#METABOLOMICS WORKBENCH engelmab_20231212_234250 DATATRACK_ID:4504 STUDY_ID:ST003011 ANALYSIS_ID:AN004942 PROJECT_ID:PR001874
VERSION             	1
CREATED_ON             	December 13, 2023, 3:06 am
#PROJECT
PR:PROJECT_TITLE                 	Effects of the human intestinal bacterium Eggerthella lenta in gut of
PR:PROJECT_TITLE                 	gnotobiotic mice
PR:PROJECT_SUMMARY               	In this work, we used the minimal consortium Oligo-Mouse Microbiota (OMM)12 to
PR:PROJECT_SUMMARY               	study the function of Coriobacteriia under defined conditions in gnotobiotic
PR:PROJECT_SUMMARY               	mice. OMM12 mice with or without addition of the dominant gut bacterium
PR:PROJECT_SUMMARY               	Eggerthella lenta were fed with diets varying in fat content or supplemented
PR:PROJECT_SUMMARY               	with primary bile acids.
PR:INSTITUTE                     	Helmholtz Centre for Environmental Research
PR:DEPARTMENT                    	Molecular Systems Biology
PR:LAST_NAME                     	Engelmann
PR:FIRST_NAME                    	Beatrice
PR:ADDRESS                       	Permoserstrasse 15, 04318 Leipzig, Germany
PR:EMAIL                         	beatrice.engelmann@ufz.de
PR:PHONE                         	00493411099
#STUDY
ST:STUDY_TITLE                   	Untargeted metabolomics analysis of murine colon content
ST:STUDY_SUMMARY                 	we used the minimal consortium Oligo-Mouse Microbiota (OMM)12 to study the
ST:STUDY_SUMMARY                 	function of Coriobacteriia under defined conditions in gnotobiotic mice. OMM12
ST:STUDY_SUMMARY                 	mice with or without addition of the dominant gut bacterium Eggerthella lenta
ST:STUDY_SUMMARY                 	were fed with diets varying in fat content or supplemented with primary bile
ST:STUDY_SUMMARY                 	acids.
ST:INSTITUTE                     	Helmholtz Centre for Environmental Research
ST:DEPARTMENT                    	Molecular Systems Biology
ST:LAST_NAME                     	Engelmann
ST:FIRST_NAME                    	Beatrice
ST:ADDRESS                       	Permoserstrasse 15, 04318 Leipzig, Germany
ST:EMAIL                         	beatrice.engelmann@ufz.de
ST:PHONE                         	+493411099
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	2_O_HFD	2_O_HFD	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=2_O_HFD.mzML
SUBJECT_SAMPLE_FACTORS           	3_O_HFD	3_O_HFD	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=3_O_HFD.mzML
SUBJECT_SAMPLE_FACTORS           	4_O_HFD	4_O_HFD	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=4_O_HFD.mzML
SUBJECT_SAMPLE_FACTORS           	5_O_HFD	5_O_HFD	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=5_O_HFD.mzML
SUBJECT_SAMPLE_FACTORS           	6_O_HFD	6_O_HFD	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=6_O_HFD.mzML
SUBJECT_SAMPLE_FACTORS           	7_O_HFD	7_O_HFD	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=7_O_HFD.mzML
SUBJECT_SAMPLE_FACTORS           	8_O_BA	8_O_BA	Diet:BA | colonization:OligoMM	RAW_FILE_NAME=8_O_BA.mzML
SUBJECT_SAMPLE_FACTORS           	9_O_HFD.BA	9_O_HFD.BA	Diet:HFD.BA | colonization:OligoMM	RAW_FILE_NAME=9_O_HFD.BA.mzML
SUBJECT_SAMPLE_FACTORS           	11_O_BA	11_O_BA	Diet:BA | colonization:OligoMM	RAW_FILE_NAME=11_O_BA.mzML
SUBJECT_SAMPLE_FACTORS           	13_O_CD	13_O_CD	Diet:CD | colonization:OligoMM	RAW_FILE_NAME=13_O_CD.mzML
SUBJECT_SAMPLE_FACTORS           	14_O_CD	14_O_CD	Diet:CD | colonization:OligoMM	RAW_FILE_NAME=14_O_CD.mzML
