-3 time_weeks:4 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS110 s110-4 time_weeks:6 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS110 s110-5 time_weeks:8 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS111 s111-1 time_weeks:0 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS111 s111-2 time_weeks:2 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS111 s111-3 time_weeks:4 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS111 s111-4 time_weeks:6 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS111 s111-5 time_weeks:8 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS112 s112-1 time_weeks:0 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS112 s112-2 time_weeks:2 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS112 s112-3 time_weeks:4 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS112 s112-4 time_weeks:6 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS112 s112-5 time_weeks:8 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS113 s113-1 time_weeks:0 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS113 s113-2 time_weeks:2 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS113 s113-3 time_weeks:4 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS113 s113-4 time_weeks:6 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS113 s113-5 time_weeks:8 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS114 s114-1 time_weeks:0 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS114 s114-2 time_weeks:2 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS114 s114-3 time_weeks:4 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS114 s114-4 time_weeks:6 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS114 s114-5 time_weeks:8 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS115 s115-1 time_weeks:0 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS115 s115-2 time_weeks:2 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS115 s115-3 time_weeks:4 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS115 s115-4 time_weeks:6 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS115 s115-5 time_weeks:8 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS116 s116-1 time_weeks:0 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS116 s116-2 time_weeks:2 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS116 s116-3 time_weeks:4 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS116 s116-4 time_weeks:6 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS116 s116-5 time_weeks:8 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS117 s117-1 time_weeks:0 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS117 s117-2 time_weeks:2 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS117 s117-3 time_weeks:4 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS117 s117-4 time_weeks:6 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS117 s117-5 time_weeks:8 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS118 s118-1 time_weeks:0 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS118 s118-2 time_weeks:2 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS118 s118-3 time_weeks:4 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS118 s118-4 time_weeks:6 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS118 s118-5 time_weeks:8 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS119 s119-1 time_weeks:0 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS119 s119-2 time_weeks:2 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS119 s119-3 time_weeks:4 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS119 s119-4 time_weeks:6 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS119 s119-5 time_weeks:8 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS121 s121-1 time_weeks:0 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS121 s121-2 time_weeks:2 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS121 s121-3 time_weeks:4 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS121 s121-4 time_weeks:6 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS121 s121-5 time_weeks:8 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS122 s122-1 time_weeks:0 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS122 s122-2 time_weeks:2 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS122 s122-3 time_weeks:4 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS122 s122-4 time_weeks:6 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS122 s122-5 time_weeks:8 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS123 s123-1 time_weeks:0 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS123 s123-2 time_weeks:2 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS123 s123-3 time_weeks:4 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS123 s123-4 time_weeks:6 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS123 s123-5 time_weeks:8 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS124 s124-1 time_weeks:0 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS124 s124-2 time_weeks:2 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS124 s124-3 time_weeks:4 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS124 s124-4 time_weeks:6 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS124 s124-5 time_weeks:8 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS126 s126-1 time_weeks:0 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS126 s126-2 time_weeks:2 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS126 s126-3 time_weeks:4 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS126 s126-4 time_weeks:6 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS126 s126-5 time_weeks:8 | intervention:control SUBJECT_SAMPLE_FACTORS XMaS127 s127-1 time_weeks:0 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS127 s127-2 time_weeks:2 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS127 s127-3 time_weeks:4 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS127 s127-4 time_weeks:6 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS127 s127-5 time_weeks:8 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS129 s129-1 time_weeks:0 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS129 s129-2 time_weeks:2 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS129 s129-3 time_weeks:4 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS129 s129-4 time_weeks:6 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS129 