#METABOLOMICS WORKBENCH johru_20240219_051021 DATATRACK_ID:4663 STUDY_ID:ST003105 ANALYSIS_ID:AN005084 PROJECT_ID:PR001928
VERSION             	1
CREATED_ON             	February 19, 2024, 5:35 am
#PROJECT
PR:PROJECT_TITLE                 	Activation of GFRAL+ Neurons Induces Hypothermia and Glucoregulatory Responses
PR:PROJECT_TITLE                 	Associated with Nausea and Torpor
PR:PROJECT_SUMMARY               	GFRAL-expressing neurons actuate aversion and nausea, are targets for obesity
PR:PROJECT_SUMMARY               	treatment and may mediate metformin effects by long-term GDF15-GFRAL agonism. If
PR:PROJECT_SUMMARY               	GFRAL+ neurons acutely regulate glucose and energy homeostasis is however
PR:PROJECT_SUMMARY               	underexplored. Here, we report that cell-specific activation of GFRAL+ neurons
PR:PROJECT_SUMMARY               	using a variety of techniques causes a torpor-like state, including hypothermia,
PR:PROJECT_SUMMARY               	the release of stress hormones, a shift from glucose to lipid oxidation, and
PR:PROJECT_SUMMARY               	impaired insulin sensitivity, glucose tolerance and skeletal muscle glucose
PR:PROJECT_SUMMARY               	uptake but augmented glucose uptake in visceral fat. Metabolomic analysis of
PR:PROJECT_SUMMARY               	blood and transcriptomics of muscle and fat indicate alterations in ketogenesis,
PR:PROJECT_SUMMARY               	insulin signaling, adipose tissue differentiation and mitogenesis, and energy
PR:PROJECT_SUMMARY               	fluxes. Our findings reveal that acute GFRAL+ neuron activation induces
PR:PROJECT_SUMMARY               	endocrine and gluco- and thermoregulatory responses associated with nausea and
PR:PROJECT_SUMMARY               	torpor. While chronic activation of GFRAL signaling promotes weight loss in
PR:PROJECT_SUMMARY               	obesity, these results show that acute activation of GFRAL+ neurons causes
PR:PROJECT_SUMMARY               	hypothermia and hyperglycemia.
PR:INSTITUTE                     	University of Gothenburg
PR:LAST_NAME                     	Ruud
PR:FIRST_NAME                    	Johan
PR:ADDRESS                       	Medicinaregatan 11
PR:EMAIL                         	johan.ruud@gu.se
PR:PHONE                         	+46766186879
#STUDY
ST:STUDY_TITLE                   	Activation of GFRAL+ Neurons Induces Hypothermia and Glucoregulatory Responses
ST:STUDY_TITLE                   	Associated with Nausea and Torpor
ST:STUDY_SUMMARY                 	GFRAL-expressing neurons actuate aversion and nausea, are targets for obesity
ST:STUDY_SUMMARY                 	treatment and may mediate metformin effects by long-term GDF15-GFRAL agonism. If
ST:STUDY_SUMMARY                 	GFRAL+ neurons acutely regulate glucose and energy homeostasis is however
ST:STUDY_SUMMARY                 	underexplored. Here, we report that cell-specific activation of GFRAL+ neurons
ST:STUDY_SUMMARY                 	using a variety of techniques causes a torpor-like state, including hypothermia,
ST:STUDY_SUMMARY                 	the release of stress hormones, a shift from glucose to lipid oxidation, and
ST:STUDY_SUMMARY                 	impaired insulin sensitivity, glucose tolerance and skeletal muscle glucose
ST:STUDY_SUMMARY                 	uptake but augmented glucose uptake in visceral fat. Metabolomic analysis of
ST:STUDY_SUMMARY                 	blood and transcriptomics of muscle and fat indicate alterations in ketogenesis,
ST:STUDY_SUMMARY                 	insulin signaling, adipose tissue differentiation and mitogenesis, and energy
ST:STUDY_SUMMARY                 	fluxes. Our findings reveal that acute GFRAL+ neuron activation induces
ST:STUDY_SUMMARY                 	endocrine and gluco- and thermoregulatory responses associated with nausea and
ST:STUDY_SUMMARY                 	torpor. While chronic activation of GFRAL signaling promotes weight loss in
ST:STUDY_SUMMARY                 	obesity, these results show that acute activation of GFRAL+ neurons causes
ST:STUDY_SUMMARY                 	hypothermia and hyperglycemia.
