#METABOLOMICS WORKBENCH syfoods_20240217_165608 DATATRACK_ID:4652 STUDY_ID:ST003094 ANALYSIS_ID:AN005064 PROJECT_ID:PR001923
VERSION             	1
CREATED_ON             	February 17, 2024, 8:52 pm
#PROJECT
PR:PROJECT_TITLE                 	Probiotic intervention of FMT mice
PR:PROJECT_SUMMARY               	L. plantarum intervention of gestational fecal microbiome transplant germ-free
PR:PROJECT_SUMMARY               	mice
PR:INSTITUTE                     	Beijing Sanyuan Foods Co. Ltd
PR:LAST_NAME                     	Bin
PR:FIRST_NAME                    	Liu
PR:ADDRESS                       	8th yingchang street, yinghai distric
PR:EMAIL                         	liubin@sanyuan.com.cn
PR:PHONE                         	01056306594
#STUDY
ST:STUDY_TITLE                   	probiotic on gestational health of FMT GF mice
ST:STUDY_SUMMARY                 	probiotic L.plantarum intervention gestational on FMT GF mice
ST:INSTITUTE                     	Beijing Sanyuan Foods Co. Ltd
ST:LAST_NAME                     	Liu
ST:FIRST_NAME                    	Bin
ST:ADDRESS                       	8th yingchang street, yinghai distric
ST:EMAIL                         	liubin@sanyuan.com.cn
ST:PHONE                         	01056306594
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENDER                        	Female
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	81	Sample source:serum | Factor:control	RAW_FILE_NAME=81.raw
SUBJECT_SAMPLE_FACTORS           	-	107	Sample source:serum | Factor:control	RAW_FILE_NAME=107.raw
SUBJECT_SAMPLE_FACTORS           	-	120	Sample source:serum | Factor:control	RAW_FILE_NAME=120.raw
SUBJECT_SAMPLE_FACTORS           	-	122	Sample source:serum | Factor:control	RAW_FILE_NAME=122.raw
SUBJECT_SAMPLE_FACTORS           	-	180	Sample source:serum | Factor:intervention	RAW_FILE_NAME=180.raw
SUBJECT_SAMPLE_FACTORS           	-	185	Sample source:serum | Factor:intervention	RAW_FILE_NAME=185.raw
SUBJECT_SAMPLE_FACTORS           	-	188	Sample source:serum | Factor:intervention	RAW_FILE_NAME=188.raw
SUBJECT_SAMPLE_FACTORS           	-	907	Sample source:serum | Factor:intervention	RAW_FILE_NAME=907.raw
SUBJECT_SAMPLE_FACTORS           	-	908	Sample source:serum | Factor:intervention	RAW_FILE_NAME=908.raw
SUBJECT_SAMPLE_FACTORS           	-	909	Sample source:serum | Factor:intervention	RAW_FILE_NAME=909.raw
#COLLECTION
CO:COLLECTION_SUMMARY            	Blood was collected and centrifuged at 3,000 rpm for 10 min and stored at -80°C
CO:COLLECTION_SUMMARY            	environment
CO:SAMPLE_TYPE                   	Blood (serum)
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	mice were randomly assigned into two experimental groups, named control and
TR:TREATMENT_SUMMARY             	intervention groups, receiving standard chow feeding, and standard chow + L.
TR:TREATMENT_SUMMARY             	plantarum
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Samples were vortex for 1 min after adding 0.4 mL methanol; then they were
SP:SAMPLEPREP_SUMMARY            	centrifuged for 10 min at 12,000 rpm at 4^oC, and the supernatant was dried and
SP:SAMPLEPREP_SUMMARY            	resolved using 0.15 mL 2-chloro-l-phenylalanine (4 ppm) solution, and then
SP:SAMPLEPREP_SUMMARY            	filtered by 0.22 μm membrane
SP:PROCESSING_STORAGE_CONDITIONS 	4℃
SP:EXTRACT_STORAGE               	-80℃
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	LC
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)
CH:SOLVENT_A                     	formic acid in water
CH:SOLVENT_B                     	formic acid in acetonitrile
CH:FLOW_GRADIENT                 	0~1 min, 2% B2; 1~9 min, 2%~50% B2; 9~12 min, 50%~98% B2; 12~13.5 min, 98% B2;
CH:FLOW_GRADIENT                 	13.5~14 min, 98%~2% B2; 14~20 min, 2% B2
CH:FLOW_RATE                     	0.25 mL/min
CH:COLUMN_TEMPERATURE            	40
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Orbitrap Exploris 480
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	MS1 range was 100-1000 m/z, and the number of data-dependent scans per cycle was
MS:MS_COMMENTS                   	4; MS/MS resolving power was 15000 FWHM, normalized collision energy was 30%,
MS:MS_COMMENTS                   	and dynamic exclusion time was automatic. The raw data were firstly converted to
MS:MS_COMMENTS                   	mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789)
MS:MS_COMMENTS                   	(Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al.,
MS:MS_COMMENTS                   	2020) for feature detection, retention time correction and alignment.
MS:MS_RESULTS_FILE               	ST003094_AN005064_Results.txt	UNITS:m/z values	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END