s129-5 time_weeks:8 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS130 s130-1 time_weeks:0 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS130 s130-2 time_weeks:2 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS130 s130-3 time_weeks:4 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS130 s130-4 time_weeks:6 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS130 s130-5 time_weeks:8 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS131 s131-1 time_weeks:0 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS131 s131-2 time_weeks:2 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS131 s131-3 time_weeks:4 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS131 s131-4 time_weeks:6 | intervention:treated SUBJECT_SAMPLE_FACTORS XMaS131 s131-5 time_weeks:8 | intervention:treated #COLLECTION CO:COLLECTION_SUMMARY Fecal samples were collected longitudinally at 2-week intervals during the CO:COLLECTION_SUMMARY 8-week study. Samples were kept on ice immediately after collection and CO:COLLECTION_SUMMARY snap-frozen, then stored in -80 freezers until processing. CO:SAMPLE_TYPE Feces #TREATMENT TR:TREATMENT_SUMMARY Participants were randomly allocated to intervention arms to receive either 24 TR:TREATMENT_SUMMARY mg or a rice protein placebo capsule daily for 8 weeks. Clinic check-in and TR:TREATMENT_SUMMARY sample collections occurred at baseline and 2-week intervals until study TR:TREATMENT_SUMMARY close-out (week 8). #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Fecal samples were dried using vacuum desiccator and 70% methanol:water (1:4 SP:SAMPLEPREP_SUMMARY w/v) was added for fecal homogenate. Samples were centrifuged and supernatant SP:SAMPLEPREP_SUMMARY was collected. 10 uL of fecal supernatant, 10 uL of cholic acid-D4 internal SP:SAMPLEPREP_SUMMARY standard (1 ug/mL in ethanol) and 80 uL of 50% methanol:water were mixed and SP:SAMPLEPREP_SUMMARY added to a autosampler vial. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Shimadzu 20AD CH:COLUMN_NAME Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) CH:SOLVENT_A water with 0.1% formic acid (v/v) CH:SOLVENT_B acetonitrile with 0.1% formic acid (v/v) CH:FLOW_GRADIENT 0 to 1 min, 35% B; 10 min, 60% B; 10.5 min, 99% B; 13 min, 99% B; 13.5 to 17 CH:FLOW_GRADIENT min, 35% B CH:FLOW_RATE 0.25 mL/min CH:COLUMN_TEMPERATURE 40 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME ABI Sciex 4000 QTrap MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS Metabolites were confirmed by matching m/z values, retention times and product MS:MS_COMMENTS ions of SRM transitions with those of reference standards #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS ng/g MS_METABOLITE_DATA_START Samples s101-1 s101-2 s101-3 s101-4 s101-5 s102-1 s102-2 s102-3 s102-4 s102-5 s103-1 s103-2 s103-3 s103-4 s103-5 s104-1 s104-2 s104-3 s104-4 s104-5 s105-1 s105-2 s105-3 s105-4 s105-5 s106-1 s106-2 s106-3 s106-4 s106-5 s107-1 s107-2 s107-3 s107-4 s107-5 s109-1 s109-2 s109-3 s109-4 s109-5 s110-1 s110-2 s110-3 s110-4 s110-5 s111-1 s111-2 s111-3 s111-4 s111-5 s112-1 s112-2 s112-3 s112-4 s112-5 s113-1 s113-2 s113-3 s113-4 s113-5 s114-1 s114-2 s114-3 s114-4 s114-5 s115-1 s115-2 s115-3 s115-4 s115-5 s116-1 s116-2 s116-3 s116-4 s116-5 s117-1 s117-2 s117-3 s117-4 s117-5 s118-1 s118-2 s118-3 s118-4 s118-5 s119-1 s119-2 s119-3 s119-4 s119-5 s121-1 s121-2 s121-3 s121-4 s121-5 s122-1 s122-2 s122-3 s122-4 s122-5 s123-1 s123-2 s123-3 s123-4 s123-5 s124-1 s124-2 s124-3 s124-4 s124-5 s126-1 s126-2 s126-3 s126-4 s126-5 s127-1 s127-2 s127-3 s127-4 s127-5 s129-1 s129-2 s129-3 s129-4 s129-5 s130-1 s130-2 s130-3 s130-4 s130-5 s131-1 s131-2 s131-3 s131-4 s131-5 Factors time_weeks:0 | intervention:treated time_weeks:2 | intervention:treated time_weeks:4 | intervention:treated time_weeks:6 | intervention:treated time_weeks:8 | intervention:treated time_weeks:0 | intervention:treated time_weeks:2 | intervention:treated time_weeks:4 | intervention:treated time_weeks:6 | intervention:treated time_weeks:8 | intervention:treated time_weeks:0 | intervention:treated time_weeks:2 | intervention:treated time_weeks:4 | intervention:treated time_weeks:6 | intervention:treated time_weeks:8 | intervention:treated time_weeks:0 | intervention:control time_weeks:2 | intervention:control time_weeks:4 | intervention:control time_weeks:6 | intervention:control time_weeks:8 | intervention:control time_weeks:0 | intervention:treated time_weeks:2 | intervention:treated time_weeks:4 | intervention:treated time_weeks:6 | intervention:treated time_weeks:8 | intervention:treated time_weeks:0 | intervention:control time_weeks:2 | intervention:control time_weeks:4 | intervention:control time_weeks:6 | intervention:control time_weeks:8 | intervention:control time_weeks:0 | intervention:control time_weeks:2 | intervention:control time_weeks:4 | intervention:control time_weeks:6 | intervention:control time_weeks:8 | intervention:control time_weeks:0 | intervention:control time_weeks:2 | intervention:control time_weeks:4 | intervention:control time_weeks:6 | intervention:control time_weeks:8 | intervention:control time_weeks:0 | intervention:control time_weeks:2 | intervention:control time_weeks:4 | intervention:control time_weeks:6 | intervention:control time_weeks:8 | intervention:control time_weeks:0 | intervention:treated time_weeks:2 | intervention:treated time_weeks:4 | intervention:treated time_weeks:6 | intervention:treated time_weeks:8 | intervention:treated time_weeks:0 | intervention:control time_weeks:2 | intervention:control time_weeks:4 | intervention:control time_weeks:6 | intervention:control time_weeks:8 | intervention:control time_weeks:0 | intervention:treated time_weeks:2 | intervention:treated time_weeks:4 | intervention:treated time_weeks:6 | intervention:treated time_weeks:8 | intervention:treated time_weeks:0 | intervention:control time_weeks:2 | intervention:control time_weeks:4 | intervention:control time_weeks:6 | intervention:control time_weeks:8 | intervention:control time_weeks:0 | intervention:treated time_weeks:2 | intervention:treated time_weeks:4 | intervention:treated time_weeks:6 | intervention:treated time_weeks:8 | intervention:treated time_weeks:0 | intervention:treated time_weeks:2 | intervention:treated time_weeks:4 | intervention:treated time_weeks:6 | intervention:treated time_weeks:8 | intervention:treated