#METABOLOMICS WORKBENCH syfoods_20240217_215829 DATATRACK_ID:4653 STUDY_ID:ST003095 ANALYSIS_ID:AN005066 PROJECT_ID:PR001923 VERSION 1 CREATED_ON February 17, 2024, 11:17 pm #PROJECT PR:PROJECT_TITLE Probiotic intervention of FMT mice PR:PROJECT_SUMMARY L. plantarum intervention of gestational fecal microbiome transplant germ-free PR:PROJECT_SUMMARY mice PR:INSTITUTE Beijing Sanyuan Foods Co. Ltd PR:LAST_NAME Bin PR:FIRST_NAME Liu PR:ADDRESS 8th yingchang street, yinghai distric PR:EMAIL liubin@sanyuan.com.cn PR:PHONE 01056306594 #STUDY ST:STUDY_TITLE probiotic on gestational health of FMT GF mice ST:STUDY_SUMMARY probiotic L.plantarum intervention gestational on FMT GF mice ST:INSTITUTE Beijing Sanyuan Foods Co. Ltd ST:LAST_NAME Liu ST:FIRST_NAME Bin ST:ADDRESS 8th yingchang street, yinghai distric ST:EMAIL liubin@sanyuan.com.cn ST:PHONE 01056306594 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENDER Female #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - P220801156 Sample source:serum | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801156.mzML SUBJECT_SAMPLE_FACTORS - P220801157 Sample source:serum | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801157.mzML SUBJECT_SAMPLE_FACTORS - P220801158 Sample source:serum | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801158.mzML SUBJECT_SAMPLE_FACTORS - P220801159 Sample source:serum | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801159.mzML SUBJECT_SAMPLE_FACTORS - P220801160 Sample source:serum | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801160.mzML SUBJECT_SAMPLE_FACTORS - P220801161 Sample source:serum | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801161.mzML SUBJECT_SAMPLE_FACTORS - P220801162 Sample source:serum | Factor:control RAW_FILE_NAME=Wangyaru-P220801162.mzML SUBJECT_SAMPLE_FACTORS - P220801163 Sample source:serum | Factor:control RAW_FILE_NAME=Wangyaru-P220801163.mzML SUBJECT_SAMPLE_FACTORS - P220801164 Sample source:serum | Factor:control RAW_FILE_NAME=Wangyaru-P220801164.mzML SUBJECT_SAMPLE_FACTORS - P220801165 Sample source:serum | Factor:control RAW_FILE_NAME=Wangyaru-P220801165.mzML #COLLECTION CO:COLLECTION_SUMMARY Blood was collected and centrifuged at 3,000 rpm for 10 min and stored at -80°C CO:COLLECTION_SUMMARY environment CO:SAMPLE_TYPE Blood (serum) CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY mice were randomly assigned into two experimental groups, named control and TR:TREATMENT_SUMMARY intervention groups, receiving standard chow feeding, and standard chow + L. TR:TREATMENT_SUMMARY plantarum #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Samples were vortex for 1 min after adding 0.4 mL methanol; then they were SP:SAMPLEPREP_SUMMARY centrifuged for 10 min at 12,000 rpm at 4^oC, and the supernatant was dried and SP:SAMPLEPREP_SUMMARY resolved using 0.15 mL 2-chloro-l-phenylalanine (4 ppm) solution, and then SP:SAMPLEPREP_SUMMARY filtered by 0.22 μm membrane SP:PROCESSING_STORAGE_CONDITIONS 4℃ SP:EXTRACT_STORAGE -80℃ #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE LC CH:INSTRUMENT_NAME Thermo Dionex Ultimate 3000 CH:COLUMN_NAME Waters ACQUITY UPLC BEH HILIC (100 x 2.1mm,1.7um) CH:SOLVENT_A formic acid in water CH:SOLVENT_B formic acid in acetonitrile CH:FLOW_GRADIENT 0~1 min, 80% B; 1~2 min, 80~70% B; 2~2.5 min, 70% B; 2.5~3 min, 70~50% B; 3~3.5 CH:FLOW_GRADIENT min, 50% B; 3.5~4 min, 50~80% B; 4~6 min, 80% B CH:FLOW_RATE 0.4 mL/min CH:COLUMN_TEMPERATURE 40 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Quantiva QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS Multiple reaction monitoring (MRM) was employed. The raw data were firstly MS:MS_COMMENTS converted to mzXML format by MSConvert in the ProteoWizard software package MS:MS_COMMENTS (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara MS:MS_COMMENTS et al., 2020) for feature detection, retention time correction and alignment. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS μmol/L MS_METABOLITE_DATA_START Samples P220801162 P220801163 P220801164 P220801165 P220801160 P220801159 P220801157 P220801161 P220801156 P220801158 Factors Sample source:serum | Factor:control Sample source:serum | Factor:control Sample source:serum | Factor:control Sample source:serum | Factor:control Sample source:serum | Factor:intervention Sample source:serum | Factor:intervention Sample source:serum | Factor:intervention Sample source:serum | Factor:intervention Sample source:serum | Factor:intervention Sample source:serum | Factor:intervention choline 32.459841 28.537908 32.879674 28.777118 27.971415 31.257804 30.744923 36.689412 30.410424 28.887449 betaine 12.505261 14.050182 20.875248 14.600753 14.204114 18.598236 14.654962 24.629949 10.71293 8.166123 TMAO 0.481097 0.473859 0.977366 0.573132 0.39303 0.705887 0.394617 0.843455 0.707087 0.617149 creatinine 9.540114 8.929402 9.287823 7.752601 10.213967 8.506422 7.354962 18.395334 7.909091 8.218319 carnitine 12.989527 21.4473 26.015385 14.127252 13.243946 19.635126 18.847274 31.612201 13.765528 8.090083 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name retention index quantified m/z PubChem ID KEGG ID choline 0 104.2 305 C00114 betaine 0 118.1 247 C00719 TMAO 0 75.9 1145 C01104 creatinine 0 114 588 C00791 carnitine 0 162 288 C00487 METABOLITES_END #END