#METABOLOMICS WORKBENCH EllenDePaepe_20230706_002000 DATATRACK_ID:4143 STUDY_ID:ST002944 ANALYSIS_ID:AN004829 PROJECT_ID:PR001831
VERSION             	1
CREATED_ON             	October 27, 2023, 10:00 am
#PROJECT
PR:PROJECT_TITLE                 	Integrated gut metabolome and microbiome fingerprinting reveals that dysbiosis
PR:PROJECT_TITLE                 	precedes allergic inflammation in IgE-mediated pediatric cow’s milk allergy
PR:PROJECT_TYPE                  	(un)targeted MS
PR:PROJECT_SUMMARY               	Background: IgE-mediated cow’s milk allergy (IgE-CMA) is one of the first
PR:PROJECT_SUMMARY               	allergies to arise in early childhood and may result from exposure to various
PR:PROJECT_SUMMARY               	milk allergens, of which β-lactoglobulin (BLG) and casein are the most
PR:PROJECT_SUMMARY               	important. Understanding the underlying mechanisms behind IgE-CMA is imperative
PR:PROJECT_SUMMARY               	for the discovery of novel biomarkers and the design of innovative treatment and
PR:PROJECT_SUMMARY               	prevention strategies. Methods: We report a longitudinal in vivo murine model,
PR:PROJECT_SUMMARY               	in which 2 mice strains (BALB/c and C57Bl/6) were sensitized to BLG using either
PR:PROJECT_SUMMARY               	cholera toxin or an oil emulsion (n=6 per group). After sensitization, mice were
PR:PROJECT_SUMMARY               	challenged orally, their clinical signs monitored, antibody (IgE and IgG1) and
PR:PROJECT_SUMMARY               	cytokine levels (IL-4 and IFN-γ) measured, and fecal samples subjected to
PR:PROJECT_SUMMARY               	metabolomics. The results of the murine models were further supported by fecal
PR:PROJECT_SUMMARY               	microbiome-metabolome data from our population of IgE-CMA (n=24) and healthy
PR:PROJECT_SUMMARY               	(n=23) children (Trial: NCT04249973), on which polar metabolomics, lipidomics
PR:PROJECT_SUMMARY               	and 16S rRNA metasequencing were performed. In vitro gastrointestinal digestions
PR:PROJECT_SUMMARY               	and multi-omics corroborated the microbial origin of proposed metabolic changes.
PR:PROJECT_SUMMARY               	Results: During sensitization, we observed multiple microbially derived
PR:PROJECT_SUMMARY               	metabolic alterations, most importantly bile acid, energy and tryptophan
PR:PROJECT_SUMMARY               	metabolites, that preceded allergic inflammation. The latter was reflected in a
PR:PROJECT_SUMMARY               	disturbed sphingolipid metabolism. We confirmed microbial dysbiosis, and its
PR:PROJECT_SUMMARY               	causal effect on metabolic alterations in our patient cohort, which was
PR:PROJECT_SUMMARY               	accompanied by metabolic signatures of low-grade inflammation. Conclusion: Our
PR:PROJECT_SUMMARY               	results indicate that gut dysbiosis precedes allergic inflammation and nurtures
PR:PROJECT_SUMMARY               	a chronic low-grade inflammation in children on elimination diets, opening
PR:PROJECT_SUMMARY               	important new opportunities for future prevention and treatment strategies.
PR:INSTITUTE                     	Ghent University
PR:DEPARTMENT                    	Translational Physiology, Infectiology and Public Health
PR:LABORATORY                    	Laboratory for Integrative Metabolomics
PR:LAST_NAME                     	De Paepe
PR:FIRST_NAME                    	Ellen
PR:ADDRESS                       	Salisburylaan 133, Merelbeke, Oost-Vlaanderen, 9820, Belgium
PR:EMAIL                         	Ellen.DePaepe@UGent.be
PR:PHONE                         	0032479081098
PR:FUNDING_SOURCE                	Fonds Wetenschappelijk Onderzoek
#STUDY
ST:STUDY_TITLE                   	Longitudinal polar fecal metabolomics of mice undergoing sensitization to
ST:STUDY_TITLE                   	beta-lactoglobulin
ST:STUDY_SUMMARY                 	This study is part of a multi-part study, including a. longitudinal polar fecal
ST:STUDY_SUMMARY                 	metabolomics of mice undergoing sensitization to beta-lactoglobulin b. polar
ST:STUDY_SUMMARY                 	fecal metabolomics of a patient cohort c. fecal lipidomics of a patient cohort
ST:STUDY_SUMMARY                 	d. polar urinary metabolomics of a patient cohort e. polar metabolomics of in
ST:STUDY_SUMMARY                 	vitro digestions This specific part is part a. longitudinal polar fecal
ST:STUDY_SUMMARY                 	metabolomics of mice undergoing sensitization to beta-lactoglobulin
ST:INSTITUTE                     	Ghent University
ST:DEPARTMENT                    	Department of Translational Physiology, Infectiology and Public Health
ST:LABORATORY                    	Laboratory for Integrative Metabolomics
ST:LAST_NAME                     	De Paepe
ST:FIRST_NAME                    	Ellen
ST:ADDRESS                       	Salisburylaan 133, 9820 Merelbeke, Belgium
ST:EMAIL                         	Ellen.DePaepe@UGent.be
ST:STUDY_TYPE                    	(un)targeted MS
ST:PHONE                         	0032479081098
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENOTYPE_STRAIN               	BALB/c and C57Bl/6
SU:AGE_OR_AGE_RANGE              	3-10 weeks
SU:GENDER                        	Female
SU:ANIMAL_ANIMAL_SUPPLIER        	Charles River
SU:ANIMAL_HOUSING                	SPF
SU:ANIMAL_FEED                   	Sniff
SU:ANIMAL_WATER                  	Ad libitum