ST:INSTITUTE                     	University of Gothenburg
ST:LAST_NAME                     	Ruud
ST:FIRST_NAME                    	Johan
ST:ADDRESS                       	Medicinaregatan 11
ST:EMAIL                         	johan.ruud@gu.se
ST:PHONE                         	+46766186879
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	blood1_cage1	2	Sample source:Plasma | Genotype:Wild-type	Treatment=CNO_6h; RAW_FILE_NAME=2
SUBJECT_SAMPLE_FACTORS           	blood1_cage3	4	Sample source:Plasma | Genotype:Transgenic	Treatment=CNO_6h; RAW_FILE_NAME=4
SUBJECT_SAMPLE_FACTORS           	blood1_cage4	6	Sample source:Plasma | Genotype:Wild-type	Treatment=CNO_6h; RAW_FILE_NAME=6
SUBJECT_SAMPLE_FACTORS           	blood1_cage5	8	Sample source:Plasma | Genotype:Wild-type	Treatment=CNO_6h; RAW_FILE_NAME=8
SUBJECT_SAMPLE_FACTORS           	blood1_cage6	10	Sample source:Plasma | Genotype:Wild-type	Treatment=CNO_6h; RAW_FILE_NAME=10
SUBJECT_SAMPLE_FACTORS           	blood1_cage8	12	Sample source:Plasma | Genotype:Transgenic	Treatment=CNO_6h; RAW_FILE_NAME=12
SUBJECT_SAMPLE_FACTORS           	blood1_cage9	14	Sample source:Plasma | Genotype:Wild-type	Treatment=CNO_6h; RAW_FILE_NAME=14
SUBJECT_SAMPLE_FACTORS           	blood1_cage10	16	Sample source:Plasma | Genotype:Transgenic	Treatment=CNO_6h; RAW_FILE_NAME=16
SUBJECT_SAMPLE_FACTORS           	blood1_cage13	18	Sample source:Plasma | Genotype:Transgenic	Treatment=CNO_6h; RAW_FILE_NAME=18
SUBJECT_SAMPLE_FACTORS           	blood1_cage14	20	Sample source:Plasma | Genotype:Transgenic	Treatment=CNO_6h; RAW_FILE_NAME=20
SUBJECT_SAMPLE_FACTORS           	blood1_cage16	22	Sample source:Plasma | Genotype:Wild-type	Treatment=CNO_6h; RAW_FILE_NAME=22
SUBJECT_SAMPLE_FACTORS           	blood1_cage18	24	Sample source:Plasma | Genotype:Transgenic	Treatment=CNO_6h; RAW_FILE_NAME=24
SUBJECT_SAMPLE_FACTORS           	blood1_cage21	26	Sample source:Plasma | Genotype:Wild-type	Treatment=CNO_6h; RAW_FILE_NAME=26
SUBJECT_SAMPLE_FACTORS           	blood1_cage22	28	Sample source:Plasma | Genotype:Transgenic	Treatment=CNO_6h; RAW_FILE_NAME=28
SUBJECT_SAMPLE_FACTORS           	blood1_cage24	30	Sample source:Plasma | Genotype:Transgenic	Treatment=CNO_6h; RAW_FILE_NAME=30
SUBJECT_SAMPLE_FACTORS           	blood1_cage25	32	Sample source:Plasma | Genotype:Wild-type	Treatment=CNO_6h; RAW_FILE_NAME=32
SUBJECT_SAMPLE_FACTORS           	1.1inca	34	Sample source:Plasma | Genotype:Wild-type	Treatment=CNO_6h; RAW_FILE_NAME=34
SUBJECT_SAMPLE_FACTORS           	1.2inca	36	Sample source:Plasma | Genotype:Transgenic	Treatment=CNO_6h; RAW_FILE_NAME=36
SUBJECT_SAMPLE_FACTORS           	1.3inca	38	Sample source:Plasma | Genotype:Wild-type	Treatment=CNO_6h; RAW_FILE_NAME=38
SUBJECT_SAMPLE_FACTORS           	1.4inca	40	Sample source:Plasma | Genotype:Transgenic	Treatment=CNO_6h; RAW_FILE_NAME=40