time_weeks:0 | intervention:control time_weeks:2 | intervention:control time_weeks:4 | intervention:control time_weeks:6 | intervention:control time_weeks:8 | intervention:control time_weeks:0 | intervention:control time_weeks:2 | intervention:control time_weeks:4 | intervention:control time_weeks:6 | intervention:control time_weeks:8 | intervention:control time_weeks:0 | intervention:control time_weeks:2 | intervention:control time_weeks:4 | intervention:control time_weeks:6 | intervention:control time_weeks:8 | intervention:control time_weeks:0 | intervention:control time_weeks:2 | intervention:control time_weeks:4 | intervention:control time_weeks:6 | intervention:control time_weeks:8 | intervention:control time_weeks:0 | intervention:control time_weeks:2 | intervention:control time_weeks:4 | intervention:control time_weeks:6 | intervention:control time_weeks:8 | intervention:control time_weeks:0 | intervention:treated time_weeks:2 | intervention:treated time_weeks:4 | intervention:treated time_weeks:6 | intervention:treated time_weeks:8 | intervention:treated time_weeks:0 | intervention:treated time_weeks:2 | intervention:treated time_weeks:4 | intervention:treated time_weeks:6 | intervention:treated time_weeks:8 | intervention:treated time_weeks:0 | intervention:control time_weeks:2 | intervention:control time_weeks:4 | intervention:control time_weeks:6 | intervention:control time_weeks:8 | intervention:control time_weeks:0 | intervention:treated time_weeks:2 | intervention:treated time_weeks:4 | intervention:treated time_weeks:6 | intervention:treated time_weeks:8 | intervention:treated time_weeks:0 | intervention:treated time_weeks:2 | intervention:treated time_weeks:4 | intervention:treated time_weeks:6 | intervention:treated time_weeks:8 | intervention:treated time_weeks:0 | intervention:treated time_weeks:2 | intervention:treated time_weeks:4 | intervention:treated time_weeks:6 | intervention:treated time_weeks:8 | intervention:treated time_weeks:0 | intervention:treated time_weeks:2 | intervention:treated time_weeks:4 | intervention:treated time_weeks:6 | intervention:treated time_weeks:8 | intervention:treated GCA 7.454333081 22.9724208 10.19789428 45.11659592 273.0437619 290.0212805 54.73473614 1894.507895 114.8924529 378.6217405 28.83945164 36.07018861 56.16618238 85.27286553 183.3840211 143.8132162 31.2893926 507.0856268 44.37901329 84.85546259 64.45529472 100.1250129 22.15308439 111.7107431 22.01544433 61.33986675 89.00066043 93.99440481 18.37112504 91.7240329 33.00691478 12.35575815 40.33882869 70.47878292 28.69696944 82.23361464 131.2957512 51.01411083 198.9969452 40.7475483 71.7376883 51.94620346 98.36673335 28.04697161 53.68450222 462.0319645 99.27503871 1430.654778 153.3403974 653.5539935 35.3690113 95.55553661 44.21587441 58.67710034 83.52919558 98.28149415 46.99507459 15.57597824 64.60921075 218.2851662 170.3151411 211.718152 114.4097679 160.5346052 195.8898652 241.7078933 308.301842 548.1596841 1761.30131 65.52387408 32.52095151 19.28803157 18.95476592 115.8916982 27.99008458 115.844115 15.58827515 90.24166849 56.56969711 31.74350048 26.86212622 125.8875701 86.55679048 12.54208577 23.29116642 317.2491263 155.417404 1132.821249 142.7296347 80.10254224 120.6508398 78.26782458 5.524763037 14.52701302 17.48540891 220.3040151 101.4972573 24.41433177 39.19207798 39.93321788 198.1335275 33.54969255 41.45129415 6.355922166 119.5066725 261.5680314 225.9962635 119.3684377 130.685703 94.96550093 7.895768473 14.16771474 15.82938533 29.90774936 38.63236206 109.7235495 194.6841302 569.0175413 731.1109136 801.7916917 73.50157247 55.54352683 51.57168364 145.1122624 8.792319497 268.2423892 42.84843845 110.2372377 21.63176662 5.76778442 71.24030429 79.73049556 65.84470357 38.44500588 33.13334464 GCDCA 15.45716522 26.86926786 19.82361158 71.87970854 167.5070327 508.5737066 91.48529017 4091.478827 164.1806146 1059.058887 50.96952358 44.73869931 64.47773047 80.34557497 177.6365378 362.911905 70.02784071 1543.140086 82.40192939 265.3002663 64.58883566 175.9893308 44.48558082 229.0271518 69.6568574 104.5330673 82.68695167 137.006869 22.67645694 95.66619062 25.30716882 12.95955233 23.51654745 60.36615429 31.55168969 120.4387851 269.2045191 103.9757225 578.0227802 116.7751645 76.47715065 39.86642948 110.7711888 30.95036732 57.31491351 358.0751974 104.8669414 1133.101834 528.8491778 589.8128075 35.92075011 81.4440811 48.25598203 50.24734389 90.30803914 135.8617508 30.35546607 24.88064032 52.5535515 102.1107775 65.00889115 106.0606024 66.28921542 89.56755634 106.5681264 165.3179016 197.3194586 225.7347501 517.189667 108.7231818 26.97197598 20.11928999 19.56552597 64.660812 26.80455439 167.5263091 26.01253821 115.7518803 84.82597721 1032.232602 116.3107278 202.996233 94.50367232 23.73270475 32.67877544 273.3418808 95.02870175 1126.400345 224.7148405 138.0041461 219.8774631 95.08592565 14.33363752 26.02560371 24.93259993 110.5501297 66.10270467 36.58284754 65.36032575 49.25192415 131.5337284 26.55444902 29.61331003 7.658131015 55.39839737 102.5344139 114.08363 69.46105048 64.19010023 58.06554183 22.48609275 20.43416803 28.6938777 32.23364425 86.06683429 58.94100808 93.49967972 158.8085068 285.2809663 313.7913296 121.8737699 60.96670585 70.31714718 161.4665507 22.20285564 193.8319596 40.68871945 83.83032878 51.58761968 12.34645584 148.8847958 153.1516732 139.7956229 81.19662605 37.71777428 CA 38.94857586 34.44555212 50.4036173 851.6270348 200.7054575 1330.617574 1078.197692 5272.528999 2861.369071 2141.270441 23.36882012 657.4926877 34.8333188 25.70897982 170.2637697 3061.290812 32.73012562 17113.87732 257.5232939 406.4308373 33.0065716 494.9912959 20.56651519 66.63221338 135.4629832 62.75514311 187.2800634 128.1561397 38.9723654 325.1224303 28.91836959 17.65088667 23.44197463 31.12756064 21.53581981 149.1296571 645.5918171 80.92804194 180.0344559 171.2594951 67.66691633 12.76176268 64.94993978 21.44386664 24.54216567 19940.36163 194.2195153 590.3729687 4775.719662 2685.269364 43.94146607 47.99770597 23.18653294 15.39971584 24.68353389 1281.961164 83.98593089 110.1145712 69.13183976 135.944329 60.64226906 113.2097312 60.84898482 83.69547483 111.3764094 98.09821222 73.49229113 96.16782349 432.9070361 70.58051078 14.86777563 34.40639732 105.625244 66.49292029 16.33721556 745.7798874 34.99738723 421.9070017 1977.874315 579.6102188 535.7052723 1805.417303 158.6304271 