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_3	Strain:BC | treatment:5W | week:Wk 3	RAW_FILE_NAME=210611_se021.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_4	Strain:BC | treatment:6Z | week:Wk 7	RAW_FILE_NAME=210611_se022.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_5	Strain:B6 | treatment:4W | week:Wk 1	RAW_FILE_NAME=210611_se023.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_7	Strain:BC | treatment:4Z | week:Wk 5	RAW_FILE_NAME=210611_se024.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_8	Strain:B6 | treatment:2D | week:Wk 4	RAW_FILE_NAME=210611_se025.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_9	Strain:BC | treatment:5X | week:Wk 7	RAW_FILE_NAME=210611_se026.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_10	Strain:BC | treatment:1D | week:Wk 1	RAW_FILE_NAME=210611_se027.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_11	Strain:BC | treatment:5F | week:Wk 2	RAW_FILE_NAME=210611_se028.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_12	Strain:B6 | treatment:4X | week:Wk 7	RAW_FILE_NAME=210611_se029.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_13	Strain:B6 | treatment:4Y | week:Wk 7	RAW_FILE_NAME=210611_se030.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC1	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se031.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC2	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se032.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_14	Strain:BC | treatment:4F | week:Wk 2	RAW_FILE_NAME=210611_se034.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_15	Strain:BC | treatment:2C | week:Wk 1	RAW_FILE_NAME=210611_se035.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_16	Strain:B6 | treatment:1W | week:Wk 1	RAW_FILE_NAME=210611_se036.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_17	Strain:BC | treatment:1Y | week:Wk 1	RAW_FILE_NAME=210611_se037.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_18	Strain:B6 | treatment:6C | week:Wk 1	RAW_FILE_NAME=210611_se038.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_19	Strain:BC | treatment:6Z | week:Wk 5	RAW_FILE_NAME=210611_se039.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_20	Strain:B6 | treatment:5D | week:Wk 4	RAW_FILE_NAME=210611_se040.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_21	Strain:B6 | treatment:3D | week:Wk 4	RAW_FILE_NAME=210611_se041.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_22	Strain:BC | treatment:2B | week:Wk 1	RAW_FILE_NAME=210611_se042.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_23	Strain:B6 | treatment:2F | week:Wk 4	RAW_FILE_NAME=210611_se043.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC3	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se044.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC4	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se045.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_24	Strain:BC | treatment:5F | week:Wk 1	RAW_FILE_NAME=210611_se047.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_26	Strain:BC | treatment:6C | week:Wk 1	RAW_FILE_NAME=210611_se048.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_27	Strain:BC | treatment:6B | week:Wk 4	RAW_FILE_NAME=210611_se049.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_28	Strain:B6 | treatment:6D | week:Wk 4	RAW_FILE_NAME=210611_se050.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_29	Strain:BC | treatment:1Z | week:Wk 7	RAW_FILE_NAME=210611_se051.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_31	Strain:B6 | treatment:1F | week:Wk 1	RAW_FILE_NAME=210611_se052.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_32	Strain:B6 | treatment:6X | week:Wk 1	RAW_FILE_NAME=210611_se053.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_34	Strain:B6 | treatment:1E | week:Wk 4	RAW_FILE_NAME=210611_se054.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_35	Strain:B6 | treatment:1A | week:Wk 1	RAW_FILE_NAME=210611_se055.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_36	Strain:B6 | treatment:5X | week:Wk 1	RAW_FILE_NAME=210611_se056.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC5	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se057.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC6	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se058.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_37	Strain:B6 | treatment:5Y | week:Wk 1	RAW_FILE_NAME=210611_se060.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_38	Strain:BC | treatment:1F | week:Wk 1	RAW_FILE_NAME=210611_se061.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_39	Strain:BC | treatment:2W | week:Wk 3	RAW_FILE_NAME=210611_se062.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_41	Strain:B6 | treatment:3Z | week:Wk 7	RAW_FILE_NAME=210611_se063.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_43	Strain:BC | treatment:6W | week:Wk 5	RAW_FILE_NAME=210611_se064.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_44	Strain:BC | treatment:1E | week:Wk 1	RAW_FILE_NAME=210611_se065.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_45	Strain:BC | treatment:1X | week:Wk 1	RAW_FILE_NAME=210611_se066.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_46	Strain:BC | treatment:2A | week:Wk 4	RAW_FILE_NAME=210611_se067.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_47	Strain:B6 | treatment:4E | week:Wk 4	RAW_FILE_NAME=210611_se068.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_48	Strain:BC | treatment:3E | week:Wk 4	RAW_FILE_NAME=210611_se069.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC7	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se070.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC8	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se071.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_49	Strain:BC | treatment:2E | week:Wk 4	RAW_FILE_NAME=210611_se073.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_50	Strain:B6 | treatment:5F | week:Wk 1	RAW_FILE_NAME=210611_se074.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_51	Strain:B6 | treatment:6F | week:Wk 1	RAW_FILE_NAME=210611_se075.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_52	Strain:BC | treatment:4Z | week:Wk 3	RAW_FILE_NAME=210611_se076.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_53	Strain:B6 | treatment:6A | week:Wk 4	RAW_FILE_NAME=210611_se077.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_54	Strain:B6 | treatment:6W | week:Wk 1	RAW_FILE_NAME=210611_se078.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_55	Strain:B6 | treatment:5D | week:Wk 1	RAW_FILE_NAME=210611_se079.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_56	Strain:B6 | treatment:4B | week:Wk 1	RAW_FILE_NAME=210611_se080.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_57	Strain:B6 | treatment:6E | week:Wk 4	RAW_FILE_NAME=210611_se081.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_58	Strain:B6 | treatment:5E | week:Wk 1	RAW_FILE_NAME=210611_se082.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC9	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se083.