SUBJECT_SAMPLE_FACTORS           	1.5inca	42	Sample source:Plasma | Genotype:Transgenic	Treatment=CNO_6h; RAW_FILE_NAME=42
SUBJECT_SAMPLE_FACTORS           	1.6inca	44	Sample source:Plasma | Genotype:Wild-type	Treatment=CNO_6h; RAW_FILE_NAME=44
SUBJECT_SAMPLE_FACTORS           	1.7inca	46	Sample source:Plasma | Genotype:Transgenic	Treatment=CNO_6h; RAW_FILE_NAME=46
SUBJECT_SAMPLE_FACTORS           	1.8inca	48	Sample source:Plasma | Genotype:Wild-type	Treatment=CNO_6h; RAW_FILE_NAME=48
SUBJECT_SAMPLE_FACTORS           	2.1inca	50	Sample source:Plasma | Genotype:Wild-type	Treatment=CNO_6h; RAW_FILE_NAME=50
SUBJECT_SAMPLE_FACTORS           	2.2inca	52	Sample source:Plasma | Genotype:Transgenic	Treatment=CNO_6h; RAW_FILE_NAME=52
SUBJECT_SAMPLE_FACTORS           	2.3inca	54	Sample source:Plasma | Genotype:Transgenic	Treatment=CNO_6h; RAW_FILE_NAME=54
SUBJECT_SAMPLE_FACTORS           	2.4inca	56	Sample source:Plasma | Genotype:Wild-type	Treatment=CNO_6h; RAW_FILE_NAME=56
SUBJECT_SAMPLE_FACTORS           	2.5inca	58	Sample source:Plasma | Genotype:Transgenic	Treatment=CNO_6h; RAW_FILE_NAME=58
SUBJECT_SAMPLE_FACTORS           	2.6inca	60	Sample source:Plasma | Genotype:Wild-type	Treatment=CNO_6h; RAW_FILE_NAME=60
SUBJECT_SAMPLE_FACTORS           	2.7inca	62	Sample source:Plasma | Genotype:Transgenic	Treatment=CNO_6h; RAW_FILE_NAME=62
SUBJECT_SAMPLE_FACTORS           	2.8inca	64	Sample source:Plasma | Genotype:Wild-type	Treatment=CNO_6h; RAW_FILE_NAME=64
#COLLECTION
CO:COLLECTION_SUMMARY            	Blood was collected in EDTA-coated tubes, and plasma was obtained through
CO:COLLECTION_SUMMARY            	centrifugation (7000 x g, 7 min, 4°C), snap frozen in liquid nitrogen and
CO:COLLECTION_SUMMARY            	stored at -80°C until further use.
CO:SAMPLE_TYPE                   	Blood (plasma)
#TREATMENT
TR:TREATMENT_SUMMARY             	Control mice (wild-type) and mice expressing hM3Dq in GFRAL neurons (transgenic)
TR:TREATMENT_SUMMARY             	were given CNO (1 mg/kg) i.p. and bled 6 hours later for NMR metabolomics.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Plasma was thawed at room temperature for 15 min before transferring 150 µl to
SP:SAMPLEPREP_SUMMARY            	pre-washed ultrafiltration tubes (Amicon Ultra 0.5 ml, 3k MWCO, Merck Millipore)
SP:SAMPLEPREP_SUMMARY            	which were spun at 14000 x g, at 4°C for 90 min. 90 µl of each filtrate was
SP:SAMPLEPREP_SUMMARY            	mixed with 90 µl buffer (75 mM sodium phosphate pH 7.4, 0.1% w/v sodium azide,
SP:SAMPLEPREP_SUMMARY            	3.66 mM 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid (TSPd4), in 20% v/v D2O) and
SP:SAMPLEPREP_SUMMARY            	transferred to 3 mm SampleJet NMR-tubes.