59.03633572 25.21738603 13650.4964 1374.185575 6301.270019 1402.742587 6322.927121 1616.933797 570.9574142 38.31222475 51.97058859 94.16802281 190.8784029 126.5364493 40.85261389 62.06448716 84.57463211 89.46807023 29.34311277 30.46362733 9.938472059 43.2353113 58.22373295 79.01831116 38.09480255 67.40825031 69.96405049 47.41648187 26.05361371 29.62245781 50.57025513 59.5944566 164.2856194 352.0855178 50.37813888 148.3451389 782.1277928 65.46302072 66.99360525 91.84790919 175.8181688 26.69844457 118.8097663 44.03034862 91.42340074 37.26993305 13.93189998 1144.961924 205.3718702 201.2764853 102.3385957 25.95548237 CDCA 0 0 0 1355.087679 329.7399897 2531.247169 1925.05354 17457.70136 5127.223624 3878.776205 0 830.624948 116.8967442 0 143.9478173 6059.840909 20.77255906 31211.23487 315.963017 482.3516401 0 529.1451854 0 843.4771844 0 112.6848555 188.7810678 209.4255433 23.51749155 1242.926542 252.3472916 0 0 0 52.89702209 315.6419834 1618.958988 99.3811195 883.064677 659.6413793 166.9840513 25.5964797 127.0513349 28.72165958 65.3091667 15122.79889 206.9231098 435.2233263 5261.26513 4075.806345 76.41252072 74.6308342 52.92693647 35.08718882 40.94622397 1891.568508 35.0731718 0 22.47326303 40.98176063 30.51620064 61.64198167 36.49570299 277.7649219 199.6932828 145.8339657 0 67.89969967 134.2672404 592.6979528 67.56563533 221.7174617 63.05822596 69.64115528 35.96286477 1280.733768 20.78431738 1357.330289 3916.06776 4655.943979 871.733383 4300.023701 111.2287303 55.16947707 29.12089831 12760.67238 830.7799959 6999.110269 2712.963753 9644.267447 1072.937297 1169.394624 32.83985176 105.9292851 130.475911 173.8689098 628.334437 85.31430061 102.6936472 90.10442441 0 0 0 0 0 36.46464385 83.18267837 22.06951029 58.20495052 69.28732111 0 0 0 0 0 193.6119956 761.2756511 172.3660767 361.495215 1048.39228 127.6453856 83.15748635 118.8830265 654.8514277 267.2662128 156.2391522 49.1949183 173.7247889 66.10571667 0 2094.910294 415.3069688 1779.239519 397.6611572 34.91265999 LCA 7069.072442 3929.59507 8639.613695 3421.36677 6801.623582 5580.933291 8524.860447 11574.99943 9461.071763 12780.05958 5988.668146 4190.897658 5109.137951 3928.880319 5762.552431 24103.88011 7676.335714 13106.94748 14389.69536 15123.13103 5917.6287 5129.027594 4959.853884 9430.812954 13555.2065 10754.80871 10181.85666 11153.65809 10043.04071 9328.299408 9921.742405 5536.622064 4427.249895 8289.72551 5284.262183 11778.00602 11778.00822 9729.554919 13136.30841 6616.615926 3539.808373 3512.188275 3030.700536 3525.25609 5768.905932 6950.297354 3886.310121 4430.014172 6008.582469 6984.318139 4315.4113 5191.815574 3951.226032 5506.487414 6563.220828 7757.521443 3130.600167 6021.25754 3097.288343 4490.659513 5804.295254 2074.326175 4588.12181 4558.105862 1351.243245 4467.421201 6558.523524 2854.031002 852.4800469 2221.654672 3448.388711 2358.804701 1037.711234 624.6880203 1025.743548 3416.297542 11511.76163 6193.299789 5938.658683 5058.737579 8087.894711 5914.883342 2606.013282 5904.765308 2512.859525 2857.740359 2763.598803 5681.31716 14253.31002 6531.298504 1359.942493 13311.20463 6237.013931 5636.986307 4812.73484 889.1315897 2738.327628 6923.028384 600.7701076 4716.922902 10126.12371 5701.424509 5026.871447 3927.969268 3707.032407 5232.070688 3370.351382 2995.655413 2921.124315 1174.329015 7816.154693 3557.359003 6056.983911 3453.190784 8962.076587 1206.179726 3395.684313 1986.078072 850.2280669 2037.049299 8697.198894 6599.138429 6177.016185 7036.537369 7544.744124 1314.873816 1418.845738 4504.81548 2755.121681 2320.10942 3635.233158 1288.856566 5444.073684 2203.641718 4974.569714 DCA 4825.495382 3826.004274 5938.012393 6419.653641 9197.41279 9529.872394 8054.242284 12880.50259 11600.68122 10965.72383 2232.024617 2943.738261 1841.265448 997.7408889 4122.782021 14165.91336 7707.94951 22616.21263 11018.90385 14496.73803 6788.95316 4375.555323 3458.973368 7634.189331 19346.92634 6786.60161 8060.140657 10950.45254 7048.521134 12246.60328 5744.556379 2136.776088 2201.114009 3524.005756 4389.419198 7624.463866 8614.473501 5541.558698 7972.736468 5840.31912 1461.565771 1162.677068 1812.786718 1024.967495 3364.33924 17958.2081 4626.185822 8015.922103 13189.65635 10461.39031 906.5144937 919.4459331 1596.182375 1313.670485 2852.15603 10746.37713 5587.697531 8423.622431 3496.580239 4259.134327 5716.671899 1670.705615 5888.26188 5238.139585 1657.536171 4000.557292 4076.298679 3558.149937 2886.867324 1392.312162 1738.855824 2082.081185 451.2261589 146.6821084 310.9888908 3435.6763 9257.912359 6087.364913 6855.654074 6443.235028 8191.72177 8227.856871 3270.833377 4295.109878 1767.405213 8469.394861 10341.61901 14058.22103 11539.05649 11902.25007 9882.524479 12578.85504 4534.25973 4430.933419 6302.826054 3541.912029 5395.599196 6048.626243 1828.531917 5011.358014 2939.61314 3153.801076 1302.693145 786.5688293 2452.740373 3539.999188 4244.358833 2259.094819 3433.235011 4097.572922 4803.392728 1926.245574 5066.978871 4314.021197 7631.26246 363.3194234 1519.510184 393.806348 322.6681987 1200.285643 4959.24517 4625.68802 5934.898508 6925.613825 4275.339241 4404.136451 3031.527895 4337.621889 4045.18336 2837.249371 5443.671453 3933.94092 7177.254342 5299.491024 6515.956065 GDCA 35.99115276 25.27785773 30.21091216 84.55616306 227.0037108 273.8694554 159.9172853 1413.450822 99.4919528 581.8011985 30.8964375 17.25398439 15.74660487 14.05889379 45.49531608 238.9903835 53.36025537 688.539471 189.7106976 313.3633073 99.12144882 177.7756666 82.06734224 214.9732938 174.9011727 137.9816324 215.3298723 137.359933 73.08485879 116.0264287 26.73227472 10.77898814 15.10613838 40.23599661 23.19428815 126.7725017 165.7074832 65.80313203 358.1720486 64.47517503 24.60793857 8.667818986 39.3784867 11.44449463 32.72660531 166.830735 69.96366389 409.2824822 498.6868273 259.9461338 8.274430323 22.62475081 12.20525065 10.0649798 28.91721574 411.6043277 68.79174832 42.17244352 51.08185704 128.7149117 49.74105352 35.2943046 48.95106113 33.84079407 33.31523899 53.0939923 81.51133737 58.41412001 253.4202758 47.26647939 8.879792 11.87264549 2.308437661 2.58045014 1.870877751 47.77737331 66.94766928 43.35671962 60.02235681 89.94931891 68.2798092 143.4261739 58.68736795 23.8981638 21.53606829 83.98016804 89.13853579 395.6254429 507.2952581 45.96401147 236.139906 160.8705709 32.98764631 48.84016275 55.44644329 68.57548907 52.93908327 84.20940426 25.74086606 35.81769791 