#METABOLOMICS WORKBENCH Yuanye_Chi_20231005_082718 DATATRACK_ID:4376 STUDY_ID:ST002964 ANALYSIS_ID:AN004866 PROJECT_ID:PR001844
VERSION             	1
CREATED_ON             	November 2, 2023, 3:36 pm
#PROJECT
PR:PROJECT_TITLE                 	Molecular Phenotypes of Null Alleles in Cells
PR:PROJECT_SUMMARY               	The 2020 NHGRI Strategic Vision laid out a set of “bold predictions for human
PR:PROJECT_SUMMARY               	genomics by 2030” including elucidating the biological function(s) of each
PR:PROJECT_SUMMARY               	human gene. The Molecular Phenotypes of Null Alleles in Cells (MorPhiC) seeks to
PR:PROJECT_SUMMARY               	address this element of the strategic vision. Through the comprehensive
PR:PROJECT_SUMMARY               	generation of null alleles for every human gene and then cataloging the
PR:PROJECT_SUMMARY               	resulting molecular and cellular phenotypes, the mechanisms that relate gene
PR:PROJECT_SUMMARY               	function to observed phenotypes can be determined. Furthermore, the resulting
PR:PROJECT_SUMMARY               	catalog of knockouts and phenotypes will be made available for broader use by
PR:PROJECT_SUMMARY               	the biomedical community. Although multiple approaches can be leveraged to
PR:PROJECT_SUMMARY               	measure molecular and cellular phenotypes resulting from gene knockouts,
PR:PROJECT_SUMMARY               	metabolomics and lipidomics (i.e., biochemical phenotyping) provides an avenue
PR:PROJECT_SUMMARY               	to understand the link between gene function and phenotypes at a molecular
PR:PROJECT_SUMMARY               	level. This project consists of studies performed to biochemical phenotype of
PR:PROJECT_SUMMARY               	cell lines and other samples generated as part of MorPhiC. Resources: 1.
PR:PROJECT_SUMMARY               	https://www.nih.gov/news-events/news-releases/nih-initiative-systematically-investigate-establish-function-every-human-gene
PR:PROJECT_SUMMARY               	2.
PR:PROJECT_SUMMARY               	https://www.genome.gov/research-funding/Funded-Programs-Projects/Molecular-Phenotypes-of-Null-Alleles-in-Cells
PR:INSTITUTE                     	The Jackson Laboratory for Genomic Medicine
PR:LABORATORY                    	Shuzhao Li Lab
PR:LAST_NAME                     	Chi
PR:FIRST_NAME                    	Yuanye
PR:ADDRESS                       	10 Discovery Dr, Farmington, CT
PR:EMAIL                         	yuanye.chi@jax.org
PR:PHONE                         	3395456866
#STUDY
ST:STUDY_TITLE                   	Comprehensive Untargeted LC-MS Metabolomics Analysis of CYP19A1/PRLR/PTN
ST:STUDY_TITLE                   	Knockout iPSCs
ST:STUDY_SUMMARY                 	Human induced pluripotent stem cells (hIPSCs) are a key tool for biomedical
ST:STUDY_SUMMARY                 	research. In this study, human induced pluripotent stem cells of the KOLF2.1
ST:STUDY_SUMMARY                 	lineage were generated with null allele mutations for CYP19A1, PRLR, and PTN.
ST:STUDY_SUMMARY                 	These cell lines were cultured on several types of media with and without
ST:STUDY_SUMMARY                 	various additives and were differentiated into primitive syncytium. Biochemical
ST:STUDY_SUMMARY                 	phenotyping, i.e., untargeted metabolomics and lipidomics profiling, was
ST:STUDY_SUMMARY                 	performed on both differentiated and undifferentiated cells using a Thermo
ST:STUDY_SUMMARY                 	Scientific Q Exactive HF-X Mass Spectrometer coupled to a Thermo Scientific
ST:STUDY_SUMMARY                 	Transcend LX-2 Duo UHPLC system, equipped with an HES-II ionization source,
ST:STUDY_SUMMARY                 	using both positive and negative ionization modes. This dataset, combined with
ST:STUDY_SUMMARY                 	the other -omics level data generated through MorPhiC on these cell lines,
ST:STUDY_SUMMARY                 	provides a molecular foundation for understanding the implications of specific
ST:STUDY_SUMMARY                 	gene deletions in these cell lines and their derivative cell types (e.g.,
ST:STUDY_SUMMARY                 	primitive syncytium). This study includes data generated from the cell pellets
ST:STUDY_SUMMARY                 	of the above cell lines. This study was funded, in part, through UM1HG012651
ST:STUDY_SUMMARY                 	which established the JAX MorPhiC Center, a MorPhiC Phase 1 Data Production
ST:STUDY_SUMMARY                 	Research and Development Center at the Jackson Laboratory for Genomic Medicine.