SUBJECT_SAMPLE_FACTORS           	17_Corio.O_CD	17_Corio.O_CD	Diet:CD | colonization:Corio_OligoMM	RAW_FILE_NAME=17_Corio.O_CD.mzML
SUBJECT_SAMPLE_FACTORS           	19_Corio.O_CD	19_Corio.O_CD	Diet:CD | colonization:Corio_OligoMM	RAW_FILE_NAME=19_Corio.O_CD.mzML
SUBJECT_SAMPLE_FACTORS           	20_Corio.O_CD	20_Corio.O_CD	Diet:CD | colonization:Corio_OligoMM	RAW_FILE_NAME=20_Corio.O_CD.mzML
SUBJECT_SAMPLE_FACTORS           	21_Corio.O_BA	21_Corio.O_BA	Diet:BA | colonization:Corio_OligoMM	RAW_FILE_NAME=21_Corio.O_BA.mzML
SUBJECT_SAMPLE_FACTORS           	22_Corio.O_BA	22_Corio.O_BA	Diet:BA | colonization:Corio_OligoMM	RAW_FILE_NAME=22_Corio.O_BA.mzML
SUBJECT_SAMPLE_FACTORS           	23_Corio.O_BA	23_Corio.O_BA	Diet:BA | colonization:Corio_OligoMM	RAW_FILE_NAME=23_Corio.O_BA.mzML
SUBJECT_SAMPLE_FACTORS           	24_Corio.O_BA	24_Corio.O_BA	Diet:BA | colonization:Corio_OligoMM	RAW_FILE_NAME=24_Corio.O_BA.mzML
SUBJECT_SAMPLE_FACTORS           	25_Corio.O_HFD	25_Corio.O_HFD	Diet:HFD | colonization:Corio_OligoMM	RAW_FILE_NAME=25_Corio.O_HFD.mzML
SUBJECT_SAMPLE_FACTORS           	27_Corio.O_HFD	27_Corio.O_HFD	Diet:HFD | colonization:Corio_OligoMM	RAW_FILE_NAME=27_Corio.O_HFD.mzML
SUBJECT_SAMPLE_FACTORS           	28_Corio.O_HFD	28_Corio.O_HFD	Diet:HFD | colonization:Corio_OligoMM	RAW_FILE_NAME=28_Corio.O_HFD.mzML
SUBJECT_SAMPLE_FACTORS           	29_Corio.O_HFD.BA	29_Corio.O_HFD.BA	Diet:HFD.BA | colonization:Corio_OligoMM	RAW_FILE_NAME=29_Corio.O_HFD.BA.mzML
SUBJECT_SAMPLE_FACTORS           	30_Corio.O_HFD.BA	30_Corio.O_HFD.BA	Diet:HFD.BA | colonization:Corio_OligoMM	RAW_FILE_NAME=30_Corio.O_HFD.BA.mzML
SUBJECT_SAMPLE_FACTORS           	31_Corio.O_HFD.BA	31_Corio.O_HFD.BA	Diet:HFD.BA | colonization:Corio_OligoMM	RAW_FILE_NAME=31_Corio.O_HFD.BA.mzML
SUBJECT_SAMPLE_FACTORS           	32_Corio.O_HFD.BA	32_Corio.O_HFD.BA	Diet:HFD.BA | colonization:Corio_OligoMM	RAW_FILE_NAME=32_Corio.O_HFD.BA.mzML
SUBJECT_SAMPLE_FACTORS           	33_Corio.O_CD	33_Corio.O_CD	Diet:CD | colonization:Corio_OligoMM	RAW_FILE_NAME=33_Corio.O_CD.mzML
SUBJECT_SAMPLE_FACTORS           	34_Corio.O_CD	34_Corio.O_CD	Diet:CD | colonization:Corio_OligoMM	RAW_FILE_NAME=34_Corio.O_CD.mzML
SUBJECT_SAMPLE_FACTORS           	35_Corio.O_CD	35_Corio.O_CD	Diet:CD | colonization:Corio_OligoMM	RAW_FILE_NAME=35_Corio.O_CD.mzML
SUBJECT_SAMPLE_FACTORS           	36_O_CD	36_O_CD	Diet:CD | colonization:OligoMM	RAW_FILE_NAME=36_O_CD.mzML
SUBJECT_SAMPLE_FACTORS           	37_O_CD	37_O_CD	Diet:CD | colonization:OligoMM	RAW_FILE_NAME=37_O_CD.mzML
SUBJECT_SAMPLE_FACTORS           	38_O_CD	38_O_CD	Diet:CD | colonization:OligoMM	RAW_FILE_NAME=38_O_CD.mzML
SUBJECT_SAMPLE_FACTORS           	39_O_HFD	39_O_HFD	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=39_O_HFD.mzML
SUBJECT_SAMPLE_FACTORS           	40_O_HFD	40_O_HFD	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=40_O_HFD.mzML
SUBJECT_SAMPLE_FACTORS           	41_O_HFD	41_O_HFD	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=41_O_HFD.mzML
SUBJECT_SAMPLE_FACTORS           	42_O_BA	42_O_BA	Diet:BA | colonization:OligoMM	RAW_FILE_NAME=42_O_BA.mzML
SUBJECT_SAMPLE_FACTORS           	43_Corio.O_BA	43_Corio.O_BA	Diet:BA | colonization:Corio_OligoMM	RAW_FILE_NAME=43_Corio.O_BA.mzML
SUBJECT_SAMPLE_FACTORS           	44_Corio.O_BA	44_Corio.O_BA	Diet:BA | colonization:Corio_OligoMM	RAW_FILE_NAME=44_Corio.O_BA.mzML
SUBJECT_SAMPLE_FACTORS           	45_Corio.O_BA	45_Corio.O_BA	Diet:BA | colonization:Corio_OligoMM	RAW_FILE_NAME=45_Corio.O_BA.mzML