#ANALYSIS
AN:ANALYSIS_TYPE                 	NMR
#NMR
NM:INSTRUMENT_NAME               	Bruker Neo
NM:INSTRUMENT_TYPE               	FT-NMR
NM:NMR_EXPERIMENT_TYPE           	1D-1H
NM:SPECTROMETER_FREQUENCY        	599.75 MHz
#NMR_METABOLITE_DATA
NMR_METABOLITE_DATA:UNITS	mM
NMR_METABOLITE_DATA_START
Samples	2	4	6	8	10	12	14	16	18	20	22	24	26	28	30	32	34	36	38	40	42	44	46	48	50	52	54	56	58	60	62	64
Factors	Sample source:Plasma | Genotype:Wild-type	Sample source:Plasma | Genotype:Transgenic	Sample source:Plasma | Genotype:Wild-type	Sample source:Plasma | Genotype:Wild-type	Sample source:Plasma | Genotype:Wild-type	Sample source:Plasma | Genotype:Transgenic	Sample source:Plasma | Genotype:Wild-type	Sample source:Plasma | Genotype:Transgenic	Sample source:Plasma | Genotype:Transgenic	Sample source:Plasma | Genotype:Transgenic	Sample source:Plasma | Genotype:Wild-type	Sample source:Plasma | Genotype:Transgenic	Sample source:Plasma | Genotype:Wild-type	Sample source:Plasma | Genotype:Transgenic	Sample source:Plasma | Genotype:Transgenic	Sample source:Plasma | Genotype:Wild-type	Sample source:Plasma | Genotype:Wild-type	Sample source:Plasma | Genotype:Transgenic	Sample source:Plasma | Genotype:Wild-type	Sample source:Plasma | Genotype:Transgenic	Sample source:Plasma | Genotype:Transgenic	Sample source:Plasma | Genotype:Wild-type	Sample source:Plasma | Genotype:Transgenic	Sample source:Plasma | Genotype:Wild-type	Sample source:Plasma | Genotype:Wild-type	Sample source:Plasma | Genotype:Transgenic	Sample source:Plasma | Genotype:Transgenic	Sample source:Plasma | Genotype:Wild-type	Sample source:Plasma | Genotype:Transgenic	Sample source:Plasma | Genotype:Wild-type	Sample source:Plasma | Genotype:Transgenic	Sample source:Plasma | Genotype:Wild-type
2-hydroxybutyrate	00034	00144	0004	00034	00026	00112	00038	0017	00086	00136	00028	00096	00032	0016	00146	00034	00058	00282	00056	00266	005	00068	00274	0011	00058	0023	00192	00068	0012	00038	00244	00038
2-Hydroxyisobutyrate	0007	00106	00102	00074	00096	00076	00086	00086	00114	0011	00068	00078	00086	0012	00108	0008	00096	00146	00076	00176	00132	0008	00116	00086	00098	00104	00096	00082	00126	0009	00138	00076
2-Hydroxyisovalerate	00088	00096	001	00066	00066	00086	00088	00088	00086	001	00068	001	00086	00112	00104	0007	0005	00104	00074	00124	0011	00068	00124	00054	00072	001	0009	0007	00096	0006	00104	00056
2-Oxoisocaproate	00034	00048	00042	0004	00046	00042	0004	00054	00028	00034	00026	00036	0004	00038	00022	0004	00066	00062	00046	00056	00034	00042	00036	00048	00028	00036	0003	00034	0002	00026	0003	00032
3-Hydroxybutyrate	00586	02224	00842	00632	01114	01878	00692	02372	01346	04044	00474	02278	00622	01702	03046	01092	0115	01612	0082	02588	01056	00528	01336	00538	00434	01052	0083	0059	00764	00486	0082	00154
3-Hydroxyisobutyrate	002	00418	00238	00132	00256	00408	0018	0036	00332	00404	00126	00386	00164	0043	00404	00238	003	0053	0024	00636	00416	00252	00448	00236	00164	00456	0036	00224	00302	00148	00276	00162