ST:INSTITUTE                     	The Jackson Laboratory for Genomic Medicine
ST:LAST_NAME                     	Chi
ST:FIRST_NAME                    	Yuanye
ST:ADDRESS                       	10 Discovery Dr, Farmington, CT
ST:EMAIL                         	yuanye.chi@jax.org
ST:PHONE                         	3395456866
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	Blank_HILICneg_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_HILICneg_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_HILICpos_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_HILICpos_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_IS_HILICneg_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_IS_HILICneg_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_IS_HILICneg_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_IS_HILICneg_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_IS_HILICpos_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_IS_HILICpos_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_IS_HILICpos_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_IS_HILICpos_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_IS_RPneg_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_IS_RPneg_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_IS_RPneg_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_IS_RPneg_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_IS_RPpos_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_IS_RPpos_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_IS_RPpos_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_IS_RPpos_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_RPneg_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_RPneg_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_RPpos_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_RPpos_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_1_HILICneg	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_1_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_1_HILICpos	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_1_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_1_Rpneg	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_1_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_1_Rppos	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_1_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_10_HILICneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_10_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_10_HILICpos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_10_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_10_Rpneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_10_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_10_Rppos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_10_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_11_HILICneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_11_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_11_HILICpos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_11_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_11_Rpneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_11_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_11_Rppos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_11_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_12_HILICneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_12_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_12_HILICpos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_12_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_12_Rpneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_12_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_12_Rppos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_12_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_13_HILICneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_13_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_13_HILICpos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_13_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_13_Rpneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_13_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_13_Rppos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_13_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_14_HILICneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_14_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_14_HILICpos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_14_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_14_Rpneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_14_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_14_Rppos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_14_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_15_HILICneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_15_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_15_HILICpos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_15_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_15_Rpneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_15_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_15_Rppos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_15_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_16_HILICneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_16_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_16_HILICpos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_16_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_16_Rpneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_16_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_16_Rppos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_16_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_17_HILICneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_17_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_17_HILICpos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_17_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_17_Rpneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_17_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_17_Rppos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_17_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_18_HILICneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_18_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_18_HILICpos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_18_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_18_Rpneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_18_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_18_Rppos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_18_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_19_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_19_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_19_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_19_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_19_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_19_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_19_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_19_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_2_HILICneg	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_2_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_2_HILICpos	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_2_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_2_Rpneg	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_2_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_2_Rppos	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_2_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_20_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_20_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_20_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_20_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_20_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_20_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_20_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_20_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_21_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_21_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_21_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_21_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_21_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_21_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_21_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_21_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_22_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_22_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_22_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_22_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_22_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_22_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_22_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_22_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_23_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_23_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_23_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_23_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_23_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_23_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_23_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_23_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_24_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_24_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_24_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_24_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_24_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_24_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_24_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Pellets_24_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_25_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_25_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_25_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_25_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_25_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_25_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_25_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_25_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_26_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_26_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_26_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_26_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_26_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_26_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_26_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_26_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_27_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_27_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_27_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_27_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_27_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_27_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_27_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_27_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_28_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_28_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_28_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_28_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_28_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_28_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_28_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_28_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_29_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_29_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_29_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_29_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_29_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_29_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_29_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_29_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_3_HILICneg	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_3_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_3_HILICpos	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_3_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_3_Rpneg	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_3_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_3_Rppos	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_3_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_30_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_30_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_30_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_30_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_30_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_30_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_30_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Pellets_30_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_31_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_31_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_31_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_31_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_31_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_31_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_31_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_31_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_32_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_32_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_32_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_32_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_32_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_32_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_32_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_32_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_33_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_33_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_33_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_33_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_33_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_33_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_33_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_33_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_34_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_34_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_34_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_34_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_34_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_34_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_34_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_34_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_35_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_35_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_35_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_35_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_35_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_35_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_35_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_35_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_36_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_36_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_36_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_36_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_36_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_36_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_36_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_36_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_37_HILICneg	