#METABOLOMICS WORKBENCH Yuanye_Chi_20231031_082120 DATATRACK_ID:4429 STUDY_ID:ST002965 ANALYSIS_ID:AN004871 PROJECT_ID:PR001844
VERSION             	1
CREATED_ON             	November 2, 2023, 10:59 am
#PROJECT
PR:PROJECT_TITLE                 	Molecular Phenotypes of Null Alleles in Cells(MorPhiC)
PR:PROJECT_SUMMARY               	The 2020 NHGRI Strategic Vision laid out a set of “bold predictions for human
PR:PROJECT_SUMMARY               	genomics by 2030” including elucidating the biological function(s) of each
PR:PROJECT_SUMMARY               	human gene. The Molecular Phenotypes of Null Alleles in Cells (MorPhiC) seeks to
PR:PROJECT_SUMMARY               	address this element of the strategic vision. Through the comprehensive
PR:PROJECT_SUMMARY               	generation of null alleles for every human gene and then cataloging the
PR:PROJECT_SUMMARY               	resulting molecular and cellular phenotypes, the mechanisms that relate gene
PR:PROJECT_SUMMARY               	function to observed phenotypes can be determined. Furthermore, the resulting
PR:PROJECT_SUMMARY               	catalog of knockouts and phenotypes will be made available for broader use by
PR:PROJECT_SUMMARY               	the biomedical community. Although multiple approaches can be leveraged to
PR:PROJECT_SUMMARY               	measure molecular and cellular phenotypes resulting from gene knockouts,
PR:PROJECT_SUMMARY               	metabolomics and lipidomics (i.e., biochemical phenotyping) provides an avenue
PR:PROJECT_SUMMARY               	to understand the link between gene function and phenotypes at a molecular
PR:PROJECT_SUMMARY               	level. This project consists of studies performed to biochemical phenotype of
PR:PROJECT_SUMMARY               	cell lines and other samples generated as part of MorPhiC. Resources: 1.
PR:PROJECT_SUMMARY               	https://www.nih.gov/news-events/news-releases/nih-initiative-systematically-investigate-establish-function-every-human-gene
PR:PROJECT_SUMMARY               	2.
PR:PROJECT_SUMMARY               	https://www.genome.gov/research-funding/Funded-Programs-Projects/Molecular-Phenotypes-of-Null-Alleles-in-Cells
PR:INSTITUTE                     	The Jackson Laboratory for Genomic Medicine
PR:LABORATORY                    	Shuzhao Li Lab
PR:LAST_NAME                     	Chi
PR:FIRST_NAME                    	Yuanye
PR:ADDRESS                       	10 Discovery Dr, Farmington, CT
PR:EMAIL                         	yuanye.chi@jax.org
PR:PHONE                         	3395456866
#STUDY
ST:STUDY_TITLE                   	Comprehensive Untargeted LC-MS Metabolomics Analysis of CYP19A1/PRLR/PTN
ST:STUDY_TITLE                   	Knockout iPSCs
ST:STUDY_SUMMARY                 	Human induced pluripotent stem cells (hIPSCs) are a key tool for biomedical
ST:STUDY_SUMMARY                 	research. In this study, human induced pluripotent stem cells of the KOLF2.1
ST:STUDY_SUMMARY                 	lineage were generated with null allele mutations for CYP19A1, PRLR, and PTN.
ST:STUDY_SUMMARY                 	These cell lines were cultured on several types of media with and without
ST:STUDY_SUMMARY                 	various additives and were differentiated into primitive syncytium. Biochemical
ST:STUDY_SUMMARY                 	phenotyping, i.e., untargeted metabolomics and lipidomics profiling, was
ST:STUDY_SUMMARY                 	performed on both differentiated and undifferentiated cells using a Thermo
ST:STUDY_SUMMARY                 	Scientific Q Exactive HF-X Mass Spectrometer coupled to a Thermo Scientific
ST:STUDY_SUMMARY                 	Transcend LX-2 Duo UHPLC system, equipped with an HES-II ionization source,
ST:STUDY_SUMMARY                 	using both positive and negative ionization modes. This dataset, combined with
ST:STUDY_SUMMARY                 	the other -omics level data generated through MorPhiC on these cell lines,
ST:STUDY_SUMMARY                 	provides a molecular foundation for understanding the implications of specific
ST:STUDY_SUMMARY                 	gene deletions in these cell lines and their derivative cell types (e.g.,
ST:STUDY_SUMMARY                 	primitive syncytium). This study includes data generated from the supernatant of
ST:STUDY_SUMMARY                 	the above cell lines. This study was funded, in part, through UM1HG012651 which
ST:STUDY_SUMMARY                 	established the JAX MorPhiC Center, a MorPhiC Phase 1 Data Production Research
ST:STUDY_SUMMARY                 	and Development Center at the Jackson Laboratory for Genomic Medicine.