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC10	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se084.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_59	Strain:BC | treatment:2E | week:Wk 2	RAW_FILE_NAME=210611_se086.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_60	Strain:B6 | treatment:3C | week:Wk 1	RAW_FILE_NAME=210611_se087.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_61	Strain:B6 | treatment:6A | week:Wk 1	RAW_FILE_NAME=210611_se088.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_62	Strain:B6 | treatment:5B | week:Wk 1	RAW_FILE_NAME=210611_se089.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_63	Strain:BC | treatment:1B | week:Wk 4	RAW_FILE_NAME=210611_se090.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_64	Strain:BC | treatment:4Y | week:Wk 7	RAW_FILE_NAME=210611_se091.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_65	Strain:BC | treatment:4D | week:Wk 4	RAW_FILE_NAME=210611_se092.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_66	Strain:BC | treatment:2F | week:Wk 4	RAW_FILE_NAME=210611_se093.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_67	Strain:BC | treatment:3Z | week:Wk 3	RAW_FILE_NAME=210611_se094.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_68	Strain:B6 | treatment:6X | week:Wk 7	RAW_FILE_NAME=210611_se095.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC11	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se096.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC12	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se097.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_69	Strain:BC | treatment:1Z | week:Wk 3	RAW_FILE_NAME=210611_se101.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_70	Strain:BC | treatment:5F | week:Wk 3	RAW_FILE_NAME=210611_se102.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_71	Strain:B6 | treatment:4B | week:Wk 4	RAW_FILE_NAME=210611_se103.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_72	Strain:BC | treatment:1E | week:Wk 4	RAW_FILE_NAME=210611_se104.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_73	Strain:B6 | treatment:3B | week:Wk 4	RAW_FILE_NAME=210611_se105.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_74	Strain:BC | treatment:3Z | week:Wk 7	RAW_FILE_NAME=210611_se106.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_75	Strain:B6 | treatment:1D | week:Wk 4	RAW_FILE_NAME=210611_se107.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_76	Strain:BC | treatment:6A | week:Wk 3	RAW_FILE_NAME=210611_se108.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_77	Strain:BC | treatment:4E | week:Wk 2	RAW_FILE_NAME=210611_se109.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_78	Strain:BC | treatment:4X | week:Wk 7	RAW_FILE_NAME=210611_se110.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC13	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se111.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC14	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se112.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_79	Strain:BC | treatment:5A | week:Wk 3	RAW_FILE_NAME=210611_se114.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_80	Strain:B6 | treatment:6B | week:Wk 4	RAW_FILE_NAME=210611_se115.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_81	Strain:BC | treatment:4W | week:Wk 7	RAW_FILE_NAME=210611_se116.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_82	Strain:BC | treatment:6F | week:Wk 2	RAW_FILE_NAME=210611_se117.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_83	Strain:BC | treatment:4Z | week:Wk 7	RAW_FILE_NAME=210611_se118.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_84	Strain:BC | treatment:2B | week:Wk 2	RAW_FILE_NAME=210611_se119.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_85	Strain:BC | treatment:3B | week:Wk 3	RAW_FILE_NAME=210611_se120.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_86	Strain:B6 | treatment:2E | week:Wk 1	RAW_FILE_NAME=210611_se121.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_87	Strain:BC | treatment:2B | week:Wk 4	RAW_FILE_NAME=210611_se122.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_88	Strain:BC | treatment:2D | week:Wk 4	RAW_FILE_NAME=210611_se123.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC15	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se124.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC16	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se125.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_90	Strain:BC | treatment:2Z | week:Wk 5	RAW_FILE_NAME=210611_se127.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_92	Strain:BC | treatment:5E | week:Wk 2	RAW_FILE_NAME=210611_se128.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_93	Strain:B6 | treatment:2A | week:Wk 4	RAW_FILE_NAME=210611_se129.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_94	Strain:BC | treatment:5B | week:Wk 2	RAW_FILE_NAME=210611_se130.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_95	Strain:BC | treatment:3E | week:Wk 3	RAW_FILE_NAME=210611_se131.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_96	Strain:BC | treatment:4B | week:Wk 4	RAW_FILE_NAME=210611_se132.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_97	Strain:BC | treatment:3A | week:Wk 1	RAW_FILE_NAME=210611_se133.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_98	Strain:BC | treatment:3F | week:Wk 3	RAW_FILE_NAME=210611_se134.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_101	Strain:B6 | treatment:5C | week:Wk 4	RAW_FILE_NAME=210611_se135.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_102	Strain:B6 | treatment:5C | week:Wk 1	RAW_FILE_NAME=210611_se136.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC17	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se137.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC18	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se138.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_103	Strain:B6 | treatment:4C | week:Wk 4	RAW_FILE_NAME=210611_se140.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_104	Strain:BC | treatment:1W | week:Wk 1	RAW_FILE_NAME=210611_se141.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_105	Strain:B6 | treatment:6Y | week:Wk 7	RAW_FILE_NAME=210611_se142.