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_37_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_37_HILICpos	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_37_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_37_Rpneg	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_37_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_37_Rppos	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_37_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_38_HILICneg	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_38_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_38_HILICpos	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_38_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_38_Rpneg	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_38_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_38_Rppos	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_38_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_39_HILICneg	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_39_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_39_HILICpos	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_39_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_39_Rpneg	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_39_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_39_Rppos	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_39_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_4_HILICneg	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_4_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_4_HILICpos	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_4_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_4_Rpneg	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_4_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_4_Rppos	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_4_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_40_HILICneg	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_40_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_40_HILICpos	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_40_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_40_Rpneg	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_40_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_40_Rppos	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_40_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_41_HILICneg	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_41_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_41_HILICpos	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_41_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_41_Rpneg	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_41_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_41_Rppos	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_41_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_42_HILICneg	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_42_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_42_HILICpos	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_42_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_42_Rpneg	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_42_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_42_Rppos	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_42_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_43_HILICneg	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_43_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_43_HILICpos	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_43_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_43_Rpneg	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_43_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_43_Rppos	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_43_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_44_HILICneg	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_44_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_44_HILICpos	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_44_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_44_Rpneg	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_44_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_44_Rppos	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_44_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_45_HILICneg	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_45_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_45_HILICpos	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_45_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_45_Rpneg	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_45_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_45_Rppos	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_45_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_46_HILICneg	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_46_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_46_HILICpos	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_46_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_46_Rpneg	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_46_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_46_Rppos	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_46_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_47_HILICneg	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_47_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_47_HILICpos	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_47_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_47_Rpneg	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_47_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_47_Rppos	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_47_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_48_HILICneg	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_48_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_48_HILICpos	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_48_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_48_Rpneg	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_48_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_48_Rppos	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_48_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_49_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_49_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_49_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_49_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_49_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_49_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_49_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_49_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_5_HILICneg	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_5_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_5_HILICpos	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_5_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_5_Rpneg	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_5_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_5_Rppos	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_5_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_50_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_50_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_50_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_50_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_50_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_50_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_50_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_50_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_51_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_51_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_51_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_51_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_51_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_51_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_51_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_51_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_52_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_52_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_52_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_52_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_52_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_52_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_52_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_52_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_53_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_53_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_53_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_53_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_53_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_53_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_53_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_53_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_54_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_54_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_54_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_54_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_54_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_54_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_54_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Pellets_54_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_6_HILICneg	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_6_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_6_HILICpos	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_6_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_6_Rpneg	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_6_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_6_Rppos	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_6_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_7_HILICneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_7_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_7_HILICpos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_7_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_7_Rpneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_7_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_7_Rppos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_7_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_8_HILICneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_8_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_8_HILICpos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_8_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_8_Rpneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_8_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_8_Rppos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_8_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_9_HILICneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_9_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_9_HILICpos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_9_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_9_Rpneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_9_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Pellets_9_Rppos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Pellets_9_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Pellets_HILICneg_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Pellets_HILICneg_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Pellets_HILICneg_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Pellets_HILICneg_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Pellets_HILICneg_3	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Pellets_HILICneg_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Pellets_HILICneg_DDA	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Pellets_HILICneg_DDA.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Pellets_HILICpos_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Pellets_HILICpos_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Pellets_HILICpos_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Pellets_HILICpos_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Pellets_HILICpos_3	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Pellets_HILICpos_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Pellets_HILICpos_DDA	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Pellets_HILICpos_DDA.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Pellets_RPneg_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Pellets_RPneg_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Pellets_RPneg_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Pellets_RPneg_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Pellets_RPneg_3	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Pellets_RPneg_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Pellets_RPneg_DDA	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Pellets_RPneg_DDA.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Pellets_RPpos_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Pellets_RPpos_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Pellets_RPpos_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Pellets_RPpos_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Pellets_RPpos_3	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Pellets_RPpos_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Pellets_RPpos_DDA	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Pellets_RPpos_DDA.raw
SUBJECT_SAMPLE_FACTORS           	-	Qstd_HILICneg_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Qstd_HILICneg_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Qstd_HILICneg_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Qstd_HILICneg_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Qstd_HILICpos_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Qstd_HILICpos_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Qstd_HILICpos_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Qstd_HILICpos_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Qstd_RPneg_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Qstd_RPneg_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Qstd_RPneg_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Qstd_RPneg_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Qstd_RPpos_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Qstd_RPpos_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Qstd_RPpos_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Qstd_RPpos_2.raw
#COLLECTION
CO:COLLECTION_SUMMARY            	Remove the supernatant and flash freeze with liquid nitrogen and store
CO:COLLECTION_SUMMARY            	immediately at -80C. Wash the wells once with PBS and then add accutase and
CO:COLLECTION_SUMMARY            	incubate at 37C for 7min. Aspirate the accutase and add stemflex media and with
CO:COLLECTION_SUMMARY            	a cell lifter scrape off the cells from the bottom of the well. Transfer the
CO:COLLECTION_SUMMARY            	cell lysate to an Eppendorf tube and wash twice with PBS by centrifuging at 300g
CO:COLLECTION_SUMMARY            	for 5min. Finally remove the PBS and flash freeze the pellet in liquid nitrogen
CO:COLLECTION_SUMMARY            	and store immediately at -80C.