ST:INSTITUTE                     	The Jackson Laboratory for Genomic Medicine
ST:LAST_NAME                     	Chi
ST:FIRST_NAME                    	Yuanye
ST:ADDRESS                       	10 Discovery Dr, Farmington, CT
ST:EMAIL                         	yuanye.chi@jax.org
ST:PHONE                         	3395456866
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	Blank_HILICneg_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_HILICneg_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_HILICpos_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_HILICpos_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_IS_HILICneg_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_IS_HILICneg_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_IS_HILICneg_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_IS_HILICneg_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_IS_HILICpos_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_IS_HILICpos_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_IS_HILICpos_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_IS_HILICpos_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_IS_RPneg_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_IS_RPneg_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_IS_RPneg_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_IS_RPneg_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_IS_RPpos_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_IS_RPpos_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_IS_RPpos_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_IS_RPpos_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_RPneg_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_RPneg_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Blank_RPpos_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Blank_RPpos_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_1_HILICneg	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_1_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_1_HILICpos	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_1_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_1_Rpneg	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_1_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_1_Rppos	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_1_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_10_HILICneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_10_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_10_HILICpos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_10_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_10_Rpneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_10_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_10_Rppos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_10_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_11_HILICneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_11_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_11_HILICpos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_11_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_11_Rpneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_11_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_11_Rppos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_11_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_12_HILICneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_12_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_12_HILICpos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_12_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_12_Rpneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_12_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_12_Rppos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_12_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_13_HILICneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_13_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_13_HILICpos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_13_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_13_Rpneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_13_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_13_Rppos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_13_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_14_HILICneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_14_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_14_HILICpos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_14_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_14_Rpneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_14_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_14_Rppos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_14_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_15_HILICneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_15_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_15_HILICpos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_15_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_15_Rpneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_15_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_15_Rppos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_15_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_16_HILICneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_16_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_16_HILICpos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_16_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_16_Rpneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_16_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_16_Rppos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_16_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_17_HILICneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_17_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_17_HILICpos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_17_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_17_Rpneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_17_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_17_Rppos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_17_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_18_HILICneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_18_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_18_HILICpos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_18_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_18_Rpneg	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_18_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_18_Rppos	Cell Line:prlr KO | Differentiation:ipsc | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_18_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_19_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_19_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_19_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_19_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_19_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_19_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_19_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_19_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_2_HILICneg	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_2_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_2_HILICpos	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_2_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_2_Rpneg	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_2_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_2_Rppos	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_2_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_20_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_20_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_20_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_20_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_20_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_20_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_20_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_20_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_21_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_21_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_21_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_21_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_21_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_21_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_21_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_21_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_22_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_22_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_22_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_22_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_22_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_22_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_22_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_22_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_23_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_23_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_23_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_23_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_23_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_23_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_23_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_23_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_24_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_24_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_24_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_24_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_24_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_24_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_24_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:dheas	RAW_FILE_NAME=Media_24_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_25_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_25_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_25_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_25_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_25_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_25_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_25_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_25_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_26_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_26_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_26_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_26_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_26_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_26_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_26_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_26_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_27_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_27_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_27_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_27_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_27_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_27_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_27_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_27_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_28_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_28_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_28_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_28_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_28_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_28_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_28_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_28_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_29_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_29_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_29_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_29_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_29_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_29_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_29_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_29_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_3_HILICneg	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_3_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_3_HILICpos	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_3_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_3_Rpneg	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_3_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_3_Rppos	Cell Line:wt | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_3_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_30_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_30_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_30_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_30_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_30_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_30_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_30_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:prolactin	RAW_FILE_NAME=Media_30_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_31_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_31_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_31_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_31_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_31_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_31_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_31_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_31_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_32_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_32_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_32_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_32_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_32_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_32_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_32_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_32_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_33_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_33_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_33_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_33_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_33_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_33_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_33_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_33_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_34_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_34_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_34_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_34_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_34_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_34_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_34_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_34_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_35_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_35_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_35_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_35_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_35_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_35_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_35_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_35_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_36_HILICneg	