SUBJECT_SAMPLE_FACTORS           	46_O_CD	46_O_CD	Diet:CD | colonization:OligoMM	RAW_FILE_NAME=46_O_CD.mzML
SUBJECT_SAMPLE_FACTORS           	47_Corio.O_HFD.BA	47_Corio.O_HFD.BA	Diet:HFD.BA | colonization:Corio_OligoMM	RAW_FILE_NAME=47_Corio.O_HFD.BA.mzML
SUBJECT_SAMPLE_FACTORS           	48_Corio.O_HFD.BA	48_Corio.O_HFD.BA	Diet:HFD.BA | colonization:Corio_OligoMM	RAW_FILE_NAME=48_Corio.O_HFD.BA.mzML
SUBJECT_SAMPLE_FACTORS           	49_Corio.O_HFD.BA	49_Corio.O_HFD.BA	Diet:HFD.BA | colonization:Corio_OligoMM	RAW_FILE_NAME=49_Corio.O_HFD.BA.mzML
SUBJECT_SAMPLE_FACTORS           	50_Corio.O_HFD	50_Corio.O_HFD	Diet:HFD | colonization:Corio_OligoMM	RAW_FILE_NAME=50_Corio.O_HFD.mzML
SUBJECT_SAMPLE_FACTORS           	51_Corio.O_HFD	51_Corio.O_HFD	Diet:HFD | colonization:Corio_OligoMM	RAW_FILE_NAME=51_Corio.O_HFD.mzML
SUBJECT_SAMPLE_FACTORS           	52_Corio.O_HFD	52_Corio.O_HFD	Diet:HFD | colonization:Corio_OligoMM	RAW_FILE_NAME=52_Corio.O_HFD.mzML
SUBJECT_SAMPLE_FACTORS           	53_Corio.O_HFD	53_Corio.O_HFD	Diet:HFD | colonization:Corio_OligoMM	RAW_FILE_NAME=53_Corio.O_HFD.mzML
SUBJECT_SAMPLE_FACTORS           	54_O_BA	54_O_BA	Diet:BA | colonization:OligoMM	RAW_FILE_NAME=54_O_BA.mzML
SUBJECT_SAMPLE_FACTORS           	55_O_BA	55_O_BA	Diet:BA | colonization:OligoMM	RAW_FILE_NAME=55_O_BA.mzML
SUBJECT_SAMPLE_FACTORS           	56_O_BA	56_O_BA	Diet:BA | colonization:OligoMM	RAW_FILE_NAME=56_O_BA.mzML
SUBJECT_SAMPLE_FACTORS           	57_O_BA	57_O_BA	Diet:BA | colonization:OligoMM	RAW_FILE_NAME=57_O_BA.mzML
SUBJECT_SAMPLE_FACTORS           	58_O_HFD.BA	58_O_HFD.BA	Diet:HFD.BA | colonization:OligoMM	RAW_FILE_NAME=58_O_HFD.BA.mzML
SUBJECT_SAMPLE_FACTORS           	59_O_HFD.BA	59_O_HFD.BA	Diet:HFD.BA | colonization:OligoMM	RAW_FILE_NAME=59_O_HFD.BA.mzML
SUBJECT_SAMPLE_FACTORS           	60_O_HFD.BA	60_O_HFD.BA	Diet:HFD.BA | colonization:OligoMM	RAW_FILE_NAME=60_O_HFD.BA.mzML
SUBJECT_SAMPLE_FACTORS           	61_O_HFD.BA	61_O_HFD.BA	Diet:HFD.BA | colonization:OligoMM	RAW_FILE_NAME=61_O_HFD.BA.mzML
SUBJECT_SAMPLE_FACTORS           	422_Corio.O_BA	422_Corio.O_BA	Diet:BA | colonization:Corio_OligoMM	RAW_FILE_NAME=422_Corio.O_BA.mzML
SUBJECT_SAMPLE_FACTORS           	462_Corio.O_HFD.BA	462_Corio.O_HFD.BA	Diet:HFD.BA | colonization:Corio_OligoMM	RAW_FILE_NAME=462_Corio.O_HFD.BA.mzML
SUBJECT_SAMPLE_FACTORS           	11_6_O_BA	11_6_O_BA	Diet:BA | colonization:OligoMM	RAW_FILE_NAME=11+6_O_BA.mzML
SUBJECT_SAMPLE_FACTORS           	2_O_HFD	2_O_HFD_pos	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=2_O_HFD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	3_O_HFD	3_O_HFD_pos	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=3_O_HFD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	4_O_HFD	4_O_HFD_pos	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=4_O_HFD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	5_O_HFD	5_O_HFD_pos	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=5_O_HFD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	6_O_HFD	6_O_HFD_pos	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=6_O_HFD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	7_O_HFD	7_O_HFD_pos	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=7_O_HFD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	8_O_BA	8_O_BA_pos	Diet:BA | colonization:OligoMM	RAW_FILE_NAME=8_O_BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	9_O_HFD.BA	9_O_HFD.BA_pos	Diet:HFD.BA | colonization:OligoMM	RAW_FILE_NAME=9_O_HFD.BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	11_O_BA	11_O_BA_pos	Diet:BA | colonization:OligoMM	