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_106	Strain:BC | treatment:4C | week:Wk 4	RAW_FILE_NAME=210611_se143.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_107	Strain:BC | treatment:5W | week:Wk 7	RAW_FILE_NAME=210611_se144.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_108	Strain:BC | treatment:5C | week:Wk 1	RAW_FILE_NAME=210611_se145.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_110	Strain:BC | treatment:1F | week:Wk 3	RAW_FILE_NAME=210611_se146.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_111	Strain:BC | treatment:5Z | week:Wk 1	RAW_FILE_NAME=210611_se147.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_112	Strain:BC | treatment:6F | week:Wk 4	RAW_FILE_NAME=210611_se148.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_113	Strain:BC | treatment:2Y | week:Wk 7	RAW_FILE_NAME=210611_se149.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC19	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se150.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC20	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se151.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_114	Strain:BC | treatment:1E | week:Wk 3	RAW_FILE_NAME=210611_se153.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_116	Strain:B6 | treatment:3W | week:Wk 1	RAW_FILE_NAME=210611_se154.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_117	Strain:BC | treatment:3B | week:Wk 1	RAW_FILE_NAME=210611_se155.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_118	Strain:B6 | treatment:3Z | week:Wk 1	RAW_FILE_NAME=210611_se156.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_120	Strain:BC | treatment:4F | week:Wk 4	RAW_FILE_NAME=210611_se157.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_121	Strain:BC | treatment:5E | week:Wk 4	RAW_FILE_NAME=210611_se158.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_122	Strain:BC | treatment:5B | week:Wk 1	RAW_FILE_NAME=210611_se159.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_123	Strain:BC | treatment:4A | week:Wk 4	RAW_FILE_NAME=210611_se160.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_124	Strain:B6 | treatment:1F | week:Wk 4	RAW_FILE_NAME=210611_se161.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_125	Strain:B6 | treatment:1E | week:Wk 1	RAW_FILE_NAME=210611_se162.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC21	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se163.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC22	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se164.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_126	Strain:B6 | treatment:2W | week:Wk 1	RAW_FILE_NAME=210611_se166.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_128	Strain:B6 | treatment:6D | week:Wk 1	RAW_FILE_NAME=210611_se167.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_129	Strain:B6 | treatment:1C | week:Wk 1	RAW_FILE_NAME=210611_se168.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_130	Strain:BC | treatment:4W | week:Wk 3	RAW_FILE_NAME=210611_se169.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_131	Strain:BC | treatment:4E | week:Wk 3	RAW_FILE_NAME=210611_se170.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_132	Strain:B6 | treatment:3A | week:Wk 4	RAW_FILE_NAME=210611_se171.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_134	Strain:BC | treatment:3W | week:Wk 3	RAW_FILE_NAME=210611_se172.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_136	Strain:BC | treatment:6E | week:Wk 2	RAW_FILE_NAME=210611_se173.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_137	Strain:BC | treatment:5Z | week:Wk 5	RAW_FILE_NAME=210611_se174.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_138	Strain:BC | treatment:3B | week:Wk 2	RAW_FILE_NAME=210611_se175.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC23	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se176.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC24	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se177.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_139	Strain:B6 | treatment:5Z | week:Wk 7	RAW_FILE_NAME=210611_se179.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_140	Strain:BC | treatment:2E | week:Wk 1	RAW_FILE_NAME=210611_se180.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_141	Strain:BC | treatment:4E | week:Wk 1	RAW_FILE_NAME=210611_se181.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_142	Strain:BC | treatment:5B | week:Wk 4	RAW_FILE_NAME=210611_se182.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_143	Strain:BC | treatment:3A | week:Wk 3	RAW_FILE_NAME=210611_se183.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_144	Strain:BC | treatment:2Z | week:Wk 7	RAW_FILE_NAME=210611_se184.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_145	Strain:BC | treatment:2A | week:Wk 2	RAW_FILE_NAME=210611_se185.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_146	Strain:BC | treatment:3X | week:Wk 7	RAW_FILE_NAME=210611_se186.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_147	Strain:BC | treatment:6E | week:Wk 1	RAW_FILE_NAME=210611_se187.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_149	Strain:BC | treatment:6Z | week:Wk 1	RAW_FILE_NAME=210611_se188.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC25	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se189.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC26	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se190.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_150	Strain:BC | treatment:5Y | week:Wk 1	RAW_FILE_NAME=210611_se192.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_151	Strain:B6 | treatment:4A | week:Wk 4	RAW_FILE_NAME=210611_se193.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_152	Strain:BC | treatment:4A | week:Wk 3	RAW_FILE_NAME=210611_se194.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_153	Strain:BC | treatment:4A | week:Wk 2	RAW_FILE_NAME=210611_se195.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_155	Strain:BC | treatment:3C | week:Wk 1	RAW_FILE_NAME=210611_se196.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_156	Strain:BC | treatment:5Z | week:Wk 7	RAW_FILE_NAME=210611_se197.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_157	Strain:BC | treatment:6F | week:Wk 3	RAW_FILE_NAME=210611_se198.