46.40591002 34.16794812 19.8229584 4.581427267 39.43201021 65.39200803 75.4906917 34.152121 77.94211551 60.94012571 28.27901129 13.51385891 35.80386898 19.73047315 198.5091352 5.356358634 22.73884386 43.55545719 27.36094649 75.53066786 98.98893658 89.58105049 106.9517335 143.9115883 31.3279732 87.15034322 18.60770613 57.95127959 31.62480308 21.47412785 75.11421725 76.67322095 131.2578747 51.28844463 77.55047824 GLCA 0 0 0 645279.8471 157019.0427 1205355.795 916692.1618 8313191.126 2441535.059 1847036.288 0 395535.6895 55665.11626 0 68546.57966 2885638.528 9891.694789 14862492.79 150458.5795 229691.2572 0 251973.8978 0 401655.8021 0 53659.45502 89895.74659 99726.44917 11198.8055 591869.7817 120165.3769 0 0 0 25189.05814 150305.7064 770932.8516 47324.34262 420506.9891 314114.9425 79516.21489 12188.79986 60500.63565 13676.98075 31099.60319 7201332.803 98534.81418 207249.203 2505364.347 1940860.164 36386.91463 35538.49248 25203.30308 16708.18515 19498.20189 900746.9087 16701.51038 0 10701.55382 19515.12411 14531.52411 29353.3246 17378.90618 132269.0105 95092.03942 69444.74558 0 32333.19032 63936.78114 282237.1204 32174.11206 105579.7437 30027.72665 33162.4549 17125.1737 609873.223 9897.293991 646347.7568 1864794.171 2217116.18 415111.1348 2047630.334 52966.06205 26271.17956 13867.09443 6076510.658 395609.5218 3332909.652 1291887.501 4592508.308 510922.5223 556854.5829 15638.02465 50442.5167 62131.38621 82794.71896 299206.8748 40625.85743 48901.73675 42906.86877 0 0 0 0 0 17364.11612 39610.79922 10509.29062 27716.64311 32993.96243 0 0 0 0 0 92196.18836 362512.2148 82079.08414 172140.5786 499234.419 60783.51694 39598.80303 56610.96499 311834.0132 127269.6252 74399.59627 23426.15157 82726.08995 31478.9127 0 997576.3304 197765.2232 847256.9139 189362.4558 16625.07619 TCA 3.162617732 6.425811144 5.385548675 23.92098513 36.74746885 89.92012145 8.953790948 510.9720831 25.73640802 190.4560711 6.167799775 18.84965483 11.67921777 15.14229178 26.74219118 17.95080344 5.89197782 212.234489 10.60173604 14.69420032 10.6491378 17.92774407 2.913287915 16.80593383 2.590181251 9.330136993 14.70373614 13.46506955 4.854031705 15.21765872 5.446289309 3.284940601 15.73732269 13.03863884 9.249461985 8.50407602 21.7597665 8.808861468 14.62458486 16.551041 19.05767197 18.8989345 25.78573041 4.474610641 10.90873783 406.3075463 22.58704318 179.5220858 20.23046783 95.88672092 17.86202677 34.9800507 7.23590887 10.52825751 23.84268593 14.28523178 12.78635976 4.76691285 18.01150837 68.39510336 29.29179717 54.87208121 28.20555733 50.79586439 33.4260201 74.80507727 51.73512778 119.5963888 421.2485475 19.81421019 4.476056736 5.904040082 7.861656167 26.64542239 5.541163763 54.15809132 9.758243478 23.98263976 16.30327539 16.8936797 18.47124716 35.45641525 23.97160215 3.239600283 4.349993193 3654.524153 76.07986691 466.021536 32.28397132 45.60784312 48.54664129 122.5672181 4.732286373 15.04235586 19.55674678 61.27561172 20.68356618 6.320495479 9.567263913 11.57625382 16.82908392 3.491895101 5.229145387 0.972164444 13.62516794 30.62105633 39.99612894 11.73873743 16.47495816 10.03315571 2.052727739 3.596402676 4.000983229 9.160162396 7.36788305 42.97970933 40.61630675 143.6939637 193.5971087 278.9421136 5.702692615 2.98095495 1.894931056 9.77750706 1.457701788 12.75576162 3.018674018 10.57272194 1.596976339 0.952772961 34.42233134 20.35695326 23.17019303 9.672122926 3.317760612 TCDCA 9.021239789 10.3466743 11.35564791 60.44626794 37.09641555 196.5286285 21.47808629 1945.810221 52.9012248 530.1486585 15.07533793 233.5459706 24.15334201 16.08452075 35.79875106 57.46024623 15.56481913 581.8402957 23.24961523 70.06218338 14.49633516 15.45103001 6.310210782 43.40430499 5.599835276 23.88908048 21.40501447 24.42418412 6.238127586 20.09641709 6.158096968 4.272544418 9.923550116 15.89569245 9.320274469 17.57466379 51.20160629 20.4861152 70.10685103 32.64139947 23.21012071 19.24929541 42.77255059 6.07505488 16.69824914 142.0173266 35.63067804 231.0813825 155.3751173 168.480055 23.71360313 47.97338628 12.02411338 15.83029339 41.45615609 29.22035602 15.28170441 10.30826382 19.43678207 44.93039816 16.54503391 30.31499123 25.86867657 32.32658138 28.80980334 67.02895598 38.96654753 60.90715423 186.135642 35.61377048 5.148565886 5.933794707 6.246827912 16.71946404 5.17013316 101.1094106 13.80058728 55.66229595 41.97988792 551.6380743 97.58648173 116.0842587 35.65458754 8.29052228 6.706210332 1013.627927 64.39883352 976.1303004 67.99489953 186.6057146 232.3692165 211.4868818 11.72364749 38.26476343 37.78139639 51.2331117 24.36657982 13.71862947 24.82063318 23.2172639 14.42216225 3.739517335 3.703488576 0.799288983 9.157894302 15.32195817 27.96034721 11.86017839 12.50671895 8.824025838 4.4969046 7.569154067 7.766487415 11.65796736 17.92043396 28.29364011 28.98450785 48.42983151 102.9901059 144.8411187 12.76150495 4.726504121 2.943899398 26.8063609 3.499983068 18.41291299 3.425854958 10.12627497 5.32477191 1.733606357 95.73068985 62.76922372 78.58599305 28.93828064 4.517098894 TLCA 0.925735798 2.422273386 1.099680531 2.978630764 1.999796227 7.779198124 3.126795506 32.50182629 6.249367871 13.10145419 0.627961446 1.555689671 2.233479112 0.402146773 1.818787257 19.32636376 1.255095223 32.41301875 4.12714279 3.865581295 17.84577733 2.387118152 8.947986074 12.77613231 0 3.222316088 2.04403115 3.953427967 1.016764813 2.669007627 8.540206765 0.510230876 0.490714568 1.101293184 1.135735638 1.620635939 6.192266635 2.679068101 6.480904111 7.042150499 1.876523589 1.039132327 1.772251589 0 0.837926887 11.86171176 1.357037847 4.902806827 9.43154321 22.5947099 2.069515525 2.888428688 1.690660487 1.8080883 4.638514164 2.145612632 1.358457352 2.501420186 2.931352758 1.503762137 0.848815859 0.90962684 0.673432692 0.9151495 0.981293025 6.910070365 1.569530465 1.835841506 4.285400396 8.693680668 0 0 0 0 0 8.525360621 94.10183768 7.401839843 6.478453613 18.82408144 3.845571639 13.27006331 0.891832676 2.750473963 1.385283698 55.29853682 7.280894365 32.24826496 25.28289984 23.6107829 4.920832216 17.069695 2.270954112 3.862459494 2.335073067 3.774991636 2.33792045 3.682752724 1.180538555 3.344909495 1.171584474 0.427443066 0 0 0 1.207740257 0.987499267 0.698413841 1.850029846 0 0 0 0.810334001 0 0.693149456 1.752918 5.672870969 4.802560909 2.014712729 9.648737949 1.692555464 0.687174926 0.484838099 1.664532047 0.980677857 0.722124381 0 0.442824082 0 