RAW_FILE_NAME=11_O_BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	13_O_CD	13_O_CD_pos	Diet:CD | colonization:OligoMM	RAW_FILE_NAME=13_O_CD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	14_O_CD	14_O_CD_pos	Diet:CD | colonization:OligoMM	RAW_FILE_NAME=14_O_CD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	17_Corio.O_CD	17_Corio.O_CD_pos	Diet:CD | colonization:Corio_OligoMM	RAW_FILE_NAME=17_Corio.O_CD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	19_Corio.O_CD	19_Corio.O_CD_pos	Diet:CD | colonization:Corio_OligoMM	RAW_FILE_NAME=19_Corio.O_CD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	20_Corio.O_CD	20_Corio.O_CD_pos	Diet:CD | colonization:Corio_OligoMM	RAW_FILE_NAME=20_Corio.O_CD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	21_Corio.O_BA	21_Corio.O_BA_pos	Diet:BA | colonization:Corio_OligoMM	RAW_FILE_NAME=21_Corio.O_BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	22_Corio.O_BA	22_Corio.O_BA_pos	Diet:BA | colonization:Corio_OligoMM	RAW_FILE_NAME=22_Corio.O_BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	23_Corio.O_BA	23_Corio.O_BA_pos	Diet:BA | colonization:Corio_OligoMM	RAW_FILE_NAME=23_Corio.O_BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	24_Corio.O_BA	24_Corio.O_BA_pos	Diet:BA | colonization:Corio_OligoMM	RAW_FILE_NAME=24_Corio.O_BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	25_Corio.O_HFD	25_Corio.O_HFD_pos	Diet:HFD | colonization:Corio_OligoMM	RAW_FILE_NAME=25_Corio.O_HFD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	27_Corio.O_HFD	27_Corio.O_HFD_pos	Diet:HFD | colonization:Corio_OligoMM	RAW_FILE_NAME=27_Corio.O_HFD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	28_Corio.O_HFD	28_Corio.O_HFD_pos	Diet:HFD | colonization:Corio_OligoMM	RAW_FILE_NAME=28_Corio.O_HFD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	29_Corio.O_HFD.BA	29_Corio.O_HFD.BA_pos	Diet:HFD.BA | colonization:Corio_OligoMM	RAW_FILE_NAME=29_Corio.O_HFD.BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	30_Corio.O_HFD.BA	30_Corio.O_HFD.BA_pos	Diet:HFD.BA | colonization:Corio_OligoMM	RAW_FILE_NAME=30_Corio.O_HFD.BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	31_Corio.O_HFD.BA	31_Corio.O_HFD.BA_pos	Diet:HFD.BA | colonization:Corio_OligoMM	RAW_FILE_NAME=31_Corio.O_HFD.BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	32_Corio.O_HFD.BA	32_Corio.O_HFD.BA_pos	Diet:HFD.BA | colonization:Corio_OligoMM	RAW_FILE_NAME=32_Corio.O_HFD.BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	33_Corio.O_CD	33_Corio.O_CD_pos	Diet:CD | colonization:Corio_OligoMM	RAW_FILE_NAME=33_Corio.O_CD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	34_Corio.O_CD	34_Corio.O_CD_pos	Diet:CD | colonization:Corio_OligoMM	RAW_FILE_NAME=34_Corio.O_CD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	35_Corio.O_CD	35_Corio.O_CD_pos	Diet:CD | colonization:Corio_OligoMM	RAW_FILE_NAME=35_Corio.O_CD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	36_O_CD	36_O_CD_pos	Diet:CD | colonization:OligoMM	RAW_FILE_NAME=36_O_CD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	37_O_CD	37_O_CD_pos	Diet:CD | colonization:OligoMM	RAW_FILE_NAME=37_O_CD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	38_O_CD	38_O_CD_pos	Diet:CD | colonization:OligoMM	RAW_FILE_NAME=38_O_CD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	39_O_HFD	39_O_HFD_pos	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=39_O_HFD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	40_O_HFD	40_O_HFD_pos	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=40_O_HFD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	