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_36_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_36_HILICpos	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_36_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_36_Rpneg	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_36_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_36_Rppos	Cell Line:prlr KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_36_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_37_HILICneg	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_37_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_37_HILICpos	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_37_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_37_Rpneg	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_37_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_37_Rppos	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_37_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_38_HILICneg	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_38_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_38_HILICpos	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_38_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_38_Rpneg	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_38_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_38_Rppos	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_38_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_39_HILICneg	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_39_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_39_HILICpos	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_39_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_39_Rpneg	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_39_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_39_Rppos	Cell Line:ptn KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_39_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_4_HILICneg	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_4_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_4_HILICpos	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_4_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_4_Rpneg	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_4_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_4_Rppos	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_4_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_40_HILICneg	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_40_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_40_HILICpos	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_40_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_40_Rpneg	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_40_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_40_Rppos	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_40_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_41_HILICneg	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_41_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_41_HILICpos	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_41_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_41_Rpneg	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_41_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_41_Rppos	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_41_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_42_HILICneg	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_42_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_42_HILICpos	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_42_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_42_Rpneg	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_42_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_42_Rppos	Cell Line:ptn KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_42_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_43_HILICneg	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_43_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_43_HILICpos	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_43_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_43_Rpneg	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_43_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_43_Rppos	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_43_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_44_HILICneg	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_44_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_44_HILICpos	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_44_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_44_Rpneg	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_44_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_44_Rppos	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_44_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_45_HILICneg	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_45_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_45_HILICpos	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_45_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_45_Rpneg	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_45_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_45_Rppos	Cell Line:wt | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_45_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_46_HILICneg	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_46_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_46_HILICpos	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_46_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_46_Rpneg	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_46_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_46_Rppos	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_46_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_47_HILICneg	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_47_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_47_HILICpos	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_47_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_47_Rpneg	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_47_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_47_Rppos	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_47_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_48_HILICneg	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_48_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_48_HILICpos	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_48_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_48_Rpneg	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_48_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_48_Rppos	Cell Line:wt | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_48_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_49_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_49_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_49_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_49_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_49_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_49_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_49_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_49_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_5_HILICneg	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_5_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_5_HILICpos	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_5_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_5_Rpneg	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_5_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_5_Rppos	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_5_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_50_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_50_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_50_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_50_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_50_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_50_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_50_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_50_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_51_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_51_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_51_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_51_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_51_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_51_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_51_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:basal | Additives:-	RAW_FILE_NAME=Media_51_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_52_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_52_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_52_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_52_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_52_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_52_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_52_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_52_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_53_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_53_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_53_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_53_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_53_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_53_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_53_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_53_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_54_HILICneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_54_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_54_HILICpos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_54_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_54_Rpneg	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_54_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_54_Rppos	Cell Line:cyp19a1 KO | Differentiation:differentiated | Media:complex | Additives:-	RAW_FILE_NAME=Media_54_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_6_HILICneg	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_6_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_6_HILICpos	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_6_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_6_Rpneg	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_6_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_6_Rppos	Cell Line:ptn KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_6_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_7_HILICneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_7_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_7_HILICpos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_7_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_7_Rpneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_7_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_7_Rppos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_7_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_8_HILICneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_8_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_8_HILICpos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_8_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_8_Rpneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_8_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_8_Rppos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_8_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_9_HILICneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_9_HILICneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_9_HILICpos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_9_HILICpos.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_9_Rpneg	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_9_Rpneg.raw
SUBJECT_SAMPLE_FACTORS           	-	Media_9_Rppos	Cell Line:cyp19a1 KO | Differentiation:ipsc | Media:basal | Additives:-	RAW_FILE_NAME=Media_9_Rppos.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Media_HILICneg_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Media_HILICneg_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Media_HILICneg_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Media_HILICneg_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Media_HILICneg_3	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Media_HILICneg_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Media_HILICneg_DDA	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Media_HILICneg_DDA.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Media_HILICpos_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Media_HILICpos_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Media_HILICpos_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Media_HILICpos_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Media_HILICpos_3	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Media_HILICpos_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Media_HILICpos_DDA	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Media_HILICpos_DDA.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Media_RPneg_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Media_RPneg_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Media_RPneg_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Media_RPneg_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Media_RPneg_3	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Media_RPneg_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Media_RPneg_DDA	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Media_RPneg_DDA.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Media_RPpos_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Media_RPpos_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Media_RPpos_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Media_RPpos_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Media_RPpos_3	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Media_RPpos_3.raw
SUBJECT_SAMPLE_FACTORS           	-	Pool_QC_Media_RPpos_DDA	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Pool_QC_Media_RPpos_DDA.raw
SUBJECT_SAMPLE_FACTORS           	-	Qstd_HILICneg_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Qstd_HILICneg_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Qstd_HILICneg_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Qstd_HILICneg_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Qstd_HILICpos_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Qstd_HILICpos_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Qstd_HILICpos_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Qstd_HILICpos_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Qstd_RPneg_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Qstd_RPneg_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Qstd_RPneg_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Qstd_RPneg_2.raw
SUBJECT_SAMPLE_FACTORS           	-	Qstd_RPpos_1	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Qstd_RPpos_1.raw
SUBJECT_SAMPLE_FACTORS           	-	Qstd_RPpos_2	Cell Line:- | Differentiation:- | Media:- | Additives:-	RAW_FILE_NAME=Qstd_RPpos_2.raw
#COLLECTION
CO:COLLECTION_SUMMARY            	Remove the supernatant and flash freeze with liquid nitrogen and store
CO:COLLECTION_SUMMARY            	immediately at -80C.