0 9.094092012 4.31622354 13.57028376 3.489208702 0 TDCA 5.548983368 5.663129576 7.146636259 51.99252858 29.531486 107.5454513 15.63647937 347.6382727 36.92598906 252.4330683 5.267446757 15.67482887 4.252276901 1.608589674 6.357201935 64.87445396 7.106476101 197.9530267 11.12018334 32.48607053 45.16024679 10.83315877 11.32146602 35.17823719 5.21377652 21.10591523 15.00179279 17.78370275 5.735547521 13.95899841 9.677542642 1.975763192 3.686080132 4.382274429 2.967064708 5.461810552 16.85776812 6.362164162 26.88590359 11.60665996 5.930872942 2.1475145 10.5386192 1.407267532 6.291012996 109.8786397 6.639787177 52.14228898 66.37885385 50.14885018 4.623528851 7.929977904 2.860206857 3.038174451 7.568612854 17.90196793 10.29292004 8.346778322 14.70901179 43.07377615 3.927829872 7.255977374 4.472945421 6.277185952 6.459470022 22.36342253 11.13987884 14.3267413 72.89788354 16.16205133 0.639576982 0.829615515 0.626555286 1.019569795 0.423488901 35.02631326 263.5883664 22.55345994 23.72031291 53.85252468 19.89821656 72.22440878 16.26208686 5.066090074 3.648775233 358.834136 63.00891544 309.8668506 72.29043636 45.79011957 118.2675507 141.7783735 7.090693237 20.17325194 17.65468193 18.19963521 10.25280347 5.09324954 6.758467758 6.796693005 3.138615486 1.846989632 1.413541165 0.601064347 3.021772323 5.407177481 8.95091346 3.147591008 4.69841976 5.581313995 2.688638362 4.042415691 4.989699628 5.771839976 10.80718716 2.901285244 4.697818833 9.7135533 9.60886027 23.24605272 4.417592885 2.259420761 2.32908439 12.01477593 2.275188405 5.17118215 0.981079057 2.962412799 2.137842059 0.799170372 42.04034249 20.91841255 33.42884426 8.666952341 1.566071611 DHLCA 5028.473432 2259.476138 2075.461044 1006.582798 1141.872928 988.334992 1701.638753 1760.868341 1164.119216 1753.282527 3659.20007 3388.742719 2230.446281 1883.46143 2522.643171 1430.65462 3475.819487 4668.911312 3418.857588 2304.650369 1335.87491 3453.88509 1365.38616 4867.487628 10136.65317 2863.512011 1526.529008 1484.924251 3406.182614 1272.348003 4250.775732 2155.377301 1631.963792 2734.94531 1221.187179 2037.423659 2753.89701 2192.470872 1931.414354 2826.653381 2478.828246 917.1786755 1044.485929 840.2194161 943.2571531 0 868.9359576 643.8427133 1479.27187 1401.513172 1931.644611 3553.41506 1303.843427 4901.331534 3699.79966 1136.402861 4041.673507 3430.298793 1096.711164 1542.761842 1078.194419 868.5912076 1457.095892 868.9882374 1359.075801 3423.796371 1641.644351 896.7240932 915.1897569 2966.742137 2965.523088 2778.992989 2907.728354 2920.441999 997.1888135 2408.051062 2519.64372 1348.522246 999.3017562 721.2092894 1367.806082 1213.997687 1169.513905 759.7715933 1432.587874 0 1067.413749 0 1671.209791 0 833.5816698 1147.198362 2025.051309 1819.323705 1075.121277 531.5065685 1098.649324 1440.496509 811.1112394 1010.571022 4227.625084 2827.008636 4238.070784 5518.048137 1364.435082 2124.748085 1210.535732 1000.816194 1104.70956 692.4412203 1099.729774 2154.766824 2358.120119 3181.379567 2911.349831 601.016733 3968.727096 835.6512666 791.6123752 731.8820372 4870.162229 2203.984721 1970.806341 2364.120029 2758.568221 742.0054597 778.6048581 1038.776184 796.3848399 1093.949777 1159.674439 701.9268517 1052.947655 602.6278151 2200.108021 NDCA 8.942543469 5.278315112 7.55839294 5.907749827 11.70922234 4.782898488 6.606406475 5.605755203 5.111189336 5.345800476 2.594928471 3.043549656 1.912888077 0.929824235 3.999221093 12.21259407 10.02431097 9.162655628 7.670304035 8.987452385 2.904143094 2.231148763 1.214937304 4.203697073 2.637012694 4.297422019 9.14933065 8.501459594 7.215722762 9.872985634 7.692129064 6.173175522 3.868589923 3.553458181 5.897844482 3.083130427 4.330103796 4.782482017 5.660796455 2.012235933 2.562305985 1.65356488 2.52587796 1.311985664 2.64768742 5.093754174 1.000937626 6.612831554 3.540776963 3.006135755 2.548249012 2.45226881 2.257834206 1.392006933 2.279486134 15.94595695 7.141275946 10.48639163 2.238126407 3.608094479 5.852852409 2.16498229 5.943516042 3.592759409 3.907178951 5.678424389 4.204267669 1.47450608 3.860191264 1.617531976 1.026964346 2.164479682 0.728194837 0.413174736 1.506644688 0.647928051 3.589264059 1.57115813 1.521815577 1.274776101 5.438992402 3.675697283 2.497197053 2.700006551 0.971372969 3.857100042 5.225482919 8.689624079 7.136861255 4.826648116 18.06936973 16.26029472 9.958358459 8.159242598 25.95375982 4.535809256 5.280824706 9.604061507 2.410262953 7.813356183 3.768568893 3.321375115 0.913957273 1.191220621 4.314966376 3.93558629 5.614374563 4.598047569 5.51026238 8.494552969 4.002305768 2.17515949 3.167689886 1.271447581 6.611449923 0.138774411 1.289066508 0.164995938 0.392947896 1.122360091 3.103377344 6.5527903 5.719748276 6.085414359 4.448535903 2.885490759 1.934401873 2.912919413 2.319748833 1.514598019 3.571375386 2.587107789 4.882239653 4.136515653 5.081403176 7-KCDCA 97.26651054 96.04978504 63.81327218 238.0700759 76.27166983 194.5727805 562.7251514 1521.451839 424.1429319 254.7973135 0 125.8877509 109.6798627 0 0 562.9132289 42.28650968 1271.150933 78.3959664 170.5403086 0 59.34108723 0 64.85880572 56.07019252 0 0 0 0 105.3918512 89.18723758 113.1184595 0 0 0 57.49016156 280.4310586 80.0793766 107.8042911 39.80517522 0 0 0 0 0 1381.48881 66.62863855 0 391.3018682 218.3408553 74.9766999 104.8734541 102.1488861 90.65583991 45.9719899 203.9091037 120.0460921 93.5788948 25.82395136 27.7127819 29.33235345 32.13999687 56.10075011 41.56768264 37.49225335 23.61795382 23.63751724 12.95967094 36.43860401 103.6825739 7.74353585 0 0 0 35.40922272 108.9080258 0 118.8372429 542.4629842 183.3553609 150.6708626 173.6680911 23.86106691 35.85674046 32.65097151 142.2784578 200.1031593 673.4571254 357.6253833 336.4806991 386.5443304 244.7622968 71.27694284 72.58152601 63.10685575 28.09654982 31.11457559 0 18.78181593 0 0 0 0 0 0 0 0 0 0 0 0 0 0 39.36461199 0 46.72498669 220.6437742 79.17125438 86.03810664 131.1590891 105.758896 100.2786371 64.15954216 156.2876053 206.1479551 46.26510326 20.51479182 28.2016249 34.45922357 15.80109769 61.10134612 61.06012099 150.6758471 28.26313244 25.85408024 UDCA 218.8112751 118.9677909 160.5615606 0 215.4344564 0 195.4093794 0 0 0 65.47900763 144.7801739 94.3489736 58.25703057 106.6769591 309.3240966 118.2978438 0 192.1053838 273.8546348 89.72051849 0 52.72012127 143.5921137 237.6157708 86.52850365 122.0530218 200.52908 67.05857911 167.910492 159.2613304 