#METABOLOMICS WORKBENCH syfoods_20240217_232236 DATATRACK_ID:4654 STUDY_ID:ST003096 ANALYSIS_ID:AN005067 PROJECT_ID:PR001923 VERSION 1 CREATED_ON February 18, 2024, 12:05 am #PROJECT PR:PROJECT_TITLE Probiotic intervention of FMT mice PR:PROJECT_SUMMARY L. plantarum intervention of gestational fecal microbiome transplant germ-free PR:PROJECT_SUMMARY mice PR:INSTITUTE Beijing Sanyuan Foods Co. Ltd PR:LAST_NAME Bin PR:FIRST_NAME Liu PR:ADDRESS 8th yingchang street, yinghai distric PR:EMAIL liubin@sanyuan.com.cn PR:PHONE 01056306594 #STUDY ST:STUDY_TITLE probiotic on gestational health of FMT GF mice ST:STUDY_SUMMARY probiotic L.plantarum intervention gestational on FMT GF mice ST:INSTITUTE Beijing Sanyuan Foods Co. Ltd ST:LAST_NAME Liu ST:FIRST_NAME Bin ST:ADDRESS 8th yingchang street, yinghai distric ST:EMAIL liubin@sanyuan.com.cn ST:PHONE 01056306594 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENDER Female #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - 81 Sample source:cecum content | Factor:control RAW_FILE_NAME=81.mzML SUBJECT_SAMPLE_FACTORS - 107 Sample source:cecum content | Factor:control RAW_FILE_NAME=107.mzML SUBJECT_SAMPLE_FACTORS - 120 Sample source:cecum content | Factor:control RAW_FILE_NAME=120.mzML SUBJECT_SAMPLE_FACTORS - 122 Sample source:cecum content | Factor:control RAW_FILE_NAME=122.mzML SUBJECT_SAMPLE_FACTORS - 180 Sample source:cecum content | Factor:intervention RAW_FILE_NAME=180.mzML SUBJECT_SAMPLE_FACTORS - 185 Sample source:cecum content | Factor:intervention RAW_FILE_NAME=185.mzML SUBJECT_SAMPLE_FACTORS - 188 Sample source:cecum content | Factor:intervention RAW_FILE_NAME=188.mzML SUBJECT_SAMPLE_FACTORS - 907 Sample source:cecum content | Factor:intervention RAW_FILE_NAME=907.mzML SUBJECT_SAMPLE_FACTORS - 908 Sample source:cecum content | Factor:intervention RAW_FILE_NAME=908.mzML SUBJECT_SAMPLE_FACTORS - 909 Sample source:cecum content | Factor:intervention RAW_FILE_NAME=909.mzML #COLLECTION CO:COLLECTION_SUMMARY Blood was collected and centrifuged at 3,000 rpm for 10 min and stored at -80°C CO:COLLECTION_SUMMARY environment CO:SAMPLE_TYPE Cecum CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY mice were randomly assigned into two experimental groups, named control and TR:TREATMENT_SUMMARY intervention groups, receiving standard chow feeding, and standard chow + L. TR:TREATMENT_SUMMARY plantarum #SAMPLEPREP SP:SAMPLEPREP_SUMMARY 100 mg of sample was extracted in 50 μL of 15% phosphoric acid with 100 μL of SP:SAMPLEPREP_SUMMARY 125 μg/mL 4-methyl valeric acid solution as internal standard and 400 μL SP:SAMPLEPREP_SUMMARY ether, and were centrifuged at 4 °C for 10 min at 12000 rpm after vortexing for SP:SAMPLEPREP_SUMMARY 1 min and the supernatant was transferred into the vial SP:PROCESSING_STORAGE_CONDITIONS 4℃ SP:EXTRACT_STORAGE -80℃ #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Thermo Trace 1310 CH:COLUMN_NAME Agilent HP-INNOWAX (30 m × 0.25 mm ID × 0.25 μm) CH:SOLVENT_A none CH:SOLVENT_B none CH:FLOW_GRADIENT none CH:FLOW_RATE 1 mL/min CH:COLUMN_TEMPERATURE 90 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo ISQ LT MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE UNSPECIFIED MS:MS_COMMENTS Single ion monitoring (SIM) mode was used with the electron energy of 70 eV.MS MS:MS_COMMENTS Convert software (Proteowizard, v3.0.8789) was applied to convert “.raw” MS:MS_COMMENTS format raw data to “.mzML” format, which would be used for downstream MS:MS_COMMENTS analysis. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS μg/g MS_METABOLITE_DATA_START Samples 908 188 909 185 180 907 81 107 120 122 Factors Sample source:cecum content | Factor:intervention Sample source:cecum content | Factor:intervention Sample source:cecum content | Factor:intervention Sample source:cecum content | Factor:intervention Sample source:cecum content | Factor:intervention Sample source:cecum content | Factor:intervention Sample source:cecum content | Factor:control Sample source:cecum content | Factor:control Sample source:cecum content | Factor:control Sample source:cecum content | Factor:control Isobutyric acid 21.1868 25.8283 21.0328 22.4477 20.1322 26.0719 20.7403 15.6469 15.3941 31.5321 Acetic acid 908.0426 765.4406 776.1381 985.9095 860.3189 927.8182 824.4127 814.7261 1359.5274 1072.7055 Butyric acid 409.4917 157.2953 166.0075 572.9804 240.3079 223.751 136.8884 140.7733 858.9547 516.5629 Isovaleric acid 13.9153 18.6662 18.6383 16.795 17.2469 21.9251 16.377 10.2744 9.4717 22.7542 Valeric acid 58.6347 33.7477 33.4303 62.5157 35.8674 36.7059 25.7836 22.4222 54.6714 65.3222 Caproic_acid 1.4126 1.3961 1.4772 1.5641 1.7061 1.7051 1.8658 1.7499 Propionic acid 385.3268 236.7324 315.5355 458.8674 271.14 335.4233 241.0505 182.3167 447.7459 420.8304 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name retention index quantified m/z PubChem ID KEGG ID Isobutyric acid 6.1 0 6590 C02632 Acetic acid 4.64 0 176 C00033 Butyric acid 6.98 0 264 C00246 Isovaleric acid 7.64 0 10430 C08262 Valeric acid 8.77 0 7991 C00803 Caproic_acid 10.23 0 8892 C01585 Propionic acid 5.7 0 1032 D02310 METABOLITES_END #END