3-Methyl-2-oxovalerate	00024	00038	00014	00024	0002	00022	00026	00026	00018	0002	00014	00028	00028	00026	00026	0002	00038	0005	00036	0004	00044	0002	00026	00022	00008	0002	00024	00016	00022	00014	00026	00016
Acetate	01818	02834	01662	01654	02116	03164	01602	01844	02412	0206	02072	02698	02124	02048	0157	01298	04282	02266	01838	02146	02378	01898	04542	01064	03656	01656	02526	02466	0341	02894	03372	04404
Acetoacetate	00064	00138	0008	0005	00106	00206	0003	00228	00096	00348	00046	00228	0003	00202	0021	00068	00112	00328	00058	00374	0024	00046	00206	00026	0002	0004	00032	00026	00106	0003	003	00002
Acetone	00056	00156	00054	00056	00048	001	00058	00084	00098	0013	00066	00114	0006	00092	00112	00054	00088	00194	00072	0023	00118	001	00162	00046	001	00078	00086	00068	00124	00074	00138	00306
Alanine	03826	03186	03546	03178	03388	02336	03682	0245	0257	02554	03026	02482	0245	02416	02342	03094	03478	0289	0465	02514	02802	03244	02406	05656	03458	0378	0354	04716	02258	03254	02414	03102
Betaine	0044	0042	00322	00366	00636	00438	00398	0044	00462	00446	00392	00602	0044	00388	00596	00604	00536	00628	00522	00684	00456	0044	00524	00534	0038	0103	00576	00352	0054	00398	00502	00428
Butyrate	00066	00046	00052	00044	0005	00058	00052	00066	00032	00046	00032	00042	00048	0005	00044	00038	0007	00064	00056	0007	00072	00046	00056	00034	00036	00052	0004	00036	0003	0002	00034	00024
Carnitine	0023	00174	00288	00238	00244	00162	00252	00252	00252	00184	00252	00178	00242	00166	00122	0029	00252	00156	00282	00178	00146	00252	00276	00386	0023	00154	00198	00252	00174	0019	00194	00214
Citrate	03308	02734	02966	02358	0251	02012	02778	02244	02218	0212	03052	01882	02554	02248	02216	02726	02674	01896	02906	02006	0247	02438	0155	03656	02598	01952	01826	02554	01788	01886	01432	02412
Creatine	01034	01174	00958	0107	02208	01164	01062	01054	00848	01292	01004	01062	00944	01004	00988	01148	01024	01094	00992	01058	01412	00964	01678	014	01008	01586	01306	01168	01148	00872	01536	01106
Creatinine	00104	00072	0006	00054	00038	00042	00092	00092	0007	0008	00064	00062	00058	00132	0008	00084	00128	00078	00136	00086	00138	00082	00126	001	00082	00094	0009	0012	00086	00076	00078	00116
Dimethylamine	0045	05754	0271	03424	00312	02452	0697	0045	03168	07068	08268	03098	0169	10446	04708	003	00062	03064	01662	03388	1154	02092	0797	00102	03722	07164	01796	0052	04978	02116	07938	0799
Dimethyl sulfone	13676	0749	17508	17782	13272	10914	23174	0512	06618	05956	10852	06928	12294	03136	05134	15792	16268	03702	1638	03974	02854	13706	04712	14978	15386	04068	06544	13302	0399	127	02818	12302
Ethanol	40412	185696	84988	77224	273216	179132	11192	130248	189362	10587	99732	122702	66864	12603	21929	161726	104052	155448	63714	190384	86302	10565	151164	38428	55246	3863	45144	35446	37376	52032	38258	16452
Formate	00924	01004	00942	00956	01466	01066	00924	00854	00972	01096	0093	01074	00922	01108	00952	01014	0177	02018	01408	01678	01634	01436	01738	0134	01664	01778	01772	01602	01518	01652	0202	02644