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_158	Strain:B6 | treatment:2E | week:Wk 4	RAW_FILE_NAME=210611_se199.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_159	Strain:BC | treatment:2A | week:Wk 3	RAW_FILE_NAME=210611_se200.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_160	Strain:BC | treatment:3Z | week:Wk 5	RAW_FILE_NAME=210611_se201.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC27	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se202.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC28	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se203.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_161	Strain:BC | treatment:4B | week:Wk 2	RAW_FILE_NAME=210611_se207.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_162	Strain:BC | treatment:6B | week:Wk 2	RAW_FILE_NAME=210611_se208.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_163	Strain:BC | treatment:6W | week:Wk 7	RAW_FILE_NAME=210611_se209.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_164	Strain:BC | treatment:2E | week:Wk 3	RAW_FILE_NAME=210611_se210.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_166	Strain:BC | treatment:1W | week:Wk 5	RAW_FILE_NAME=210611_se211.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_167	Strain:BC | treatment:1E | week:Wk 2	RAW_FILE_NAME=210611_se212.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_168	Strain:B6 | treatment:4Y | week:Wk 1	RAW_FILE_NAME=210611_se213.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_169	Strain:BC | treatment:3F | week:Wk 4	RAW_FILE_NAME=210611_se214.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_170	Strain:BC | treatment:6Z | week:Wk 3	RAW_FILE_NAME=210611_se215.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_171	Strain:BC | treatment:6E | week:Wk 4	RAW_FILE_NAME=210611_se216.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC29	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se217.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC30	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se218.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_172	Strain:B6 | treatment:4D | week:Wk 4	RAW_FILE_NAME=210611_se220.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_173	Strain:BC | treatment:6A | week:Wk 2	RAW_FILE_NAME=210611_se221.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_174	Strain:B6 | treatment:5X | week:Wk 7	RAW_FILE_NAME=210611_se222.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_175	Strain:BC | treatment:5C | week:Wk 4	RAW_FILE_NAME=210611_se223.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_176	Strain:BC | treatment:6W | week:Wk 3	RAW_FILE_NAME=210611_se224.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_177	Strain:BC | treatment:2W | week:Wk 1	RAW_FILE_NAME=210611_se225.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_178	Strain:BC | treatment:1Y | week:Wk 7	RAW_FILE_NAME=210611_se226.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_179	Strain:B6 | treatment:1B | week:Wk 4	RAW_FILE_NAME=210611_se227.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_180	Strain:BC | treatment:6C | week:Wk 4	RAW_FILE_NAME=210611_se228.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_181	Strain:BC | treatment:5A | week:Wk 1	RAW_FILE_NAME=210611_se229.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC31	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se230.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC32	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se231.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_184	Strain:B6 | treatment:5B | week:Wk 4	RAW_FILE_NAME=210611_se233.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_185	Strain:BC | treatment:2X | week:Wk 7	RAW_FILE_NAME=210611_se234.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_187	Strain:BC | treatment:1F | week:Wk 4	RAW_FILE_NAME=210611_se235.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_188	Strain:BC | treatment:6X | week:Wk 7	RAW_FILE_NAME=210611_se236.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_189	Strain:BC | treatment:5D | week:Wk 1	RAW_FILE_NAME=210611_se237.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_190	Strain:B6 | treatment:1X | week:Wk 1	RAW_FILE_NAME=210611_se238.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_191	Strain:B6 | treatment:1A | week:Wk 4	RAW_FILE_NAME=210611_se239.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_192	Strain:BC | treatment:3D | week:Wk 1	RAW_FILE_NAME=210611_se240.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_193	Strain:B6 | treatment:2C | week:Wk 4	RAW_FILE_NAME=210611_se241.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_194	Strain:BC | treatment:2Z | week:Wk 1	RAW_FILE_NAME=210611_se242.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC33	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se243.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC34	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se244.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_195	Strain:B6 | treatment:2B | week:Wk 4	RAW_FILE_NAME=210611_se246.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_197	Strain:BC | treatment:3C | week:Wk 4	RAW_FILE_NAME=210611_se247.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_198	Strain:B6 | treatment:4D | week:Wk 1	RAW_FILE_NAME=210611_se248.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_199	Strain:B6 | treatment:6W | week:Wk 7	RAW_FILE_NAME=210611_se249.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_200	Strain:BC | treatment:6D | week:Wk 1	RAW_FILE_NAME=210611_se250.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_201	Strain:BC | treatment:1B | week:Wk 3	RAW_FILE_NAME=210611_se251.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_202	Strain:B6 | treatment:4C | week:Wk 1	RAW_FILE_NAME=210611_se252.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_204	Strain:BC | treatment:1W | week:Wk 7	RAW_FILE_NAME=210611_se253.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_205	Strain:B6 | treatment:1C | week:Wk 4	RAW_FILE_NAME=210611_se254.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_206	Strain:B6 | treatment:5F | week:Wk 4	RAW_FILE_NAME=210611_se255.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC35	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se256.