128.5719449 109.6605938 114.2178691 119.1551223 0 0 141.1120515 149.1150448 60.57401017 51.58670054 26.90397203 82.02401511 27.0841329 71.83180438 0 0 0 0 0 69.18267695 91.39296952 115.3368838 87.1463464 106.538154 0 167.2594201 215.3976222 70.03007684 87.49789192 152.1473424 87.2766444 173.0493218 148.8829811 110.894919 74.89399481 100.4927 73.3039023 104.6392403 0 36.23443253 41.54582022 14.11038175 15.26378314 15.96487334 34.85329086 156.2899081 153.2029901 0 0 112.3554796 0 85.47475943 67.08619701 43.00796287 0 0 0 0 0 0 0 135.9643412 136.2642373 291.9771812 70.89186607 100.9186965 85.95591723 41.24245498 44.3622579 75.42031834 54.44615294 22.50449201 25.55932373 67.045389 59.74956952 90.22463157 60.92187787 111.8238264 113.6660841 116.3288197 90.18188629 100.5627859 68.42374564 238.0875018 0 0 30.10079516 0 0 85.02017528 135.6205343 109.1934591 249.5522221 192.9561111 63.42468063 54.34329121 177.3752998 82.2728 63.37892611 0 60.36361631 153.8780862 67.83711271 80.18431213 HDCA 0 0 0 203.0489702 0 536.8728395 0 2060.108031 2244.224116 603.3784015 0 0 0 0 0 1178.587707 0 6681.076452 0 0 0 144.2909636 0 0 0 0 0 0 0 0 0 0 0 0 0 94.21734392 363.4650339 0 0 0 0 0 0 0 0 7550.606336 210.0490087 125.1038135 2384.835965 689.9241825 0 0 0 0 0 578.7055739 0 0 0 0 0 0 0 0 0 0 0 0 0 84.84122285 0 0 0 0 0 0 0 0 196.8101517 187.0168804 0 449.8078349 0 0 0 1355.188529 1491.062646 3761.349605 1721.298643 4047.000094 2034.833869 1449.734173 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 16.05838725 62.44142665 0 23.37494541 83.29686785 0 0 0 0 0 0 0 0 0 0 96.22689996 0 0 0 0 DHCA 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 33.11149656 47.65903234 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12-KCDCA 0 0 0 251.0118107 0 171.1491562 315.3567673 378.2125046 257.9475465 182.6500236 0 52.80454799 0 0 25.92819876 426.6875343 0 1216.401462 0 0 0 41.6350582 0 0 0 0 0 0 0 0 0 0 0 0 0 15.29905076 116.0657659 0 36.12540672 0 17.06266793 0 0 0 0 0 0 0 0 0 0 0 0 0 0 225.8333808 151.5693549 135.7869569 73.43304894 152.6695386 0 0 0 0 0 42.38142964 0 0 0 38.70957493 0 0 0 0 0 160.5876513 0 0 0 0 0 128.6870234 0 0.368900465 0 772.8875202 0 0 0 0 475.7823335 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7-KDCA 0 0 0 324.8450794 19.96037711 232.9530875 728.3922219 631.1470712 570.1638077 283.3190263 7.337270441 65.89669823 14.45003116 14.37939856 31.93191915 729.4422529 0 3458.749747 27.22026403 74.61253242 5.378533892 81.18678176 1.836265464 9.472053732 25.56679153 23.58568651 143.9668539 32.89750188 9.49676716 37.13339912 7.89528954 5.232959559 8.354929883 10.92597189 4.383106051 22.83339544 214.7190326 18.26261651 36.72936297 19.07487754 12.58284827 1.674248804 8.456901162 2.49800907 3.559094779 8140.852658 55.9239209 118.9820433 1220.281486 471.6680284 5.618718113 10.45648897 6.827267072 7.307831716 3.863812665 343.0066419 40.41849464 36.20985517 0 40.71187695 6.92650026 7.576269855 8.510079993 9.073714685 23.10892369 11.30171457 10.42893846 13.34269727 99.18435386 21.40512759 2.679905481 4.061696855 4.788439403 6.547979564 6.886993699 95.05850777 7.201740452 71.17698534 656.2420169 55.47105228 41.7287136 81.87723518 12.44807053 2.26200243 4.312828561 534.2901583 369.1708155 1614.974037 257.3519113 753.9174824 715.687263 128.3414028 10.50337951 13.06889517 8.441760128 21.25659171 26.6558761 7.964358399 14.39268288 10.47072756 13.39677759 6.231176217 4.393863479 6.483902022 11.09950198 10.59036329 8.411567204 4.29890784 12.82707532 12.43411048 9.154626829 6.104451517 10.16121042 20.48631088 24.88316741 89.86070799 254.5840332 29.74655013 55.80167521 174.6193024 29.07025652 11.81016255 8.901282718 27.76750967 19.13186799 39.62317122 14.6163637 37.92527972 23.83965026 3.466216032 80.63934718 36.09110562 50.0285852 11.87042742 8.135647934 UCA 12.35372329 9.37077925 11.09999897 79.8240263 15.85314553 58.65945131 57.61757203 119.6106088 348.7151961 45.38778876 10.22481047 13.81876114 12.6136551 8.108860995 15.29210828 468.9161566 10.63885917 5619.907999 33.07684014 60.01573455 11.16196441 21.42209756 8.74504403 16.38617366 62.05687975 7.76174505 16.24350536 15.96041715 8.21959916 20.13367033 9.047516613 10.12876842 7.15446498 12.22485033 8.764484281 13.0165144 35.64061228 10.58178409 17.77191414 8.087423752 16.51738685 5.253455 12.53581182 5.170729384 6.830513826 5955.743128 73.41052201 35.71315645 1575.790507 142.8165236 8.432164248 7.932858399 8.235824189 9.055751525 9.764738127 174.062272 20.20958513 23.43872883 11.91264505 9.807201131 10.01369005 5.795091365 9.303315516 8.792317981 14.8944577 7.174661891 13.61517515 8.136253355 44.67816103 21.67034329 4.53209818 5.623574817 5.007131338 3.910904594 4.363023003 24.72720656 8.814886579 18.05121701 255.1159088 30.81810793 17.83547503 55.8567305 8.617524164 4.426984036 3.848707962 864.135497 690.9980375 1535.915112 524.6488414 2070.053541 1299.6934 460.3243719 13.96972083 12.47303022 19.91205104 10.44753419 13.47502563 10.60662011 5.601825287 11.67887394 12.5565462 7.340632068 5.254605339 3.751872748 13.99633078 7.101551242 6.321033355 6.830296524 7.629432188 7.09346392 17.0401004 9.635683593 20.97618893 80.08433854 37.10581031 8.284355579 17.79801542 7.613764669 9.86319718 26.17233901 10.66079241 9.560943161 9.990464254 17.64736186 11.70972167 8.644869763 4.426435848 9.411707644 6.509459823 7.223425245 25.49225886 10.10257952 14.02830358 6.671367095 7.644070114 GHDCA 0.468059199 0.688145769 0.614519075 2.304021667 3.857983853 7.539765796 2.159100321 137.5883042 5.180271358 15.10135232 0.664053077 1.177962429 1.113167974 0.7370733 4.243174482 7.043333036 1.318231465 24.99616071 1.602876121 6.100218521 2.053935356 6.780603278 1.525136184 3.144449707 4.839828459 0.528627033 0.475118572 0.782158875 0.196369159 0.656122972 0.848279252 0.297091701 0.391471027 0.796420923 0.40566483 4.404284634 5.012559828 1.981864868 5.859765848 1.003332842 1.779898145 0.561907123 3.574487123 0.950637588 2.341629675 17.77978601 6.786658108 40.54501313 8.541235518 25.57111235 0.341491378 0.806813821 0.880634992 0.466740039 1.414189784 6.360473562 0.846914707 0.601985992 0.772553892 1.077228954 1.798710631 1.205699807 1.35975566 1.299943489 2.163627907 4.482471347 5.444769491 4.116215534 12.72235067 2.415767138 0.17334025 0.168063038 0.073891328 0.406707712 0.168952534 1.67300678 0.547192675 1.119326208 0.546337515 13.06034634 3.653294146 3.732026312 