Fumarate	00072	0007	00074	00062	00076	00062	00092	00054	0006	00056	00066	00052	00082	00032	00054	00082	0004	00024	00064	00038	0003	00058	00024	00142	00046	00048	00036	00064	00028	0003	00014	00036
Glucose	121398	1339	120634	105154	105188	118576	125102	11704	102124	117274	97412	110458	112012	123902	104128	11388	141778	141654	14322	136776	124554	157656	130242	191574	175116	174204	16752	150828	169882	162382	146038	14141
Glutamine	0513	04318	04526	0426	03992	0325	0502	0323	03342	03316	03936	03676	04762	02994	03188	04608	04482	02694	05044	02932	0291	04244	02574	0522	04298	03348	03492	04204	02456	03986	02264	04438
Glycerol	05026	05628	04954	05904	0942	05312	0732	0521	05136	05044	05314	04902	05262	06784	06358	06248	08758	07028	12364	08588	05976	06806	09696	07304	09826	06198	02752	03396	02268	0205	02158	04218
Glycine	01796	01306	01898	01646	01572	00996	0161	01028	01094	01186	01368	01286	01666	01448	0129	01822	01962	0114	02272	01248	01224	014	01052	02444	01616	01456	01312	01964	0105	01688	01472	01704
Histidine	00528	0054	00494	00428	00298	00444	00518	00494	00414	00552	00392	00508	00492	00576	0045	0053	00692	00556	00696	0061	00678	00522	00546	00706	0057	00646	00538	00578	00462	005	0056	00574
Isoleucine	00664	01058	00642	00572	00516	00814	0066	01034	00858	01258	00532	0093	00676	0116	01114	00538	00794	01184	0078	01018	01248	00606	0093	00768	00582	01218	00976	0066	009	00566	01336	00474
Isopropanol	02488	1265	05	0449	17776	09596	06724	08	11918	06704	05716	07624	03822	07808	13852	0993	02162	02558	00972	04322	01566	01496	0289	0058	00778	00872	00732	00662	00796	00748	08576	05834
Lactate	5386	52926	37478	41018	58896	38516	56734	44024	40272	34474	49386	33342	52132	37824	37606	47996	4532	285	59508	28688	27558	51754	247	75194	29182	39956	33466	41276	1797	22824	1276	27094
Leucine	01116	01864	01094	00928	009	01476	01064	01736	01472	0227	0083	01634	01146	02076	01916	00906	0127	01974	01278	01862	02068	0098	01708	01172	00924	02084	01578	0125	01422	00942	02178	0081
Lysine	01728	01108	01498	011	01696	00952	015	01396	0121	01606	01166	0152	01406	01602	01322	01412	01896	01664	01686	01216	01416	01306	01138	01998	01362	01676	01442	01734	01222	01266	01544	01676
Malate	00674	00432	0053	00494	00548	00338	00614	00396	00284	00306	00348	00472	00486	00264	0037	00466	00696	00144	00614	00254	00146	00412	00392	01386	00228	00258	00222	00622	00184	00262	00148	00218
Methanol	0037	00332	00134	00238	00392	00298	00324	00404	0021	0039	00386	00486	00522	00448	00434	0036	00364	00364	0035	00322	00212	00284	00134	00358	00324	0028	00292	00354	00262	00266	00214	00208
Methionine	00408	00294	00402	0033	00384	00246	00368	00266	00238	00318	00304	00292	00364	00286	003	004	00406	00344	00362	0033	00382	00354	0035	00482	00334	00404	00342	00588	0025	0034	00322	00364
Phenylalanine	00546	00744	00466	00382	0044	00572	00542	0066	00614	0082	0037	00622	0044	00786	007	00534	00706	00812	00638	00932	00792	00558	0076	0066	00476	01012	00756	00614	00618	00496	00762	00482
Proline	00498	00242	0042	00374	00522	00256	0042	00392	00206	00374	00348	00392	00554	00456	00352	0053	00824	00424	0072	00344	0028	00496	00392	0075	00356	00502	0045	00846	00282	00312	0036	00398