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC36	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se257.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_207	Strain:B6 | treatment:2Y | week:Wk 7	RAW_FILE_NAME=210611_se261.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_208	Strain:BC | treatment:1D | week:Wk 4	RAW_FILE_NAME=210611_se262.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_209	Strain:B6 | treatment:5E | week:Wk 4	RAW_FILE_NAME=210611_se263.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_210	Strain:BC | treatment:1A | week:Wk 3	RAW_FILE_NAME=210611_se264.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_211	Strain:B6 | treatment:2C | week:Wk 1	RAW_FILE_NAME=210611_se265.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_212	Strain:BC | treatment:3F | week:Wk 2	RAW_FILE_NAME=210611_se266.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_213	Strain:BC | treatment:6F | week:Wk 1	RAW_FILE_NAME=210611_se267.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_214	Strain:B6 | treatment:6F | week:Wk 4	RAW_FILE_NAME=210611_se268.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_215	Strain:B6 | treatment:6Y | week:Wk 1	RAW_FILE_NAME=210611_se269.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_216	Strain:BC | treatment:6B | week:Wk 3	RAW_FILE_NAME=210611_se270.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC37	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se271.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC38	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se272.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_217	Strain:BC | treatment:2F | week:Wk 2	RAW_FILE_NAME=210611_se274.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_218	Strain:BC | treatment:5F | week:Wk 4	RAW_FILE_NAME=210611_se275.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_219	Strain:BC | treatment:3Y | week:Wk 1	RAW_FILE_NAME=210611_se276.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_220	Strain:BC | treatment:1F | week:Wk 2	RAW_FILE_NAME=210611_se277.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_221	Strain:BC | treatment:3W | week:Wk 5	RAW_FILE_NAME=210611_se278.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_222	Strain:B6 | treatment:3C | week:Wk 4	RAW_FILE_NAME=210611_se279.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_223	Strain:BC | treatment:6Y | week:Wk 1	RAW_FILE_NAME=210611_se280.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_224	Strain:B6 | treatment:6Z | week:Wk 7	RAW_FILE_NAME=210611_se281.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_225	Strain:BC | treatment:6B | week:Wk 1	RAW_FILE_NAME=210611_se282.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_226	Strain:BC | treatment:1B | week:Wk 2	RAW_FILE_NAME=210611_se283.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC39	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se284.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC40	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se285.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_227	Strain:BC | treatment:1A | week:Wk 4	RAW_FILE_NAME=210611_se287.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_229	Strain:B6 | treatment:4F | week:Wk 4	RAW_FILE_NAME=210611_se288.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_230	Strain:BC | treatment:2Z | week:Wk 3	RAW_FILE_NAME=210611_se289.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_232	Strain:BC | treatment:6E | week:Wk 3	RAW_FILE_NAME=210611_se290.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_233	Strain:B6 | treatment:5A | week:Wk 4	RAW_FILE_NAME=210611_se291.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_235	Strain:BC | treatment:3D | week:Wk 4	RAW_FILE_NAME=210611_se292.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_236	Strain:B6 | treatment:1Z | week:Wk 7	RAW_FILE_NAME=210611_se293.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_237	Strain:BC | treatment:5E | week:Wk 3	RAW_FILE_NAME=210611_se294.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_238	Strain:BC | treatment:6D | week:Wk 4	RAW_FILE_NAME=210611_se295.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_239	Strain:BC | treatment:3Y | week:Wk 7	RAW_FILE_NAME=210611_se296.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC41	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se297.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC42	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se298.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_240	Strain:BC | treatment:2F | week:Wk 1	RAW_FILE_NAME=210611_se302.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_242	Strain:BC | treatment:4E | week:Wk 4	RAW_FILE_NAME=210611_se303.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_243	Strain:BC | treatment:4Z | week:Wk 1	RAW_FILE_NAME=210611_se304.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_244	Strain:B6 | treatment:2X | week:Wk 7	RAW_FILE_NAME=210611_se305.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_245	Strain:B6 | treatment:1Y | week:Wk 7	RAW_FILE_NAME=210611_se306.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_246	Strain:BC | treatment:2Y | week:Wk 1	RAW_FILE_NAME=210611_se307.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_251	Strain:BC | treatment:1B | week:Wk 1	RAW_FILE_NAME=210611_se308.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_252	Strain:B6 | treatment:5A | week:Wk 1	RAW_FILE_NAME=210611_se309.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_253	Strain:BC | treatment:5Z | week:Wk 3	RAW_FILE_NAME=210611_se310.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_254	Strain:B6 | treatment:3A | week:Wk 1	RAW_FILE_NAME=210611_se311.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC43	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se312.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC44	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se313.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_255	Strain:BC | treatment:5A | week:Wk 2	RAW_FILE_NAME=210611_se315.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_256	Strain:BC | treatment:2C | week:Wk 4	RAW_FILE_NAME=210611_se316.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_257	Strain:BC | treatment:1Z | week:Wk 1	RAW_FILE_NAME=210611_se317.