41_O_HFD	41_O_HFD_pos	Diet:HFD | colonization:OligoMM	RAW_FILE_NAME=41_O_HFD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	42_O_BA	42_O_BA_pos	Diet:BA | colonization:OligoMM	RAW_FILE_NAME=42_O_BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	43_Corio.O_BA	43_Corio.O_BA_pos	Diet:BA | colonization:Corio_OligoMM	RAW_FILE_NAME=43_Corio.O_BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	44_Corio.O_BA	44_Corio.O_BA_pos	Diet:BA | colonization:Corio_OligoMM	RAW_FILE_NAME=44_Corio.O_BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	45_Corio.O_BA	45_Corio.O_BA_pos	Diet:BA | colonization:Corio_OligoMM	RAW_FILE_NAME=45_Corio.O_BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	46_O_CD	46_O_CD_pos	Diet:CD | colonization:OligoMM	RAW_FILE_NAME=46_O_CD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	47_Corio.O_HFD.BA	47_Corio.O_HFD.BA_pos	Diet:HFD.BA | colonization:Corio_OligoMM	RAW_FILE_NAME=47_Corio.O_HFD.BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	48_Corio.O_HFD.BA	48_Corio.O_HFD.BA_pos	Diet:HFD.BA | colonization:Corio_OligoMM	RAW_FILE_NAME=48_Corio.O_HFD.BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	49_Corio.O_HFD.BA	49_Corio.O_HFD.BA_pos	Diet:HFD.BA | colonization:Corio_OligoMM	RAW_FILE_NAME=49_Corio.O_HFD.BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	50_Corio.O_HFD	50_Corio.O_HFD_pos	Diet:HFD | colonization:Corio_OligoMM	RAW_FILE_NAME=50_Corio.O_HFD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	51_Corio.O_HFD	51_Corio.O_HFD_pos	Diet:HFD | colonization:Corio_OligoMM	RAW_FILE_NAME=51_Corio.O_HFD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	52_Corio.O_HFD	52_Corio.O_HFD_pos	Diet:HFD | colonization:Corio_OligoMM	RAW_FILE_NAME=52_Corio.O_HFD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	53_Corio.O_HFD	53_Corio.O_HFD_pos	Diet:HFD | colonization:Corio_OligoMM	RAW_FILE_NAME=53_Corio.O_HFD_pos.mzML
SUBJECT_SAMPLE_FACTORS           	54_O_BA	54_O_BA_pos	Diet:BA | colonization:OligoMM	RAW_FILE_NAME=54_O_BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	55_O_BA	55_O_BA_pos	Diet:BA | colonization:OligoMM	RAW_FILE_NAME=55_O_BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	56_O_BA	56_O_BA_pos	Diet:BA | colonization:OligoMM	RAW_FILE_NAME=56_O_BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	57_O_BA	57_O_BA_pos	Diet:BA | colonization:OligoMM	RAW_FILE_NAME=57_O_BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	58_O_HFD.BA	58_O_HFD.BA_pos	Diet:HFD.BA | colonization:OligoMM	RAW_FILE_NAME=58_O_HFD.BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	59_O_HFD.BA	59_O_HFD.BA_pos	Diet:HFD.BA | colonization:OligoMM	RAW_FILE_NAME=59_O_HFD.BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	60_O_HFD.BA	60_O_HFD.BA_pos	Diet:HFD.BA | colonization:OligoMM	RAW_FILE_NAME=60_O_HFD.BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	61_O_HFD.BA	61_O_HFD.BA_pos	Diet:HFD.BA | colonization:OligoMM	RAW_FILE_NAME=61_O_HFD.BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	422_Corio.O_BA	422_Corio.O_BA_pos	Diet:BA | colonization:Corio_OligoMM	RAW_FILE_NAME=422_Corio.O_BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	462_Corio.O_HFD.BA	462_Corio.O_HFD.BA_pos	Diet:HFD.BA | colonization:Corio_OligoMM	RAW_FILE_NAME=462_Corio.O_HFD.BA_pos.mzML
SUBJECT_SAMPLE_FACTORS           	11_6_O_BA	11_6_O_BA_pos	Diet:BA | colonization:OligoMM	RAW_FILE_NAME=11+6_O_BA_pos.mzML
#COLLECTION
CO:COLLECTION_SUMMARY            	Mice were euthanized with CO2 and colon was extracted.