2.416489951 0.72796098 0.360781752 14.86890109 8.568169032 49.23620846 16.68778734 3.88878275 16.3642741 6.644340179 1.561096168 2.430873359 2.985223884 2.661358536 1.593616232 0.969676592 1.1559064 0.978132065 4.147969817 0.707277566 0.567921542 0 1.456053829 1.884089949 2.081163867 1.591498658 1.727470655 1.744342238 2.775957913 1.210426886 1.677378065 1.347436616 8.661191049 1.143990686 2.246674498 4.012430291 5.289035057 7.184007158 4.2419917 1.626840566 2.187422256 9.527831485 1.449205204 10.93458336 2.064292345 4.252049662 1.855254737 0.91769893 3.677305487 3.939680187 2.661899532 1.568708102 1.662336184 GUDCA 0.468059199 0.688145769 0.614519075 2.304021667 3.857983853 7.539765796 2.159100321 137.5883042 5.180271358 15.10135232 0.664053077 1.177962429 1.113167974 0.7370733 4.243174482 7.043333036 1.318231465 24.99616071 1.602876121 6.100218521 2.053935356 6.780603278 1.525136184 3.144449707 4.839828459 0.528627033 0.475118572 0.782158875 0.196369159 0.656122972 0.848279252 0.297091701 0.391471027 0.796420923 0.40566483 4.404284634 5.012559828 1.981864868 5.859765848 1.003332842 1.779898145 0.561907123 3.574487123 0.950637588 2.341629675 17.77978601 6.786658108 40.54501313 8.541235518 25.57111235 0.341491378 0.806813821 0.880634992 0.466740039 1.414189784 6.360473562 0.846914707 0.601985992 0.772553892 1.077228954 1.798710631 1.205699807 1.35975566 1.299943489 2.163627907 4.482471347 5.444769491 4.116215534 12.72235067 2.415767138 0.17334025 0 0 0.406707712 0.168952534 1.67300678 0.547192675 1.119326208 0.546337515 13.06034634 3.653294146 3.732026312 2.416489951 0.72796098 0.360781752 14.86890109 8.568169032 49.23620846 16.68778734 3.88878275 16.3642741 6.644340179 1.561096168 2.430873359 2.985223884 2.661358536 1.593616232 0.969676592 1.1559064 0.978132065 4.147969817 0.707277566 0.567921542 0 1.456053829 1.884089949 2.081163867 1.591498658 1.727470655 1.744342238 2.775957913 1.210426886 1.677378065 1.347436616 8.661191049 1.143990686 2.246674498 4.012430291 5.289035057 7.184007158 4.2419917 1.626840566 2.187422256 9.527831485 1.449205204 10.93458336 2.064292345 4.252049662 1.855254737 0.91769893 3.677305487 3.939680187 2.661899532 1.568708102 1.662336184 THCA 11.46961861 8.32365161 28.55858791 61.30345682 66.71989294 159.1707109 107.2004701 236.0958074 229.4833145 236.7738859 12.6244786 69.38576028 23.77965534 10.41737407 19.73336935 515.9178878 56.76028642 1695.134306 469.6469709 469.6605585 188.6740537 102.2783165 118.6082311 169.0805004 230.8954972 146.1353953 251.7023219 404.2479336 95.7518099 478.9186097 30.72341605 1.793829105 3.415491434 17.31048074 17.14301039 236.3046404 266.9954627 85.00078403 233.9689842 227.1163548 14.04984625 23.10016372 20.62552553 14.64335949 29.86235078 768.4318523 52.58986678 90.94222009 240.3679245 245.5010987 4.690435904 3.997889999 2.741146475 3.048611683 5.841017285 170.8539 45.79648052 76.35572138 55.69543456 70.15843953 67.10749956 20.72091694 44.0238907 59.35557195 24.05683491 92.81380751 134.0521056 40.93547903 51.03287536 29.46111894 40.85930901 59.33245196 10.76942161 12.13182527 10.94535018 26.85119462 84.33688524 121.8184836 162.8549077 64.08444826 88.89727536 85.00551874 24.47178684 29.76302158 20.23217487 124.0235766 165.7310423 347.0317513 355.6042405 314.9885363 86.28808182 173.5441556 38.87616439 29.42563435 48.63000655 28.94717105 47.5379144 46.35817841 21.36832699 32.0904667 23.54536019 14.67056761 7.72012717 5.557794607 22.74579974 4.528280845 12.11526921 5.354319307 11.92593308 11.27642638 61.67789887 27.80161527 76.3710523 95.83886751 87.31193225 8.545677674 22.76858895 11.61841894 9.426544542 32.34132078 47.08709877 17.01226946 19.1121235 62.10989061 33.34341854 28.67959217 24.85311032 38.40154119 22.26805273 18.11831067 83.56403455 46.24342559 98.87651091 82.60392898 53.99366264 TUDCA 0 0 0 2.253063327 1.412542703 6.159796899 0.820200881 119.5182502 5.219875723 10.33456935 0 9.297504836 1.125391763 0 1.711911685 3.884059331 0.681405055 29.89380083 1.468237665 2.533245395 3.870438309 1.860110443 0.525925409 3.57883075 0 0 0 0.946212328 0 0 0 0 0 0 0 1.144110723 1.729236075 1.253887735 1.638469599 0.865719146 1.036519171 0.664949569 2.471346841 0 1.513289801 34.09595735 2.93110012 15.72458789 4.541440564 12.62055477 0 0 0 0 0.729320723 1.408481955 1.007502584 0 0 0.608358851 0.795598179 0.641229247 0.759687831 0.778698255 1.163592651 2.9940739 1.987145204 2.034686606 10.05982528 1.609120122 0 0 0 0 0 4.233748228 5.926107746 2.299643553 1.064302768 13.2105104 5.826474646 9.778279804 2.042940866 0 0 125.9622312 12.47957486 67.87816642 7.389351664 7.199703776 24.01067768 25.27210847 2.208509542 4.534298259 4.586662553 2.081357133 1.456145929 0.809593271 0 1.139229651 1.27263771 0 0 0 0 0 0 0 1.891920237 1.201871459 0.529903535 0 0.734107438 0.772057235 2.458183346 0.633338719 1.363257696 2.04917032 3.624310805 5.642213107 1.052334107 0 0 2.001004767 0.964108906 1.89952752 0 0.712639692 0 0 3.723862649 2.109898797 2.188922834 1.030385417 0 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name full_name m/z RT PubChem ID GCA glycocholic acid 464.3/74.0 5.07 10140 GCDCA glycochenodeoxycholic acid 448.3/74.0 7.48 12544 CA cholic acid 407.3/407.3 7.09 221493 CDCA chenodeoxycholic acid 391.3/391.3 10.08 10133 LCA lithocholic acid 375.3/375.3 12.62 9903 DCA deoxycholic acid 391.3/391.3 10.46 222528 GDCA glycodeoxycholic acid 448.3/74.0 7.97 3035026 GLCA glycolithocholic acid 432.3/74.0 10.86 115245 TCA taurocholic acid 514.3/80.0 5.33 6675 TCDCA taurochenodeoxycholic aicd 498.3/80.0 7.94 387316 TLCA taurolithocholic acid 482.3/80.0 11.39 439763 TDCA taurodeoxycholic acid 498.3/80.0 8.56 2733768 DHLCA dehydrolithocholic acid 373.3/373.3 12.56 5283906 NDCA nordeoxycholic acid 377.3/377.3 8.89 193905 7-KCDCA 7-ketochenodeoxycholic acid 389.3/389.3 8.49 UDCA ursodeoxycholic acid 391.3/391.3 7.5 31401 HDCA hyodeoxycholic acid 391.3/391.3 7.49 5283820 DHCA dehydrocholic acid 391.3/391.3 6.83 6674 12-KCDCA 12-ketochenodeoxycholic acid 405.3/405.3 5.42 94235 7-KDCA 7-ketodeoxycholic acid 405.3/405.3 5.42 188292 UCA ursocholic acid 407.3/407.3 4.04 122340 GHDCA glycohyodeoxycholic acid 448.3/74.0 5.06 114611 GUDCA glycoursodeoxycholic acid 448.3/74.0 5.06 12310288 THCA coprocholic acid/trihydroxycholestanoic acid 449.3/449.3 11.03 122312 TUDCA tauroursodeoxycholic acid 498.3/80.0 5.53 9848818 METABOLITES_END #END