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_258	Strain:BC | treatment:6Y | week:Wk 7	RAW_FILE_NAME=210611_se318.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_259	Strain:BC | treatment:4F | week:Wk 1	RAW_FILE_NAME=210611_se319.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_260	Strain:B6 | treatment:2Z | week:Wk 1	RAW_FILE_NAME=210611_se320.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_261	Strain:B6 | treatment:4X | week:Wk 1	RAW_FILE_NAME=210611_se321.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_262	Strain:B6 | treatment:3X | week:Wk 7	RAW_FILE_NAME=210611_se322.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_263	Strain:B6 | treatment:2X | week:Wk 1	RAW_FILE_NAME=210611_se323.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_264	Strain:B6 | treatment:3E | week:Wk 4	RAW_FILE_NAME=210611_se324.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC45	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se325.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC46	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se326.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_265	Strain:BC | treatment:3E | week:Wk 2	RAW_FILE_NAME=210611_se328.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_266	Strain:B6 | treatment:6C | week:Wk 4	RAW_FILE_NAME=210611_se329.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_267	Strain:B6 | treatment:2A | week:Wk 1	RAW_FILE_NAME=210611_se330.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_268	Strain:B6 | treatment:4E | week:Wk 1	RAW_FILE_NAME=210611_se331.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_269	Strain:BC | treatment:1A | week:Wk 2	RAW_FILE_NAME=210611_se332.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_270	Strain:BC | treatment:6A | week:Wk 4	RAW_FILE_NAME=210611_se333.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_271	Strain:B6 | treatment:3X | week:Wk 1	RAW_FILE_NAME=210611_se334.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_273	Strain:B6 | treatment:1X | week:Wk 7	RAW_FILE_NAME=210611_se335.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_274	Strain:B6 | treatment:5W | week:Wk 1	RAW_FILE_NAME=210611_se336.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_275	Strain:BC | treatment:5A | week:Wk 4	RAW_FILE_NAME=210611_se337.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC47	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se338.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC48	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se339.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_276	Strain:BC | treatment:4A | week:Wk 1	RAW_FILE_NAME=210611_se341.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_277	Strain:BC | treatment:2D | week:Wk 1	RAW_FILE_NAME=210611_se342.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_278	Strain:BC | treatment:6A | week:Wk 1	RAW_FILE_NAME=210611_se343.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_279	Strain:B6 | treatment:6E | week:Wk 1	RAW_FILE_NAME=210611_se344.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_280	Strain:BC | treatment:1C | week:Wk 4	RAW_FILE_NAME=210611_se345.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_281	Strain:BC | treatment:2W | week:Wk 5	RAW_FILE_NAME=210611_se346.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_282	Strain:B6 | treatment:3F | week:Wk 4	RAW_FILE_NAME=210611_se347.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_283	Strain:BC | treatment:1Z | week:Wk 5	RAW_FILE_NAME=210611_se348.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_285	Strain:BC | treatment:3A | week:Wk 4	RAW_FILE_NAME=210611_se349.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_286	Strain:B6 | treatment:4W | week:Wk 7	RAW_FILE_NAME=210611_se350.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC49	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se351.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC50	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se352.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_287	Strain:B6 | treatment:5Z | week:Wk 1	RAW_FILE_NAME=210611_se370.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_288	Strain:B6 | treatment:4Z | week:Wk 7	RAW_FILE_NAME=210611_se371.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_289	Strain:BC | treatment:3F | week:Wk 1	RAW_FILE_NAME=210611_se372.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_290	Strain:BC | treatment:5B | week:Wk 3	RAW_FILE_NAME=210611_se373.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_291	Strain:BC | treatment:1X | week:Wk 7	RAW_FILE_NAME=210611_se374.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_293	Strain:BC | treatment:1A | week:Wk 1	RAW_FILE_NAME=210611_se375.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_294	Strain:B6 | treatment:3W | week:Wk 7	RAW_FILE_NAME=210611_se376.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_295	Strain:BC | treatment:5W | week:Wk 5	RAW_FILE_NAME=210611_se377.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_296	Strain:BC | treatment:4B | week:Wk 1	RAW_FILE_NAME=210611_se378.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_297	Strain:BC | treatment:4Y | week:Wk 1	RAW_FILE_NAME=210611_se379.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC53	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se380.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC54	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se381.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_298	Strain:BC | treatment:4C | week:Wk 1	RAW_FILE_NAME=210611_se383.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_299	Strain:BC | treatment:5Y | week:Wk 7	RAW_FILE_NAME=210611_se384.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_300	Strain:BC | treatment:3A | week:Wk 2	RAW_FILE_NAME=210611_se385.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_301	Strain:BC | treatment:5D | week:Wk 4	RAW_FILE_NAME=210611_se386.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_302	Strain:BC | treatment:1W | week:Wk 3	RAW_FILE_NAME=210611_se387.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_303	Strain:BC | treatment:4F | week:Wk 3	RAW_FILE_NAME=210611_se388.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_304	Strain:B6 | treatment:6Z | week:Wk 1	RAW_FILE_NAME=210611_se389.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_305	Strain:BC | treatment:4W | week:Wk 5	RAW_FILE_NAME=210611_se390.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_306	Strain:B6 | treatment:5Y | week:Wk 7	RAW_FILE_NAME=210611_se391.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_308	Strain:B6 | treatment:1Z | week:Wk 1	RAW_FILE_NAME=210611_se392.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC55	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se393.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC56	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se394.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_309	Strain:BC | treatment:4B | week:Wk 3	RAW_FILE_NAME=210611_se396.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_310	Strain:BC | treatment:3W | week:Wk 1	RAW_FILE_NAME=210611_se397.raw