CO:SAMPLE_TYPE                   	Colon
#TREATMENT
TR:TREATMENT_SUMMARY             	Germfree male C57BL/6N mice were associated with 0.2 ml of the freshly thawed
TR:TREATMENT_SUMMARY             	microbial mixtures via oral gavage three times within one week at the age of
TR:TREATMENT_SUMMARY             	five weeks. From week 8 onwards, diet was changed from autoclaved standard chow
TR:TREATMENT_SUMMARY             	to synthetic control diet (CD) for two weeks. Thereafter, the mice were randomly
TR:TREATMENT_SUMMARY             	assigned to four different groups fed the following diets for eight weeks (the
TR:TREATMENT_SUMMARY             	references and main differences in composition between the diets are provided in
TR:TREATMENT_SUMMARY             	Figure S1B): CD, control diet; CD-BA, control diet supplemented with the primary
TR:TREATMENT_SUMMARY             	bile acids cholic acid (CA) and chenodeoxycholic acid (CDCA) (each 0.1%); HFD,
TR:TREATMENT_SUMMARY             	lard-based high-fat diet (48% energy from fat); HFD-BA, HFD supplemented with CA
TR:TREATMENT_SUMMARY             	and CDCA. These diets were used due to the link between Coriobacteriia/E. lenta
TR:TREATMENT_SUMMARY             	and bile acid metabolism as well as host metabolic health.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Colon content was mixed with 5x ACN:H2O solvent and 4 steel beads for subsequent
SP:SAMPLEPREP_SUMMARY            	extraction in the bead mill. After centrifugation, 100 µL of the supernatant
SP:SAMPLEPREP_SUMMARY            	were mixed with 500 µL methanol:acetonitrile (ACN):water (2:3:1) followed by
SP:SAMPLEPREP_SUMMARY            	vortex and sonication. After centrifugation, 100 µL of the supernatant were
SP:SAMPLEPREP_SUMMARY            	dried in a SpeedVac™ vacuum concentrator (Eppendorf, Hamburg, Germany).
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity II
CH:COLUMN_NAME                   	Agilent ZORBAX Eclipse Plus C18 (100 x 2.1mm,1.8um)
CH:SOLVENT_A                     	Water + 0.1% FA
CH:SOLVENT_B                     	ACN + 0.1% FA
CH:FLOW_GRADIENT                 	0-5.5 min: 1% B; 5-20 min: 1-100% B; 20-22 min: 100% B; 22-22.5: 100-1% B;
CH:FLOW_GRADIENT                 	22.5-25 min: 1% B
CH:FLOW_RATE                     	0.3 ml/min
CH:COLUMN_TEMPERATURE            	45
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent 6546 QTOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	All samples were acquired in positive and negative ionization mode. The QTOF was
MS:MS_COMMENTS                   	set up in centroid mode with a scan range of 50 – 1000 m/z. After each full
MS:MS_COMMENTS                   	scan, the 2 most intense ions were subjected to fragmentation. The obtained raw
MS:MS_COMMENTS                   	data (.d-files) were imported into the Progenesis QI® software (version2.4,
MS:MS_COMMENTS                   	Waters Corporation) for further analysis.
MS:MS_RESULTS_FILE               	ST003011_AN004942_Results.txt	UNITS:Peak AUC	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END