CO:SAMPLE_TYPE                   	iPSC cells
#TREATMENT
TR:TREATMENT_SUMMARY             	Firstly seed 50,000 cells of the wild type and knockout lines per well in a 6
TR:TREATMENT_SUMMARY             	well plate in triplicate and incubate at 37C, after 24h add trophectoderm media
TR:TREATMENT_SUMMARY             	(TE) containing the growth factors BMP4 and SU5402 (day 0). Two days after (day
TR:TREATMENT_SUMMARY             	2), add the same media containing SU5402, BMP4 and A-83 and repeat this again
TR:TREATMENT_SUMMARY             	after 2 days (day 4). Finally, after another 2 days (day 6) remove the
TR:TREATMENT_SUMMARY             	differentiation media and add basal or complex media to the cells and incubate
TR:TREATMENT_SUMMARY             	for 24h at 37C.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	See from protocol file.
SP:SAMPLEPREP_PROTOCOL_FILENAME  	TwoPhaseCellExtract_Protocol_10_10_23_v3.pdf
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	Thermo Accucore HILIC (100 x 2.1mm,2.6um)
CH:SOLVENT_A                     	95% ACN/water (10 mM ammonium acetate, 0.1% acetic acid)
CH:SOLVENT_B                     	50% ACN/water (10 mM ammonium acetate, 0.1% acetic acid)
CH:FLOW_GRADIENT                 	From 0 to 0.20 minutes, B is 0% with a flow rate of 550 µL/min; it jumps to 98%
CH:FLOW_GRADIENT                 	by 8.75 minutes (still at 550 µL/min) and remains until 10 minutes; at 15
CH:FLOW_GRADIENT                 	minutes, B returns to 0% but the flow drops to 100 µL/min, then rises back to
CH:FLOW_GRADIENT                 	550 µL/min by 17 minutes, staying consistent until 20 minutes.
CH:FLOW_RATE                     	550->100->550µL/min
CH:COLUMN_TEMPERATURE            	45
CH:METHODS_FILENAME              	Regular_LC_MS_Protocol_10_25_23_v2.pdf
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:ANALYSIS_PROTOCOL_FILE        	Regular_LC_MS_Protocol_10_25_23_v2.pdf
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive HF-X Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	9. Configure the mass spectrometer: mass range, 66.7-1000 m/z; spray voltage,
MS:MS_COMMENTS                   	3500 V (ESI+), 2500 V (ESI-); sheath gas flow rate, 55 Arb; auxiliary gas flow
MS:MS_COMMENTS                   	rate, 15 Arb; sweep gas, 3 Arb; capillary temperature, 300 °C; auxiliary gas
MS:MS_COMMENTS                   	heater temperature, 425 °C; full scan mass resolution, 60,000 (MS1); AGC, 1e6;
MS:MS_COMMENTS                   	maximum injection time, 30 ms. Data dependent fragmentation (dd-MS/MS)
MS:MS_COMMENTS                   	parameters for each polarity as follows: isolation window (m/z), 1.2; stepped
MS:MS_COMMENTS                   	HCD collision energy (%), 20,40,80; dd-MS/MS resolution, 30,000; normalized AGC
MS:MS_COMMENTS                   	target, 1e5; maximum injection time (ms), 50; A full scan data-dependent MS2
MS:MS_COMMENTS                   	(ddMS2) method was utilized to collect MS2 spectra for identification of
MS:MS_COMMENTS                   	compounds.
MS:MS_RESULTS_FILE               	ST002964_AN004866_Results.txt	UNITS:peak intensity	Has m/z:Yes	Has RT:Yes	RT units:Seconds
#END