CO:SAMPLE_TYPE                   	iPSC cells
#TREATMENT
TR:TREATMENT_SUMMARY             	Firstly seed 50,000 cells of the wild type and knockout lines per well in a 6
TR:TREATMENT_SUMMARY             	well plate in triplicate and incubate at 37C, after 24h add trophectoderm media
TR:TREATMENT_SUMMARY             	(TE) containing the growth factors BMP4 and SU5402 (day 0). Two days after (day
TR:TREATMENT_SUMMARY             	2), add the same media containing SU5402, BMP4 and A-83 and repeat this again
TR:TREATMENT_SUMMARY             	after 2 days (day 4). Finally, after another 2 days (day 6) remove the
TR:TREATMENT_SUMMARY             	differentiation media and add basal or complex media to the cells and incubate
TR:TREATMENT_SUMMARY             	for 24h at 37C.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	See from protocol file.
SP:SAMPLEPREP_PROTOCOL_FILENAME  	TwoPhaseSupernatantExtract_Protocol_10_10_23_v3.pdf
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	Hypersil GOLD (50 x 2.1mm, 3um)
CH:SOLVENT_A                     	60% ACN/water (10 mM ammonium formate, 0.1% formic acid)
CH:SOLVENT_B                     	90% 2-propanol/ACN (10 mM ammonium formate, 0.1% formic acid)
CH:FLOW_GRADIENT                 	From 0 to 0.01 minutes, B is 15% at a flow rate of 400 µL/min; by 2.01 minutes,
CH:FLOW_GRADIENT                 	it's 30%, reaching 48% at 2.51 minutes, then 82% by 11 minutes, and it sharply
CH:FLOW_GRADIENT                 	rises to 99% at 11.50 minutes holding until 16.50 minutes when it peaks at 100%;
CH:FLOW_GRADIENT                 	from 17.50 to 20 minutes, it drops back to 15%, with the flow rate consistently
CH:FLOW_GRADIENT                 	maintained at 400 µL/min throughout.
CH:FLOW_RATE                     	400µL/min
CH:COLUMN_TEMPERATURE            	45
CH:METHODS_FILENAME              	Regular_LC_MS_Protocol_10_25_23_v2.pdf
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:ANALYSIS_PROTOCOL_FILE        	Regular_LC_MS_Protocol_10_25_23_v2.pdf
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive HF-X Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	9. Configure the mass spectrometer: mass range, 66.7-1000 m/z; spray voltage,
MS:MS_COMMENTS                   	3500 V (ESI+), 2500 V (ESI-); sheath gas flow rate, 55 Arb; auxiliary gas flow
MS:MS_COMMENTS                   	rate, 15 Arb; sweep gas, 3 Arb; capillary temperature, 300 °C; auxiliary gas
MS:MS_COMMENTS                   	heater temperature, 425 °C; full scan mass resolution, 60,000 (MS1); AGC, 1e6;
MS:MS_COMMENTS                   	maximum injection time, 30 ms. Data dependent fragmentation (dd-MS/MS)
MS:MS_COMMENTS                   	parameters for each polarity as follows: isolation window (m/z), 1.2; stepped
MS:MS_COMMENTS                   	HCD collision energy (%), 20,40,80; dd-MS/MS resolution, 30,000; normalized AGC
MS:MS_COMMENTS                   	target, 1e5; maximum injection time (ms), 50; A full scan data-dependent MS2
MS:MS_COMMENTS                   	(ddMS2) method was utilized to collect MS2 spectra for identification of
MS:MS_COMMENTS                   	compounds.
MS:MS_RESULTS_FILE               	ST002965_AN004871_Results.txt	UNITS:peak intensity	Has m/z:Yes	Has RT:Yes	RT units:Seconds
#END