Propionate	00036	00022	00046	00042	0006	00056	00026	00052	00044	00044	00022	00032	0004	0005	0004	00038	00044	00042	00038	00024	0003	00036	00052	00062	00038	00034	0003	0003	00038	0002	00034	0002
Pyruvate	0198	01564	018	0182	01988	01502	02252	01566	01392	01086	01654	01214	0182	01124	00934	01634	0145	01132	01708	0111	0097	01586	00726	0163	00952	01098	00826	01394	00564	0088	00466	01332
Sarcosine	0008	00058	00064	0006	00096	00134	00088	00078	00078	00068	00078	001	0007	00066	0006	0008	00126	0014	00128	00154	00088	00106	00102	00124	00128	00162	00144	00146	00114	0011	001	00168
Serine	0128	01092	01124	01216	0119	0117	0087	00648	01072	00952	00822	00496	00862	01072	00856	01422	01488	01052	01212	0163	01344	01134	02126	01326	01166	01814	01156	01586	01078	01042	01534	00984
Succinate	003	00494	00288	00264	00314	0021	00414	00226	00204	00338	00314	00352	00448	00248	00454	00558	00314	00136	00624	002	00134	00272	00104	01966	00216	00464	0024	00398	00098	00142	00058	00136
Taurine	06124	11668	03522	03952	06182	05834	03892	06124	04482	07166	03776	05398	03474	0792	06562	04422	06916	15278	06124	14078	07956	04906	08754	06124	05562	14232	12536	06124	0857	03704	11972	04716
Threonine	01016	00672	00878	00792	00538	00862	00476	0053	00614	00594	00626	00568	01002	01158	00726	00696	01296	00906	01414	0052	01026	00922	00398	01104	00932	00772	00706	009	00876	0072	0058	0098
Tyrosine	00654	0055	00704	00586	00618	00502	00772	00486	00476	00586	0049	0051	00594	00568	0054	00872	00764	0055	00808	00566	00568	007	00512	01046	00614	00804	00562	00988	0046	00722	00548	00762
Urea	28512	34904	27938	21198	33054	21878	2658	2279	28214	31298	28456	29516	22272	38348	28238	30638	3525	27782	28434	29388	31842	26146	28722	27788	28082	35388	25668	3422	3246	30704	29686	25148
Uridine	00072	00078	00038	00042	00066	00058	0005	00054	00046	00068	00048	00052	00052	00036	00062	0005	0005	00036	00092	0002	0002	00078	00048	00144	00086	00078	00076	00102	00028	00106	00028	00038
Valerate	0003	00012	00026	00014	00008	0002	00024	00016	00016	00024	00012	00024	00016	00036	00024	00026	00186	00242	00152	00266	0028	00218	00114	0009	00108	00076	00096	0006	00064	00096	00038	00022
Valine	01404	02358	01442	01272	01108	0165	01434	02446	0187	02636	0112	02164	01516	02628	0247	0127	01666	02538	01754	02298	02772	01366	0229	01682	01264	02618	02188	01562	02018	01198	02732	0119
NMR_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
2-hydroxybutyrate
2-Hydroxyisobutyrate
2-Hydroxyisovalerate
2-Oxoisocaproate
3-Hydroxybutyrate
3-Hydroxyisobutyrate
3-Methyl-2-oxovalerate
Acetate
Acetoacetate
Acetone
Alanine
Betaine
Butyrate
Carnitine
Citrate
Creatine
Creatinine
Dimethylamine
Dimethyl sulfone
Ethanol
Formate
Fumarate
Glucose
Glutamine
Glycerol
Glycine
Histidine
Isoleucine
Isopropanol
Lactate
Leucine
Lysine
Malate
Methanol
Methionine
Phenylalanine
Proline
Propionate
Pyruvate
Sarcosine
Serine
Succinate
Taurine
Threonine
Tyrosine
Urea
Uridine
Valerate
Valine
METABOLITES_END
#END