SUBJECT_SAMPLE_FACTORS           	-	S_21_0066_311	Strain:B6 | treatment:5W | week:Wk 7	RAW_FILE_NAME=210611_se398.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC57	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se399.raw
SUBJECT_SAMPLE_FACTORS           	-	iQC58	Strain:- | treatment:- | week:-	RAW_FILE_NAME=210611_se400.raw
#COLLECTION
CO:COLLECTION_SUMMARY            	Fecal samples were collected on different timepoints during sensitization of the
CO:COLLECTION_SUMMARY            	mice. Samples were freeze-dried within one week after collection and stored at
CO:COLLECTION_SUMMARY            	-80° awaiting analysis
CO:SAMPLE_TYPE                   	Feces
CO:COLLECTION_METHOD             	Spontaneous defecation
CO:COLLECTION_FREQUENCY          	1x week
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	Two different mice strains and two different protocols were tested: BALB/c (BC)
TR:TREATMENT_SUMMARY             	mice and C57Bl/6 (B6) mice. The first sensitization protocol was based on
TR:TREATMENT_SUMMARY             	Adel-Patient et al., in which 4-week-old mice received oral gavages containing
TR:TREATMENT_SUMMARY             	ß-lactoglobulin (BLG) and cholera toxin (CT) once a week for 6 weeks 20
TR:TREATMENT_SUMMARY             	(treatment W), the control groups received either BLG in PBS (X), CT in PBS (Y)
TR:TREATMENT_SUMMARY             	or PBS alone (Z). The second protocol was adapted from Shindo et al. and
TR:TREATMENT_SUMMARY             	included intragastrical administration of BLG in PBS or oil emulsion, followed
TR:TREATMENT_SUMMARY             	by intraperitoneal injection of sodium salicylate (SA). The latter protocol was
TR:TREATMENT_SUMMARY             	performed 5x a week for 3 weeks. Group A received BLG in PBS + SA, group B BLG
TR:TREATMENT_SUMMARY             	in oil + SA, group C BLG in PBS + PBS, group D BLG in oil + PBS, group E and F
TR:TREATMENT_SUMMARY             	were vehicle controls with group E receiving PBS + SA, and group F oil + SA
TR:TREATMENT_COMPOUND            	Beta-lactoglobulin, cholera toxin
TR:TREATMENT_ROUTE               	oral gavage, intraperitoneal injection
TR:TREATMENT_DOSE                	1 mg ß-lactoglobulin (BLG) per g body weight mixed with 10 µg of cholera toxin
TR:TREATMENT_DOSE                	(CT) in 0.1 mL PBS, or 1 mg BLG in 0.1 mL individual antigen solution (vehicle),
TR:TREATMENT_DOSE                	which was either PBS or an oil emulsion (saline, linoleic acid, and lecithin at
TR:TREATMENT_DOSE                	5:4:1 by volume). Intraperitoneal administration of 0.1 mL PBS or PBS containing
TR:TREATMENT_DOSE                	0.2 mg sodium salicylate (SA)
TR:TREATMENT_DOSEDURATION        	3 or 7 weeks
TR:TREATMENT_VEHICLE             	PBS or oil emulsion
TR:ANIMAL_ANESTHESIA             	CO
TR:ANIMAL_FASTING                	Evening before antigen challenge
TR:ANIMAL_ENDP_EUTHANASIA        	cervical dislocation
TR:ANIMAL_ENDP_CLINICAL_SIGNS    	severe anaphylaxis
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	To extract the polar fecal metabolome, 200 mg of lyophilized homogenized feces
SP:SAMPLEPREP_SUMMARY            	was dissolved in 4 mL of ultrapure water, after the addition of 100 mL ISTD
SP:SAMPLEPREP_SUMMARY            	mixture (25 ng mL&1 of Dvaline- d8 and L-alanine-d3). Subsequent to 30 s of
SP:SAMPLEPREP_SUMMARY            	thorough vortexing, 1 mL of an ice-cold methanol and ultrapure water (80:20, v/
SP:SAMPLEPREP_SUMMARY            	v) mixture was added. The supernatant of the solid-liquid extraction was
SP:SAMPLEPREP_SUMMARY            	collected after 1 min of vortexing and 10 min of rotation, followed by a 10-min
SP:SAMPLEPREP_SUMMARY            	centrifugation step (13 300 x g, at 4 %C). Next, the extract was passed over a
SP:SAMPLEPREP_SUMMARY            	polyamide filter (diameter of 25mm and pore size of 0.45 mm) (Machery-Nagel,
SP:SAMPLEPREP_SUMMARY            	Düren, Germany), diluted (1:3) with ultrapure water and transferred to a glass
SP:SAMPLEPREP_SUMMARY            	HPLCvial.
SP:PROCESSING_STORAGE_CONDITIONS 	-80℃
SP:SAMPLE_SPIKING                	Internal standard
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Thermo Dionex Ultimate 3000
CH:COLUMN_NAME                   	Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)
CH:SOLVENT_A                     	100% water; 0.1% formic acid
CH:SOLVENT_B                     	100% acetonitrile; 0.1% formic acid
CH:FLOW_GRADIENT                 	A gradient profile with following proportions (v/v) of solvent A was applied:
CH:FLOW_GRADIENT                 	0e1.5 min at 98%, 1.5e7.0 min from 98% to 75%, 7.0e8.0 min from 75% to 40%,
CH:FLOW_GRADIENT                 	8.0e12.0 min from 40% to 5%, 12.0e14.0 min at 5%, 14.0e14.1 min from 5 to 98%,
CH:FLOW_GRADIENT                 	followed by 4.0 min of re-equilibration.
CH:FLOW_RATE                     	0.4 mL/min
CH:COLUMN_TEMPERATURE            	45
CH:INTERNAL_STANDARD             	L-alanine-d3, D-valine-d8
CH:SAMPLE_INJECTION              	10 uL
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	UNSPECIFIED
MS:MS_COMMENTS                   	MS acquisition: Xcalibur Targeted data processing: Xcalibur Untargeted data
MS:MS_COMMENTS                   	acquisition: Compound Discoverer, R studio
MS:MS_RESULTS_FILE               	ST002944_AN004829_Results.txt	UNITS:a.u.	Has m/z:Neutral masses	Has RT:Yes	RT units:Minutes
#END